CN106962467A - A kind of method that utilization ethephon (CEPHA),2-(chloroethyl) phosphonic acid control normal temperature stores and transports mango anthracnose - Google Patents
A kind of method that utilization ethephon (CEPHA),2-(chloroethyl) phosphonic acid control normal temperature stores and transports mango anthracnose Download PDFInfo
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- CN106962467A CN106962467A CN201710281261.9A CN201710281261A CN106962467A CN 106962467 A CN106962467 A CN 106962467A CN 201710281261 A CN201710281261 A CN 201710281261A CN 106962467 A CN106962467 A CN 106962467A
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- mango
- ethephon
- cepha
- chloroethyl
- phosphonic acid
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B7/00—Preservation or chemical ripening of fruit or vegetables
- A23B7/14—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
- A23B7/153—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of liquids or solids
- A23B7/154—Organic compounds; Microorganisms; Enzymes
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
- A01N57/18—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds
- A01N57/20—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds containing acyclic or cycloaliphatic radicals
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/85—Food storage or conservation, e.g. cooling or drying
Abstract
The present invention discloses a kind of method that utilization ethephon (CEPHA),2-(chloroethyl) phosphonic acid control normal temperature stores and transports mango anthracnose.This method comprises the following steps：Mango Fruit after being harvested with the immersion of 4~40mg/L ethephon solutions no more than 48 hours 3~7 minutes, dries mango after taking-up at room temperature, is packed, is stored in 20~25 DEG C for 0.02~0.04 millimeter of sealed polyethylene plastic bag with thickness.The low-concentration ethane that using the method for the present invention ethephon (CEPHA),2-(chloroethyl) phosphonic acid can be made to decompose generation first induces mango to produce disease resistance before the mango that accelerates the ripening, and effectively resists the generation of anthracnose.The simple to operate of the present invention, cost are low, and application efficiency is high, easily promote；Application of the alternative chemical bactericide in mango anti-corrosion, improves quality and safety of food；Mango fresh-keeping has saved the energy without freezer and low-temperature transport, is conducive to the protection of environment, is a kind of low-carbon preservation technology.Good economic benefit and social benefit can be produced using the method processing mango of the present invention.
Description
Technical field
It is more particularly to a kind of to utilize ethephon (CEPHA),2-(chloroethyl) phosphonic acid control normal temperature storing mango anthrax the invention belongs to mango fresh-keeping technical field
The method of disease.
Background technology
Mango (Mangifera indica L.) has soft color of the leather and yellowish pink bright-coloured attractive in appearance, meat, raciness and fragrance
Dense the features such as, the welcome of domestic and international consumer is enjoyed, be described as " king of tropical fruit (tree) ".Mango be the big tropical fruit (tree) in the world five it
One, it is distributed in almost all of tropical, subtropical countries and area (Tian Mixia etc., 2007).China is mango major producing country
One of, yield and area occupy the 4th, the world.Mango production is distributed mainly on Hainan, Guangdong, Guangxi, Sichuan, Yunnan, good fortune
Build, the provinces and regions such as Taiwan.Mango commodity value is higher, remarkable in economical benefits, is the mainstay that China's hot-zone increasing peasant income are got rich
Industry.In recent years, the provinces and regions such as Guangxi, Sichuan and Yunnan utilize abundant position and climatic superiority, greatly develop mango industry, plant
Area and yield just expand rapidly.But because mango is climacteric type fruit, the characteristics of itself has not storage tolerance, in addition awns
Fruit is how ripe in summer, weather high temperature and humidity, easily falls ill and rots after adopting, and often leads to lose after seriously adopting.Anthracnose is to lead
The Major Diseases for causing mango to be lost after adopting, susceptible fruit surface blackening, the rear corruption that softens makes fruit lose edibility and commodity valency
It is worth (Xiao Min etc., 2004).It is applied chemistry bactericide (Huang Sheng, 1999) that control mango, which adopts rear anthracnose most efficient method,
But the problems such as chemical bactericide environmental pollution, residues of pesticides and the pathogen resistance to the action of a drug brought strengthen is used for a long time
Cause the generally worry of consumer, it is using having become increasingly limited (Liu Xinhua etc., 2009).Low temperature storing and transporting is also extension
The effective ways of preserving fruit and vegetable utilizing phase, but in China Guangxi, Yunnan, Hainan and Sichuan, mango main producing region is in outlying economy
Low developed area, the mango of current low temperature storing and transporting is preserved, and lead to less than 5% after most mango harvestings under normal temperature condition
Cross normal temperature transport and be sent to each metropolitan market sale in the north such as Beijing, Shanghai, Harbin.Main cause is Cold Chain Logistics system
Cost is high, and operation and maintenance cost is high, causes China's mango Cold Chain Logistics Technique Popularizing difficult.Therefore, it is necessary to research and develop both
Safety of Food Quality, and economical and practical, efficiently normal temperature aseptic technic, loss after reduction mango is adopted easily to operate can be ensured, is promoted
Enter mango industry development and increasing peasant income.Meanwhile, such technology is either sold to the northern transportation and sales of promotion mango, or to improving me
The competitiveness of state's mango in the international market, all has great importance.
Ethene is a kind of hormone related to fruit maturation aging, adopt the processing of rear ethephon (CEPHA),2-(chloroethyl) phosphonic acid normally result in fruit senescence and
Rot (Liu Hongxia etc., 2002；Brown and Lee, 1993).Therefore, for many years, fruit industry takes a variety of methods and removed
The ethene of storage environment, to extend the fresh-keeping life-span.Such as, 1- methyl cyclopropenes (1- is used after the fruit such as apple is adopted
Methylcyclopropene, 1-MCP) (a kind of ethylene receptor inhibitor), ethylene receptor is blocked, suppresses to adopt the life of rear ethene
Into the process (Li Fujun etc., 2004) of delayed fruit aging, the fruit such as banana removes packaging bag or storage environment with potassium permanganate
Ethene, delay aging, reduce postharvest decay.For the climacteric type fruits and vegetables such as banana, mango, tomato, ethephon (CEPHA),2-(chloroethyl) phosphonic acid is effective
Ripener.These fruits and vegetables turn yellow (red) by the processing of ethephon (CEPHA),2-(chloroethyl) phosphonic acid, soften, sweeten, but can be rotted quickly after the maturation of fruit.
Therefore, there is not yet the research for controlling climacteric type fruit mango storage period rot using ethephon (CEPHA),2-(chloroethyl) phosphonic acid is reported.
The content of the invention
It is normal using ethephon (CEPHA),2-(chloroethyl) phosphonic acid control it is an object of the invention to provide one kind in order to overcome the shortcoming and deficiency of prior art
The method of temperature storing mango anthracnose.
The purpose of the present invention is achieved through the following technical solutions：
A kind of method that utilization ethephon (CEPHA),2-(chloroethyl) phosphonic acid control normal temperature stores and transports mango anthracnose, comprises the following steps：
3~7 points of Mango Fruit after harvesting no more than 48 hours is soaked with 4~40mg/L ethephon (CEPHA),2-(chloroethyl) phosphonic acids (ethephon) solution
Clock, dries mango after taking-up at room temperature, is packed, preserved for 0.02~0.04 millimeter of sealed polyethylene plastic bag with thickness
In 20~25 DEG C of storage.
Described Mango Fruit be preferably freshly harvested or be no more than the Mango Fruit of 48 hours after harvesting；
Described 4~40mg/L ethephon solutions are preferred to use following methods and prepared：Ethephon (CEPHA),2-(chloroethyl) phosphonic acid is measured, is added water dilute
Release, be configured to 4~40mg/L ethephon solutions；
Described water is preferably distilled water, running water, well water or pure water.
The thickness of described sealed polyethylene plastic bag is preferably 0.03 millimeter.
The invention mechanism of the present invention：Ethene is joined as the inducer and signaling molecule of Systemic Acquired Resistance In Plants (SAR)
With including the induction to functional moleculars such as plant cell wall lignifying, rich hydroxyl glycoprotein, pathogenesis-related protein and phytoalexins
(Jiang et al, 1994；Lers et al, 1998).Although mango, by Latent Infection of Collectotrichum Corda, is adopted in orchard
Do not fall ill immediately afterwards, in ripening process, mango gradually turns yellow softening.After the complete yellow maturity of mango, resistance declines, charcoal
Subcutaneous ulcer bacterium spore germination, grows mycelium, invades pericarp, causes morbidity.The ethephon concentration of the general mango that accelerates the ripening for 500~
1000mg/L, such concentration causes Ripening of Mango Fruit During Storage fast, is fallen ill quickly after maturation.Our research discovery, the ethene of low concentration
Profit enhances the molecular marker gene PR1 genes of disease resistance, and the expression of chitinase gene enhances POD (peroxidase)
With the expression of the disease-resistant related gene such as PDF (plant defense element) gene, the expression of ERS (Ethylene receptor gene), table are also enhanced
Bright ethephon (CEPHA),2-(chloroethyl) phosphonic acid processing have activated the defense system of mango.When the concentration of ethephon solution is 4~40mg/L, the table of these genes
Up to highest, disease resistance is most strong, can effectively control the generation of mango anthracnose.
The present invention has the following advantages and effect relative to prior art：
(1) using the present invention method, by 4~40mg/L ethephon solutions soak freshly harvested or harvesting after be no more than 48
The Mango Fruit surface of hour, is packed with 0.02~0.04 millimeter of sealed polyethylene plastic bag after drying, can make ethephon (CEPHA),2-(chloroethyl) phosphonic acid point
The low-concentration ethane that solution is produced first induces mango to produce disease resistance before the mango that accelerates the ripening, and effectively resists the generation of anthracnose.This hair
Bright simple to operate, cost is low, and application efficiency is high, easily promotes；Application of the alternative chemical bactericide in mango anti-corrosion, is carried
High quality and safety of food；Mango fresh-keeping has saved the energy without freezer and low-temperature transport, is conducive to the protection of environment, is one
Plant low-carbon preservation technology.
(2) good economic benefit and social benefit can be produced using the technical finesse mango of the present invention.Using chemistry
Bactericide handles mango, although disease, reduction can be controlled to be lost after adopting, but it is residual to produce agriculture.The technology of the present invention is to utilize institute
There is mango to accelerate the ripening the fresh-keeping mango of the ethephon (CEPHA),2-(chloroethyl) phosphonic acid that will be applied, concentration is 1/5~1/25 of the ethephon concentration for accelerating the ripening.
Both cost has been saved, rotten loss is reduced again.About 1,000,000 tons of the annual Mango Output of China, every year caused by various factors
About 300,000 tons of loss after adopting, loss about 20% after wherein being adopted caused by anthracnose；In terms of 8 yuan of per kilogram mango, using this
The technology of invention, can control loss 90% caused by mango anthracnose, and technology of the invention can be reduced and lost after mango is adopted
180000 tons, equivalent to 14.4 hundred million yuan of direct economic benefit can be produced every year.
Brief description of the drawings
Fig. 1 is the effect that mango anthracnose is controlled after being handled with low concentration (4mg/L) ethephon (CEPHA),2-(chloroethyl) phosphonic acid (Eth)；Wherein, compare and be
Water process is distilled, storage time unit (d) is day, and such as 9d is represented 9 days.
Fig. 2 is the influence to mango anthracnose with the ethephon (CEPHA),2-(chloroethyl) phosphonic acid (Eth) of various concentrations, wherein, after data is processing in figure
Gather within 14 days.
Embodiment
With reference to embodiment and accompanying drawing, the present invention is described in further detail, but embodiments of the present invention are not limited
In this.
Embodiment 1
With the mango of 4mg/L ethephon (CEPHA),2-(chloroethyl) phosphonic acid aqueous solution soaking freshly harvesteds (Mangifera indica L., similarly hereinafter.) 3 points of fruit
Clock, taking-up is packed with 0.03 millimeter of sealed polyethylene plastic bag after drying and is stored in 20 DEG C of storage；
Mango lesion diameter through 4mg/L ethephon (CEPHA),2-(chloroethyl) phosphonic acid aqueous solution soakings is than control 52% (being shown in Table 1) of reduction.
Storage time is the effect of 5d, 7d, 9d, 11d control mango anthracnose, as shown in Figure 1.Can from Fig. 1
Go out, with the mango of low-concentration ethane profit processing, in whole storage period vaccination scab significantly less than control.Show, ethene
Effectively control mango adopts rear anthracnose.
Storage time is the effect of 14d control mango anthracnose, as shown in Figure 2.
Embodiment 2
The Mango Fruit of 24 hours 5 minutes after 40mg/L ethephon (CEPHA),2-(chloroethyl) phosphonic acids aqueous solution soaking is harvested, take out after drying with 0.03
Millimeter sealed polyethylene plastic bag packaging is stored in 25 DEG C of storage；
The lesion diameter of mango through 40mg/L ethephon (CEPHA),2-(chloroethyl) phosphonic acid aqueous solution soakings is than control 46% (being shown in Table 1) of reduction.
Storage time is the effect of 14d control mango anthracnose, as shown in Figure 2.
Embodiment 3
The Mango Fruit of 48 hours 7 minutes after 20mg/L ethephon (CEPHA),2-(chloroethyl) phosphonic acids aqueous solution soaking is harvested, take out after drying with 0.03
Millimeter sealed polyethylene plastic bag packaging is stored in 22 DEG C of storage；
The lesion diameter of mango through 20mg/L ethephon (CEPHA),2-(chloroethyl) phosphonic acid aqueous solution soakings is than control 49% (being shown in Table 1) of reduction.
Effect example
The situation of difference control anthracnose of the testing example 1~3 through 4~40mg/L ethephon (CEPHA),2-(chloroethyl) phosphonic acid aqueous solution soaking mango,
1 is the results are shown in Table, as it can be seen from table 1 the mango lesion diameter of 4mg/L ethephon (CEPHA),2-(chloroethyl) phosphonic acid aqueous solution soakings is than control reduction by 52%；
The lesion diameter of the mango of 40mg/L ethephon (CEPHA),2-(chloroethyl) phosphonic acid aqueous solution soakings is than control reduction by 46%；The awns of 20mg/L alkene profit aqueous solution soaking
The lesion diameter of fruit is than control reduction by 49%.
The embodiment 1~3 of table 1 controls the result of mango anthracnose
| Processing | Anthracnose lesion diameter * |
| Control | 2.75A** |
| Embodiment 1 (ethephon (CEPHA),2-(chloroethyl) phosphonic acid 4mg/L) | 1.32B |
| Embodiment 2 (ethephon (CEPHA),2-(chloroethyl) phosphonic acid 40mg/L) | 1.48B |
| Embodiment 3 (ethephon (CEPHA),2-(chloroethyl) phosphonic acid 20mg/L) | 1.4B |
Note：* mango measures lesion diameter after being handled 14 days with ethephon (CEPHA),2-(chloroethyl) phosphonic acid；Different alphabetical surface differences are extremely notable after * numerals
(P≤0.01)。
Comparative example 1
The Mango Fruit of 48 hours 3 minutes after 2mg/L ethephon (CEPHA),2-(chloroethyl) phosphonic acids aqueous solution soaking is harvested, take out after drying with 0.03 milli
Rice sealed polyethylene plastic bag packaging is stored in 25 DEG C of storage；
The lesion diameter of mango through 2mg/L ethephon (CEPHA),2-(chloroethyl) phosphonic acid aqueous solution soakings is than control 20% (being shown in Table 2) of reduction.
Comparative example 2
The Mango Fruit of 24 hours 5 minutes after being harvested with 400mg/L ethephon (CEPHA),2-(chloroethyl) phosphonic acids aqueous solution soaking, take out after drying with 0.04
Millimeter sealed polyethylene plastic bag packaging is stored in 20 DEG C of storage；
The lesion diameter of mango through 400mg/L ethephon (CEPHA),2-(chloroethyl) phosphonic acid aqueous solution soakings is than control 25% (being shown in Table 2) of increase.
Storage time is the effect of 14d control mango anthracnose, as shown in Figure 2.
The comparative example 1~2 of table 2 controls the result of mango anthracnose
| Processing | Anthracnose lesion diameter * |
| Control | 2.75B** |
| Comparative example 1 (ethephon (CEPHA),2-(chloroethyl) phosphonic acid 2mg/L) | 2.2C |
| Comparative example 2 (ethephon (CEPHA),2-(chloroethyl) phosphonic acid 400mg/L) | 3.9A |
Note：* mango measures lesion diameter after being handled 14 days with ethephon (CEPHA),2-(chloroethyl) phosphonic acid；Different alphabetical surface differences are extremely notable after * numerals
(P≤0.01)。
Contrast effect embodiment
Test comparison embodiment 1~2 is sent out anthracnose through 2mg/L and 400mg/L ethephon (CEPHA),2-(chloroethyl) phosphonic acid aqueous solution soaking mango respectively
Raw influence, the results are shown in Table 2, from table 2 it can be seen that the mango lesion diameter of 2mg/L ethephon (CEPHA),2-(chloroethyl) phosphonic acid aqueous solution soakings drops than control
It is low by 20%；The lesion diameter of the mango of 400mg/L ethephon (CEPHA),2-(chloroethyl) phosphonic acid aqueous solution soakings is than control increase by 42%.If that is, second
Alkene profit concentration reaches the consumption for the mango that accelerates the ripening, and can aggravate mango anthracnose.
Mango is handled with the ethephon (CEPHA),2-(chloroethyl) phosphonic acid (Eth) (4mg/L, 40mg/L, 400mg/L) of various concentrations, to mango anthracnose
Influence is different, as a result as shown in Figure 2.From figure 2 it can be seen that compared with the control, concentration is 4mg/L and 40mg/L ethephon (CEPHA),2-(chloroethyl) phosphonic acid
Lesion diameter is significantly reduced, and after concentration is 400mg/L ethephon (CEPHA),2-(chloroethyl) phosphonic acid processing, significantly increases lesion diameter.Show, 4~
40mg/L ethephon (CEPHA),2-(chloroethyl) phosphonic acid can effectively control the generation of mango anthracnose, and the ethephon concentration 400mg/L for the mango that is used to accelerating the ripening shows
Work has aggravated anthracnose generation.
Above-described embodiment is preferably embodiment, but embodiments of the present invention are not by above-described embodiment of the invention
Limitation, other any Spirit Essences without departing from the present invention and the change made under principle, modification, replacement, combine, simplification,
Equivalent substitute mode is should be, is included within protection scope of the present invention.
Claims (4)
1. a kind of method that utilization ethephon (CEPHA),2-(chloroethyl) phosphonic acid control normal temperature stores and transports mango anthracnose, it is characterised in that comprise the following steps：
Mango Fruit after being harvested with the immersion of 4~40mg/L ethephon solutions no more than 48 hours 3~7 minutes, by awns after taking-up
Fruit is dried at room temperature, is packed, is stored in 20~25 DEG C for 0.02~0.04 millimeter of sealed polyethylene plastic bag with thickness.
2. the method that utilization ethephon (CEPHA),2-(chloroethyl) phosphonic acid control normal temperature according to claim 1 stores and transports mango anthracnose, it is characterised in that：
Described 4~40mg/L ethephon solutions are prepared using following methods：Ethephon (CEPHA),2-(chloroethyl) phosphonic acid is measured, is diluted with water, 4 are configured to
~40mg/L ethephon solutions.
3. the method that utilization ethephon (CEPHA),2-(chloroethyl) phosphonic acid control normal temperature according to claim 2 stores and transports mango anthracnose, it is characterised in that：
Described water is distilled water, running water, well water or pure water.
4. the method that utilization ethephon (CEPHA),2-(chloroethyl) phosphonic acid control normal temperature according to claim 1 stores and transports mango anthracnose, it is characterised in that：
The thickness of described sealed polyethylene plastic bag is 0.03 millimeter.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109006996A (en) * | 2018-08-28 | 2018-12-18 | 陕西师范大学 | A method of regulation Kiwi berry eats shelf life |
| CN114375988A (en) * | 2022-01-13 | 2022-04-22 | 武汉轻工大学 | Peeled lotus root preservation method |
| CN114747409A (en) * | 2022-04-28 | 2022-07-15 | 三明市农业科学研究院 | Prevention and treatment method for yam anthracnose |
| CN115736006A (en) * | 2022-11-23 | 2023-03-07 | 中国热带农业科学院环境与植物保护研究所 | Method for controlling anthracnose of mango stored at normal temperature |
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| CN104472662A (en) * | 2014-12-17 | 2015-04-01 | 广西百色国家农业科技园区管理委员会 | Preservation method for mango fruits |
| CN105166020A (en) * | 2015-08-10 | 2015-12-23 | 攀枝花市鸿鹄农业开发有限责任公司 | Fresh keeping method for long-range transportation of mangos |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109006996A (en) * | 2018-08-28 | 2018-12-18 | 陕西师范大学 | A method of regulation Kiwi berry eats shelf life |
| CN114375988A (en) * | 2022-01-13 | 2022-04-22 | 武汉轻工大学 | Peeled lotus root preservation method |
| CN114747409A (en) * | 2022-04-28 | 2022-07-15 | 三明市农业科学研究院 | Prevention and treatment method for yam anthracnose |
| CN115736006A (en) * | 2022-11-23 | 2023-03-07 | 中国热带农业科学院环境与植物保护研究所 | Method for controlling anthracnose of mango stored at normal temperature |
| CN115736006B (en) * | 2022-11-23 | 2024-03-08 | 中国热带农业科学院环境与植物保护研究所 | Method for controlling anthracnose of mango stored at normal temperature |
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