CN103740796A - Method for extracting polypeptides from skimmed protein milk supernatant - Google Patents
Method for extracting polypeptides from skimmed protein milk supernatant Download PDFInfo
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- CN103740796A CN103740796A CN201410015528.6A CN201410015528A CN103740796A CN 103740796 A CN103740796 A CN 103740796A CN 201410015528 A CN201410015528 A CN 201410015528A CN 103740796 A CN103740796 A CN 103740796A
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Abstract
The invention discloses a method for extracting polypeptides from skimmed protein milk supernatant. The method comprises the following steps: (1) thermally grinding, pulping and centrifuging lipid-containing plants so as to obtain light phase liquid serving as vegetable fat, and obtain heavy phase liquid serving as skimmed protein milk; (2) regulating the pH to be 4.0-5.0, and precipitating under the isoelectric point of the skimmed protein milk so as to obtain skimmed milk supernatant and protein concentrated pulp; (3) collecting the skimmed milk supernatant, regulating the pH of the skimmed milk supernatant to be 8.2-8.9, adding trypsin for performing enzymolysis for 3.5-6 hours so as to obtain an enzymolysis solution; (4) performing enzyme deactivation on the enzymolysis solution to obtain an enzyme-free solution; (5) adding activated carbon into the enzyme-free solution, removing the activated carbon by utilizing a centrifugal machine so as to obtain the supernatant; and (6) concentrating the supernatant, performing spray drying so as to obtain the polypeptide powder. According to the method, a step of extracting the proteins is reduced, and the process flow is short, the operation is simple, and the processing cost is low and industrial production is facilitated.
Description
Technical field
The present invention relates to the preparation method of a peptide species, particularly from contain fat plant apolipoprotein breast supernatant liquor, extract the method for polypeptide.
Background technology
Polypeptide is that a-amino acid links together with peptide chain and the compound that forms, and it is also the intermediate product of proteolysis.Two amino acid molecular dehydrating condensations can form dipeptides, and a plurality of amino acid molecular dehydrating condensations can form tripeptides, tetrapeptide, pentapeptide etc.Conventionally the compound being formed by 10～100 amino acid molecular dehydrating condensations is polypeptide, and its molecular weight, lower than 10,000 dalton, can see through semi-permeable membranes, by trichoroacetic acid(TCA) and ammonium sulfate, is not precipitated.
The absorption of human body to protein is mainly to carry out the degradation of proteins into the form of low peptide by the effect of proteolytic enzyme.Experiment shows that peptide compares amino acid and can be absorbed more easily, faster by mucous membrane of small intestine.Protein after enzymic hydrolysis, can obtain the mixture of polypeptide, and the solvability of these mixtures under acidic conditions improves greatly, and has the good characteristic that concentration high viscosity is low.Although the research of vegetable-protein bioactive peptide has been reached to certain level both at home and abroad, and related products has been applied to health care, but be mainly soybean active peptide product, just at the early-stage for the bioactive peptide research of other plant, related products is very few, cannot meet foodstuffs industry demand.
The extraction of domestic polypeptide is mainly to isolate protein from feed grouts, then through enzymolysis, extract polypeptide, no matter be traditional milling process or lixiviation process, in the dregs of rice that make all can there is sex change to a certain degree in protein, this has brought a very large difficult problem to the deep processing of protein, cause the extraction yield of polypeptide low, protein utilization is not high.There are a lot of defects in the dregs of rice that traditional technology method makes, in milling process, the residual oil content in the dregs of rice is high, caused very large difficulty to the separation and purification of protein, and milling process makes the dregs of rice through high temperature, and protein denaturation is serious, is more unfavorable for the extraction of protein; The dregs of rice residual organic solvent that lixiviation process makes, directly affects the quality of protein separation, and the dregs of rice have become waste liquid after proteins extraction, cannot carry out processing and utilization again, cause prepared using insufficient, waste resource.
Li Xionghui, crossing new victory waits and in research > > mono-literary composition of the < < soybean polypeptide extraction process of delivering in 09 phase < < Food science > > for 1999, soybean is first carried out to low temperature desolventizing and obtain dregs of beans, through water extraction process, tentatively obtain protein again, use afterwards the heavy way of acid to carry out protein purification, finally use two enzymes to be hydrolyzed and to obtain polypeptide protein.This method raw material is single, extracts protein process complexity, high cost from soybean.Xu Huaide, Chen Jinhai etc. disclose a kind of making method of walnut polypeptide powder in 200710017281.1, the method has not only retained the multiple nutritional components of walnut, and the content of walnut polypeptide powder polypeptide has reached 60-80%, first this invention process is that walnut dregs is pulverized, and then adopts supersonic method lixiviate protein.To protein after vacuum-drying, use papoid to carry out enzymolysis, through the enzyme that goes out, centrifugal, get supernatant liquor dialysis, through concentrated, after vacuum-drying, can obtain polypeptide.It is large that this method is extracted protein difficulty from the dregs of rice, and need further to purify, and energy consumption is large.
The raw material that domestic enzyme process polypeptide maturation process is used is that protein content is 90% soybean protein isolate.Soybean protein isolate can produce the serious problems such as environmental pollution in process of production, does not meet China's strategy of sustainable development, will progressively be eliminated.All the other enzymolysis polypeptides utilize feedstuff protein as raw material mostly, by alkali extraction and acid precipitation, extract after protein, and recycling proteolytic enzyme comes enzymolysis to make polypeptide powder.This method has the problems such as leaching process is complicated, and waste is too many, protein extraction difficulty.
Summary of the invention
In order to solve the problems of the technologies described above, the invention provides a kind of method of extracting polypeptide from apolipoprotein breast supernatant liquor, the method has reduced the extraction step of protein, and technical process is short, simple to operate, is suitable for suitability for industrialized production.
For this reason, technical scheme of the present invention is as follows:
A method of extracting polypeptide from apolipoprotein breast supernatant liquor, comprises the steps:
1) will through whizzer, process containing fat plant heat grinding slurry, obtaining light phase liquid is Vegetable oil lipoprotein, and heavy-phase liquid is apolipoprotein breast;
2) regulate described apolipoprotein breast pH=4.0～5.0, then precipitate under the iso-electric point of described apolipoprotein breast, obtain skimming milk supernatant liquor and albumen underflow;
3) collect described skimming milk supernatant liquor, and regulate its pH=8.2～8.9, then add wherein the trypsinase of described skimming milk supernatant liquor quality 2～4%, enzymolysis 3.5～6h under 40 ℃, agitation condition, obtains enzymolysis solution;
4) described enzymolysis solution is heated to 4～8min under 85～95 ℃ of conditions, the enzyme that goes out obtains without enzyme liquid;
5) to described without adding the gac that accounts for its quality 4～6% in enzyme liquid, stir 15～40min debitterizing and decoloring; Then remove gac wherein, obtain supernatant liquor;
6) by described supernatant concentration to solids content, be that after 30～40wt%, to carry out spray dried dry, obtain polypeptide powder.
Described is walnut kernel, Semen arachidis hypogaeae, corn, soybean, sunflower seeds, linseed oil, vegetable seed, sesame or almond containing fat plant.
The Ruzhong of apolipoprotein described in step 1) protein content is 21～46wt%.
Deposition rate while obtaining skimming milk supernatant liquor and albumen underflow step 2) is 73～88%.
In step 2) in by HCl, regulate pH value.
In step 3), by NaOH, regulate pH value.
What step 5) was removed gac use is vibration discharging centrifugal machine, helical-conveyer centrifugal.
In step 6), the concentration method of supernatant liquor is for to utilize falling-film evaporator by described supernatant liquor, at vacuum tightness 850～890mbar, at 48～52 ℃ of temperature, carries out vacuum concentration.
The method has reduced the extraction step of protein, and technical process is short, simple to operate, and tooling cost is low, is suitable for suitability for industrialized production.And the polypeptide making can be widely used in, in protective foods, having very high nutritive value.
Embodiment
Below in conjunction with embodiment, preparation method of the present invention is described in detail.
Embodiment 1
From walnut kernel skimming milk supernatant liquor, extract the method for polypeptide
1) walnut kernel is soaked after 2 hours in the aqueous sodium hydroxide solution of 2wt%, with clear water, rinse well, then use the peracetic acid disinfectant of 0.35wt%, then use clear water spray irrigation clean;
2) in the walnut kernel after processing to step 1), add the water of its 5 times of quality, at about 50 ℃ use grinding-wheel grinder pulp grinder heat grinding slurries, isolate slag crust and discolor; Again gained slurries being milled to fineness with colloidal mill is 2～4 μ m; Then colloidal mill is ground to rear slurry and insert disk centrifugal separator 45 ℃ of left and right, centrifugation under rotating speed 7310r/min condition, obtaining light phase liquid is Vegetable oil lipoprotein; Heavy-phase liquid is apolipoprotein breast; Slag-drip opening obtains having the slag crust of high added value;
3) the apolipoprotein breast obtaining is inserted to paste roller mill, carry out defibrination 10 times, utilizing Kjeldahl determination to record total nitrogen is 3.5%, and the content that is converted into protein is 22%;
4) then to step 3), obtain adding in solution HCl, regulate its pH=4.9, under the iso-electric point of described apolipoprotein breast, precipitate again, when deposition rate is 80%, obtain skimming milk supernatant liquor and albumen underflow, by described albumen underflow being concentrated, spray to be dried after both separation, obtain protein powder;
5) get described skimming milk supernatant liquor, with Kjeldahl determination, recording total nitrogen is 0.6%, and being converted into protein content is 3.8%, the NaOH that adds wherein 1mol/L, regulates its pH=8.5, adds the trypsinase that accounts for its quality 4%, under 40 ℃, agitation condition, enzymolysis is 3.5 hours, obtains enzymolysis solution;
6) described enzymolysis solution is heated 6 minutes in 90 ℃ of waters bath with thermostatic control, the enzyme that goes out, obtains without enzyme liquid;
7) by described, without enzyme liquid, be cooled to rapidly 30 ℃ again, add the gac that accounts for its quality 4%, continue to stir 25 minutes debitterizing and decoloring; Inserted again helical-conveyer centrifugal, centrifugal treating under 4000r/min condition, the supernatant liquor of the gac that is removed;
8) described supernatant liquor is utilized to falling-film evaporator, under the condition of vacuum tightness 850mbar, temperature 50 C, carry out vacuum concentration, while being about 35wt% to solids content, utilize drying machine with centrifugal spray, in inlet temperature, be under 160 ℃, the pressure condition that is 0.40MPa, the dry polypeptide powder that makes of spraying.
Embodiment 2
From Semen arachidis hypogaeae skimming milk supernatant liquor, extract the method for polypeptide
1) Semen arachidis hypogaeae is soaked after 1.5 hours in the aqueous sodium hydroxide solution of 1wt%, with clear water, rinse well, then use the peracetic acid disinfectant of 0.35wt%, then use clear water spray irrigation clean;
2) in the Semen arachidis hypogaeae after processing to step 1), add the water of its 4 times of quality, at about 50 ℃ use grinding-wheel grinder pulp grinder heat grinding slurries, isolate slag crust and discolor; Again gained slurries being milled to fineness with colloidal mill is 2～4 μ m; Then colloidal mill is ground to rear slurry and insert disk centrifugal separator 45 ℃ of left and right, centrifugation under rotating speed 7310r/min condition, obtaining light phase liquid is Vegetable oil lipoprotein; Heavy-phase liquid is apolipoprotein breast; Slag-drip opening obtains having the slag crust of high added value;
3) the apolipoprotein breast obtaining is inserted to paste roller mill, carry out defibrination 8 times, utilizing Kjeldahl determination to record total nitrogen is 5.68%, and the content that is converted into protein is 31%;
4) then to step 3), obtain adding in solution HCl, regulate its pH=4.5, under the iso-electric point of described apolipoprotein breast, precipitate again, when deposition rate is 73%, obtain skimming milk supernatant liquor and albumen underflow, by described albumen underflow being concentrated, spray to be dried after both separation, obtain protein powder;
5) get described skimming milk supernatant liquor, with Kjeldahl determination, recording total nitrogen is 0.75%, and being converted into protein content is 4.1%, the NaOH that adds wherein 1mol/L, regulates its pH=8.5, adds the trypsinase that accounts for its quality 3%, under 40 ℃, agitation condition, enzymolysis is 4 hours, obtains enzymolysis solution;
6) described enzymolysis solution is heated 7 minutes under 90 ℃ of conditions, the enzyme that goes out, obtains without enzyme liquid;
7) by described, without enzyme liquid, be cooled to rapidly 30 ℃ again, add the gac that accounts for its quality 5%, continue to stir 30 minutes debitterizing and decoloring; Inserted again helical-conveyer centrifugal, centrifugal treating under 4200r/min condition, the supernatant liquor of the gac that is removed;
8) described supernatant liquor is utilized to falling-film evaporator, under the condition of 49 ℃ of vacuum tightness 880mbar, temperature, carry out vacuum concentration, while being about 35% to solids content, utilize drying machine with centrifugal spray, in inlet temperature, be under 160 ℃, the pressure condition that is 0.40MPa, the dry polypeptide powder that makes of spraying.
Embodiment 3
From soybean skimming milk supernatant liquor, extract the method for polypeptide
1) soybean is soaked after 2 hours in the aqueous sodium hydroxide solution of 2wt%, with clear water, rinsed well, then use the peracetic acid disinfectant of 0.35wt%, then use clear water spray irrigation clean;
2) in the soybean after processing to step 1), add the water of its 3 times of quality, at about 50 ℃ use grinding-wheel grinder pulp grinder heat grinding slurries, isolate slag crust and discolor; Again gained slurries being milled to fineness with colloidal mill is 2～4 μ m; Then colloidal mill is ground to rear slurry and insert whizzer 45 ℃ of left and right, centrifugation under rotating speed 7310r/min condition, obtaining light phase liquid is Vegetable oil lipoprotein; Heavy-phase liquid is apolipoprotein breast; Slag-drip opening obtains having the slag crust of high added value;
3) the apolipoprotein breast obtaining is inserted to paste roller mill, carry out defibrination 6 times, utilizing Kjeldahl determination to record total nitrogen is 7.2%, and the content that is converted into protein is 41%;
4) then to step 3), obtain adding in solution HCl, regulate its pH=4.5, under the iso-electric point of described apolipoprotein breast, precipitate again, when deposition rate is 78%, obtain skimming milk supernatant liquor and albumen underflow, by described albumen underflow being concentrated, spray to be dried after both separation, obtain protein powder;
5) get described skimming milk supernatant liquor, with Kjeldahl determination, recording total nitrogen is 0.79%, and being converted into protein content is 4.5%, the NaOH that adds wherein 1mol/L, regulates its pH=8.5, adds the trypsinase that accounts for its quality 3%, under 40 ℃, agitation condition, enzymolysis is 5 hours, obtains enzymolysis solution;
6) described enzymolysis solution is heated 4 minutes under 90 ℃ of conditions, the enzyme that goes out, obtains without enzyme liquid;
7) by described, without enzyme liquid, be cooled to rapidly 30 ℃ again, add the gac that accounts for its quality 5%, continue to stir 30 minutes debitterizing and decoloring; Inserted again vibration discharging centrifugal machine, centrifugal treating under 4200r/min condition, the supernatant liquor of the gac that is removed;
8) described supernatant liquor is utilized to falling-film evaporator, at vacuum tightness 890mbar, in the situation that temperature is 48 ℃, carry out vacuum concentration, while being about 35% to solids content, utilizing drying machine with centrifugal spray, is under 160 ℃, the pressure condition that is 0.40MPa in inlet temperature, the dry polypeptide powder that makes of spraying.
In the present invention, the measuring method of protein content is: utilize Kjeldahl determination to record total nitrogen content, be then converted into protein content.
Determining content of peptides:
It is the trichloroacetic acid solution of 15wt% that the polypeptide powder making in embodiment 1～3 is added to concentration, being stirred well to high molecular weight protein precipitates completely, and micromolecule polypeptide is retained in solution, remove the supernatant liquor that precipitation in solution obtains being dissolved with polypeptide, according to the total nitrogen content of the test determines supernatant liquor of GB/T5009.5-2010, then extrapolate the content of polypeptide.
That is: embodiment 1,2,3 makes the content of polypeptide in polypeptide powder and is respectively 74%, 75% and 72%.
The polypeptide preparation yolk pie of utilizing the inventive method to make:
The method of the polypeptide preparation polypeptide group that embodiment 1,2,3 makes, comprises the steps:
1) heart fillings is sent in preparation: by polypeptide, functional oligose, egg is put into basin and beaten thin out to yolk color or turn white with egg-whisk, and oligose all dissolves; The aqueous solution that adds polypeptide powder, stirs, and adds weak strength flour, after stirring, again add the aqueous solution of polypeptide powder, stir and obtain mixed pulp, described mixed pulp is endured slowly with little fire, with rubber squeegee, be stirred to mixed pulp simultaneously and become pasty state, from fire, add rapidly butter, stir, add appropriate vanilla, milk flavour and emulsifying agent, obtain sending heart fillings, by described, send heart fillings to cool to be placed on freezer compartment of refrigerator to be preserved.
2) preparing cake sticks with paste: by shell egg, functional oligose put into basin with egg-whisk beat to egg liquid, expand turn white, liquid is dense thick; Add weak strength flour, Mierocrystalline cellulose, pectinose, full grain flour, sorbyl alcohol, malt sugar, baking powder, citric acid, salt and shortening to stir, then add weak strength flour to stir to obtain cake and stick with paste;
3) prepare polypeptide group: described cake is stuck with paste and poured in mould to 1/4 full, put into the roasting 3min of baking box that is preheated to 180 ℃, in mould, put into the filling heart suitable size, that freeze again, secondly cake is stuck with paste pour in mould to 9 one-tenth full, put into baking box, at 180 ℃, bake and can obtain containing polypeptide group.
Suggestion massfraction raw materials used in aforesaid method is as follows: functional oligose 16%, weak strength flour 26%, egg 4%, full grain flour 10%, polypeptide powder 15%, water 8%, pectinose 1%, Mierocrystalline cellulose 2.7, emulsifying agent 4%, shortening 8%, sorbyl alcohol 1%, malt sugar 3%, baking powder 0.7%, salt 0.4%, vanilla 0.1%, milk flavour 0.07%, citric acid 0.03%.
In the group that the method makes, contain polypeptide, had the several functions that polypeptide is easy to, decreasing cholesterol fast, anti-oxidant, hypotensive by intestinal absorption, absorption rate, antifatigue concurrently.And the pectinose containing in group and Mierocrystalline cellulose can suppress human body to the absorption of sucrose, promotion intestines peristalsis, be suitable for designed for old people.
Claims (8)
1. from apolipoprotein breast supernatant liquor, extract a method for polypeptide, it is characterized in that: comprise the steps:
1) will through whizzer, process containing fat plant heat grinding slurry, obtaining light phase liquid is Vegetable oil lipoprotein, and heavy-phase liquid is apolipoprotein breast;
2) regulate described apolipoprotein breast pH=4.0～5.0, then precipitate under the iso-electric point of described apolipoprotein breast, obtain skimming milk supernatant liquor and albumen underflow;
3) collect described skimming milk supernatant liquor, and regulate its pH=8.2～8.9, then add wherein the trypsinase of described skimming milk supernatant liquor quality 2～4%, enzymolysis 3.5～6h under 40 ℃, agitation condition, obtains enzymolysis solution;
4) described enzymolysis solution is heated to 4～8min under 85～95 ℃ of conditions, the enzyme that goes out obtains without enzyme liquid;
5) to described without adding the gac that accounts for its quality 4～6% in enzyme liquid, stir 15～40min debitterizing and decoloring; Then remove gac wherein, obtain supernatant liquor;
6) by described supernatant concentration to solids content, be that after 30～40wt%, to carry out spray dried dry, obtain polypeptide powder.
2. the method for claim 1, is characterized in that: described is walnut kernel, Semen arachidis hypogaeae, corn, soybean, sunflower seeds, linseed oil, vegetable seed, sesame or almond containing fat plant.
3. the method for claim 1, is characterized in that: the Ruzhong of apolipoprotein described in step 1) protein content is 21～46wt%.
4. the method for claim 1, is characterized in that: step 2) in deposition rate while obtaining skimming milk supernatant liquor and albumen underflow be 73～88%.
5. the method for claim 1, is characterized in that: in step 2) in by HCl, regulate pH value.
6. the method for claim 1, is characterized in that: in step 3), by NaOH, regulate pH value.
7. the method for claim 1, is characterized in that: what step 5) was removed gac use is vibration discharging centrifugal machine, helical-conveyer centrifugal.
8. the method for claim 1, is characterized in that: in step 6), the concentration method of supernatant liquor, for described supernatant liquor is utilized to falling-film evaporator, at vacuum tightness 850～890mbar, carries out vacuum concentration at 48～52 ℃ of temperature.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105622739A (en) * | 2016-02-15 | 2016-06-01 | 天津替代医学科技股份有限公司 | Method for extracting ubiquitin and like ubiquitin from protein milk |
| CN106889298A (en) * | 2015-12-21 | 2017-06-27 | 北京华昱安然医药科技有限公司 | A kind of fructus cannabis polypeptide powder and preparation method thereof |
| CN108179164A (en) * | 2018-02-08 | 2018-06-19 | 金华市铁骑士生物科技有限公司 | The method that polypeptide is extracted from degreasing protein breast supernatant |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004085664A2 (en) * | 2003-03-25 | 2004-10-07 | Council Of Scientific And Industrial Research | Process for the preparation of angiotensin converting enzyme (ace) inhibitors and its use |
| CN102505034A (en) * | 2011-12-02 | 2012-06-20 | 安徽燕之坊食品有限公司 | Method for preparing active polypeptide from corn protein powder |
| CN102533918A (en) * | 2012-01-18 | 2012-07-04 | 安徽燕之坊食品有限公司 | Method for preparing rapeseed active polypeptides |
| CN103392904A (en) * | 2013-08-02 | 2013-11-20 | 江苏丘陵地区镇江农业科学研究所 | Method used for extracting black soya bean polypeptide by combining wet ball milling with enzyme method |
-
2014
- 2014-01-14 CN CN201410015528.6A patent/CN103740796B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004085664A2 (en) * | 2003-03-25 | 2004-10-07 | Council Of Scientific And Industrial Research | Process for the preparation of angiotensin converting enzyme (ace) inhibitors and its use |
| CN102505034A (en) * | 2011-12-02 | 2012-06-20 | 安徽燕之坊食品有限公司 | Method for preparing active polypeptide from corn protein powder |
| CN102533918A (en) * | 2012-01-18 | 2012-07-04 | 安徽燕之坊食品有限公司 | Method for preparing rapeseed active polypeptides |
| CN103392904A (en) * | 2013-08-02 | 2013-11-20 | 江苏丘陵地区镇江农业科学研究所 | Method used for extracting black soya bean polypeptide by combining wet ball milling with enzyme method |
Non-Patent Citations (1)
| Title |
|---|
| 李雄辉等: "大豆多肽提取工艺的研究", 《食品科学》, no. 9, 30 September 1999 (1999-09-30), pages 28 - 30 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106889298A (en) * | 2015-12-21 | 2017-06-27 | 北京华昱安然医药科技有限公司 | A kind of fructus cannabis polypeptide powder and preparation method thereof |
| CN105622739A (en) * | 2016-02-15 | 2016-06-01 | 天津替代医学科技股份有限公司 | Method for extracting ubiquitin and like ubiquitin from protein milk |
| CN108179164A (en) * | 2018-02-08 | 2018-06-19 | 金华市铁骑士生物科技有限公司 | The method that polypeptide is extracted from degreasing protein breast supernatant |
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