CN106982736A - A kind of breeding method of Tissue culture the seedling of grape - Google Patents
A kind of breeding method of Tissue culture the seedling of grape Download PDFInfo
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- CN106982736A CN106982736A CN201710297132.9A CN201710297132A CN106982736A CN 106982736 A CN106982736 A CN 106982736A CN 201710297132 A CN201710297132 A CN 201710297132A CN 106982736 A CN106982736 A CN 106982736A
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- seedling
- grape
- culture
- breeding method
- tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
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- Cell Biology (AREA)
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- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a kind of breeding method of Tissue culture the seedling of grape, the seed of grape is soaked in the liquor potassic permanganate that mass concentration is 1 3%, is rinsed afterwards with sterilized water, obtains aseptic seed;The aseptic seed is inoculated into Initial culture base, Initial culture is carried out, obtains aseptic seedling;Using the cotyledon of the aseptic seedling and hypocotyl as explant, and it is inoculated in subculture medium, carries out squamous subculture, obtain no offspring;Turn to plant in root media without offspring by described, carry out culture of rootage, obtain grape seedling.Take root efficiency high, survival rate of the tissue cultivating and seedling method of the present invention is high, can keep the fine quality of plant.
Description
Technical field
The invention belongs to plant tissue culture seedlings-raising technical field, and in particular to a kind of breeding method of Tissue culture the seedling of grape.
Background technology
The nutritional ingredient grape of grape is not only delicious and tasty, and nutritive value is very high.Grape is containing sugar in ripe berry
Amount is up to 10%-30%, based on glucose.A variety of tartaric acid in grape contribute to digestion, suitably eat a little grapes more, can be good for Pi with
Stomach.Contain mineral calcium, potassium, phosphorus, iron and multivitamin B1, vitamin B2, vitamin B6, vitamin C and dimension in grape
Raw element P etc., the also amino acid containing a variety of needed by human body, often to eat grape are of great advantage to neurasthenia, excessive fatigue.Research hair
Existing, grape can preferably prevent thrombosis than aspirin, and can reduce human serum cholesterol levels, reduce hematoblastic
Prevention cardiovascular and cerebrovascular diseases are had certain effect by cohesiveness.Appropriate fresh grape is eaten daily, can not only reduce angiocardiopathy
Onset risk, it is also particularly beneficial in those ischemic heart diseases and the health of atherosclerotic heart disease patient.Fresh Portugal
Flavonoid substances in grape, energy " cleaning " blood, prevent the formation of cholesterol plaques.Grape is in more black, containing Flavonoid substances
It is more, if but eat Grape Skin and grape pip together, to the protective effect of heart more preferably.
Current grape is mainly using skewer breeding and mound layering mode is inserted, and reproductive efficiency is low.
The content of the invention
It is an object of the invention to overcome the deficiencies of the prior art and provide a kind of breeding method of Tissue culture the seedling of grape.
The present invention is realized in the following way：
A kind of breeding method of Tissue culture the seedling of grape, the liquor potassic permanganate that mass concentration is 1-3% is soaked in by the seed of grape,
It is rinsed afterwards with sterilized water, obtains aseptic seed;The aseptic seed is inoculated into Initial culture base, be just commissioned to train
Support, obtain aseptic seedling;Using the cotyledon of the aseptic seedling and hypocotyl as explant, and it is inoculated in subculture medium, carries out
Squamous subculture, obtains no offspring;Turn to plant in root media without offspring by described, carry out culture of rootage, obtain grape seedling.
It is preferred that, the time being rinsed with the sterilized water is 3-5min.
It is preferred that, the Initial culture base is the g/L of MS+sucrose 15.
It is preferred that, the subculture medium is 0.05 mg/L of MS+6- benzyl aminoadenines+mg/L of methyl α-naphthyl acetate 0.05
The g/L of+sucrose 15.
It is preferred that, the root media is 1/2MS a great number of elements+MS trace element+MS molysite+MS organic matters+naphthalene second
The sour g/L of 2 mg/L+ sucrose 20.
It is preferred that, the condition of the Initial culture is 20-25 DEG C, humidity 60-70%, illumination 2000-5000 lx.
It is preferred that, the condition of the squamous subculture is 20-28 DEG C, humidity 70-80%, illumination 6000-8000 lx.
It is preferred that, the condition of the culture of rootage is 20-30 DEG C of temperature, humidity 80-90%, illumination 10000-15000
lx。
Advantages and positive effects of the present invention：
1st, take root efficiency high, survival rate of tissue cultivating and seedling method of the invention is high, can keep the fine quality of plant.
2nd, the cycle of the invention is short, easy to operate, be adapted to extensive nursery.
3rd, with low cost, market prospects of the invention are good, and economic benefit is good.
Embodiment
Below by embodiment, the invention will be further described, so that advantages and features of the invention are easier to be managed
Solution, but protection scope of the present invention is not limited to these embodiments.
Embodiment 1
A kind of breeding method of Tissue culture the seedling of grape, the liquor potassic permanganate that mass concentration is 1% is soaked in by the seed of grape, it
3min is rinsed with sterilized water afterwards, aseptic seed is obtained;The aseptic seed is inoculated into Initial culture base, Initial culture
Base be the g/L of MS+sucrose 15 carry out Initial culture, 20 DEG C, humidity 60%, illumination 2000lx, obtain aseptic seedling;Will be described sterile
The cotyledon of seedling and hypocotyl are inoculated in subculture medium as explant, and subculture medium is that MS+6- benzyl amino glands are fast
0.05 mg/L of purine+0.05 mg/L of methyl α-naphthyl acetate+sucrose 15 g/L, 25 DEG C, humidity 70%, the lx of illumination 6000, carry out subculture
Culture, obtains no offspring;Turn to plant in root media without offspring by described, root media is that 1/2MS a great number of elements+MS is micro-
Secondary element+MS molysite+MS organic matters+2 mg/L+ sucrose of methyl α-naphthyl acetate 20 g/L, 28 DEG C, humidity 80%, the lx of illumination 10000, carry out
Culture of rootage, obtains grape seedling.
Embodiment 2
A kind of breeding method of Tissue culture the seedling of grape, the liquor potassic permanganate that mass concentration is 3% is soaked in by the seed of grape, it
5min is rinsed with sterilized water afterwards, aseptic seed is obtained;The aseptic seed is inoculated into Initial culture base, Initial culture
Base be the g/L of MS+sucrose 15 carry out Initial culture, 22 DEG C, humidity 70%, the lx of illumination 5000, obtain aseptic seedling;Will be described sterile
The cotyledon of seedling and hypocotyl are inoculated in subculture medium as explant, and subculture medium is that MS+6- benzyl amino glands are fast
0.05 mg/L of purine+0.05 mg/L of methyl α-naphthyl acetate+sucrose 15 g/L, 28 DEG C, humidity 80%, the lx of illumination 8000, carry out subculture
Culture, obtains no offspring;Turn to plant in root media without offspring by described, root media is that 1/2MS a great number of elements+MS is micro-
Secondary element+MS molysite+MS organic matters+2 mg/L+ sucrose of methyl α-naphthyl acetate 20 g/L, 30 DEG C, humidity 90%, the lx of illumination 15000, enter
Row culture of rootage, obtains grape seedling.
Embodiment 3
A kind of breeding method of Tissue culture the seedling of grape, the liquor potassic permanganate that mass concentration is 2% is soaked in by the seed of grape, it
4min is rinsed with sterilized water afterwards, aseptic seed is obtained;The aseptic seed is inoculated into Initial culture base, Initial culture
Base is that the g/L of MS+sucrose 15 carries out 25 DEG C of Initial culture, humidity 70%, the lx of illumination 3000, obtains aseptic seedling;Will be described sterile
The cotyledon of seedling and hypocotyl are inoculated in subculture medium as explant, and subculture medium is that MS+6- benzyl amino glands are fast
0.05 mg/L of purine+0.05 mg/L of methyl α-naphthyl acetate+sucrose 15 g/L, 28 DEG C, humidity 75%, the lx of illumination 7000, carry out subculture
Culture, obtains no offspring;Turn to plant in root media without offspring by described, root media is that 1/2MS a great number of elements+MS is micro-
Secondary element+MS molysite+MS organic matters+2 mg/L+ sucrose of methyl α-naphthyl acetate 20 g/L, 28 DEG C, humidity 85%, the lx of illumination 12000, carry out
Culture of rootage, obtains grape seedling.
Embodiment 4
A kind of breeding method of Tissue culture the seedling of grape, the liquor potassic permanganate that mass concentration is 3% is soaked in by the seed of grape, it
5min is rinsed with sterilized water afterwards, aseptic seed is obtained;The aseptic seed is inoculated into Initial culture base, Initial culture
Base be the g/L of MS+sucrose 15 carry out Initial culture, 25 DEG C, humidity 60%, the lx of illumination 2000, obtain aseptic seedling;By the nothing
The cotyledon of vaccine and hypocotyl are inoculated in subculture medium as explant, and subculture medium is MS+6- benzyl amino glands
0.05 mg/L of purine+0.05 mg/L of methyl α-naphthyl acetate+sucrose 15 g/L, 28 DEG C, humidity 70%, the lx of illumination 8000, carry out after
It is commissioned to train foster, obtains no offspring;Turn to plant in root media without offspring by described, root media is 1/2MS a great number of elements+MS
Trace element+MS molysite+MS organic matters+2 mg/L+ sucrose of methyl α-naphthyl acetate 20 g/L, 30 DEG C, humidity 80%, the lx of illumination 15000,
Culture of rootage is carried out, grape seedling is obtained.
Claims (8)
1. a kind of breeding method of Tissue culture the seedling of grape, it is characterised in that it is 1-3%'s that the seed of grape is soaked in into mass concentration
Liquor potassic permanganate, is rinsed with sterilized water afterwards, obtains aseptic seed;The aseptic seed is inoculated into Initial culture base
In, Initial culture is carried out, aseptic seedling is obtained;Using the cotyledon of the aseptic seedling and hypocotyl as explant, and it is inoculated in subculture
In culture medium, squamous subculture is carried out, no offspring is obtained;Turn to plant in root media without offspring by described, carry out culture of rootage,
Obtain grape seedling.
2. the breeding method of Tissue culture the seedling of grape according to claim 1, it is characterised in that be rinsed with the sterilized water
Time be 3-5min.
3. the breeding method of Tissue culture the seedling of grape according to claim 1, it is characterised in that the Initial culture base is MS
The g/L of+sucrose 15.
4. the breeding method of Tissue culture the seedling of grape according to claim 1, it is characterised in that the subculture medium is MS
The 0.05 mg/L of+6- benzyl aminoadenines+g/L of 0.05 mg/L of methyl α-naphthyl acetate+sucrose 15.
5. the breeding method of Tissue culture the seedling of grape according to claim 1, it is characterised in that the root media is 1/
2MS a great number of elements+MS trace element+MS molysite+MS organic matters+the g/L of 2 mg/L+ sucrose of methyl α-naphthyl acetate 20.
6. the breeding method of Tissue culture the seedling of grape according to claim 1, it is characterised in that the condition of the Initial culture is
20-25 DEG C, humidity 60-70%, illumination 2000-5000 lx.
7. the breeding method of Tissue culture the seedling of grape according to claim 1, it is characterised in that the condition of the squamous subculture
For 20-28 DEG C, humidity 70-80%, illumination 6000-8000 lx.
8. the breeding method of Tissue culture the seedling of grape according to claim 1, it is characterised in that the condition of the culture of rootage
For 20-30 DEG C of temperature, humidity 80-90%, illumination 10000-15000 lx.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710297132.9A CN106982736A (en) | 2017-04-28 | 2017-04-28 | A kind of breeding method of Tissue culture the seedling of grape |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710297132.9A CN106982736A (en) | 2017-04-28 | 2017-04-28 | A kind of breeding method of Tissue culture the seedling of grape |
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| CN106982736A true CN106982736A (en) | 2017-07-28 |
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| CN201710297132.9A Pending CN106982736A (en) | 2017-04-28 | 2017-04-28 | A kind of breeding method of Tissue culture the seedling of grape |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101564011A (en) * | 2009-05-18 | 2009-10-28 | 杭州蓝天园林建设集团有限公司余杭分公司 | High-efficient breeding technique of Eurasian raisin grape |
| CN102144547A (en) * | 2011-01-14 | 2011-08-10 | 四川农业大学 | Method for quickly breeding and transplanting grape stock unit |
-
2017
- 2017-04-28 CN CN201710297132.9A patent/CN106982736A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101564011A (en) * | 2009-05-18 | 2009-10-28 | 杭州蓝天园林建设集团有限公司余杭分公司 | High-efficient breeding technique of Eurasian raisin grape |
| CN102144547A (en) * | 2011-01-14 | 2011-08-10 | 四川农业大学 | Method for quickly breeding and transplanting grape stock unit |
Non-Patent Citations (5)
| Title |
|---|
| MARCELA VILAPLANA ET AL.: "Regeneration of Grapevines (Vitis spp.) in vitro: Formation of dventitious Buds on Hypocotyls and Cotyledons of Somatic Embryos", 《JOURNAL OF PLANT PHYSIOLOGY》 * |
| 傅润民: "《果树瓜类生物工程育种》", 31 May 1994, 农业出版社 * |
| 宗桦等: "葡萄离体培养无菌苗体系技术研究", 《江苏农业科学》 * |
| 张远记等: "早熟葡萄种子培养和胚培养", 《西北农业大学学报》 * |
| 王蒂等: "《植物组织培养》", 31 August 2013, 中国农业出版社 * |
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Application publication date: 20170728 |