CN105738553A - Thin-layer chromatography detection method for lophatherum gracile in scourge-clearing toxin-vanquishing powder - Google Patents
Thin-layer chromatography detection method for lophatherum gracile in scourge-clearing toxin-vanquishing powder Download PDFInfo
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- CN105738553A CN105738553A CN201610080410.0A CN201610080410A CN105738553A CN 105738553 A CN105738553 A CN 105738553A CN 201610080410 A CN201610080410 A CN 201610080410A CN 105738553 A CN105738553 A CN 105738553A
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
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Abstract
The invention discloses a thin-layer chromatography detection method for lophatherum gracile in scourge-clearing toxin-vanquishing powder.The problem that the effect of an existing thin-layer authentication method for lophatherum gracile in scourge-clearing toxin-vanquishing powder is poor is solved.The method comprises the steps that 1, the scourge-clearing toxin-vanquishing powder is utilized for preparing a solution for test articles; 2, the solution for test articles is sucked to be dispensed on a silica gel thin-layer plate, xylene, ethyl acetate, acetone, formic acid and water with the ratio of 2.2:4.8:2.5:0.8:1.5 are adopted as a development solvent, and the development agent is developed, taken out and dried in air; 3, the ultraviolet lamp is placed under an ultraviolet lamp to be viewed.The thin-layer chromatography detection method has the advantages that the development performance is excellent, and other ingredients in the thin-layer chromatography detection method have no effect on the lophatherum gracile detection effect.
Description
Technical field
The present invention relates to a kind of detection method, be specifically related in a kind of antipyretic and antitoxic powder the thin-layered chromatography detection method of Herba Lophatheri.
Background technology
Antipyretic and antitoxic powder is the recipe that teacher is stupid formulated more than the Qing Dynasty famous epidemic febrile disease scholar, is loaded in its " A View of Epidemic Febrile Diseases with Rashes " book shown, is now embodied in " People's Republic of China's veterinary drug allusion quotation " version two in 2010.This medicine is made up of 14 taste medical materials such as Gypsum Fibrosum, Radix Rehmanniae, Cornu Bubali, Rhizoma Coptidis, is " Baihu Tang ", " Rhizoma Coptidis toxic materials clearing away decoction " and " xijiao Dihuang Tang " tripartite's addition or subtraction of changes and obtains.The prescription of antipyretic and antitoxic powder includes: Gypsum Fibrosum 120g, Radix Rehmanniae 30g, Cornu Bubali 60g, Rhizoma Coptidis 20g, Fructus Gardeniae 30g, Cortex Moutan 20g, Radix Scutellariae 25g, Radix Paeoniae Rubra 25g, Radix Scrophulariae 25g, Rhizoma Anemarrhenae 30g, Fructus Forsythiae 30g, Radix Platycodonis 25g, Radix Glycyrrhizae 15g, Herba Lophatheri 25g；There is eliminating fire and detoxication, effect of removing heat from blood, it is mainly used in treatment pyretic toxicity clinically at veterinary drug and sends out speckle, unconsciousness due to high fever.
The quality standard of version " People's Republic of China's veterinary drug allusion quotation " two middle antipyretic and antitoxic powder in 2010 only has the thin layer of microscopical identification and Rhizoma Coptidis and differentiates, is difficult to control its quality, it is ensured that the safety of product, effectiveness and quality controllability.But the effect of the Herba Lophatheri thin-layer identification method disclosed in prior art is poor, it is impossible to meet demand.
Summary of the invention
The technical problem to be solved is: in prior art, antipyretic and antitoxic powder is poor by Herba Lophatheri thin-layer identification method effect, it is therefore intended that provide the thin-layered chromatography detection method of Herba Lophatheri in a kind of antipyretic and antitoxic powder of Detection results excellence.
The present invention is achieved through the following technical solutions:
The thin-layered chromatography detection method of Herba Lophatheri in a kind of antipyretic and antitoxic powder, comprises the following steps:
(1) antipyretic and antitoxic powder is utilized to prepare need testing solution；
(2) draw need testing solution point on silica gel thin-layer plate, with ratio be 2.2: 4.8: 2.5: 0.8: 1.5 dimethylbenzene, ethyl acetate, acetone, formic acid and water for developing solvent, launch, take out, dry；
(3) put and inspect under ultra-violet lamp.
In prior art, the thin layer of this Herba Lophatheri differentiates that the developing solvent combination variety adopted is various, but owing in different pharmaceutical, each composition is different, suitable in the compound mode of Herba Lophatheri thin layer discriminating developing solvent of other drug and proportion of composing can not the discriminating of Herba Lophatheri suitable in antipyretic and antitoxic powder of the present invention, and the developing solvent differentiated for Herba Lophatheri in antipyretic and antitoxic powder in prior art to launch effect be not fine.
By the developing solvent of said ratio, need testing solution is launched, its expanding performance is excellent, in antipyretic and antitoxic powder other constituents on Herba Lophatheri without impact, and by Chromatogram display, the method adopting the present invention, in the chromatograph of need testing solution, speckle separating degree is splendid, the position corresponding with the chromatograph of control medicinal material solution, the speckle of the Chromatogram display same color of need testing solution, and negative sample solution is noiseless.
Further, the preparation process of described need testing solution is as follows:
Taking antipyretic and antitoxic powder 2g, add methanol 10ml, ultrasonic 30min, filter, filtrate is evaporated, and adds methanol 2mL dissolving and is need testing solution.
In order to reach the separating effect of the best, described silica gel thin-layer plate adopts silica gel g thin-layer plate.
Preferably, the wavelength of described ultra-violet lamp is 365nm.
Further, described silica gel thin-layer plate also having control medicinal material solution and negative sample solution, this control medicinal material solution is as follows with the preparation method of negative sample solution:
Taking control medicinal material Herba Lophatheri 0.5g, add methanol 10ml, ultrasonic 30min, filter, filtrate is evaporated, and adds methanol 2mL and dissolves, is control medicinal material solution；
First configuring the antipyretic and antitoxic powder prescription not including Herba Lophatheri, take the 1/500 of recipe quantity, add methanol 10ml, ultrasonic 30min, filter, filtrate is evaporated, and adds methanol 2mL and dissolves, is negative sample solution.
The present invention compared with prior art, has such advantages as and beneficial effect:
The expanding performance of detection method is excellent, and in antipyretic and antitoxic powder, other constituents are on Herba Lophatheri Detection results without impact, and in the chromatograph of need testing solution, speckle separating degree is splendid, and negative sample solution is noiseless to it, effect highly significant.
Accompanying drawing explanation
Accompanying drawing described herein is used for providing being further appreciated by the embodiment of the present invention, constitutes the part of the application, is not intended that the restriction to the embodiment of the present invention.In the accompanying drawings:
Fig. 1 is the thin-layer chromatogram of embodiment 1.
Fig. 2 is the thin-layer chromatogram of embodiment 2.
Detailed description of the invention
For making the object, technical solutions and advantages of the present invention clearly understand, below in conjunction with embodiment and accompanying drawing, the present invention is described in further detail, and exemplary embodiment and the explanation thereof of the present invention are only used for explaining the present invention, not as a limitation of the invention.
Embodiment 1
The thin-layered chromatography detection method of Herba Lophatheri in a kind of antipyretic and antitoxic powder, comprises the following steps:
(1) it is utilized respectively antipyretic and antitoxic powder, Herba Lophatheri and does not include the antipyretic and antitoxic powder formula preparation need testing solution of Herba Lophatheri, control medicinal material solution and negative sample solution；Concrete preparation process is as follows:
Taking antipyretic and antitoxic powder 2g, add methanol 10ml, ultrasonic 30min, filter, filtrate is evaporated, and adds methanol 2mL dissolving and is need testing solution；
Taking control medicinal material Herba Lophatheri 0.5g, add methanol 10ml, ultrasonic 30min, filter, filtrate is evaporated, and adds methanol 2mL and dissolves, is control medicinal material solution；
First configuring the antipyretic and antitoxic powder prescription not including Herba Lophatheri, take the 1/500 of recipe quantity, add methanol 10ml, ultrasonic 30min, filter, filtrate is evaporated, and adds methanol 2mL and dissolves, is negative sample solution.
(2) draw need testing solution point on silica gel thin-layer plate, with ratio be 2.2: 4.8: 2.5: 0.8: 1.5 dimethylbenzene, ethyl acetate, acetone, formic acid and water for developing solvent, launch, take out, dry.
(3) it is placed under the ultra-violet lamp that wavelength is 365nm to inspect.
The chromatographic results manifested after drying is two as it is shown in figure 1, be followed successively by control medicinal material solution, need testing solution and negative sample solution, control medicinal material solution and negative sample solution from left to right, and need testing solution is one.As seen in Figure 1, the aobvious red speckle in position corresponding with control medicinal material solution chromatograph on need testing solution, the chromatograph corresponding position of negative sample solution, without the speckle of same color, represents that this negative sample solution is noiseless.
Embodiment 2
The present embodiment is the comparative examples of embodiment 1, and the proportion of composing of this developing solvent in the present embodiment is different, specific as follows:
Developing solvent is ratio be 2.2: 4.8: 2.5: 0.8: 0.6 dimethylbenzene, ethyl acetate, acetone, formic acid, He Shui.
When the developing solvent adopting the present embodiment ratio processes, as in figure 2 it is shown, test sample chromatograph is consistent with negative sample chromatograph, negative serious interference.
Above-described detailed description of the invention; the purpose of the present invention, technical scheme and beneficial effect have been further described; it is it should be understood that; the foregoing is only the specific embodiment of the present invention; the protection domain being not intended to limit the present invention; all within the spirit and principles in the present invention, any amendment of making, equivalent replacement, improvement etc., should be included within protection scope of the present invention.
Claims (5)
1. the thin-layered chromatography detection method of Herba Lophatheri in an antipyretic and antitoxic powder, it is characterised in that comprise the following steps:
(1) antipyretic and antitoxic powder is utilized to prepare need testing solution；
(2) draw need testing solution point on silica gel thin-layer plate, with ratio be 2.2: 4.8: 2.5: 0.8: 1.5 dimethylbenzene, ethyl acetate, acetone, formic acid and water for developing solvent, launch, take out, dry；
(3) put and inspect under ultra-violet lamp.
2. the thin-layered chromatography detection method of Herba Lophatheri in a kind of antipyretic and antitoxic powder according to claim 1, it is characterised in that the preparation process of described need testing solution is as follows:
Taking antipyretic and antitoxic powder 2g, add methanol 10ml, ultrasonic 30min, filter, filtrate is evaporated, and adds methanol 2mL dissolving and is need testing solution.
3. the thin-layered chromatography detection method of Herba Lophatheri in a kind of antipyretic and antitoxic powder according to claim 1 and 2, it is characterised in that described silica gel thin-layer plate adopts silica gel g thin-layer plate.
4. the thin-layered chromatography detection method of Herba Lophatheri in a kind of antipyretic and antitoxic powder according to claim 3, it is characterised in that the wavelength of described ultra-violet lamp is 365nm.
5. the thin-layered chromatography detection method of Herba Lophatheri in a kind of antipyretic and antitoxic powder according to claim 4, it is characterized in that, also having control medicinal material solution and negative sample solution on described silica gel thin-layer plate, this control medicinal material solution is as follows with the preparation method of negative sample solution:
Taking control medicinal material Herba Lophatheri 0.5g, add methanol 10ml, ultrasonic 30min, filter, filtrate is evaporated, and adds methanol 2mL and dissolves, is control medicinal material solution；
First configuring the antipyretic and antitoxic powder prescription not including Herba Lophatheri, take the 1/500 of recipe quantity, add methanol 10ml, ultrasonic 30min, filter, filtrate is evaporated, and adds methanol 2mL and dissolves, is negative sample solution.
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| CN107247111A (en) * | 2017-06-15 | 2017-10-13 | 江西傲新生物科技有限公司 | The thin-layered chromatography detection method of lophatherum gracile in a kind of Yinqiao San |
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| CN107247111A (en) * | 2017-06-15 | 2017-10-13 | 江西傲新生物科技有限公司 | The thin-layered chromatography detection method of lophatherum gracile in a kind of Yinqiao San |
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