CN106957188A - A kind of method for preparing organic fertilizer by composite fermentation by substrate of straw - Google Patents
A kind of method for preparing organic fertilizer by composite fermentation by substrate of straw Download PDFInfo
- Publication number
- CN106957188A CN106957188A CN201710258783.7A CN201710258783A CN106957188A CN 106957188 A CN106957188 A CN 106957188A CN 201710258783 A CN201710258783 A CN 201710258783A CN 106957188 A CN106957188 A CN 106957188A
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- Prior art keywords
- straw
- bacillus
- substrate
- organic fertilizer
- fermentation
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Classifications
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biotechnology (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Pest Control & Pesticides (AREA)
- Fertilizers (AREA)
Abstract
A kind of side for preparing organic fertilizer by composite fermentation by substrate of straw --- take straw to crush particle, impregnate nutrition fortifier, with obtaining mixture A after the heat treatment of 100 DEG C of steam；Simultaneously with the purple streptomycete of finished product nutrient broth medium culture heat, bacillus subtilis, white-rot fungi, trichodermaharzianum strain, and cellulase, hemicellulase, lignoenzyme are added in bacterium solution, obtain zymocyte liquid B, spray in mixture A, and stir, it is placed in after the fermenting tank for fermentation of ventilation and is made；The degradation rate of cellulose in stalk is improved, is the effective means for improving straw utilization rate while improving the content of humic acid.
Description
Technical field
The present invention relates to a kind of technology of preparing of organic fertilizer, particularly one kind passes through composite fermentation by substrate of straw
The method for preparing organic fertilizer.
Background technology
Straw is often abandoned or burnt as waste, in this process as yield very abundant renewable resource
In not only waste preciousness biomass resource and also cause serious environmental pollution, as a big public hazards.It is big in agricultural production
Amount uses chemical fertilizer, causes environmental pollution, the problems such as utilization rate declines.And in agricultural crop straw containing a large amount of nitrogen, phosphorus, potassium and other
Trace element, therefore, bio-feritlizer is made using stalk, is conducive to improving soil physico-chemical property, is reduced fertilizer application amount, is modern
One of emphasis direction that green agriculture develops afterwards.Stalk carries out also field processing as fertilizer, is primarily limited to cellulose in stalk
Degraded and treatment technology.
The content of the invention
The technical problem to be solved in the present invention is in view of the shortcomings of the prior art, it is proposed that a kind of humus content is high, wide
The applicable method for preparing organic fertilizer by composite fermentation by substrate of straw of spectrum.
The technical problem to be solved in the present invention is achieved through the following technical solutions, and one kind is using straw as substrate
The method that organic fertilizer is prepared by composite fermentation, is characterized in：
(1) it is 3-5mm to smash straw to granularity, 10-30min is impregnated in 1000 times of liquid of nutrition fortifier, after pulling out
Moisture is removed in pouring, with airing after heat treatment 30-60 minutes of 100 DEG C of steam to 30 DEG C, obtains mixture A, stand-by；
(2) activated spawn is expanded with finished product nutrient broth medium and cultivated, enzyme preparation is added in cultured bacterium solution and is made
Into zymocyte liquid B, enzyme preparation accounts for the 0.5-12.5% of cultured bacterium solution weight.
The activated spawn is one kind or any several combination in streptomycete, bacillus, fungi,
The enzyme preparation is one kind or any several combination in cellulase, hemicellulase, lignoenzyme,
(3) zymocyte liquid B is sprayed in mixture A, and stirred, be placed in the fermenting tank for fermentation of ventilation, zymocyte liquid B is with mixing
Compound A mass ratio is 2-10：1000, cultivation temperature is controlled at 37 DEG C, and material water ratio 50%--60%, environment is relatively wet
＞ 80% is spent, ventilation maintains 1200m3/ h.t, fermentation time was controlled at 70-75 hours, and control humidity is steamed with ventilation shaft
Based on vapour or spray are turned, and humidity is kept in surface plastic covering film；
(4) fermentation rear venting force drying in 7 days.So that material moisture ＜ 20%, then stirs mixing, or add by separating twice
Compounding obtains product after the reinforcing of Ensure Liquid material.
The technical problem to be solved in the present invention can also come further to realize by the following technical programs, the strepto- bacterium bag
Include following strain：One or two kinds of mixing in hot purple streptomycete, streptomyces microflavus.
The technical problem to be solved in the present invention can also come further to realize by the following technical programs, the bacillus
Including following strain：The golden gemma bar of bacillus subtilis, bacillus licheniformis, bacillus laterosporus, colloid bacillus cereus, Su Yun
One or more kinds of mixing in bacterium, bacillus megaterium.
The technical problem to be solved in the present invention can also come further to realize by the following technical programs, and the fungi includes
Following strain：Trichodermaharzianum, white-rot fungi, beauveria bassiana, geotrichum candidum, candida krusei, brewer's yeast, the false silk in the torrid zone
One or more kinds of mixing in yeast, production protein yeast.
The technical problem to be solved in the present invention can also come further to realize by the following technical programs, the fortification
Agent is containing sugared MS culture without agar base or plant physiology precursor substance.
The technical problem to be solved in the present invention can also come further to realize by the following technical programs, plant physiology precursor
Amino acid includes：Tryptophan, glycine, asparatate, leucine, histidine, glutamic acid, lysine, threonine, silk ammonia
It is one or more kinds of mixed in acid, lysine, phenylalanine, alanine, methionine, a word used in person's names propylhomoserin, tyrosine, proline
Close.
The present invention compared with prior art, using beneficial to the microorganism relevant with cellulose degradation, improves fiber in stalk
The degradation rate of element, is the effective means for improving straw utilization rate while improving the content of humic acid.It is very fast to straw conversion
Product has amino acid, vitamin, the parahormone and peptide material for promoting plant growth.The advantage of the invention is that：1st, work
Skill is simply without cracking, soda acid or ammonification.2nd, production thing, which has, improves soil texture and increase water retention property, can walk quickly and keep away ground
Lower insect and vole.3rd, with the degraded of soil chemistry toxin and heavy metal detoxification effect.4th, promote nutrien utilization, reduce chemical fertilizer and use
Amount.5th, fruit quality and yield are improved, fruit nitrite and harmful heavy metal content reduction improve product foodsafety.
6th, promote soil micro-ecosystem flora to recover and balance, improve root system of plant activity.
Embodiment
Concrete technical scheme of the invention described further below, in order to which those skilled in the art is further understood that
The present invention, without constituting the limitation to its right.
A kind of method for preparing organic fertilizer by composite fermentation by substrate of straw, is characterized in：
(1) it is 3-5mm to smash straw to granularity, 10-30min is impregnated in 1000 times of liquid of nutrition fortifier, after pulling out
Moisture is removed in pouring, with airing after heat treatment 30-60 minutes of 100 DEG C of steam to 30 DEG C, obtains mixture A, stand-by；
(2) activated spawn is expanded with finished product nutrient broth medium and cultivated, enzyme preparation is added in cultured bacterium solution and is made
Into zymocyte liquid B, enzyme preparation accounts for the 0.5-12.5% of cultured bacterium solution weight.
The activated spawn is one kind or any several combination in streptomycete, bacillus, fungi,
The enzyme preparation is one kind or any several combination in cellulase, hemicellulase, lignoenzyme,
(3) zymocyte liquid B is sprayed in mixture A, and stirred, be placed in the fermenting tank for fermentation of ventilation, zymocyte liquid B is with mixing
Compound A mass ratio is 2-10：1000, cultivation temperature is controlled at 37 DEG C, and material water ratio 50%--60%, environment is relatively wet
＞ 80% is spent, ventilation maintains 1200m3/ h.t, fermentation time was controlled at 70-75 hours, and control humidity is steamed with ventilation shaft
Based on vapour or spray are turned, and humidity is kept in surface plastic covering film；
(4) fermentation rear venting force drying in 7 days.So that material moisture ＜ 20%, then stirs mixing, or add by separating twice
Compounding obtains product after the reinforcing of Ensure Liquid material.
The streptomycete includes following strain：One or two kinds of mixing in hot purple streptomycete, streptomyces microflavus.
The bacillus includes following strain：Bacillus subtilis, bacillus licheniformis, bacillus laterosporus, colloid
One or more kinds of mixing in bacillus, bacillus thuringiensis, bacillus megaterium.
The fungi includes following strain：The false silk ferment of trichodermaharzianum, white-rot fungi, beauveria bassiana, geotrichum candidum, Cruise
One or more kinds of mixing in mother, brewer's yeast, candida tropicalis, production protein yeast.
The nutrition fortifier is containing sugared MS culture without agar base or plant physiology precursor substance.
Plant physiology precusor amino acids include：Tryptophan, glycine, asparatate, leucine, histidine, glutamic acid,
One in lysine, threonine, serine, lysine, phenylalanine, alanine, methionine, a word used in person's names propylhomoserin, tyrosine, proline
Plant or two or more mixing.
Embodiment 1
Every mu of green vegetables apply this product 400kg, reduce fertilizer amount 20%, and other management pass through harvesting with reference to general planting method
Measuring and calculating：(per 100g green vegetables nutrients unit mg)
Using：
The per mu yield 2700kg of 320 vitamin C of vitamin A, 35 protein 1.8
Control：
The per mu yield 2000kg of 280 vitamin C of vitamin A, 28 protein 1.3
Embodiment 2
This product 500kg is applied on tomato for every mu,
Performance leaf emergence rate is fast, and increment is big, and flowering fruit is early and many, and single fruit is greatly and maturation is early etc..
Claims (8)
1. a kind of method for preparing organic fertilizer by composite fermentation by substrate of straw, it is characterised in that：
(1) it is 3-5mm to smash straw to granularity, impregnates 10-30min in 1000 times of liquid of nutrition fortifier, pulls out
Drench afterwards and remove moisture, with airing after heat treatment 30-60 minutes of 100 DEG C of steam to 30 DEG C, obtain mixture A, it is stand-by；
(2) activated spawn is expanded with finished product nutrient broth medium and cultivated, enzyme preparation system is added in cultured bacterium solution
Zymocyte liquid B is made, enzyme preparation accounts for the 0.5-12.5% of cultured bacterium solution weight.
2. described in activated spawn be one kind or any several combination in streptomycete, bacillus, fungi,
The enzyme preparation is one kind or any several combination in cellulase, hemicellulase, lignoenzyme,
(3) zymocyte liquid B is sprayed in mixture A, and stirred, be placed in the fermenting tank for fermentation of ventilation, zymocyte liquid B
Mass ratio with mixture A is 2-10：1000, cultivation temperature is controlled at 37 DEG C, and material water ratio 50%--60%, environment is relative
Humidity ＞ 80%, ventilation maintains 1200 m3/h.t, and fermentation time was controlled at 70-75 hours, and control humidity is with ventilation duct
Based on road steam or spray are turned, and humidity is kept in surface plastic covering film；
(4) fermentation rear venting force drying in 7 days.
3. causing material moisture ＜ 20%, then compound and obtain after separating twice stirring mixing, or addition nutriment reinforcing
Product.
4. the method according to claim 1 for preparing organic fertilizer by composite fermentation by substrate of straw, its feature
It is：The streptomycete includes following strain：One or two kinds of mixing in hot purple streptomycete, streptomyces microflavus.
5. the method according to claim 1 for preparing organic fertilizer by composite fermentation by substrate of straw, its feature
It is：The bacillus includes following strain：Bacillus subtilis, bacillus licheniformis, bacillus laterosporus, colloid gemma
One or more kinds of mixing in bacillus, bacillus thuringiensis, bacillus megaterium.
6. the method according to claim 1 for preparing organic fertilizer by composite fermentation by substrate of straw, its feature
It is：The fungi includes following strain：Trichodermaharzianum, white-rot fungi, beauveria bassiana, geotrichum candidum, candida krusei,
One or more kinds of mixing in brewer's yeast, candida tropicalis, production protein yeast.
7. the method according to claim 1 for preparing organic fertilizer by composite fermentation by substrate of straw, its feature
It is：The nutrition fortifier is containing sugared MS culture without agar base or plant physiology precursor substance.
8. the method according to claim 5 for preparing organic fertilizer by composite fermentation by substrate of straw, its feature
It is：Plant physiology precusor amino acids include：Tryptophan, glycine, asparatate, leucine, histidine, glutamic acid, rely
One kind in propylhomoserin, threonine, serine, lysine, phenylalanine, alanine, methionine, a word used in person's names propylhomoserin, tyrosine, proline
Or two or more mixing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710258783.7A CN106957188A (en) | 2017-04-19 | 2017-04-19 | A kind of method for preparing organic fertilizer by composite fermentation by substrate of straw |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710258783.7A CN106957188A (en) | 2017-04-19 | 2017-04-19 | A kind of method for preparing organic fertilizer by composite fermentation by substrate of straw |
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| CN106957188A true CN106957188A (en) | 2017-07-18 |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107963948A (en) * | 2017-12-30 | 2018-04-27 | 常州奕轩纺织品有限公司 | A kind of preparation method of stalk microbe decomposing microbial inoculum |
| CN109294928A (en) * | 2018-10-23 | 2019-02-01 | 中国科学院合肥物质科学研究院 | A kind of microbe soil activation microbial inoculum and the preparation method and application thereof |
| CN109761674A (en) * | 2017-11-09 | 2019-05-17 | 郴州市通源生物科技有限公司 | A kind of liquid fertilizer and preparation method thereof promoting tomato bud differentiation |
| CN110819559A (en) * | 2019-10-31 | 2020-02-21 | 郑州工程技术学院 | High-temperature-resistant straw decomposition agent and preparation method thereof |
| CN111066620A (en) * | 2019-12-25 | 2020-04-28 | 新疆农业科学院土壤肥料与农业节水研究所(新疆维吾尔自治区新型肥料研究中心) | Cotton straw decomposing composite matrix and application thereof |
| CN111304103A (en) * | 2018-12-12 | 2020-06-19 | 上海臻衍生物科技有限公司 | Surface active complex enzyme, bionic peat humic acid, and preparation method and application thereof |
| CN111869356A (en) * | 2020-07-24 | 2020-11-03 | 贵州中建建筑科研设计院有限公司 | Agricultural gypsum powder and method for improving barren soil by using same |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105669321A (en) * | 2016-01-07 | 2016-06-15 | 扬州国本生物科技有限公司 | Bio-organic fertilizer with plant straw as substrate and preparation method thereof |
-
2017
- 2017-04-19 CN CN201710258783.7A patent/CN106957188A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105669321A (en) * | 2016-01-07 | 2016-06-15 | 扬州国本生物科技有限公司 | Bio-organic fertilizer with plant straw as substrate and preparation method thereof |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109761674A (en) * | 2017-11-09 | 2019-05-17 | 郴州市通源生物科技有限公司 | A kind of liquid fertilizer and preparation method thereof promoting tomato bud differentiation |
| CN107963948A (en) * | 2017-12-30 | 2018-04-27 | 常州奕轩纺织品有限公司 | A kind of preparation method of stalk microbe decomposing microbial inoculum |
| CN109294928A (en) * | 2018-10-23 | 2019-02-01 | 中国科学院合肥物质科学研究院 | A kind of microbe soil activation microbial inoculum and the preparation method and application thereof |
| CN111304103A (en) * | 2018-12-12 | 2020-06-19 | 上海臻衍生物科技有限公司 | Surface active complex enzyme, bionic peat humic acid, and preparation method and application thereof |
| CN111304103B (en) * | 2018-12-12 | 2023-04-14 | 上海臻衍生物科技有限公司 | Surface active complex enzyme, bionic peat humic acid, and preparation method and application thereof |
| CN110819559A (en) * | 2019-10-31 | 2020-02-21 | 郑州工程技术学院 | High-temperature-resistant straw decomposition agent and preparation method thereof |
| CN111066620A (en) * | 2019-12-25 | 2020-04-28 | 新疆农业科学院土壤肥料与农业节水研究所(新疆维吾尔自治区新型肥料研究中心) | Cotton straw decomposing composite matrix and application thereof |
| CN111869356A (en) * | 2020-07-24 | 2020-11-03 | 贵州中建建筑科研设计院有限公司 | Agricultural gypsum powder and method for improving barren soil by using same |
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Application publication date: 20170718 |
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