CN106834185A - A kind of Alpine-arctic Pastoral tall oat grass silage inoculants bacteria - Google Patents
A kind of Alpine-arctic Pastoral tall oat grass silage inoculants bacteria Download PDFInfo
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- CN106834185A CN106834185A CN201710126819.6A CN201710126819A CN106834185A CN 106834185 A CN106834185 A CN 106834185A CN 201710126819 A CN201710126819 A CN 201710126819A CN 106834185 A CN106834185 A CN 106834185A
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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Abstract
The invention discloses a kind of Alpine-arctic Pastoral tall oat grass silage inoculants bacteria, mixed by following raw material：Pediococcus pentosaceus 4 × 109~5 × 109CFU/g, Lactobacillus plantarum is 2 × 109~3 × 109CFU/g, the 0.6g/g of starch 0.5 and mineral matter element (0.02~0.04mg/g copper sulphate, 0.06~0.08mg/g magnesium sulfate, 0.8~1.2mg/g zinc sulfate, 6.2~6.6mg/g sodium acetates, 0.8~1.2mg/g sodium citrates).The present invention separates the ensiling lactobacillus inoculation for adapting to local environmental conditions from the oat herbage of this area's artificial growth and soil, and carry out the compounding and applicating evaluating of ensiling bacterial strain, develop the silage inoculants bacteria of the suitable feeding processing of Alpine-arctic Pastoral tall oat grass, the technical need of quality silage modulation processing is solved, artificial forage grass resource Efficient Conversion is effectively improved and is utilized technical merit.
Description
Technical field
The present invention relates to technical field of agricultural breeding, and in particular to a kind of Alpine-arctic Pastoral tall oat grass silage inoculants bacteria.
Background technology
Qinghai-xizang Plateau Region animal husbandry have substantially with it is interior regional entirely different the characteristics of, due to weather, agricultural development
Etc. reason, Structure Dynamics In The Qinghai-tibet Region animal husbandry winter still based on herding but very long brings serious subalimentation to the livestock that survives the winter
Problem, surviving the winter can cause livestock body weight loss 20~30% every year on average, itself main reason is that feed nutrition supply season
Section is uneven, in face of Qinghai-Tibet region it is wider herd the mode of production, reference concentrates cultivation interiorly, use and modernize concentrated feed
Deng industrialization management mode due to the reasons such as cost and tradition for the moment also be difficult to realize.Ensilage technology is existing several in China
10 years developing histories, are of high nutritive value because ensilage has, and simple production process, cost is relatively low, is obtained extensively in China
Using and development, especially Qinghai-Tibet Platean due to very cold, Wintering Period it is long, therefore ensilage is particularly suitable for these areas and pushes away
Wide application, simple and feasible method is exactly to increase the development of ensilage technology, is gathered materials on the spot, and improves overwintering feed trophic component,
So as to provide material guarantee to improve livestock nutrition condition.But it is special due to this area " High aititude, cold, anoxic, arid "
Climatic characteristic, causes the application effect of domestic and international silage inoculants bacteria product undesirable, the traditional ensilage skill for still using at present
Art is relatively very original, and simple aerobic fermentation and anaerobic fermentation are carried out just with original natural microbial, and its is backward in technique, produces
The speed of growth of lactic acid bacteria is relatively slow, it is difficult to plays the early stage inhibitory action to being harmful to miscellaneous bacteria, causes forage grass in ensilage
Itself nutrient is larger because living contaminants loses, and is unfavorable for that the Efficient Conversion of regional artificial forage grass resource is utilized, and nutrition composition is effective
Preserve and lifted and be limited.
The content of the invention
To solve the above problems, the invention provides a kind of Alpine-arctic Pastoral tall oat grass silage inoculants bacteria, low cost, application effect
Good tall oat grass silage inoculants bacteria, to produce the tall oat grass ensilage of high-quality, the artificial forage grass processing in lifting Qinghai Alpine Grassland
Technical merit.
To achieve the above object, the technical scheme taken of the present invention is：
A kind of Alpine-arctic Pastoral tall oat grass silage inoculants bacteria, is mixed by following raw material：
Pediococcus pentosaceus 4 × 109~5 × 109CFU/g, Lactobacillus plantarum is 2 × 109~3 × 109CFU/g, starch 0.5
~0.6g/g and mineral matter element.
Wherein, the microbial inoculum includes containing mineral matter element：
0.02~0.04mg/g copper sulphate, 0.06~0.08mg/g magnesium sulfate, 0.8~1.2mg/g zinc sulfate, 6.2~
6.6mg/g sodium acetates, 0.8~1.2mg/g sodium citrates.
The invention has the advantages that：
The ensiling breast for adapting to local environmental conditions is separated from the oat herbage of this area's artificial growth and soil
Sour strain, and the compounding and applicating evaluating of ensiling bacterial strain are carried out, develop the ensiling of the suitable feeding processing of Alpine-arctic Pastoral tall oat grass
Microbial inoculum, solves the technical need of quality silage modulation processing, effectively improves artificial forage grass resource Efficient Conversion and utilizes
Technical merit.
Specific embodiment
In order that objects and advantages of the present invention become more apparent, the present invention is carried out further with reference to embodiments
Describe in detail.It should be appreciated that specific embodiment described herein is only used to explain the present invention, it is not intended to limit the present invention.
Embodiment 1
Ensiling lactic fermentation strain
Ensiling lactic acid bacteria
Forage grass ensiling test result
(1) change of pH value
Addition lactic acid bacteria group was reduced to less than 4.0 speed in 30 days pH value, especially showed before ensiling 15 days more
It is in this way, this is to ensure the key whether good forage ensiling is successful；Meanwhile, the pH value at comprehensive difference ensiling time point is come
See, HT4 and GC10 groups are performed better than in terms of grass silage pH value regulating effect and stability.
The pH value of ensilage
Note：CK is the control group for being not added with bacterium.
(2) change of gross protein value
It is smaller that same addition lactic acid bacteria group is presented an amplitude in different ensiling time points (7d, 15d, 30d, 60d, 90d)
Fluctuation, and grass silage fermentation it is basicly stable when (after 60d) show as HT4, GC10 group and have compared with herbage former state state (0d)
It is certain to improve, and control group decreases.
The content (%) of thick protein
Note：The identical lowercase letter mean difference of same column is not notable (p ＞ 0.05) in table, same column difference lowercase
Represent mean difference significantly (p ＜ 0.05).Similarly hereinafter.
(3) change of acid detergent fiber (ADF)
With ensiling time lengthening acid detergent fiber content in the variation tendency that first rising is reduced afterwards (before 15d), meanwhile, when
Grass silage fermentation enter the basicly stable phase (after 60d) show as addition lactic acid bacteria group and control group herbage ADF contents compared with
(0d) has relatively low to a certain degree as former state for ensiling.
Acid detergent fiber content (%)
(4) change of In vitro digestibility (IVOMD)
When grass silage enter stationary phase (after 60 days), the IVOMD of each group ensiling herbage between 70%~72.3%, and
The IVOMD of each group increases compared with former state state (0d) before ensiling, shown as after 60 days addition lactic acid bacteria group be above it is right
According to group.
In Vitro gas production method estimates organic matter digestibility (%)
(5) change of metabolizable energy (ME)
When grass silage enters stationary phase (after 60 days), the ME values scope of each group ensiling herbage 9~11MJ/kgDM it
Between, and the ME of each group increases compared with former state state (0d) before ensiling, and addition lactic acid bacteria group is slightly higher and control group, but respectively
Between group without significant difference (P ＞ 0.05), show that the metabolizable energy after grass silage is close with its ensiling raw material.
In Vitro gas production method estimates metabolizable energy (MJ/kg DM)
(6) change of lactic acid content
With in the extension ensilage of ensiling time, lactic acid content slightly has reduction and tends towards stability in first rising afterwards on the whole
Change, i.e. basically reach peak before 15d, and the lactic acid content early stage (before 15d) of addition lactic acid bacteria group is higher than control group,
It is declined slightly afterwards and tends towards stability, each group lactic acid content is substantially close.Indicating addition ensiling lactic acid bacteria can promote herbage blue or green
Storage fermentation, and then quick raising fermentation lactic acid concentration, finally play and effectively suppress the unfavorable hair of corrupt miscellaneous bacteria during grass silage
The effect of ferment.
The content (DM%) of lactic acid
Embodiment 2
The composition of compound silage inoculants bacteria
The composition of compound silage inoculants bacteria
Grass silage result of the test
(1) change of pH value
PH value after grass silage
Note：CK is the control group for being not added with microbial inoculum.
Addition microbial inoculum group pH value before ensiling 70 days declines quickly and continually and steadily, and regulation grass silage is fermented the effect of pH value
Fruit is very well.
(2) change of conventional nutrient content
CP, EE and WSC have compared with control group and improve to a certain extent in nutrient content after grass silage, and ADF and NDF
Having significantly reduces, and is the good behaviour that the nutritional quality of forage grass is stored.
Conventional nutrient content (ensiling the 70th day)
(3) simulation rumen gas production in vitro fermentation evaluation
It is in notable positively related result of study according to feed gas production in vitro amount and its dry digestibility (DMD), addition is blue or green
Storage microbial inoculum can improve the dry digestibility of herbage, and microbial inoculum group improves 22% compared with control group.
In Vitro Fermentation 48h aerogenesis dynamic parameters
(4) comparing of IVOMD and ME
The ME and IVOMD for adding microbial inoculum group ensiling herbage are above control group, and compared to control group, ensiling herbage ME improves
21%, IVOMD improve 18%.It is similar that this improves trend to the dry digestibility that gas production in vitro amount is evaluated indirectly.
External organic matter digestibility (IVOMD) and metabolizable energy (ME)
The compound silage inoculants bacteria developed using the present invention can make oat herbage pH value before ensiling 70 days decline quickly and hold
Continuous stabilization, can the suitable pH value of Effective Regulation grass silage fermentation；Thick protein (CP) after grass silage, crude fat (EE) and can
Dissolubility sugar (WSC) content has compared with control group and improves to a certain extent, and acid detergent fiber (ADF) and neutral detergent fiber
(NDF) having significantly reduces.Addition microbial inoculum group compares control group, improves the dry digestibility (DMD) of herbage
22%, metabolizable energy (ME) improves 21%, and external organic matter digestibility (IVOMD) improves 18%；Meanwhile, tall oat grass ensiling
Preserved after fermentation stability using the cumulative time more than 10 months, reached this area's artificial growth herbage science processing and utilized
Produce reality demand.
When this specific implementation is used, cutting tall oat grass (milk stage is cradled) according to 1000kg green grass or young crops need to add the ratio of 5g silage inoculants bacterias
Row carry out (final moisture content control be advisable between 60%~65%), and microbial inoculum using it is preceding first must be in 37~40 DEG C of appropriate temperature
Carry out dissolving 1~2h of activation in boiling water, then by than spraying addition in the row backward tall oat grass of dilution, can guarantee that its good makes
Use effect.
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (2)
1. a kind of Alpine-arctic Pastoral tall oat grass silage inoculants bacteria, it is characterised in that mixed by following raw material：
Pediococcus pentosaceus 4 × 109~5 × 109CFU/g, Lactobacillus plantarum is 2 × 109~3 × 109CFU/g, starch 0.5-0.6g/
G and mineral matter element.
2. a kind of Alpine-arctic Pastoral tall oat grass silage inoculants bacteria as claimed in claim 1, it is characterised in that the microbial inoculum contains mineral matter
Element includes：
0.02~0.04mg/g copper sulphate, 0.06~0.08mg/g magnesium sulfate, 0.8~1.2mg/g zinc sulfate, 6.2~6.6mg/g
Sodium acetate, 0.8~1.2mg/g sodium citrates.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710126819.6A CN106834185A (en) | 2017-03-06 | 2017-03-06 | A kind of Alpine-arctic Pastoral tall oat grass silage inoculants bacteria |
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| CN201710126819.6A CN106834185A (en) | 2017-03-06 | 2017-03-06 | A kind of Alpine-arctic Pastoral tall oat grass silage inoculants bacteria |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107603923A (en) * | 2017-11-06 | 2018-01-19 | 四川省草原科学研究院 | 3 strains of lactic acid bacteria of Alpine-arctic Pastoral ensiling |
| CN108060103A (en) * | 2018-01-29 | 2018-05-22 | 四川农业大学 | A kind of Pediococcus pentosaceus and its application, screening calibration method |
| CN108265016A (en) * | 2018-01-29 | 2018-07-10 | 四川农业大学 | A kind of lactic bacteria additive and its application, preparation method |
| CN109566872A (en) * | 2019-01-15 | 2019-04-05 | 甘肃农业大学 | A kind of modulator approach of Alpine-arctic Pastoral triticale ensilage |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1409986A (en) * | 2002-06-28 | 2003-04-16 | 王建华 | Special lactobacillus inoculum for silage and its making method |
| WO2015120098A1 (en) * | 2014-02-04 | 2015-08-13 | Micro-Nature Llc | Systems, methods, and compositions relating to combiomics |
-
2017
- 2017-03-06 CN CN201710126819.6A patent/CN106834185A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1409986A (en) * | 2002-06-28 | 2003-04-16 | 王建华 | Special lactobacillus inoculum for silage and its making method |
| WO2015120098A1 (en) * | 2014-02-04 | 2015-08-13 | Micro-Nature Llc | Systems, methods, and compositions relating to combiomics |
Non-Patent Citations (2)
| Title |
|---|
| XIAOHUA NIE等: "Proteolytic characterisation in grass carp sausage inoculated with Lactobacillus plantarum and Pediococcus pentosaceus", 《FOOD CHEMISTRY》 * |
| 韩建林: "饲料青贮保存的研究进展", 《国外畜牧学》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107603923A (en) * | 2017-11-06 | 2018-01-19 | 四川省草原科学研究院 | 3 strains of lactic acid bacteria of Alpine-arctic Pastoral ensiling |
| CN108060103A (en) * | 2018-01-29 | 2018-05-22 | 四川农业大学 | A kind of Pediococcus pentosaceus and its application, screening calibration method |
| CN108265016A (en) * | 2018-01-29 | 2018-07-10 | 四川农业大学 | A kind of lactic bacteria additive and its application, preparation method |
| CN108265016B (en) * | 2018-01-29 | 2019-01-11 | 四川农业大学 | A kind of lactic bacteria additive and its application, preparation method |
| CN109566872A (en) * | 2019-01-15 | 2019-04-05 | 甘肃农业大学 | A kind of modulator approach of Alpine-arctic Pastoral triticale ensilage |
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Application publication date: 20170613 |
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