CN106860654A - A kind of utilization fruit of Chinese magnoliavine dregs of a decoction coproduction lignanoid and the method for protein feed - Google Patents
A kind of utilization fruit of Chinese magnoliavine dregs of a decoction coproduction lignanoid and the method for protein feed Download PDFInfo
- Publication number
- CN106860654A CN106860654A CN201710053289.7A CN201710053289A CN106860654A CN 106860654 A CN106860654 A CN 106860654A CN 201710053289 A CN201710053289 A CN 201710053289A CN 106860654 A CN106860654 A CN 106860654A
- Authority
- CN
- China
- Prior art keywords
- fruit
- chinese magnoliavine
- decoction
- lignanoid
- dregs
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/79—Schisandraceae (Schisandra family)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/57—Magnoliaceae (Magnolia family)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses a kind of utilization fruit of Chinese magnoliavine dregs of a decoction coproduction lignanoid and the method for protein feed.First the isolated high-purity lignanoid from the fruit of Chinese magnoliavine dregs of a decoction after water extraction, then passes through solid state fermentation fruit of Chinese magnoliavine residue again, and coproduction obtains the protein feed of high protein content.A kind of high-purity lignanoid that the present invention is provided is the important natural effective active composition of a class; with significantly reducing serum alt, AST levels; slow down degeneration of liver cells necrosis, hence it is evident that mitigate by myocardial damage caused by ischemia-reperfusion, and the multiple efficacies such as calm, analgesia, protection cranial nerve.The protein feed for preparing has protein content high, and fat content is high, nutritious, the advantage of good palatability, and is conducive to animal diseases prevention, improves the effect of immunity of organisms.Simultaneously the fruit of Chinese magnoliavine dregs of a decoction recycle be conducive to economizing on resources, reduces cost, environmental protection.
Description
Technical field
The invention belongs to the technical field of waste resource regeneration, more particularly to fruit of Chinese magnoliavine dregs of a decoction waste resource is made profits again
With, and in particular to reclaimed using the fruit of Chinese magnoliavine dregs of a decoction and obtain high-purity schisandra lignanoid and its residue saccharomycete solid state fermentation coproduction
The technology of high protein feed.
Background technology
The fruit of Chinese magnoliavine is the drying and ripening of magnoliaceae schisandra Schisandra chinensis (Turcz.) Baill.
Fruit, practises and claims " fructus schisandrae ", is clinical compatibility medicine materical crude slice and the life of fruit of Chinese magnoliavine related preparations with abundant medicinal, edibility
One of important source material of product.The generation of the fruit of Chinese magnoliavine dregs of a decoction is mostly derived from Schisandra chinens P.E, preparation, granule and the fruit of Chinese magnoliavine
The manufacturing process of other resource products.These discarded objects are mainly dropped and as waste incineration, not only environment are caused
Serious pollution, wastes the resource of preciousness.And the modern extraction process about fruit of Chinese magnoliavine preparation is more with aqueous extraction-alcohol precipitation technology
Based on, so thing still can be utilized rich in lignanoids, protein-based, carbohydrate and fiber-like etc. in fruit of Chinese magnoliavine solid-state castoff
Matter, with value very high, is worth further developing and resource.Therefore, resource is carried out again to the fruit of Chinese magnoliavine dregs of a decoction
There is important ecological significance and economic implications using being recycled with system.
At present, existing researcher find can be fermented with Chinese medicine slag culture edible mushroom, with Chinese medicine slag fertilizer processed, use Chinese medicine
Slag solid state fermentation produces protein feed and with Chinese medicine slag papermaking and prepares flocculant etc..By various biotechnologys by these
Medicine waste material is processed, turning waste into wealth has been increasingly becoming a feasible path, wherein using Chinese medicine slag solid state fermentation
Production feed, can not only alleviate environmental pollution, can also reduce the wasting of resources, and especially active functional component in the dregs of a decoction, makes
Thing feed possesses diseases prevention, effect that is disease-resistant and improving animal immunizing power, as animal health-care product feed of new generation.
Fermentation technology process often relates to the growth and breeding of microbial cell, the synthesis of product and tight association therewith
The biochemical reaction that is catalyzed of various enzymes, form and physiological habit, the population behavior of microorganism are all by the shadow of technological condition for fermentation
Ring.Pair biological factor related to fermentation and condition of culture are optimized, and can find an optimal zymotechnique, together
When also save fermentation costs.
Chinese medicine slag fermentation is turned into the feed addition of the marine organisms such as animal nutrition feed, Yi Jiyu, shrimp using microorganism
Agent, is currently a popular recycling approach.Because in the fruit of Chinese magnoliavine dregs of a decoction containing a large amount of albumen, polysaccharide etc. can using nutrition into
Point, and albumen, polyoses content are greatly improved in the dregs of a decoction after fermenting, therefore meat can be produced using fruit of Chinese magnoliavine dregs of a decoction solid state fermentation
Fruit of Chinese magnoliavine dregs of a decoction fermentation culture medium, can also be added to the marine organisms such as fish, sea shrimp by the protein feed of the animals such as chicken, piglet
In nutrient fodder, can not only alleviate environmental pollution, can also reduce the wasting of resources, animal feed, marine organisms feed also possess
Diseases prevention, effect that is disease-resistant and improving animal immunizing power, this is great existing to having recycled for environmental protection and fruit of Chinese magnoliavine residue
Sincere justice.
The content of the invention
Goal of the invention：The purpose of the present invention is directed to what this reproducible utilization resource treatment of the current fruit of Chinese magnoliavine dregs of a decoction was present
Waste and problem of environmental pollution, there is provided one kind utilizes fruit of Chinese magnoliavine dregs of a decoction co-producing high-purity fruit of Chinese magnoliavine lignanoid and the solid state fermentation five tastes
The method that sub- residue obtains high protein feed, the method can effectively realize that recycling for the fruit of Chinese magnoliavine dregs of a decoction utilizes with system,
Economize on resources, reduce environmental pollution.Extracting the high-purity schisandra lignanoid for reclaiming can make as enhance immunity, liver protection
The raw material of health food or feed addictive；Fruit of Chinese magnoliavine residue after alcohol extracting is fermented by saccharomycete solid state fermentation and made
The protein feed of standby high protein content, good palatability, and be conducive to animal diseases prevention, improve the effect of immunity of organisms.
The purpose of the present invention is achieved by the following technical solution：
A kind of utilization fruit of Chinese magnoliavine dregs of a decoction coproduction lignanoid and the method for protein feed, first reclaim from the fruit of Chinese magnoliavine dregs of a decoction and obtain
High-purity schisandra lignanoid, its residue passes through saccharomycete solid state fermentation coproduction high protein feed again.Can efficient system utilization
The discarded fruit of Chinese magnoliavine dregs of a decoction, reduce environmental pollution, realize that resource is maximum and utilize and recycle.
Preferably, the above is comprised the following steps with the method for fruit of Chinese magnoliavine dregs of a decoction coproduction lignanoid and protein feed：
(1) it is particle that the fruit of Chinese magnoliavine dregs of a decoction after water will be used to extract are dried, crushed；
(2) fruit of Chinese magnoliavine dregs of a decoction particle is put into extractor, adds 8~16 times of dregs of a decoction weight of 60%~90% volume
The ethanol of concentration, refluxing extraction 1~3 time, 60~120min, obtains ethanol extract every time；
(3) ethanol extract is concentrated, is concentrated into w/v 3：1~1：1, obtain concentrate；
(4) by large pore resin absorption column on concentrate, the ethanol of use is eluted, and collects eluent, and concentration obtains five
Taste total lignan extract；
(5) fruit of Chinese magnoliavine residue after extracting lignanoid through step (2) ethanol is dried, it is standby；
(6) prepared by culture medium：The culture medium of preparation includes plating medium, fermented bean drink culture medium, solid-state fermentation culture medium；
(7) solid state fermentation：Candida tropicalis (Candida tropicalis) are cultivated 12 on plating medium
~36h, after saccharomycete grows, is made thalline suspension, so with oese inoculation yeast bacterium in the murphy juice culture medium for preparing
After be placed in shaking table after 8~12h, take a certain amount of thalline suspension in another murphy juice culture medium, be placed in 12 in shaking table~
20h；Then saccharomycete is inoculated in solid-state fermentation culture medium by 8%~12% inoculum concentration, then in constant incubator
Culture 3d~7d, fermentation is completed；
(8) material by completion of fermenting is dried, and obtains final product solid-state protein feed.
Preferably, the method for optimizing of high-purity lignanoid is in utilization fruit of Chinese magnoliavine dregs of a decoction preparation provided above：
Fruit of Chinese magnoliavine dregs of a decoction particle (40 mesh) is put into extractor, 10 times of dregs of a decoction weight of 90% ethanol is added, refluxing extraction 2 times,
Each 120min, obtains ethanol extract；
Ethanol extract is concentrated under reduced pressure, and is concentrated into w/v 2：1, obtain concentrate；
By D101 large pore resin absorption columns on concentrate, carry out eluting 3 times of eluents of column volume using 95% ethanol,
Obtain purity be 40.8% fructus schizandrae total lignans extract, wherein schizandrin A 5.8%, deoxyschizandrin 11.9%,
Schisandrin C 3.6%, schizandrin 12.8%, wuweizi alcohol B 6.7%.
Preferably, the fruit of Chinese magnoliavine residue through after alcohol extracting of the present invention passes through saccharomycete solid state fermentation coproduction again
The method of high protein feed,
The preparation of culture medium：Including plating medium, fermented bean drink culture medium, solid-state fermentation culture medium.
A. plating medium：In the pure water of 1.0L add 10g glucose, 10g peptones, 5g yeast extracts and
The agar of 20g.Sterilize 30min in 121 DEG C of high-pressure sterilizing pots, for the flat board culture of Candida tropicalis.
B. fermented bean drink culture medium：200mg potatoes are taken, the pure water of 1.8mL or so is added, the potato for being made 1.0L is boiled in heating
Juice, is put into 20.0g glucose, is divided in the open conical flask of 250mL, and sterilize 30min in 120 DEG C of high-pressure sterilizing pots, uses
In the Liquid Culture of Candida tropicalis.
C. solid-state fermentation culture medium：Raw material includes by weight：80~100 parts of fruit of Chinese magnoliavine residue, ammonium sulfate 3~10
Part, 0.5~1 part of potassium dihydrogen phosphate, 0.3~1.5 part of magnesium sulfate, 0.5~1.5 part of sodium chloride, 0.1~0.5 part of calcium chloride, wheat bran
10~50 parts, feed liquid mass ratio is 1:1~1:3.Sterilized 30min in 120 DEG C of high-pressure sterilizing pots, and 10~30 times are added after cooling
Cellulase (ultraviolet sterilization) stirring of residue.
Used as more preferred scheme, the solid-state fermentation culture medium preparation method that the present invention is provided is：Fruit of Chinese magnoliavine residue 100g,
40 mesh sieves are crossed, 4g ammonium sulfate, 0.5g potassium dihydrogen phosphates, 0.5g magnesium sulfate, 0.5g sodium chloride, 0.1g calcium chloride is then sequentially added
With 20g wheat bran, feed liquid mass ratio is 1:2.Sterilized 30min in 120 DEG C of high-pressure sterilizing pots, and 3000mg celluloses are added after cooling
Enzyme (ultraviolet sterilization) is stirred.
Preferably, the solid-state fermentation process method of present invention offer is：Candida tropicalis are trained in flat board
Support and cultivate 24h on base, after saccharomycete grows, with oese 2 ring saccharomycete of inoculation in the 50mL murphy juice culture mediums for preparing
Thalline suspension is made, is placed in shaking table after 10h, take the thalline suspension of 2mL in another 50mL murphy juices culture medium, be placed in and shake
16h in bed.The saccharomycete that inoculum concentration is 10% is added in solid-state fermentation culture medium, is then 30 DEG C incubated in temperature
5d is cultivated in case.
Beneficial effect：The present invention has advantages below：
A kind of utilization fruit of Chinese magnoliavine dregs of a decoction coproduction lignanoid and the method for protein feed that the present invention is provided, first from fruit of Chinese magnoliavine medicine
Reclaimed in slag and obtain high-purity schisandra lignanoid, its residue passes through saccharomycete solid state fermentation coproduction high protein feed again.This hair
Bright energy efficient system reduces environmental pollution using the discarded fruit of Chinese magnoliavine dregs of a decoction, realizes the maximum utilization of resource and recycles.Institute
The high-purity lignanoid for stating is the important natural effective active composition of a class, with serum alt, AST levels is significantly reduced, is subtracted
Slow degeneration of liver cells necrosis, hence it is evident that mitigate by myocardial damage caused by ischemia-reperfusion, and calm, analgesia, protection cranial nerve
Etc. multiple efficacies.Can be used for acute, chronic hepatitis patient, the health care of ischemic heart disease patient, preventive and therapeutic effect.The coproduction
A kind of fruit of Chinese magnoliavine protein feed has protein content high, and fat content is high, nutritious, the advantage of good palatability, and favorably
In animal diseases prevention, the effect of raising immunity of organisms.
Specific embodiment
With reference to specific embodiment, the present invention is expanded on further.However, as it will be easily appreciated by one skilled in the art that real
Apply the specific material proportion described by example, process conditions and its result and be merely to illustrate the present invention, without that will not also should limit
The present invention described in detail in claims processed.All technologies realized based on the above of the present invention belong to the present invention
Scope.Embodiment 1
A kind of utilization fruit of Chinese magnoliavine dregs of a decoction coproduction lignanoid and the method for protein feed, described method pass through following steps reality
It is existing：
(1) fruit of Chinese magnoliavine dregs of a decoction pulverizer is crushed and is screened to 20 mesh.
(2) fruit of Chinese magnoliavine dregs of a decoction particle is put into extractor, adds 8 times of dregs of a decoction weight of 70% ethanol, refluxing extraction 3
Secondary, each 90min obtains ethanol extract；Ethanol extract is concentrated under reduced pressure, and is concentrated into w/v 1：1, obtain dense
Contracting liquid；By AB-8 large pore resin absorption columns on concentrate, carry out eluting 5 times of eluents of column volume using 80% ethanol, obtain
Purity is 36.6% fructus schizandrae total lignans extract, wherein schizandrin A 5.6%, the deoxyschizandrin 10.1%, five tastes
Sub- C prime 3.4%, schizandrin 11.3%, wuweizi alcohol B 6.2%.
(3) fruit of Chinese magnoliavine residue through after alcohol extracting of the present invention passes through saccharomycete solid state fermentation coproduction high protein feed again
Method, comprise the following steps：
1. the preparation of culture medium：Including plating medium, fermented bean drink culture medium, solid-state fermentation culture medium.
A. plating medium：In the pure water of 1.0L add 10g glucose, 10g peptones, 5g yeast extracts and
The agar of 20g.Sterilize 30min in 121 DEG C of high-pressure sterilizing pots, for the flat board culture of Candida tropicalis.
B. fermented bean drink culture medium：200mg potatoes are taken, the pure water of 1.8mL or so is added, the potato for being made 1.0L is boiled in heating
Juice, is put into 20.0g glucose, is divided in the open conical flask of 250mL, and sterilize 30min in 120 DEG C of high-pressure sterilizing pots, uses
In the Liquid Culture of Candida tropicalis.
C. solid-state fermentation culture medium：Fruit of Chinese magnoliavine residue 80g, crosses 40 mesh sieves, then sequentially adds 4g ammonium sulfate, 0.5g phosphoric acid
Potassium dihydrogen, 0.5g magnesium sulfate, 0.5g sodium chloride, 0.1g calcium chloride and 20g wheat bran, feed liquid mass ratio are 1:3.In 120 DEG C of high pressures
Sterilized 30min in autoclave, and 2500mg cellulases (ultraviolet sterilization) stirrings are added after cooling.
2. solid ferment process：Candida tropicalis are cultivated into 20h on plating medium, after saccharomycete grows,
2 ring saccharomycete are inoculated with oese and are made thalline suspension in the 50mL murphy juice culture mediums for preparing, be placed in shaking table after 8h, take
The thalline suspension of 2mL is placed in 12h in shaking table in another 50mL murphy juices culture medium.Added in solid-state fermentation culture medium
Inoculum concentration is 8% saccharomycete, then cultivates 3d in the constant incubator that temperature is 30 DEG C.
3. the material by completion of fermenting is dried, and obtains final product solid-state protein feed.Wherein total protein content 20.4%, reduction
Sugared content 9.2%, content of starch 8.2%.
Embodiment 2
A kind of utilization fruit of Chinese magnoliavine dregs of a decoction coproduction lignanoid and the method for protein feed, it is characterized in that, described method passes through
Following steps are realized：
(1) fruit of Chinese magnoliavine dregs of a decoction pulverizer is crushed and is screened to 40 mesh.
(2) fruit of Chinese magnoliavine dregs of a decoction particle is put into extractor, adds 10 times of dregs of a decoction weight of 90% ethanol, refluxing extraction
2 times, each 120min obtains ethanol extract；Ethanol extract is concentrated under reduced pressure, and is concentrated into w/v 2：1, obtain
Concentrate；By D101 large pore resin absorption columns on concentrate, carry out eluting 3 times of eluents of column volume using 95% ethanol,
Obtain purity position 40.8% fructus schizandrae total lignans extract, wherein schizandrin A 5.8%, deoxyschizandrin 11.9%,
Schisandrin C 3.6%, schizandrin 12.8%, wuweizi alcohol B 6.7%.
(3) fruit of Chinese magnoliavine residue through after alcohol extracting of the present invention passes through saccharomycete solid state fermentation coproduction high protein feed again
Method, comprise the following steps：
1. the preparation of culture medium：Including plating medium, fermented bean drink culture medium, solid-state fermentation culture medium.
A. plating medium：In the pure water of 1.0L add 10g glucose, 10g peptones, 5g yeast extracts and
The agar of 20g.Sterilize 30min in 121 DEG C of high-pressure sterilizing pots, for the flat board culture of Candida tropicalis.
B. fermented bean drink culture medium：200mg potatoes are taken, the pure water of 1.8mL or so is added, the potato for being made 1.0L is boiled in heating
Juice, is put into 20.0g glucose, is divided in the open conical flask of 250mL, and sterilize 30min in 120 DEG C of high-pressure sterilizing pots, uses
In the Liquid Culture of Candida tropicalis.
C. solid-state fermentation culture medium：Fruit of Chinese magnoliavine residue 100g, crosses 40 mesh sieves, then sequentially adds 4g ammonium sulfate, 0.5g phosphorus
Acid dihydride potassium, 0.5g magnesium sulfate, 0.5g sodium chloride, 0.1g calcium chloride and 20g wheat bran, feed liquid mass ratio are 1:2.It is high in 120 DEG C
Sterilized 30min in pressure autoclave, and 3000mg cellulases (ultraviolet sterilization) stirrings are added after cooling.
2. solid ferment process：Candida tropicalis are cultivated into 24h on plating medium, after saccharomycete grows,
2 ring saccharomycete are inoculated with oese and are made thalline suspension in the 50mL murphy juice culture mediums for preparing, be placed in shaking table after 10h,
The thalline suspension of 2mL is taken in another 50mL murphy juices culture medium, 16h in shaking table is placed in.Add in solid-state fermentation culture medium
Enter the saccharomycete that inoculum concentration is 10%, then cultivate 5d in the constant incubator that temperature is 30 DEG C.
3. the material by completion of fermenting is dried, and obtains final product solid-state protein feed.Wherein total protein content 25.2%, reduction
Sugared content 11.3%, content of starch 10.6%.
Embodiment 3
A kind of utilization fruit of Chinese magnoliavine dregs of a decoction coproduction lignanoid and the method for protein feed, it is characterized in that, described method passes through
Following steps are realized：
(1) fruit of Chinese magnoliavine dregs of a decoction pulverizer is crushed and is screened to 40 mesh.
(2) fruit of Chinese magnoliavine dregs of a decoction particle is put into extractor, adds 12 times of dregs of a decoction weight of 80% ethanol, refluxing extraction
2 times, each 100min obtains ethanol extract；Ethanol extract is concentrated under reduced pressure, and is concentrated into w/v 3：1, obtain
Concentrate；By D101 large pore resin absorption columns on concentrate, carry out eluting 3 times of eluents of column volume using 70% ethanol, obtain
Obtain the fructus schizandrae total lignans extract that purity is 39.7%, wherein schizandrin A 5.6%, deoxyschizandrin 11.1%, five
Taste C prime 4.4%, schizandrin 12.7%, wuweizi alcohol B 5.9%.
(3) fruit of Chinese magnoliavine residue through after alcohol extracting of the present invention passes through saccharomycete solid state fermentation coproduction high protein feed again
Method, comprise the following steps：
1. the preparation of culture medium：Including plating medium, fermented bean drink culture medium, solid-state fermentation culture medium.
A. plating medium：In the pure water of 1.0L add 10g glucose, 10g peptones, 5g yeast extracts and
The agar of 20g.Sterilize 30min in 121 DEG C of high-pressure sterilizing pots, for the flat board culture of Candida tropicalis.
B. fermented bean drink culture medium：200mg potatoes are taken, the pure water of 1.8mL or so is added, the potato for being made 1.0L is boiled in heating
Juice, is put into 20.0g glucose, is divided in the open conical flask of 250mL, and sterilize 30min in 120 DEG C of high-pressure sterilizing pots, uses
In the Liquid Culture of Candida tropicalis.
C. solid-state fermentation culture medium：Fruit of Chinese magnoliavine residue 80g, crosses 40 mesh sieves, then sequentially adds 4g ammonium sulfate, 0.5g phosphoric acid
Potassium dihydrogen, 0.5g magnesium sulfate, 0.5g sodium chloride, 0.1g calcium chloride and 20g wheat bran, feed liquid mass ratio are 1:3.In 120 DEG C of high pressures
Sterilized 30min in autoclave, and 2500mg cellulases (ultraviolet sterilization) stirrings are added after cooling.
2. solid ferment process：Candida tropicalis are cultivated into 36h on plating medium, after saccharomycete grows,
2 ring saccharomycete are inoculated with oese and are made thalline suspension in the 50mL murphy juice culture mediums for preparing, be placed in shaking table after 12h,
The thalline suspension of 2mL is taken in another 50mL murphy juices culture medium, 10h in shaking table is placed in.Add in solid-state fermentation culture medium
Enter the saccharomycete that inoculum concentration is 9%, then cultivate 5d in the constant incubator that temperature is 30 DEG C.
3. the material by completion of fermenting is dried, and obtains final product solid-state protein feed.Wherein total protein content 21.3%, reduction
Sugared content 12.1%, content of starch 9.3%.
The anti-hepatic injury pharmacological evaluation of embodiment 4
First, experiment material and medicine
1. medicine and reagent
AST, ALT, MDA, SOD kit and Coomassie brilliant blue protein reagent box are purchased from Nanjing and build up bio-engineering research
Institute；Carbon tetrachloride (CCl4, analyze pure, Shanghai Ling Feng chemical reagent Co., Ltd), it is configured to 0.1% with peanut oil when using
Peanut oil solution；Bifendate (Jiangsu Pengyao Pharmaceutical Co., Ltd.), faces that the used time is ground into fine powder plus physiological saline is made suspension
Liquid.
2. experimental animal
Cleaning grade male ICR mouse, 18~22g of body weight is provided by Zhejiang Province's Experimental Animal Center, quality certification number SCXK
(Zhejiang) 2008-0033.
3. laboratory apparatus
Enzyme linked immunological instrument (Bio-Tek companies of the U.S.)；UV-2000 types ultraviolet specrophotometer (Beijing Lai Baitaike instruments
Co., Ltd)；BT125 types electronic balance (Sai Duolisi scientific instrument Co., Ltd)；KQ-250E type ultrasonic cleaner (elder brothers
Shan Hechuan ultrasonic instruments Co., Ltd)；Anke GL-16GII types centrifuge (Anting Scientific Instrument Factory, Shanghai).
4. test medicine and processing method
The fructus schizandrae total lignans that Example 1~3 is prepared respectively, are diluted with water and are made required concentration；Positive drug
It is DDB, required concentration is configured to distill water dissolves before administration.
2nd, experimental technique
1. couple CCl4Cause the influence of acute liver
60 mouse are taken, after adaptability is fed 1 week, 6 groups, respectively every group 10, normal control is randomly divided into by body weight
Group, CCl4To liver injury model group, the fructus schizandrae total lignans group (0.25g/kg/d) that embodiment 1,2 and 3 is prepared, biphenyl
Dibasic acid esters group (120mg/kg/d).Control group and model group give the physiological saline of same amount, administration group continuous gavage 10d, in last
1h after administration, in addition to normal group ip 0.9%NaCl 10mL/kg, the equal ip 0.1%CCl of remaining each group4Peanut oil solution 10mL/
kg.After 16h, all mouse separate serum using retroorbital venous clump blood sampling, determine the activity of serum alt and AST.Take blood
After put to death mouse, partial liver is taken immediately, 10% LH is prepared into physiological saline, according to kit explanation determine MDA
Content and SOD vigor.
2. statistical procedures
All experimental datas carry out statistical procedures using SPSS11.5 statistical processing softwares, as a result withRepresent, group
Between compare and use variance analysis.
3rd, experimental result
1. fructus schizandrae total lignans are to CCl4Cause the influence of acute liver
CCl4Liver injury model group compares with normal group, mice serum ALT, AST and the significantly raised (P of hepatic tissue MDA levels
<0.01), SOD activity substantially reduces (P<0.01)；Fructus schizandrae total lignans group, biphenyl pair that embodiment 1,2 and 3 is prepared
Ester group compares with model group and make to some extent hepatic injury mice serum ALT, AST reduction (P<0.01)；Fruit of Chinese magnoliavine hammer butt fat
Element group and DDB group compare with model group and make to some extent hepatic injury murine liver tissue MDA levels reduction (P<
0.05), SOD levels raise (P<0.05).Specific experiment the results are shown in Table 1.
The each group of table 1 is to CCl4Cause acute hepatic injury mice Serum ALT, AST and liver SOD, MDA influence (mean ± SD,
N=10)
Note：Compare with model group,##P<0.01,#P<0.05。
The myocardial damage pharmacological evaluation of embodiment 5
First, experiment material and medicine
1. medicine and reagent
MDA, SOD, NO kit are purchased from Nanjing and build up Bioengineering Research Institute.
2. experimental animal
Healthy male SD rat, 222~240g of body weight is provided by Zhejiang Province's Experimental Animal Center, quality certification number SCXK
(Zhejiang) 2008-0033.
3. test medicine and processing method
The fructus schizandrae total lignans that Example 1~3 is prepared respectively, dilution is made required concentration；Positive drug is connection
Benzene dibasic acid esters, dissolving is configured to required concentration before administration.
2nd, experimental technique
1. the foundation of Model of Myocardial Ischemia-Reperfusion Injury and materials
Each group animal pre-operative anxiety 24 hours, 20% urethane (1g.kg-1) intraperitoneal anesthesia, trachea cannula connects lung ventilator,
Mechanical ventilation；Trace limb and lead electrocardiogram；Chest is opened at left border of sternum 2mm, pericardium is cut off, coronary artery is exposed, after left anterior descending branch starting point
0.5cm wears a silk thread, and the silicone tube of pad one is ligatured during blood pressure stabilization, closes thoracic cavity.Ligation is injected through tail vein after 45 minutes and is treated
Medicine and solution, medicine group press 10g.Kg-1(pressing the calculating of humans and animals body weight ratio, be 5 times of clinical dosage), uses physiology
Salt solution is diluted to 3.0ml, is injected intravenously before Reperfu- sion, and remaining two groups are injected with normal saline.Then thoracic cavity is opened, is cut off
Ligature, sham-operation group is not ligatured after putting line, and coronary artery stops and whether leads to again and raised according to ECG ST section and recover to be defined.Again
After perfusion 1 hour, the detection that SOD, MDA, NO index are done after the left room sample disposal in part is taken.
2. the influence of the amplitude and incidence of arrhythmia of pair ischemical reperfusion injury rat T ripples change
Record rat ligature preceding 5 minutes and ligature after 1,10,20 minutes and 1,5,30 minutes, the heart of 3 hours after filling again
Electrograph (LII), calculates the mV numbers that T ripples are raised and reduced, and the amplitude that T ripples change is compared by 1mv=20mm with its absolute value
Compared with.Tachycardia, ventricular fibrillation and T ripples change the incidence more than 1mm to be included to the total incidence of arrhythmia cordis simultaneously
Counted.
3. the influence that MDA, SOD and NO are generated in pair myocardial ischemia-reperfusion tissue
Rat heart prepares myocardial homogenates supernatant, then by kit operating instruction, MDA, SOD in cardiac muscle is determined respectively
With NO contents.
3rd, experimental result
1. the influence of the incidence of the amplitude and arrhythmia cordis of pair ischemical reperfusion injury Rat Ecg T ripples change, greatly
Mouse LADCA is filled 1 hour again after ligaturing 45 minutes, can cause the myocardial ischemia-reperfusion injury of rat, is showed
The T ripples of electrocardiogram change, and incidence of arrhythmia is significantly raised；Fructus schizandrae total lignans can significantly inhibit rat after filling again
The amplitude of ECG T wave change and the incidence of arrhythmia cordis.
2. the influence that MDA, SOD and NO are generated in pair Ischemic/reperfused Myocardium tissue
Model group MDA and NO content are significantly reduced apparently higher than sham-operation group, the vigor of SOD, and comparing with sham-operation group has
Significant difference (P<0.01).Compare with model group, the fructus schizandrae total lignans group that embodiment 1~3 is prepared can reduce cardiac muscle
The generation of middle MDA, statistically significant (P<0.05)；The vigor of SOD in Reperfu- sion tissue, difference highly significant can be significantly improved
(P<0.01).Generation (the P of NO can be suppressed<0.05) 2, are shown in Table.
The change of the rat heart muscle tissue MDA contents SOD of table 2 activity and NO contents
Note：Compare with model group,##P<0.01,#P<0.05。
Embodiment 6
By the solid-state protein feed that the method for the embodiment of the present invention 1,2 and 3 is prepared, be added in feed feed respectively it is young
Each 50 of pig, 3 groups of piglets monthly increase weight respectively 14 kilograms, 15 kilograms and 15 kilograms, morbid state do not occur.The present invention is prepared
Fruit of Chinese magnoliavine protein feed have protein content high, fat content is high, nutritious, the advantage of good palatability, and is conducive to
Animal diseases prevention, the effect for improving immunity of organisms.
A kind of utilization fruit of Chinese magnoliavine dregs of a decoction coproduction lignanoid and the method for protein feed that the present invention is provided, obtaining for being obtained are high
Purity lignanoid has significantly reduces the multiple efficacies such as liver protection, calmness, analgesia, protection cranial nerve.Can be used for acute, chronic hepatitis disease
People, the health care of ischemic heart disease patient, preventive and therapeutic effect.The fruit of Chinese magnoliavine protein feed for being obtained has protein content high,
It is nutritious, good palatability, and be conducive to animal diseases prevention, improve the effect of immunity of organisms.While the system of the fruit of Chinese magnoliavine dregs of a decoction
Using and recycle be conducive to economizing on resources, reduces cost, environmental protection.
Claims (9)
1. a kind of method of utilization fruit of Chinese magnoliavine dregs of a decoction coproduction lignanoid and protein feed, it is characterised in that first from after water extraction
Isolated high-purity lignanoid in the fruit of Chinese magnoliavine dregs of a decoction, then again by solid state fermentation fruit of Chinese magnoliavine residue, coproduction obtains high protein
The protein feed of content.
2. the method for utilization fruit of Chinese magnoliavine dregs of a decoction coproduction lignanoid according to claim 1 and protein feed, it is characterised in that
Comprise the following steps：
(1) it is particle that the fruit of Chinese magnoliavine dregs of a decoction after water will be used to extract are dried, crushed；
(2) fruit of Chinese magnoliavine dregs of a decoction particle is put into extractor, adds 8~16 times of dregs of a decoction weight of 60%~90% volumetric concentration
Ethanol, refluxing extraction 1~3 time, every time 60~120min obtains ethanol extract；
(3) ethanol extract is concentrated, is concentrated into w/v 3：1~1：1, obtain concentrate；
(4) by large pore resin absorption column on concentrate, the ethanol of use is eluted, and collects eluent, and concentration obtains the fruit of Chinese magnoliavine
Total lignan extract；
(5) fruit of Chinese magnoliavine residue after extracting lignanoid through step (2) ethanol is dried, it is standby；
(6) prepared by culture medium：The culture medium of preparation includes plating medium, fermented bean drink culture medium, solid-state fermentation culture medium；
(7) solid state fermentation：Candida tropicalis are cultivated into 12~36h on plating medium, after saccharomycete grows, with connecing
Kind of ring inoculation yeast bacterium is made thalline suspension in the murphy juice culture medium for preparing, and is subsequently placed in shaking table after 8~12h, takes one
Quantitative thalline suspension is placed in 12~20h in shaking table in another murphy juice culture medium；Then 8%~12% inoculation is pressed
Be inoculated in saccharomycete in solid-state fermentation culture medium by amount, and 3d~7d is then cultivated in constant incubator, and fermentation is completed；
(8) material by completion of fermenting is dried, and obtains final product solid-state protein feed.
3. the method for utilization fruit of Chinese magnoliavine dregs of a decoction coproduction lignanoid according to claim 2 and protein feed, it is characterised in that
In step (2), by the fruit of Chinese magnoliavine dregs of a decoction using 8 times~12 times of 60%~90% volumetric concentration ethanol, refluxing extraction 2~3 times,
Each 80min~120min, obtains ethanol extract.
4. the method for utilization fruit of Chinese magnoliavine dregs of a decoction coproduction lignanoid according to claim 2 and protein feed, it is characterised in that
D101 or AB-8 large pore resin absorption columns on concentrate, washing for 2~5 times of column volumes of wash-out is carried out using 60%~95% ethanol
Precipitation liquid, obtains fructus schizandrae total lignans extract of the purity more than 35%, wherein schizandrin A 3%~6%, fruit of Chinese magnoliavine second
Element 5%~11%, schisandrin C 2%~4%, schizandrin 7%~13%, wuweizi alcohol B 4%~9%.
5. the method for utilization fruit of Chinese magnoliavine dregs of a decoction coproduction lignanoid according to claim 2 and protein feed, it is characterised in that
Total protein content 18%~50%, content of reducing sugar 8%~12%, content of starch 5%~12% in step (8).
6. the method for utilization fruit of Chinese magnoliavine dregs of a decoction coproduction lignanoid according to claim 2 and protein feed, it is characterised in that
Solid-state fermentation culture medium is prepared as follows：Take 80~100 parts of fruit of Chinese magnoliavine residue, 3~10 parts of ammonium sulfate, biphosphate
10~50 parts of 0.5~1 part of potassium, 0.3~1.5 part of magnesium sulfate, 0.5~1.5 part of sodium chloride, 0.1~0.5 part of calcium chloride and wheat bran,
Mixing, sterilized 30min in 120 DEG C of high-pressure sterilizing pots, and 10~30 times of cellulase stirrings of residue are added after cooling, is obtained.
7. the method for utilization fruit of Chinese magnoliavine dregs of a decoction coproduction lignanoid according to claim 2 and protein feed, it is characterised in that
Plating medium is to prepare as follows：10g glucose, 10g peptones, 5g yeast is added to carry in the pure water of 1.0L
Take the agar of thing and 20g.Sterilize 30min in 121 DEG C of high-pressure sterilizing pots, for the flat board culture of Candida tropicalis.
8. the method for utilization fruit of Chinese magnoliavine dregs of a decoction coproduction lignanoid according to claim 2 and protein feed, it is characterised in that
Fermented bean drink culture medium is to prepare as follows：200mg potatoes are taken, the pure water of 1.8mL or so is added, heating is boiled and is made
The murphy juice of 1.0L, is put into 20.0g glucose, is divided in the open conical flask of 250mL, is gone out in 120 DEG C of high-pressure sterilizing pots
Bacterium 30min, for the Liquid Culture of Candida tropicalis.
9. the lignanoid that claim 1 is prepared is in for preparing preventing and treating acute, chronic hepatitis, ischemic heart disease medicine
Application.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710053289.7A CN106860654A (en) | 2017-01-18 | 2017-01-18 | A kind of utilization fruit of Chinese magnoliavine dregs of a decoction coproduction lignanoid and the method for protein feed |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710053289.7A CN106860654A (en) | 2017-01-18 | 2017-01-18 | A kind of utilization fruit of Chinese magnoliavine dregs of a decoction coproduction lignanoid and the method for protein feed |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106860654A true CN106860654A (en) | 2017-06-20 |
Family
ID=59158707
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710053289.7A Pending CN106860654A (en) | 2017-01-18 | 2017-01-18 | A kind of utilization fruit of Chinese magnoliavine dregs of a decoction coproduction lignanoid and the method for protein feed |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106860654A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107712406A (en) * | 2017-11-02 | 2018-02-23 | 上海创博生态工程有限公司 | A kind of chicken feed prepared and preparation method thereof of being fermented using fruit of Chinese magnoliavine industrial wood waste |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103478413A (en) * | 2013-09-18 | 2014-01-01 | 中国林业科学研究院林产化学工业研究所 | Method for producing protein feed by mixed-strain solid-state fermentation of ginkgo leaf residues |
-
2017
- 2017-01-18 CN CN201710053289.7A patent/CN106860654A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103478413A (en) * | 2013-09-18 | 2014-01-01 | 中国林业科学研究院林产化学工业研究所 | Method for producing protein feed by mixed-strain solid-state fermentation of ginkgo leaf residues |
Non-Patent Citations (1)
| Title |
|---|
| 陶小芳: "生脉注射液生产过程五味子药渣的资源化利用研究", 《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107712406A (en) * | 2017-11-02 | 2018-02-23 | 上海创博生态工程有限公司 | A kind of chicken feed prepared and preparation method thereof of being fermented using fruit of Chinese magnoliavine industrial wood waste |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104256584B (en) | A kind of Moringa ferment and preparation method thereof | |
| CN106255421B (en) | Method for producing garlic fermented composition and garlic fermented composition | |
| CN112430546B (en) | Heterotrophic fermentation preparation method and application of chlamydomonas reinhardtii and chlamydomonas reinhardtii powder | |
| CN106047560A (en) | Preparation method of tartary buckwheat rice wine | |
| CN106387924A (en) | Method for extracting dietary fibers for suaeda salsa | |
| CN110200151A (en) | A kind of yak feed addictive and preparation method thereof that yak survival rate can be improved | |
| CN107319196A (en) | A kind of microorganism formulation for improving fresh-water fishes function of intestinal canal and application thereof | |
| CN105876764A (en) | Preparation technology of fermented mushroom sauce | |
| CN107549817A (en) | A kind of natural organic calcium of Moringa and preparation method thereof | |
| CN106614306B (en) | A kind of chicken method for breeding | |
| CN108669348A (en) | A kind of mulberry bar microbiological feed and preparation method thereof | |
| CN105962369A (en) | Method for preparing lactobacillus fermentation liquor from black tea enzymatic hydrolysate | |
| CN104522750A (en) | Alga healthcare food | |
| CN108523112B (en) | Silkworm chrysalis food base material and preparation method and application thereof | |
| CN103947871A (en) | Meet goose compound feed and preparation method thereof | |
| CN107865267A (en) | A kind of preparation method of penaeus vannamei boone feed additive | |
| CN106860654A (en) | A kind of utilization fruit of Chinese magnoliavine dregs of a decoction coproduction lignanoid and the method for protein feed | |
| CN100488381C (en) | Propolis ganoderma lucidum spore, dried black fungus polysaccharide compounded soft capsule and preparing process therefor | |
| CN110656151A (en) | Method for improving kidney-tonifying and yang-strengthening effects of sea cucumber | |
| CN110101081A (en) | Nutrition Elastin peptide and preparation method thereof | |
| CN105535035A (en) | Inonotus obliquus fermentation culture composition and preparation method thereof | |
| CN109198123A (en) | A kind of Phellinus health protection tea and preparation method thereof | |
| CN107927366A (en) | New disease-resistant lamb supplement material of height and preparation method thereof | |
| CN114916626A (en) | Prebiotics fish feed containing traditional Chinese medicine oligosaccharide and mulberry leaf oligosaccharide and application thereof | |
| CN110200290A (en) | A kind of extracting method of Chinese yam polypeptide |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170620 |