CN106834086B - A kind of separation of fermentative broth device - Google Patents

A kind of separation of fermentative broth device Download PDF

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Publication number
CN106834086B
CN106834086B CN201710098811.3A CN201710098811A CN106834086B CN 106834086 B CN106834086 B CN 106834086B CN 201710098811 A CN201710098811 A CN 201710098811A CN 106834086 B CN106834086 B CN 106834086B
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pipeline
cylinder
hole
pipe
outlet
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CN106834086A (en
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臧武波
程秋洁
彭荣达
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Zhejiang Lanbo Biological Polytron Technologies Inc
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Zhejiang Lanbo Biological Polytron Technologies Inc
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/10Separation or concentration of fermentation products
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D61/00Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/58Multistep processes

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  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Sustainable Development (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Water Supply & Treatment (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)

Abstract

The present invention provides a kind of separation of fermentative broth devices, belong to technological field of biochemistry.It solves the problems, such as that separation of fermentative broth effect is poor in the prior art.This separation of fermentative broth device, including pedestal, pedestal, which is equipped with one, can go out separation of fermentative broth the cylinder of different size particle, cylinder is secured transverse on pedestal, one end of cylinder has inlet, the other end of cylinder has liquid outlet, filter membrane one is successively arranged from inlet to liquid outlet in cylinder, filter membrane two, filter membrane three and filter membrane four, the side of the inlet of cylinder is equipped with the cartridge filter of impurity in an energy filtering fermentating liquid, cartridge filter inner wall is equipped with a stop collar, stop collar is equipped with a filter screen, the outer wall top of cartridge filter offers an import, the bottom of cartridge filter offers an outlet, the outlet of cartridge filter is connected with the inlet of cylinder by pipeline two, the import of cartridge filter is connected with one end of a pipeline one.The present invention has the advantages that repeatedly filter separation and fermentation liquid.

Description

A kind of separation of fermentative broth device
Technical field
The invention belongs to technological field of biochemistry, are related to a kind of separator, especially a kind of separation of fermentative broth device.
Background technique
The Chinese nickname of niacinamide has: Nicotinic Acid Amide, vitamin B3 (VB3), niacinamide, Niacinamide, English Entitled nicotinamide, molecular formula C6H6N2O, molecular weight 122.13 are white, needle-shaped crystals or powder, belong to vitamin B Race.The one kind of niacinamide as B family vitamin is the important component of human body and animal body cozymase and codehydrogenase Ⅱ, participates in body Inner lipid metabolism, the oxidation process of tissue respiration and the process of carbohydrate anaerobic decomposition.It is the composition of codehydrogenase in organism Part can promote biological oxidation process and tissue metabolism, to maintenance normal tissue (especially skin, alimentary canal and nerve System) integrality play a significant role.Therefore, niacinamide be widely used in food and feed additive, drug and bulk pharmaceutical chemicals, Cosmetic additive agent and biochemical agent, colour former, brightening agent etc..
The technological process of production of niacinamide is successively such that biocatalyst culture (production strain-seeding tank-fermentation Tank-UF membrane) and biocatalytic reaction and post-processing (catalysis hydration reaction-UF membrane Ultrafiltration-Concentration-granulation-drying-sieve Point);This procedure of UF membrane need to use separator, and existing separator structure is relatively easy, not reuse slag liquid, Cause fermentation liquid utilization rate low, production finished product is high.So those skilled in the art has and to be developed a kind of can make fermentation liquid repeatedly The separator of filtering, separator have the advantages that filter effect is good, high production efficiency.
Summary of the invention
The purpose of the present invention is there is the above problem in view of the prior art, a kind of separation of fermentative broth device is proposed, originally Invention has the characteristics that separation and fermentation liquid can be repeatedly filtered.
Object of the invention can be realized by the following technical scheme: a kind of separation of fermentative broth device, including pedestal, special Sign is that the pedestal, which is equipped with one, can go out separation of fermentative broth the cylinder of different size particle, and the cylinder is horizontal To being fixed on the base, one end of cylinder has an inlet, and the other end of cylinder has a liquid outlet, in the cylinder It is successively arranged filter membrane one, filter membrane two, filter membrane three and filter membrane four from inlet to liquid outlet, is opened on the outer wall of cylinder Equipped with several mud holes one, the side of cylinder is equipped with one for storing the transferring box of waste residue, sets between transferring box and cylinder There are a scum pipe and several deslagging branch pipes corresponding with mud hole one, one end of several mud holes one and several deslagging branch pipes is one by one Correspondence is connected, and one end of scum pipe is closed end, and the other end of scum pipe is outlet end, tube body of the scum pipe close to closed end On offer several mud holes two, several mud holes two are connected with the other end of several deslagging branch pipes one-to-one correspondence, transferring box With slag input hole and scum hole, the outlet end of scum pipe is connected with the slag input hole of transferring box, the side of the inlet of cylinder Cartridge filter equipped with impurity in an energy filtering fermentating liquid, cartridge filter inner wall are equipped with a stop collar, and stop collar is equipped with a filter screen, The outer wall top of cartridge filter offers an import, and the bottom of cartridge filter offers an outlet, the outlet of cartridge filter and cylinder Inlet is connected by pipeline two, and the import of cartridge filter is connected with one end of a pipeline one, and the one of the liquid outlet of cylinder Side is equipped with a fluid reservoir, is connected between fluid reservoir and the liquid outlet of cylinder by pipeline three, the other end connection of pipeline one The input port two-way piping four of the input port one of one tee tube, tee tube connects a strain holding vessel, the delivery outlet of tee tube The output end of a vacuum pump is connected by pipeline five, the input terminal of vacuum pump connects a desinfection chamber by pipeline six, pipeline one A hole for back flow is offered on tube body, the hole for back flow is also provided with a cleaning on tee tube one end, the tube body of pipeline one Hole, the cleaning hole are between the other end and hole for back flow of pipeline one, pass through between the scum hole and hole for back flow of transferring box One residue outlet communicates, and the cooling of an energy cooling and fermentation liquid is additionally provided on the tube body between the hole for back flow and one end of pipeline one Mechanism.
This separation of fermentative broth device, its working principle is that such: firstly, strain is stored in strain storage after the fermentation has been completed It deposits in tank, strain holding vessel is placed on relatively high position, and the fermentation liquid in strain holding vessel is under the effect of gravity with pipeline four The input port one of tee tube is flowed into, the air in desinfection chamber has already passed through sterilization processing, under the action of vacuum pump, air flow direction The input port two of tee tube, fermentation liquid flow out into pipeline one from the delivery outlet of tee tube under the influence of gas, secondly, Cooling body, which carries out cooling treatment to pipeline one, makes fermentation liquid cool down, and prevents temperature is excessively high during air delivery from leading to bacterium Kind is dead, and then, large granular impurity can filter out after fermentation liquid enters cartridge filter, and fermentation liquid is entering separation by pipeline two Cylinder, after the filtering of the filter membrane one of cylinder, filter membrane two, filter membrane three and filter membrane four, the filtrate of macromolecular will be from row The outflow of slag branch pipe, and imports in scum pipe, is stored in transferring box, and the filtrate in transferring box reaches after certain volume from residue outlet Outflow, reenters and carries out repeated filtration separation in pipeline one, to reach maximum separation rate, finally, passing through the small of filter membrane four Molecule filtrate will flow into fluid reservoir with pipeline three.
The cooling body includes cooling tube, and the cooling tube includes ontology and fixed connection at both ends of the body Portion, ontology are cylindrical shape, and interconnecting piece is in a circular table shape, and interconnecting piece has opening far from ontology one end, and opening inner ring is fixed with waterproof Circle, opening outer ring are mutually fixed by the tube body of endless clamp and pipeline one, and a cooling is formed between cooling tube and pipeline one Chamber, inlet opening and apopore are also provided on the tube body of cooling tube, and the side of cooling tube is equipped with a water tank, water tank side tool There is water inlet, the other side of water tank has water outlet, and the top of water tank has a placement mouth for launching coolant, water tank It is inside additionally provided with temperature sensor, temperature sensor connects a temperature indicator by several conducting wires, and temperature indicator is fixed on storage The water inlet of the outer wall of water tank, water tank is communicated by water pipe one with inlet opening, the water outlet of water tank by a water pipe two with Apopore communicates, and flow valve one and water pump one are additionally provided on water pipe one.Water in water tank is under the action of water pump one from water pipe One flows into cooling tube, and water pipe two is then flowed into from cooling tube, and flow valve a pair of water flow size is controlled, and passes through observation Temperature indicator, artificial timing are commercially available existing product, such as liquid to coolant, coolant is launched in water tank Nitrogen, fluorine chlorine are high.
The endless clamp includes work department one, work department two and bolt assembly, and work department one includes in semicircular ring The ontology one of shape, the both ends of ontology one are respectively and fixedly provided with connection sheet one, and screw hole one is offered in connection sheet one, and work department two includes being in The ontology two of semi-annular shape, the both ends of ontology two are respectively and fixedly provided with connection sheet two, and screw hole two, one He of screw hole are offered in connection sheet two Two diameter of screw hole is identical, and screw hole one and screw hole two are arranged concentrically, and passes through bolt assembly connecting screw hole one and screw hole two, one He of ontology Ontology two is made of stainless steel, and ontology one and two inner ring of ontology are fixed with sheet rubber.It is first turned on bolt assembly, by work department One and work department two separate, and then, are set in the opening at cooling tube both ends, tightening bolt assembly makes cooling tube be fixed on pipeline One outside.
The cleaning hole connects one end of a cleaning pipe one, and the other end of cleaning pipe one connects the output of a water pump two End, the input terminal of water pump two connect one end of a cleaning pipe two, and the other end of cleaning pipe two connects a sterile water tank, cleaning pipe Flow valve two is additionally provided on one.After the completion of filtering mask work, opening flow valve two starts water pump two, to the group in device Part is cleaned, the water of use sterilization treatment mistake, it is ensured that next mask work is completed in an aseptic environment.
The filtering bore dia of the filter membrane one is 0.08 μm, and the filtering bore dia of filter membrane two is 0.06 μm, filtering The filtering bore dia of film three is 0.04 μm, and the filtering bore dia of filter membrane four is 0.02 μm.It is below to 0.02 μm according to technique Small molecule is separated.
The upper end of the cartridge filter is hinged with an end cap, and knob is fixed at the top of end cap, and the filtering network edge is solid A fixed pull rod, pull rod top fixed pull ring.After the completion of one action, impurity can be left on filter screen, open end cap at this time, benefit Filter screen is taken out and cleaned with pull ring.
The tube body of the pipeline four is equipped with flow valve three.Flow valve three has the flow of liquid in control pipeline four big Small effect.
The tube body of the pipeline five is equipped with flow valve four.Flow valve four has the flow of liquid in control pipeline five big Small effect.
The residue outlet is equipped with transfer tube and flow valve five.Flow valve five has the flow of liquid in control residue outlet The effect of size.
Compared with prior art, this separation of fermentative broth device has the advantage that
1, the present invention enables the liquid in pipeline one to obtain cold by setting cooling tube, water pump one, water pipe one and water pipe two But, prevent temperature is excessively high during air delivery to cause strain dead.
2, the present invention makes to separate by transferring box, scum pipe, deslagging branch pipe, transfer tube, residue outlet, flow valve five Fermentation liquid can repeatedly filter separation, to reach maximum separation rate.
3, separation small molecule filter is filtered for multiple times by filter membrane one, filter membrane two, filter membrane three and filter membrane four in the present invention Liquid guarantees the quality of the production of product.
4, the visible impurity in filtrate is obtained can in advance by setting cartridge filter, filter screen, pull rod in the present invention Filter, avoids the quality of impurity effect product, and can clean filter screen after filtration, recycles and reduces filter screen more It changes, reduces production cost.
5, the present invention makes the component in equipment complete to filter by the way that sterile water tank, water pump two and flow valve two is arranged It is cleaned after mask work, the water of use sterilization treatment mistake, it is ensured that next mask work is completed in an aseptic environment.
Detailed description of the invention
Fig. 1 is structural schematic diagram of the invention.
Fig. 2 is the structural schematic diagram of cartridge filter of the invention.
In figure, 1, pedestal;2, cylinder;3, filter membrane one;4, filter membrane two;5, filter membrane three;6, filter membrane four;7, in Turnning box;8, scum pipe;9, deslagging branch pipe;10, cartridge filter;10a, stop collar;10b, end cap;11, filter screen;12, pipeline one; 12a, hole for back flow;12b, cleaning hole;13, pipeline two;14, pipeline three;15, pipeline four;16, pipeline five;17, pipeline six;18, it stores up Flow container;19, tee tube;20, vacuum pump;21, desinfection chamber;22, cooling tube;23, water tank;24, water pipe one;25, water pipe two; 26, water pump one;27, flow valve one;28, endless clamp;29, water pump two;30, sterile water tank;31, flow valve two;32, it draws Bar;33, pull ring;34, flow valve three;35, flow valve four;36, transfer tube;37, flow valve five;38, strain holding vessel;39, go out Slag pipe;40, cleaning pipe one;41, cleaning pipe two.
Specific embodiment
Following is a specific embodiment of the present invention in conjunction with the accompanying drawings, technical scheme of the present invention will be further described, However, the present invention is not limited to these examples.
As shown in Figure 1, a kind of separation of fermentative broth device, including pedestal 1, pedestal 1, which is equipped with one, to go out separation of fermentative broth The cylinder 2 of different size particle, cylinder 2 are laterally fixed by bolts on pedestal 1, and one end of cylinder 2 has inlet, The other end of cylinder 2 has liquid outlet, and filter membrane 1, filter membrane two are successively arranged from inlet to liquid outlet in cylinder 2 4, the filtering bore dia of filter membrane 35 and filter membrane 46, filter membrane 1 is 0.08 μm, and the filtering bore dia of filter membrane 24 is 0.06 μm, the filtering bore dia of filter membrane 35 is 0.04 μm, and the filtering bore dia of filter membrane 46 is 0.02 μm, cylinder 2 Offer several mud holes one on outer wall, the side of cylinder 2 is equipped with one for storing the transferring box 7 of waste residue, transferring box 7 with point From scum pipe 8 and several deslagging branch pipes 9 corresponding with mud hole one is equipped between cylinder 2, deslagging branch pipe 9 uses rubber hose system At several mud holes one are corresponded with one end of several deslagging branch pipes 9 to be connected, and scum pipe 8 is stainless steel tube, scum pipe 8 One end be closed end, the other end of scum pipe 8 is outlet end, and scum pipe 8 offers several rows on the tube body of closed end The other end one-to-one correspondence of cinder hole two, several mud holes two and several deslagging branch pipes 9 is connected, transferring box 7 have slag input hole with Scum hole, the outlet end of scum pipe 8 are connected with the slag input hole of transferring box 7, and the side of the inlet of cylinder 2 is equipped with one can mistake The cartridge filter 10 of impurity in fermentation liquid is filtered, as shown in Fig. 2, 10 inner wall of cartridge filter is equipped with a stop collar 10a, is set on stop collar 10a There is a filter screen 11, the outer wall top of cartridge filter 10 offers an import, and the upper end of cartridge filter 10 is hinged with an end cap 10b, end It is fixed with knob at the top of lid 10b, a pull rod 32,32 top fixed pull ring 33 of pull rod are fixed in 11 edge of filter screen.Work as one action After the completion, impurity can be left on filter screen 11, opens end cap 10b at this time, taken out filter screen 11 using pull ring 33 and carried out and is clear It washes, the bottom of cartridge filter 10 offers an outlet, and the outlet of cartridge filter 10 is connected with the inlet of cylinder 2 by pipeline 2 13 Logical, the import of cartridge filter 10 is connected with one end of a pipeline 1, and the side of the liquid outlet of cylinder 2 is equipped with a fluid reservoir 18, it is connected between fluid reservoir 18 and the liquid outlet of cylinder 2 by pipeline 3 14, the other end of pipeline 1 connects a threeway The input port two-way piping 4 15 of the input port one of pipe 19, tee tube 19 connects a strain holding vessel 38, the pipe of pipeline 4 15 Body is equipped with flow valve 3 34;Flow valve 3 34 has the function of controlling the uninterrupted of liquid in pipeline 4 15, tee tube 19 Delivery outlet the output end of a vacuum pump 20 is connected by pipeline 5 16, the tube body of pipeline 5 16 is equipped with flow valve 4 35;Stream Amount valve 4 35 has the function of that the uninterrupted of liquid in control pipeline 5 16, the input terminal of vacuum pump 20 pass through pipeline 6 17 and connect A desinfection chamber 21 is connect, a hole for back flow 12a is offered on the tube body of pipeline 1, hole for back flow 12a is close to 19 one end of tee tube, pipeline A cleaning hole 12b is also provided on one 12 tube body, cleaning hole 12b is between the other end and hole for back flow 12a of pipeline 1, It is communicated between the scum hole of transferring box 7 and hole for back flow 12a by a residue outlet 39, residue outlet 39 is equipped with transfer tube 36 and flow Valve 5 37;Flow valve 5 37 has the function of controlling the uninterrupted of liquid in residue outlet 39, hole for back flow 12a and pipeline 1 The cooling body of an energy cooling and fermentation liquid is additionally provided on tube body between one end, cooling body includes cooling tube 22, cooling tube 22 Including ontology and fixed interconnecting piece at both ends of the body, ontology is cylindrical shape, and interconnecting piece is in a circular table shape, and interconnecting piece is far from ontology one End has opening, and opening inner ring is fixed with water proof ring, and opening outer ring is mutually solid by endless clamp 28 and the tube body of pipeline 1 It is fixed, a cooling chamber is formed between cooling tube 22 and pipeline 1, is also provided with inlet opening and water outlet on the tube body of cooling tube 22 Hole, the side of cooling tube 22 are equipped with a water tank 23, and 23 side of water tank has water inlet, and the other side of water tank 23 has The mouth of a river, the top of water tank 23 have a placement mouth for launching coolant, temperature sensor are additionally provided in water tank 23, and temperature passes Sensor connects a temperature indicator by several conducting wires, and temperature indicator is fixed on the outer wall of water tank 23, water tank 23 into The mouth of a river is communicated by water pipe 1 with inlet opening, and the water outlet of water tank 23 is communicated by a water pipe 2 25 with apopore, water pipe Flow valve 1 and water pump 1 are additionally provided on one 24.Water in water tank 23 flows under the action of water pump 1 from water pipe 1 Enter in cooling tube 22, water pipe 2 25 is then flowed into from cooling tube 22, one 27 pairs of water flow sizes of flow valve control, pass through Temperature indicator is observed, artificial timing is commercially available existing product to coolant, coolant is launched in water tank 23, As liquid nitrogen, fluorine chlorine are high.Pipeline 1, pipeline 2 13, pipeline 3 14, pipeline 4 15, pipeline 5 16, pipeline 6 17 are stainless steel Material is made.
Endless clamp 28 includes work department one, work department two and bolt assembly, and work department one includes in semi-annular shape Ontology one, the both ends of ontology one are respectively and fixedly provided with connection sheet one, and screw hole one is offered in connection sheet one, and work department two includes in semicircle Cricoid ontology two, the both ends of ontology two are respectively and fixedly provided with connection sheet two, and screw hole two, screw hole one and screw hole are offered in connection sheet two Two diameters are identical, and screw hole one and screw hole two are arranged concentrically, by bolt assembly connecting screw hole one and screw hole two, ontology one and ontology Two are made of stainless steel, and ontology one and two inner ring of ontology are fixed with sheet rubber.It is first turned on bolt assembly, by one He of work department Work department two separates, and then, is set in the opening at 22 both ends of cooling tube, tightening bolt assembly makes cooling tube 22 be fixed on pipeline One 12 outside.
One end of one cleaning pipe 1 of hole 12b connection is cleaned, the other end of cleaning pipe 1 connects the defeated of a water pump 2 29 Outlet, the input terminal of water pump 2 29 connect one end of a cleaning pipe 2 41, and the other end of cleaning pipe 2 41 connects a sterile water storage Case 30 is additionally provided with flow valve 2 31 in cleaning pipe 1.After the completion of filtering mask work, opening flow valve 2 31 starts water 2 29 are pumped, the component in device is cleaned, the water of use sterilization treatment mistake, it is ensured that next mask work is sterile It is completed under environment.
This separation of fermentative broth device, its working principle is that such: firstly, strain is stored in strain storage after the fermentation has been completed Deposit in tank 38, strain holding vessel 38 is placed on relatively high position, the fermentation liquid in strain holding vessel 38 under the effect of gravity with Pipeline 4 15 flows into the input port one of tee tube 19, and the air in desinfection chamber 21 has already passed through sterilization processing, in vacuum pump 20 Under effect, air flows to the input port two of tee tube 19, and fermentation liquid is flowed out from the delivery outlet of tee tube 19 under the influence of gas And enter in pipeline 1, so that fermentation liquid is cooled down secondly, cooling tube 22 carries out cooling treatment to pipeline 1, prevents defeated in air Temperature is excessively high during sending causes strain dead, and then, large granular impurity can filter out after fermentation liquid enters cartridge filter 10, hair Zymotic fluid is entering cylinder 2 by pipeline 2 13, in the filter membrane 1 of cylinder 2, filter membrane 24, filter membrane 35 and filtering After the filtering of film 46, the filtrate of macromolecular will be flowed out from deslagging branch pipe 9, and import in scum pipe 8, be stored in transferring box 7, Filtrate in transferring box 7 is flowed out after reaching certain volume from residue outlet 39, reenters in pipeline 1 and carries out repeated filtration point From to reach maximum separation rate, finally, passing through the small molecule filtrate of filter membrane 46 will flow into fluid reservoir 18 with pipeline 3 14.
Specific embodiment described herein is only an example for the spirit of the invention.The neck of technology belonging to the present invention The technical staff in domain can make various modifications or additions to the described embodiments or replace by a similar method In generation, however, it does not deviate from the spirit of the invention or beyond the scope of the appended claims.
Although pedestal 1 is used more herein;Cylinder 2;Filter membrane 1;Filter membrane 24;Filter membrane 35;Filtering Film 46;Transferring box 7;Scum pipe 8;Deslagging branch pipe 9;Cartridge filter 10;Stop collar 10a;End cap 10b;Filter screen 11;Pipeline 1; Hole for back flow 12a;Clean hole 12b;Pipeline 2 13;Pipeline 3 14;Pipeline 4 15;Pipeline 5 16;Pipeline 6 17;Fluid reservoir 18;Three Siphunculus 19;Vacuum pump 20;Desinfection chamber 21;Cooling tube 22;Water tank 23;Water pipe 1;Water pipe 2 25;Water pump 1;Flow valve One 27;Endless clamp 28;Water pump 2 29;Sterile water tank 30;Flow valve 2 31;Pull rod 32;Pull ring 33;Flow valve 3 34; Flow valve 4 35;Transfer tube 36;Flow valve 5 37;Strain holding vessel 38;Residue outlet 39;Cleaning pipe 1;Cleaning pipe 2 41 etc. Term, but it does not exclude the possibility of using other terms.The use of these items is only for be more convenient to describe and explain Essence of the invention;Being construed as any additional limitation is disagreed with spirit of that invention.

Claims (7)

1. a kind of separation of fermentative broth device, including pedestal, which is characterized in that the pedestal is equipped with one can be by separation of fermentative broth The cylinder of different size particle out, the cylinder are secured transverse on pedestal, and one end of cylinder has inlet, point The other end from cylinder has liquid outlet, and filter membrane one, filter membrane are successively arranged from inlet to liquid outlet in the cylinder Two, filter membrane three and filter membrane four, several mud holes one are offered on the outer wall of cylinder, and the side of cylinder is equipped with one and is used for The transferring box for storing waste residue is equipped with scum pipe and several deslagging branch corresponding with mud hole one between transferring box and cylinder Pipe, several mud holes one are corresponded with one end of several deslagging branch pipes to be connected, and one end of scum pipe is closed end, scum pipe The other end be outlet end, scum pipe offers several mud holes two on the tube body of closed end, if several mud holes two with The other end one-to-one correspondence of dry deslagging branch pipe is connected, and transferring box has slag input hole and scum hole, and the outlet end of scum pipe is in The slag input hole of turnning box is connected, and the side of the inlet of cylinder is equipped with the cartridge filter of impurity in an energy filtering fermentating liquid, filtering Cylinder inner wall is equipped with a stop collar, and stop collar is equipped with a filter screen, and the outer wall top of cartridge filter offers an import, cartridge filter Bottom offers an outlet, and the outlet of cartridge filter is connected with the inlet of cylinder by pipeline two, the import of cartridge filter with One end of one pipeline one is connected, and the side of the liquid outlet of cylinder is equipped with a fluid reservoir, the liquid outlet of fluid reservoir and cylinder Between be connected by pipeline three, the other end of pipeline one connects the input port one of a tee tube, the input port two-way of tee tube Piping four connects a strain holding vessel, and the delivery outlet of tee tube connects the output end of a vacuum pump, vacuum pump by pipeline five Input terminal one desinfection chamber is connected by pipeline six, offer a hole for back flow on the tube body of pipeline one, the hole for back flow is close Tee tube one end, is also provided with a cleaning hole on the tube body of pipeline one, and the cleaning hole is in the other end of pipeline one and returns It between fluid apertures, is communicated between the scum hole and hole for back flow of transferring box by a residue outlet, the one of the hole for back flow and pipeline one The cooling body of an energy cooling and fermentation liquid is additionally provided on tube body between end, the cooling body includes cooling tube, described Cooling tube includes ontology and fixed interconnecting piece at both ends of the body, and ontology is cylindrical shape, and interconnecting piece is in a circular table shape, and interconnecting piece is separate Ontology one end has opening, and opening inner ring is fixed with water proof ring, and opening outer ring passes through the tube body phase of endless clamp and pipeline one It is fixed, a cooling chamber is formed between cooling tube and pipeline one, is also provided with inlet opening and apopore on the tube body of cooling tube, it is cold But the side of pipe is equipped with a water tank, and water tank side has water inlet, and the other side of water tank has water outlet, water tank Top has a placement mouth for launching coolant, and temperature sensor is additionally provided in water tank, and temperature sensor passes through several conducting wires A temperature indicator is connected, temperature indicator is fixed on the outer wall of water tank, and the water inlet of water tank passes through water pipe one and water inlet Hole communicates, and the water outlet of water tank is communicated by a water pipe two with apopore, and flow valve one and water pump one are additionally provided on water pipe one, The endless clamp includes work department one, work department two and bolt assembly, and work department one includes the ontology in semi-annular shape One, the both ends of ontology one are respectively and fixedly provided with connection sheet one, and screw hole one is offered in connection sheet one, and work department two includes in semi-annular shape Ontology two, the both ends of ontology two are respectively and fixedly provided with connection sheet two, and screw hole two is offered in connection sheet two, and screw hole one and screw hole two are straight Diameter is identical, and screw hole one and screw hole two are arranged concentrically, and by bolt assembly connecting screw hole one and screw hole two, ontology one and ontology two are adopted It is made of stainless steel, ontology one and two inner ring of ontology are fixed with sheet rubber.
2. separation of fermentative broth device according to claim 1, which is characterized in that the cleaning hole connects a cleaning pipe one One end, the other end of cleaning pipe one connects the output end of a water pump two, and the input terminal of water pump two connects the one of a cleaning pipe two End, the other end of cleaning pipe two connect a sterile water tank, are additionally provided with flow valve two in cleaning pipe one.
3. separation of fermentative broth device according to claim 1, which is characterized in that the filtering bore dia of the filter membrane one It is 0.08 μm, the filtering bore dia of filter membrane two is 0.06 μm, and the filtering bore dia of filter membrane three is 0.04 μm, filter membrane four Filtering bore dia is 0.02 μm.
4. separation of fermentative broth device according to claim 1, which is characterized in that the upper end of the cartridge filter is hinged with one End cap, end cap top are fixed with knob, and the filtering network edge fixes a pull rod, pull rod top fixed pull ring.
5. separation of fermentative broth device according to claim 1, which is characterized in that the tube body of the pipeline four is equipped with stream Measure valve three.
6. separation of fermentative broth device according to claim 5, which is characterized in that the tube body of the pipeline five is equipped with stream Measure valve four.
7. separation of fermentative broth device according to claim 6, which is characterized in that the residue outlet be equipped with transfer tube and Flow valve five.
CN201710098811.3A 2017-02-23 2017-02-23 A kind of separation of fermentative broth device Active CN106834086B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201710098811.3A CN106834086B (en) 2017-02-23 2017-02-23 A kind of separation of fermentative broth device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201710098811.3A CN106834086B (en) 2017-02-23 2017-02-23 A kind of separation of fermentative broth device

Publications (2)

Publication Number Publication Date
CN106834086A CN106834086A (en) 2017-06-13
CN106834086B true CN106834086B (en) 2019-03-08

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CN204455079U (en) * 2015-02-10 2015-07-08 厦门昊源膜科技有限公司 A kind of Cordyceps militaris (L.) Link. separation of fermentative broth is purified and concentrating unit
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