CN106900536A - Photo-thermo sensitive male nuclear sterile rice line orients transformation into the method for protection cytoplasmic male sterile line - Google Patents
Photo-thermo sensitive male nuclear sterile rice line orients transformation into the method for protection cytoplasmic male sterile line Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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Abstract
Transformation into the method for protection cytoplasmic male sterile line is oriented this application provides photo-thermo sensitive male nuclear sterile rice line, it includes：Maternal by backcrossing of photo-thermo sensitive male nuclear sterile rice line, rice-cultivating is male parent, and by hybridization, backcrossing and selfing, seed selection obtains the homotype fertile line of photo-thermo sensitive male nuclear sterile rice line；It is again backcrossing male parent with the homotype fertile line of the photo-thermo sensitive male nuclear sterile rice line, rice protection cytoplasmic male sterile line is female parent, by hybridizing and being returned, seed selection obtains the rice new strain that genetic property changes, the i.e. new protection cytoplasmic male sterile line being bred as, its excellent genetic background at utmost retaining former photo-thermo-sensitive genic male sterile line, Comprehensive Traits and Breeding Potential, pollen sterility degree is high, sterility stabilization, do not disturbed by nature illumination length and temperature height change, run into weather conditions complicated and changeable, still hybrid paddy rice production of hybrid seeds seed high-purity is ensured.
Description
Technical field
The application belongs to technique for breeding of hybridized rice field, and in particular to rice photo-thermo-sensitive Genetic Sterility
Method of system's orientation transformation into protection (CMS-FA) cytoplasmic male sterile line.
Background technology
Stone bright pine in Hubei is found that male sterile plant in the colony of japonica rice land reclamation and cultivation 58 within 1973,
In summer, heading shows as male sterility to the plant, and autumn heading then shows as male-fertile, warp
Study its pollen fertility has close ties with temperature height and illumination length, i.e., long in summer high temperature
Ear under sunshine weather conditions and show as male sterility, and low temperature short-day weather conditions in the fall
Lower heading then shows as male-fertile, and the sterile line is named as into Hubei photoperiod-temperature sensitive genie male-sterile line water
Rice.
Since proposing the concept of paddy rice nature dual purpose lines from Shi Mingsong (1981), many has been selected
Dual-purpose photo-thermo sensitive male nuclear sterile rice line, and pushed away by authorization with composition two-line hybrid rice combination
Extensively, the new way of rice heterosis utilization is opened, and is developing progressively as hybrid rice educates
A kind of important kind of the technology of kind, is China's hybrid paddy rice technological innovation, continues to keep advanced in the world
Level is made that tremendous contribution.
The breeding research of photo-thermo sensitive male nuclear sterile rice line is the most crucial technology in the field, relevant to grind
Study carefully and discuss that the representative document of photo-thermo sensitive male nuclear sterile rice line breeding technique has：
(1)《Super hybridized rice is studied》, Yuan Longping chief editor, 2006, Shanghai science and technology went out
Version society, Shanghai.
(2)《Hybrid rice is learned》, Yuan Longping chief editor, Chinese agriculture publishing house, north in 2002
Capital.
(3)《The theory and technology of double-hybrid rice strains》, Chen Liyun chief editors, 2001, on
Extra large science tech publishing house, Shanghai.
(4)《Male sterility of rice biology》, Zhu Yingguo volumes, 2000, Wuhan University published
Society, Wuhan.
(5)《Hybrid vigour and hybrid rice breeding》Cui Yang Ren, Chen Shunhui is translated, 1996, Fujian section
Technology publishing house, Foochow (Heterosis and Hybrid Rice Breeding S.S.Virmani
Write, Springer-Verlay Berlin Heidelberg publishing houses, 1994, Germany).
(6)《Rice breeding》, Min Shaokai, Shen Zongtan, bear shake the people volume, 1996, China
Agriculture publishing house, Beijing.
(7)《Photaesthesia line with genic sterile rice fertility switching mechanism and application study》, Li Ze Ping volumes,
Nineteen ninety-five, Hubei science tech publishing house, Wuhan.
(8)《The research of double-linear hybrid rice and application》, He Haohua, unit is given birth to towards volume, 1993,
In Jiangxi Science technology publishing house, Nanchang.
(9)《Photoperiod-sensitive nuclear sterile rice is studied and utilized》, Zhu Yingguo, poplar generation normal volume,
, publishing house of Wuhan University, Wuhan in 1992.
(10)《Rice in China kind and its pedigree》, Lin Shicheng, Min Shaokai volume, 1991, on
Extra large science tech publishing house, Shanghai.
(11) " discovery of the recessive male sterile rice sensitive to illumination length and Primary Study ",
Shi Mingsong, 1985,《Scientia Agricultura Sinica》(2):44-48.
(12)《The research of hybrid rice and practice》, Li Ze Ping etc. compiles, nineteen eighty-two, Shanghai science
Technology publishing house, Shanghai.
1st, rice photo-thermo-sensitive sterile line and two-line hybrid rice breeding technique advantage and achievement
Had in terms of breeding technique using photo-thermo-sensitive genetic male sterile line approach seed selection two-line hybrid rice and permitted
Many advantages：One is that photo-thermo-sensitive genetic male sterile line breeding does not need maintainer, as long as arranging to be planted in rice work
Region autumn or Hainan spring ear, and temperature and illumination can just meet that induction pollen turns into can
The condition educated, realizes the purpose of self propagated sterile line, is reduced between maintainer in production
Every the link (such as Yebai three line method sterile line) of the breeding male sterile lines of plantation pollination, can reduce
Production cost, increases economic efficiency；Two is photo-thermo-sensitive genetic male sterile line combo freedom, has studied table
Bright 98% common rice cultivar can recover the pollen fertility of Dual purpose male-sterile line, apolegamy
The probability of superior hybrid crosses paddy rice is increased substantially.And Yebai cytoplasmic male sterile line only has
20% or so kind can be as restorer, by contrast, the restorer of two-line hybrid rice
Utilization rate is much higher than the restorer utilization rate of Yebai cytoplasmic hybrid paddy rice；Three is current science
Boundary generally believes that the temperature sensitive sterility of light controls heredity by karyogene, unrelated with cytoplasm, thus may be used
Overcome cytoplasmic negative effect and cytoplasm in Yebai three line method sterile line it is single bring it is potential
It is dangerous；Four can be followed and using the achievement of conventional Rice breeding advance, except can rapidly improve rice
Outside matter, moreover it is possible to subspecies indica and japonica hybrid advantage is utilized, to realize hybrid rice higher yield water
Flat breeding objective.
From the research temperature sensitive sterile gene of Nongken58s light, carry out dual purpose genic male sterile line and two line method is miscellaneous
Since handing over rice breeding to study, so far, national paddy rice data center
(http://www.ricedata.cn/) list the photo-thermo-sensitive genetic male sterile line information for obtaining identification above the provincial level
More than 70 (bars), surpassed by identification above the provincial level and the sterile line for obtaining kind power mandate
Cross 19 (bars).National paddy rice data center also shows that cut-off 2013 has obtained provincial
The double-linear hybrid rice combination of authorization above is up to 606 (bar).Counted according to 2008, China
Two-line hybrid rice cultivated area accounts for 25% or so of the total sown area of hybrid paddy rice, Anhui, Hunan,
The two-line hybrid rice cultivated area in the provinces such as Hubei alreadys exceed Three-line Hybrid rice.1993-2009
Add up popularizing area between year and reach 3.15 hundred million mu.Two-line hybrid rice has become China's Rice Production
An important type in, played an important role in Ensuring Food Safety and good harvest.
Double-hybrid rice strains breeding technique be also generalized to India, the U.S., Vietnam, Philippine,
Japan and other countries, gradually go to the world.
2nd, the production risk of rice photo-thermo-sensitive sterile line and two-line hybrid rice
Rice photo-thermo-sensitive sterile line is promoted with two-line hybrid rice breeding and achieves the same of great achievement
When, potential risks cannot be avoided, and be become increasingly conspicuous.
So far, the rice photo-thermo-sensitive sterile line of scientist's design follows the Nature illumination and temperature
The change of degree regulates and controls pollen fertility, for example, inducing under summer day hot conditions long light temperature sensitive not
The pollen sterility for being is educated, the hybrid paddy rice production of hybrid seeds is arranged；Induce light temperature sensitive under autumn short day cryogenic conditions
The pollen of sterile line can educate, and arrange breeding male sterile lines.
But, because weather is complicated and changeable under the conditions of the Nature, it is impossible to predict and uncontrollable
Summer Low Temperature, cold wave usually induce the pollen of seed farm part rice photo-thermo-sensitive sterile line to be converted to
Can educate or half can educate, these can be educated with half fertile pollen by self-pollination, still produce light temperature
Quick sterile line, is mingled in the hybrid rice seed of seed farm, causes seed purity of hybrid rice significantly
Degree declines, it is impossible to reaches commodity seed standard and brings about great losses.
Due to uncertain climate change cause the production of hybrid seeds failure or seed purity not phenomenon up to standard when
There is generation.In addition to by outside climate change effect fertility, the heredity drift of sterile line itself
Become phenomenon and can result in fertility instability, because sterile line genetic background is subject to controlled by multiple genes,
With the increase of algebraically, its Fertility Transfer Temperature can drift about upwards, and fertility produces fluctuation.
In sum, rice photo-thermo-sensitive sterile line Breeding Application obtain great achievement while,
Lie dormant huge production risk.So far, the scientific and technological level of the mankind still cannot have
Effect prediction Summer Low Temperature, cold wave.Once Summer Low Temperature, cold wave arrive, then, the mankind are existing
Production technology level and ability resist and control Summer Low Temperature, cold wave it is extremely limited.
Therefore, for a long time, it is difficult to dissolve the summer from existing Research Thinking and production application aspect
Season low temperature, sterile line pollen fertility this problem for attacking in two-line hybrid rice seed farm of cold wave,
Potential risks threaten two-line hybrid rice seed to produce all the time.
The content of the invention
On the one hand, it is thin into protection the invention provides photo-thermo sensitive male nuclear sterile rice line orientation transformation
The method of cytoplasm male sterility line.
The photo-thermo sensitive male nuclear sterile rice line that the present invention is provided orients transformation into protection cytoplasmic male
The method of sterile line is comprised the following steps：It is maternal by backcrossing of photo-thermo sensitive male nuclear sterile rice line, plant
Training paddy rice is male parent, and by hybridization, backcrossing and selfing, seed selection obtains rice photo-thermo-sensitive Genetic Sterility
The homotype fertile line of system.
On the other hand, the photo-thermo sensitive male nuclear sterile rice line that the present invention is provided orients transformation into protection
The method of cytoplasmic male sterile line is comprised the following steps：With photo-thermo sensitive male nuclear sterile rice line as return
Hand over maternal, rice-cultivating is male parent, by hybridization, backcrossing and selfing, seed selection obtains paddy rice light
The homotype fertile line of temperature-sensitive sterile line；And with the homotype of the photo-thermo sensitive male nuclear sterile rice line
Fertile line is backcrossing male parent, and rice protection cytoplasmic male sterile line is female parent, by hybridization
And backcrossing, the rice new strain that seed selection acquisition genetic property changes, i.e., newly the protection of incubation is thin
Cytoplasm male sterility line.
In one embodiment, the genetic property change include the sterility of paddy rice to illumination with
Temperature-insensitive.
In another embodiment, the homotype of the seed selection acquisition photo-thermo sensitive male nuclear sterile rice line can
Educating the step of being includes 2-8 backcrossing and 3-5 selfing, preferably 4-7 times backcrossing and 4 selfings,
More preferably 6 times backcrossings and 4 selfings.
In yet another embodiment, wherein the seed selection obtains the paddy rice new product that genetic property changes
The step of being includes 2-8 backcrossing, preferably 4-7 times backcrossing, more preferably 6 times backcrossings.
In yet another embodiment, the photo-thermo sensitive male nuclear sterile rice line is：Light high temperature-induced sterility long
Type line with genic sterile, high temperature-induced sterility type line with genic sterile, short smooth low temperature infertility type line with genic sterile or low
Warm infertility type line with genic sterile.In optional embodiment, the photo-thermo sensitive male nuclear sterile rice line
Extensively to account for 63-4s, numerous 8s or double 8s etc.；And/or the rice protection cytoplasmic male sterility
It is new source 5A, peasants who dig gold 1A, peasants who dig gold 2A or peasants who dig gold 3A etc. to be.
In one embodiment, the photo-thermo sensitive male nuclear sterile rice line orientation that the present invention is provided turns
The method for being bred as protection cytoplasmic male sterile line is comprised the following steps：1) with rice photo-thermo-sensitive
Sterile line extensively account for 63-4s for female parent, using its with carried out as the rice-cultivating Zhejiang 987 of male parent it is miscellaneous
Hand over, obtain cenospecies；2) 63-4s as backcrossing female parent is extensively accounted for rice photo-thermo-sensitive sterile line, will
The step of it is with as male parent 1) cenospecies that is obtained is returned, continues to be returned about 6 times,
Obtain backcrossing kind；And 3) by step 2) the backcrossing kind that is obtained carries out 3-5 selfing, seed selection
The homotype fertile line for obtaining photo-thermo sensitive male nuclear sterile rice line extensively accounts for 63-4B.
In another embodiment, the photo-thermo sensitive male nuclear sterile rice line orientation that the present invention is provided
Transformation is comprised the following steps into the method for protection cytoplasmic male sterile line：1) with paddy rice light temperature
It is female parent that quick sterile line extensively accounts for 63-4s, and it is carried out with the rice-cultivating Zhejiang 987 as male parent
Hybridization, obtains cenospecies；2) 63-4s as backcrossing female parent is extensively accounted for rice photo-thermo-sensitive sterile line,
Using its with as the step of male parent 1) cenospecies that is obtained is returned, continues to be returned about 6 times,
Obtain backcrossing kind；And 3) by step 2) the backcrossing kind that is obtained carries out 3-5 selfing, seed selection
The homotype fertile line for obtaining photo-thermo sensitive male nuclear sterile rice line extensively accounts for 63-4B；4) with homotype fertile line
The wide 63-4B that accounts for for male parent, using it and as maternal rice protection cytoplasmic male sterile line
New source 5A is hybridized, and obtains cenospecies；And 63-4B extensively 5) is accounted for homotype fertile line be
Backcrossing male parent, using it and as maternal step 4) cenospecies that is obtained is returned, continued
Backcrossing 6 times, seed selection obtains the rice new strain that genetic property changes, i.e., the new protection being bred as
Cytoplasmic male sterile line extensively accounts for 63-4A.
In the above-described embodiment, the photo-thermo sensitive male nuclear sterile rice line, the rice photo-thermo-sensitive
The rice new strain that the homotype fertile line of line with genic sterile or the genetic property change be selected from paddy rice,
Upland rice, long-grained nonglutinous rice, japonica rice, early rice, late rice, non-glutinous rice, glutinous rice.Optionally, the paddy rice light
Temperature-sensitive sterile line, the homotype fertile line of the photo-thermo sensitive male nuclear sterile rice line or the heredity
The rice new strain that attribute changes is selected from rice leaf hairiness rice and rice leaf without hair rice.
It can be seen that, the photo-thermo sensitive male nuclear sterile rice line that the application is provided orients transformation into protection cell
The method of matter male sterile line, at least possesses one of following advantage：Retain rice photo-thermo-sensitive not
Educate assemblage conjunction genetic background constant, change the heredity of male sterility attribute of the sterile line, cultivated
Into illumination length and temperature is not received, just change is disturbed, pollen sterility degree is high, and/or sterility is steady
Fixed new lines, i.e., new protection (CMS-FA) cytoplasmic male sterile line being bred as or new germplasm source
(CMS-FA) sterile line.Therefore, in hybrid paddy rice production of hybrid seeds production, using this new sterile line
Method, even if running into low temperature complicated and changeable, cold wave weather conditions, still can produce guarantor
Height male sterility, and/or high-purity hybrid rice seed are held, high-purity seed quality mark is reached
It is accurate.
Specific embodiment
As used herein, " paddy rice " is any rice plant and including can be all with rice breeding
Plant variety.As used herein, " plant " or " plant ", including whole plant, plant cell,
Plant cell tissue cultures that plant organ, plant protoplast, plant can therefrom regenerate,
Complete plant cell in plant callus, vegetation bed and plant or plant part, the plant
Part for example embryo, pollen, ovule, seed, leaf, flower, branch, fruit, cane, root, the tip of a root,
Flower pesticide etc..Paddy rice as used herein, including paddy rice, upland rice, long-grained nonglutinous rice, japonica rice, early rice, evening
Rice, non-glutinous rice, glutinous rice, rice leaf hairiness rice and rice leaf are without hair (naked body rice) rice.
As used herein, " photo-thermo sensitive male nuclear sterile rice line ", including light high temperature-induced sterility type long, high temperature
Sterile type, short smooth low temperature infertility type, low temperature infertility type line with genic sterile etc., for example extensively account for 63-4s,
Numerous 8s or double 8s etc..
As in the symbol that this paper breeding methods are used, " F " represents hybridization；" B " represents backcrossing；
" n " represents backcross generations number.
As used herein, " height male sterility " refers to 1% iodo- iodine of Matured Pollen In Rece
Change potassium (I2- KI) solution dyeing after, put 100 × optical microphotograph Microscopic observation POLLEN MORPHOLOGY and dyeing journey
The sample that degree, wherein pollen sterile reach more than 99.9% is referred to as height male sterility.
As used herein, " high-purity hybrid rice seed " refers to qualified hybrid paddy rice commodity seed standard
Purity must reach more than 96%.
As used herein, " sterile line " is also called " male sterile line " or " cytoplasmatic male is not
Educate and be ", i.e., pollen sterility and this feature can be entailed the rice strain of offspring.
As used herein, " the homotype fertile line of photo-thermo sensitive male nuclear sterile rice line ", refer to and paddy rice light
Temp-sensing sterile line complex inheritance background is similar, economical character is similar, Breeding Potential is similar, but
Pollen and small ear selfing fertile new lines completely, because the strain can be transferred to newly by backcrossing
Into further cultivating into new germplasm source (CMS-FA) sterile line after matter source cell matter, thus it is referred to alternatively as
The homotype maintainer of new germplasm source or B systems, what is be just bred as is referred to as new B systems.
As used herein, " new germplasm source (CMS-FA) sterile line ", " new germplasm source (CMS-FA) male
Sterile line ", " protection (CMS-FA) cytoplasmic male sterile line ", " protection (CMS-FA)
Male sterile line ", or " protection (CMS-FA) sterile line " is alternatively used, and is referred both to from good fortune
Wild rice (O.rufipogon) is built to research and develop successfully and with Wild in Fujian Province rice male sterile cytoplasm
Male sterile line, i.e., with the sterile line of specific CMS-FA cytoplasm background, abbreviation new germplasm source
CMS-FA male sterile lines or CMS lines with new cytoplasm.Because of the cytoplasmic maintainer utilization of resources
55.5% is reached, than Yebai (CMS-WA) the maintainer utilization of resources 20% more extensively, thus
Be otherwise known as protection cytoplasmic male sterile line, and protection male sterile line or protection are sterile
System, for example, referring to Chinese invention patent document CN1954666B.
Photo-thermo sensitive male nuclear sterile rice line is oriented transformation into protection by the method provided using the application
(CMS-FA) cytoplasmic male sterile line, its proterties is neat, stabilization, complex inheritance background, comprehensive
Close economical character similar to the homotype fertile line of photo-thermo sensitive male nuclear sterile rice line, but pollen and small
Fringe is completely sterile, also referred to as new A systems, or new new germplasm source (CMS-FA) male sterile line being bred as.
The orientation transfer method of the photo-thermo sensitive male nuclear sterile rice line that the application is provided, it then follows following
Two basic principles：One is the excellent heredity back of the body for not changing rice photo-thermo-sensitive sterile line germplasm substantially
Scape, comprehensive agronomy proterties and Breeding Potential, the excellent rice photo-thermo-sensitive that long-term breeding is accumulated is not
Educate is that germ plasm resource continues to be played a great role in hybrid rice breeding；Two is using innovation heredity
Thremmatology method, changes the heredity of male sterility attribute of sterile line, rice photo-thermo-sensitive sterile line pair
The sensitive characteristic changing of environmental change such as illumination length and temperature height turns into gentle to illumination length
Insensitive protection new germplasm source (CMS-FA) nucleo-cytoplasmic interreaction heredity control of temperature low environmental change high
The male sterility characteristic of system, pollen sterility is settled out.
In this way, in hybrid paddy rice production of hybrid seeds production application, once run into Summer Low Temperature, cold wave attack
When, the pollen fertility of sterile line remains in that height male sterility, does not produce pollen fertility to change,
High-purity hybrid rice seed is produced in guarantee, and this is fundamentally dissolved by technological innovation means
Long unresolved, far-reaching science and production problem.
It can be seen that, the application utilizes the technical advantage of new germplasm source (CMS-FA) hybrid paddy rice technology platform,
Reservation rice photo-thermo-sensitive sterile line complex inheritance background is constant, and the sterility for changing the sterile line is lost
Attribute is passed, is cultivated into not by illumination length and temperature height change interference, pollen sterility degree
High, sterility stabilization new germplasm source (CMS-FA) sterile line.This new sterile line is applied miscellaneous
Hand in rice production of hybrid seeds production, even if running into low temperature complicated and changeable, cold wave weather conditions, still may be used
To keep height male sterility, high-purity hybrid rice seed is produced, reach high-purity seed
Quality standard, fundamentally solves China's rice photo-thermo-sensitive sterile lines seed making security and purity not
Problem high.
Specifically, in one embodiment, this application provides photo-thermo sensitive male nuclear sterile rice line
Into the breeding method of protection (CMS-FA) sterile line, agent technology area is two steps for orientation transformation
Suddenly：
The first step, by photo-thermo sensitive male nuclear sterile rice line transformation into rice photo-thermo-sensitive sterile line synthesis
Genetic background is similar, economical character is similar, Breeding Potential is similar, but pollen and small ear selfing
Completely normal fertile line, or be B systems.
Female parent is done with rice photo-thermo-sensitive sterile line, conventional rice kind does male parent, phase mutual cross,
Obtain hybrid seed.Hybrid seed is planted into hybridization F1 plant, after heading, with paddy rice light temperature
Quick sterile line does female parent, and male parent backcrossing is done with F1, obtains backcrossing seed B1F0.By backcrossing kind
Son plants into B1F1 plant, and after heading, continuation rice photo-thermo-sensitive sterile line does female parent, with
B1F1 does male parent backcrossing, and such as the method continues to be returned 2-7 times, obtains BnF0 backcrossing seeds.
Plantation BnF1 seeds, heading relief its selfing, harvesting wherein pollen and small ear can educate plant completely
The selfed seed 2000 or so of strain.1000 plants or so of BnF2 plant are planted into, after heading
Allow its selfing, pollen and the complete normal plant of Spikelet fertility are selected after maturation, point individual plant is harvested to be protected
Deposit 50 plants of selfed seeds.Plant into BnF3 50 strains from generation to generation, each strain plantation 100
Strain.Heading relief its selfing, selects the pollen and small ear of whole plant in cell complete after maturation
Can normally educate, be separated without sterile plant, complex inheritance background is similar to rice photo-thermo-sensitive sterile line,
Comprehensive agronomy proterties is similar to rice photo-thermo-sensitive sterile line, and as rice photo-thermo-sensitive sterile line is same
The normal fertile line of type.Due to the strain can further transformation into new germplasm source (CMS-FA) infertility
System, thus the homotype maintainer of new germplasm source is referred to alternatively as, or be B systems or new B systems.
Second step, female parent, the new B that the above method is bred as are done by new germplasm source (CMS-FA) sterile line
Male parent is done by system, and phase mutual cross obtains hybrid seed.F1 plant are planted into, F1 is used after heading
Female parent is done, male parent backcrossing is done by new B systems, obtains backcrossing seed.B1F1 plant are planted into, is taken out
Female parent is done with B1F1 plant after fringe, male parent is done by new B systems, continue to be returned.As the method is continuous
Backcrossing 2-7 times, obtains BnF1 plant, referred to as new A systems.The tree characteristics of new A systems neatly,
Stabilization, complex inheritance background is similar to new B systems, comprehensive agronomy proterties is similar to new B systems, but
It is that pollen and small ear are completely sterile, new A systems are new new germplasm source (CMS-FA) male being bred as not
Educate and be, that is, photo-thermo sensitive male nuclear sterile rice line orient transformation into protection (CMS-FA) infertility
System.
Protection (CMS-FA) sterile line used herein, refers to Wild in Fujian Province rice
(O.rufipogon) Cytoplasmic male sterility source male sterile line, abbreviation CMS lines with new cytoplasm,
Or sterile line.The sterile line Stamen development is normal, and the development degeneration of stamen or abortion, it is impossible to
Self-pollination is solid.
New germplasm source (CMS-FA) infertility maintainer used herein, refers to Wild in Fujian Province rice
(O.rufipogon) novel fine kytoplasm source male sterility maintainer line, abbreviation maintainer.The maintainer
Pistil And Stamen development is normal, and its pollen is authorized the gynoecium of male sterile line, solid can not only obtain
Ripe health seed, and the plant for after planting growing up to is still male sterile plant.
New germplasm source (CMS-FA) sterile restoring system used herein, refers to Wild in Fujian Province rice
(O.rufipogon) source of cytoplasm male sterile restoring line, abbreviation restorer.By restorer flower
Powder authorizes the gynoecium of sterile line, and after planting, the plant for growing up to has recovered hero to produced seed again
Property (pollen) can normally educate.
Above-mentioned photo-thermo sensitive male nuclear sterile rice line orients transformation into protection (CMS-FA) sterile line
Using in symbol, F represents hybridization to breeding method；B represents backcrossing；N represents backcross generations number.
Above-mentioned photo-thermo sensitive male nuclear sterile rice line orients transformation into protection (CMS-FA) sterile line
The signified paddy rice of breeding method, including paddy rice, upland rice, long-grained nonglutinous rice, japonica rice, early rice, late rice,
Non-glutinous rice, glutinous rice, rice leaf hairiness rice and rice leaf are without hair (naked body rice) rice.
Above-mentioned photo-thermo sensitive male nuclear sterile rice line orients transformation into protection (CMS-FA) sterile line
The signified photo-thermo sensitive male nuclear sterile rice line of breeding method, including light high temperature-induced sterility type line with genic sterile long,
High temperature-induced sterility type line with genic sterile, short smooth low temperature infertility type line with genic sterile, low temperature infertility type Genetic Sterility
System.
Above-mentioned photo-thermo sensitive male nuclear sterile rice line orients transformation into protection (CMS-FA) sterile line
The signified photo-thermo-sensitive genic male sterile line of breeding method, including paddy rice, upland rice, long-grained nonglutinous rice, japonica rice, morning
Rice, late rice, non-glutinous rice, glutinous rice, rice leaf hairiness rice and rice leaf are temperature sensitive without hair (naked body rice) rice type light
Line with genic sterile.
Therefore, the photo-thermo sensitive male nuclear sterile rice line that the application is provided orients transformation into protection
(CMS-FA) breeding method of sterile line, obtains new product, i.e., the new new germplasm source being bred as
(CMS-FA) the technical characterstic outstanding behaviours of sterile line (referred to as new A systems, same as below) is at least
One of following aspects：
(1) the new CMS lines with new cytoplasm being bred as is than more fully retaining former photo-thermo-sensitive genic male sterile line
Good genetic background, comprehensive agronomy proterties and comprehensive breeding potentiality：New A systems use paddy rice light temperature
Quick line with genic sterile hands over transformation to form as recurrent parent by 2-7 times.Thus, new A systems ratio
More fully remain complex inheritance background, comprehensive agronomy that former photo-thermo-sensitive genic male sterile line possesses
Shape and comprehensive breeding potentiality.
(2) pollen sterility of new A systems is by nucleus and cytoplasm interaction co- controlling：
New A systems have new germplasm source (CMS-FA) male sterile cytoplasm background, are new germplasm source (CMS-FA)
The result that male sterile cytoplasm fertile gene interacts with new B systems nucleus fertile gene,
Completely by the heredity and expression of nucleo-cytoplasmic interreaction co- controlling male-sterile character.The pollen of new A systems
Sterility changes unrelated with nature illumination length and temperature height.
(3) the ecological reflection of new A systems male sterility and expression mechanism stabilization：With the temperature sensitive core of former light
Sterile line parent compare, and new A systems equally show as pollen sterility, but the ecology of pollen fertility
Reflection and expression mechanism have produced the entirely different change of property.The pollen sterility of new A systems
Will not with nature illumination length and the change of temperature height, generation can educate change, it is but in office
Under what weather conditions, height, the male sterility property of stabilization are remained.
(4) new A systems production application system is ripe sane：New A systems are protected with the new new germplasm source being bred as
Hold and be or the plantation of new B systems () interval, after heading, the pollen of new B systems awards new A systems, new A
It is that seed solid on plant remains as new A systems, new A systems is bred with the method；New A systems
Male sterile restoring line with new germplasm source (CMS-FA) system is spaced plantation, restorer plant pollen
New A systems are imparted to, solid seed is hybrid rice seed on new A systems plant.New A systems
The production application side of the breeding of sterile line and production of hybrid seeds production application method similar to wild abortion type sterile series
Method.Thus, new A systems production application system can be by means of traditional Yebai Three-line Hybrid rice
Production technology system, it is ripe and sane, protected for hybrid paddy rice production of hybrid seeds production provides solid new technology
Barrier.
Following examples are merely to illustrate and the purpose of unrestricted the application scope.
Rice plant material information used in this application can be found in rice in China kind and its
Pedigree database (http://www.ricedata.cn/variety/index.htm).
Embodiment 1Photo-thermo-sensitive genic male sterile line is extensively accounted for 63-4s orientations transformation Cheng Guang and accounts for 63-4A
The present embodiment is illustrated so that photo-thermo-sensitive genic male sterile line extensively accounts for 63-4s as an example, specifically and directed it
The method that transformation extensively accounts for 63-4A into new germplasm source male sterile line, details are as follows.
The first step, the seed selection of the homotype fertile line of photo-thermo sensitive male nuclear sterile rice line.
Extensively to account for 63-4s as female parent, early Xian kinds Zhejiang 987 as male parent, phase mutual cross,
Obtain hybrid seed.Plantation hybrid seed, obtains F1 generation plant.
Extensively to account for 63-4s as female parent, it is returned with the F1 generation plant as male parent, is obtained
Backcrossing seed.Plantation backcrossing seed, obtains B1F1 for plant.
Extensively to account for 63-4s as female parent, it is returned with the B1F1 plant as male parent, is obtained
Backcrossing seed.Plantation backcrossing seed, obtains B2F1 for plant.
Extensively to account for 63-4s as female parent, it is returned with the B2F1 plant as male parent, is obtained
Backcrossing seed.Plantation backcrossing seed, obtains B3F1 for plant.
Extensively to account for 63-4s as female parent, it is returned for plant with the B3F1 as male parent, is obtained
Seed must be returned.Plantation backcrossing seed, obtains B4F1 for plant.
Extensively to account for 63-4s as female parent, it is returned with the B4F1 plant as male parent, is obtained
Backcrossing seed.Plantation backcrossing seed, obtains B5F1 for plant.
Extensively to account for 63-4s as female parent, it is returned with the B5F1 plant as male parent, is obtained
Backcrossing seed.Plantation backcrossing seed, obtains B6F1 for plant.
B6F1 is treated for its selfing of plant heading relief, harvesting wherein pollen and small ear can educate completely
The selfed seed of plant 2000.Plantation selfed seed, obtains B6F2 for 1000 plants of plant.
Treat that B6F2, for its selfing of plant heading relief, selects pollen and Spikelet fertility complete after maturation
Full normal plant, point individual plant harvests 50 plants of selfed seeds.Plantation selfed seed, obtains B6F3
For 50 strains of plant, each strain plants 100 plants.
Treat that B6F3, for its selfing of plant heading relief, selects whole plant in cell after maturation
Pollen and small ear can normally educate completely, be separated without sterile plant, complex inheritance background and comprehensive agronomy
The proterties strain B6F3-1 similar to rice photo-thermo-sensitive sterile line, i.e. photo-thermo sensitive male nuclear sterile rice line
Homotype fertile line, be named as and extensively accounted for 63-4B systems.
Second step, the seed selection of protection (CMS-FA) cytoplasmic male sterile line.
Using the new source 5A of new germplasm source (CMS-FA) sterile line as female parent, the conduct of 63-4B systems is extensively accounted for
Male parent, phase mutual cross obtains hybrid seed.Plantation hybrid seed, obtains F1 generation plant.
Using F1 generation plant as female parent, account for 63-4B systems and be returned with as the wide of male parent,
Obtain backcrossing seed.Plantation backcrossing seed, obtains B1F1 plant.
Using B1F1 for plant as female parent, account for 63-4B systems and be returned with as the wide of male parent,
Obtain backcrossing seed.Plantation backcrossing seed, obtains B2F1 plant.
Using B2F1 for plant as female parent, account for 63-4B systems and be returned with as the wide of male parent,
Obtain backcrossing seed.Plantation backcrossing seed, obtains B3F1 plant.
Using B3F1 for plant as female parent, account for 63-4B systems and be returned with as the wide of male parent,
Obtain backcrossing seed.Plantation backcrossing seed, obtains B4F1 plant.
Using B4F1 for plant as female parent, account for 63-4B systems and be returned with as the wide of male parent,
Obtain backcrossing seed.Plantation backcrossing seed, obtains B5F1 plant.
Using B5F1 for plant as female parent, account for 63-4B systems and be returned with as the wide of male parent,
Obtain backcrossing seed.Plantation backcrossing seed, obtains B6F1 plant, is named as and extensively accounts for 63-4A
System.
The wide tree characteristics for accounting for 63-4A systems is neat, stabilization, and complex inheritance background accounts for 63-4B with extensively
It is similar, comprehensive agronomy proterties similar to the wide 63-4B systems that account for, but pollen and small ear are completely not
Educate, extensively account for 63-4A systems and be new new germplasm source (CMS-FA) male sterile line being bred as, that is,
Photo-thermo sensitive male nuclear sterile rice line orient transformation into new lines, i.e. protection (CMS-FA) cytoplasm
Male sterile line.
The wide the key technical indexes for accounting for 63-4A systems is as follows：It is seeded into beginning fringe 85 days, is broadcast with the same period
Plant the wide of rice transplanting and account for 63-4s, breeding time is suitable；It is wide to account for 63-4A beginnings ear period detection pollen fertility,
Based on typical abortion.More than 1000 plants colony's sterile plant rates 100%, pollen sterility degree
99.98%, bagging self-fruitful rate is 0%.
Extensively accounting for 63-4A rice quality testing results is：Coarse Rice Rate 80.9%, polished rice rate 72.4%,
Head rice rate 60.3%, 6.3 millimeters of grain length, length-width ratio 2.9, chalky grain rate 14.2%, chalk is white
Degree 3.0%, 1 grade of transparency, 6.0 grades of caustic SCC, 87.3 millimeters of gel consistence, amylose contains
Amount 15.7%, rice quality totally reaches national Prime-3 rating rice, 2 grades of rice of new ministerial level high-quality.
Embodiment 2Photo-thermo-sensitive genic male sterile line is extensively accounted for 63-4s and orients transformation into pursuit A
Rice photo-thermo-sensitive male sterility line is extensively accounted for 63-4s orientation transformations Cheng Xinzhi by the present embodiment
Source (CMS-FA) male sterile line pursues A, wherein pursuing A equivalent to wide in for embodiment 1
63-4A is accounted for, extensively 63-4B is accounted in pursuing B equivalent to embodiment 1, the number of times being simply returned
Different from embodiment 1, Breeding direction is consistent with result.
1st, the wide 63-4s that accounts for orients transformation into pursuit A：
Hybridized with Zhejiang 987 using extensively 63-4s is accounted for, and repeatedly returned as recurrent parent with extensively accounting for 63-4s
Hand over and selfing, the pursuit B that pedigree seed selection is obtained is cell nucleus donor, while using hybridization and returning
The method of friendship, the synchronous cytoplasm for importing the new source 5A of new germplasm source (CMS-FA) sterile line, is bred as
Proterties is neatly stablized, the new germplasm source (CMS-FA) that sterile degree is high, outcrossing rate is high, rice quality is excellent
Sterile line pursues A.
Detailed process is as shown in table 1, wherein combining male parent (maintainer) seed selection and maternal (infertility
System) seed selection 2 sets work, male parent seed selection referring to the 5th arrange, maternal seed selection is referring to secondary series.
Table 1 pursues the Breeding Process of A
Note："/" or "×" represent hybridization or are returned；"/numerical value/" represents hybridization or backcrossing number of times；F is represented
Hybridization；B represents backcrossing；N represents backcross generations number.
2nd, A is pursued to account for 63-4s botanical characters and compare with wide：
Pursue A to account for 63-4s and compare with wide, in plant height, spike length, number of grain per ear, mass of 1000 kernel, broadcast
The botanical characters such as kind-beginning fringe, rice quality aspect substantially similar (table 2).
Table 2 pursues A and accounts for 63-4s botanical characters and compare with wide
3rd, A is pursued to account for 63-4s biological characteristicses and compare with wide：
Pursue A and extensively account for 63-4s and compare with compareing, it is steady in male sterility set type, male sterility
It is qualitative, whether need maintainer, restorer source etc. biological characteristics aspect there is notable difference
(table 3).
Table 3 pursues A and accounts for 63-4s biological characteristicses and compare with wide
Embodiment 3The numerous 8s of photo-thermo-sensitive genic male sterile line is oriented into transformation into numerous 8A
The present embodiment by taking the numerous 8s of photo-thermo-sensitive genic male sterile line as an example, specifically illustrate direct it transformation into
The method of the numerous 8A of new germplasm source male sterile line, details are as follows.
The first step, the seed selection of the homotype fertile line of photo-thermo sensitive male nuclear sterile rice line.
Using numerous 8s as female parent, early Xian kinds Zhejiang 987 obtains miscellaneous as male parent, phase mutual cross
Hand over seed.Plantation hybrid seed, obtains F1 generation plant.
Using numerous 8s as female parent, it is returned with the F1 generation plant as male parent, is returned
Seed.Plantation backcrossing seed, obtains B1F1 for plant.
Using numerous 8s as female parent, it is returned with the B1F1 plant as male parent, is returned
Seed.Plantation backcrossing seed, obtains B2F1 for plant.
Using numerous 8s as female parent, it is returned with the B2F1 plant as male parent, is returned
Seed.Plantation backcrossing seed, obtains B3F1 for plant.
Using numerous 8s as female parent, it is returned for plant with the B3F1 as male parent, is returned
Hand over seed.Plantation backcrossing seed, obtains B4F1 for plant.
Using numerous 8s as female parent, it is returned with the B4F1 plant as male parent, is returned
Seed.Plantation backcrossing seed, obtains B5F1 for plant.
Using numerous 8s as female parent, it is returned with the B5F1 plant as male parent, is returned
Seed.Plantation backcrossing seed, obtains B6F1 for plant.
Using numerous 8s as female parent, it is returned with the B6F1 plant as male parent, is returned
Seed.Plantation backcrossing seed, obtains B7F1 for plant.
B7F1 is treated for its selfing of plant heading relief, harvesting wherein pollen and small ear can educate completely
The selfed seed of plant 2500.Plantation selfed seed, obtains B7F2 for 800 plants of plant.
Treat that B7F2, for its selfing of plant heading relief, selects pollen and Spikelet fertility complete after maturation
Full normal plant, point individual plant harvests 50 plants of selfed seeds.Plantation selfed seed, obtains B7F3
For 40 strains of plant, each strain plants 100 plants.
Treat that B7F3, for its selfing of plant heading relief, selects whole plant in cell after maturation
Pollen and small ear can normally educate completely, be separated without sterile plant, complex inheritance background and comprehensive agronomy
The proterties strain B7F3-1 similar to rice photo-thermo-sensitive sterile line, as rice photo-thermo-sensitive sterile line
Homotype fertile line, be named as numerous 8B systems.
Second step, the seed selection of protection (CMS-FA) cytoplasmic male sterile line.
Using the new source 5A of new germplasm source (CMS-FA) sterile line as female parent, numerous 8B systems as male parent,
Phase mutual cross, obtains hybrid seed.Plantation hybrid seed, obtains F1 generation plant.
Using F1 generation plant as female parent, it is returned with the numerous 8B systems as male parent, is obtained
Backcrossing seed.Plantation backcrossing seed, obtains B1F1 plant.
Using B1F1 for plant as female parent, it is returned with the numerous 8B systems as male parent, is obtained
Seed must be returned.Plantation backcrossing seed, obtains B2F1 plant.
Using B2F1 for plant as female parent, it is returned with the numerous 8B systems as male parent, is obtained
Seed must be returned.Plantation backcrossing seed, obtains B3F1 plant.
Using B3F1 for plant as female parent, it is returned with the numerous 8B systems as male parent, is obtained
Seed must be returned.Plantation backcrossing seed, obtains B4F1 plant.
Using B4F1 for plant as female parent, it is returned with the numerous 8B systems as male parent, is obtained
Seed must be returned.Plantation backcrossing seed, obtains B5F1 plant, is named as numerous 8A systems.
The tree characteristics of numerous 8A systems is neat, stabilization, complex inheritance background is similar to numerous 8B systems,
Comprehensive agronomy proterties is similar to numerous 8B systems, but pollen and small ear are completely sterile, and numerous 8A systems are
It is that new protection (CMS-FA) cytoplasmic male sterile line being bred as or new germplasm source (CMS-FA) are male
Property sterile line.
Numerous 8A the key technical indexes is as follows：Beginning fringe is seeded into 81 days, with same period sowing rice transplanting
Numerous 8s breeding times are suitable；Numerous 8A beginnings ear period detects pollen fertility, based on typical abortion.1000
Strain above colony sterile plant rate 100%, pollen sterility degree 99.97%, bagging self-fruitful rate is
0%.
Numerous 8A rice qualities testing result is：Rice quality is general.
Embodiment 4By the double 8s orientation transformations of photo-thermo-sensitive genic male sterile line 8A in pairs
The present embodiment by taking the double 8s of photo-thermo-sensitive genic male sterile line as an example, specifically illustrate direct it transformation into
The method of the double 8A of new germplasm source male sterile line, details are as follows.
The first step, the seed selection of the homotype fertile line of photo-thermo sensitive male nuclear sterile rice line.
Using double 8s as female parent, early Xian kinds Zhejiang 987 obtains miscellaneous as male parent, phase mutual cross
Hand over seed.Plantation hybrid seed, obtains F1 generation plant.
Using double 8s as female parent, it is returned with the F1 generation plant as male parent, is returned
Seed.Plantation backcrossing seed, obtains B1F1 for plant.
Using double 8s as female parent, it is returned with the B1F1 plant as male parent, is returned
Seed.Plantation backcrossing seed, obtains B2F1 for plant.
Using double 8s as female parent, it is returned with the B2F1 plant as male parent, is returned
Seed.Plantation backcrossing seed, obtains B3F1 for plant.
Using double 8s as female parent, it is returned for plant with the B3F1 as male parent, is returned
Hand over seed.Plantation backcrossing seed, obtains B4F1 for plant.
Using double 8s as female parent, it is returned with the B4F1 plant as male parent, is returned
Seed.Plantation backcrossing seed, obtains B5F1 for plant.
Using double 8s as female parent, it is returned with the B5F1 plant as male parent, is returned
Seed.Plantation backcrossing seed, obtains B6F1 for plant.
B6F1 is treated for its selfing of plant heading relief, harvesting wherein pollen and small ear can educate completely
The selfed seed of plant 1800.Plantation selfed seed, obtains B6F2 for 1200 plants of plant.
Treat that B6F2, for its selfing of plant heading relief, selects pollen and Spikelet fertility complete after maturation
Full normal plant, point individual plant harvests 50 plants of selfed seeds.Plantation selfed seed, obtains B6F3
For 50 strains of plant, each strain plants 80 plants.
Treat that B6F3, for its selfing of plant heading relief, selects whole plant in cell after maturation
Pollen and small ear can normally educate completely, be separated without sterile plant, complex inheritance background and comprehensive agronomy
The proterties strain B6F3-1 similar to rice photo-thermo-sensitive sterile line, as rice photo-thermo-sensitive sterile line
Homotype fertile line, be named as double 8B systems.
Second step, the seed selection of protection (CMS-FA) cytoplasmic male sterile line.
Using the new source 5A of new germplasm source (CMS-FA) sterile line as female parent, double 8B systems as male parent,
Phase mutual cross, obtains hybrid seed.Plantation hybrid seed, obtains F1 generation plant.
Using F1 generation plant as female parent, it is returned with the double 8B systems as male parent, is obtained
Backcrossing seed.Plantation backcrossing seed, obtains B1F1 plant.
Using B1F1 for plant as female parent, it is returned with the double 8B systems as male parent, is obtained
Seed must be returned.Plantation backcrossing seed, obtains B2F1 plant.
Using B2F1 for plant as female parent, it is returned with the double 8B systems as male parent, is obtained
Seed must be returned.Plantation backcrossing seed, obtains B3F1 plant.
Using B3F1 for plant as female parent, it is returned with the double 8B systems as male parent, is obtained
Seed must be returned.Plantation backcrossing seed, obtains B4F1 plant.
Using B4F1 for plant as female parent, it is returned with the double 8B systems as male parent, is obtained
Seed must be returned.Plantation backcrossing seed, obtains B5F1 plant.
Using B5F1 for plant as female parent, it is returned with the double 8B systems as male parent, is obtained
Seed must be returned.Plantation backcrossing seed, obtains B6F1 plant.
Using B6F1 for plant as female parent, it is returned with the double 8B systems as male parent, is obtained
Seed must be returned.Plantation backcrossing seed, obtains B7F1 plant, is named as double 8A systems.
The tree characteristics of double 8A systems is neat, stabilization, complex inheritance background, comprehensive agronomy proterties
It is similar to double 8B systems, but pollen and small ear are completely sterile, and double 8A systems are the wide of new incubation
Guarantor's type (CMS-FA) cytoplasmic male sterile line or new germplasm source (CMS-FA) male sterile line.
Double 8A the key technical indexes are as follows：Beginning fringe is seeded into 80 days, with same period sowing rice transplanting
Double 8s breeding times are suitable；Double 8A beginnings ear periods detect pollen fertility, based on typical abortion.1000
Strain above colony sterile plant rate 100%, pollen sterility degree 99.96%, bagging self-fruitful rate is
0%.
Double 8A rice qualities testing results are：Rice quality is general.
Claims (10)
1. photo-thermo sensitive male nuclear sterile rice line orients transformation into the side of protection cytoplasmic male sterile line
Method, it is comprised the following steps：
Maternal by backcrossing of photo-thermo sensitive male nuclear sterile rice line, rice-cultivating is male parent, by hybridizing,
Backcrossing and selfing, seed selection obtain the homotype fertile line of photo-thermo sensitive male nuclear sterile rice line.
2. the method for claim 1, its is further comprising the steps of：
It is backcrossing male parent with the homotype fertile line of the photo-thermo sensitive male nuclear sterile rice line, paddy rice extensively protects
Type cytoplasmic male sterile line is female parent, and by hybridizing and being returned, seed selection obtains genetic property and changes
The rice new strain of change, i.e., the new protection cytoplasmic male sterile line being bred as.
3. method as claimed in claim 2, wherein the genetic property changes includes paddy rice
Sterility is to illumination and temperature-insensitive.
4. method as claimed any one in claims 1 to 3, wherein the seed selection obtains water
The step of homotype fertile line of rice photo-thermo-sensitive nucleic male sterile line, includes 2-8 backcrossing and 3-5 selfing,
It is preferred that 4-7 backcrossing and 4 selfings, more preferably 6 times backcrossings and 4 selfings.
5. the method as any one of Claims 1-4, wherein the seed selection is lost
The step of rice new strain for passing attribute change, is returned including 2-8 times, preferably 4-7 times backcrossing,
More preferably 6 times backcrossings.
6. the method as any one of claim 1 to 5, wherein the rice photo-thermo-sensitive
Line with genic sterile is：Light high temperature-induced sterility type line with genic sterile long, high temperature-induced sterility type line with genic sterile, short light
Low temperature infertility type line with genic sterile or low temperature infertility type line with genic sterile；Optionally, the paddy rice light
Temperature-sensitive sterile line is extensively to account for 63-4s, numerous 8s or double 8s.
7. the method as any one of claim 1 to 6, wherein the paddy rice protection
Cytoplasmic male sterile line is new source 5A, peasants who dig gold 1A, peasants who dig gold 2A or peasants who dig gold 3A.
8. the method as any one of claim 1 to 7, wherein methods described include with
Lower step：
1) 63-4s is extensively accounted for as maternal with rice photo-thermo-sensitive sterile line, using itself and the cultivation as male parent
Training paddy rice Zhejiang 987 is hybridized, and obtains cenospecies；
2) extensively to account for 63-4s with rice photo-thermo-sensitive sterile line maternal as backcrossing, using its with as male parent
The step of 1) cenospecies that is obtained is returned, continues to be returned about 6 times, obtains backcrossing kind；
And
3) by step 2) the backcrossing kind that is obtained carries out 3-5 selfing, the paddy rice light that seed selection is obtained
The homotype fertile line of temperature-sensitive sterile line extensively accounts for 63-4B.
9. method as claimed in claim 8, further comprising the steps of：
4) 63-4B is extensively accounted for as male parent with homotype fertile line, it is extensively protected with as maternal paddy rice
The new source 5A of type cytoplasmic male sterile line is hybridized, and obtains cenospecies；And
5) 63-4B as backcrossing male parent is extensively accounted for homotype fertile line, using itself and the step as female parent
4) cenospecies for being obtained is returned, and continues to be returned about 6 times, and seed selection obtains genetic property and changes
The rice new strain of change, i.e., the new protection cytoplasmic male sterile line being bred as extensively accounts for 63-4A.
10. method as claimed in any one of claims 1-9 wherein, wherein the paddy rice light temperature
Quick line with genic sterile, the homotype fertile line of the photo-thermo sensitive male nuclear sterile rice line or the heredity category
The rice new strain for sexually revising is selected from paddy rice, upland rice, long-grained nonglutinous rice, japonica rice, early rice, late rice, viscous
Rice, glutinous rice；
Optionally, the photo-thermo sensitive male nuclear sterile rice line, the photo-thermo sensitive male nuclear sterile rice line
The rice new strain that homotype fertile line or the genetic property change is selected from rice leaf hairiness rice and rice
Leaf is without hair rice.
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