CN1069198C - Oral liquid for strengthening liver and preparaing process thereof - Google Patents
Oral liquid for strengthening liver and preparaing process thereof Download PDFInfo
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- CN1069198C CN1069198C CN96119577A CN96119577A CN1069198C CN 1069198 C CN1069198 C CN 1069198C CN 96119577 A CN96119577 A CN 96119577A CN 96119577 A CN96119577 A CN 96119577A CN 1069198 C CN1069198 C CN 1069198C
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- oral liquid
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Abstract
The present invention provides oral liquid for strengthening livers and preparation a method thereof. The oral liquid for strengthening livers uses livers of newborn dairy cows as principal raw materials so as to match proteinose, citric acid and distilled water of auxiliary material. The preparation method comprises the following steps: crushing the livers of the dairy cows, carrying out homogenate, heating and separation, and matching with the auxiliary materials so as to produce finished products by aseptic filtration. The present invention contains liver regeneration stimulating factors of high activity and eighteen kinds of amino acid, and thus, the oral liquid for strengthening livers can promote the growth and the regeneration of liver cells, and have the advantages of good mouth feel, safety, no toxicity and simple and convenient technology; the present invention has no adverse influence for diabetics. The present invention uses biotechnology, such as colloidal mills, cyclic heating, high-speed freezing centrifugation, and thus, effectively guarantees that the oral liquid has higher bioactivity under the condition of lower concentration.
Description
The present invention relates to a kind of healthful oral liquid for liver and preparation method thereof, this oral liquid is to be raw material with the milk cattle liver in newborn 24 hours, utilize modern biotechnology to extract the health product that contain abundant liver regeneration-stimulating factor, suitable hepatopath, healthy hepatitis B virus carriers, alcohol user drink.
In China, hepatitis B is popular infectious disease the most widely, and China has 300,000,000 people to infect hepatitis B approximately, and about 1.2 hundred million people carry hepatitis B virus, has every year 300000 people to die from hepatopathy approximately.By national annual hepatitis number of patients statistics, acute hepatitis 1,200,000 examples expend 13.58 hundred million yuan of medical care expensess; Chronic hepatitis 1,200 ten thousand examples expend 200.87 hundred million yuan; Primary hepatocarcinoma 160,000 examples expend 8.19 hundred million yuan, annual cause about 222.64 hundred million yuan altogether of direct economic losses (see for details " China Youth News " nineteen ninety-five JIUYUE seven edition on the 9th) because of above-mentioned three kinds of hepatopathys.Hepatopathy extensively is detrimental to health for a long time, yet up to now, does not still have a kind of medicine that hepatitis virus is had definite pharmacological action in the world.Most medicines all attach toxic side effect, not only do not have the effect of therapy rehabilitation, can increase hepatocellular workload on the contrary, and this makes the matter worse beyond doubt to impaired liver.Therefore, the liver protecting, improve reparation and the regeneration capacity of hepatocyte self, increase the resistance of liver noxious substance, be protect the liver, the key of nourishing the liver.
LaBrecque in 1975 etc. have at first reported and extracted a kind of material that can stimulate liver regeneration from rat livers, and called after " liver regeneration-stimulating factor (Hepatie Stimulator Substance is called for short HSS).Nineteen eighty-two LaBrecque etc. proves further that again liver regeneration-stimulating factor can promote synthesizing of DNA.People's such as the journal report An Wei of Dalian Medical College in 1992 experiment is studied the hepatoprotective effect of ablactation SD rat HSS just from integral body, organ, cell, subcellsular level, and experimental result shows: (1) HSS can make CCL
4Causing mice serum GPT and GOT rising amplitude reduces.(2) HSS can make CCL
4Or the degree of galactosamine damage hepatic tissue alleviates, see from hepatic tissue section: these two kinds of toxic agent make the lobules of liver structural deterioration, the rope stripe-arrangement disappears, hepatocyte is the water sample change in the lobule, the subregion is the balloon degeneration, exist spotty necrosis in it, there is massive inflammatory cells infiltrated the portal area, central vein hyperemia; And after giving HSS, above-mentioned phenomenon all alleviates to some extent.(3) HSS can make CCL
4Infringement liver cell mitochondria succinic dehydrogenase activity changes to be recovered, and obtains proof with histology's method.4. insulin-glucagon share and can reduce galactosamine and cause the liver poisoning mouse death rate; reduce the amplitude that poisoning Mus serum GPT raises; alleviate the experiments such as degree of liver tissue injury; the contrast and the evidence that protect the liver as HSS with this known fact; thereby confirm that for certain HSS can promote the hepatocyte synthetic DNA; promote impaired hepatocellular reparation and regeneration, can protect CCL
4With the infringement of galactosamine, stop hepatocellular necrosis, the regeneration of cell cultured supernatant, the repair mechanism of startup hepatic injury to liver.
The purpose of this invention is to provide and a kind ofly can promote hepatic cell growth and regeneration, strengthen the function of detoxification of liver, the healthful oral liquid for liver that hepatic injury is had prevention and repair, selecting newborn 24 hours milk cattle livers for use is raw material, to reach the purpose of nourishing liver, building body, it can also prevent the formation of alcoholic liver and fatty liver simultaneously.
Another object of the present invention provides a kind of method for preparing above-mentioned healthful oral liquid for liver.
The objective of the invention is to realize by following means.A kind of healthful oral liquid for liver, it mainly is made up of milk cattle liver extracting solution, protein sugar, citric acid, distilled water, wherein said milk cattle liver extracting solution can be made by following method: at first with the rubbing of milk cattle liver, homogenate, temperature is controlled at 70～95 ℃ then, time is controlled at the homogenate of 5～20 minutes scope internal heating, serosity cooling back centrifugalize, it is standby to get supernatant liquid filtering, and the content of each component is as follows in 1000 milliliters of healthful oral liquid for liver that it is characterized in that preparing:
200～500 milliliters of milk cattle liver extracting solution;
Protein sugar 0.5～1 gram;
Citric acid 1～4 gram;
Distilled water surplus.
According to the Traditional Chinese medical theory of motherland " dirty with dirty benefit ", it is primary raw material that the present invention adopts the milk cattle liver, is equipped with adjuvants such as protein sugar, citric acid again.Protein sugar is a kind of novel nutritive sweetener, and diabetics is not had any harmful effect, the pH value of citric acid scalable oral liquid.Adjuvant among the present invention mainly plays the seasoning effect, makes this oral liquid mouthfeel good, has the sweet taste of fragrance.
Because newborn milk cattle do not take food, therefore do not carry out metabolism, be primary raw material so milk cattle liver of the present invention preferably adopts the milk cattle liver in the birth 24 hours, to reach the nourishing liver, to promote hepatocellular regeneration better.
Primary raw material milk cattle liver extracting solution of the present invention also can adopt the liver nose part of Hepar Sus domestica to replace the liver of milk cattle, also can adopt 60% Hepar Sus domestica point and 40% milk cattle liver mixture extracting solution, is equipped with above-mentioned adjuvant again and makes.
In order to make this oral liquid meet popular taste, can in this oral liquid, add an amount of essence, also can add strawberry essence to improve the mouthfeel of oral liquid.
Preparation method of the present invention is divided two steps, at first is the preparation of milk cattle liver extracting solution, is the preparation of mixed liquor then.
The preparation of A, milk cattle liver extracting solution: liver is removed fat, fascia and blood vessel, connective tissue, rub then, reuse colloid mill (refiner) homogenate, again homogenate is heated, temperature is controlled at 76～95 ℃, time was controlled at 5-20 minute, and with the homogenate cooling back centrifugalize after the heating, it is standby to get supernatant liquid filtering after centrifugal.When the Hepar Sus domestica point that adopts 60% is produced extracting solution with 40% milk cattle liver mixture, equally also fat, fascia and blood vessel, the connective tissue of pig point should be removed, reuse is produced milk cattle liver extracting solution same process and is produced Hepar Sus domestica point extracting solution, makes Hepar Sus domestica point and milk cattle liver mixture extracting solution after both extracting solution is mixed according to the above ratio.
B. the preparation of mixed liquor: in filtering good supernatant, add protein sugar and citric acid and distilled water, through aseptic filtration, add by 1000 milliliters of these oral liquids of preparation that protein sugar 0.5-1 gram, citric acid 1-4 restrain, surplus is a distilled water in the 200-500 milliliter supernatant, the membrane material of aseptic filtration system adopts minimum to protein adsorption, and the biological activity that can effectively keep product, and the filtration system that the rate of filtration is fast, treating capacity is big can be distributed into finished product after the aseptic filtration.
For the effect that guarantees to heat, the present invention adopts hydronic method, prevents hot-spot, and heating and temperature control can not be above 95 ℃ at 70-95 ℃.
The freezing centrifugal method of high speed is adopted in homogenate cooling back centrifugalize of the present invention, and to guarantee isolating effect, centrifuge per minute 16000 changes, and temperature is controlled at 0-5 ℃.
The present invention contains highly active liver regeneration-stimulating factor.Evidence HSS is that a class has the material that specificity promotes liver cell regeneration, can be divided into macromolecule (＞110000), middle molecular weight (2-6 ten thousand), small-molecular weight (＜2 ten thousand).It is that mixing composition below 30,000 is as extracting solution that the present invention adopts membrane separation technique to select molecular weight in prepared, thereby strengthened the activity of the concentration HSS of unit, and the refreshing liquid of strong liver is that to select the liver of new life (in 24 hours) milk cattle for use be raw material in selection, extract refining forming through modern biochemical technology, contain the health product of high activity HSS.Extraction process is easy, adopt advanced colloid mill loop heating system, reach biological extraction technology such as high speed frozen centrifugation, the biological characteristics that has kept this product preferably, in the aseptic filtration process, we have selected the Millidisk of state-of-the-art in the world U.S. Millipore company to filter system, the membrane material of this filtration system is Durapore, and to the minimum effective HSS that kept of proteic absorption, and the rate of filtration is fast, treating capacity is big, be that other aseptic filtration mode is incomparable, the milk cattle liver of not taking food in newborn 24 hours does not pass through the metabolism detoxification processes, contains abundant originality HSS, active high, short liver growth effect is strong.Also contain the aminoacid and the various trace elements (seeing attached list) of 18 kinds of needed by human body in this oral liquid, can promote hepatic cell growth and regeneration, strengthen the function of detoxification of liver, hepatic injury is had prevention and repair; Prevent the formation of liver cirrhosis; And the alcoholic liver disease that long-term insobriety causes also there is an effect of prevention, adjusting, nutrition.This oral liquid good mouthfeel, safety non-toxic has fragrant sweet taste, and diabetics is not had any harmful effect.
Preparation technology of the present invention is easy, adopts advanced colloid mill, and the serosity biotechnology such as heating and high speed frozen centrifugation that circulate can keep the biological activity of this oral liquid.That the membrane material of aseptic filtration system in addition, adopts is minimum to protein adsorption, also effectively keep the biological activity of this oral liquid.The oral liquid of this method preparation can be collected the active substance of the different molecular weight of biologically active, can guarantee that this oral liquid has higher biological activity under lower concentration.
The embodiment of the invention 1:
Behind the degrease of newborn milk cattle liver, fascia and blood vessel, connective tissue, through rub, homogenate, homogenate circulated is heated to 90 ℃, heated 5 minutes, centrifugalize is carried out in cooling then, gets 250 milliliters of supernatant after the centrifugalize, after the filtration, add 0.55 gram protein sugar and 1.2 gram citric acids, surplus is a distilled water, is mixed with 1000 milliliters, after sterilization system filters, be distributed into finished product then.
The embodiment of the invention 2:
Processing step is with embodiment 1, and the circulation heating and temperature control heated 15 minutes at 85 ℃, and supernatant is got 300 milliliters, adds protein sugar 0.7 gram, citric acid 2.0 grams, and surplus is a distilled water, is mixed with 1000 milliliters.
The embodiment of the invention 3:
The Hepar Sus domestica point is rubbed (by 60% Hepar Sus domestica point, 40% milk cattle liver mixed) with the milk cattle liver, and its processing step is with embodiment 1, supernatant is got 450 milliliters, adds 0.9 gram protein sugar, 0.8 gram citric acid, surplus is a distilled water, prepares 1000 milliliters of these oral liquids.
Subordinate list
| Analysis project | Analysis result mg/l | Analysis project | Analysis result mg/l | Trace element |
| Asparatate threonine serine glutamic acid glycine alanine cystine valine methionine | 16.67 10.77 22.25 36.65 40.98 27.61 5.80 56.12 19.00 | Isoleucine leucine tryptophan tyrosine phenylalanine arginine lysine histidine proline | 15.64 15.28 22.37 120.43 14.92 24.89 3.47 2.81 17.43 | Zn0.002mg/ml Se＜0.0005mg/ml |
Claims (8)
1, a kind of healthful oral liquid for liver, it mainly is made up of milk cattle liver extracting solution, protein sugar, citric acid, distilled water, wherein said milk cattle liver extracting solution can be made by following method: at first with the rubbing of milk cattle liver, homogenate, temperature is controlled at 70～95 ℃ then, time is controlled at the homogenate of 5～20 minutes scope internal heating, serosity cooling back centrifugalize, it is standby to get supernatant liquid filtering, it is characterized in that the content of each component in 1000 milliliters of prepared healthful oral liquid for liver is as follows:
200～500 milliliters of milk cattle liver extracting solution;
Protein sugar 0.5～1 gram;
Citric acid 1～4 gram;
Distilled water surplus.
2, oral liquid as claimed in claim 1 is characterized in that said milk cattle liver is the milk cattle liver of birth in 24 hours.
3, oral liquid as claimed in claim 1 is characterized in that said milk cattle liver extracting solution also can adopt 60% Hepar Sus domestica point and 40% milk cattle liver mixture extracting solution.
4, oral liquid as claimed in claim 1 or 2 is characterized in that also can adding spice in the said oral liquid.
5, oral liquid as claimed in claim 4 is characterized in that said spice is strawberry essence.
6, a kind of method that is used to prepare the described oral liquid of claim 1, its processing step is as follows:
The preparation of A, milk cattle liver extracting solution: at first with liver rubbing, homogenate, with the homogenate heating, temperature is controlled at 70～95 ℃ then, 5～20 minutes time, and serosity cooling back centrifugalize, it is standby to get supernatant liquid filtering;
The preparation of B, mixed liquor: add protein sugar and citric acid, distilled water in the supernatant after filtering, adds in 200～500 milliliters of supernatant by 1000 milliliters of oral liquids of preparation that protein sugar 0.5～1 gram, citric acid 1～4 restrain, surplus is a distilled water, after aseptic filtration, promptly make this oral liquid.
7, method as claimed in claim 6 is characterized in that said homogenate adds the heating of thermal recovery flow circuit.
8, method as claimed in claim 6 is characterized in that serosity cools off the back centrifugalize and takes 16000 rev/mins of high speed frozen centrifugations, and temperature is controlled at 0～5 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN96119577A CN1069198C (en) | 1996-11-22 | 1996-11-22 | Oral liquid for strengthening liver and preparaing process thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN96119577A CN1069198C (en) | 1996-11-22 | 1996-11-22 | Oral liquid for strengthening liver and preparaing process thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1183282A CN1183282A (en) | 1998-06-03 |
| CN1069198C true CN1069198C (en) | 2001-08-08 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN96119577A Expired - Fee Related CN1069198C (en) | 1996-11-22 | 1996-11-22 | Oral liquid for strengthening liver and preparaing process thereof |
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| CN (1) | CN1069198C (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109568261A (en) * | 2019-01-04 | 2019-04-05 | 湖南海济药业有限公司 | A kind of polypeptide oral liquor and preparation method thereof of prevention and alleviation chemical damage |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1089852A (en) * | 1993-01-21 | 1994-07-27 | 陈玉春 | The natural biological oral liquid that contains the bioactie agent nucleotide polypeptide type |
| CN1096053A (en) * | 1993-12-16 | 1994-12-07 | 重庆医科大学病毒性肝炎研究所 | The preparation method of low molecular weight hepatic cells growth hormone |
-
1996
- 1996-11-22 CN CN96119577A patent/CN1069198C/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1089852A (en) * | 1993-01-21 | 1994-07-27 | 陈玉春 | The natural biological oral liquid that contains the bioactie agent nucleotide polypeptide type |
| CN1096053A (en) * | 1993-12-16 | 1994-12-07 | 重庆医科大学病毒性肝炎研究所 | The preparation method of low molecular weight hepatic cells growth hormone |
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| Publication number | Publication date |
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| CN1183282A (en) | 1998-06-03 |
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