CN106889298A - A kind of fructus cannabis polypeptide powder and preparation method thereof - Google Patents
A kind of fructus cannabis polypeptide powder and preparation method thereof Download PDFInfo
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- CN106889298A CN106889298A CN201510952402.6A CN201510952402A CN106889298A CN 106889298 A CN106889298 A CN 106889298A CN 201510952402 A CN201510952402 A CN 201510952402A CN 106889298 A CN106889298 A CN 106889298A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/14—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds
- A23J1/148—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds by treatment involving enzymes or microorganisms
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Abstract
The invention provides a kind of fructus cannabis polypeptide powder and preparation method thereof, the invention belongs to plant polypeptide pink collar domain.The polypeptide powder is the full nutrition polypeptide powder being prepared from using modern crafts means with fructus cannabis as raw material.The present invention is by the species and the research and probe of enzymatic hydrolysis condition to enzyme, screening obtains the enzyme and enzymatic hydrolysis condition that can avoid producing bitter peptides, establish a set of technique for producing polypeptide powder through protein extraction, grease separation, protein salvage, enzymolysis, drying, and gone out the fructus cannabis polypeptide powder that enzymolysis degree is high, mouthfeel is full using this technique productions, so that nutrition and health care effect of fructus cannabis is not fully exerted.Flavoring essence can be also added in polypeptide powder raw material of the present invention to adjust mouthfeel.In view of this polypeptide powder have dissolubility it is good, need not digestion is decomposed, to absorb rapid, absorption efficiency high, and in absorption process need not power consumption the features such as, be especially suitable for that digestive function is poor and crowd in poor health.
Description
Technical field
The invention belongs to plant polypeptide pink collar domain, the invention provides a kind of fructus cannabis polypeptide powder and its preparation side
Method.
Background technology
China is protein production state and country of consumption maximum in the world, is also fastest-rising global albumen industry
Country.In the production and consumption of protide product, polypeptide powder is welcome by consumer deeply in recent years.At present,
The common polypeptide powder kind of in the market has soyabean polypeptide powder, walnut polypeptide powder and Peanut Polypeptide powder etc., market pair
Plant polypeptide powder future development is held a optimistic attitude, however, plant polypeptide powder industry is also faced with one in development
The problem of series, such as single varieties, backward in technique, Market competition, compared with developed countries, I
The plant polypeptide powder process technology of state and Foreign Advanced Lerel still have a larger gap, such as absorption efficiency it is low,
Auxiliary material adds excessive etc..Additionally, at present China plant polypeptide powder category be also limited only to peanut, soybean,
, seldom there is the product of other raw materials in several kinds few in number such as walnut.
Fructus cannabis has the edible history of thousands of years as a kind of plant kernel of medicine-food two-purpose in China, in recent years
Come, many scientific research institutions all conduct in-depth research to its nutritional ingredient and healthcare function, obtain full and accurate
Achievement in research.Research is confirmed：Fructus cannabis contain about 25%~35% grease, 20%~25% protein,
20%~30% dietary fiber, and abundant vitamin and mineral matter.Protein content in fructus cannabis is very
Height, mainly includes edestan albumen and edestan albumin, and 65% is edestin, the albumen in edestan albumen
Digestion can be promoted, be the skeleton raw material of cell DNA, 35% is albumin in addition, and it contains needed by human body
Various essential amino acids, in edestan protein contain substantial amounts of arginine and glutamic acid, meanwhile, have content ratio
Cystine higher, methionine and histidine.Wherein, arginine and histidine rise in upgrowth and development of children
Important function, arginine easily produces NO in blood vessel, and this has very much for middle-aged and old prevention of cardiovascular diseases
Help, additionally, containing branched-chain amino acid relatively high in edestan protein, for becoming thin, crowd recovers constitution
It is of great advantage.On the other hand, edestan protein is free of tryptophan inhibiting factor, does not interfere with the suction of protein
Receive, also not soy-containing some oligosaccharides and sensitizing factor, therefore it is anti-not result in gasteremphraxis, gastric disorder causing nausea and allergy
Should.Fructus cannabis do not have bitter taste compared with other plant albumen, good in taste.To sum up, fructus cannabis egg
White various features show that fructus cannabis can be as a kind of desirable feedstock of novel plant polypeptide powder.Except this it
Outward, also containing abundant mineral matter in fructus cannabis, including phosphorus, potassium, magnesium, calcium, sulphur, iron, zinc etc., this
Secondary element is all coenzyme composition necessary to fatty acid metabolism slightly.In fructus cannabis also rich in choline, volatile oil,
Many nutritional ingredients such as vitamin B1.
Fructus cannabis has many health-care efficacies.China's traditional medicine thinks that fructus cannabis sweet taste has ease constipation
Defaecation, the effect of nourishing Yin and promoting production of body fluid, are clinically usually used in the diseases such as treatment blood deficiency and depleted fluid, dry constipation of intestines.Modern pharmacology
Learn research and show that fructus cannabis has preferable hypotensive, diuresis, analgesia, anti-inflammatory, anti-thrombosis function,
Long-term consumption, to chronic forms inflammation, paralysis, constipation, hypertension, high fat of blood, digestive tract ulcer and gynaecology
Disease has good auxiliary curative effect.
In sum, fructus cannabis has good development prospect, however, fructus cannabis fruit is due to having
Rigid shell, is not easy to directly eat, therefore, it is badly in need of a kind of processing method that can extract full nutrition, this
The method of invention be exactly will wherein contain Major Nutrient material --- Protein Extraction is out prepared into albumen powder
Or polypeptide powder, and retain the other active components such as mineral matter, vitamin as far as possible.We are by deeply
Research find that isoelectric points of proteins is wide in range in fructus cannabis, and the albumen powder dissolubility prepared is poor, it is necessary to it is sharp
Appropriate enzymolysis is carried out to protein therein with modern biotechnology, the preferable polypeptide powder of dissolubility is obtained,
Further improve the absorbent properties of product.
It is well known that compared with protein, polypeptide has dissolubility good, rapid without digestion decomposition, absorption
And absorption efficiency it is high the characteristics of, crowd especially weaker to those digestive functions, these advantages of polypeptide just show
Obtain more important.However, during being polypeptide by protein digestion, bitter peptides often occur, this
Planting bitter taste is caused by the hydrophobic amino acid in polypeptide, and generally the amino acid structure composition with protein is relevant,
Polypeptide hydrophobic deg, amino acid sequence and space structure are important influence factors.Therefore, enzymolysis egg is being carried out
During white matter raw material production polypeptide products, the selection of the species and enzymatic hydrolysis condition of enzyme is most important.
The present invention by the species and the research and probe of enzymatic hydrolysis condition to enzyme, establish it is a set of through protein extraction,
The technique of polypeptide powder is produced in grease separation, protein salvage, enzymolysis, drying, and is gone out using this technique productions
The fructus cannabis polypeptide powder that content of peptides is high, dissolubility is good, enzymolysis degree is high, in good taste, so that fructus cannabis
Nutrition and health care effect is not fully exerted.
Fructus cannabis polypeptide powder of the present invention mainly possesses following technical characterstic：1. established by research and carried through albumen
Take, the technique of polypeptide powder is produced in grease separation, protein salvage, enzymolysis, drying, screening is obtained and can kept away
Exempt from the enzyme and enzymatic hydrolysis condition of generation bitter peptides；2. with the polypeptide powder such as current peanut, soybean mainly using remaining after oil expression
The dregs of rice for staying carry out protein extraction and compare, and the holoprotein that the present invention realizes the full benevolence of fructus cannabis is extracted, so as to simplify
Technological process, while avoiding the loss of nutriment, reduces the generation of protein denaturation, as far as possible
The bioactivity for maintaining active ingredient in fructus cannabis and improve active ingredient absorption in vivo, most
The quality of product is ensure that in big degree；3. this product belongs to a kind of full nutrition polypeptide powder, substantially maintains fire
Nutriment ratio in a balanced way in edestan fruit；4. compared with traditional protein powder, this product dissolubility is good, need not
Digestion is decomposed, absorption is rapid, absorption efficiency is high, and without power consumption in absorption process, for following crowd
With more importantly meaning：1. digestive system defect, obstacle, damage and nutrition person can not be absorbed, 2. transport
Moving group nitrogen source and can not increase gastrointestinal function bearer being badly in need of supplement, and 3. digestion power is poor, nutrition is scarce
Weary, in poor health, post-operative recovery patient；5. this product has good mouthfeel, is suitable for various types of disappearing
Fei Zhe；6. the Related product similar with this product there is no in the market.To sum up, it can be seen that of the invention
Using and popularization extremely meet the current market demand.
The content of the invention
It is an object of the invention to provide a kind of fructus cannabis polypeptide powder and preparation method thereof.
Fructus cannabis polypeptide powder of the invention it is characterized in that：It is fire fiber crops to be made the raw material of the fructus cannabis polypeptide powder
Benevolence, the fructus cannabis polypeptide powder is prepared from accordance with the following methods：
1) fructus cannabis raw material is weighed, the drinking water for adding temperature to be 40-90 DEG C, it is 1: 6-1: 20 to be made into solid-liquid ratio
Suspension, mashing, suspension was obtained successively with the screen filtration of 40-200 mesh and 200-400 mesh after crushing
Filtrate；
2) filtered fluid that 1) step is obtained is placed in a centrifuge and is centrifuged 5-20 minutes in 2000-4000r/min,
It is divided into three layers after centrifugation, upper strata is oil layer, the oil layer is toppled over into removal, middle level is supernatant layer, lower floor
It is the beds of precipitation, supernatant layer and the beds of precipitation retain standby；
3) add 37% hydrochloric acid that its pH value is adjusted into 3-6 in the supernatant layer for obtaining to 2) step, place 10-60
It is placed in a centrifuge after minute and is centrifuged 5-20 minutes in 2000-5000r/min, gained is heavy after centrifugate is filtered
The lower sediment layer that shallow lake obtains with 2) step mixes, and obtains mixed precipitation；
4) the 5-20 times of drinking water measured is added to obtain constant volume liquid in the mixed precipitation for obtaining to 3) step, to constant volume
After adding NaOH that its pH value is adjusted into 8-12 in liquid, the basic protein of 0.05%-5% is added thereto to
Enzyme, is placed in 40-80 DEG C of environment and digests 1-5 hours, obtains digesting suspension；
5) the enzymolysis suspension for obtaining 4) step is placed in a centrifuge in 5-20 points of 2000-4000r/min centrifugations
Clock, is divided into two-layer after centrifugation, upper strata is supernatant layer, and lower floor is the beds of precipitation, takes out supernatant liquor and is placed on
Dried in 40-80 DEG C of environment 3-24 hours, moisture obtains meal after being evaporated, the meal obtains final product this after crushed
Invention fructus cannabis polypeptide powder.
Further, flavoring essence is also included in the raw material of fructus cannabis polypeptide powder of the present invention, containing flavoring essence
Fructus cannabis polypeptide powder is prepared from accordance with the following methods：
1) fructus cannabis raw material is weighed, the drinking water for adding temperature to be 40-90 DEG C, it is 1: 6-1: 20 to be made into solid-liquid ratio
Suspension, mashing, suspension was obtained successively with the screen filtration of 40-200 mesh and 200-400 mesh after crushing
Filtrate；
2) filtered fluid that 1) step is obtained is placed in a centrifuge and is centrifuged 5-20 minutes in 2000-4000r/min,
It is divided into three layers after centrifugation, upper strata is oil layer, the oil layer is toppled over into removal, middle level is supernatant layer, lower floor
It is the beds of precipitation, supernatant layer and the beds of precipitation retain standby；
3) add 37% hydrochloric acid that its pH value is adjusted into 3-6 in the supernatant layer for obtaining to 2) step, place 10-60
It is placed in a centrifuge after minute and is centrifuged 5-20 minutes in 2000-5000r/min, gained is heavy after centrifugate is filtered
The lower sediment layer that shallow lake obtains with 2) step mixes, and obtains mixed precipitation；
4) the 5-20 times of drinking water measured is added to obtain constant volume liquid in the mixed precipitation for obtaining to 3) step, to constant volume
After adding NaOH that its pH value is adjusted into 8-12 in liquid, the basic protein of 0.05%-5% is added thereto to
Enzyme, is placed in 40-80 DEG C of environment and digests 1-5 hours, obtains digesting suspension；
5) the enzymolysis suspension for obtaining 4) step is placed in a centrifuge in 5-20 points of 2000-4000r/min centrifugations
Clock, is divided into two-layer after centrifugation, upper strata is supernatant layer, and lower floor is the beds of precipitation, takes out supernatant liquor and is placed on
Dried in 40-80 DEG C of environment 3-24 hours, moisture obtains meal after being evaporated, the meal obtains final product fire after crushed
Edestan polypeptide powder；
6) the fructus cannabis polypeptide powder for obtaining 5) step mixes with the flavoring essence of 0.1%-0.8%, crushes homogeneous
The fructus cannabis polypeptide powder containing flavoring essence is obtained final product afterwards.
Further, fructus cannabis polypeptide powder of the present invention, in its raw material contained flavoring essence preferably apple essence or
Walnut essence.
Further, the preparation method of fructus cannabis polypeptide powder of the present invention, it is characterised in that comprise the following steps：
1) weigh fructus cannabis raw material, the drinking water for adding temperature to be 70 DEG C, be made into solid-liquid ratio for 1: 10 it is outstanding
Liquid, mashing, suspension obtains filtered fluid successively with 100 mesh and the screen filtration of 300 mesh after crushing；
2) filtered fluid that 1) step is obtained is placed in a centrifuge and is centrifuged 10 minutes in 3000r/min, after centrifugation
It is divided into three layers, upper strata is oil layer, the oil layer is toppled over into removal, middle level is supernatant layer, and lower floor is precipitation
Layer, supernatant layer and the beds of precipitation retain standby；
3) add 37% hydrochloric acid that its pH value is adjusted into 4.5 in the supernatant layer for obtaining to 2) step, place 30
Minute after be placed in a centrifuge in 4000r/min be centrifuged 10 minutes, after centrifugate is filtered gained precipitation with 2)
The lower sediment layer mixing that step is obtained, obtains mixed precipitation；
4) drinking water of 10 times of amounts are added to obtain constant volume liquid in the mixed precipitation for obtaining to 3) step, to constant volume liquid
After its pH value is adjusted to 9 by middle addition NaOH, 1% alkali protease is added thereto to, is placed in 60 DEG C
Environment in digest 2 hours, obtain digest suspension；
5) the enzymolysis suspension for obtaining 4) step is placed in a centrifuge and is centrifuged 10 minutes in 3000r/min, from
It is divided into two-layer after the heart, upper strata is supernatant layer, and lower floor is the beds of precipitation, takes out the ring that supernatant liquor is placed on 48 DEG C
Dried 12 hours in border, moisture obtains meal after being evaporated, and it is many that the meal obtains final product fructus cannabis of the present invention after crushed
Gly-His-Lys.
Further, the preparation method of fructus cannabis polypeptide powder of the present invention containing flavoring essence, it is characterised in that bag
Include following steps：
1) weigh fructus cannabis raw material, the drinking water for adding temperature to be 70 DEG C, be made into solid-liquid ratio for 1: 10 it is outstanding
Liquid, mashing, suspension obtains filtered fluid successively with 100 mesh and the screen filtration of 300 mesh after crushing；
2) filtered fluid that 1) step is obtained is placed in a centrifuge and is centrifuged 10 minutes in 3000r/min, after centrifugation
It is divided into three layers, upper strata is oil layer, the oil layer is toppled over into removal, middle level is supernatant layer, and lower floor is precipitation
Layer, supernatant layer and the beds of precipitation retain standby；
3) add 37% hydrochloric acid that its pH value is adjusted into 4.5 in the supernatant layer for obtaining to 2) step, place 30
Minute after be placed in a centrifuge in 4000r/min be centrifuged 10 minutes, after centrifugate is filtered gained precipitation with 2)
The lower sediment layer mixing that step is obtained, obtains mixed precipitation；
4) drinking water of 10 times of amounts are added to obtain constant volume liquid in the mixed precipitation for obtaining to 3) step, to constant volume liquid
After its pH value is adjusted to 9 by middle addition NaOH, 1% alkali protease is added thereto to, is placed in 60 DEG C
Environment in digest 2 hours, obtain digest suspension；
5) the enzymolysis suspension for obtaining 4) step is placed in a centrifuge and is centrifuged 10 minutes in 3000r/min, from
It is divided into two-layer after the heart, upper strata is supernatant layer, and lower floor is the beds of precipitation, takes out the ring that supernatant liquor is placed on 48 DEG C
Dried 12 hours in border, moisture obtains meal after being evaporated, and the meal obtains final product fructus cannabis polypeptide powder after crushed；
6) flavoring essence of the fructus cannabis polypeptide powder with 0.5% for obtaining 5) step mixes, and is obtained final product after crushing homogeneous
Fructus cannabis polypeptide powder containing flavoring essence.
Specific embodiment
Embodiment 1
Raw material：Fructus cannabis
Preparation method is as follows：
1) weigh fructus cannabis raw material, the drinking water for adding temperature to be 70 DEG C, be made into solid-liquid ratio for 1: 10 it is outstanding
Liquid, mashing, suspension obtains filtered fluid successively with 100 mesh and the screen filtration of 300 mesh after crushing；
2) filtered fluid that 1) step is obtained is placed in a centrifuge and is centrifuged 10 minutes in 3000r/min, after centrifugation
It is divided into three layers, upper strata is oil layer, the oil layer is toppled over into removal, middle level is supernatant layer, and lower floor is precipitation
Layer, supernatant layer and the beds of precipitation retain standby；
3) add 37% hydrochloric acid that its pH value is adjusted into 4.5 in the supernatant layer for obtaining to 2) step, place 30
Minute after be placed in a centrifuge in 4000r/min be centrifuged 10 minutes, after centrifugate is filtered gained precipitation with 2)
The lower sediment layer mixing that step is obtained, obtains mixed precipitation；
4) drinking water of 10 times of amounts are added to obtain constant volume liquid in the mixed precipitation for obtaining to 3) step, to constant volume liquid
After its pH value is adjusted to 9 by middle addition NaOH, 1% alkali protease is added thereto to, is placed in 60 DEG C
Environment in digest 2 hours, obtain digest suspension；
5) the enzymolysis suspension for obtaining 4) step is placed in a centrifuge and is centrifuged 10 minutes in 3000r/min, from
It is divided into two-layer after the heart, upper strata is supernatant layer, and lower floor is the beds of precipitation, takes out the ring that supernatant liquor is placed on 48 DEG C
Dried 12 hours in border, moisture obtains meal after being evaporated, and it is many that the meal obtains final product fructus cannabis of the present invention after crushed
Gly-His-Lys.
Embodiment 2
Raw material：Fructus cannabis
Preparation method is as follows：
1) weigh fructus cannabis raw material, the drinking water for adding temperature to be 40 DEG C, be made into solid-liquid ratio for 1: 20 it is outstanding
Liquid, mashing, suspension obtains filtered fluid successively with 200 mesh and the screen filtration of 400 mesh after crushing；
2) filtered fluid that 1) step is obtained is placed in a centrifuge and is centrifuged 20 minutes in 2000r/min, after centrifugation
It is divided into three layers, upper strata is oil layer, the oil layer is toppled over into removal, middle level is supernatant layer, and lower floor is precipitation
Layer, supernatant layer and the beds of precipitation retain standby；
3) add 37% hydrochloric acid that its pH value is adjusted into 6 in the supernatant layer for obtaining to 2) step, place 60 points
It is placed in a centrifuge after clock and is centrifuged 20 minutes in 2000r/min, gained precipitation and 2) step after centrifugate is filtered
The lower sediment layer mixing for obtaining, obtains mixed precipitation；
4) drinking water of 20 times of amounts are added to obtain constant volume liquid in the mixed precipitation for obtaining to 3) step, to constant volume liquid
After its pH value is adjusted to 8 by middle addition NaOH, 5% alkali protease is added thereto to, is placed in 40 DEG C
Environment in digest 5 hours, obtain digest suspension；
5) the enzymolysis suspension for obtaining 4) step is placed in a centrifuge and is centrifuged 20 minutes in 2000r/min, from
It is divided into two-layer after the heart, upper strata is supernatant layer, and lower floor is the beds of precipitation, takes out the ring that supernatant liquor is placed on 40 DEG C
Dried 24 hours in border, moisture obtains meal after being evaporated, and it is many that the meal obtains final product fructus cannabis of the present invention after crushed
Gly-His-Lys.
Embodiment 3
Raw material：Fructus cannabis
Preparation method is as follows：
1) fructus cannabis raw material is weighed, the drinking water for adding temperature to be 90 DEG C is made into the suspension that solid-liquid ratio is 1: 6,
Mashing, suspension obtains filtered fluid successively with 40 mesh and the screen filtration of 200 mesh after crushing；
2) filtered fluid that 1) step is obtained is placed in a centrifuge and is centrifuged 5 minutes in 4000r/min, divided after centrifugation
It is three layers, upper strata is oil layer, the oil layer is toppled over into removal, middle level is supernatant layer, and lower floor is the beds of precipitation,
Supernatant layer and the beds of precipitation retain standby；
3) add 37% hydrochloric acid that its pH value is adjusted into 3 in the supernatant layer for obtaining to 2) step, place 10 points
It is placed in a centrifuge after clock and is centrifuged 5 minutes in 5000r/min, gained precipitation and 2) step after centrifugate is filtered
The lower sediment layer mixing for obtaining, obtains mixed precipitation；
4) drinking water of 5 times of amounts are added to obtain constant volume liquid in the mixed precipitation for obtaining to 3) step, to constant volume liquid
After its pH value is adjusted to 12 by middle addition NaOH, 0.05% alkali protease is added thereto to, be placed in
Digested 1 hour in 80 DEG C of environment, obtain digesting suspension；
5) the enzymolysis suspension for obtaining 4) step is placed in a centrifuge and is centrifuged 5 minutes in 4000r/min, centrifugation
After be divided into two-layer, upper strata is supernatant layer, and lower floor is the beds of precipitation, takes out supernatant liquor and is placed on 80 DEG C of environment
Middle drying 3 hours, moisture obtains meal after being evaporated, and the meal obtains final product fructus cannabis polypeptide of the present invention after crushed
Powder.
Embodiment 4
Raw material：Fructus cannabis, flavoring apple essence
Preparation method is as follows：
1) weigh fructus cannabis raw material, the drinking water for adding temperature to be 70 DEG C, be made into solid-liquid ratio for 1: 10 it is outstanding
Liquid, mashing, suspension obtains filtered fluid successively with 100 mesh and the screen filtration of 300 mesh after crushing；
2) filtered fluid that 1) step is obtained is placed in a centrifuge and is centrifuged 10 minutes in 3000r/min, after centrifugation
It is divided into three layers, upper strata is oil layer, the oil layer is toppled over into removal, middle level is supernatant layer, and lower floor is precipitation
Layer, supernatant layer and the beds of precipitation retain standby；
3) add 37% hydrochloric acid that its pH value is adjusted into 4.5 in the supernatant layer for obtaining to 2) step, place 30
Minute after be placed in a centrifuge in 4000r/min be centrifuged 10 minutes, after centrifugate is filtered gained precipitation with 2)
The lower sediment layer mixing that step is obtained, obtains mixed precipitation；
4) drinking water of 10 times of amounts are added to obtain constant volume liquid in the mixed precipitation for obtaining to 3) step, to constant volume liquid
After its pH value is adjusted to 9 by middle addition NaOH, 1% alkali protease is added thereto to, is placed in 60 DEG C
Environment in digest 2 hours, obtain digest suspension；
5) the enzymolysis suspension for obtaining 4) step is placed in a centrifuge and is centrifuged 10 minutes in 3000r/min, from
It is divided into two-layer after the heart, upper strata is supernatant layer, and lower floor is the beds of precipitation, takes out the ring that supernatant liquor is placed on 48 DEG C
Dried 12 hours in border, moisture obtains meal after being evaporated, and the meal obtains final product fructus cannabis polypeptide powder after crushed；
6) flavoring apple essence of the fructus cannabis polypeptide powder with 0.5% for obtaining 5) step mixes, and is obtained final product after crushing homogeneous
Fructus cannabis polypeptide powder containing flavoring apple essence.
Embodiment 5
Raw material：Fructus cannabis, flavoring apple essence
Preparation method is as follows：
1) weigh fructus cannabis raw material, the drinking water for adding temperature to be 70 DEG C, be made into solid-liquid ratio for 1: 10 it is outstanding
Liquid, mashing, suspension obtains filtered fluid successively with 100 mesh and the screen filtration of 300 mesh after crushing；
2) filtered fluid that 1) step is obtained is placed in a centrifuge and is centrifuged 10 minutes in 3000r/min, after centrifugation
It is divided into three layers, upper strata is oil layer, the oil layer is toppled over into removal, middle level is supernatant layer, and lower floor is precipitation
Layer, supernatant layer and the beds of precipitation retain standby；
3) add 37% hydrochloric acid that its pH value is adjusted into 4.5 in the supernatant layer for obtaining to 2) step, place 30
Minute after be placed in a centrifuge in 4000r/min be centrifuged 10 minutes, after centrifugate is filtered gained precipitation with 2)
The lower sediment layer mixing that step is obtained, obtains mixed precipitation；
4) drinking water of 10 times of amounts are added to obtain constant volume liquid in the mixed precipitation for obtaining to 3) step, to constant volume liquid
After its pH value is adjusted to 9 by middle addition NaOH, 1% alkali protease is added thereto to, is placed in 60 DEG C
Environment in digest 2 hours, obtain digest suspension；
5) the enzymolysis suspension for obtaining 4) step is placed in a centrifuge and is centrifuged 10 minutes in 3000r/min, from
It is divided into two-layer after the heart, upper strata is supernatant layer, and lower floor is the beds of precipitation, takes out the ring that supernatant liquor is placed on 48 DEG C
Dried 12 hours in border, moisture obtains meal after being evaporated, and the meal obtains final product fructus cannabis polypeptide powder after crushed；
6) flavoring apple essence of the fructus cannabis polypeptide powder with 0.1% for obtaining 5) step mixes, and is obtained final product after crushing homogeneous
Fructus cannabis polypeptide powder containing flavoring apple essence.
Embodiment 6
Raw material：Fructus cannabis, walnut essence
Preparation method is as follows：
1) weigh fructus cannabis raw material, the drinking water for adding temperature to be 70 DEG C, be made into solid-liquid ratio for 1: 10 it is outstanding
Liquid, mashing, suspension obtains filtered fluid successively with 100 mesh and the screen filtration of 300 mesh after crushing；
2) filtered fluid that 1) step is obtained is placed in a centrifuge and is centrifuged 10 minutes in 3000r/min, after centrifugation
It is divided into three layers, upper strata is oil layer, the oil layer is toppled over into removal, middle level is supernatant layer, and lower floor is precipitation
Layer, supernatant layer and the beds of precipitation retain standby；
3) add 37% hydrochloric acid that its pH value is adjusted into 4.5 in the supernatant layer for obtaining to 2) step, place 30
Minute after be placed in a centrifuge in 4000r/min be centrifuged 10 minutes, after centrifugate is filtered gained precipitation with 2)
The lower sediment layer mixing that step is obtained, obtains mixed precipitation；
4) drinking water of 10 times of amounts are added to obtain constant volume liquid in the mixed precipitation for obtaining to 3) step, to constant volume liquid
After its pH value is adjusted to 9 by middle addition NaOH, 1% alkali protease is added thereto to, is placed in 60 DEG C
Environment in digest 2 hours, obtain digest suspension；
5) the enzymolysis suspension for obtaining 4) step is placed in a centrifuge and is centrifuged 10 minutes in 3000r/min, from
It is divided into two-layer after the heart, upper strata is supernatant layer, and lower floor is the beds of precipitation, takes out the ring that supernatant liquor is placed on 48 DEG C
Dried 12 hours in border, moisture obtains meal after being evaporated, and the meal obtains final product fructus cannabis polypeptide powder after crushed；
6) walnut essence of the fructus cannabis polypeptide powder with 0.8% for obtaining 5) step mixes, and is obtained final product after crushing homogeneous
Fructus cannabis polypeptide powder containing walnut essence.
Claims (5)
1. a kind of fructus cannabis polypeptide powder, it is characterised in that：The raw material of the fructus cannabis polypeptide powder is made for fructus cannabis,
The fructus cannabis polypeptide powder is prepared from accordance with the following methods：
1) fructus cannabis raw material is weighed, the drinking water for adding temperature to be 40-90 DEG C, it is 1: 6-1: 20 to be made into solid-liquid ratio
Suspension, mashing, suspension was obtained successively with the screen filtration of 40-200 mesh and 200-400 mesh after crushing
Filtrate；
2) filtered fluid that 1) step is obtained is placed in a centrifuge and is centrifuged 5-20 minutes in 2000-4000r/min,
It is divided into three layers after centrifugation, upper strata is oil layer, the oil layer is toppled over into removal, middle level is supernatant layer, lower floor
It is the beds of precipitation, supernatant layer and the beds of precipitation retain standby；
3) add 37% hydrochloric acid that its pH value is adjusted into 3-6 in the supernatant layer for obtaining to 2) step, place 10-60
It is placed in a centrifuge after minute and is centrifuged 5-20 minutes in 2000-5000r/min, gained is heavy after centrifugate is filtered
The lower sediment layer that shallow lake obtains with 2) step mixes, and obtains mixed precipitation；
4) the 5-20 times of drinking water measured is added to obtain constant volume liquid in the mixed precipitation for obtaining to 3) step, to constant volume
After adding NaOH that its pH value is adjusted into 8-12 in liquid, the basic protein of 0.05%-5% is added thereto to
Enzyme, is placed in 40-80 DEG C of environment and digests 1-5 hours, obtains digesting suspension；
5) the enzymolysis suspension for obtaining 4) step is placed in a centrifuge in 5-20 points of 2000-4000r/min centrifugations
Clock, is divided into two-layer after centrifugation, upper strata is supernatant layer, and lower floor is the beds of precipitation, takes out supernatant liquor and is placed on
Dried in 40-80 DEG C of environment 3-24 hours, moisture obtains meal after being evaporated, the meal obtains final product this after crushed
Invention fructus cannabis polypeptide powder.
2. fructus cannabis polypeptide powder as claimed in claim 1, it is characterised in that：It is made the raw material of fructus cannabis polypeptide powder
In also include flavoring essence, the fructus cannabis polypeptide powder containing flavoring essence is prepared from accordance with the following methods：
1) fructus cannabis raw material is weighed, the drinking water for adding temperature to be 40-90 DEG C, it is 1: 6-1: 20 to be made into solid-liquid ratio
Suspension, mashing, suspension was obtained successively with the screen filtration of 40-200 mesh and 200-400 mesh after crushing
Filtrate；
2) filtered fluid that 1) step is obtained is placed in a centrifuge and is centrifuged 5-20 minutes in 2000-4000r/min,
It is divided into three layers after centrifugation, upper strata is oil layer, the oil layer is toppled over into removal, middle level is supernatant layer, lower floor
It is the beds of precipitation, supernatant layer and the beds of precipitation retain standby；
3) add 37% hydrochloric acid that its pH value is adjusted into 3-6 in the supernatant layer for obtaining to 2) step, place 10-60
It is placed in a centrifuge after minute and is centrifuged 5-20 minutes in 2000-5000r/min, gained is heavy after centrifugate is filtered
The lower sediment layer that shallow lake obtains with 2) step mixes, and obtains mixed precipitation；
4) the 5-20 times of drinking water measured is added to obtain constant volume liquid in the mixed precipitation for obtaining to 3) step, to constant volume
After adding NaOH that its pH value is adjusted into 8-12 in liquid, the basic protein of 0.05%-5% is added thereto to
Enzyme, is placed in 40-80 DEG C of environment and digests 1-5 hours, obtains digesting suspension；
5) the enzymolysis suspension for obtaining 4) step is placed in a centrifuge in 5-20 points of 2000-4000r/min centrifugations
Clock, is divided into two-layer after centrifugation, upper strata is supernatant layer, and lower floor is the beds of precipitation, takes out supernatant liquor and is placed on
Dried in 40-80 DEG C of environment 3-24 hours, moisture obtains meal after being evaporated, the meal obtains final product fire after crushed
Edestan polypeptide powder；
6) the fructus cannabis polypeptide powder for obtaining 5) step mixes with the flavoring essence of 0.1%-0.8%, crushes homogeneous
The fructus cannabis polypeptide powder containing flavoring essence is obtained final product afterwards.
3. fructus cannabis polypeptide powder as claimed in claim 2, it is characterised in that：The flavoring essence is flavoring apple essence
Or walnut essence.
4. fructus cannabis polypeptide powder as claimed in claim 1, it is characterised in that the fructus cannabis polypeptide powder is according to following
Method is prepared from：
1) weigh fructus cannabis raw material, the drinking water for adding temperature to be 70 DEG C, be made into solid-liquid ratio for 1: 10 it is outstanding
Liquid, mashing, suspension obtains filtered fluid successively with 100 mesh and the screen filtration of 300 mesh after crushing；
2) filtered fluid that 1) step is obtained is placed in a centrifuge and is centrifuged 10 minutes in 3000r/min, after centrifugation
It is divided into three layers, upper strata is oil layer, the oil layer is toppled over into removal, middle level is supernatant layer, and lower floor is precipitation
Layer, supernatant layer and the beds of precipitation retain standby；
3) add 37% hydrochloric acid that its pH value is adjusted into 4.5 in the supernatant layer for obtaining to 2) step, place 30
Minute after be placed in a centrifuge in 4000r/min be centrifuged 10 minutes, after centrifugate is filtered gained precipitation with 2)
The lower sediment layer mixing that step is obtained, obtains mixed precipitation；
4) drinking water of 10 times of amounts are added to obtain constant volume liquid in the mixed precipitation for obtaining to 3) step, to constant volume liquid
After its pH value is adjusted to 9 by middle addition NaOH, 1% alkali protease is added thereto to, is placed in 60 DEG C
Environment in digest 2 hours, obtain digest suspension；
5) the enzymolysis suspension for obtaining 4) step is placed in a centrifuge and is centrifuged 10 minutes in 3000r/min, from
It is divided into two-layer after the heart, upper strata is supernatant layer, and lower floor is the beds of precipitation, takes out the ring that supernatant liquor is placed on 48 DEG C
Dried 12 hours in border, moisture obtains meal after being evaporated, and it is many that the meal obtains final product fructus cannabis of the present invention after crushed
Gly-His-Lys.
5. fructus cannabis polypeptide powder as claimed in claim 2, it is characterised in that the fructus cannabis containing flavoring essence is more
Gly-His-Lys are prepared from accordance with the following methods：
1) weigh fructus cannabis raw material, the drinking water for adding temperature to be 70 DEG C, be made into solid-liquid ratio for 1: 10 it is outstanding
Liquid, mashing, suspension obtains filtered fluid successively with 100 mesh and the screen filtration of 300 mesh after crushing；
2) filtered fluid that 1) step is obtained is placed in a centrifuge and is centrifuged 10 minutes in 3000r/min, after centrifugation
It is divided into three layers, upper strata is oil layer, the oil layer is toppled over into removal, middle level is supernatant layer, and lower floor is precipitation
Layer, supernatant layer and the beds of precipitation retain standby；
3) add 37% hydrochloric acid that its pH value is adjusted into 4.5 in the supernatant layer for obtaining to 2) step, place 30
Minute after be placed in a centrifuge in 4000r/min be centrifuged 10 minutes, after centrifugate is filtered gained precipitation with 2)
The lower sediment layer mixing that step is obtained, obtains mixed precipitation；
4) drinking water of 10 times of amounts are added to obtain constant volume liquid in the mixed precipitation for obtaining to 3) step, to constant volume liquid
After its pH value is adjusted to 9 by middle addition NaOH, 1% alkali protease is added thereto to, is placed in 60 DEG C
Environment in digest 2 hours, obtain digest suspension；
5) the enzymolysis suspension for obtaining 4) step is placed in a centrifuge and is centrifuged 10 minutes in 3000r/min, from
It is divided into two-layer after the heart, upper strata is supernatant layer, and lower floor is the beds of precipitation, takes out the ring that supernatant liquor is placed on 48 DEG C
Dried 12 hours in border, moisture obtains meal after being evaporated, and the meal obtains final product fructus cannabis polypeptide powder after crushed；
6) flavoring essence of the fructus cannabis polypeptide powder with 0.5% for obtaining 5) step mixes, and is obtained final product after crushing homogeneous
Fructus cannabis polypeptide powder containing flavoring essence.
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