CN106770884A - The thin-layered chromatography detection method of Radix Angelicae Sinensis in a kind of female biochemistry mixture of benefit - Google Patents
The thin-layered chromatography detection method of Radix Angelicae Sinensis in a kind of female biochemistry mixture of benefit Download PDFInfo
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- CN106770884A CN106770884A CN201710123835.XA CN201710123835A CN106770884A CN 106770884 A CN106770884 A CN 106770884A CN 201710123835 A CN201710123835 A CN 201710123835A CN 106770884 A CN106770884 A CN 106770884A
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- radix angelicae
- angelicae sinensis
- benefit
- biochemistry mixture
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/90—Plate chromatography, e.g. thin layer or paper chromatography
Abstract
The invention discloses the thin-layered chromatography detection method of Radix Angelicae Sinensis in a kind of female biochemistry mixture of benefit；Solve and there are problems that in the prior art that development rate is slack-off, spot is unsharp.Preparation of the present invention including (1) need testing solution：Take beneficial female biochemistry mixture and be made need testing solution；(2) need testing solution point being drawn on silica gel thin-layer plate, being 4: 1 n-hexane and ethyl acetate as solvent with ratio, expansion, taking-up is dried；(3) it is placed under ultraviolet lamp and inspects.The present invention can effectively improve the effect for launching efficiency and expansion, and effect is very notable.
Description
Technical field
The present invention relates to a kind of thin-layered chromatography detection method, and in particular to the thin layer color of Radix Angelicae Sinensis in a kind of female biochemistry mixture of benefit
Spectrum detection method.
Background technology
Beneficial female biochemistry mixture by motherwort, Radix Angelicae Sinensis, Ligusticum wallichii, peach kernel, baked ginger, Radix Glycyrrhizae (processing) above Six-element medicinal material processing and
Into.It is greenish orange yellow to brown color liquid.Gas is fragrant, and taste is micro- sweet.With activating blood and removing stasis, the function such as antalgic.Cure mainly postpartum
Lochia, abdominal pain due to blood stasis.The female biochemistry mixture of the benefit is mainly used in the livestocks such as horse, ox, sheep, pig, and wherein horse, ox consumption is 200-
300mL, sheep, pig consumption are 30-50mL.
Current said preparation record in《Veterinary drug national standard collects --- and veterinary drug provincial standard rises national standard》(second
Volume), indentification by TLC only is carried out to motherwort, Radix Glycyrrhizae, Ligusticum wallichii in its quality standard, without content measuring standard, it is difficult to control
Make its quality, it is ensured that the security of product, validity and quality controllability.
It is 5 generally with ratio in the prior art for the thin-layer chromatography detection of Radix Angelicae Sinensis:1 n-hexane-ethyl acetate
Used as solvent, it is slack-off to there is development rate, the unsharp problem of spot.
The content of the invention
The technical problems to be solved by the invention are：Exist that development rate is slack-off, spot is unsharp in the prior art
Problem, it is therefore intended that the thin-layered chromatography detection method of Radix Angelicae Sinensis in the female biochemistry mixture of a kind of benefit for solving the above problems is provided.
The present invention is achieved through the following technical solutions：
The thin-layered chromatography detection method of Radix Angelicae Sinensis in a kind of female biochemistry mixture of benefit, including：
(1) preparation of need testing solution：Take beneficial female biochemistry mixture and be made need testing solution；
(2) need testing solution point is drawn on silica gel thin-layer plate, is expansion by 4: 1 n-hexane and ethyl acetate of ratio
Agent, launches, and takes out, and dries；
(3) it is placed under ultraviolet lamp and inspects.
The optimization of the ratio that the present invention passes through solvent, effectively improves the effect for launching efficiency and expansion, makes expansion speed
Degree increases and makes the spot after expansion relatively sharp, effect is significant.
Further, the silica gel thin-layer plate uses silica gel g thin-layer plate.The wavelength of the ultraviolet lamp is 365nm.
Also point has reference substance solution and negative sample solution on the silica gel thin-layer plate, wherein, the negative sample solution is
The liquid that the female biochemistry mixture prescription medicinal material of benefit in addition to Radix Angelicae Sinensis is made according to beneficial female biochemistry mixture preparation technology；The reference substance is molten
Liquid is the liquid being made according to beneficial female biochemistry mixture preparation technology using Radix Angelicae Sinensis.
In the prior art when the thin-layer chromatography for carrying out Radix Angelicae Sinensis is detected, due to there are other compositions, other compositions in medicine
Effect can be interfered to composition to be measured, thus in different medicines, although composition to be measured is the same, but suitable for one of which
The preprocessing means of medicine are not necessarily suitable another medicine.Also, inventor has found the medicine of same materials composition, its system
When standby technique has differences, extreme influence will also result in final finished, especially for using thin-layer chromatography detection wherein certain
During one component, testing result very different, the testing result that above-mentioned factor also results in thin-layer chromatography detection is not ideal enough.
Selection by optimizing reference substance solution and negative sample solution of the invention, effectively increases detection accuracy.
The present invention compared with prior art, has the following advantages and advantages：
By optimization design of the invention, development rate can be effectively improved, and make testing result more accurate.
Brief description of the drawings
Fig. 1 is the thin-layer chromatography detection figure of embodiment 1.
Fig. 2 is the thin-layer chromatography detection figure of embodiment 2.
Specific embodiment
To make the object, technical solutions and advantages of the present invention become more apparent, with reference to embodiment, the present invention is made
Further to describe in detail, exemplary embodiment of the invention and its explanation are only used for explaining the present invention, are not intended as to this
The restriction of invention.
Embodiment 1
A kind of thin-layered chromatography detection method of Radix Angelicae Sinensis in female biochemistry mixture of benefit, specific detection process is as follows：
Take beneficial female biochemistry mixture appropriate as need testing solution；1/20 (except the Radix Angelicae Sinensis) for taking prescription medicinal material amount is female raw by benefit
Bonding agent preparation technology is made negative control solution.The another Radix Angelicae Sinensis control medicinal material that takes is appropriate, by beneficial female biochemistry mixture preparation technology system
Into reference substance solution.Draw above-mentioned each 10 μ L of three kinds of solution respectively, put respectively on same silica gel g thin-layer plate, with n-hexane and
Ethyl acetate is used as solvent, wherein n-hexane:Ethyl acetate=4:1, launch, take out, dry, put the purple under the conditions of 365nm
Inspected under outer smooth lamp.In test sample chromatogram, on position corresponding with control medicinal material chromatogram, show the fluorescent spot of same color
Point, it is negative noiseless, as a result see Fig. 1.In Fig. 1, marked as 1,2 is negative control solution, right for Radix Angelicae Sinensis marked as 3,4
According to product solution, marked as 5,6 is need testing solution.
Testing result：
The chromatographic results control medicinal material solution and the Chromatogram display clear spot of need testing solution manifested after drying, test sample
On solution at the position corresponding with reference substance solution chromatogram, the spot of the Chromatogram display same color of need testing solution, and it is cloudy
Corresponding position in the chromatogram of property sample solution does not have the spot of same color, represents that the negative sample solution is noiseless.
Embodiment 2
The present embodiment is the comparative examples of embodiment 1, and the specific ratio of solvent is different in the present embodiment, specific detection
Process is as follows：
Take beneficial female biochemistry mixture appropriate as need testing solution；1/20 (except the Radix Angelicae Sinensis) for taking prescription medicinal material amount is female raw by benefit
Bonding agent preparation technology is made negative control solution.The another Radix Angelicae Sinensis control medicinal material that takes is appropriate, by beneficial female biochemistry mixture preparation technology system
Into reference substance solution.Draw above-mentioned each 10 μ L of three kinds of solution respectively, put respectively on same silica gel g thin-layer plate, with n-hexane and
Ethyl acetate is used as solvent, wherein n-hexane:Ethyl acetate=5:1, launch, take out, dry, put the purple under the conditions of 365nm
Inspected under outer smooth lamp.In test sample chromatogram, on position corresponding with control medicinal material chromatogram, show the fluorescent spot of same color
Point, it is negative noiseless, as a result see Fig. 2.In Fig. 2, marked as 1,2 is negative control solution, right for Radix Angelicae Sinensis marked as 3,4
According to product solution, marked as 5,6 is need testing solution.
Testing result：
The chromatographic results that need testing solution manifests after drying compare negative control solution and reference substance solution without significant difference,
Testing result is extremely inaccurate.Also, in the present embodiment for embodiment 1, the development rate of solution slow down 1/3,
Greatly slow down operating efficiency.
Above-described specific embodiment, has been carried out further to the purpose of the present invention, technical scheme and beneficial effect
Describe in detail, should be understood that and the foregoing is only specific embodiment of the invention, be not intended to limit the present invention
Protection domain, all any modification, equivalent substitution and improvements within the spirit and principles in the present invention, done etc. all should include
Within protection scope of the present invention.
Claims (4)
1. in a kind of female biochemistry mixture of benefit Radix Angelicae Sinensis thin-layered chromatography detection method, it is characterised in that including：
(1) preparation of need testing solution：Take beneficial female biochemistry mixture and be made need testing solution；
(2) draw need testing solution point on silica gel thin-layer plate, the n-hexane and ethyl acetate with ratio as 4: 1 as solvent,
Launch, take out, dry；
(3) it is placed under ultraviolet lamp and inspects.
2. in the female biochemistry mixture of a kind of benefit according to claim 1 Radix Angelicae Sinensis thin-layered chromatography detection method, it is characterised in that
The silica gel thin-layer plate uses silica gel g thin-layer plate.
3. in the female biochemistry mixture of a kind of benefit according to claim 1 and 2 Radix Angelicae Sinensis thin-layered chromatography detection method, its feature exists
In the wavelength of the ultraviolet lamp is 365nm.
4. in the female biochemistry mixture of a kind of benefit according to claim 3 Radix Angelicae Sinensis thin-layered chromatography detection method, it is characterised in that
Also point has reference substance solution and negative sample solution on the silica gel thin-layer plate, wherein, the negative sample solution be except Radix Angelicae Sinensis with
The liquid that the female biochemistry mixture prescription medicinal material of outer benefit is made according to beneficial female biochemistry mixture preparation technology；The reference substance solution is use
The liquid that Radix Angelicae Sinensis is made according to beneficial female biochemistry mixture preparation technology.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710123835.XA CN106770884A (en) | 2017-03-03 | 2017-03-03 | The thin-layered chromatography detection method of Radix Angelicae Sinensis in a kind of female biochemistry mixture of benefit |
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| CN201710123835.XA CN106770884A (en) | 2017-03-03 | 2017-03-03 | The thin-layered chromatography detection method of Radix Angelicae Sinensis in a kind of female biochemistry mixture of benefit |
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| CN201710123835.XA Pending CN106770884A (en) | 2017-03-03 | 2017-03-03 | The thin-layered chromatography detection method of Radix Angelicae Sinensis in a kind of female biochemistry mixture of benefit |
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Citations (7)
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| CN1785220A (en) * | 2005-12-09 | 2006-06-14 | 贵州益佰制药股份有限公司 | Method for quality control of blood-bonifying Chinese angelica prepn. |
| CN1814126A (en) * | 2005-11-25 | 2006-08-09 | 毛晓敏 | Compound black-bone chicken soft capsule, its preparing method and quality control method |
| CN101229323A (en) * | 2008-01-21 | 2008-07-30 | 邯郸制药有限公司 | Lily oral liquid and quality standard and test method of pharmaceutical preparation thereof |
| US20090068291A1 (en) * | 2001-03-02 | 2009-03-12 | Biopharmacopae Design International Inc. | Plant extracts and compositions comprising extracellular protease inhibitors |
| CN102552800A (en) * | 2010-12-29 | 2012-07-11 | 江西济民可信集团有限公司 | Detection method of liver-strengthening syrup Chinese medicinal preparation |
| CN102879516A (en) * | 2012-09-25 | 2013-01-16 | 南京中医药大学 | Method for identifying Buyang Huanwu soup and measuring content of Buyang Huanwu soup |
| CN104280507A (en) * | 2014-09-22 | 2015-01-14 | 江苏七○七天然制药有限公司 | Thin-layer chromatographic identification method of wine for treating arthralgia and myalgia |
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2017
- 2017-03-03 CN CN201710123835.XA patent/CN106770884A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090068291A1 (en) * | 2001-03-02 | 2009-03-12 | Biopharmacopae Design International Inc. | Plant extracts and compositions comprising extracellular protease inhibitors |
| CN1814126A (en) * | 2005-11-25 | 2006-08-09 | 毛晓敏 | Compound black-bone chicken soft capsule, its preparing method and quality control method |
| CN1785220A (en) * | 2005-12-09 | 2006-06-14 | 贵州益佰制药股份有限公司 | Method for quality control of blood-bonifying Chinese angelica prepn. |
| CN101229323A (en) * | 2008-01-21 | 2008-07-30 | 邯郸制药有限公司 | Lily oral liquid and quality standard and test method of pharmaceutical preparation thereof |
| CN102552800A (en) * | 2010-12-29 | 2012-07-11 | 江西济民可信集团有限公司 | Detection method of liver-strengthening syrup Chinese medicinal preparation |
| CN102879516A (en) * | 2012-09-25 | 2013-01-16 | 南京中医药大学 | Method for identifying Buyang Huanwu soup and measuring content of Buyang Huanwu soup |
| CN104280507A (en) * | 2014-09-22 | 2015-01-14 | 江苏七○七天然制药有限公司 | Thin-layer chromatographic identification method of wine for treating arthralgia and myalgia |
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| 吕双方 等: "生化汤口服液的制备及质量控制", 《中国药业》 * |
| 赵卫国: "当归红花酊质量标准研讨", 《中国药物与临床》 * |
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Application publication date: 20170531 |