CN106841500B - The thin-layered chromatography detection method of baked ginger in a kind of female biochemical mixture of benefit - Google Patents
The thin-layered chromatography detection method of baked ginger in a kind of female biochemical mixture of benefit Download PDFInfo
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- CN106841500B CN106841500B CN201710123654.7A CN201710123654A CN106841500B CN 106841500 B CN106841500 B CN 106841500B CN 201710123654 A CN201710123654 A CN 201710123654A CN 106841500 B CN106841500 B CN 106841500B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/90—Plate chromatography, e.g. thin layer or paper chromatography
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Abstract
The invention discloses the thin-layered chromatography detection methods of baked ginger in a kind of female biochemical mixture of benefit；Solve in the prior art that solvent is there are security risk, and the problem that detection effect is not ideal enough.The present invention includes the preparation of (1) test solution: taking beneficial female biochemical mixture that test solution is made；(2) drawing test solution point, expansion, taking-up is dried as solvent on silica gel thin-layer plate, using petroleum ether, ethyl acetate and methylene chloride that ratio is 2: 1: 1；(3) it is placed under ultraviolet lamp and inspects.The present invention improves detection accuracy, and keeps detection safer, and effect is very significant.
Description
Technical field
The present invention relates to a kind of thin-layered chromatography detection methods, and in particular to the thin layer color of baked ginger in a kind of female biochemical mixture of benefit
Spectrum detection method.
Background technique
Beneficial female biochemical mixture by motherwort, Radix Angelicae Sinensis, Rhizoma Chuanxiong, peach kernel, baked ginger, the above Six-element medicinal material processing of Radix Glycyrrhizae (toast) and
At.Have effects that promoting blood circulation, antalgic, is mainly used for treating disease that postpartum lochia can't go, abdominal pain due to blood stasis.It is greenish orange yellow
To brown color liquid.Gas is fragrant, slightly sweet.The female biochemical mixture of the benefit is mainly used for the livestocks such as horse, ox, sheep, pig, wherein horse, Niu Yong
Amount is 200-300mL, and sheep, pig dosage are 30-50mL.
Said preparation is recorded in " veterinary drug national standard compilation --- veterinary drug provincial standard rises national standard " (second at present
Volume), indentification by TLC only is carried out to motherwort, Radix Glycyrrhizae, Rhizoma Chuanxiong in quality standard, no content measuring standard is difficult to control
Its quality is made, guarantees safety, validity and the quality controllability of product.
During thin layer identifies under " Republic of China Veterinary Pharmacopoeia " 2010 editions second baked ginger items, using with petroleum
Ether-ethyl acetate-chloroform (2:1:1) is solvent, is unfolded, and takes out, dries, and is sprayed with vanillin-sulfuric acid test solution, at 105 DEG C
It is heated to spot development clearly method.
In aforesaid way, the chloroform that when detection uses is more toxic, and is two class precursor chemicals, and operation has peace
Full hidden danger.Also, it is detected using aforesaid way, detection effect is not ideal enough.
Summary of the invention
The technical problems to be solved by the present invention are: there are security risks for solvent in the prior art, and detection effect is not
Enough ideal problems, and it is an object of the present invention to provide in a kind of female biochemical mixture of the benefit to solve the above problems baked ginger thin-layer chromatography detection side
Method.
The present invention is achieved through the following technical solutions:
The thin-layered chromatography detection method of baked ginger in a kind of female biochemical mixture of benefit, comprising:
(1) preparation of test solution: take beneficial female biochemical mixture as test solution；
(2) test solution point is drawn in petroleum ether, ethyl acetate and two on silica gel thin-layer plate, with ratio for 2: 1: 1
Chloromethanes is unfolded as solvent, takes out, dries；
(3) it is placed under ultraviolet lamp and inspects.
By the optimization of above-mentioned solvent and the optimization of detection mode, the accuracy rate of testing result is effectively improved, and
Effectively avoid the security performance that detection is improved using chloroform.
Further, the silica gel thin-layer plate uses silica gel g thin-layer plate.The wavelength of the ultraviolet lamp is 365nm.
Further, the boiling range specification of the petroleum ether is 60~90 DEG C.Also point has control on the silica gel thin-layer plate
Medicinal material solution and negative sample solution, wherein the negative sample solution is the female biochemical mixture prescription medicine of the benefit in addition to baked ginger
According to liquid made of beneficial female biochemical mixture preparation process；The control medicinal material solution is using baked ginger according to beneficial female biochemical mixture system
Liquid made of standby technique.
In the prior art in the thin-layer chromatography detection for carrying out baked ginger, since there are other compositions, other compositions in drug
Effect can be interfered to composition to be measured, thus in different drugs, although composition to be measured is the same, it is suitable for one of which
The preprocessing means of drug are not necessarily suitable another drug.Also, inventor has found the drug of same materials composition, system
When standby technique has differences, extreme influence will also result in final finished, especially for using thin-layer chromatography detection wherein certain
One group of timesharing, testing result very different, the testing result that above-mentioned factor also results in thin-layer chromatography detection are not ideal enough.
The present invention effectively increases detection accuracy by the selection of optimization control medicinal material solution and negative sample solution.
Compared with prior art, the present invention having the following advantages and benefits:
Optimization design through the invention improves detection accuracy, and keeps detection safer.
Detailed description of the invention
Fig. 1 is that the thin-layer chromatography of embodiment 1 detects figure.
Fig. 2 is that the thin-layer chromatography of embodiment 2 detects figure.
Specific embodiment
To make the objectives, technical solutions, and advantages of the present invention clearer, below with reference to embodiment, the present invention is made
Further to be described in detail, exemplary embodiment of the invention and its explanation for explaining only the invention, are not intended as to this
The restriction of invention.
Embodiment 1
The thin-layered chromatography detection method of baked ginger, specific detection process are as follows in a kind of female biochemical mixture of benefit:
Beneficial female biochemical mixture is taken to be used as test solution in right amount.1/20 of the prescription medicine amount except baked ginger is taken to give birth to by beneficial mother
Negative control solution is made in bonding agent preparation process.Separately take baked ginger control medicinal material appropriate, by the female biochemical mixture preparation process system of benefit
At control medicinal material solution.6-gingerol reference substance is taken, adds ethyl acetate that reference substance solution is made.
Above-mentioned each 10 μ L of four kinds of solution is drawn respectively, is put respectively on same silica gel g thin-layer plate, with petroleum ether, acetic acid second
Ester and methylene chloride are solvent, wherein petroleum ether: ethyl acetate: methylene chloride=2:1:1, the temperature of petroleum ether is 60~
90 DEG C, it is unfolded, takes out, dry, sets and inspected under the ultraviolet lamp under the conditions of 365nm.The result is shown in Figure 1.In Fig. 1, marked as 1,2
It is negative control solution, what it is marked as 3,4 is the control medicinal material solution of baked ginger, and what it is marked as 5,6 is test solution, marked as
7,8 be reference substance solution.
Testing result:
In sample chromatogram, on position corresponding with control medicinal material solution and reference substance solution chromatography, same color is shown
Fluorescence spot, it is negative noiseless.
Embodiment 2
The present embodiment is the comparative examples of embodiment 1, and solvent and detection mode are different in the present embodiment, specific to examine
Survey process is as follows:
Beneficial female biochemical mixture is taken to be used as test solution in right amount.1/20 of the prescription medicine amount except baked ginger is taken to give birth to by beneficial mother
Negative control solution is made in bonding agent preparation process.Separately take baked ginger control medicinal material appropriate, by the female biochemical mixture preparation process system of benefit
At control medicinal material solution.6-gingerol reference substance is taken, adds ethyl acetate that reference substance solution is made.
Above-mentioned each 10 μ L of four kinds of solution is drawn respectively, is put respectively on same silica gel g thin-layer plate, with petroleum ether, acetic acid second
Ester and chloroform are solvent, wherein petroleum ether: ethyl acetate: the boiling range specification of chloroform=2:1:1, petroleum ether is
60~90 DEG C, it is unfolded, takes out, dry, sprays with vanillin-sulfuric acid test solution, it is clear to be heated to spot development at 105 DEG C.As a result see figure
2.In Fig. 2, what it is marked as 1,2 is negative control solution, and what it is marked as 3,4 is the control medicinal material solution of baked ginger, marked as 5,6
It is test solution, what it is marked as 7,8 is reference substance solution.
Testing result:
In sample chromatogram, on position corresponding with control medicinal material solution and 6-gingerol reference substance solution chromatography,
The spot of aobvious same color, it is negative noiseless, but can not detect that other compositions, testing result are not accurate enough in baked ginger.
Above-described specific embodiment has carried out further the purpose of the present invention, technical scheme and beneficial effects
It is described in detail, it should be understood that being not intended to limit the present invention the foregoing is merely a specific embodiment of the invention
Protection scope, all within the spirits and principles of the present invention, any modification, equivalent substitution, improvement and etc. done should all include
Within protection scope of the present invention.
Claims (2)
1. the thin-layered chromatography detection method of baked ginger in a kind of female biochemical mixture of benefit characterized by comprising
(1) preparation of test solution: take beneficial female biochemical mixture as test solution；
(2) test solution point is drawn on silica gel thin-layer plate, the petroleum ether, ethyl acetate and the dichloromethane that are 2: 1: 1 with ratio
Alkane is unfolded as solvent, takes out, dries；
(3) it is placed under ultraviolet lamp and inspects；
The silica gel thin-layer plate uses silica gel g thin-layer plate；
The wavelength of the ultraviolet lamp is 365nm；
The boiling range specification of the petroleum ether is 60~90 DEG C.
2. the thin-layered chromatography detection method of baked ginger in the female biochemical mixture of a kind of benefit according to claim 1, which is characterized in that
Also point has control medicinal material solution and negative sample solution on the silica gel thin-layer plate, wherein the negative sample solution is except baked ginger
The female biochemical mixture prescription medicine of benefit in addition is according to liquid made of beneficial female biochemical mixture preparation process；The control medicinal material solution is
Using baked ginger according to liquid made of beneficial female biochemical mixture preparation process.
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| CN201710123654.7A CN106841500B (en) | 2017-03-03 | 2017-03-03 | The thin-layered chromatography detection method of baked ginger in a kind of female biochemical mixture of benefit |
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| CN106841500B true CN106841500B (en) | 2018-12-28 |
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| JP3816262B2 (en) * | 1998-09-30 | 2006-08-30 | 花王株式会社 | Ceramide production promoter |
| CN101337060B (en) * | 2008-08-08 | 2012-08-22 | 广西博科药业有限公司 | Quality control method of Gongyankang grannule |
| GB2463531B (en) * | 2008-09-23 | 2012-08-08 | Kenneth Davison | The sub-critical water extraction of Baikal Skullcap |
| WO2010034971A2 (en) * | 2008-09-23 | 2010-04-01 | Gary William Wheatley | Sub-critical water extraction of medicinal plants |
| RU2572337C2 (en) * | 2014-06-05 | 2016-01-10 | Государственное бюджетное образовательное учреждение высшего профессионального образования "Самарский государственный медицинский университет" Министерства здравоохранения Российской Федерации | Method for identification of ginger plant syrup |
| CN105259295B (en) * | 2015-11-17 | 2017-05-17 | 山东沃华医药科技股份有限公司 | Quality detection method for ginseng, cassia twig and poria cocos oral solution |
| CN105664099A (en) * | 2016-02-03 | 2016-06-15 | 河南省南山菇业有限公司 | Traditional Chinese medicine composition for warming and invigorating kidney yang and preparation method thereof |
| CN105853968A (en) * | 2016-04-28 | 2016-08-17 | 刘华 | Ginger and folium artemisiae argyi scraping agent and preparation method thereof |
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