CN106811477A - 一种葡糖苷酶多拷贝表达提高里氏木霉纤维素酶酶活的方法 - Google Patents
一种葡糖苷酶多拷贝表达提高里氏木霉纤维素酶酶活的方法 Download PDFInfo
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- CN106811477A CN106811477A CN201710065834.4A CN201710065834A CN106811477A CN 106811477 A CN106811477 A CN 106811477A CN 201710065834 A CN201710065834 A CN 201710065834A CN 106811477 A CN106811477 A CN 106811477A
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- glucosidase
- trichoderma reesei
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
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Abstract
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Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201710065834.4A CN106811477B (zh) | 2017-02-06 | 2017-02-06 | 一种葡糖苷酶多拷贝表达提高里氏木霉纤维素酶酶活的方法 |
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CN201710065834.4A CN106811477B (zh) | 2017-02-06 | 2017-02-06 | 一种葡糖苷酶多拷贝表达提高里氏木霉纤维素酶酶活的方法 |
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CN106811477A true CN106811477A (zh) | 2017-06-09 |
CN106811477B CN106811477B (zh) | 2019-11-22 |
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CN201710065834.4A Active CN106811477B (zh) | 2017-02-06 | 2017-02-06 | 一种葡糖苷酶多拷贝表达提高里氏木霉纤维素酶酶活的方法 |
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Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1228995A (zh) * | 1999-01-26 | 1999-09-22 | 卢杲 | 一种治疗老年性痴呆症、帕金森症、心脑血管病的药物 |
EP1230343B1 (en) * | 1999-11-19 | 2006-08-30 | Yissum Research Development Company Of The Hebrew University Of Jerusalem | Aspegillus niger beta-glucosidase gene, protein and uses thereof |
CN102409003A (zh) * | 2010-09-26 | 2012-04-11 | 中国农业科学院饲料研究所 | 多拷贝高效表达重组菌丝霉素的毕赤酵母 |
EP3085788A1 (en) * | 2015-04-23 | 2016-10-26 | Institut National De La Recherche Agronomique | Mutant yeast strain capable of degrading cellobiose |
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2017
- 2017-02-06 CN CN201710065834.4A patent/CN106811477B/zh active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1228995A (zh) * | 1999-01-26 | 1999-09-22 | 卢杲 | 一种治疗老年性痴呆症、帕金森症、心脑血管病的药物 |
EP1230343B1 (en) * | 1999-11-19 | 2006-08-30 | Yissum Research Development Company Of The Hebrew University Of Jerusalem | Aspegillus niger beta-glucosidase gene, protein and uses thereof |
CN102409003A (zh) * | 2010-09-26 | 2012-04-11 | 中国农业科学院饲料研究所 | 多拷贝高效表达重组菌丝霉素的毕赤酵母 |
EP3085788A1 (en) * | 2015-04-23 | 2016-10-26 | Institut National De La Recherche Agronomique | Mutant yeast strain capable of degrading cellobiose |
Non-Patent Citations (4)
Title |
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FANJU MENG 等人: "Heterologous protein expression in Trichoderma reesei using the cbhII promoter", 《PLASMID》 * |
李琳 等主编: "《实用医学遗传学》", 31 July 2007, 军事医学科学出版社 * |
林元藻 等主编: "《生化制药学》", 30 June 1998, 人民卫生出版社 * |
陈作国: "响应面法和变温培养优化海洋黑曲霉产纤维素酶发酵条件", 《化学反应工程与工艺》 * |
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CN106811477B (zh) | 2019-11-22 |
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Effective date of registration: 20201118 Address after: 435414 Railway Station Industrial Park, Wushi Avenue, Shifosi Town, Wuxue City, Huanggang City, Hubei Province Patentee after: HUBEI ZHONGNONG HUAWEI BIOLOGICAL ENGINEERING Co.,Ltd. Address before: 430068 Wuhan Province, Wuchang District, South Lake, Lee Ka pier village, No. 1, No. 1, No. Patentee before: HUBEI University OF TECHNOLOGY |
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Denomination of invention: A method for multi copy expression of glucosidase to improve cellulase activity of Trichoderma reesei Effective date of registration: 20211216 Granted publication date: 20191122 Pledgee: Hubei Wuxue Rural Commercial Bank Co.,Ltd. Pledgor: HUBEI ZHONGNONG HUAWEI BIOLOGICAL ENGINEERING Co.,Ltd. Registration number: Y2021420000145 |