CN106801012A - A kind of extraction element of rapid extraction vaginitis pathogen - Google Patents
A kind of extraction element of rapid extraction vaginitis pathogen Download PDFInfo
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- CN106801012A CN106801012A CN201611214320.2A CN201611214320A CN106801012A CN 106801012 A CN106801012 A CN 106801012A CN 201611214320 A CN201611214320 A CN 201611214320A CN 106801012 A CN106801012 A CN 106801012A
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- extraction
- storehouse
- extraction element
- surge bunker
- rapid
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
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Abstract
The invention discloses a kind of extraction element of rapid extraction vaginitis pathogen, including extraction element spiral cover（1）, enzyme reaction storehouse（2）, cracking storehouse（3）, surge bunker（4）, drip nozzle（5）.It is capable of achieving the extraction of list sample nucleic acid, and can be after sample have been extracted, directly it is added drop-wise in the detection reagents such as downstream PCR amplified reaction, isothermal amplification reactions, more convenient nucleic acid subsequent use, with efficiency high, fast speed, low cost, it is reproducible, do not need professional training, stability is strong the features such as.The present invention is applied to biometrics field.
Description
Technical field
The present invention relates to biometrics field, the extraction of more particularly to a kind of rapid extraction vaginitis pathogen is filled
Put.
Background technology
At present, on-site rapid inspection (point-of-care testing, POCT) meaning is that sampling location is carried out
, a kind of testing result is quickly obtained using portable analysers and matched reagent detection mode.And gene studies field
The detection to nucleic acid is all be unable to do without, and this key precondition is to extract nucleic acid.But the treatment technology such as hand of current nucleic acid extraction
Work method is extracted, and the method for extracting nucleic acid such as full-automatic extraction device can not promptly extract nucleic acid, it is impossible to enough meet bedside fast
The demand of speed detection.It is common to cause the vaginitis pathogen to have fungi, gram-positive bacteria, Gram-negative bacteria, trichomonad, mycoplasma
Deng, and the more difficult extraction nucleic acid of the simple processing method of eucaryon bacterium, so a kind of extraction that can simultaneously extract all pathogen
Device demand increasingly increases, so being badly in need of the device of the rapid extraction nucleic acid for being independent of instrument for the POCT inspections of pathogen.
The content of the invention
To solve the above problems, the present invention provides a kind of nucleic acid extraction of the multiple pathogens that can be realized and extract single sample
Device.
The present invention solves the technical scheme that is used of above-mentioned technical problem：
A kind of extraction element of rapid extraction vaginitis pathogen, including extraction element enzyme reaction storehouse（2）Body, cracks storehouse（3）,
Surge bunker（4）And drip nozzle（5）.
It is further used as the improvement of technical solution of the present invention, enzyme reaction storehouse（2）Body contains enzyme dry powder, cracks storehouse（3）Contain
Cracking pulvis, surge bunker（4）Contain neutralization buffer, drip nozzle（5）Inside there are one 0.5 Mm filter film layers.
The improvement of technical solution of the present invention is further used as, each warehouse wall is made of thin-walled inert plastic, be easy to outer
Conduct heat and apply pressure in portion.
The improvement of technical solution of the present invention is further used as, each warehouse is connected by spiral lamination.
It is further used as the improvement of technical solution of the present invention, enzyme reaction storehouse（2）, crack storehouse（3）, surge bunker（4）Bottom contains
There is impermeable film, before being not connected with, each warehouse can be used alone.
The improvement of technical solution of the present invention is further used as, storehouse is cracked（3）, surge bunker（4）Top screw thread edge contains point
Mucro goes out.
It is further used as the improvement of technical solution of the present invention, enzyme reaction storehouse（2）It is red, transparent, cracks storehouse（3）It is yellow
It is transparent, surge bunker（4）It is water white transparency.
It is further used as the improvement of technical solution of the present invention, enzyme reaction storehouse（2）Contain spiral cover in top（1）, can individually open.
It is further used as the improvement of technical solution of the present invention, enzyme reaction storehouse（2）Bottom thread diameter can only be with cracking
Storehouse（3）Top connects, and cracking orlop portion diameter of thread size can only be with surge bunker（4）Top connects.
Beneficial effects of the present invention：
1. this extraction element for extracting vaginitis pathogen realizes the extraction of single sample nucleic acid by the connection in plastic tube storehouse, extracts
Process does not need instrument to heat it, and just nucleic acid extraction can be carried out to sample merely with the human body centre of the palm or axillaty temperature.
2. connect easy, just can complete extraction process within 20 minutes.
3. operating personnel need not be just operable by professional training.
4. there is low cost, the features such as extraction time is quick, it is adaptable to bedside is detected.
Brief description of the drawings
Fig. 1 is apparatus of the present invention overall structure diagram.
Specific embodiment
The invention will be further described below in conjunction with the accompanying drawings：
The technique effect of design of the invention, concrete structure and generation is carried out below with reference to embodiment and accompanying drawing clear, complete
Site preparation is described, to be completely understood by the purpose of the present invention, feature and effect.Obviously, described embodiment is the present invention
A part of embodiment, rather than whole embodiments, based on embodiments of the invention, those skilled in the art is not paying wound
The other embodiment that the property made is obtained on the premise of working, belongs to the technology of the present invention scope.
Reference picture 1, it is of the invention for a kind of extraction element of rapid extraction vaginitis pathogen, including the enzyme of extraction element are anti-
Ying Cang（2）Body, cracks storehouse（3）, surge bunker（4）, and drip nozzle（5）.
This device for extracting single sample nucleic acid passes through enzyme reaction storehouse（2）The dissolved destruction to pathogen outer wall is realized, is passed through
Cracking storehouse（3）Realize the cracking to pathogen cells film, nucleic acid formation nucleic acid solution discharged, by surge bunker（4）To nucleic acid
Solution carries out the absorption of pH value neutralization and impurity, drip nozzle（5）Realize the filtration to solid impurity and control nucleic acid solution
The rate of outflow., by interconnecting the extraction realized to pathogen nucleic acid, efficient high, low cost, speed is fast, is not required to for each warehouse
The features such as wanting professional training to operate.
Used as the preferred embodiment of the present invention, extraction element position in storehouse is enzyme reaction storehouse using order（2）, cracking storehouse（3）、
Surge bunker（4）.It is released state that each position in storehouse is used preceding, is fitted together by interface thread when using.
As the preferred embodiment of the present invention, enzyme reaction storehouse（2）Built-in lysozyme, cytase dry powder, crack storehouse（3）It is interior
Contain cracking pulvis, surge bunker（4）It is contained within neutralization buffer.
As the preferred embodiment of the present invention, in enzyme reaction storehouse（2）It is interior to add pending liquid sample, tighten spiral cover
（1）, turn upside down 5 times and mix sample, it is placed in the palm of the hand 15 minutes or is placed in oxter and heats for 10 minutes.
As the preferred embodiment of the present invention, enzyme reaction storehouse（2）After the completion of reaction, cracking storehouse is used（3）With enzyme reaction
Storehouse（2）Connection, is screwed by screw thread, and storehouse is cracked during screwing（3）The sharp protrusion on top scratches enzyme reaction storehouse（2）Bottom
Film, flows liquid into cracking storehouse（3）.
As the preferred embodiment of the present invention, cracking storehouse of acutely turning upside down（3）10 times, make cracking storehouse（3）With liquid
Fully mix, stand 2 minutes.
As the preferred embodiment of the present invention, storehouse is cracked（3）After the completion of reaction, surge bunker is used（4）With cracking storehouse（3）
Connection, is screwed, surge bunker during screwing by screw thread（4）The sharp protrusion on top scratches cracking storehouse（3）Bottom thin film, makes
Liquid flows into surge bunker（4）.
Used as the preferred embodiment of the present invention, acutely turn upside down surge bunker（4）10 times, make cracking storehouse（3）With liquid
Fully mix, it is ensured that liquid is all in surge bunker（4）, stand 3 minutes.
Used as the preferred embodiment of the present invention, body connected vertically holds actuating pressure in cracking storehouse by finger
（3）, nucleic acid solution is through filter membrane from drip nozzle（5）Ooze.
Whole nucleic acid extraction flow terminates, the nucleic acid for having extracted immediately using or move to preservation under -20 DEG C of environment.
The present invention can brush the vaginitis pathogenic bacteria in the vaginal fluid sample for taking with rapid extraction cotton swab, uterine neck
（The fungies such as gardnerella vaginalis, Trichomonas vaginalis, candida albicans and bacterium）In DNA, be widely used in medical treatment, judicial, high
The fields such as school, quality surveillance, Reagent Company, Disease Control and Prevention Center, research institute.
Certainly, the invention is not limited to above-mentioned implementation method, and those of ordinary skill in the art are without prejudice to originally
Equivalent variations or replacement can be also made on the premise of spirit, these equivalent deformations or replacement are all contained in the application right
It is required that in limited range.
Claims (10)
1. a kind of extraction element of rapid extraction vaginitis pathogen, it is characterised in that：Enzyme reaction storehouse including extraction element
（2）, cracking storehouse（3）And surge bunker（4）, and it is connected to surge bunker（4）The drip nozzle (5) of bottom.
2. a kind of extraction element of rapid extraction vaginitis pathogen according to claim 1, it is characterised in that：Described
Enzyme reaction storehouse（2）Contain spiral cover in top（1）, film layer and screw thread are arranged at bottom, and storehouse is contained within lysozyme and cytase dry powder.
3. a kind of extraction element of rapid extraction vaginitis pathogen according to claim 1, it is characterised in that：It is described to split
Xie Cang（3）Top is contained screw thread and is sharply protruded, and screw thread and film are contained in bottom, and warehouse includes cracking pulvis.
4. according to claim 1 a kind of extraction element of rapid extraction vaginitis pathogen its, it is characterised in that：It is described
Surge bunker（4）Top is contained screw thread and is sharply protruded, and bottom is directly connected to drip nozzle（5）, include buffer solution.
5. a kind of according to claim 1, extraction element of rapid extraction vaginitis pathogen, it is characterised in that：Drip nozzle（5）
With surge bunker（4）Contain a metafiltration film in coupling part.
6. according to claim 2 ~ 3, a kind of extraction element extraction element of rapid extraction vaginitis pathogen, its feature
It is：Enzyme reaction storehouse（2）Lower thread and cracking storehouse（3）Top screw thread can crack storehouse mutually immediately（3）Top sharply protrudes
Enzyme reaction storehouse can be scratched（2）Bottom thin film.
7. according to claim 3 ~ 4, a kind of extraction element extraction element of rapid extraction vaginitis pathogen, its feature
It is：Cracking storehouse（3）Lower thread and surge bunker（4）Top screw thread can mutually immediately, surge bunker（4）Top sharply protrudes energy
Enough puncture cracking storehouse（3）Bottom thin film.
8. according to claim 1 and claim 4, a kind of extraction element extraction element of rapid extraction vaginitis pathogen,
It is characterized in that：The drip nozzle（5）Mouth diameter, ooze every drop of liquid volume be 5 microlitres.
9. a kind of extraction element of rapid extraction vaginitis pathogen according to claim 1, it is characterised in that：Enzyme reaction
Storehouse（2）Using red, transparent is soft, inert plastic, storehouse is cracked（3）Using yellow transparent is soft, inert plastic, surge bunker（4）Adopt
With colourless soft, inert plastic.
10. according to right 6 ~ 7, a kind of extraction element of rapid extraction vaginitis pathogen, it is characterised in that：Enzyme reaction
Storehouse（2）Screw thread can not be with surge bunker（4）Connection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611214320.2A CN106801012A (en) | 2016-12-26 | 2016-12-26 | A kind of extraction element of rapid extraction vaginitis pathogen |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611214320.2A CN106801012A (en) | 2016-12-26 | 2016-12-26 | A kind of extraction element of rapid extraction vaginitis pathogen |
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| Publication Number | Publication Date |
|---|---|
| CN106801012A true CN106801012A (en) | 2017-06-06 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201611214320.2A Pending CN106801012A (en) | 2016-12-26 | 2016-12-26 | A kind of extraction element of rapid extraction vaginitis pathogen |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107574165A (en) * | 2017-09-27 | 2018-01-12 | 广州和实生物技术有限公司 | A kind of method for extracting nucleic acid and the extraction element for realizing this method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1804602A (en) * | 2005-12-15 | 2006-07-19 | 卢麟麟 | Apparatus and method for quick detection of surface cleanness degree and microbe contamination |
| CN205115443U (en) * | 2015-09-22 | 2016-03-30 | 广州和实生物技术有限公司 | Collection draws, amplifys and detects integrated gene detection device |
| CN205710700U (en) * | 2016-03-24 | 2016-11-23 | 广东和信健康科技有限公司 | A kind of nucleic acid extraction purification integrated apparatus |
-
2016
- 2016-12-26 CN CN201611214320.2A patent/CN106801012A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1804602A (en) * | 2005-12-15 | 2006-07-19 | 卢麟麟 | Apparatus and method for quick detection of surface cleanness degree and microbe contamination |
| CN205115443U (en) * | 2015-09-22 | 2016-03-30 | 广州和实生物技术有限公司 | Collection draws, amplifys and detects integrated gene detection device |
| CN205710700U (en) * | 2016-03-24 | 2016-11-23 | 广东和信健康科技有限公司 | A kind of nucleic acid extraction purification integrated apparatus |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107574165A (en) * | 2017-09-27 | 2018-01-12 | 广州和实生物技术有限公司 | A kind of method for extracting nucleic acid and the extraction element for realizing this method |
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Application publication date: 20170606 |
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