CN106868041A - Method and application using arabidopsis AtYchF1 gene plant weedings - Google Patents
Method and application using arabidopsis AtYchF1 gene plant weedings Download PDFInfo
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- CN106868041A CN106868041A CN201710148497.5A CN201710148497A CN106868041A CN 106868041 A CN106868041 A CN 106868041A CN 201710148497 A CN201710148497 A CN 201710148497A CN 106868041 A CN106868041 A CN 106868041A
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Abstract
The present invention is provided using the method for arabidopsis AtYchF1 gene plant weedings, is comprised the following steps：Step 1：Cultivate the crop plants of missing AtYchF1 homologous genes；Step 2：The crop plants of the missing AtYchF1 homologous genes cultivated in field planting step 1；Step 3：Apply crop plants tolerance, effective dose herbicide to weeds and crop plants to control weeds in field.Arabidopsis AtYchF1 genes take part in resistance of the plant for herbicide, and the extremely strong (homology of protein sequence conservative due to YchF family members between different plant species in translation skill>90%), therefore, for the mutant of AtYchF1 genes, for the industrial crops new varieties for cultivating new herbicide-resistant provide new possibility, and then reach the purpose for not injuring crops again using the efficient weeding of herbicide.
Description
Technical field
The invention belongs to genetic engineering applied technical field, more particularly to using arabidopsis AtYchF1 gene plant weedings
Method and application.
Background technology
With the continuous growth of world population, the mankind are increasing to the demand of grain and other crops.In order to promote
Grain and the growth of other crops, realize grain and the volume increase of other crops, prevent weeds near crop and grain and
Other crops compete nutritional ingredient, it is often necessary to remove the weeds near grain and other crops, traditional weeding side
Formula is artificial weeding (mowing or uprooting weed), but artificial weeding labour intensity is big, efficiency is low and human cost is high；Using herbicide
(paraquat) can greatly improve weeding ratio, effectively reduce labour intensity and human cost, paraquat (1-1- dimethyl -4-4-
Bipyridine cation salt) it is a kind of quick property killed herbicide, the mechanism of its weeding is, as electron acceptor, to act on plant line
Plastochondria, participates in redox regulatory, produces substantial amounts of active oxygen radical, including super oxide anion free radical (O=) and mistake
Hydrogen oxide (H2O2), the death of plant is ultimately resulted in, because paraquat is to grain and other crops and weeds and non-selectivity,
Meanwhile, grain and other crops are not high enough to the tolerance of paraquat, therefore, when weeds are pressed from both sides with by grain and other crops
It is miscellaneous to grow when together, it is easy to injury using herbicide (paraquat) weeding and even kills grain and other crops.
The content of the invention
It is an object of the invention to overcome drawbacks described above, there is provided using the side of arabidopsis AtYchF1 gene plant weedings
Method and application, new possibility is provided to cultivate herbicide-tolerant crops new varieties.
In order to solve the above-mentioned technical problem, the technical solution adopted by the present invention is：
The present invention is provided using the method for arabidopsis AtYchF1 gene plant weedings, is comprised the following steps：
Step 1：Cultivate the crop plants of missing AtYchF1 homologous genes；
Step 2：The crop plants of the missing AtYchF1 homologous genes cultivated in field planting step 1；
Step 3：Apply crop plants tolerance, effective dose herbicide in field miscellaneous to control to weeds and crop plants
Grass.
The present invention is also provided using the application of arabidopsis AtYchF1 gene plant weedings, and the AtYchF1 genes exist
Numbering in Genebank is AT1G30580, and as shown in sequence 1 in sequence table, the amino acid sequence of its coding is such as its DNA sequence dna
In sequence table shown in sequence 2；
The AtYchF1 genes are related to plant resistant herbicide function, after by AtYchF1 gene delections, mutant
Plant is strengthened for the tolerance of herbicide；
And after by AtYchF1 gene overexpressions, mutant plants are lifted for the susceptibility of herbicide.
The beneficial effects of the present invention are：Because herbicide (paraquat) acts on plant mitochondria, redox is participated in
Regulation and control, produce substantial amounts of active oxygen radical, and inventor is proved by a series of experiments, and arabidopsis AtYchF1 genes are also joined
With the redox regulatory of inside plants, and resistance of the plant for herbicide (paraquat) is take part in translation skill, and by
Extremely strong (the homology of protein sequence conservative in YchF family members between different plant species>90%), therefore, for
The mutant of AtYchF1 genes, for the industrial crops new varieties for cultivating new herbicide-resistant (paraquat) provide new possibility,
And then reach using herbicide (paraquat) removing weeds, but the purpose of crops is not injured；This herbicidal methods is made by improving
Thing plant recycles herbicide to remove plant crop weeds nearby to the tolerance of herbicide, has both maintained the efficient of chemical weed control
Property, injury of the herbicide (paraquat) to crop is effectively prevent again, it is workable, with certain generalization.
Brief description of the drawings
Fig. 1 is that deletion mutant AtYchF1 and wild type Col-2 arabidopsis are grown in containing hundred in the embodiment of the present invention 1
Phenotype on the withered culture medium of grass.
Fig. 2 is the semiquantitive PCR examination of deletion mutant AtYchF1 and wild type Col-2 arabidopsis in the embodiment of the present invention 2
Test result.
Fig. 3 is the chlorophyll content and wild type of deletion mutant AtYchF1 after paraquat treatment in the embodiment of the present invention 3
The chlorophyll content of Col-2 arabidopsis.
Fig. 4 is the comparison of different plant YchF protein amino acid sequences in the embodiment of the present invention 4, homology>90%, its
Middle paddy rice OsYchF1 genes and arabidopsis AtYchF1 genetic homology >=96%.
Specific embodiment
To describe technology contents of the invention, structural feature, the objects and the effects in detail, below in conjunction with implementation method
And coordinate accompanying drawing to be explained in detail.
Fig. 1 to Fig. 4 is refer to, the present invention provides a kind of method of utilization arabidopsis AtYchF1 gene plant weedings, including
Following steps：
Step 1：Cultivate the crop plants of missing AtYchF1 homologous genes；
Step 2：The crop plants of the missing AtYchF1 homologous genes cultivated in field planting step 1；
Step 3：Apply crop plants tolerance, effective dose herbicide in field miscellaneous to control to weeds and crop plants
Grass.
Knowable to foregoing description, the beneficial effects of the present invention are：Because herbicide (paraquat) acts on plant line grain
Body, participates in redox regulatory, produces substantial amounts of active oxygen radical, and inventor is proved by a series of experiments, arabidopsis
AtYchF1 genes also assist in the redox regulatory of inside plants, and take part in plant for herbicide (hundred grass in translation skill
It is withered) resistance, and the extremely strong (homology of protein sequence conservative due to YchF family members between different plant species>90%),
Therefore, for the mutant of AtYchF1 genes, for the industrial crops new varieties for cultivating new herbicide-resistant (paraquat) are provided
New possibility, and then reach using herbicide (paraquat) removing weeds, but the purpose of crops is not injured；This herbicidal methods leads to
Cross and improve crop plants to weeds near the tolerance recycling herbicide removing plant crop of herbicide, both maintained chemistry and removed
The high efficiency of grass, effectively prevent injury of the herbicide (paraquat) to crop again, workable, with certain popularization
Property.
Further, the herbicide is paraquat.
Seen from the above description, use paraquat for herbicide herbicidal effect more preferably.
Further, obtain the AtYchF1 homologous genes crop plants mutant means include RNAi disturb and
CRISPR-Cas6 gene editing technologies.
The present invention also provides application of the arabidopsis AtYchF1 genes in terms of plant weeding, and the AtYchF1 genes exist
Numbering in Genebank is AT1G30580；
The AtYchF1 genes are related to plant resistant herbicide function, after by AtYchF1 gene delections, mutant
Plant is strengthened for the tolerance of herbicide；
And after by AtYchF1 gene overexpressions, mutant plants are lifted for the susceptibility of herbicide.
The course of work of the invention and principle are：G-protein is an important regulatory factor, and wide participation plant is different
Signal transduction pathway.G-protein is divided three classes：Heterotrimeric G-Protein, small G-protein and non-traditional G-protein.With Heterotrimeric G-Protein
As small G-protein, non-traditional G-protein can hydrolyze GTP (GTP) for guanosine diphosphate (GDP) (GDP), therefore have two kinds
Existing forms, with reference to the activation form and the inactive form of combination GDP of GTP.Rice Os YchF1 belongs to non-traditional G-protein, is
A member of G-protein TRAFAC superfamily Obg families YchF subfamilies.
Non-traditional G-protein OsYchF1 is made up of three domains, is followed successively by from N-terminal to C-terminal, double-spiral structure domain, G eggs
White Core domain and TGS (ThrRS, GTPase, SpoT) domain.Core domain is made up of five conservative regions again,
G1, G2, G3, G4 and G5.G1 (GxxxxGK (S/T)) regions can combine GTP or adenosine triphosphate by forming hydrogen bond
(ATP) phosphate radical；G2 (x (T/S) x) and G3 (hhhDxxG) region can combine a magnesium ion, and play hydrolysis GTP
It is the effect of GDP；The identification of guanine or adenine group is assisted in G5 ((T/G) (C/S) A) region；The work(of TGS domains
Can also be not very clear, due to its enriched alkaline amino acid, may participate in combining rRNA；The bird of G4 region recognitions GTP is fast
Purine group, determines G-protein combination GTP or ATP.The G4 regions ((N/T) (M/L) of non-traditional G-protein YchF subfamily members
XE other G-proteins G4 regions ((N/T) KxD)) are different from, the difference in this G4 regions makes OsYchF1 both to combine GTP, and
ATP can be combined, and other types of G-protein can only combine GTP or ATP one kind therein.
Current result of study shows that non-traditional G-protein YchF subfamilies member take part in oxidation in non-transcribed level
Reduction regulation and control, and serve the effect of negative regulatory factor.Osychf1 human body homologue hola1 mutant cells system is demonstrated by
The tolerance of the oxygen pressure caused to tributyl hydroperoxides and diamides, and the oxidation that take part in human body in translation skill is also
Former regulation process.Newest external Biochemical Research result shows, the conservative cysteine of Escherichia coli YchF protein surfaces
(Cys-35) cyclic balance of the protein monomers and homodimer by redox regulatory is take part in, and to its physiology work(
Critical regulating and controlling effect can be served.The Escherichia coli YchF of dimer kenel loses ATPase activity, and sulphur oxygen also egg
Can be interacted with the Escherichia coli YchF of dimer kenel in vain, and recover its ATPase activity.
The early-stage Study result of rice Os YchF1 genes shows, compared with wild type, the plan of rice Os YchF1 genes
Southern mustard overexpression transgenic line is to pathogen (Pseudomonas syringae pv.tomato strain DC3000
(Pst DC3000)) response for sensitivity；Meanwhile, the response to 150mM NaCl treatment is also sensitivity；With wild type (Col-2) phase
Compare, the research of applicant finds that the mutant of AtYchF1 is demonstrated by the resistance to of the active oxygen pressure caused to herbicide (paraquat)
By property.Existing evidence shows that YchF family members take part in the regulation and control that organism tackles stress from outside as negative regulatory factor
Journey, and played an important role in the oxidation-reduction process of response stress.
The present invention is to wild type (Col-2), Gene A tYchF1 afunction mutant representated by AT1G30580
Atychf1 plant find that AtYchF1 genes take part in plant for removing in translation skill in the serial physiological Study to herbicide
The resistance of careless agent.Due to protein sequence conservative extremely strong (homology of the YchF family members between different plant species>90%), pin
To the mutant of AtYchF1 genes, new possibility can be provided to cultivate the industrial crops new varieties of new herbicide-resistant, so as to reach
Weeds are removed to application herbicide, but does not injure the purpose of crops.
Knowable to foregoing description, the beneficial effects of the present invention are：Because herbicide (paraquat) acts on plant line grain
Body, participates in redox regulatory, produces substantial amounts of active oxygen radical, and inventor is proved by a series of experiments, arabidopsis
AtYchF1 genes also assist in the redox regulatory of inside plants, and take part in plant for herbicide (hundred grass in translation skill
It is withered) resistance, and the extremely strong (homology of protein sequence conservative due to YchF family members between different plant species>90%),
Therefore, for the mutant of AtYchF1 genes, for the industrial crops new varieties for cultivating new herbicide-resistant (paraquat) are provided
New possibility, and then reach using herbicide (paraquat) removing weeds, but the purpose of crops is not injured；This herbicidal methods leads to
Cross and improve crop plants to weeds near the tolerance recycling herbicide removing plant crop of herbicide, both maintained chemistry and removed
The high efficiency of grass, effectively prevent injury of the herbicide (paraquat) to crop again, workable, with certain popularization
Property.
Further, the herbicide is paraquat.
Further, the means by AtYchF1 gene delections include that RNAi is disturbed and CRISPR-Cas6 gene editings
Technology.
Further, the RNAi interference is to connect sense the and anti-sense chains of total length AtYchF1 genes respectively
To in RNAi carrier, arabidopsis is infected with titbit infestation method, screening obtains RNAi interference strains.
The further RNAi carrier is pKANNIBAL；
Further, the concrete operations of the RNAi interference are comprised the following steps：
Step 1：Plant is cultivated；
Plant culture includes pinching and watering, and described pinching is to cut off first inflorescence from bottom；After pinching one week, contaminate
The previous day is watered；
Wherein optimum immerged time is：Main inflorescence bears pods, and about 2-10cm is long for secondary inflorescence, there is that a small amount of the flowers are in blossom；
Step 2：Agrobacterium is infected；
The Agrobacterium culture medium that the During Agrobacterium is used is prepared from including following raw material：YEP (10g peptones,
5g yeast extracts, 5g sodium chloride) and 50 μ g/mL antibiotic (kanamycins)；
Agrobacterium condition of culture：In 28 degree of environment, in shaken cultivation under 250rpm rotating speeds；
A) it is inoculated with and selects monoclonal and be inoculated in 10ml YEP culture mediums, in 28~30 degree of environment, turns in 250rpm
The lower shaken cultivation of speed is overnight；
B) in proportion 1:100 are inoculated into 200ml YEP, shake bacterium 18-24 hours so that OD600 is 1.8-2.0；
C) preparation is fresh infects liquid：Including 1/2MS, 5.0% sucrose；0.05%Silwet L-77 (surfactant), pH
=5.7；
D) 5500rpm (rpm) centrifugation, 20min collects thallines are resuspended with liquid is infected so that OD600 is
0.8；
E) infect, portion of tissue immersion on plant is infected liquid 3-5 seconds, and gently rock；
F) sleeve, keeps humidity and lucifuge, overnight；
G) in second day immigration normal growth incubator, until sowing；
Step 3：Screening
A) seed disinfection
I.75% ethanol immersion, strenuous vibration, 2-3 minutes, deionized water rinsing
Ii. sodium hypochlorite immersion, strenuous vibration, 3 minutes, deionization was rinsed five times
B) sow
I. the 1/2MS (Sigma#M-5519) containing resistance is seeded in, on 0.8%agar culture mediums
Further, the CRISPR-Cas6 gene editings technology is to be transferred to selected AtYchF1 genetic fragments
In CRISPR-Cas6 carriers (bgk01), arabidopsis is infected with titbit infestation method.
Embodiment 1
Fig. 1 is refer to, the deletion mutant AtYchF1 and wild type Col-2 that are sprouted on 1/2MS culture mediums five days are intended
Southern mustard goes to continued growth five days on the 1/2MS culture mediums containing 0.1mM paraquats, and observes its phenotype.
As shown in Figure 1, the deletion mutant AtYchF1 for being cultivated on the culture medium containing paraquat is substantially than wild
Type Col-2 arabidopsis grows luxuriant, shows tolerance significantly larger than wild type Col-s of the deletion mutant AtYchF1 to paraquat
2 arabidopsis, that is, the arabidopsis for lacking AtYchF1 genes is improved to the tolerance of paraquat.
Embodiment 2
Fig. 2 is refer to, with sxemiquantitative RT round pcrs, with deletion mutant AtYchF1 and wild type Col-2 arabidopsis
The cDNA for turning is the transcription feelings of masterplate, identification deletion mutant AtYchF1 and wild type Col-2 arabidopsis AtYchF1 gene RNAs
Condition.
As shown in Figure 2, RT-PCR results show do not have the transcription of AtYchF1 gene RNAs in deletion mutant AtYchF1,
And the mutant that wild type Col-2 arabidopsis has the transcription of AtYchF1 gene RNAs, i.e. AtYchF1 does not express AtYchF1's
mRNA。
Embodiment 3
Fig. 3 is refer to, the deletion mutant of five days will be grown on the 1/2MS culture mediums containing 0.1mM paraquats
AtYchF1 and wild type Col-2 Arabidopsis leafs are cut respectively, and 0.8ml N, N-dimehylformamide (DMF) are used at 4 DEG C
Soaked overnight, measures chlorophyll a, chlorophyll b, the content of chlorophyll p, and calculate always containing for chlorophyll with ultraviolet specrophotometer
Amount (ordinate is fluorescence intensity).
From the figure 3, it may be seen that chlorophyll of the chlorophyll content of deletion mutant AtYchF1 higher than wild type Col-2 arabidopsis
Content, shows that AtYchF1 mutant is influenceed less than wild type Col-2 arabidopsis by the active oxygen pressure that paraquat causes.
Embodiment 4
A kind of method of utilization arabidopsis AtYchF1 gene plant weedings, comprises the following steps：
Step 1：Cultivate the crop plants of missing AtYchF1 homologous genes, such as missing rice Os YchF1 gene (homologys
>=rice mutant 96%)；
Step 2：The crop plants of the missing AtYchF1 homologous genes cultivated in field planting step 1, such as in field planting
Paddy rice (missing OsYchF1 genes) mutant that step 1 is cultivated；
Step 3：Apply crop plants tolerance, effective dose herbicide in field miscellaneous to control to weeds and crop plants
Grass.
In sum, application of the arabidopsis AtYchF1 genes that the present invention is provided in terms of plant weeding, due to herbicide
(paraquat) acts on plant mitochondria, participates in redox regulatory, produces substantial amounts of active oxygen radical, and inventor passes through
A series of experiments proves that arabidopsis AtYchF1 genes also assist in the redox regulatory of inside plants, and are participated in translation skill
Resistance of the plant for herbicide (paraquat), and because protein sequence of the YchF family members between different plant species is conservative
Extremely strong (the homology of property>90%), therefore, be to cultivate new herbicide-resistant (paraquat) for the mutant of AtYchF1 genes
Industrial crops new varieties provide new possibility, and then reach using herbicide (paraquat) removing weeds, but do not injure farming
The purpose of thing；This herbicidal methods recycles herbicide removing plant crop attached by improving crop plants to the tolerance of herbicide
Nearly weeds, had both maintained the high efficiency of chemical weed control, and injury of the herbicide (paraquat) to crop is effectively prevent again, operable
Property it is strong, with certain generalization.
Embodiments of the invention are the foregoing is only, the scope of the claims of the invention is not thereby limited, it is every to utilize this hair
Equivalent structure or equivalent flow conversion that bright description and accompanying drawing content are made, or directly or indirectly it is used in other correlations
Technical field, be included within the scope of the present invention.
SEQUENCE LISTING
<110>University Of Agriculture and Forestry In Fujian
<120>Method and application using arabidopsis AtYchF1 gene plant weedings
<130> 2017
<160> 2
<170> PatentIn version 3.5
<210> 1
<211> 1185
<212> DNA
<213>Arabidopsis（Arabidopsis thaliana L.）
<400> 1
atgcctccga aagccaaagc taaagatgca ggtcccgtag agaggcctat tcttggccgt 60
ttctcttctc acctcaagat cggaattgtt gggttaccaa atgttggaaa atctactctt 120
ttcaacactc ttacaaagct ttcaattcca gctgagaatt tccccttttg taccattgag 180
cctaatgagg cacgtgtgaa tatccccgat gagaggttcg attggctttg ccaaacttac 240
aagcctaaga gtgagattcc agctttcttg gaaattcatg acattgctgg gcttgttaga 300
ggagctcatg aaggacaggg tcttggaaac aacttcttgt cccatattcg tgcagttgat 360
ggaattttcc atgttttacg tgcttttgaa gatgctgata ttatccatgt cgacgatatc 420
gtggatcctg ttagagattt ggagaccatt actgaagagt tgcggctaaa ggatattgaa 480
tttgttggaa agaagattga tgatgtcgag aagagcatga agaggagcaa tgacaagcag 540
ctgaaaatag aacttgagct cttgcaaaag gtaaaagctt ggctagaaga tggtaaggat 600
gtccgttttg gggactggaa aacagctgat atcgagattt tgaacacttt ccaattgctt 660
tctgcaaagc ccgttgttta cttgattaac ctgaatgaga gagactatca gaggaagaaa 720
aacaagttct tgccaaagat tcatgcctgg gttcaagaac acggtggtga tactatgatt 780
cctttcagtg gtgtgtttga aaggagtctc gctgatatgg ccccagatga agcagcaaag 840
tattgtgagg agaacaaact gcaaagtgct cttccgagga tcatcaaaac tggattttca 900
gcaattaacc tcatatactt ctttacagca gggcctgatg aggtgaagtg ctggcaaata 960
agacggcagt caaaggctcc tcaagctgca ggggccattc atactgattt tgagagagga 1020
tttatttgtg ctgaggtcat gaaattcgag gatctcaagg aacttggcaa tgaacctgca 1080
gtcaaggccg caggaaaata cagacaggag ggtaaaacat atgttgttca ggatggagat 1140
atcattttct tcaagttcaa tgtttccggt ggtgggaaga aatga 1185
<210> 2
<211> 394
<212> PRT
<213>Arabidopsis（Arabidopsis thaliana L.）
<400> 2
Met Pro Pro Lys Ala Lys Ala Lys Asp Ala Gly Pro Val Glu Arg Pro
1 5 10 15
Ile Leu Gly Arg Phe Ser Ser His Leu Lys Ile Gly Ile Val Gly Leu
20 25 30
Pro Asn Val Gly Lys Ser Thr Leu Phe Asn Thr Leu Thr Lys Leu Ser
35 40 45
Ile Pro Ala Glu Asn Phe Pro Phe Cys Thr Ile Glu Pro Asn Glu Ala
50 55 60
Arg Val Asn Ile Pro Asp Glu Arg Phe Asp Trp Leu Cys Gln Thr Tyr
65 70 75 80
Lys Pro Lys Ser Glu Ile Pro Ala Phe Leu Glu Ile His Asp Ile Ala
85 90 95
Gly Leu Val Arg Gly Ala His Glu Gly Gln Gly Leu Gly Asn Asn Phe
100 105 110
Leu Ser His Ile Arg Ala Val Asp Gly Ile Phe His Val Leu Arg Ala
115 120 125
Phe Glu Asp Ala Asp Ile Ile His Val Asp Asp Ile Val Asp Pro Val
130 135 140
Arg Asp Leu Glu Thr Ile Thr Glu Glu Leu Arg Leu Lys Asp Ile Glu
145 150 155 160
Phe Val Gly Lys Lys Ile Asp Asp Val Glu Lys Ser Met Lys Arg Ser
165 170 175
Asn Asp Lys Gln Leu Lys Ile Glu Leu Glu Leu Leu Gln Lys Val Lys
180 185 190
Ala Trp Leu Glu Asp Gly Lys Asp Val Arg Phe Gly Asp Trp Lys Thr
195 200 205
Ala Asp Ile Glu Ile Leu Asn Thr Phe Gln Leu Leu Ser Ala Lys Pro
210 215 220
Val Val Tyr Leu Ile Asn Leu Asn Glu Arg Asp Tyr Gln Arg Lys Lys
225 230 235 240
Asn Lys Phe Leu Pro Lys Ile His Ala Trp Val Gln Glu His Gly Gly
245 250 255
Asp Thr Met Ile Pro Phe Ser Gly Val Phe Glu Arg Ser Leu Ala Asp
260 265 270
Met Ala Pro Asp Glu Ala Ala Lys Tyr Cys Glu Glu Asn Lys Leu Gln
275 280 285
Ser Ala Leu Pro Arg Ile Ile Lys Thr Gly Phe Ser Ala Ile Asn Leu
290 295 300
Ile Tyr Phe Phe Thr Ala Gly Pro Asp Glu Val Lys Cys Trp Gln Ile
305 310 315 320
Arg Arg Gln Ser Lys Ala Pro Gln Ala Ala Gly Ala Ile His Thr Asp
325 330 335
Phe Glu Arg Gly Phe Ile Cys Ala Glu Val Met Lys Phe Glu Asp Leu
340 345 350
Lys Glu Leu Gly Asn Glu Pro Ala Val Lys Ala Ala Gly Lys Tyr Arg
355 360 365
Gln Glu Gly Lys Thr Tyr Val Val Gln Asp Gly Asp Ile Ile Phe Phe
370 375 380
Lys Phe Asn Val Ser Gly Gly Gly Lys Lys
385 390
Claims (6)
1. using the method for arabidopsis AtYchF1 gene plant weedings, it is characterised in that comprise the following steps：
Step 1：Cultivate the crop plants of missing AtYchF1 homologous genes；
Step 2：The crop plants of the missing AtYchF1 homologous genes cultivated in field planting step 1；
Step 3：Apply crop plants tolerance, effective dose herbicide to weeds and crop plants to control weeds in field.
2. the method for utilization arabidopsis AtYchF1 gene plant weedings according to claim 1, it is characterised in that described
Herbicide is paraquat.
3. the method for utilization arabidopsis AtYchF1 gene plant weedings according to claim 2, it is characterised in that obtain
The means of the AtYchF1 homologous genes include that RNAi is disturbed and CRISPR-Cas6 gene editing technologies.
4. using the application of arabidopsis AtYchF1 gene plant weedings, it is characterised in that the AtYchF1 genes exist
Numbering in Genebank is AT1G30580；
The AtYchF1 genes are related to plant resistant herbicide function, after by AtYchF1 gene delections, mutant plants
Tolerance for herbicide is strengthened；
And after by AtYchF1 gene overexpressions, mutant plants are lifted for the susceptibility of herbicide.
5. the application of utilization arabidopsis AtYchF1 gene plant weedings according to claim 4, it is characterised in that described
Herbicide is paraquat.
6. the application of utilization arabidopsis AtYchF1 gene plant weedings according to claim 5, it is characterised in that described
The means of AtYchF1 gene delections are included that RNAi is disturbed and CRISPR-Cas6 gene editing technologies.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1252221A (en) * | 1998-10-26 | 2000-05-10 | 吴仲芳 | Contacting and sysmtemic wide-spectrum biocidal herbicide-Baicaoxing |
| CN101686642A (en) * | 2007-06-22 | 2010-03-31 | 香港中文大学 | Methods to enhance plant trauma resistance |
| CN106459161A (en) * | 2014-06-20 | 2017-02-22 | 先锋海外公司 | Constructs and methods involving genes encoding glutamate receptor polypeptides |
-
2017
- 2017-03-14 CN CN201710148497.5A patent/CN106868041A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1252221A (en) * | 1998-10-26 | 2000-05-10 | 吴仲芳 | Contacting and sysmtemic wide-spectrum biocidal herbicide-Baicaoxing |
| CN101686642A (en) * | 2007-06-22 | 2010-03-31 | 香港中文大学 | Methods to enhance plant trauma resistance |
| CN106459161A (en) * | 2014-06-20 | 2017-02-22 | 先锋海外公司 | Constructs and methods involving genes encoding glutamate receptor polypeptides |
Non-Patent Citations (5)
| Title |
|---|
| MEIKE WENK ET AL.: "A Universally Conserved ATPase Regulates the Oxidative Stress Response in Escherichia coli", 《THE JOURNAL OF BIOLOGICAL CHEMISTRY》 * |
| MING-YAN CHEUNG ET AL.: "The unconventional P-loop NTPase OsYchF1 and its regulator OsGAP1 play opposite roles in salinity stress tolerance", 《PLANT, CELL AND ENVIRONMENT> * |
| THEOLOGIS,A.ET AL.: "ACCESSION NO:NP_174346,GTP binding protein [Arabidopsis thaliana]", 《GENBANK》 * |
| 苏少泉: "《杂草学》", 31 May 1993 * |
| 袁媛 等: "《传承 创新 发展 首届全国中医药博士后论坛》", 30 November 2009 * |
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