CN108456613A - A kind of health care's wine and its preparation method - Google Patents

A kind of health care's wine and its preparation method Download PDF

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Publication number
CN108456613A
CN108456613A CN201810723515.2A CN201810723515A CN108456613A CN 108456613 A CN108456613 A CN 108456613A CN 201810723515 A CN201810723515 A CN 201810723515A CN 108456613 A CN108456613 A CN 108456613A
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parts
wine
health care
radix
fructus
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Inventor
朱兆云
王京昆
范孝开
游�燕
王恒禹
郭冬梅
余汪平
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Yunnan Pharmaceutical Institute
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Yunnan Pharmaceutical Institute
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12GWINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
    • C12G3/00Preparation of other alcoholic beverages
    • C12G3/04Preparation of other alcoholic beverages by mixing, e.g. for preparation of liqueurs
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/06Fungi, e.g. yeasts
    • A61K36/07Basidiomycota, e.g. Cryptococcus
    • A61K36/076Poria
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/22Anacardiaceae (Sumac family), e.g. smoketree, sumac or poison oak
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/23Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
    • A61K36/232Angelica
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/25Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
    • A61K36/258Panax (ginseng)
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/34Campanulaceae (Bellflower family)
    • A61K36/346Platycodon
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/40Cornaceae (Dogwood family)
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/46Eucommiaceae (Eucommia family), e.g. hardy rubber tree
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/48Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A61K36/481Astragalus (milkvetch)
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/48Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A61K36/484Glycyrrhiza (licorice)
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/48Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A61K36/488Pueraria (kudzu)
    • AHUMAN NECESSITIES
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    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/54Lauraceae (Laurel family), e.g. cinnamon or sassafras
    • AHUMAN NECESSITIES
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    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/61Myrtaceae (Myrtle family), e.g. teatree or eucalyptus
    • AHUMAN NECESSITIES
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    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/68Plantaginaceae (Plantain Family)
    • AHUMAN NECESSITIES
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    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/73Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
    • A61K36/732Chaenomeles, e.g. flowering quince
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/80Scrophulariaceae (Figwort family)
    • A61K36/804Rehmannia
    • AHUMAN NECESSITIES
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    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/81Solanaceae (Potato family), e.g. tobacco, nightshade, tomato, belladonna, capsicum or jimsonweed
    • A61K36/815Lycium (desert-thorn)
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/88Liliopsida (monocotyledons)
    • A61K36/896Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
    • A61K36/8965Asparagus, e.g. garden asparagus or asparagus fern
    • AHUMAN NECESSITIES
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    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/88Liliopsida (monocotyledons)
    • A61K36/898Orchidaceae (Orchid family)
    • A61K36/8984Dendrobium
    • AHUMAN NECESSITIES
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    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/88Liliopsida (monocotyledons)
    • A61K36/906Zingiberaceae (Ginger family)
    • A61K36/9064Amomum, e.g. round cardamom
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    • A61K36/88Liliopsida (monocotyledons)
    • A61K36/906Zingiberaceae (Ginger family)
    • A61K36/9068Zingiber, e.g. garden ginger
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00Medicinal preparations characterised by special physical form
    • A61K9/0087Galenical forms not covered by A61K9/02 - A61K9/7023
    • A61K9/0095Drinks; Beverages; Syrups; Compositions for reconstitution thereof, e.g. powders or tablets to be dispersed in a glass of water; Veterinary drenches
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    • A61K2236/30Extraction of the material
    • A61K2236/33Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/333Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH

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Abstract

A kind of health care's wine and its preparation method, the wine are made by the raw material of following parts by weight:1 20 parts of fresh Radix Notoginseng, 0 18 parts of ginseng, 0 24 parts of Radix Angelicae Sinensis, 0 30 parts of pueraria lobata, 0 30 parts of prepared rehmannia root, 0 24 parts of the fruit of Chinese wolfberry, 0 30 parts of PORIA ALBA, 0 60 parts of Radix Astragali, 0 18 parts of Radix Glycyrrhizae, 0 12 parts of fructus amomi, 0 10 parts of Chinese cassia tree, 06 parts of cloves, 0 18 parts of Cortex Eucommiae, 0 30 parts of plantain seed, 0 24 parts of asparagus fern, 0 24 parts of Fructus Corni, 0 18 parts of rhizoma zingiberis, 0 18 parts of campanulaceae, 0 24 parts of the stem of noble dendrobium, 06 parts of fructus choerospondiatis, 0 18 parts of pawpaw, 500 1000 parts of base liquor.By slice or the medicinal material crushed, the room temperature in the grain neutral spirit of 30-60%vol is protected from light brewed 30 90 days of sealing, is obtained after filtering, rectification degree and secondary filter.Present invention process is simple, and immunity of organisms can be enhanced, slow down aging by drinking this wine in right amount.

Description

A kind of health care's wine and its preparation method
Technical field
The invention belongs to technical field of health care food, and in particular to a kind of health care's wine with strengthen immunity function And its preparation method.
Background technology
Social progress and improvement of living standard also bring one while bringing many benefits to the health of the mankind A little new unfavorable factors, such as excessively fierce, nervous fast pace life, increasingly increased operating pressure are brought more to people Stress causes sub-health population to increase;Furthermore since human factor influences:Such as losing weight, improper, rest is insufficient, for a long time It does arduous work, eat and drink immoderately, drink beyond one's capacity, can all cause bodily fuctions' overload operation, vegetative nerve functional disturbance, Internal system functional disturbance causes the immunity of body to reduce.
Immune function of human body is low, and human body is made to be difficult to resist the pathogenic microorganisms invasion of bacterium, virus, fungi etc., it is difficult to The dead cell of internal aging is removed in time, and is difficult to find and eliminate the cell to morph in vivo in time, is prevented pernicious The generation of tumour.Human body body's immunity declines as a kind of stealthy killer endangering modern's health, has seriously affected The work and life of people.Because the invention it is a kind of can strengthen immunity, improve human body prevent disease instinct health food tool There are certain market prospects.
Radix Notoginseng:For the dry root and rhizome of panax araliaceae plant Panaxnotoginseng (Burk.) F.H.Chen.Three It is seven warm-natured, bitter, nontoxic, there is anti-oxidant, anti-aging, anti-inflammatory, protect liver cholagogic, hemostasis and anticoagulation, resisting coronary heart disease, the anti-rhythm of the heart Not normal, expansion blood vessel and reducing blood pressure and blood fat and other effects, also inhibit the effect of cancer cell diffusion.Studies have shown that in Radix Notoginseng Saponin(e, polysaccharide and aminoacid ingredient have the function of anti-aging, antifatigue, strengthen immunity.Notoginseng polysaccharide can dramatically increase macrophage Cell phagocytic activity increases lysozyme content in serum, and the leucocyte of low leucocyte disease patient is made to restore normal level, and to just Normal leucocyte has the function of adjusting immune function of human body without influence.
Ginseng:For the root of Araliaceae ginseng Panax ginseng C.A..Ginseng writes the laudatory title of " King of Herbs ", It is a kind of nutritive value and all very high plant of drug value, the superfine product of " life is mended in enriching yin, is strengthened the body resistance to consolidate the constitution " is described as by medical field.People Ginseng contains abundant Germanium, and germanium is known as " magical element " by medical field reputation, has fabulous activity, ginseng is not not cold dry, always It is few suitable, it is suitble to various constitutions edible, can fully and effectively adjusts the equilibrium between yin and yang of human body cell, improves cell metabolism level, Enhance body function, improve immunity, prevent various diseases, restores human body and respectively organize tract function.Long-term consumption can make Human body is in optimum, full of vitality, and U.S. face, which is supported, to be held, and is promoted longevity.
In addition, Radix Angelicae Sinensis, pueraria lobata, prepared rehmannia root, the fruit of Chinese wolfberry, PORIA ALBA, Radix Astragali, Radix Glycyrrhizae, fructus amomi, Chinese cassia tree, cloves, Cortex Eucommiae, vehicle Preceding son, asparagus fern, Fructus Corni, rhizoma zingiberis, campanulaceae, the stem of noble dendrobium, fructus choerospondiatis, pawpaw etc. are also enriched in the natural activity of various saponin(es, flavones, polysaccharide Ingredient has effects that good anti-oxidant, strengthen immunity.
The composition of the above medicinal material with grain neutral spirit it is brewed made of health care's wine have enhancing immunity of organisms, antioxygen The effects that changing and slowing down aging.
Invention content
The purpose of the present invention is to provide a kind of health with strengthen immunity function good in taste, easy to make to support Raw wine and its preparation method.The present invention uses integration of drinking and medicinal herbs and can be used for the medicinal material taste raw material of health food, through 30-60%vol Soaking in Chinese liquor extract its essential ingredient, dilute, filter, process for preparation does not introduce any poisonous and harmful chemical reagent, institute With to human body without any toxicity and side effects.It drinks in right amount, there is antifatigue, strengthen immunity, improve the guarantors such as human body prevention disease instinct Health-care function.
The present invention used scheme be:A kind of health care's wine, is made by the raw material of following parts by weight:Fresh Radix Notoginseng 1- 20 parts, 0-18 parts of ginseng, 0-24 parts of Radix Angelicae Sinensis, 0-30 parts of pueraria lobata, 0-30 parts of prepared rehmannia root, 0-24 parts of the fruit of Chinese wolfberry, 0-30 parts of PORIA ALBA, 0-60 parts of Radix Astragali, 0-18 parts of Radix Glycyrrhizae, 0-12 parts of fructus amomi, 0-10 parts of Chinese cassia tree, 0-6 parts of cloves, 0-18 parts of Cortex Eucommiae, plantain seed 0-30 Part, 0-24 parts of asparagus fern, 0-24 parts of Fructus Corni, 0-18 parts of rhizoma zingiberis, 0-18 parts of campanulaceae, 0-24 parts of the stem of noble dendrobium, 0-6 parts of fructus choerospondiatis, pawpaw 0- 18 parts, 500-1000 parts of base liquor.
Preferably, it is made by the raw material of following parts by weight:Fresh Radix Notoginseng 3-9 parts, 3-9 parts of ginseng, 0-12 parts of Radix Angelicae Sinensis, pueraria lobata 0- 15 parts, 9-15 parts of prepared rehmannia root, 6-12 parts of the fruit of Chinese wolfberry, 0-15 parts of PORIA ALBA, 9-30 parts of Radix Astragali, 2-9 parts of Radix Glycyrrhizae, 3-6 parts of fructus amomi, meat Osmanthus 1-4.5 parts, 1-3 parts of cloves, 0-9 parts of Cortex Eucommiae, 0-15 parts of plantain seed, 0-12 parts of asparagus fern, 0-12 parts of Fructus Corni, 3-9 parts of rhizoma zingiberis, 3-9 parts of campanulaceae, 3-12 parts of the stem of noble dendrobium, 1.5-2.5 parts of fructus choerospondiatis, 6-9 parts of pawpaw, 500-1000 parts of base liquor.
The base liquor is the grain neutral spirit of 30-60%vol.
A kind of preparation method of health care's wine includes the following steps:
(1) it stocks up:Each component is weighed by formula rate;
(2) feedstock processing:By the medicinal material weighed suitably crushing or slicing treatment;
(3) brewed:Medicinal material is soaked in base liquor, after stirring and evenly mixing, room temperature is protected from light brewed 30-90 days of sealing;
(4) it filters:Wine liquid slag filtration after will be brewed;
(5) it modulates:It is 15-38%vol that filtered wine liquid is adjusted alcoholic strength with distilled water, sealing and standing 1- after mixing 3 days;
(6) secondary filter:Modulated wine liquid is removed sediment through secondary filter to dispense to obtain assembled alcoholic drinks.
The appropriate present invention that drinks can alleviate physical fatigue, enhancing immunity of organisms, slow down aging, and be a the aged,middle aged person and young man's four seasons All suitable health care's wine.
Specific implementation mode
The present invention is described in detail with reference to embodiments.
Embodiment 1:
(1) 3 parts of the fresh Radix Notoginseng cleaned and dried, 3 parts of ginseng, 3 parts of Radix Angelicae Sinensis, 5 parts of pueraria lobata, 9 parts of prepared rehmannia root, matrimony vine are weighed 6 parts of son, 2 parts of PORIA ALBA, 9 parts of Radix Astragali, 5 parts of Radix Glycyrrhizae, 3 parts of fructus amomi, 1 part of Chinese cassia tree, 1 part of cloves, 3 parts of Cortex Eucommiae, 3 parts of plantain seed, day 4 parts of winter, 4 parts of Fructus Corni, 3 parts of rhizoma zingiberis, 3 parts of campanulaceae, 4 parts of the stem of noble dendrobium, 2 parts of fructus choerospondiatis, 6 parts of pawpaw;
(2) fresh Radix Notoginseng, ginseng are cut into the sheet of thickness about 0.2cm respectively;
(3) sealing is protected from light immersion 30 after being stirred and evenly mixed in wine vat of the above raw material input equipped with 500 portions of 53 degree of liquor made from sorghums It;
(4) the medicinal material residue in wine vat is first fished for, wine liquid removes remaining medicinal material residue through screen filtration;
(5) wine liquid alcoholic strength is adjusted to 37 degree with distilled water, is sealed after mixing and is placed at room temperature for 2 days;
(6) wine liquid after standing is filtered to remove sediment and dispensed through filter paper and obtain the health with plurality of health care functions Health preserving wine.
Embodiment 2:
(1) 9 parts of the fresh Radix Notoginseng cleaned and dried, 9 parts of ginseng, 12 parts of Radix Angelicae Sinensis, 15 parts of pueraria lobata, 15 parts of prepared rehmannia root, Chinese holly are weighed 12 parts of matrimony vine, 15 parts of PORIA ALBA, 30 parts of Radix Astragali, 9 parts of Radix Glycyrrhizae, 6 parts of fructus amomi, 4.5 parts of Chinese cassia tree, 3 parts of cloves, 9 parts of Cortex Eucommiae, plantain seed 15 parts, 12 parts of asparagus fern, 12 parts of Fructus Corni, 9 parts of rhizoma zingiberis, 9 parts of campanulaceae, 12 parts of the stem of noble dendrobium, 2.5 parts of fructus choerospondiatis, 9 parts of pawpaw;
(2) fresh Radix Notoginseng, ginseng are cut into the sheet of thickness about 0.2cm respectively;
(3) sealing is protected from light immersion after being stirred and evenly mixed in wine vat of the above raw material input equipped with 800 portions of 30%vol liquor made from sorghums 90 days;
(4) the medicinal material residue in wine vat is first fished for, wine liquid removes remaining medicinal material residue through screen filtration;
(5) wine liquid alcoholic strength is adjusted to 18%vol with distilled water, is sealed after mixing and is placed at room temperature for 3 days;
(6) wine liquid after standing is filtered to remove sediment and dispensed through filter paper and obtain the health with plurality of health care functions Health preserving wine.
Embodiment 3:
1. pair embodiment 2 carries out antibody-producting cell detection (Jerne improves slide methods)
1.1 experiment material
(a) raw material:Brown color liquid is voluntarily provided by this laboratory;
(b) experiment mice:Female Balb/C mouse, 18-22g are purchased from Shanghai Si Laike Experimental Animal Centers;It is moulded with sterilizing Expect that mouse cage is fed, free water, the control of animal house temperature is at 20-25 DEG C, humidity 55-65%, illumination 10-12h/d, mouse cage one week It replaces 2-3 times;
(c) carbon dioxide incubator, water bath with thermostatic control, centrifuge, surgical instrument, glass frame, SRBC (hundred base of Zhengzhou biology), Complement (guinea pig serum) (hundred base of Zhengzhou biology), Hank ' s liquid (Hyclone), agarose.
1.2 experimental method
(a) experiment each group is divided into 10,20 and 40mL/kg, tri- dosage groups and a blank control group, continuous gavage 4 weeks;
(b) after dissolving by heating surface layer culture medium (1g agaroses add distilled water to 100mL), 45~50 DEG C of water-baths is put and are protected Temperature is mixed with Hank ' the s liquid of equivalent pH7.2~7.4,2 times of concentration, dispenses small test tube, often pipe 0.5mL, then 50 are added into pipe μ L10%SRBC (v/v, with normal saline), 20 μ L splenocyte suspensions (5 × 106A/mL), rapid mixing is poured into On the slide of brush agarose thin layer, parallel plate is done, after agar solidification, slide level is buckled and is placed on horse, titanium dioxide is put into It is incubated 1~1.5h in carbon incubator, then uses the complement (1 of normal saline dilution:8) it is added to glass frame groove, continues temperature After educating overnight, hemolysis plaque number is counted.
1. health care's wine of table is to antibody-producting cell detection
At the end of experiment, three dosage group plaque numbers are all remarkably higher than control group, can determine that this experimental result positive.
Embodiment 4:
2. pair embodiment 2 carries out the measurement (Hemagglutination Method) of serum hemolysin
2.1 experiment material
(a) raw material:Brown color liquid is voluntarily provided by this laboratory;
(b) experiment mice:Female Balb/C mouse, 18-22g are purchased from Shanghai Si Laike Experimental Animal Centers;It is moulded with sterilizing Expect that mouse cage is fed, free water, the control of animal house temperature is at 20-25 DEG C, humidity 55-65%, illumination 10-12h/d, mouse cage one week It replaces 2-3 times;
(c) SRBC, physiological saline, Microhemagglutination experimental plate (Haimen Tian Yan experiment equipments business department), centrifuge.
2.2 experimental method
(a) experiment each group is divided into 10,20 and 40mL/kg, tri- dosage groups and a blank control group, continuous gavage 4 weeks;
(b) serum of different dilutions is respectively placed in Microhemagglutination experimental plate by serum doubling dilution with physiological saline It is interior, per 100 μ L of hole, the SRBC suspensions of 100 μ L 0.5% (v/v) are added, mixing is packed into the square position of moistening and is capped, in 37 DEG C incubation 3h or overnight, observes hemagglutination degree.
Measurement of the 2. health care's wine of table to serum hemolysin
At the end of experiment, the high, normal, basic three dosage group antibody levels of health care's wine are significantly higher than control group, can determine that this Item experimental result is positive.

Claims (4)

1. a kind of health care's wine, which is characterized in that the raw material weight proportioning of soup processed of health care's wine is:Fresh Radix Notoginseng 1-20 parts, 0-18 parts of ginseng, 0-24 parts of Radix Angelicae Sinensis, 0-30 parts of pueraria lobata, 0-30 parts of prepared rehmannia root, 0-24 parts of the fruit of Chinese wolfberry, 0-30 parts of PORIA ALBA, Radix Astragali 0-60 parts, 0-18 parts of Radix Glycyrrhizae, 0-12 parts of fructus amomi, 0-10 parts of Chinese cassia tree, 0-6 parts of cloves, 0-18 parts of Cortex Eucommiae, 0-30 parts of plantain seed, day Winter 0-24 part, 0-24 parts of Fructus Corni, 0-18 parts of rhizoma zingiberis, 0-18 parts of campanulaceae, 0-24 parts of the stem of noble dendrobium, 0-6 parts of fructus choerospondiatis, 0-18 parts of pawpaw, 500-1000 parts of base liquor.
2. a kind of health care's wine according to claim 1, which is characterized in that its preferred raw material weight proportioning of soup processed is:It is fresh 9 parts of Radix Notoginseng, 9 parts of ginseng, 12 parts of Radix Angelicae Sinensis, 15 parts of pueraria lobata, 15 parts of prepared rehmannia root, 12 parts of the fruit of Chinese wolfberry, 15 parts of PORIA ALBA, 30 parts of Radix Astragali, 9 parts of Radix Glycyrrhizae, 6 parts of fructus amomi, 4.5 parts of Chinese cassia tree, 3 parts of cloves, 9 parts of Cortex Eucommiae, 15 parts of plantain seed, 12 parts of asparagus fern, 12 parts of Fructus Corni, rhizoma zingiberis 9 parts, 9 parts of campanulaceae, 12 parts of the stem of noble dendrobium, 2.5 parts of fructus choerospondiatis, 9 parts of pawpaw.
3. a kind of health care's wine according to claim 1 or 2, which is characterized in that the preparation method of health care's wine Processing step includes:
(1)Weigh that the main materials medicinal materials such as cleaned fresh Radix Notoginseng are sliced or pulverization process in proportion;
(2)By treated, medicinal material mixture is soaked in base liquor, and after stirring and evenly mixing, room temperature is protected from light brewed 30-90 days of sealing;
(3)Wine liquid slag filtration after will be brewed;
(4)Filtered wine liquid is diluted into wine degree with distilled water, sealing and standing 1-3 days after mixing;
(5)Modulated wine liquid is removed sediment through secondary filter to dispense to obtain assembled alcoholic drinks.
4. a kind of health care's wine according to claim 1-3, it is characterised in that:It can be made into wine degree after distilled water dilutes For the health liquor of 15-38%vol.
CN201810723515.2A 2018-07-04 2018-07-04 A kind of health care's wine and its preparation method Pending CN108456613A (en)

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Application publication date: 20180828