CN106962605A - A kind of plant-based animal immune potentiator and its preparation method and application - Google Patents
A kind of plant-based animal immune potentiator and its preparation method and application Download PDFInfo
- Publication number
- CN106962605A CN106962605A CN201710216799.1A CN201710216799A CN106962605A CN 106962605 A CN106962605 A CN 106962605A CN 201710216799 A CN201710216799 A CN 201710216799A CN 106962605 A CN106962605 A CN 106962605A
- Authority
- CN
- China
- Prior art keywords
- fermentation
- ginseng
- plant
- cordyceps
- fermented
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C9/00—Fertilisers containing urea or urea compounds
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Pest Control & Pesticides (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
A kind of plant-based animal immune potentiator and its preparation method and application, by cordyceps（Cordyceps militaris）It is inoculated with the solid medium based on ginseng or the ginseng dregs of a decoction, fermented, sterilization processing and the product obtained.The immunoenhancement result of the present invention is obvious.Experiment is proved, after two kinds of tunnings are fed through spice, and suppressed phagocyte and lymphocyte function can be made to recover normal, and strengthens cellular immunity function, promotes the breeding of humoral immunity cell, ripe and antibody is produced, and greatly improves the immune effect of vaccine.
Description
Technical field
The invention belongs to animal health-care product field, and in particular to a kind of plant-based animal immune potentiator and its preparation side
Method and application.
Background technology
In recent years, China's fermentation pharmacy industry is developed rapidly, and medicinal fungi bi-directional fermentation technology is current exploitation Chinese medicine
Most promising approach, medicinal fungi fermentation technique refers to using medicinal fungi as fermented bacterium, by the use of Chinese medicine as property of medicine matrix,
Both collectively form fementative composition, and both sides are adapted and carry out the technology of solid fermentation under certain condition, mix Chinese medicine conduct
Property of medicine substrate fermentation, make the immunoenhancement result of tunning has apparent raising than alone any effect therein.Through
Experiment, the product fermented by fermentation substrate of ginseng through cordyceps has after being fed through spice to Immune Function In Animals to be repaiied more by force
Multiple and humidification, greatly strengthen resistance of the animal to disease.
China's medicinal fungi species is enriched, and application disease is wide, has been directed to each important sick section of hospital each relevant with human body
System.Many kinds have the effects of regulation body's immunity, to have studied in terms of anticancer, anti-inflammatory more, closely over several years
Antivirus action, immunological enhancement are received much concern, medicinal fungi product has been related to SARS, AIDS, avian influenza virus
It is exciting Deng effective report.Cordyceps are long in the research history of China, and Cordyceps militaris is Ascomycotina, ergot
Mesh, Clavicipitaceae, the type sepecies of Cordyceps, scientific name is Cordyceps militaris, also known as northern Chinese caterpillar Fungus, Cordceps militaris etc.,
The traditional Chinese medical science thinks that cordyceps sinensis enters lung kidney two warp, can tonifying lung it is cloudy, and can kidney-replenishing, cure mainly kidney deficiency, impotence and seminal emission, soreness of waist and knee joint, after being ill
Weakness, chronic cough is weak, phthisical cough phlegm blood, spontaneous sweating etc., be it is unique it is a kind of can be while balancing, adjusting the Chinese medicine of negative and positive.
Modern science, which proves Cordyceps militaris, not only has special nutritive value, and has obvious medical value.It is wherein outstanding
It is the most notable with the medical value of the various bioactivators such as cordycepic acid, cordycepin, Cordyceps sinensis polysaccharide and SOD.Cordycepic acid (sweet dew
Alcohol) cranium pressure can be significantly decreased, promote body metabolism, thus cerebral hemorrhage and cerebral thrombosis disease is eased.Cordyceps sinensis
Element is a kind of nucleosides material with antibacterial activity, there is very strong inhibitory action to nuclear polyadenylic acid polymerase.Turn in DNA
Make mRNA dysmaturities during record mRNA, suppress the growth of cancer cell.And play the role of hypoglycemic.Cordyceps sinensis polysaccharide is a kind of high
The galactomannans of branch is spent, it can promote lymphocyte transformation, improve the antibody content of serum IgG and the immune work(of body
Can, enhancing body itself anticancer presses down the ability of cancer.SOD (superoxide dismutase) can eliminate superoxide radical in body, tool
There are anti-aging, anticancer suppression cancer.In addition, Cordyceps militaris is also containing abundant selenium (Se), this trace element is by big the father-in-law
It is the essential trace elements of the human body to recognize, and is the activated centre of glutathione peroxidase, is connected in the form of selenocystein
On the peptide chain of zymoprotein, the normal permeability of the stability of cell membrane is protected, and stimulates the generation of immunoglobulin and antibody,
Strengthen immunity of organism and oxidation resistance.Meanwhile, substantial amounts of scientific practice proves that selenium can significantly suppress the growth of cancer cell.
Wherein, the immunoregulation effect of Cordyceps militaris is especially protruded, by carrying out lymph conversion test, Ea and E roses to mouse
Rare flower experiment, specific immunity Rosette Test (Macrophage inflammatory protein-1α, the experiment of anti-sheep red blood cell (SRBC) antibody titer), as a result
Show that Polysaccharides in Cultured Cordyceps militaris has the immunity function that is significantly improved.The present invention be on the basis of the fermentation activity of cordyceps,
Using solid-fermented technique, using ginseng as culture medium, the significant plant-based animal immunopotentiator of effect of fermenting, both at home and abroad
Still belong to the first.
Meanwhile, with developing rapidly for China's herbal pharmaceutical industry, medicine working ability is improved constantly, and product category is increasingly numerous
Many, the discharge rate of the dregs of a decoction increases year by year.According to statistics, herbal pharmaceutical enterprise is annual will produce tens million of tons of Chinese medicine dreg, and
More than 90% manufacturer is using the dregs of a decoction as waste material rubbish, and by taking the ginseng dregs of a decoction as an example, our province just has 1000 tons or so every year,
Only just there are nearly 300 tons of ginseng dregs of a decoction in Nanjing University of Traditional Chinese Medicine's Southern Star pharmaceutical factory every year, and this causes plant to a certain extent
Surrounding enviroment can also be polluted by the huge waste of resource simultaneously because the dregs of a decoction are not handled in time, so using medicinal fungi
Amphicheirality's solid-fermented technique carries out fermentation process to Chinese medicine dreg, and is allowed to form new beneficiating ingredient and have new nature and flavor
Function, and as immunopotentiator for animals be reused by be Chinese medicine dreg most effective processing and Land use systems.This is also to fill
Divide using Chinese material medicine resource, its " turning waste into wealth " is provided good research direction and Industry Model, in will being other
Solid foundation is established in reasonable application, the exploitation of medicinal material kind.
The content of the invention
The technical problem of solution：The invention provides it is a kind of safely, effectively, economic, non-harmful plant-based animal is immunized
Reinforcing agent and its preparation method and application, while fully developing Chinese medicine ginseng resource.
Technical scheme：A kind of plant-based animal immune potentiator, is connect by cordyceps (Cordyceps militaris)
Solid medium based on kind of ginseng or the ginseng dregs of a decoction, fermented, sterilization processing and the product obtained.
The preparation method of plant-based animal immune potentiator, preparation process is：
1) fermentation system
Fungi ginseng solid fermentation system：The mass ratio of Chinese medicine ginseng is 58%, and corn flour mass ratio is 28%, wheat bran
10%, urea quality ratio is 4%；It is 1 that water volume ratio is expected in fermentation system:2, it is 5.0 with 0.1M salt acid for adjusting pH, is inoculated with pupa
Cordyceps Militaris (Cordyceps militaris) is as fermented bacterium, and it is >=9.2 × 10 to make its content6Individual/g, the middle temperature of fermentation process
Degree is maintained 25 DEG C~30 DEG C, and fermentation time is 264 hours；
Or fungi ginseng dregs of a decoction solid fermentation system：The mass ratio of the ginseng dregs of a decoction is 50%, and corn flour mass ratio is
31%, wheat bran 15%, urea quality ratio is 4%；It is 1 that water volume ratio is expected in fermentation system:2, be with 0.1M salt acid for adjusting pH
5.0, inoculation cordyceps (Cordyceps militaris) are as fermented bacterium, and it is >=15.7 × 10 to make its content6Individual/g,
Temperature is maintained 25 DEG C~30 DEG C in fermentation process, and fermentation time is 264 hours；
2) sterilization processing：
After fermentation ends, gained tunning is dried through 55 DEG C dries, and is vacuum-packed and is sealed with PE plastic packaging bags,
Load corrugated case sealing；Used under original packing60Co gamma-rays carries out radiation sterilizing processing, and radiation sterilizing absorbed dose of radiation scope is
5-10kGy, Dosimetry uniformity ＜ 1.38, that is, obtain described plant-based animal immune potentiator product.
Cordycepin content is not less than 3.2mg/g and cordycepic acid content to be not less than 52mg/g be fermentation Success criteria.
Application of the product in the immunopotentiator for preparing chicken, pig or ox.
Beneficial effect：Plant-based animal immunopotentiator of ginseng exploitation provided by the present invention and preparation method thereof is with showing
There is technology to compare, have the following advantages that and good effect：
(1) immunoenhancement result is obvious.Experiment is proved, after two kinds of tunnings are fed through spice, can make suppressed gulp down
Phagocyte and lymphocyte function recover normal, and strengthen cellular immunity function, promote the breeding of humoral immunity cell, it is ripe and
Antibody is produced, and greatly improves the immune effect of vaccine.
(2) non-environmental-pollution in producing, noresidue, while application effect is substantially, it is a kind of nuisanceless plant to person poultry safety
Material resource animal immunity potentiator.
(3) simultaneously, it is Promethean on the basis of existing medicinal fungi fermented tcm material technology to use ginseng dregs of a decoction conduct
Medicinal substrate is fermented, and one side ginseng dregs of a decoction materials are wider, and cost is low, and reaches the purpose for turning that waste be changed into values, and key is
Extract and be still rich in a large amount of panaxosides, plant flavone, choline, leukotrienes and several amino acids after panaxan in the ginseng dregs of a decoction
And trace element, these beneficiating ingredients, which are such as made an addition in the daily feed of animal, will be more suitable for Animal nutrition requirement, and immunity energy
Power is improved.
(4) content of various microorganisms in product can be significantly reduced using the radiation method for sterilizing in the present invention, bacterium is total
Number is reduced to below 1000cfu/g, and coliform is reduced to below 30MPN/100g, and mould and salmonella do not detect, and energy
Enough keep main nutrient composition and appearance luster and mouthfeel in product constant.This method is easy to operate, fast, and cost is low, can
Extensive use, is stored at normal temperatures, and the shelf-life was up to more than 1 year.
Embodiment
With reference to specific embodiment, the present invention is expanded on further.It should be understood that these embodiments are merely to illustrate the present invention
Rather than limitation the scope of the present invention.The experimental method of unreceipted actual conditions in the following example, generally according to conventional strip
Part, or according to the condition proposed by manufacturer.
With cordyceps (Cordyceps militaris), (this strain is bought in China General Microbiological culture presevation pipe
Reason center, strain number 5.699) it is fermented bacterium, by the use of ginseng or the ginseng dregs of a decoction as culture medium, both collectively form fermentation
The preparation for the plant-based animal immune potentiator product (herb fermenting product) for combining and being fermented developed：
Fermentation system 1：The matter of the preparation, wherein ginseng of solid fermentation substrate is carried out using ginseng, corn flour, wheat bran, urea
It is 58% to measure fraction, and corn flour mass fraction is 28%, and wheat bran mass fraction is 10%, urea 4%, and fermentation condition is：Fermentation
Material-water ratio is 1 in system：2, it is 5.0 with 0.1M hydrochloric acid regulation PH, inoculation cordyceps (Cordyceps militaris)
(this strain is bought in China General Microbiological culture presevation administrative center, strain number 5.699) contains it as fermented bacterium
Measure as >=9.2 × 106Temperature is maintained 25 DEG C -30 DEG C in individual/g, fermentation process, and fermentation time is 264 hours.After fermentation ends
Products therefrom carries out radiation sterilizing processing with 60Co gamma-rays through 55 DEG C of drying, and radiation sterilizing absorbed dose of radiation scope is 5-
10kGy, Dosimetry uniformity ＜ 1.38.
The examination and test of products and quality standard：Fermented sample is ground, precision weighs 0.3g into conical flask with cover, accurately
Pure methanol 30mL is added, and carries out ultrasonic extraction 30min.Then 4000r/min centrifuges 10min, takes supernatant 5mL, is evaporated molten
Agent, and 5mL is settled to flow dissolving, high speed centrifugation (13000r/min) is standby.
With the cordycepin in high performance liquid chromatography control cordycepin, the standard curve determination tunning of cordycepic acid, worm
Oxalic acid content, with cordycepin content 3.2mg/g, cordycepic acid content more than 52mg/g for fermentation Success criteria, while with Portugal
Glycan is that auxiliary material is adjusted to tunning cordycepin, cordycepic acid content, makes the cordycepin content in every batch of fermented product be
2.8mg/g, cordycepic acid content are 46mg/g.
Fermentation system 2：The preparation of solid fermentation substrate, wherein ginseng are carried out using the ginseng dregs of a decoction, corn flour, wheat bran, urea
The mass fraction of the dregs of a decoction is 50%, and corn flour mass ratio is 31%, wheat bran 15%, and urea quality ratio is 4%；Fermentation：Fermentation body
It is 1 that water volume ratio is expected in system：2, it is 5.0 with 0.1M hydrochloric acid regulation PH, inoculation cordyceps (Cordyceps militaris)
(this strain is bought in China General Microbiological culture presevation administrative center, strain number 5.699) contains it as fermented bacterium
Measure as >=15.7 × 106Temperature is maintained 25 DEG C -30 DEG C in individual/g, fermentation process, and fermentation time is 264 hours；
Two kinds of tunnings of gained carry out radiation sterilizing processing, spoke through 55 DEG C of drying with 60Co gamma-rays after fermentation ends
It is 5-10kGy, Dosimetry uniformity ＜ 1.38 according to sterilization absorbed dose of radiation scope.
The examination and test of products and quality standard：Fermented sample is ground, precision weighs 0.3g into conical flask with cover, accurately
Pure methanol 30mL is added, and carries out ultrasonic extraction 30min.Then 4000r/min centrifuges 10min, takes supernatant 5mL, is evaporated molten
Agent, and 5mL is settled to flow dissolving, high speed centrifugation (13000r/min) is standby.
With the cordycepin in high performance liquid chromatography control cordycepin, the standard curve determination tunning of cordycepic acid, worm
Oxalic acid content, with cordycepin content 3.2mg/g, cordycepic acid content more than 52mg/g for fermentation Success criteria, while with Portugal
Glycan is that auxiliary material is adjusted to tunning cordycepin, cordycepic acid content, makes the cordycepin content in every batch of fermented product be
2.8mg/g, cordycepic acid content are 46mg/g.
Embodiment 1：Fermented product zoopery obtained by Cordyceps militaris fermented ginseng (fermentation system 1)
1. 40 14 ages in days health Luo Man chick are randomly divided into control group and test group, raise according to a conventional method,
The maternal antibody against newcastle disease of each group chicken is determined before experiment, it is 2 to choose maternal antibody4Following chick is carried out as experimental chicken
Weigh, and carry out ND-Lasota vaccines (Nanjing Tianbang Bio-industry Co., Ltd.) and be inoculated with a 1 plumage part/inoculum concentration, given simultaneously
Test group chick adds above-mentioned fermented product in normal daily ration, and the mass fraction for making fermented product is 4%, persistently adds 30
My god, 10,20,30 and 40d distinguishes wing venous blood sampling after experiment, and carrying out ND-HI antibody tests (is used to determine fermented product to chicken
Body humoral immunity enhancing effect), ERFC experiments (be used for determine fermented product to chicken body cell immunoenhancement result), peripheral blood T
Lymphocyte ANAE formation rates determine and (are used to determine fermented product to chicken body cell immunoenhancement result), in off-test,
Every group takes after 10 chickens weigh at random, and water supply fasting 24h, jugular vein bloodletting is lethal, and the bursa of farbricius and spleen are gathered respectively, enters luggage
Body ratio, spleen body frequently compared with.
2. (14 age in days) each group chicken ND-HI antibody titer measurement result group differences before newcastle disease HI antibody detection tests
Not notable (P>0.05) 10,20,30 and 40d test group ewcastle disease HI antibody titers pole is significantly higher than control group (P after, being immunized<
0.01), it was demonstrated that this immunopotentiator can significantly increase humoral immune function, ND-HI antibody titers are improved, 1 is the results are shown in Table.
Influence (log2) of the immunopotentiator of table 1 to newcastle disease HI antibody variations
Note：Indicating * * with column data shoulder represents difference extremely significantly (P ＜ 0.01).
3. (14 age in days) each group newcastle disease ERFC formation rate differences are not notable before newcastle disease ERFC formation experiment experiments
(P>0.05) 10,20,30 and 40d test group ewcastle disease ERFC formation rates pole is significantly higher than control group (P after, being immunized<0.01), demonstrate,prove
Bright immunopotentiator can significantly increase cellular immune function, the results are shown in Table 2.
Influence (%) of the immunopotentiator of table 2 to chicken ERFC formation rates
Note：Indicating * * with column data shoulder represents difference extremely significantly (P ＜ 0.01).
4. (14 age in days) each group newcastle disease ANAE positive rate differences do not show before newcastle disease ANAE positive rate determination tests
Write (P>0.05), 20 after experiment, 30,40d test group ANAE positive rates pole is significantly higher than control group (P<0.01), show that this is immunized
Reinforcing agent can effectively facilitate the induction of T lymphocytes, and synergetic immunity stimulation can improve periphery blood T lymphocyte percentage,
It the results are shown in Table 3.
Influence (%) of the immunopotentiator of table 3 to chicken ANAE positive rates
Note：Indicating * * with column data shoulder represents difference extremely significantly (P ＜ 0.01).
5. the relative immunity reinforcing agent of immune organ relative weight has obvious growth promoting function, difference pole is examined through t
Significantly (P<0.01)；The utricule ratio and spleen body ratio of body are improved, but the not notable (P of difference>0.05), it the results are shown in Table
4。
Influence of the immunopotentiator of table 4 to Immune Organs of Chicken relative weight
Note：Indicating * * with column data shoulder represents difference extremely significantly (P ＜ 0.01).
Embodiment 2：Fermented product zoopery obtained by Cordyceps militaris fermented ginseng (fermentation system 1)
1 selection age in days is (28 ± 3) d length × big binary weanling pig 80 (male and female half and half), and test pig is regular grade
Experimental animal.
1) 2 treatment groups are randomly divided into, each treatment group sets 5 repetitions, are 1 repetition group per column, per 8, column son
Pig.2 treatment groups are respectively：Control group:Feed basal diet；Treatment group:Basal diet+200mg/kg fermented products.Experiment
In Jiangning, Mo Ling kind pig breedings Co., Ltd is carried out, totally enclosed type pig house, roof ventilating fan ventilation, crack metal floor, on the net
Flat to support, house temperature is maintained at 25 DEG C -30 DEG C.Powder feeding, a small amount of multiple, free choice feeding drinking-water, conventinal breeding and immune, experimental period
For 30 days.
2) respectively when experiment proceeds to the 14th and 28 day, each treatment group is taken a blood sample 5 before morning feeding, and sterile ante-chamber is quiet
Arteries and veins blood sampling is injected separately into vacuum EDTA anticoagulant tubes and anticoagulation and serum is made in vacuum biochemical tube.Peripheral blood is determined using mtt assay
Lymphocyte transformation rate；Determining T lymphocyte subsets distribution situation using Flow cytometry (is used to determine fermentation production
Product are to porcine somatic cell immunoenhancement result).All experiment piglets are in dosage swine fever cell vaccine immunity inoculations such as 25 ages in days progress.
The 7th after immunity inoculation, each treatment group blood sampling 5 in 14,21,28 days, taken a blood sample with vacuum biochemical tube vena cava anterior, separate serum
- 80 DEG C of packing is frozen, and hog cholera antibody is detected with the swine fever ELISA antibody kits of IDEXX companies of the U.S..
3) all test datas carry out ANO-VA statistical dispositions using SPSS100 softwares.The significance of difference is carried out
DuncanShi compares.Data are represented with mean+SD (Mean ± SD).P<0.05 is defined as significant difference.
Influence of 2 tunnings to weanling pig PBLC lymphocyte transformation rate
As shown in Table 5, experiment the 14th, 28 days, the PBLC conversion ratio for adding tunning group is extremely notable
Higher than control group (P<0.01).
Influence of the tunning of table 5 to the PBLC conversion ratio of weanling pig
Note：Indicating * * with column data shoulder represents difference extremely significantly (P ＜ 0.01).
Influence of 3 tunnings to weanling pig lymphocyte subpopulation quantity
As shown in Table 6, addition tunning is after 14 days, piglet periphery blood T lymphocyte (CD3+)、CD4+T cell, CD8+T
Cell, natural killer cell (CD3-CD4-CD8+), Th cells (CD3+CD4+CD8-) and Tc cell quantities (CD3+CD4-CD8+)
It is all remarkably higher than control group (P<0.05), show that tunning can be remarkably reinforced porcine somatic cell immune effect.
Influence of the tunning of table 6 to weanling pig lymphocyte subpopulation quantity
Note：Indicating * * with column data shoulder represents difference extremely significantly (P ＜ 0.01).
Influence of 4 tunnings to hog cholera antibody in weanling pig serum
As shown in Table 7, ascendant trend is presented after hog cholera vaccine is immune 21 days.Tunning is remarkably improved hog cholera vaccine and exempted from
Antibody level (the P of 21 days, 28 days after epidemic disease<0.05), show that tunning can be remarkably reinforced pig body Humoral.
Influence (blocking rate) of the tunning of table 7 to hog cholera antibody in weanling pig blood
Note：Indicating * * with column data shoulder represents difference extremely significantly (P ＜ 0.01).
Embodiment 3：Fermented product zoopery obtained by the Cordyceps militaris fermented ginseng dregs of a decoction (fermentation system 2)
The selection and packet of 1 experiment milk cow：
The healthy ox of 40 lactation periods is selected from 100 holstein cows of same cowshed, according to giving milk age, day
The close principle of amount, lactation number of days, parity carries out packet pairing, is then randomly divided into two groups every group 20.One group is test group,
One group is control group.
2 test feeds and feeding method
By Shanghai kind cattle farm without daily ration, control based on any additive, the whole-plant corn feed of natural ensiling
Group:Feed basal diet；Treatment group:Basal diet+600mg/kg/d fermented products.Experiment is carried out in Shanghai kind cattle farm, is fed
The method of supporting is identical when peaceful, free choice feeding drinking-water, conventinal breeding and immune, and experimental period is 30 days.
3 test indexes
During experiment, determine weekly by head daily yielding l times, and take milk sample, in Shanghai pasture lab analysis breast into
Part, instrument is milk composition analyzer (Zhejiang University's Food Science is produced with Fermentation Engineering research institute, MILKYWAY-l types).
4 result of the tests
Influence of 4.1 tunnings to the lactating cow output of milk
The output of milk level of each group cow in milk is close before experiment, and during testing, each group average milk production has risen.
Average daily milk production is 44.7 and 45.2kg before test group and control group examination, and average out to 48.9 and 45.7 during testing, respectively
Rise 9.4% and 1.3%.
Influence of the tunning of table 8 to the lactating cow output of milk
Note：Indicating * * with column data shoulder represents difference extremely significantly (P ＜ 0.01).
Influence of 4.2 tunnings to lactating cow butterfat percnetage
As shown in table 9, the butterfat percnetage average value of experimental period control group has before relatively testing for influence to lactating cow butterfat percnetage
Declined, and test group then rises, and difference extremely significantly (P<0.01), illustrate that tunning can slow down the breast for the later stage milk cow that gives milk
Fat rate declines.
The tunning of table 9 is given milk the influence of butterfat percnetage to lactating cow
Note：Indicating * * with column data shoulder represents difference extremely significantly (P ＜ 0.01).
Influence of 4.3 tunnings to lactating cow protein ratio
Decline of the newborn egg of late lactation from rate can effectively be slowed down using tunning as can be seen from Table 10, with lactation
Newborn egg in the change of phase, milk has declined before relatively being tested from rate, but the amplitude of test group reduction is smaller, control group reduction
Amplitude is larger, difference extremely significantly (P<0.01).Illustrate that tunning can effectively slow down the downward trend of late lactation protein ratio.
The tunning of table 10 is given milk the influence of albumen rate to lactating cow
Note：Indicating * * with column data shoulder represents difference extremely significantly (P ＜ 0.01).
Influence of 4.4 tunnings to lactating cow somatic number
The average somatic number of control group reaches 85.2 ten thousand/mL and the somatic number of test group milk cow has been obtained well
Control, average somatic number is 32.7 ten thousand/mL, drops to 500,000/below mL, has reached that one of present milk purchase is general
Standard, and difference extremely significantly (P<0.01), the reduction of milk cow somatic number, effectively reduces the loss of the output of milk.Illustrate hair
Ferment product has the immunity for significantly improving milk cow.
Influence of the tunning of table 11 to lactating cow somatic number
Note：Indicating * * with column data shoulder represents difference extremely significantly (P ＜ 0.01).
Claims (4)
1. a kind of plant-based animal immune potentiator, it is characterised in that by cordyceps（Cordyceps militaris）Connect
Solid medium based on kind of ginseng or the ginseng dregs of a decoction, fermented sterilization processing and the product obtained.
2. the preparation method of plant-based animal immune potentiator according to claim 1, it is characterised in that preparation process is：
1）Fermentation system
Fungi ginseng solid fermentation system：The mass ratio of Chinese medicine ginseng is 58%, and corn flour mass ratio is 28%, wheat bran 10%, urea
Mass ratio is 4%；It is 1 that water volume ratio is expected in fermentation system:2, it is 5.0 with 0.1M salt acid for adjusting pH, is inoculated with cordyceps
（Cordyceps militaris）As fermented bacterium, it is >=9.2 × 10 to make its content6Temperature is maintained in individual/g, fermentation process
For 25 DEG C ~ 30 DEG C, fermentation time is 264 hours；
Or fungi ginseng dregs of a decoction solid fermentation system：The mass ratio of the ginseng dregs of a decoction is 50%, and corn flour mass ratio is 31%, wheat bran
15%, urea quality ratio is 4%；It is 1 that water volume ratio is expected in fermentation system:2, it is 5.0 with 0.1M salt acid for adjusting pH, is inoculated with pupa worm
Careless bacterium（Cordyceps militaris）As fermented bacterium, it is >=15.7 × 10 to make its content6Individual/g, the middle temperature of fermentation process
Degree is maintained 25 DEG C ~ 30 DEG C, and fermentation time is 264 hours；
2）Sterilization processing：
After fermentation ends, gained tunning is dried through 55 DEG C dries, and is vacuum-packed and is sealed with PE plastic packaging bags, loads
Corrugated case is sealed；Used under original packing60Co gamma-rays carries out radiation sterilizing processing, and radiation sterilizing absorbed dose of radiation scope is 5-10
KGy, Dosimetry uniformity ＜ 1.38, that is, obtain described plant-based animal immune potentiator product.
3. the preparation method of plant-based animal immune potentiator according to claim 2, it is characterised in that cordycepin contains
Amount is not less than 3.2mg/g and cordycepic acid content to be not less than 52mg/g be fermentation Success criteria.
4. application of the product described in claim 1 in the immunopotentiator for preparing chicken, pig or ox.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710216799.1A CN106962605A (en) | 2017-04-05 | 2017-04-05 | A kind of plant-based animal immune potentiator and its preparation method and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710216799.1A CN106962605A (en) | 2017-04-05 | 2017-04-05 | A kind of plant-based animal immune potentiator and its preparation method and application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106962605A true CN106962605A (en) | 2017-07-21 |
Family
ID=59336778
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710216799.1A Pending CN106962605A (en) | 2017-04-05 | 2017-04-05 | A kind of plant-based animal immune potentiator and its preparation method and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106962605A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023111330A1 (en) * | 2021-12-17 | 2023-06-22 | Danstar Ferment Ag | Beneficial acids for enhancing biocontrol efficacy of fungal spores |
-
2017
- 2017-04-05 CN CN201710216799.1A patent/CN106962605A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023111330A1 (en) * | 2021-12-17 | 2023-06-22 | Danstar Ferment Ag | Beneficial acids for enhancing biocontrol efficacy of fungal spores |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101731461B (en) | Plant-based animal immune potentiator prepared by fermenting and developing radix astragali decoction dregs | |
| Wei et al. | Effects of Taishan Pinus massoniana pollen polysaccharide on immune response of rabbit haemorrhagic disease tissue inactivated vaccine and on production performance of Rex rabbits | |
| CN109251951B (en) | Method for efficiently producing ganoderma lucidum polysaccharide through semi-continuous liquid culture | |
| CN104623122B (en) | A kind of biological agent and preparation method thereof with anti-fatigue effect | |
| CN101940611A (en) | Plant source animal use immunopotentiator developed by cordyceps militaris fermented traditional Chinese medicine astragalus | |
| CN104161181B (en) | The feed additive of a kind of anti-pig bacterial disease, preparation method and application | |
| CN106072491B (en) | A kind of fructus arctii base Ganoderma lucidum mycelium powder and preparation method thereof | |
| CN107488598B (en) | Burdock-based cordyceps militaris mycelium and preparation method thereof | |
| CN105146509B (en) | A kind of method that probiotics stem of noble dendrobium ferment is produced using stem of noble dendrobium leaf | |
| CN108029885A (en) | Pet care product and preparation method thereof | |
| CN107624513A (en) | It is a kind of rich in the edible and medical fungi cultural hypha method of polysaccharide and application | |
| CN107012101A (en) | A kind of method for removing the peel burdock liquid fermentation medium and the new mushroom of fermented tea | |
| CN102669427A (en) | Paecilomyces militaris Liang sp.nov. fermented feed additive as well as preparation method and application thereof | |
| CN106509142A (en) | Mycelium fluid health-care product and preparation method thereof | |
| CN106993808B (en) | A kind of Organic Lemon ferment health-care nutrient liquor and preparation method thereof | |
| CN106072632A (en) | A kind of Ganoderma extract and its preparation method and application | |
| CN104161179B (en) | The feed additive of a kind of anti-canine viral disease, preparation method and application | |
| CN106962605A (en) | A kind of plant-based animal immune potentiator and its preparation method and application | |
| CN105076707B (en) | A kind of ox fattening feed based on jujube | |
| CN107550946A (en) | A kind of preparation and application of poultry immunity reinforcing agent | |
| CN105147719B (en) | A kind of preparation of Yupingfeng dregs of a decoction polysaccharide symphysis unit and its application in immunological regulation | |
| CN103229666A (en) | Cordyceps militaris peanut and preparing method thereof | |
| CN106490307A (en) | A kind of method of antimicrobial composition fermentation Entermorpha | |
| CN103734497A (en) | Cow heat-stress resistant feed additive | |
| CN104161183B (en) | The feed additive of a kind of anti-chicken viral diseases, preparation method and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170721 |
|
| WD01 | Invention patent application deemed withdrawn after publication |