CN106806396A - Fermented ginseng containing the active ginseng saponin(e for enhancing body absorption rate or fermented black red ginseng extract manufacture method - Google Patents
Fermented ginseng containing the active ginseng saponin(e for enhancing body absorption rate or fermented black red ginseng extract manufacture method Download PDFInfo
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Abstract
The present invention provides a kind of fermented ginseng containing the active ginseng saponin(e for enhancing body absorption rate or fermented black red ginseng manufacture method and black red ginseng processing product.The fermented ginseng or fermented black red ginseng manufacture method include：1st step, by ginseng or dark red ginseng crushing process, preparing the ginseng or dark red ginseng of nanometer powder；Second step, after carrying out sterilization treatment to being added water in the ginseng or dark red ginseng of the nanometer powder, the inoculating lactic acid bacterium in the ginseng or dark red ginseng of nanometer powder；Third step, cultivates lactic acid bacteria, the ginseng of nanometer powder or dark red ginseng is fermented；4th step, the nutrient solution to being obtained after third step carries out maturation process；And the 5th step, the curing liquid to being obtained after the 4th step carries out sterilization treatment.Therefore, the present invention can provide the fermented ginseng and fermented black red ginseng manufacture method of the active ginseng saponin(e (Rg2, Rh1, Rg3 and 20- ginseng sapoglycoside Rg 3) containing body absorption rate is enhanced.And then, the nutrient solution ripening at low temperature for making to ferment ginseng or red ginseng using lactic acid bacteria such that it is able to improve content that is antifatigue, suppressing metastasis of cancer and the outstanding ginseng sapoglycoside Rg 3 of anticancer effect and 20- ginseng sapoglycoside Rg 3s.In addition, not by neutralization step and Hydrolysis kinetics, with fairly simple operation, manufacture biology is converted to the fermented ginseng and fermented black red ginseng of active ginseng saponin(e, so as to food, cosmetics or pharmaceuticals can also be effectively served as.
Description
Technical field
The present invention relates to fermented ginseng or fermented black red ginseng extract, more specifically, it is related to one kind to contain
Enhance fermented ginseng or the fermented black red ginseng extract manufacturer of the active ginseng saponin(e of body absorption rate
Method.
Background technology
Ginseng is the perennial root grass for belonging to Araliaceae Panax in Plant Taxonomy, on earth
Known about 11 kinds, representative kind has Korean ginseng (Panax ginseng C.A.Meyer), the U.S.
Ginseng (Panaxquinquefolium L.), pseudo-ginseng ginseng (Panaxnotoginseng F.H.Chen),
Panax japonicus (Panaxjaponicus C.A.Meyer).Korean ginseng (Panax ginseng C.A.
Meyer Far East Asia (north latitude 33~48, South Korea, Northeast China the north, Russia) are grown on naturally
Ross one), drug effect is very outstanding.U.S.'s ginseng (Panaxquinquefolium L.) the U.S.,
Canada grows and cultivates naturally, and pseudo-ginseng joins (Panaxnotoginseng F.H.Chen) in China
The Yunnan Province southeast to Guangxi province Localities In Southwest is wild or cultivates.In addition, panax japonicus
(Panaxjaponicus C.A.Meyer) is distributed in Japan, southwest China and Nepal.
With the Main Ingredients and Appearance of ginseng and delivering for pharmacological action correlative study, as natural health food
And attract tremendous attention, its demand also gradually increases, further because of economic growth and growth in the living standard
Accelerate, be widely used as popular type natural health food.In addition, ginseng is main in Asias such as South Korea
Continent country is various for psychologic medicine, the nervous system disease and diabetes etc. with the form of crude drug
Disease is used, have report claim, as the ginseng Main Ingredients and Appearance saponin(e to strong, calm, hematopoiesis,
Anti-hypertension and antitumor activity etc. show effect.
The composition and its pharmacology efficiency of the ginsenoside contained in ginseng are as shown in table 1 below.
【Table 1】
In general, fresh ginseng of the ginseng to cultivate and after gathering, making fresh ginseng dry white ginseng at normal temperatures
Or carry out with vapor at about 98 DEG C to 100 DEG C steaming to consider and handle reason after fresh ginseng is cleaned with Purified Water and
The form of the red ginseng of manufacture is used.Wherein, particularly red ginseng is considered as pharmacology efficiency and is far superior to
White ginseng.Recently as causing the efficacy component of this ginseng easily to absorb into the human body, strengthen in people
The method of the composition existed with denier in ginseng, be currently in use is made one using enteric microorganism or lactic acid bacteria
Ginseng fermentation or the method for carrying out ferment treatment.
But, manufacture ginseng sapoglycoside Rg 3 and 20- ginseng sapoglycoside Rg 3 contents ginseng high were extracted in the past
During thing, it is impossible to avoid the such as HMF (hydroxylmethylfurfual) generated in heat treatment process
Carcinogen generation, according to heat and/or acid treatment operation, must by neutralization step, need
Hydrolysis kinetics process is separately performed, thus there is a problem of being not easy being used as direct food.
In addition, the most microorganism used to carry out biological conversion to ginsenoside, as
The Pediococcus (Pediococcussp.) that can not eat, Leuconostoc (Leuconostocsp.),
Eurotium (Aspergillus sp.), Eubacterium (Eubacterium sp.), Fusobacterium
(Fusobacterium sp.) or Bacteroides (Bacteroides sp.) bacterial strain etc., it is necessary to be directed to by
The metabolite of this generation, separately performs ultrafiltration or separation and purification (Hydrolysis kinetics) process, thus is difficult to
Used as direct food, there is a problem of being difficult to being utilized in real life.
【Prior art literature】
【Patent document】
Republic of Korea's registered patent the 10-228510th
Republic of Korea's registered patent the 10-479803rd
Republic of Korea's registered patent the 10-856790th
Republic of Korea's registered patent the 10-866504th
【Non-patent literature】
1. Hasegawa H.,J.Pharmacol.Sci.,2004,95(2):153-157.
2. Tawab M.A.et al.,Drug Metab.Dispos.,2003,
31(8):1065-1071.
The content of the invention
The invention technical problem to be solved
The problem to be solved by the present invention is that, there is provided one kind utilizes lactic acid bacteria, ginseng, red ginseng or dark red
The saponin(e contained in ginseng, i.e. intrinsic ginsenoside is converted into as the activity to the more useful form of human body
Fermented ginseng and fermented black the red ginseng manufacture of ginsenoside (Rg2, Rh1, Rg3 and 20- ginseng sapoglycoside Rg 3)
Method.
The invention solves the problems that another problem be, not by neutralization step and Hydrolysis kinetics, but with than
Better simply operation, manufacture biology is converted into the fermented ginseng and fermented black red ginseng of active ginseng saponin(e.
The technical scheme of solve problem
To realize the problem, one aspect of the present invention provides a kind of fermented ginseng or fermented black red ginseng is carried
Take thing manufacture method.The fermented ginseng or fermented black red ginseng extract manufacture method can include：1st
Step, by ginseng or dark red ginseng crushing process, preparing the ginseng or dark red ginseng of nanometer powder；The
2 steps, after carrying out sterilization treatment to being added water in the ginseng or dark red ginseng of the nanometer powder, described
Inoculating lactic acid bacterium in the ginseng of nanometer powder or dark red ginseng；Third step, cultivates the lactic acid bacteria, makes
The ginseng of the nanometer powder or dark red ginseng are fermented；4th step, to what is obtained after the third step
Nutrient solution carries out maturation process；And the 5th step, the curing liquid to being obtained after the 4th step goes out
Bacterium is processed.
1st step can include：The ginseng or dark red ginseng are ground into 2mm to 5mm for the first time
The step of；And the ginseng or dark red ginseng carried out after the step of crushing for the first time is ground into 0.3 for the second time
The step of μm to 15 μm.
Nutrient solution to being obtained after the third step carries out the 4th step of maturation process, can be at 35 DEG C
Carried out to 45 DEG C 12 hours to 72 hours.
The fermented ginseng or fermented black red ginseng can be containing selected from by compound K (20-O- β-D- pyrans Portugals
Grape glycosyl -20 (S)-protopanoxadiol), ginsenoside Rh 1, ginseng saponin Rh 2, ginsenoside Rg2 and
At least one fermentation composition of the group that 20- ginseng sapoglycoside Rg 3s are constituted.
The lactic acid bacteria can be from by enterococcus (Enterococcus) category, streptococcus (Streptococcus)
Select at least one in the group that category and Bifidobacterium (Bifidobacterium) category are constituted.
Curing liquid to being obtained after the 4th step carries out the 5th step of sterilization treatment, can be at 80 DEG C
Carried out to 95 DEG C 12 hours to 48 hours.
The lactic acid bacteria can be inoculated with 1% to 5% concentration.
The lactic acid bacteria can cultivate 1 day to 5 day time.
5th step is centrifuged after may additionally include the sterilization treatment, to the upper strata for obtaining
After liquid is cured, concentrated or freeze-dried step.
The effect of invention
According to the present invention it is possible to provide it is a kind of containing enhance body absorption rate active ginseng saponin(e (Rg2,
Rh1, Rg3 and 20- ginseng sapoglycoside Rg 3) fermented ginseng and fermented black red ginseng manufacture method.And then, profit
Make the nutrient solution ripening at low temperature fermented to ginseng or red ginseng with lactic acid bacteria such that it is able to improve anti-tired
Labor, the content for suppressing metastasis of cancer and the outstanding ginseng sapoglycoside Rg 3 of anticancer effect and 20- ginseng sapoglycoside Rg 3s.
In addition, according to the present invention, not by neutralization step and Hydrolysis kinetics, but with fairly simple work
Sequence, manufacture biology is converted to the fermented ginseng and fermented black red ginseng of active ginseng saponin(e, so as to can also have
Effect ground is used as food, cosmetics or pharmaceuticals.
Technique effect of the invention is not limited to the content that the above is mentioned, other technique effects not mentioned
It is that those skilled in the art can be expressly understood that from following record.
Brief description of the drawings
Fig. 1 is the fermented ginseng and fermented black red ginseng extract manufacture method for showing one embodiment of the invention
Flow chart.
Fig. 2 (Fig. 2 a-2b) is the people of the fermented ginseng of analysis one embodiment of the invention and comparative example respectively
HPLC (High performanceliquid chromatography) chart of the saponin(e of ginseng.
Fig. 3 (Fig. 3 a-3d) be show orally administration one embodiment of the invention fermented ginseng extract and
Active ginseng saponin(e Rg2, Rh1, Rg3 or 20- ginseng occurred respectively during the ginseng extract of comparative example
The chart of saponin concentrations rheological parameters' change with time passage in the blood of saponin(e Rg3.
Specific embodiment
Below with reference to the accompanying drawings, embodiments of the invention are described in detail.
The present invention allows various modifications and deformation, and its specific embodiment has carried out citing diagram by accompanying drawing,
It is specifically described below.But do not really want the present invention to be defined in the meaning of disclosed especially form, conversely,
The present invention includes all modifications consistent with the inventive concept as defined in claim, equalization and replaces
Generation.
These embodiments are served only for further illustrating the present invention, and main idea of the invention is of the invention
Scope is not limited to these embodiments, and this is self-evident person of ordinary skill in the field.
Fig. 1 is the fermented ginseng and fermented black red ginseng extract manufacture method for showing one embodiment of the invention
Flow chart.
If reference picture 1, fermented ginseng of the invention and fermented black red ginseng extract manufacture method include：
1st step (S100), by ginseng or dark red ginseng crushing process, prepare nanometer powder ginseng or
Dark red ginseng；Second step (S200), is gone out to being added water in the ginseng or dark red ginseng of the nanometer powder
After bacterium treatment, the inoculating lactic acid bacterium in the ginseng or dark red ginseng of the nanometer powder；Third step
(S300) lactic acid bacteria, is cultivated, the ginseng of the nanometer powder or dark red ginseng is fermented；4th step
Suddenly (S400), maturation process is carried out to the nutrient solution obtained after the third step；And the 5th step (S500),
Curing liquid to being obtained after the 4th step carries out sterilization treatment.
If more specifically examined or check, first, for the ginseng, ginseng is in itself and processing of Panax ginseng thing is equal
Can be used, be not particularly limited.For example, fresh ginseng, red ginseng, red ginseng tails, black ginseng, black can be included
Ginseng palpus, dark red ginseng, white ginseng, palpus ginseng, ginseng fruit, folium panacis japonici cum caule, ginseng extract or Ginseng Root Powder.
The original producton location of the ginseng is also not particularly limited.For example, Korean ginseng, Radix Notoginseng, American can be included
Ginseng, panax japonicus, Himalaya ginseng or Vietnam's ginseng.In addition, the ginseng can as needed and with
Usual way sterilizing is used.
The dark red ginseng can be raw material ginseng with the ginseng, be formed by dark red ginsengization process.It is first
First, can dry the raw material ginseng so that water content reaches 10% to 14% in ginseng, is made dry
Ginseng form.It is then possible to the trepang is put into closed container together with water, kept under both constant-pressures
Determined temperature is simultaneously heated, and forms dark red ginseng.For example, the pressure of the closed container can be 2.0 X
105The X of Pa to 1.0 106Pa.The heating can keep 85 DEG C to 180 DEG C, and perform 8 hours extremely
12 hours.
Then, the ginseng or dark red ginseng can be crushed at least 2 times.For example, can be the ginseng
Or it is 2mm to 5mm that dark red ginseng is crushed for the first time.It is then possible to the first time crush ginseng or
It is 0.3 μm to 15 μm that dark red ginseng is crushed for second, manufactures the ginseng or dark red ginseng of ultramicro powder.
Now, crushing for described second can use ACM pulverizers, but be not limited to this.
Fermented when using by the ultra tiny ginseng of this nanometer powder process or the execution of black Red Ginseng
Cheng Shi, performance makes to be converted into active ginseng soap as the glucosides ginsenoside of ginseng or dark red ginseng effective ingredient
The biological conversion elevated effect of yield of glycosides.In addition, conventional technology is in order to improve the life of active ginseng saponin(e
Thing changes yield, is fermented using the extract or concentrate of ginseng, conversely, the present invention is without extracting
Process just can manufacture active ginseng saponin(e, before fermentation procedure, be carried out without to ginseng or dark red ginseng
The process extracted and/or concentrate, thus have the advantages that beneficial in terms of manufacturing cost and environmental protection.
In addition, the present invention keeps the ginseng of whole or dark red ginseng, thus except the ginseng soap as effective ingredient
Outside glycosides, can exist as the effective ingredient of ginseng containing such as several mineral materials or acidic polysaccharose naturally
All compositions known to academia, it is thus possible to which the fermented ginseng or fermented black red ginseng for providing higher quality are carried
Take thing.
It is then possible to added water in the ginseng or dark red ginseng of the nanometer powder, after carrying out sterilization treatment,
The inoculating lactic acid bacterium (S200) in the ginseng or dark red ginseng of the nanometer powder.
The lactic acid bacteria can use the lactic acid bacteria with the biological conversion energy of saponin(e.It is just described with saponin(e life
For the lactic acid bacteria of thing conversion energy, as long as the usually used microorganism in fermented ginseng field, then can make
With.
As the lactic acid bacteria with the biological conversion energy of saponin(e, lactobacillus can be included
(Lactobacillus) category, streptococcus (Streptococcus) category or enterococcus (Enterococcus) category.
For example, in the lactobacillus (Lactobacillus) category lactic acid bacteria, Lactobacillus rhamnosus can be included
7469 plants of ATCC (Lactobacillus rhamnose ATCC 7469), Lactobacillus gasseri DSM 20243
Strain (Lactobacillus gasseri DSM 20243), Lactobacillus mali (Lactobacillus gmali
ATCC 27304), (the Lactobacillus plantarum ATCC of Lactobacillus plantarum ATCC 14947
14947), Lactobacillus plantarum ATCC 10241 (Lactobacillus plantarum ATCC 10241)
Or lactobacillus lactis (Lactobacillus lactis).In addition, at streptococcus (Streptococcus)
In category lactic acid bacteria, streptococcus thermophilus (Streptococcus thermophilus) can be included.
In addition, the lactic acid bacteria with the biological conversion energy of saponin(e both can be used alone, it is also possible to as
Hybrid bacterial strain is used, it is preferable that can include VREF (Enterococcus faecium), excrement intestines
Coccus (Enterococcus faecalis), Lactobacillus casei (Lactobacillus casei), thermophilic breast
Acidfast bacilli (Lactobacillus acidophillus), bifidobacterium longum (Bifidobacterium
) and bifidobacterium infantis (Bifidobacterium infantis) longum.
As the lactic acid used in fermented ginseng of the invention and fermented black red ginseng extract manufacture method
Bacterium, it is preferable that can be from by enterococcus (Enterococcus) category, streptococcus (Streptococcus)
One or more the breast selected in the group that category and Bifidobacterium (Bifidobacterium) category are constituted
Sour bacterium.
For example, as the lactic acid bacteria, it is possible to use VREF (Enterococcus faecium),
Enterococcus faecalis (Enterococcus faecalis).
The lactic acid bacteria can be inoculated with 1% to 5% concentration.
It is then possible to cultivate the lactic acid bacteria, the ginseng of the nanometer powder or dark red ginseng is set to ferment
(S300).More specifically, the lactic acid bacteria of inoculation can be cultivated 1 day to 5 day time.Thus,
The ginseng of the nanometer powder or dark red ginseng can be made to ferment.Furthermore it is possible to make the ginseng of fermentation or black
The content of the ginsenoside of red ginseng increases.
It is then possible to the nutrient solution that the ginseng or dark red ginseng to the nanometer powder are obtained after fermenting is carried out
Maturation process (S400).At a certain temperature, the ginseng of fermentation or dark red ginseng nutrient solution curing both timings are made
Between such that it is able to the fermenting enzyme existed using lactic acid bacteria inside.The fermented ginseng ferments dark red
Ginseng can contain by compound K ((the S)-protopanoxadiol of 20-O- β-D- glucopyranosyls -20), ginseng
The group that saponin(e Rh1, ginseng saponin Rh 2, ginsenoside Rg2 and 20- ginseng sapoglycoside Rg 3s are constituted is at least
More than one fermentation composition.Can particularly improve be considered as it is antifatigue compared with other ginsenosides,
Suppress metastasis of cancer and more excellent Rg2, Rg3, Rh1 and 20-Rg3 content of anti-glycosuria effect.
For example, the maturation process can be carried out 12 hours to 72 hours at 35 DEG C to 45 DEG C.
It is then possible to the curing liquid to being obtained after the maturation process carries out sterilization treatment (S500).It is described
Sterilization treatment can off and on be carried out 12 hours to 48 hours at 80 DEG C to 95 DEG C.Now, may be used
It is centrifuged with to the nutrient solution obtained after sterilization treatment, the upper liquid to obtaining is concentrated or frozen
Knot drying, manufactures fermented black red ginseng extract.
The fermented ginseng and fermented black red ginseng extract manufacture method of one embodiment of the invention, it is possible to use
Fairly simple operation including ripening at low temperature treatment is manufactured using the ginseng as ginseng or red ginseng effective ingredient
Saponin(e biology is converted into enhancing the fermented ginseng and fermented red ginseng of the active ginseng saponin(e of body absorption rate.
Therefore, it can be generated such as in heat treatment process in the conventional fermented ginseng manufacturing process of exclusion
The generation of the carcinogen of HMF (Hydroxylmethylfurfual), additionally it is possible to exclude according to heat and/or acid
Treatment process and the neutralization step that has to pass through, can provide need not move through Hydrolysis kinetics just can be easily
The fermented ginseng and fermented black red ginseng extract manufacture method utilized in real life.
In addition, lactobacillus of the culture as the lactic acid bacteria for specifically being bred in dark red ginseng
(Latobacillussp) and Bifidobacterium (Bifidobaterium sp.) microorganism, ginsenoside is made
The metabolite of Rg1, Rb1 etc. is converted into the compositions such as ginseng sapoglycoside Rg 3, compound K, GF1,
Such that it is able to manufacture containing organism utilization rate ginsenoside composition high and make the antimicrbial power of ginseng extract
The streptococcus acidi lactici fermented solution of raising, fermentation powder, concentrate and fermentation live bacteria agent, and can manufacture containing
Its composition.
And then, due to outside without except black red ginseng extract for cultivate other culture mediums of lactic acid bacteria into
Part, it is thus possible to simplify operation, performance can reduce because of the effect of product content loss caused by additive.
<Embodiment 1>
Fermented black red ginseng extract manufacture method
4 ginsengs more than end of the year are chosen to be raw material ginseng and are cleaned up.
The raw material ginseng is dried, the content of moisture in ginseng is reached less than 14%.
The trepang formed by the drying and water are together put into closed container, 1.0 X are kept
106The high pressure conditions of Pa.Now, water is injected to the degree for not submerging the trepang.Then, keeping
After 10 hours are heated while 125 DEG C of temperature, dark red ginseng is formed.
Then, the dark red ginseng of the formation is put into pulverizer, 46 μm to 74 μm is crushed to for the first time
First time crushed material, is put into ACM pulverizers by the particle of size, and 13 μm to 23 μ are crushed to for the second time
After the particle of m sizes, the dry type or wet ball mill of second crushed material input coating polyurethane, the
Black Red Ginseng is used in the nano particle for being crushed to 500nm sizes three times, manufacture fermented black red ginseng manufacture.
Then, water 250ml is added in the black Red Ginseng 50g of the manufacture, is removed using nitrogen and sent out
After the dissolved oxygen of the nutrient solution of ferment machine, sterilized at 85 DEG C.Then, dung intestines ball is inoculated with about 5% concentration
After bacterium (Enterococcus faecium), stirred at 37 DEG C and cultivated 2 day time.
Then, after being depressurized to incubator, with the air inside microculture nitrogen displacement, so
Afterwards 24 hours are cured in a low temperature of about 25 DEG C.
Nutrient solution to being obtained after the maturation process is concentrated in vacuo at 85 DEG C, has manufactured fermented black
Red ginseng extract (that is, fermented black red ginseng).
<Embodiment 2>
Fermentation American Black red ginseng extract manufacture method
Korean ginseng is substituted using U.S.'s ginseng (American ginseng), the U.S. is manufactured in the same manner as in Example 1
After dark red ginseng, VREF is substituted except enterococcus faecalis (Enterococcus faecalis) is used
Outside (Enterococcus faecium), in the same manner as in Example 1, American Black red ginseng is entered
Row fermentation, has manufactured fermentation American Black red ginseng.
<Embodiment 3>
The fermentation dark red conopsea extraction manufacture method of ring
Korean ginseng is substituted using panax japonicus, after manufacturing the dark red ginseng of ring in the same manner as in Example 1,
VREF (Enterococcus is substituted except lactoenterococcus (Enterococcus lactis) are used
Faecium outside), the dark red ginseng of fermentation ring has been manufactured in the same manner as in Example 1.
<Embodiment 4>
The fermentation dark red conopsea extraction manufacture method of Himalaya
Korean ginseng is substituted using Himalaya ginseng, fermentation happiness has been manufactured in the same manner as in Example 1
The refined dark red ginseng of horse traction.
<Embodiment 5>
The dark red conopsea extraction manufacture method of fermentation Vietnam
In addition to substituting Korean ginseng using Vietnam's ginseng and using the dark red ginseng of Vietnam as in embodiment 1,
Fermented ginseng has been manufactured in the same manner as in Example 1.
Fig. 2 a and Fig. 2 b are that the conduct fermentation of analysis comparative example 1 and embodiments of the invention 1 respectively is dark red
Join HPLC (High performanceliquid chromatography) chart of the saponin(e of effective ingredient.
<Comparative example 1>
By heat treatment and the ginseng extract manufacture method of Solvent Extract methods operation
Fresh ginseng 100g is put into closed container, 2 hours are heated at 130 DEG C.
The ginseng of the processing is extracted with methyl alcohol 200ml, methanolic extract is obtained, makes methyl alcohol evaporative removal
Afterwards, remaining residue is suspended in water 100ml, with extracted by ether 3 times, use 100ml ether every time,
Then remaining water layer is extracted 3 times with water saturation butanol, 100ml water saturation butanol is used every time, obtained
Butanol extract containing saponin(e (with reference to No. 228,510 embodiment 1 of Republic of Korea registered patent).
In the 228th, No. 510 embodiment 1 of Republic of Korea's registered patent, the post for obtaining Rg3 classifications
Type chromatography operation is omitted for direct comparing the with extract.
<Comparative example 2>
Using the ginseng extract manufacture method of lactobacillus-fermented
6 end of the year white ginseng 500g bought from Jingdone district market are chopped up, after extracting 5 times with MeOH 5L, will
It is concentrated under reduced pressure, and obtains 25g.After being dissolved in water 30ml, extracted 4 times with BuOH 1500ml
And concentrate, obtain ginseng BuOH classifications 10g.Bifidobacterium KK-1 (storage numberings are put into wherein：KCCM
10364) with Bifidobacterium KK-2 (storage numberings：KCCM 10365) each 10g (wet type weight), at 37 DEG C
After lower culture 72 hours, extracted with BuOH, concentrated, obtain the ginseng 4g of working process (with reference to big
No. 479,803 embodiment 1 of Han Minguo registered patents)
<Comparative example 3>
Using the fermented ginseng extract manufacture method of lactobacillus lactobacillus-fermented
Ginseng concentrate with 10 times of distilled water diluting after, under 121 DEG C, 1.5 air pressure carrying out high temperature adds
Pressure is processed and 15 minutes that sterilize.Then, it is inoculated with the ginseng concentrate of sterilizing of the invention
Lactobacillus alimentarius M-2 or Leuconostoc mensenteroides M-3 bacterium
Strain.Inoculum concentration relative to ginseng concentrate entirety volume be 1% (v/v) (equivalent to 107-108cfu).Inoculation
Afterwards, 120 hours have been cultivated at 30 DEG C (with reference to Republic of Korea's the 856th, No. 790 reality of registered patent
Apply example 4)
<Comparative example 4>
Using the fermented red ginseng extract manufacture method of lactobacillus lactobacillus-fermented
PBS (50mM, pH 5.0) 50 is added in the Red Ginseng 5g bought from Jin Shan
After mL is suspended, pressurization sterilizing is carried out.In the red ginseng suspension of pressurization sterilization treatment, respectively by total
2% level of nutrient solution, is inoculated with the isolated strains plant of the preculture (preculture) from MRS culture mediums
Lactobacillus P2 and Lactobacillus brevis M2, slowly stirs at 37 DEG C and cultivates 5 day time, has manufactured red ginseng
Fermentate.Inoculated plant lactobacillus P2 and the red ginseng that ferments is designated " P2 fermented red ginsengs ", be inoculated with short breast
Bacillus M2 and the red ginseng that ferments are designated " M2 fermented red ginsengs ".
In addition, after red ginseng extract (60brix) is diluted to 15brix, pH being adjusted to 6.0 and being entered
Row pressurization sterilizing.In the red ginseng suspension of pressurization sterilization treatment, respectively by 2% level of total nutrient solution,
Isolated strains P2, M2 of preculture in MRS are seeded in, are slowly stirred at 37 DEG C and cultivated 5 day time,
Red ginseng fermentate has been manufactured (with reference to No. 866,504 embodiment 3 of Republic of Korea's registered patent).
<Test example 1>
Measure the composition change of ginseng extract
Embodiments of the invention 1,2 and 3 are measured to be carried with the ginseng of acquisition in comparative example 1,2,3 and 4
Take the composition change of thing.
Each ginseng extract 5g that precision weighing is obtained, is put into the concentration flask of 100ml.Then,
After concentrated under reduced pressure, water saturation butanol 50ml is added, adhere to reverse-flow type cooler, in a water bath, 70
DEG C~80 DEG C at heating extraction cooled down after about 1 hour, then filtered, to residue continue enter repeatedly
Gone it is this operation 2 times.Filter paper 10 DEG C of cleanings of water saturation butanol, merge filtrate with washing lotion, add
250ml separatory funnels, are fully vibrated with water 20ml, washed.In advance by the full liquid of water saturation butanol extract
The concentration flask as constant is moved to, is concentrated under reduced pressure in a water bath, after removal butanol, in its residual
Ether 50ml is put into thing, adheres to reverse-flow type cooler, in a water bath, heated 30 minutes at 36 DEG C
And after causing degreasing, eliminate ether.10 times of methyl alcohol is added in the residue for obtaining, completely molten
Xie Hou, is filtered using filter (0.45 μm), is then quantitatively divided with high performance liquid chromatograph (HPLC)
Ginsenoside composition is analysed, its result is as shown in table 2 below.The HPLC conditions are as follows.Machine：Shimadzu
Prominence
Pillar：Gromsil ODS 4.6(i,d)×250mm
Mobile phase A：Acetonitrile:Distilled water=15:85
Mobile phase B：Acetonitrile:Distilled water=80:20
Flow velocity：1.0/min
Detector：ELSD(Alltech)
【Table 2】
As depicted in table 2 it has been confirmed that just without the fermentation by means of lactic acid bacteria or intestinal bacterium,
But for the ginseng extract of the comparative example 1 manufactured by heat treatment and Solvent Extract methods operation,
The content of ginseng sapoglycoside Rg 3 is although of a relatively high, but is not worth the level for meeting, just through peracid treatment
And lactobacillus-fermented and for the ginseng extract of comparative example 2 that manufactures, compared with comparative example 1, ginseng
The content of saponin(e Rg3 increases, but Rg2, Rh1 and 20- ginseng sapoglycoside Rg 3 content are still relatively low.In addition,
Carried by the fermented ginseng of the comparative example 3 and 4 of lactobacillus (Lactobacillus sp.) category lactobacillus-fermented
Thing is taken, compared with comparative example 1 and 2, relatively similar level is shown.
In contrast, according to the present invention and manufacture fermented ginseng and fermented red ginseng extract (embodiment) with
Comparative example is compared, and the content of active ginseng saponin(e Rg2, Rh1, Rg3 and 20-Rg3 shows of a relatively high
Level.
Fig. 3 is the people of the fermented ginseng extract and comparative example for showing orally administration one embodiment of the invention
Active ginseng saponin(e Rg2, Rh1, Rg3 or 20- ginseng sapoglycoside Rg 3 occurred respectively during conopsea extraction
The chart of saponin concentrations rheological parameters' change with time passage in blood.
<Test example 2>
Measure the organism utilization rate of effective ginsenoside
The ginseng extract 40g manufactured in embodiment 1, an orally administration 60 to 70kg it is strong
Health adult (male, n=3) after, 0.25 hour, 0.5 hour, 1 hour, 2 hours, 4 hours, 6
Hour, 8 hours, 12 hours, 24 hours, collection blood sample (5ml) are analyzed present in blood
Saponin(e and saponin(e metabolism body.
Blood 5ml is gathered by different time, analysis condition is using SPE machine (Solid phase
Extraction), after carrying out Hydrolysis kinetics to ginsenoside, methyl alcohol is added, makes to be adsorbed in SPE machine
The ginsenoside dissolution of adsorbent, after being filtered using filter (0.45 μm), uses high performance liquid chromatography
Instrument (HPLC) quantitative analysis ginsenoside composition.The result of the rheological parameters' change with time of saponin derivative in measurement blood
As shown in Figure 3.
Be considered as the saponin(e of ginseng pharmacology composition, be made up of glycosidic structure, it is known that saponin(e its own do not inhaled
Receive, by means of intestinal microbial, biology is converted into saponin(e metabolin (compound K, GF1 etc.)
Afterwards, body absorption rate promoted (Hasegawa H., J.Pharmacol.Sci., 2004,
95(2):153-157；Tawab M.A.et al.,Drug Metab.Dispos.,2003,
31(8):1065-1071.)。
As in figure 3 it has been confirmed that at 30 minutes to 2 hours, active ginseng saponin(e Rg2, Rh1,
Rg3 and 20 ginseng sapoglycoside Rg 3 compositions show absorption maximum respectively, when compare embodiments of the invention with
Understood during comparative example, the body absorption rate of fermented black red ginseng extract of the invention increases more than 20 times.
On the other hand, the embodiments of the invention disclosed in the specification and drawings are only to aid in
Understand and propose particular example, do not really want to limit the scope of the present invention.In addition to the embodiment disclosed herein,
Other variations based on the technology of the present invention thought can also be implemented, and this is the technical field of the invention
Technical staff it is self-evident.
Claims (9)
1. a kind of fermented ginseng or fermented black red ginseng extract manufacture method, it is characterised in that including：
1st step, by ginseng or dark red ginseng crushing process, preparing the ginseng or black of nanometer powder
Red ginseng；
Second step, after carrying out sterilization treatment to being added water in the ginseng or dark red ginseng of the nanometer powder,
The inoculating lactic acid bacterium in the ginseng or dark red ginseng of the nanometer powder；
Third step, cultivates the lactic acid bacteria, the ginseng of the nanometer powder or dark red ginseng is fermented；
4th step, the nutrient solution to being obtained after the third step carries out maturation process；And
5th step, the curing liquid to being obtained after the 4th step carries out sterilization treatment.
2. fermented ginseng according to claim 1 or fermented black red ginseng extract manufacture method, it is special
Levy and be,
1st step includes：
The step of ginseng or dark red ginseng are ground into 2mm to 5mm for the first time；And
The ginseng or dark red ginseng carried out after the step of crushing for the first time is ground into 0.3 μm for the second time
The step of to 15 μm.
3. fermented ginseng according to claim 1 or fermented black red ginseng extract manufacture method, it is special
Levy and be,
Nutrient solution to being obtained after the third step carries out the 4th step of maturation process, at 35 DEG C to 45
Carried out at DEG C 12 hours to 72 hours.
4. fermented ginseng according to claim 1 or fermented black red ginseng extract manufacture method, it is special
Levy and be,
The fermented ginseng or fermented black red ginseng contain selected from by compound K (20-O- β-D- glucopyranoses
Base -20 (S)-protopanoxadiol), ginsenoside Rh 1, ginseng saponin Rh 2, ginsenoside Rg2 and 20-
At least one fermentation composition of the group that ginseng sapoglycoside Rg 3 is constituted.
5. fermented ginseng according to claim 1 or fermented black red ginseng extract manufacture method, it is special
Levy and be,
The lactic acid bacteria from by enterococcus (Enterococcus) category, streptococcus (Streptococcus) category and
Select at least one in the group that Bifidobacterium (Bifidobacterium) category is constituted.
6. fermented ginseng according to claim 1 or fermented black red ginseng extract manufacture method, it is special
Levy and be,
Curing liquid to being obtained after the 4th step carries out the 5th step of sterilization treatment, at 80 DEG C to 95
Carried out at DEG C 12 hours to 48 hours.
7. fermented ginseng according to claim 1 or fermented black red ginseng extract manufacture method, it is special
Levy and be,
The lactic acid bacteria is inoculated with 1% to 5% concentration.
8. fermented ginseng according to claim 1 or fermented black red ginseng extract manufacture method, it is special
Levy and be,
The lactic acid bacteria cultivates 1 day to 5 day time.
9. fermented ginseng according to claim 1 or fermented black red ginseng extract manufacture method, it is special
Levy and be,
5th step is centrifuged after being additionally included in the sterilization treatment, to the upper liquid for obtaining
After being cured, concentrated or freeze-dried step.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107550961A (en) * | 2017-10-24 | 2018-01-09 | 东方红（通化）生物医药股份有限公司 | A kind of method that microbe Rapid Fermentation prepares ginseng/American ginseng extract |
| CN107619847A (en) * | 2017-11-09 | 2018-01-23 | 宁波润沃生物科技有限公司 | A kind of method that biofermentation prepares panaxoside metabolin |
| CN107779475A (en) * | 2017-09-30 | 2018-03-09 | 安徽利民生物科技股份有限公司 | A kind of extracting method of sponge gourd saponin(e |
| CN109043535A (en) * | 2018-07-26 | 2018-12-21 | 哈尔滨工业大学（威海） | A kind of American ginseng extract rich in rare ginsenoside |
| CN110664853A (en) * | 2018-07-03 | 2020-01-10 | 延边可喜安生物科技有限公司 | Method for extracting red ginseng saponin |
| CN112335875A (en) * | 2019-08-09 | 2021-02-09 | 株式会社艾福瑞特 | Health functional composition with blood circulation improving effect |
| CN113631146A (en) * | 2019-06-14 | 2021-11-09 | 自然灵感之园有限公司 | Whole plant extract containing root fermented product of ginseng cultivated by cold wind and cosmetic composition containing the same |
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| KR102015783B1 (en) * | 2019-02-08 | 2019-10-23 | 제너럴네이처주식회사 | Black ginseng fermented jelly and its manufacturing method |
| KR102053735B1 (en) * | 2019-02-11 | 2019-12-09 | 제너럴네이처주식회사 | Black ginseng lactic acid bacteria fermentation composition and method for producing the same |
| KR102429921B1 (en) * | 2020-04-20 | 2022-08-08 | 대한민국 | Fermented Black Ginseng extract including alcoholic liver injury prevention functional ingredients and method for manufacturing it |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1355019A (en) * | 2000-11-24 | 2002-06-26 | 刘国荣 | Process for preparing nano-class Chinese medicine powder |
| KR20090080847A (en) * | 2008-01-22 | 2009-07-27 | 정현석 | Beverage Composition Having Nano-size Ginseng Powder and Preparing Method Thereof |
| KR20100079874A (en) * | 2008-12-31 | 2010-07-08 | 성금수 | Preparation method of fermented ginseng or fermented red ginseng with active ginsenoside heightening absroption rate using lactic acid bacteria |
-
2015
- 2015-11-27 KR KR1020150167186A patent/KR20170061959A/en active Search and Examination
- 2015-12-23 CN CN201510977922.2A patent/CN106806396A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1355019A (en) * | 2000-11-24 | 2002-06-26 | 刘国荣 | Process for preparing nano-class Chinese medicine powder |
| KR20090080847A (en) * | 2008-01-22 | 2009-07-27 | 정현석 | Beverage Composition Having Nano-size Ginseng Powder and Preparing Method Thereof |
| KR20100079874A (en) * | 2008-12-31 | 2010-07-08 | 성금수 | Preparation method of fermented ginseng or fermented red ginseng with active ginsenoside heightening absroption rate using lactic acid bacteria |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107779475A (en) * | 2017-09-30 | 2018-03-09 | 安徽利民生物科技股份有限公司 | A kind of extracting method of sponge gourd saponin(e |
| CN107550961A (en) * | 2017-10-24 | 2018-01-09 | 东方红（通化）生物医药股份有限公司 | A kind of method that microbe Rapid Fermentation prepares ginseng/American ginseng extract |
| CN107619847A (en) * | 2017-11-09 | 2018-01-23 | 宁波润沃生物科技有限公司 | A kind of method that biofermentation prepares panaxoside metabolin |
| CN110664853A (en) * | 2018-07-03 | 2020-01-10 | 延边可喜安生物科技有限公司 | Method for extracting red ginseng saponin |
| CN109043535A (en) * | 2018-07-26 | 2018-12-21 | 哈尔滨工业大学（威海） | A kind of American ginseng extract rich in rare ginsenoside |
| CN113631146A (en) * | 2019-06-14 | 2021-11-09 | 自然灵感之园有限公司 | Whole plant extract containing root fermented product of ginseng cultivated by cold wind and cosmetic composition containing the same |
| CN112335875A (en) * | 2019-08-09 | 2021-02-09 | 株式会社艾福瑞特 | Health functional composition with blood circulation improving effect |
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