CN106916749A - The cultural method and culture apparatus of a kind of nostoc - Google Patents
The cultural method and culture apparatus of a kind of nostoc Download PDFInfo
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Abstract
The invention provides a kind of nostoc cultural method and culture apparatus, belong to microdisk electrode industrial technical field.The cultural method be by nostoc in Thin cell layer liquid quiescent culture, enable the microalgae directly using Thin cell layer liquid Surface absorption CO2.The culture apparatus is stacked microalgae Thin cell layer device.The inventive method and device can increase the yield of biomass of unit area light source.By thin layer, static type culture, can save for energy consumptions such as carbon, stirrings, unit cost is effectively reduced.
Description
Technical field
The invention belongs to algae bio culture field, it is related to the cultural method of nostoc and culture apparatus.
Background technology
Nostoc (Nostoc sphaeroides K ü tzing) is a kind of many cells blue-green algae, belongs to nostoc, and scientific name is the spherical nostoc of plan.Nostoc is a kind of edible blue-green algae, road dogmatics scholar, the name and its experience of physician and alchemist Ge Hong of Yin Jinchao and name, it is also recorded in Compendium of Materia Medica the diseases such as treatment yctalopia, prolapse of the anus.Wild Nostoc also has rich in protein, exocellular polysaccharide and various trace elements, its protein content is up to 52% and its amino acid classes is complete, also (polysaccharide of wherein bioactivity is up to 8%-12%) of total sugar content 20% or so, therefore it can be widely applied to the fields such as food, medicine and health care；Its polysaccharide and aliphatic acid are studied now with many scholars, is found the effects such as its polysaccharide has antioxidation activity, antiviral and antibacterial activity and enhancing immunity of organisms, its aliphatic acid is also had certain therapeutic effect to heart disease.Because of its special growing environment, so Wild Nostoc is only existed in the rice terrace in a small number of mountain areas of China.But the chemical fertilizer brought by the development of modern agriculture and indiscriminate use of pesticide, and cause the yield of Wild Nostoc drastically to reduce, the application requirement of people has been unable to reach, therefore artificial culture is inexorable trend.For artificial culture nostoc, how to increase nostoc biomass, the cost for how reducing its culture is its Mirae Corp., the main barrier of the marketization.
The cultural method of current nostoc is mainly liquid-immersed method, and typical bioreactor has open raceway pond and glass plate formula bioreactor.Immersion method is that algae is immersed in substantial amounts of nutrient solution, by mixing plant or recycle unit or aerator, nutrient solution is continuously in shuttling movement state, each nostoc cell is equably received illumination, is absorbed CO2And nostoc cell is not deposited on reactor bottom.But, it is exactly that energy consumption is big, biomass is low (dry cell weight of nostoc is only 0.4g/L) that the cultural method and device have common drawback, and light utilization ratio is low, low space utilization, water consumption are big, the shearing force for being easy to be either mechanically agitated part damages eucaryotic cell structure (during high-speed stirred), wherein with energy consumption it is big, light utilization ratio is low, water consumption is more, low space utilization and be easy to be stirred blade damage eucaryotic cell structure as its major drawbacks.
Therefore, traditional immersion method culture microalgae, its unit cost height is the problem recognized by industry, is also the problem of industrialization urgent need to resolve.
The content of the invention
First purpose of the invention is to provide a kind of nostoc cultural method, is used to solve the problems, such as that unit cost present in current nostoc culture is high.
Second object of the present invention is to provide a kind of culture apparatus for being adapted to the cultural method.
To achieve the above object, present invention firstly provides a kind of microalgae culture method, its be by microalgae in Thin cell layer liquid quiescent culture, enable the microalgae directly using Thin cell layer liquid Surface absorption CO2。
Wherein, the thickness of the nutrient solution can be 1mm~20mm, and preferably the thickness of nutrient solution is 2mm~10mm.
Based on this, the present invention further provides a kind of cultural method of nostoc, its be by nostoc in Thin cell layer liquid quiescent culture, enable the nostoc directly using Thin cell layer liquid Surface absorption CO2.Wherein, the thickness of the nutrient solution is 1~4 times of nostoc particle size range, and it is 0.4mm or so that nostoc does the particle diameter planted, and the diameter of the fresh grain of nostoc is about between 1.0mm~5mm.The thickness of the nutrient solution can be 1mm~20mm, and preferably the thickness of nutrient solution is 2mm~10mm.
Wherein, the environment temperature of the quiescent culture can be 20-28 DEG C.
Wherein, the luminous intensity of the quiescent culture can be 2~526 μm of olm-2·s-1, more preferably 16.9~274.6 μm olm-2·s-1。
Wherein, the culture medium of the quiescent culture can be the BG11 fluid nutrient mediums without nitrogen.
Wherein, the ambient humidity range of the quiescent culture can be 80-99%.
Wherein, nostoc inoculum concentration can be 0.1-0.9g/L.
Further, the present invention provides a kind of stacked microalgae Thin cell layer device for being adapted to above-mentioned microalgae quiescent culture, its culture plate for including multiple stacked distributions, the culture plate includes base plate and around base plate and the closely coupled cofferdam of base plate, the lowest part in the cofferdam is highly 1mm-20mm, and a preset distance is spaced between neighbouring two culture plate.
Wherein, there is support member, support member makes to form described preset distance between neighbouring culture plate between neighbouring two culture plate.
Wherein, the support member can be connected with the cofferdam of neighbouring two culture plate or base plate respectively.
Support member can be a set of fixed support system, such as support frame or support column etc., culture plate be connected into culture plate matrix, or culture plate is connected into culture plate matrix using suspension system.For example the support member is support column in one embodiment of the present invention.
Wherein, the spacing between neighbouring two culture plates base plate and base plate is 5~30mm.
Wherein, the base plate of the culture plate and/or the material in cofferdam are preferably transparent material, including but not limited to following transparent material：Glass, GPPS, ABS, styrene-acrylonitrile, PVC, PMMA, polycarbonate or polystyrene.
Wherein, the material of the support member is preferably transparent or light transmissive material.
Wherein, the culture plate can be integrally formed.
Wherein, the culture plate can be integrally formed with the support member.
Wherein, the baseboard material of the culture plate can be plate glass, and the bank material of the culture plate can beGelatinBar.
Wherein, it is also provided with light source at least one side of the Thin cell layer device.
The present invention uses thin layer static culture method, because the thickness of nutrient solution is sufficiently small, therefore need not stir the operations that homogenize such as mixing, it is not required that to being passed through CO in nutrient solution2, remain able to ensure that frustule more can equably obtain illumination in nutrient solution, and being capable of gas exchanges, the CO required for obtaining frustule photosynthesis from the liquid level of nutrient solution2The O for suppressing is produced to photosynthesis with discharge2.Therefore, cultural method of the invention has saving energy consumption, reduces the advantage of toxigenic capacity.InventionPeople dashes forwardBroken traditional immersion cultural method carried out with Large Copacity vessel, the present invention is the processing mode for being firstly introduced thin layer, static culture, its essence is the one kind between immersion method and semidry methodIt is newCultural method.
Culture apparatus of the invention, it is possible to achieve high density be laminated, between every layer have gap, be available for light to propagate to larger distance, therefore, light can and in the range of can cultivate nostoc.So just directly increase the yield of biomass of unit area light source.Again because of thin layer, static type culture, therefore save for energy consumptions such as carbon, stirrings.
Brief description of the drawings
Figure 1It is a signal of culture apparatus of the present inventionFigure (Main viewFigure)。
Figure 2It is the culture plate signal of culture apparatus of the present inventionFigure。
Figure 3It is another signal of culture apparatus of the present inventionFigure (Side-lookingFigure)。
Figure 4AIt is the vertical view of one embodiment of the present inventionFigure, being cultivated under 1 ㎡ light sources with the culture piece that area is 120mm*60mm, light source 1m high, 1m wide, culture plate totally 7 row is vertical with light source.
Figure 4BIt is the raceway pond signal in traditional training methodFigure (OverlookFigure), raceway pond surface area is 2.5m2。
Figure 4CIt is goldfish pool reactor signal in traditional training methodFigure (It is three-dimensionalFigure), its length, width and height is respectively 55cm, 20cm and 45cm.
Figure 5It is nostoc culture of the present invention, the light intensity value and nostoc dry cell weight measured under light source difference near-far situation.
In figure：1 culture plate, 11 base plates, 12 cofferdam, 2 support members, 3 light sources.
Specific embodiment
With reference toAccompanying drawingThe present invention is described in detail, but should not be construed as limiting the invention.
The present invention provide nostoc cultural method, its be by nostoc in Thin cell layer liquid quiescent culture, enable the nostoc directly using Thin cell layer liquid Surface absorption CO2。
Nutrient solution thickness is advisable with being not higher than 20mm, preferably 1.5-10mm, more preferably 2~10mm, can be about 1mm, about 2mm, about 3mm, about about 4mm, 5mm, the scope between 7mm, 10mm or described arbitrary value.Nutrient solution thickness is excessive, and the CO for obtaining is interacted with air by nutrient solution surface2Then it is not enough to support the lasting culture of nostoc, and light energy losses are more, the algae kind below liquid level cannot absorb enough light intensity.In addition, nutrient solution thickness is too small, such as less than 0.3mm, on the one hand because nutrient solution is too thin, some parts for growing the larger fresh nostoc of particle diameter may be by exposed to influence growth outside nutrient solution, and the volatilization of moisture is unfavorable for culture, culture amount is reduced simultaneously, also uneconomical.
Certainly, above is being carried out based on conventional culture conditions.If by improving CO2The measures such as concentration, improvement illumination condition, the thickness of nutrient solution can increased.
Because the thickness of culture medium in the method for the present invention is sufficiently small so that nostoc cell can directly obtain photosynthesis from the liquid level of nutrient solution by gas exchanges required for CO2Photosynthesis of being rivals in a contest side by side produces the O for suppressing2, therefore aeration need not be carried out to nutrient solution.Furthermore, it is not necessary that the operation that homogenizes such as stirring mixing, remains able to ensure that the nostoc cell in nutrient solution more can equably obtain illumination.
Frond is harvested and drying is the important step of algae culture, and its energy consumption is big, and efficiency is low.However, the method according to the invention, using thin layer fluid nutrient medium static culture nostoc, when algae grows to maturation (typically about 8-12 days), culture medium is usually dissipated major part.Now, dry cell weight has been reached compared with ratios, is very beneficial for frond and is harvested, dries.In nutrient solution thickness range of the invention (1mm-10mm), the thickness of culture medium can be adjusted according to factors such as nostoc inoculum concentration, light intensity, humidity and temperature so that the amount of culture medium is available for algae to grow into the maturity period without having excessive having more than needed just.Further, since frond is constantly in Thin cell layer liquid, bacterium of mildewing is less susceptible to.
In some embodiments, the temperature for being used in the nostoc cultural method is 20-28 DEG C.In some embodiments, the light intensity for being used in the nostoc culture is 2-526 μm of olm-2·s-1.In some embodiments, the culture medium for being used in the nostoc culture is the BG11 fluid nutrient mediums without nitrogen.In some embodiments, the ambient humidity range in the nostoc culture is 80-99%.In some embodiments, in the nostoc culture, nostoc inoculum concentration can be 0.1-0.9g/L.
Nostoc cultural method of the invention by the way of thin layer static culture, its essence is the one kind between immersion method and semidry methodIt is newNostoc cultural method, as described above, having saving energy consumption, reduces toxigenic capacity, reduces mould, can effectively control the advantage of other enemy plankton explosion type pollution risks.
A kind of stacked nostoc Thin cell layer device for being adapted to above-mentioned nostoc quiescent culture that the present invention is provided, its culture plate for including multiple stacked distributions, the culture plate includes base plate and around base plate and the closely coupled cofferdam of base plate, the lowest part in the cofferdam is highly 1mm-20mm, and a preset distance is spaced between neighbouring two culture plate.
Due to using Thin cell layer, therefore the weight that culture plate is born is relatively low, therefore can be more frivolous, such that it is able to improve the transparency of culture plate, is conducive to penetrating for light.Also, due to there is gap between each layer culture plate, cofferdam is also transparent material, therefore light can laterally approach into above culture plate and bottom surface so that the nostoc light wherein cultivated is more fully and uniform, substantially increases the utilization ratio of light source.
The length of the culture plate for example can be 10cm-10m, and width for example can be 5cm-1m.Thickness can be 0.5mm~2.0mm, and according to the difference of material, thickness can be adjusted.It is each to cultivate in the vertical direction arranged stacked for no reason, maximize effective culture area of structure assembly, unit floor space.
The cofferdam can be perpendicular to base plate, or with base plate into a certain degree of inclination angle.For example, for the ease of harvesting nostoc, can be by least side or a part of cofferdam and the inclination angle (i.e. cofferdam inclines laterally) that base plate is in more than 90 °, such as in 135 ° of angles.Additionally, cofferdam and base plate binding site can be fillets, can so prevent from forming results dead angle or cleaning dead angle.
The height in cofferdam can be with identical, it is also possible to different.The distance that cofferdam is higher by base plate can be 1-20mm, preferably 2mm~10mm, can be about 1mm, about 2mm, about 3mm, about about 4mm, 5mm, the scope between 7mm, 10mm, or the arbitrary value.
Cofferdam and base plate can be fabricated separately be re-incorporated into together with or be integrally formed.These can make appropriate adjustment according to preparation difficulty and cost factor.
The base plate and bank material for forming culture plate can be glass or light transmittance plastics high, such as GPPS, transparent ABS, AS (styrene-acrylonitrile), PVC, PMMA (polymethyl methacrylate), PC (polycarbonate), PS (polystyrene) etc..Cofferdam can be the same or different with the material of base plate, for example, can use other transparent materials, such as adhesive tape, glass cement.
Spacing between each culture plate requires to determine according to light source arrangement, the culture of nostoc and results.For example, can be 5mm-30mm.
There is support member between neighbouring two culture plate, support member makes to form described preset distance between neighbouring culture plate.The support member can be connected with the cofferdam of neighbouring two culture plate or base plate respectively.Support member can be a set of fixed support system, such as support frame or support column etc., culture plate be connected into culture plate matrix, or culture plate is connected into culture plate matrix using suspension system.For example the support member is support column in certain embodiments of the present invention.In further embodiments, support member is not used, but uses a kind of support with several grids, the culture plate that these several plate glass are made is respectively rested on these supports, a determining deviation is kept between each culture plate.The support configuration has leveling system, can smoothly stand in ground, and each grid also is provided with leveling structure, can offset and miss because of the unhorizontal problem in the poor culture plate surface for causing.
The culture plate can be integrally formed with the support member, it is also possible to recombinant is manufactured separately to together.For example, support member is support column, support member can be fixedly mounted on the bottom of corresponding culture plate, or integrally formed mode, form the unit comprising culture plate and support member, the culture apparatus of recomposition stacking.
Additionally, the culture apparatus may also include the culture plate being laminated described in some rows, culture array is formed, so as to realize large-scale culture.
In addition, nostoc culture apparatus of the present invention can also further include nutrient solution instillation system, pipeline including instillation head, culture liquid case, connection instillation head and culture liquid case, the control circuit that control instillation is turned on and off, sensor of nutrient solution liquid level etc. in sensing culture plate, liquid level signal is sent to control circuit by sensor, and control circuit controls being turned on and off for instillation system according to liquid level signal.Certainly, the instillation system typically can not be used by the culture thickness and related condition of culture of initial setting up of the present invention.
When using nostoc culture apparatus culture nostoc of the present invention, the culture apparatus can be placed in the greenhouse with humidity control system, humidity control system and illumination system, temperature, humidity and illumination condition of relative constancy etc. are provided by greenhouse, the too fast volatilization of culture medium is avoided, nostoc is in suitable growing environment all the time.
Because the present invention is a kind of thin layer, static state, the training method of high density stacking between traditional immersion and half dry type, wherein the thickness of culture medium can according to the type of the algae of culture and life habit and environmental factor etc., based on experience value, calculated in advance and estimate, ambient humidity can manual control, make when nostoc culture is reached and harvested, dry cell weight proportion has been in of a relatively high scope, is harvested to the later stage and saves energy consumption.And algae even in very thin nutrient solution, is less susceptible to bacterium of mildewing, it is also possible to by enemy Environmental capacity in subrange in culture apparatus of the invention.
It is assumed that its thickness of each flat layer is 1.5mm, and the height of support column about 5~20mm, liquid thickness is 2.5mm；45~153 layers can be so stacked under 1M height spaces, if cultivating nostoc in each layer of cofferdam of flat board, the biomass that can be cultivated in 1m altitude ranges is considerable, and dry cell weight 1608g/m is can reach through experimental calculation3, illuminating area productivity ratio is up to 10.7gm-2·d-1(dry), the energy consumption about 560kJ/g of the nostoc of unit mass.
The stacked nostoc Thin cell layer device of embodiment 1
Such as Figure 1It is shown, the culture plate 1 of the culture apparatus including multiple stacked distributions in this example, culture plate 1 (Figure 2) including base plate 11 and around base plate 11 and the closely coupled cofferdam 12 of base plate 11, cofferdam 12 is higher than the about 1-20mm of base plate 11, and base plate 11 forms the culture space of nostoc with cofferdam 12, a preset distance is spaced between neighbouring two culture plate 1.
There is support member 2 between neighbouring two culture plate 1, support member 2 makes to form described preset distance between neighbouring culture plate 1.Support member 2 is support column in this example.Upper and lower two adjacent culture plate 1 is supported by multiple support columns, and the base plate 11 of the top of each support column with bottom respectively with two culture plates 1 connects.
The shape of support column, material, quantity, spacing and arrangement can not be limited, and only need support column to have some strength, and the nutrient solution in the culture plate of stacked on top and culture plate is supported on enough.Support column uses transparent material, such as glass, acrylic, PC materials.Support column can have any suitable shape, including but not limited to cylinder, tubular, square, strip etc..
Such as Figure 3It is shown, there is light source 3 in the side of the culture apparatus, different from the upside or top that general light source is arranged at culture systems, the light source 3 in this example is provided in the side of culture apparatus, vertical with culture plate 1.
Because the present invention uses Thin cell layer, therefore the weight that culture plate is born is relatively low, therefore can be more frivolous, such that it is able to improve the transparency of culture plate, is conducive to passing through for light.Also, due to there is gap between each layer culture plate, cofferdam is also transparent material, therefore light can laterally approach into above culture plate and bottom surface so that the nostoc light wherein cultivated is more fully and uniform, substantially increases the utilization ratio of light source.
The nostoc thin layer quiescent culture of embodiment 2 and the comparing with traditional training method
In order to show the Advantageous Effects of cultural method of the invention and culture apparatus, investigated with " unit area light source yield " " power consumption of unit mass algae powder " " cost " " flat light source area water consumption " four factors directly related with industrialization benefit below, and to be verified.
Experimental group：
Such as Figure 4AShown, using Thin cell layer device and training method described in embodiment 1, left side is that length is 1m, is highly the light source 3 of 1m, 274.6 μm of olm of light intensity-2·s-1.Right side is culture plate array, and culture plate is glass material, and specification is 120mm*60mm, and thickness of glass is 1.5mm.The height in cofferdam is 2.5mm (nutrient solution is thick).The open ended algae solution amount of each culture plate is 18ml (120mm*60mm*2.5mm).Support column height is 10mm.1m is laminated in vertical direction.Environment temperature controls 25 degree or so, relative humidity 80%-99%.Nutrient solution is that the BG11 fluid nutrient medium initial inoculations dry weight without nitrogen is 0.3g/L.Whole environment is tested in the case of a completely obscured natural light, excludes the interference of natural light.
At the beginning of culture, detected through light-intensity test instrument, drain into the 7th row near lamp source the 1st, the light intensity of media surface isFigure 5.FromFigure 5Local light intensity as can be seen that due to having support column between the flat board that is laminated, between layers in the presence of a large amount of tiny spacing, light can always be turned right by the gap of interlayer and is continuously passed on and more remote is weaker.(Under FigureIt is successively from left to right the 7th row, the light intensity value of the row's media surface of the 6th row ... the 1st)
And it is observed that the sheet glass of the 1st row is because close to light source, preferably, poorer further away from light source lamp plate upgrowth situation, the growing state of the 7th and 6 rows is worst for upgrowth situation.Treat that culture is harvested for 11 days afterwards, during results, carry out statistics algae biology total amount, growing state respectively according to different rowsAs schemed 5ColumnFigure.Can therefrom see, although the algae dry weight of the 6th and 7 rows is smaller, because light can be reached at this, therefore nostoc at this can still obtain light source and grow.(On FigureIt is successively from left to right the 7th row, the biomass (ordinate) of the row of the 6th row ... the 1st collection)
The algae that all flat layers in above-mentioned model are harvested is total together, and total amount is 145.2g.Nutrient solution cumulative volume V=(1000/100) × 7 × 18ml × (1000/11.5)=90.3L, then it is 1.608g/L to calculate the average algae dry weight density for harvesting.
Control group 1：
Using in goldfish jar culturing room culture (Figure 4C) indoor culture.Condition of culture is as follows：Culture light intensity is 120-250 μm of olm-2·s-1, cultivation temperature is 25 DEG C, and gas is passed through with air pump, and throughput is 0.1-1L/min, and inoculum density is 0.1g/L (dry weight).
Control group 2：
Using goldfish jar (Figure 4C) cultivated in outdoor.Condition of culture is as follows：Cultivation temperature is outdoor environment temperature, and culture light intensity is natural light, and gas is passed through with air pump, and throughput is 0.1-1L/min, and inoculum density is 0.02g/L (dry weight).
Control group 3：
Using open raceway pond outside scenery (Figure 4B).Condition of culture is as follows：Cultivation temperature is outdoor environment temperature, and culture light intensity is natural light, and the mixer electric stirring of 0.75KW, inoculum density is 0.05-0.13g/L (dry weight).
For raceway pond, its culture liquid level is higher, and light is decayed in water and is exceedingly fast, so cause light to be difficult to be penetrated into the bottom of raceway pond, and goes to pond and to continue on mixing pump agitation cycle.
Energy consumption is similarly needed to drive agitating device for glass jar, while 1 ㎡ intensity is 274.6 μm of olm-2·s-1The light sent on the right side of light source is only capable of the growth for nostoc in a goldfish jar【In the case of natural light is not considered】, then the goldfish jar of arrangement of turning right cannot obtain efficient light sources, therefore utilization rate of both reactors to luminous energy is relatively low.I.e. whole environment is tested in the case of a completely obscured natural light, excludes natural light interference.The light source of 1 ㎡ irradiates a side of traditional plate container type reactor, plate container side is 1 ㎡, width is 20cm, light acutely weakens (determine optical energy loss rate and reach more than 95%) through the light intensity after 20cm algae solutions, luminous intensity for algae normal growth, cannot cause the light source of 1 ㎡ to be only capable of direct irradiation to come nearest first row goldfish jar culture vessel.(Such as Figure 4C)。
Result of the test and conclusion：
According to above-mentioned experimental group and the model of control group, it (is 274.6 μm of olm with 1 ㎡ intensity that thin layer of the invention, high density are laminated culture apparatus and are compared as follows with traditional plate container-2·s-1Light source is references object)：
Thin cell layer method of the present invention, at aspects such as unit area light source yield, energy consumption cost and water consumption, is significantly better than traditional training method as known from the above.
Further, since cultural method of the present invention and device are to cultivate in a static manner, period need not supply high pressure CO2Realize that air-flotation type is stirred, the energy consumption equipment such as homogenized without circulating pump, agitating paddle etc., and the algae that the inventive method is harvested be in half wet condition, reduces the energy consumption of concentration after harvesting.
Additionally, being 0.1g/L, 0.3g/L, 0.6g/L in inoculum density according to the training method and condition of experimental group, unit area light source yield of the present invention is respectively 6.8gm during 0.9g/L-2·d-1, 10.7gm-2·d-1, 8.05gm-2·d-1、8.3g·m-2·d-1.By result understand inoculum density 0.3g/L when unit area light source yield highest, but the still more traditional plate container of the yield of the unit area of other inoculum densities is much higher.Thus the result that obtains at present is inoculum density when being 0.1-0.9g/L, and the utilization ratio of energy consumption is higher.
In addition, if according to the training method and condition of experimental group, inoculum density 0.3g/L, when nutrient solution thickness is respectively 1mm, 1.5mm, 2mm, 3mm, 5mm, 7mm, 10mm, 15mm, 20mm, the unit area light source yield of the present invention when interlamellar spacing is 25mm is respectively 4.47gm-2·d-1、4.86g·m-2·d-1、5.21g·m-2·d-1、7.79g·m-2·d-1、9.60g·m-2·d-1、8.33g·m-2·d-1、5.85g·m-2·d-1、5.09g·m-2·d-1、3.87g·m-2·d-1；When wherein the thickness of nutrient solution is 2mm to 10mm, nostoc is higher to the utilization ratio of light.
Claims (23)
1. a kind of microalgae culture method, its be by microalgae in Thin cell layer liquid quiescent culture, enable the microalgae directly using the CO of Thin cell layer liquid Surface absorption2。
2.According to claimCultural method described in 1, it is characterised in that the thickness of the nutrient solution is 1mm~20mm.
3. a kind of cultural method of nostoc, its be by nostoc in Thin cell layer liquid quiescent culture, enable the nostoc directly using the CO of Thin cell layer liquid Surface absorption2。
4.According to claimCultural method described in 3, it is characterised in that the thickness of the nutrient solution is 1~4 times of the fresh grain diameter range of cultivated nostoc.
5.According to claimCultural method described in 3 or 4, it is characterised in that the thickness of the nutrient solution is 1mm~20mm.
6.According to claimCultural method described in 5, it is characterised in that the thickness of the nutrient solution is 2mm~10mm.
7.According to claimCultural method described in any one of 3-6, it is characterised in that the environment temperature of the quiescent culture is 20-28 DEG C.
8.According to claimCultural method described in any one of 3-6, it is characterised in that the luminous intensity of the quiescent culture is 2~526 μm of olm-2·s-1, more preferably 16.9~274.6 μm olm-2·s-1。
9.According to claimCultural method described in any one of 3-6, it is characterised in that the culture medium of the quiescent culture is the BG11 culture mediums without nitrogen.
10.According to claimCultural method described in any one of 3-6, it is characterised in that the ambient humidity range of the quiescent culture is 80-99%.
11.According to claimCultural method described in any one of 3-6, it is characterised in that nostoc inoculum concentration is 0.1-0.9g/L.
A kind of 12. stacked nostoc Thin cell layer devices, its culture plate for including multiple stacked distributions, the culture plate includes base plate and around base plate and the closely coupled cofferdam of base plate, the lowest part in the cofferdam is highly 1mm-20mm, and a preset distance is spaced between neighbouring two culture plate.
13.According to claimThin cell layer device described in 12, it is characterised in that have support member between neighbouring two culture plate, support member makes to form described preset distance between neighbouring culture plate.
14.According to claimThin cell layer device described in 13, it is characterised in that base plate of the support member respectively with neighbouring two culture plate is connected.
15.According to claimThin cell layer device described in 13 or 14, it is characterised in that the support member is support column.
16.According to claimThin cell layer device described in any one of 13-14, it is characterised in that the spacing between neighbouring two culture plates base plate and base plate is 5~30mm.
17.According to claimThin cell layer device described in any one of 13-14, it is characterised in that the base plate of the culture plate and/or the material in cofferdam are transparent material.
18.According to claimThin cell layer device described in 17, it is characterised in that the base plate of the culture plate and/or the material in cofferdam be selected from：Glass, GPPS, ABS, styrene-acrylonitrile, PVC, PMMA, polycarbonate or polystyrene.
19.According to claimThin cell layer device described in 13 or 14, it is characterised in that the material of the support member is transparent or light transmissive material.
20.According to claimThin cell layer device described in any one of 12-14, it is characterised in that the culture plate is formed in one.
21.According to claimThin cell layer device described in 13 or 14, it is characterised in that the culture plate is formed in one with the support member.
22.According to claimThin cell layer device described in any one of 12-14, it is characterised in that the baseboard material of the culture plate is plate glass, the bank material of the culture plate isGelatinBar.
23.According to claimThin cell layer device described in any one of 12-14, it is characterised in that also have light source at least one side of the Thin cell layer device, the light source is vertical with culture plate.
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| CN108148787A (en) * | 2018-02-07 | 2018-06-12 | 青岛浩然生态海洋水产科技有限公司 | A kind of method of pilot scale culture nostoc |
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