CN105738553B - The thin-layered chromatography detection method of lophatherum gracile in a kind of qingwen baidu powder - Google Patents
The thin-layered chromatography detection method of lophatherum gracile in a kind of qingwen baidu powder Download PDFInfo
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- CN105738553B CN105738553B CN201610080410.0A CN201610080410A CN105738553B CN 105738553 B CN105738553 B CN 105738553B CN 201610080410 A CN201610080410 A CN 201610080410A CN 105738553 B CN105738553 B CN 105738553B
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/90—Plate chromatography, e.g. thin layer or paper chromatography
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Abstract
The invention discloses a kind of thin-layered chromatography detection method of lophatherum gracile in qingwen baidu powder；Solve the problems, such as that qingwen baidu powder is poor with lophatherum gracile thin-layer identification method effect in the prior art.The present invention includes（1）Need testing solution is prepared using qingwen baidu powder；（2）Need testing solution point is drawn on silica gel thin-layer plate, be 2.2: 4.8: 2.5: 0.8: 1.5 dimethylbenzene, ethyl acetate, acetone, formic acid and water for solvent using ratio, expansion, taking-up, dries；（3）Put and inspected under ultraviolet lamp.The present invention have expanding performance it is excellent, in qingwen baidu powder other constituents on lophatherum gracile Detection results without influence the advantages that.
Description
Technical field
The present invention relates to a kind of detection method, and in particular to the thin-layer chromatography detection side of lophatherum gracile in a kind of qingwen baidu powder
Method.
Background technology
Qingwen baidu powder is stupid the formulated recipe of teacher more than the famous warm disease scholar of the Qing Dynasty, and it is written to be loaded in its《A View of Epidemic Febrile Diseases with Rashes》
In one book, now it is embodied in《Republic of China Veterinary Pharmacopoeia》Version two in 2010.This medicine is by gypsum, glutinous rehmannia, cornu bubali, the coptis
Formed Deng 14 taste medicinal materials, be " Baihu Tang ", " huanglian jiedu decoction " and " xijiao Dihuang Tang " tripartite addition or subtraction of changes and obtain.Eliminating pestilence and relieving toxicity
Scattered prescription includes:Gypsum 120g, radix rehmanniae recen 30g, cornu bubali 60g, coptis 20g, cape jasmine 30g, moutan bark 20g, radix scutellariae
25g, radix paeoniae rubrathe 25g, radix scrophulariae 25g, wind-weed 30g, capsule of weeping forsythia 30g, balloonflower root 25g, radix glycyrrhizae 15g, lophatherum gracile 25g；It is cool with purging intense heat and detonicating
The effect of blood, clinically it is mainly used in treating heat toxin hair spot, unconsciousness due to high fever in veterinary drug.
Version in 2010《Republic of China Veterinary Pharmacopoeia》The quality standard of two middle qingwen baidu powders only has microscopical characters
Differentiate with the thin layer of the coptis, it is difficult to control its quality, ensure the security, validity and quality controllability of product.But existing skill
The effect of lophatherum gracile thin-layer identification method disclosed in art is poor, it is impossible to meet demand.
The content of the invention
The technical problems to be solved by the invention are：Qingwen baidu powder lophatherum gracile thin-layer identification method is imitated in the prior art
Fruit it is poor, and it is an object of the present invention to provide in a kind of excellent qingwen baidu powder of Detection results lophatherum gracile thin-layered chromatography detection method.
The present invention is achieved through the following technical solutions：
The thin-layered chromatography detection method of lophatherum gracile, comprises the following steps in a kind of qingwen baidu powder：
（1）Need testing solution is prepared using qingwen baidu powder；
（2）Need testing solution point is drawn on silica gel thin-layer plate, using ratio as 2.2: 4.8: 2.5: 0.8: 1.5 diformazan
Benzene, ethyl acetate, acetone, formic acid and water are solvent, are deployed, and take out, dry；
（3）Put and inspected under ultraviolet lamp.
The thin layer of the lophatherum gracile differentiates that the solvent combination variety used is various in the prior art, but due in different pharmaceutical
Each composition is different, and combination and proportion of composing suitable for the lophatherum gracile thin layer discriminating solvent of other drugs are not
Can suitable for qingwen baidu powder of the present invention lophatherum gracile discriminating, and in the prior art be used for qingwen baidu powder in lophatherum gracile reflect
Other solvent expansion effect is not fine.
Need testing solution is deployed by the solvent of said ratio, its expanding performance is excellent, other in qingwen baidu powder
Constituent on lophatherum gracile without influence, and by Chromatogram display, using the method for the present invention, in the chromatogram of need testing solution
Spot separating degree is splendid, the position corresponding with the chromatogram of control medicinal material solution, the Chromatogram display same color of need testing solution
Spot, and negative sample solution is noiseless.
Further, the preparation process of the need testing solution is as follows：
Qingwen baidu powder 2g is taken, adds methanol 10ml, ultrasonic 30min, is filtered, filtrate is evaporated, and it is to supply to add methanol 2mL dissolvings
Test sample solution.
In order to reach optimal separating effect, the silica gel thin-layer plate uses silica gel g thin-layer plate.
Preferably, the wavelength of the ultraviolet lamp is 365nm.
Further, also point has control medicinal material solution and negative sample solution on the silica gel thin-layer plate, and the control medicinal material is molten
The preparation method of liquid and negative sample solution is as follows：
Control medicinal material lophatherum gracile 0.5g is taken, adds methanol 10ml, ultrasonic 30min, is filtered, filtrate is evaporated, and adds methanol 2mL molten
Solution, as control medicinal material solution；
Configuring first does not include the qingwen baidu powder prescription of lophatherum gracile, takes the 1/500 of recipe quantity, adds methanol 10ml, ultrasound
30min, filtering, filtrate are evaporated, and add methanol 2mL to dissolve, as negative sample solution.
The present invention compared with prior art, has the following advantages and advantages：
The expanding performance of detection method is excellent, and other constituents are to lophatherum gracile Detection results in qingwen baidu powder
Without influence, and spot separating degree is splendid in the chromatogram of need testing solution, and negative sample solution is noiseless to its, and effect is very aobvious
Write.
Brief description of the drawings
Accompanying drawing described herein is used for providing further understanding the embodiment of the present invention, forms one of the application
Point, do not form the restriction to the embodiment of the present invention.In the accompanying drawings：
Fig. 1 is the thin-layer chromatogram of embodiment 1.
Fig. 2 is the thin-layer chromatogram of embodiment 2.
Embodiment
For the object, technical solutions and advantages of the present invention are more clearly understood, with reference to embodiment and accompanying drawing, to this
Invention is described in further detail, and exemplary embodiment of the invention and its explanation are only used for explaining the present invention, do not make
For limitation of the invention.
Embodiment 1
The thin-layered chromatography detection method of lophatherum gracile, comprises the following steps in a kind of qingwen baidu powder：
（1）It is utilized respectively qingwen baidu powder, lophatherum gracile and the qingwen baidu powder prescription including lophatherum gracile does not prepare test sample
Solution, control medicinal material solution and negative sample solution；Specific preparation process is as follows：
Qingwen baidu powder 2g is taken, adds methanol 10ml, ultrasonic 30min, is filtered, filtrate is evaporated, and it is to supply to add methanol 2mL dissolvings
Test sample solution；
Control medicinal material lophatherum gracile 0.5g is taken, adds methanol 10ml, ultrasonic 30min, is filtered, filtrate is evaporated, and adds methanol 2mL molten
Solution, as control medicinal material solution；
Configuring first does not include the qingwen baidu powder prescription of lophatherum gracile, takes the 1/500 of recipe quantity, adds methanol 10ml, ultrasound
30min, filtering, filtrate are evaporated, and add methanol 2mL to dissolve, as negative sample solution.
（2）Need testing solution point is drawn on silica gel thin-layer plate, using ratio as 2.2: 4.8: 2.5: 0.8: 1.5 diformazan
Benzene, ethyl acetate, acetone, formic acid and water are solvent, are deployed, and take out, dry.
（3）It is placed under the ultraviolet lamp that wavelength is 365nm and inspects.
The chromatographic results that show after drying as shown in figure 1, be followed successively by from left to right control medicinal material solution, need testing solution,
And negative sample solution, control medicinal material solution and negative sample solution are two, and need testing solution is one.Pass through Fig. 1
As can be seen that opening position corresponding with control medicinal material solution chromatogram on need testing solution shows red spot, negative sample is molten
Spot of the chromatogram corresponding position without same color of liquid, represent that the negative sample solution is noiseless.
Embodiment 2
The present embodiment is the comparative examples of embodiment 1, and the proportion of composing of the solvent is different in the present embodiment, specifically such as
Under：
Solvent be ratio be 2.2: 4.8: 2.5: 0.8: 0.6 dimethylbenzene, ethyl acetate, acetone, formic acid, He Shui.
When being handled using the solvent of the present embodiment ratio, as shown in Fig. 2 test sample chromatogram and negative sample chromatogram one
Cause, negative serious interference.
Above-described embodiment, the purpose of the present invention, technical scheme and beneficial effect are carried out further
Describe in detail, should be understood that the embodiment that the foregoing is only the present invention, be not intended to limit the present invention
Protection domain, within the spirit and principles of the invention, any modification, equivalent substitution and improvements done etc., all should include
Within protection scope of the present invention.
Claims (4)
1. the thin-layered chromatography detection method of lophatherum gracile in a kind of qingwen baidu powder, it is characterised in that comprise the following steps：
(1) need testing solution is prepared using qingwen baidu powder；Wherein, the preparation process of the need testing solution is as follows：Take clear pest
Baidu powder 2g, add methanol 10ml, ultrasonic 30min, filter, filtrate is evaporated, and it is need testing solution to add methanol 2mL dissolvings；
(2) need testing solution point is drawn on silica gel thin-layer plate, dimethylbenzene, second using ratio as 2.2: 4.8: 2.5: 0.8: 1.5
Acetoacetic ester, acetone, formic acid and water are solvent, are deployed, and take out, dry；
(3) put and inspected under ultraviolet lamp.
2. the thin-layered chromatography detection method of lophatherum gracile in a kind of qingwen baidu powder according to claim 1, it is characterised in that
The silica gel thin-layer plate uses silica gel g thin-layer plate.
3. the thin-layered chromatography detection method of lophatherum gracile in a kind of qingwen baidu powder according to claim 2, it is characterised in that
The wavelength of the ultraviolet lamp is 365nm.
4. the thin-layered chromatography detection method of lophatherum gracile in a kind of qingwen baidu powder according to claim 3, it is characterised in that
Also point has control medicinal material solution and negative sample solution on the silica gel thin-layer plate, the control medicinal material solution and negative sample solution
Preparation method it is as follows：
Control medicinal material lophatherum gracile 0.5g is taken, adds methanol 10ml, ultrasonic 30min, is filtered, filtrate is evaporated, and adds methanol 2mL to dissolve, i.e.,
For control medicinal material solution；
Configuring first does not include the qingwen baidu powder prescription of lophatherum gracile, takes the 1/500 of recipe quantity, adds methanol 10ml, ultrasound
30min, filtering, filtrate are evaporated, and add methanol 2mL to dissolve, as negative sample solution.
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| CN102698116B (en) * | 2012-07-03 | 2014-06-04 | 南京中医药大学 | Application of lophatherum gracile total flavonoids in preparation of medicine and functional food for preventing and treating myocardial ischemia disease |
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