CN106978461A - A kind of method that solid slag prepares functional activity peptide after glutamic acid fermentation - Google Patents
A kind of method that solid slag prepares functional activity peptide after glutamic acid fermentation Download PDFInfo
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Abstract
A kind of method that solid slag prepares functional activity peptide after glutamic acid fermentation, it is characterised in that comprise the following steps：(1) raw material selection and pre-treatment；(2) crush and separated with screenings；(3) using chemically and physically impurity of the method combining form in addition to deproteinized；(4) two albuminoid enzyme directionally hydrolyzings react；(5) it is drying to obtain finished activated peptide after the concentration of enzymolysis material.Bioactive peptide molecule amount is distributed in 500~1500Da ratio ＞ 60%, and the antioxidation activity that the product is obtained by determining free radical (DPPH) ability of removing is 50%~70%.The product can be used in food as nutrient protein replenishers or play antioxidation, can be also used in organic fertilizer directly absorbing for plant or with reference to some metallic elements in soil as organic nitrogen source, improves soil quality.
Description
Technical field
The present invention relates to food processing technology field, functional activity is prepared more particularly, to solid slag after a kind of glutamic acid fermentation
The method of peptide.
Background technology
Glutamic acid fermentation bacteria residue is the principal by product of monosodium glutamate industry, and annual output is up to tons up to a million.These paddy of past
Propylhomoserin ferments bacteria residue mainly by discharge of wastewater, had not only caused environmental pollution but also had wasted resource.It is comprehensive to monosodium glutamate industry byproduct
Run hair jointly, the comprehensive utilization of grain resource is realized, with important practical significance.According to the evaluation criterion of biology, production
The bacillus of monosodium glutamate and all biosynthetic products are all harmless.It is a kind of renewable resource underused, at present
Only sold in China's most enterprises as feed.
Protease is divided into restriction endonuclease and end enzyme cutting, and restriction endonuclease type has acid protease, neutral proteinase, and (hay bacillus sends out
Ferment is originated and aspergillus oryzae fermentable sources), bromelain, it be inside the protein molecular decompose, can be in a short time
High molecular weight protein is degraded to peptides, the enzyme preparation containing sulfhydryl protected material (such as：Cysteine hydrochloride) feelings that exist
Under condition, enzymolysis vigor is improved；End enzyme cutting type has papain, alkali protease, trypsase, it be from the N- of albumen/
C- ends by amino acid or small hydrolase polypeptide or it is single-minded for some amino acid start hydrolysis, can be by the big peptides water of molecular weight
Solve as the small peptides of molecular weight or amino acid.
It is the method that rice prepares peptide product that Application No. CN200810023519 patent document, which provides a kind of raw material,
But its alkali soluble acid first is heavy (pH12), to being had a strong impact on starch structure and recovery yield, secondly, albumen is in strong basicity bar
Under part, easily reacted with alkali, form carcinogen (thering is document to look into), although obtained albumen part has anti-oxidant work
Property, but also there is the risk of carcinogen；Moreover, after alkali soluble acid is heavy, then isoelectric point form, it is clear that yield it is most
50%, will not be too high, integrated artistic does not maximally utilize protein resource in rice, and still part is discarded.
Application No. CN201510123351.6 patent document obtains many there is provided a kind of marine alga by microbial degradation
The method of the materials such as peptide.However, the fermentation step of the technology is for preparing active peptide, rather than to remaining solid slag after fermentation
The processing of progress.
Application No. CN201610365195.9 patent document provides the method that polypeptide is obtained using proteolysis.So
And, its enzymatic hydrolysis condition is alkaline pH 9.5, and albumen is possible to react generation carcinogen with alkali, changes protein normal
Functional characteristic, using sesame seed meal, that is, squeezing the dregs of rice after sesame, to contain oil plant composition more, and this is not considered in documents
Point, oil plant composition is dried to obtain as active peptide exists in supernatant jointly, and one side grease is that unsaturated composition is more, influence production eventually
The storage life of product, the presence of another aspect grease, the purity of active peptide is not high.
The content of the invention
In view of the above-mentioned problems existing in the prior art, function is prepared this application provides solid slag after a kind of glutamic acid fermentation to live
The method of property peptide.The present invention is active peptide obtained to be can be used in food as nutrient protein replenishers or plays antioxidation,
It can be also used for directly absorbing in organic fertilizer or with reference to some metallic elements in soil, changing for plant as organic nitrogen source
Kind soil quality.
Technical scheme is as follows：
A kind of method that solid slag prepares functional activity peptide after glutamic acid fermentation, comprises the following steps：
(1) raw material selection and pre-treatment：Selection is not gone mouldy, the smelly and obvious qualitative change of acid, by glutamic acid fermentation technique
Solid slag after processing, removes obvious inorganic impurity and organic impurities；
(2) crush：Solid slag after pre-treatment is realized that screenings is separated after coarse crushing, finely divided, screening；
(3) removal of impurities：It is that 15%~25%, pH is that 6.5~8.5, temperature of charge is 50 DEG C~75 DEG C to adjust concentration of slurry, is mixed
The surfactant-based chemical substance of conjunction in terms of dry 0.1 ‰~0.5 ‰, stirring reaction 1h~5h passes through eddy flow washing side
Formula goes the removal of impurity, collects eddy flow washing heavy phase；
(4) enzyme digestion reaction：Eddy flow washing heavy phase material by sizing mixing, heat exchange processing after, first add protease A in peptide
After enzyme, stirring reaction, then Cathepsin B peptide ending enzyme is added, continue to react；Concentration of sizing mixing is 15%~25%, and temperature of charge is 45
DEG C~60 DEG C, two kinds of enzyme preparation total reaction times are 0.5h~8h, and endopeptidase and peptide ending enzyme adding proportion are 1:2~2:1, always add
Dosage is 0.3 ‰~0.8 ‰, and whole slurry pH is controlled 4~8；Addition protease A and Cathepsin B total solid slag after reacting respectively
The ratio for being converted into soluble component is at least 50% and 70% respectively.
(5) concentration is with drying：It is 15%~30% that above-mentioned enzymolysis liquid is concentrated into concentration, then using spray dried form
It is to obtain finished product to dry materials.
Step (1) described inorganic impurity includes soil, sandstone, cinder, brick and tile, glass fragment, metal object, described organic miscellaneous
Matter includes plant roots, stem, hair, seed, xenogenesis grain, worm pupa, worm corpse.
It is preferred that, after screening after step (2) coarse crushing and the crushing of finely divided two step, the sieve mesh number of screening is 60~80
Mesh, the oversize of finely divided rear screening is pumped into coarse crushing charging aperture by the pipeline of collection port and crushed simultaneously with new material, finally
Control oversize in fiber substance content ＞ 60%, by the fineness of materials after screening be 300 mesh percent of pass reach 98% with
On.
It is preferred that, the impurity that step (3) is removed is lipid material, soluble ash content or other water-soluble substanceses；The table
Face activating agent class chemical substance includes Emulsifier LT-60M, sorbitol anhydride kind polyester, diacetyl tartarate
Single double glyceride, sucrose fatty ester, dodecyl sodium sulfate, polyglyceryl fatty acid ester, disodium ethylene diamine tetraacetate, when using
Select one of which or several additions simultaneously.
It is preferred that, the Emulsifier LT-60M includes TWEEN Series, the sorbitol anhydride kind polyester
Including Span series.
It is preferred that, step (4) described endopeptidase include acid protease, neutral proteinase, one kind of bromelain or
Person is several, and the peptide ending enzyme includes papain, alkali protease, the one or several kinds of trypsase.
It is preferred that, the neutral proteinase is that fermenting bacillus subtilis is originated or aspergillus oryzae fermentable sources.
It is preferred that, while protease A is added, cysteine hydrochloride 0.01 ‰~0.05 ‰ are added, collaboration promotes
Protease A hydrolysis.
It is preferred that, the bioactive peptide molecule amount prepared is distributed in 500~1500Da ratio ＞ 60%, clear by determining
Except the antioxidation activity that free radical DPPH abilities obtain the product is 50%~70%.
The applicant additionally provides a kind of application for the active peptide that described method is prepared, for being used as egg in food
White nutritious supplementary pharmaceutical plays antioxidation, or for directly being absorbed for plant as organic nitrogen source in organic fertilizer
Or some metallic elements in soil are combined, improve soil quality.
Wherein, the solid slag after the glutamic acid fermentation PROCESS FOR TREATMENT described in step (1) includes three major types：The first kind is oiliness thing
Material residue, the raw material is to separate the dregs of rice after oil plant again after glutamic acid fermentation PROCESS FOR TREATMENT, including sesame seed meal, sunflower seed dregs, flower
The raw dregs of rice, the flaxseed dregs of rice, cotton rapeseed meal, rapeseed dregs, dregs of beans etc., physical and chemical index are protein content >=40%, fat content≤20%；The
Two classes are the slag after Cereals class is processed, the raw material be after conventional deep processing processing again after glutamic acid fermentation PROCESS FOR TREATMENT
Slag, including maize pulp, rice residue, vinasse, wheat class slag etc., physical and chemical index is protein content >=45%, fat content≤12%；The
Three classes are the slag after potato class is processed, the raw material be after conventional deep processing processing again after glutamic acid fermentation PROCESS FOR TREATMENT
Slag, including potato residues, sweet potato waste, manioc waste etc., physical and chemical index is protein content >=40%, fat content≤8%.
The present invention is beneficial to be had technical effect that：
Solid slag after present invention selection glutamic acid fermentation prepares active peptide, is, because it has certain protein content, to pass through
Solid slag after fermentation, fermentative microorganism is directed in each corner to whole material, just as loosening the soil earthworm so that solid slag
Structure is compared to unfermentable loose, and many materials are converted or degraded by Institute of Micro-biology, are added albumen and are separated with other compositions
Difficulty.This patent fully realizes the difficulty of separation, in order to obtain the active peptide of high-quality, high-purity, takes first a series of
Measure non-protein composition is efficiently separated, high molecular weight protein is then decomposed into small active peptides by enzymolysis means again.
Application No. CN201510240153 patent document and similitude of the present invention are it is all to integrate discarded solid slag
Utilize, lifting value, the problem of solving discarded solid slag jointly.However, having essential distinction on two kinds of technical steps：(1) raw material group
Into upper difference, what the present invention mainly collected is rich protein-contg plant protein material, and documents are organic waste, wherein constituting
Not frozen composition；(2) in handling process, documents use strain fermentation form, obtain bacterium powder, and the present invention is fully combined
Raw material composition and property, is handled by special process, obtains accordingly optimizing product.
The present invention considers composition composition main in discarded solid slag, and fully takes into account before finished product is obtained removal of impurities
Matter, is adjusted, chemical substance, three kinds of mode collective effects of washing using pH value, removes impurity, and on the influence of the property of albumen compared with
It is small.Although the present invention is best suitable for pH in alkaline case there is also enzyme preparation, pH value can be controlled below 8, it is ensured that albumen
Security.
The present invention uses the other enzyme segmenting function of two species, and endopeptidase is fixed point or random cutting inside protein molecular,
The smaller albumen of decomposition texture；Peptide ending enzyme is cutting or specific recognition some amino acid point cutting since C-/N- ends,
Molecular structure is further reduced to soluble peptides or amino acid.
The present invention is in order that protease A plays better effect, and the action site of protease A is the key containing sulfydryl,
But need to sulfhydryl protected, the effect of protease just can be more efficient, and protection of the cysteine hydrochloride to sulfydryl is made
With obvious.
Routine hydrolysis albumen slag is generally up to 50% for the ratio of solvable peptide, and high conversion rate of the present invention, and its reason has：
(1) non-protein composition is efficiently separated first, high degree improves the concentration of substrate of late protein enzyme effect；
The other enzyme combination application of (2) two species, and foreign aid's auxiliary agent is taken, enzyme preparation effect is performed into maximum.
Solid slag had not only caused environmental pollution but also had wasted resource mainly by discharge of wastewater after conventional glutamic acid fermentation.This
Technology is comprehensively utilized to industrial waste solid slag, and the active peptide product with functional activity is prepared after despumation, grain is realized
The comprehensive utilization of resource is eaten, with important practical significance and application value.
Embodiment
With reference to embodiment, the present invention is specifically described.
Embodiment 1
(1) raw material selection and pre-treatment：Selection is not gone mouldy, the smelly and obvious qualitative change of acid, by glutamic acid fermentation technique
Oiliness material slag after processing, removes obvious inorganic impurity and organic impurities, and inorganic impurity includes soil, sandstone, cinder, brick
Watt, glass fragment, metal object and other inorganic substances, organic impurities includes plant roots, stem, hair, seed, xenogenesis grain, worm pupa, worm
Corpse.
(2) crush：By the solid slag after pre-treatment after coarse crushing and finely divided two step are crushed after screening, the sieve of screening
Mesh number is 60 mesh, and when the oversize of screening after finely divided runs up to a certain amount of, equipment is automatically by thing in oversize temporary storage tank
Material is pumped into coarse crushing charging aperture and crushed simultaneously with new material, fiber substance content 62% in oversize is determined, after screening
Fineness of materials be that 300 mesh percent of pass are 98%.
(3) removal of impurities：It is that 15%, pH is that 8.5, temperature of charge is 50 DEG C to adjust concentration of slurry, mixing in terms of dry 0.5 ‰
Surfactant-based chemical substance, the chemical substance of addition is Emulsifier LT-60M and dodecyl sulphur
Sour sodium is added simultaneously, and adding proportion is 4:1, stirring reaction 5h, the removal of impurity is gone by eddy flow mode of washing, collects eddy flow washing weight
Phase.Such combination removal lipid material scope is wider, and the HLB value that can remove amphipathic property qualitative correlation is 4~15 lipid
Material, soluble ash content or other water-soluble substanceses.
(4) enzyme digestion reaction：The heavy phase material of eddy flow washing, sizes mixing as 15%, and heat exchange to temperature of charge is 60 DEG C, adjusts pH
Value 4, first adding protease A endopeptidase, (addition acid protease and neutral proteinase, adding proportion is 1:1, wherein neutral protein
Enzyme refers in particular to fermenting bacillus subtilis), it is synchronous to add after cysteine hydrochloride 0.05 ‰, stirring reaction, then add Cathepsin B end peptide
Enzyme papain, continues to react, and two kinds of enzyme preparation total reaction times are 0.5h, and total addition level is 0.8 ‰ (endopeptidase and end peptides
Enzyme addition ratio is 2:1).Determine the ratio that total solid slag after protease A and Cathepsin B react respectively is converted into soluble component
Respectively 67% and 81%.
(5) concentration is with drying：It is 15% that above-mentioned enzymolysis liquid is concentrated into concentration, then using spray dried form to material
Drying to obtain finished product.
Embodiment 2
(1) raw material selection and pre-treatment：Selection is not gone mouldy, the smelly and obvious qualitative change of acid, by glutamic acid fermentation technique
The slag after potato class processing after processing, removes obvious inorganic impurity and organic impurities, inorganic impurity include soil, sandstone, cinder,
Brick and tile, glass fragment, metal object and other inorganic substances, organic impurities include plant roots, stem, hair, seed, xenogenesis grain, worm pupa,
Worm corpse.
(2) crush：By the solid slag after pre-treatment after coarse crushing and finely divided two step are crushed after screening, the sieve of screening
Mesh number is 80 mesh, and when the oversize of screening after finely divided runs up to a certain amount of, equipment is automatically by thing in oversize temporary storage tank
Material is pumped into coarse crushing charging aperture and crushed simultaneously with new material, fiber substance content 71% in detection oversize, after screening
Fineness of materials be that 300 mesh percent of pass are 99%.
(3) removal of impurities：It is that 25%, pH is that 6.5, temperature of charge is 75 DEG C to adjust concentration of slurry, mixing in terms of dry 0.1 ‰
Surfactant-based chemical substance, the chemical substance of addition is diacetyl tartarate list double glyceride and dodecyl sodium sulfate
Collocation addition, adding proportion is 3:2, stirring reaction 1h, the removal of impurity is gone by eddy flow mode of washing, collects eddy flow washing heavy phase.
The impurity of removing is lipid material, soluble ash content or other water-soluble substanceses that hydrophilic and oleophilic property HLB numerical value is 1~16.
(4) enzyme digestion reaction：The heavy phase material of eddy flow washing, sizes mixing as 25%, and heat exchange to temperature of charge is 45 DEG C, adjusts pH
Value 8, first adding protease A endopeptidase, (acid protease and bromelain, adding proportion are 1:1) synchronous addition cysteine
After hydrochloride 0.01 ‰, stirring reaction, then add Cathepsin B peptide ending enzyme (addition alkali protease and trypsase, adding proportion
For 2:1), continue to react, two kinds of enzyme preparation total reaction times are 8h, total addition level is 0.3 ‰ (endopeptidase and peptide ending enzyme additions
Ratio is 1:2).Determining the ratio that total solid slag is converted into soluble component after protease A and Cathepsin B react respectively is respectively
51% and 73%.
(5) concentration is with drying：It is 30% that above-mentioned enzymolysis liquid is concentrated into concentration, then using spray dried form to material
Drying to obtain finished product.
Embodiment 3
(1) raw material selection and pre-treatment：Selection is not gone mouldy, the smelly and obvious qualitative change of acid, by glutamic acid fermentation technique
The slag after the processing of Cereals class after processing, removes obvious inorganic impurity and organic impurities, inorganic impurity includes soil, sandstone, coal
Slag, brick and tile, glass fragment, metal object and other inorganic substances, organic impurities include plant roots, stem, hair, seed, xenogenesis grain, worm
Pupa, worm corpse.
(2) crush：By the solid slag after pre-treatment after coarse crushing and finely divided two step are crushed after screening, the sieve of screening
Mesh number is 70 mesh, and when the oversize of screening after finely divided runs up to a certain amount of, equipment is automatically by thing in oversize temporary storage tank
Material is pumped into coarse crushing charging aperture and crushed simultaneously with new material, fiber substance content 65% in detection oversize, after screening
Fineness of materials be that 300 mesh percent of pass are 99%.
(3) removal of impurities：It is that 20%, pH is that 7.0, temperature of charge is 62 DEG C to adjust concentration of slurry, mixing in terms of dry 0.3 ‰
Surfactant-based chemical substance, the chemical substance of addition is that polyglyceryl fatty acid ester and disodium ethylene diamine tetraacetate add simultaneously
Plus, compound proportion is 1:1, stirring reaction 3h, the removal of impurity is gone by eddy flow mode of washing, collects eddy flow washing heavy phase.Remove
Impurity is lipid material, soluble ash content or other water-soluble substanceses that hydrophilic and oleophilic property HLB is 1~20.
(4) enzyme digestion reaction：The heavy phase material of eddy flow washing, sizes mixing as 20%, and heat exchange to temperature of charge is 50 DEG C, adjusts pH
Value 6.5, first adding protease A endopeptidase, (addition neutral proteinase and bromelain, adding proportion is 1:1, wherein neutral egg
White enzyme refers in particular to aspergillus oryzae fermentable sources), it is synchronous to add after cysteine hydrochloride 0.03 ‰, stirring reaction, then add Cathepsin B
Peptide ending enzyme trypsase, continues to react, and two kinds of enzyme preparation total reaction times are 4h, and total addition level is 0.5 ‰ (endopeptidase and end peptides
Enzyme addition ratio is 1:1).Determine the ratio that total solid slag after protease A and Cathepsin B react respectively is converted into soluble component
Respectively 57% and 76%.
(5) concentration is with drying：It is 22% that above-mentioned enzymolysis liquid is concentrated into concentration, then using spray dried form to material
Drying to obtain finished product.
Embodiment 4
Each step is all identical with embodiment 1, and the chemical classes only added in removal step are different with proportioning, are
Sorbitol anhydride kind polyester and disodium ethylene diamine tetraacetate compounding use, adding proportion is 3:1.
Embodiment 5
Each step is all identical with embodiment 2, and the chemical classes only added in removal step are different with proportioning, are
Sucrose fatty ester and disodium ethylene diamine tetraacetate compounding use, adding proportion is 3:2.
Embodiment 6
Each step is all identical with embodiment 3, and the chemical classes only added in removal step are different with proportioning, are
Polyglyceryl fatty acid ester and dodecyl sodium sulfate compounding use, adding proportion is 1:1.
Test case 1
The active peptide physical and chemical composition compounded the analysis prepared to embodiment 1~3, test result is as shown in table 1.
The active peptide product physical and chemical composition of table 1 is determined
| Moisture/% | Albumen/% | Fat/% | |
| Embodiment 1 | 4.9 | 82 | 2.6 |
| Embodiment 2 | 4.4 | 75 | 0.8 |
| Embodiment 3 | 4.5 | 78 | 1.5 |
Test case 2
The active peptide product molecular weight distribution situation test result such as institute of table 2 compounded prepared to embodiment 1~3
Show.
The active peptide product molecular weight distribution area situation of table 2
| ＜ 500Da | 500~1500Da | ＞ 1500Da | |
| Embodiment 1 | 19% | 68% | 13% |
| Embodiment 2 | 23% | 65% | 12% |
| Embodiment 3 | 18% | 70% | 12% |
Test case 3
The active peptide product compounded prepared to embodiment 1~3, catches kit measurement with free radical DPPH and lives
The oxidation resistance of property peptide, test result is as shown in table 3.
The active peptide product antioxidation activity of table 3
| Embodiment 1 | Embodiment 2 | Embodiment 3 | |
| Antioxidation activity | 59% | 54% | 65% |
Test case 4
The active peptide product compounded prepared to embodiment 3, is mixed with the soil rich in heavy metal, and conduct has
Machine nitrogen source compounding supplies plant growth into organic fertilizer, and heavy metal in soil changes of contents is as shown in table 4 before and after culture.
Table 4 cultivates front and rear heavy metal in soil content detection table
The active peptide that the present invention is obtained, compared with prior art, main is the ratio height that albumen slag is converted into solvable peptide,
Obtained albumen is attributed to vegetable protein peptides, and the complete molten peptide product of current vegetable protein is few, and the patent enriches product preparation
The ratio ＞ 60% of technology, protein content ＞ 70%, and 500~1500Da contents, these properties it is similar to existing product or
The product produced better than prior art.
Specific implementation case described herein is only illustrated as to spirit of the invention and part Experiment.The present invention
The technical staff in the field can make various modifications or supplement to described specific implementation case or use class
As mode substitute, but spirit without departing from the present invention or surmount scope defined in appended claims.
Claims (10)
1. a kind of method that solid slag prepares functional activity peptide after glutamic acid fermentation, it is characterised in that comprise the following steps：
(1) raw material selection and pre-treatment：Selection is not gone mouldy, the smelly and obvious qualitative change of acid, by glutamic acid fermentation PROCESS FOR TREATMENT
Solid slag afterwards, removes obvious inorganic impurity and organic impurities；
(2) crush：Solid slag after pre-treatment is realized that screenings is separated after coarse crushing, finely divided, screening；
(3) removal of impurities：Adjust concentration of slurry be that 15%~25%, pH is that 6.5~8.5, temperature of charge is 50 DEG C~75 DEG C, mixing with
The surfactant-based chemical substance of dry meter 0.1 ‰~0.5 ‰, stirring reaction 1h~5h, is gone by eddy flow mode of washing
The removal of impurity, collects eddy flow washing heavy phase；
(4) enzyme digestion reaction：Eddy flow washing heavy phase material by sizing mixing, heat exchange processing after, first add protease A endopeptidase, stir
Mix after reaction, then add Cathepsin B peptide ending enzyme, continue to react；Concentration of sizing mixing is 15%~25%, and temperature of charge is 45 DEG C~60
DEG C, two kinds of enzyme preparation total reaction times are 0.5h~8h, and endopeptidase and peptide ending enzyme adding proportion are 1:2~2:1, total addition level is
0.3 ‰~0.8 ‰, whole slurry pH are controlled 4~8；Addition protease A and Cathepsin B after reacting respectively total solid slag be converted into
The ratio of soluble component is at least 50% and 70% respectively.
(5) concentration is with drying：It is 15%~30% that above-mentioned enzymolysis liquid is concentrated into concentration, then using spray dried form to thing
Expect drying to obtain finished product.
2. according to the method described in claim 1, it is characterised in that step (1) described inorganic impurity includes soil, sandstone, coal
Slag, brick and tile, glass fragment, metal object, the organic impurities include plant roots, stem, hair, seed, xenogenesis grain, worm pupa, worm corpse.
3. according to the method described in claim 1, it is characterised in that step (2) coarse crushing and finely divided two step crush after after
Screening, the sieve mesh number of screening is 60~80 mesh, and the oversize of finely divided rear screening is pumped into coarse crushing by the pipeline of collection port
Charging aperture is crushed simultaneously with new material, fiber substance content ＞ 60% in final control oversize, passes through the material after screening
Fineness is that 300 mesh percent of pass reach more than 98%.
4. according to the method described in claim 1, it is characterised in that the impurity that step (3) is removed is lipid material, soluble ash
Divide or other water-soluble substanceses；The surfactant-based chemical substance includes Emulsifier LT-60M, mountain
Pears alcohol anhydride polyester, diacetyl tartarate list double glyceride, sucrose fatty ester, dodecyl sodium sulfate, polyglycerol fatty acid
Ester, disodium ethylene diamine tetraacetate, select one of which or several additions simultaneously when using.
5. method according to claim 4, it is characterised in that the Emulsifier LT-60M includes telling
Warm series, the sorbitol anhydride kind polyester includes Span series.
6. according to the method described in claim 1, it is characterised in that step (4) described endopeptidase includes acid protease, neutrality
The one or several kinds of protease, bromelain, the peptide ending enzyme includes papain, alkali protease, trypsase
One or several kinds.
7. method according to claim 5, it is characterised in that the neutral proteinase is that fermenting bacillus subtilis is originated or rice
Aspergillus fermentable sources.
8. according to the method described in claim 1, it is characterised in that while protease A is added, add cysteine hydrochloric acid
Salt 0.01 ‰~0.05 ‰, collaboration promotes protease A hydrolysis.
9. method according to claims 1 to 9, it is characterised in that the bioactive peptide molecule amount prepared is distributed in 500~
1500Da ratio ＞ 60%, by determine remove free radical DPPH abilities obtain the antioxidation activity of the product for 50%~
70%.
10. the application for the active peptide that the method described in claim 10 is prepared, it is characterised in that for being used as egg in food
White nutritious supplementary pharmaceutical plays antioxidation, or for directly being absorbed for plant as organic nitrogen source in organic fertilizer
Or some metallic elements in soil are combined, improve soil quality.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108929331A (en) * | 2018-08-21 | 2018-12-04 | 山东齐发药业有限公司 | The preparation method of rumensin, rumensin and products thereof |
| CN109251097A (en) * | 2018-10-26 | 2019-01-22 | 湖北源清生物科技股份有限公司 | A kind of biology compound organic fertilizer |
| CN109265246A (en) * | 2018-10-26 | 2019-01-25 | 湖北源清生物科技股份有限公司 | A kind of disease resistance biological organic fertilizer |
| CN109485499A (en) * | 2018-12-03 | 2019-03-19 | 上海农乐生物制品股份有限公司 | A kind of preparation method of low lead organic slow-release plant nitrogen fertilizer |
| CN109601693A (en) * | 2018-12-03 | 2019-04-12 | 无锡金农生物科技有限公司 | A kind of high-purity rice protein powder and the preparation method and application thereof |
| CN114051533A (en) * | 2019-07-02 | 2022-02-15 | 天野酶制品株式会社 | Method for producing cysteine from glutathione |
| CN111635766A (en) * | 2020-06-11 | 2020-09-08 | 中国科学院沈阳应用生态研究所 | Method for acidifying soil conditioner by using vinasse production facility and application |
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