CN106854625A - A kind of microbial bacterial agent for oils degradation and preparation method thereof - Google Patents
A kind of microbial bacterial agent for oils degradation and preparation method thereof Download PDFInfo
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- CN106854625A CN106854625A CN201710027747.XA CN201710027747A CN106854625A CN 106854625 A CN106854625 A CN 106854625A CN 201710027747 A CN201710027747 A CN 201710027747A CN 106854625 A CN106854625 A CN 106854625A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/845—Rhizopus
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
- B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/885—Trichoderma
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Abstract
The invention discloses a kind of microbial bacterial agent for oils degradation and preparation method thereof, the microbial inoculum is main to be made up of Chinese Rhizopus oryzae and long shoot trichoderma by following mass ratio row：Chinese Rhizopus oryzae 30 70%, long shoot trichoderma 70 30%, the living bacteria count of the microbial inoculum is 5 × 108‑3×109CFU. microbial bacterial agent strong adaptability of the invention, oil substances that can be in efficient degradation kitchen garbage.Can be degraded in 48h more than 90% greasy dirt.
Description
Technical field
The invention belongs to Applied Biotechnology field, technical field of environmental microorganism is particularly related to.
Background technology
Modern industrialization exacerbates environmental pollution, the exploitation of oil and the production and operation of petrochemical enterprise while development
Activity all causes the substantial amounts of grease pollution of enterprise's Soil Surrounding and river.On the other hand eat with the improvement of people ' s living standards
The amount of kitchen rubbish also gradually increases.There is substantial amounts of grease in kitchen garbage, the presence of grease brings to the treatment of kitchen garbage
Huge difficulty.General microorganism is unable to growth and breeding in the grease of high concentration under normal circumstances, and grease can not lead to
Common micro-organisms degraded is crossed, this causes huge to kitchen garbage biological treatment treatment and oil-polluted soils and water remediation
Obstacle.The microorganism that efficient degradation grease how is filtered out from by grease type contaminated soil has become current environment pollution
The hot research problem of improvement.
The content of the invention
In order to overcome the shortcomings of that above-mentioned conventional environment quasi-microorganism can not degrade grease, the invention provides（One kind is used for
Microbial bacterial agent of oils degradation and preparation method thereof）.The method is separated to two groups from the environment polluted by grease can be high
The fungi of effect degraded grease, identifies that this two plants of bacterium are respectively Chinese Rhizopus oryzae and product branch trichoderma through 16SDNA, appoints in this two plants of bacterium
One single bacterium is high to the degradation efficiency of grease, and the degradation rate to olive oil in 48h is able to reach 63% and 68%, by surveying
Trying both bacterium can yielding lipase in certain degree.Both bacterium are combined according to certain ratio, both
Degraded of the bacterium to grease can play synergy well, and the degradation rate to olive oil in 48h can reach more than 75%.
The technical solution adopted in the present invention is：Microbial bacterial agent for oils degradation and preparation method thereof its feature exists
It is in the mass percent of each component microorganism in microbial bacterial agent gross mass of, the pretreatment microbial bacterial agent：Chinese rice
Head mold 20-70 components, the ratio combination of long shoot trichoderma 30-80 components.The described microbial bacterial agent for oils degradation, it is special
Levy and be：The living bacteria count of the microbial inoculum is 6 × 108-7×109CFU, and the number of viable of every kind of single bacterium is not less than 1 × 108
CFU.Lipase activity is in 20-50u/g.
Described microbial bacterial agent for oils degradation and preparation method thereof, it is characterised in that the method is by each component
Microorganism is compounded so as to obtain the micro- of efficient degradation grease after carrying out solid state fermentation culture respectively according still further to certain ratio row
Bacteria agent.
Described microbial bacterial agent for oils degradation and preparation method thereof, it is characterised in that it includes following step
Suddenly：
a：The preparation of first order seed：Chinese Rhizopus oryzae and long shoot trichoderma strain are inoculated in PDA respectively and are incubated at 100-400rpm,
Under conditions of 28-45 DEG C, cultivate 2-3 days, treat that seed maturity is standby
b：The preparation of secondary seed：The first order seed that will have been activated, is inoculated into liquid fermentation tank according to the inoculum concentration of 10%-20%
Ferment, fermentation is：28-45 DEG C of temperature, throughput is controlled to 0.5-2L/min, speed of agitator 300-500rpm, dress
Material coefficient is controlled to 75%, ferments 3-5 days, prepares secondary seed stand-by
c：Solid fermentation is produced：Chinese Rhizopus oryzae and long shoot trichoderma secondary seed are inoculated in solid medium are respectively consolidated
Body ferments, and in 50-80%, at 28-40 DEG C, culture prepares Chinese rice root for 4-6 days to cultivation temperature to control solid medium moisture
The single microbial inoculum of mould and long shoot trichoderma.
d：Microbial inoculum is combined：It is according to mass percent by Chinese Rhizopus oryzae and long shoot trichoderma：Chinese Rhizopus oryzae 30-70%, length
Branch trichoderma 70-30% ratios carry out being mixed with the microbial bacterial agent obtained for kitchen garbage grease degraded.
Culture medium in step b used by secondary seed constitutes and is：The wheat bran of 2-5%, the glucose of 1-2%, remaining is water.Step
Solid medium is wheat bran in rapid c：Rice bran is 4:6-7:3, dregs of beans 2-5%, glucose 1-3%.
Beneficial effect of the present invention：
Compared with prior art, the beneficial effects of the invention are as follows its technology of high-efficiency grease degradation bacterial agent and product for preparing
Product have following Some features：
1) technique employs the technique that liquid state fermentation is combined with solid state fermentation, and fermentation seed, solid-state work are prepared under the conditions of liquid
Final finished product is prepared under the conditions of skill, with process is simple, equipment investment expense is low, and low production cost is not substantial amounts of
Waste water is produced.
2) microbial inoculum has living bacteria count amount high, and every gram of effective viable bacteria of product reaches as high as 6 × 108-7×109CFU, treatment
Same amount of material can reduce the dosage of microbial inoculum so as to be greatly reduced operating cost.
3）Screened from the environment polluted by grease respectively using strain, the applicable ability of product is strong, can adapt to each
The place of quasi-grease pollution.
4）The fast growth of strain, odor removal efficient is high, and the obvious degradation effect to greasy dirt can be played in 48h, from
And efficient repair is played to environment
Strain properties and function selected by the present invention are as follows：
1) Chinese Rhizopus oryzae：The bacterium can not only utilize grease, and can also produce substantial amounts of starch under certain conditions
Enzyme and saccharification enzyme, the enzyme activity of carbohydrase may be up to 2000u/g
2）Long shoot trichoderma：In addition to it can produce lipase, itself also has the characteristic of High Cellulase Production to the bacterium, certain
Under conditions of the cellulose enzyme activity up to 200u/g that is produced of the bacterium, cellulose substances can be played with the energy of fast degradation
Power
3) composite bacteria agent：Both bacterium are compound can not only to play quickly degraded left and right to polluting the grease in environment, and
Small point of carbohydrate and aliphatic acid, glycerine etc. can also be translated into the degraded of cellulose and the starch organic matter high in environment
Sub- material.Quick repair can be played to greasy dirt and Organic Pollution environment.
The present invention is further elaborated by the following examples
First, test tube slant culture medium
PDA culture medium：The potato 150g of skin is removed, with filter of making amends for one's faults less after the 1 boiling tap water 1h of addition that is cut into small pieces, filter is collected
Liquid addition 15g glucose is settled to 1L regulation pH to 6.5, and addition 20g agar boils to agar and melts, be sub-packed in test tube, goes out
Bacterium, prepares inclined-plane standby.
2nd, liquid primary-seed medium：It is formulated with test tube slant culture medium, without agar
3rd, microbial inoculum production medium：The corn flour of 2-5%, the glucose of 1-2%, remaining is water to corn pulp 5-10%, is sterilized standby.
Embodiment 1
Respectively from the soil sample of refuse landfill and the oil serious pollution for filling kitchen garbage, sample is diluted with sterilized water
50 times of 200RPM on bed is wanted, under conditions of, shake 1-2h, cultivated 5 days in so accessing PDA liquid medium with 5% inoculum concentration
So 5 times repeatedly, pregnant solution is then diluted to 10-8-10-10Times, coat on the flat board of PDA selectivity, select form typical case
Single bacterium colony carry out test tube slant preservation, and censorship 16S RNA identifications.This laboratory screens A, B degraded grease effect altogether
Preferably, identified A China Rhizopus oryzae, B are long shoot trichoderma.
Embodiment 2
1) 4 DEG C of strains of preservation are carried out into slant activation：Chinese Rhizopus oryzae and long shoot trichoderma are inoculated in PDA inclined-planes in 37 DEG C
Under the conditions of carry out activation 3 days
2) preparation of primary seed solution：Respectively from the Chinese Rhizopus oryzae and long shoot trichoderma of picking activation on the test tube slant of activation,
It is inoculated in PDA liquid medium in 35 DEG C, the lower culture of 200rpm 3 days obtains liquid first order seed.
3）The preparation of secondary seed：Respectively by step 2）Chinese Rhizopus oryzae and long shoot trichoderma are cultivated, according to 10% inoculation
Amount is fermented in being inoculated into liquid fermentation tank, and fermentation is：40 DEG C of temperature, throughput is controlled to 2.5L/min, and stirring turns
Fast 250rpm, coefficient is controlled to 75%, and pressure 0.15MPa ferments 4 days, prepares secondary seed stand-by
4）Solid fermentation is produced：Secondary seed is inoculated in respectively carry out solid fermentation in solid medium, controls solid culture
85%, at 40 DEG C, culture prepares the single microbial inoculum of Chinese Rhizopus oryzae, long shoot trichoderma for 5 days to cultivation temperature to base moisture.
5）Microbial inoculum is combined：It is according to weight ratio by Chinese Rhizopus oryzae and long shoot trichoderma：20 parts of Chinese Rhizopus oryzae, long shoot trichoderma
80 parts of ratio be mixed with and obtains grease high efficiency degradation bacterial agent
Effective viable bacteria content is 3 × 10 in the high-efficiency grease microbial inoculum for degrading for being obtained9CFU.Lipase active is in 40U/
G, diastatic activity is in 106U/g, cellulase activity 150U/g.
Embodiment 3
Detection method
1）The preparation of standard curve
0,0.2,0.4,0.6,0.8,1.0,1.2,1.4,1.6,1.8 g/ are respectively configured by solvent of the petroleum ether of 60-90 boiling ranges
The standard liquid of L concentration, determines the absorbance of each solution under conditions of 230nm, and coordinate is attached most importance to concentration as horizontal with absorbance
Coordinate is mapped to obtain standard curve.
2）The detection of sample：
Sample is centrifuged 10min and takes supernatant by sampling under conditions of 3000rpm, with 1:1 sulphur bar acid is acidified to below pH1 and adds
Enter sodium chloride and use solution saturation, solution is moved into separatory funnel, with 50ml petroleum ethers（Divide 2-3 times）By oil extraction out,
Extract detects absorbance after accessing 50ml volumetric flask constant volumes under 230nm wavelength.
3）Experimental result：
The making of 3.1 standard curves
| Grease concentration g/L | Absorbance A |
| 0.2 | 0.001 |
| 0.4 | 0.053 |
| 0.6 | 0.111 |
| 0.8 | 0.172 |
| 1 | 0.216 |
| 1.2 | 0.268 |
| 1.4 | 0.323 |
| 1.6 | 0.38 |
| 1.8 | 0.443 |
Embodiment 4
Long shoot trichoderma is individually tested
4 bottles of 50ml of configuration, are inoculated with bacterium long shoot trichoderma 10ml, sample the spectrophotometry outside in 24,48,60,72h respectively
Fat content in sample.
Culture medium is constituted（Oil-containing waste water）：Ammonium sulfate 0.1g/L;Potassium dihydrogen phosphate 0.02g/L;Dipotassium hydrogen phosphate 0.1g/
L;Sodium chloride 1g/L；Olive oil 2g/L
Experiment condition：The corresponding time is cultivated on 30 DEG C of shaking tables of 150rpm.
Experimental result is as shown in the table
| 0h | 24h | 48h | 60h | 72h | |
| Sample absorbance | 0.48 | 0.39 | 0.122 | 0.121 | |
| Grease concentration g/L | 2 | 1.88 | 1.628 | 0.64 | 0.639 |
| Oil and grease degradation rate % |
By data above it can be seen that produce branch trichoderma individually reached 68% after the 3rd day more by force to the degradation capability of olive oil.But
Speed is not too fast.
Embodiment 5
Chinese Rhizopus oryzae is individually tested
4 bottles of 50ml of configuration, the Chinese Rhizopus oryzae 10ml of inoculation, sample the spectrophotometry outside in 24,48,60,72h respectively
Fat content in sample.
Culture medium is constituted（Oil-containing waste water）：Ammonium sulfate 0.1g/L;Potassium dihydrogen phosphate 0.02g/L;Dipotassium hydrogen phosphate 0.1g/
L;Sodium chloride 1g/L；Olive oil 2g/L
Experiment condition：The corresponding time is cultivated on 30 DEG C of shaking tables of 150rpm.
Experimental result is as shown in the table
| Process time | 0h | 24h | 48h | 60h | 72h |
| Sample absorbance | 0.46 | 0.21 | 0.130 | 0.091 | |
| Grease concentration g/L | 2 | 1.37 | 0.97 | 0.67 | 0.53 |
| Oil and grease degradation rate % | Initial value | 31.5 | 51.5 | 66.5 | 73 |
By data above it can be seen that Chinese Rhizopus oryzae to the degradation capability of the relatively strong grease after 2 days of degradation capability of olive oil compared with
More than 50% was just reached at the 2nd day by force, more than 70% has been reached in the degraded to olive oil in 72 hours.
Embodiment 6
Chinese Rhizopus oryzae and product branch trichoderma combination experiment
4 bottles of 50ml of configuration, inoculation bacterium long shoot trichoderma 2ml, Chinese Rhizopus oryzae 8ml, respectively in 24,48,60,72h samplings outside
Fat content in spectrophotometry sample.
Culture medium is constituted（Oil-containing waste water）：Ammonium sulfate 0.1g/L;Potassium dihydrogen phosphate 0.02g/L;Dipotassium hydrogen phosphate 0.1g/
L;Sodium chloride 1g/L；Olive oil 2g/L
Experiment condition：The corresponding time is cultivated on 30 DEG C of shaking tables of 150rpm.
Experimental result is as shown in the table
| Process time | 0h | 24h | 48h | 60h | 72h |
| Sample absorbance | 0.32 | 0.092 | 0.07 | 0.122 | |
| Grease concentration g/L | 2 | 1.37 | 0.535 | 0.45 | 0.64 |
| Oil and grease degradation rate % | Initial value | 31.5 | 73.25 | 77.5 | 68 |
By data above it can be seen that head mold and long shoot trichoderma combine the drop to the relatively strong grease after 2 days of degradation capability of olive oil
Solution ability just reaches more than 70%.It was to have reached 77.5% at 60 hours.Head mold and the combination of long shoot bar trichoderma as can be seen here has stronger
Oils degradation ability.
Brief description of the drawings:
Fig. 1 is " oils degradation standard curve " figure；Fig. 2 is " each bacterium and combinations thereof is compared the degradation rate of olive oil " figure.
Claims (7)
1. a kind of microbial bacterial agent for oils degradation and preparation method thereof, it is characterised in that the pretreatment microbial bacterial agent
Mass percent of each component microorganism in quality in microorganism be：Chinese Rhizopus oryzae 30-70%, long shoot trichoderma 70-30%.
2. according to claim 1（A kind of microbial bacterial agent for oils degradation and preparation method thereof）, its feature exists
In：Total effective viable bacteria content is 5 × 10 in every gram of microbial bacterial agent8-3×109CFU, and every kind of single bacterium number of viable not
Less than 2 × 108 CFU。
3. a kind of microbial bacterial agent for oils degradation as claimed in claim 1 or 2 and preparation method thereof, it is characterised in that
The process characteristic that the method is combined using liquid with solid state fermentation：Seed liquor is prepared under conditions of liquid, in the bar of solid-state
Single bacterium solid pharmaceutical preparation is prepared under part, the single bacterium microbial inoculum compounding that then will be prepared again prepares finished product microbial bacterial agent.
4. described microbial bacterial agent for oils degradation and preparation method thereof is required according to right 3, it is characterised in that it is wrapped
Include the steps：
a：The preparation of first order seed：Chinese Rhizopus oryzae and long shoot trichoderma strain are inoculated in PDA respectively and are incubated at 100-400rpm,
Under conditions of 28-45 DEG C, cultivate 2-3 days, treat that seed maturity is standby
b：The preparation of secondary seed：The first order seed that will have been activated, is inoculated into liquid fermentation tank according to the inoculum concentration of 10%-20%
Ferment, fermentation is：28-45 DEG C of temperature, throughput is controlled to 0.5-2L/min, speed of agitator 300-500rpm, dress
Material coefficient is controlled to 75%, ferments 3-5 days, prepares secondary seed stand-by
c：Solid fermentation is produced：Chinese Rhizopus oryzae and long shoot trichoderma secondary seed are inoculated in solid medium are respectively consolidated
Body ferments, and in 50-80%, at 28-40 DEG C, culture prepares Chinese rice root for 4-6 days to cultivation temperature to control solid medium moisture
The single microbial inoculum of mould and long shoot trichoderma.
5.d：Microbial inoculum is combined：It is according to mass percent by Chinese Rhizopus oryzae and long shoot trichoderma：Chinese Rhizopus oryzae 30-70%, long shoot
Trichoderma 70-30% ratios carry out being mixed with the microbial bacterial agent obtained for kitchen garbage grease degraded.
6. preparation method according to claim 4, it is characterised in that the culture medium composition in step b used by secondary seed
For：The wheat bran of 2-5%, the glucose of 1-2%, remaining is water.
7. preparation method according to claim 4, it is characterised in that solid medium is wheat bran in step c：Rice bran is 4:
6-7:3, dregs of beans 2-5%, glucose 1-3%.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107964518A (en) * | 2017-12-25 | 2018-04-27 | 江苏世邦生物工程科技有限公司 | A kind of complex micro organism fungicide and its preparation method and application |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102533562A (en) * | 2011-09-27 | 2012-07-04 | 江苏科技大学 | Rhizopus oryzae TY GF1 bacterial strain and application thereof in degrading high-concentration oil in catering waste water |
-
2017
- 2017-01-17 CN CN201710027747.XA patent/CN106854625A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102533562A (en) * | 2011-09-27 | 2012-07-04 | 江苏科技大学 | Rhizopus oryzae TY GF1 bacterial strain and application thereof in degrading high-concentration oil in catering waste water |
Non-Patent Citations (4)
| Title |
|---|
| S.PAPANIKOLAOU等: "biotechnological conversion of waste cooking oil into lipid-rich biomass using Aspergillus and penicillium strains", 《JOURNAL OF APPLIED MICROBIOLOGY》 * |
| 范文斌等: "《发酵工艺技术》", 31 December 2014 * |
| 金志刚: "《污染物生物降解》", 29 November 1997 * |
| 隋聪颖等: "产脂肪酶木霉的分离筛选鉴定及酶学性质研究", 《生物技术通报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107964518A (en) * | 2017-12-25 | 2018-04-27 | 江苏世邦生物工程科技有限公司 | A kind of complex micro organism fungicide and its preparation method and application |
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