CN106950371B - Pd-l1、cdk5和ctla4中的至少一种在制备肿瘤诊断试剂盒中的用途 - Google Patents
Pd-l1、cdk5和ctla4中的至少一种在制备肿瘤诊断试剂盒中的用途 Download PDFInfo
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- CN106950371B CN106950371B CN201710085362.9A CN201710085362A CN106950371B CN 106950371 B CN106950371 B CN 106950371B CN 201710085362 A CN201710085362 A CN 201710085362A CN 106950371 B CN106950371 B CN 106950371B
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
- G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
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Abstract
Description
RNA | XμL(含1ng至5μgRNA) |
DEPC水 | 10-XμL |
Total | 10μL |
25℃ | 10min |
37℃ | 120min |
85℃ | 5min |
4℃ | forever |
mix(2*) | 10μL |
正向引物 | 0.2μL |
反向引物 | 0.2μL |
cDNA | 2μL |
灭菌超纯水 | 7μL |
Total | 25μL |
Claims (6)
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CN201710085362.9A CN106950371B (zh) | 2017-02-17 | 2017-02-17 | Pd-l1、cdk5和ctla4中的至少一种在制备肿瘤诊断试剂盒中的用途 |
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CN201710085362.9A CN106950371B (zh) | 2017-02-17 | 2017-02-17 | Pd-l1、cdk5和ctla4中的至少一种在制备肿瘤诊断试剂盒中的用途 |
Publications (2)
Publication Number | Publication Date |
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CN106950371A CN106950371A (zh) | 2017-07-14 |
CN106950371B true CN106950371B (zh) | 2019-03-08 |
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CN201710085362.9A Active CN106950371B (zh) | 2017-02-17 | 2017-02-17 | Pd-l1、cdk5和ctla4中的至少一种在制备肿瘤诊断试剂盒中的用途 |
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Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
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CN109652504B (zh) * | 2018-12-27 | 2021-03-16 | 杭州迪相实业有限公司 | 一种同时检测外泌体膜蛋白和mRNA的方法 |
CN109884315A (zh) * | 2019-02-27 | 2019-06-14 | 华中科技大学 | 一种pd-l1外泌体的体外快速检测平台及检测方法 |
WO2020215313A1 (zh) * | 2019-04-26 | 2020-10-29 | 嘉兴允英医学检验有限公司 | 一种pd-l1表达水平检测的方法和试剂盒 |
CN112725418A (zh) * | 2021-01-25 | 2021-04-30 | 深圳乐土生物科技有限公司 | 基于游离rna检测pd-l1表达量的方法及其试剂盒 |
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WO2014039983A1 (en) * | 2012-09-07 | 2014-03-13 | The Trustees Of Dartmouth College | Vista modulators for diagnosis and treatment of cancer |
CA2925057A1 (en) * | 2013-10-01 | 2015-04-09 | Simon T. BARRY | Methods of treating and diagnosing alpha-v-beta-6 overexpressing cancer |
EP3240908A2 (en) * | 2014-12-30 | 2017-11-08 | F. Hoffmann-La Roche AG | Methods and compositions for prognosis and treatment of cancers |
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