CN205710700U - A kind of nucleic acid extraction purification integrated apparatus - Google Patents
A kind of nucleic acid extraction purification integrated apparatus Download PDFInfo
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- CN205710700U CN205710700U CN201620241429.4U CN201620241429U CN205710700U CN 205710700 U CN205710700 U CN 205710700U CN 201620241429 U CN201620241429 U CN 201620241429U CN 205710700 U CN205710700 U CN 205710700U
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- pipe
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- nucleic acid
- mouth
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Abstract
This utility model provides a kind of nucleic acid extraction purification integrated apparatus, is formed with gel tube by preserving pipe.This integrated apparatus can also be disassembled into independent preserving pipe and gel tube and be equipped with pipe cap and the pipe close of correspondence, prevents leakage during sample transport, makes sample collection and nucleic acid extraction purification to carry out under various circumstances.This nucleic acid extraction integrated apparatus can reach Sample preservation, nucleic acid extraction and the purpose of purification integration operation easily, reduces sample process and nucleic acid extraction and the step of purification, has advantage that is simple to operate, the most effective and that effectively reduce cross-contamination.
Description
Technical field
The invention belongs to clinical diagnosis field, be specifically related to a kind of nucleic acid extraction purification integrated apparatus, main uses is virion
The detection preservation of sample, the rapid extraction of nucleic acid and purification during outer diagnosis.
Background technology
The extraction purification of the nucleic acid in Clinical detection sample includes 3 steps such as sample collection and storage, nucleic acid extraction and nucleic acid purification
Suddenly.At present, conventional method for extracting nucleic acid has centrifugal column method and paramagnetic particle method.Both approaches be required for first using E.C. 3.4.21.64 and
Surfactant destroys cellularity as cell pyrolysis liquid and discharges nucleic acid entrance solution so that nucleic acid and adsorbed film or magnetic bead
In conjunction with, the foreign material such as albumen are removed afterwards with rinsing liquid, last eluent is by the Nucleic Acid Elution on adsorption column or magnetic bead.
Existing nucleic acid extraction purification needs to carry out in different centrifuge tubes, needs repeatedly to be centrifuged and shift, not only results in core
The loss of acid sample, also can cause cross-contamination.Common process steps repeats and loaded down with trivial details, spends the time long, is unfavorable for clinical fast
Speed detection, and the price of nucleic acid extraction kit is higher, and each sample needs 30-50 unit.Therefore, in clinical diagnosis
In for the bigger case of sample size be badly in need of a kind of simple to operate, the most effective and cheap nucleic acid extraction purification process or
Detection device.
Utility model content
The purpose of this utility model is to provide a kind of nucleic acid extraction purification integrated apparatus, have simple to operate, the most effectively and
Reduce the multiple advantages such as cross-contamination.
In order to reach above-mentioned purpose, this utility model adopts the technical scheme that
A kind of nucleic acid extraction purification integrated apparatus, including for detachable apparatus, by preserving pipe and gel tube forms；Described preservation
Pipe is integrally made up of body and the mouth of pipe, and the mouth of pipe of described preservation pipe is provided with external screw thread；Described gel tube comprises the mouth of pipe, body and goes out
Sample ozzle；The described mouth of pipe, described body and described go out sample ozzle connect successively, one-body molded；Set inside the described gel tube mouth of pipe
Having female thread, fixing thin slice is contained in the bottom of the mouth of pipe；The inside of described body is contained agarose gel the most successively, is fixed
Circle and thin film；Described go out sample ozzle wide at the top and narrow at the bottom；Described preservation is managed and the described gel tube outer spiral shell by the mouth of pipe of described preservation pipe
The female thread of stricture of vagina and the mouth of pipe of described gel tube mutually suits and links into an integrated entity.
Preferably, the described pipe that preserves also comprises pipe cap and sealing ring, and the top inside described pipe cap is provided with described sealing ring, described
Being provided with anti-skidding striped outside pipe cap, the inner side of described pipe cap is provided with female thread, described female thread and described preservation pipe mouth of pipe external screw thread
Mutually suit.
Preferably, described gel tube also comprises pipe close and sealing ring；Described pipe close is solid, and the top of described pipe close is striate region,
The bottom of described pipe close is threaded area, arranges described sealing ring between described striate region and described threaded area；The spiral shell of described threaded area
Stricture of vagina mutually suits with described gel tube mouth of pipe female thread.
Preferably, described integrated apparatus also comprises closing device, and described closing device is closed square, is connected to out sample ozzle
End, with described go out sample ozzle one-body molded；Described closing device can be broken disconnected when using described device.
Preferably, described integrated apparatus also includes a collecting pipe, and described collecting pipe is socketed in outside described gel tube.
Preferably, the described pipe that preserves preserves pipe, possibly together with prominent layer outside described preservation pipe, described prominent layer for soft polypropylene
With described preservation pipe mouth of pipe external screw thread side edge.
Preferably, described agarose gel is filled in described gel tube tubular body, covers described thin layer；Described thin layer position
In its bottom, described ring for fixing is in thin layer thin layer fixed above.
Preferably, described fixing thin slice there is aperture；The bottom of described closing device is additionally provided with groove, and described groove is semicircle.
Preferably, described gel tube is polyacrylate hydrogel pipe；Described gel tube nozzle diameter is more than described gel tube pipe diameter；
Described gel tube pipe diameter is more than described gel tube ozzle top end diameter.
Compared with prior art, a kind of nucleic acid extraction integrated apparatus provided by the utility model can be by Sample preservation, nucleic acid
Extract and purification one step completes, minimizing sample process and nucleic acid extraction and the step of purification, and simple to operate and energy maximum limit
Degree reduces the cross-contamination during pattern detection.
Accompanying drawing explanation
Fig. 1 is that the master of a preferred embodiment of a kind of nucleic acid extraction purification integrated apparatus of the present utility model shows depending on tangent plane structure
Being intended to, Fig. 1-A is front view, and Fig. 1-B is left view sectional view；
Fig. 2 is the structural blast schematic diagram preserving pipe in embodiment 1；
Fig. 3 is the front view preserving pipe in embodiment 1 and sectional view, and Fig. 3-A is front view, and Fig. 3-B is left view sectional view；
Fig. 4 is the structural blast schematic diagram of the gel tube in embodiment 1；
Fig. 5 is front view and the sectional view of the gel tube in embodiment 1, and Fig. 5-A is front view, and Fig. 5-B is left view sectional view；
Accompanying drawing marks: 1, preserve pipe；11, the body of pipe is preserved；111, cell pyrolysis liquid；12, the mouth of pipe of pipe is preserved；13、
Preserve the external screw thread of the mouth of pipe of pipe；14, pipe cap；141, anti-skidding striped；142, pipe cap female thread；15, sealing ring I；16、
Prominent layer；2, gel tube；21, the gel tube mouth of pipe；211, the female thread of the mouth of pipe of gel tube；212, fixing thin slice；2121、
Aperture；22, body；221, polydextran gel layer；222, ring for fixing；223, thin layer；23, sample ozzle is gone out；24, envelope
Mouth device；241, groove；25, pipe close；251, striate region；252, threaded area；253, sealing ring II.
Detailed description of the invention
Below by specific embodiments and the drawings, this utility model is described further.
Fig. 1 is that the master of a preferred embodiment of a kind of nucleic acid extraction purification integrated apparatus of the present utility model shows depending on tangent plane structure
Being intended to, Fig. 1-A is front view, and Fig. 1-B is left view sectional view；From Fig. 1-A and Fig. 1-B, the one of this preferred embodiment
Body makeup is set to detachable apparatus, is made up of preservation pipe 1 and gel tube 2；Preserve pipe 1 and gel tube 2 by preserving the pipe of pipe
The external screw thread 13 of mouth mutually suits with the female thread 211 of the mouth of pipe of gel tube and links into an integrated entity.
From Fig. 1-A, preserving pipe 1 and be integrally made up of body 11 and the mouth of pipe 12, the mouth of pipe 12 preserving pipe is provided with external screw thread
13；Gel tube 2 comprises the mouth of pipe 21, body 22 and goes out sample ozzle 23；The mouth of pipe 21, body 22 and go out sample ozzle 23 phase successively
Connect, one-body molded.Preserve outside pipe 1 possibly together with prominent layer 16, prominent layer 16 and preservation pipe mouth of pipe external screw thread 13 side edge.
In the present embodiment, preserve pipe 1 and preserve pipe for soft polypropylene.The gel tube 2 of the present embodiment is polyacrylate hydrogel pipe；Gel
The pipe mouth of pipe 21 diameter is more than gel tube body 22 diameter；It is straight that gel tube body 22 diameter goes out sample ozzle 23 top more than gel tube
Footpath.
From Fig. 1-B, being provided with the female thread 211 of the mouth of pipe of gel tube inside the gel tube mouth of pipe 21, the bottom of the mouth of pipe 21 sets
There is fixing thin slice 212；The inside of gel tube body 22 is from top to bottom sequentially provided with polydextran gel layer 221, ring for fixing 222 and
Thin layer 223.It is internal that polydextran gel layer 221 is filled in described gel tube body 22, cover layer 223；Thin layer 223
Being positioned at bottom body 22, ring for fixing 222 is in thin layer 223 thin layer fixed above 223.
From Fig. 1-A and Fig. 1-B, it is wide at the top and narrow at the bottom that gel tube goes out sample ozzle 23.The present embodiment integrated apparatus also comprises sealing
Device 24, closing device 24 is closed square, is connected to out the end of sample ozzle 23, and sample ozzle 23 is one-body molded with going out；
Closing device 24 can be broken disconnected when using this device.
A kind of nucleic acid extraction purification integrated apparatus of the present utility model is detachable apparatus, when collecting experiment sample, preserves pipe 1
Can take apart with gel tube 2, independently deposit, convenient transport.
Fig. 2 is the structural blast schematic diagram preserving pipe in embodiment 1；During from Figure 2 it can be seen that preservation pipe 1 is used alone, also
Comprising pipe cap 14 and sealing ring 15, the top inside pipe cap 14 is provided with sealing ring I 15, and sealing is good, can avoid preserving
Sample liquid seepage in pipe；Being provided with anti-skidding striped 141 outside pipe cap 14, the inner side of pipe cap 14 is provided with female thread 142, female thread
142 mutually suit with the external screw thread 13 preserving the pipe mouth of pipe 12.Preserve outside pipe 1 possibly together with prominent layer 16, prominent layer 16 and guarantor
Deposit pipe mouth of pipe external screw thread 13 side edge.
Fig. 3 is the front view preserving pipe in embodiment 1 and sectional view, and Fig. 3-A is front view, and Fig. 3-B is left view sectional view；
From Fig. 3-A, outside pipe cap 14, it is provided with anti-skidding striped 141.From Fig. 3-B, the inner side of pipe cap 14 is provided with female thread
142, female thread 142 and the external screw thread 13 preserving the pipe mouth of pipe 12 mutually suit.
Fig. 4 is the structural blast schematic diagram of the gel tube in embodiment 1；From fig. 4, it can be seen that gel tube 2 also comprises pipe close 25 He
Sealing ring II253；Pipe close 25 is solid, and the top of pipe close 25 is striate region 251, and the bottom of pipe close 25 is threaded area 252,
Sealing ring 253 is set between striate region 251 and threaded area 252.The present embodiment integrated apparatus also comprises closing device 24, envelope
Mouth device 24 is closed square, is connected to out the end of sample ozzle 23, and sample ozzle 23 is one-body molded with going out；Closing device 24
Can break disconnected when using this device.
Fig. 5 is front view and the sectional view of the gel tube in embodiment 1, and Fig. 5-A is front view, and Fig. 5-B is left view sectional view.
From Fig. 5-A, gel tube 2 comprises the mouth of pipe 21, body 22 and goes out sample ozzle 23；The mouth of pipe 21, body 22 and go out sample pipe
Mouth 23 connects successively, one-body molded.Also comprising closing device 24, closing device 24 is closed square, is connected to out sample ozzle
The end of 23, sample ozzle 23 is one-body molded with going out；During carrying out nucleic acid purification, can by breaking disconnected closing device 24,
Sample after purification is made to flow out smoothly from going out sample ozzle 23, then, it is also possible to be enclosed within outside gel tube with a collecting pipe and receive sample.
Closing device 24 can be broken disconnected when using this device.The bottom of closing device 24 is additionally provided with groove 241, described groove 241
For semicircle.From Fig. 5-B, the screw thread of threaded area 252 mutually suits with gel tube mouth of pipe female thread 211.Fixing thin slice
There is aperture 2121 on 212, both made sample liquid can enter body 22, can prevent again the sample liquid in body 22 and glucosan from coagulating
Glue-line 221 does not outflows.It is internal that polydextran gel layer 221 is filled in described gel tube body 22, cover layer 223；Thin film
Layer 223 is positioned at bottom body 22, and ring for fixing 222 is in thin layer 223 thin layer fixed above 223.
Using a kind of nucleic acid extraction purification integrated apparatus provided by the utility model when, first gather such as bacterium solution, pharynx and wipe
The detection sample containing cell such as son, throat swab, blood, saliva, puts in preservation pipe 1 and tighten pipe cap 14, delivers to inspection
Room；Unscrew gel tube pipe close 25, make gel tube 2 separate with pipe close 25, then unscrew pipe cap 14, will preserve outside the pipe mouth of pipe
Screw thread 13 mutually suits with the female thread 221 of the gel tube mouth of pipe, tightens；Breaking disconnected closing device 24, extruding preserves pipe body 11
After making sample liquid enter gel tube body 2 and flow through polydextran gel 221 and thin film 223, certainly go out sample ozzle 23 and flow out, collect
3rd and the 4th sample carry out PCR detection.
A kind of nucleic acid extraction purification integrated apparatus provided by the utility model can also so use: cell cracking put into by sample
In liquid, vortex mixes, and places after 10min, takes mixed solution 50 μ L in gel tube；Break disconnected closing device, by gel tube
Insert in collecting pipe, by centrifuge, collect the centrifugal liquid obtained, then carry out PCR detection.
Claims (8)
1. a nucleic acid extraction purification integrated apparatus, it is characterised in that described integrated apparatus is detachable apparatus, is formed with gel tube by preserving pipe；
Described preservation pipe is integrally made up of body and the mouth of pipe, and the mouth of pipe of described preservation pipe is provided with external screw thread；
Described gel tube comprises the mouth of pipe, body and goes out sample ozzle；The described mouth of pipe, described body and described go out sample ozzle connect successively, one-body molded；
Being provided with female thread inside the described gel tube mouth of pipe, the bottom of the mouth of pipe is provided with fixing thin slice；
The inside of described gel tube body is from top to bottom sequentially provided with polydextran gel layer, ring for fixing and thin layer；
Described go out sample ozzle wide at the top and narrow at the bottom；
The described pipe that preserves mutually is suited with the female thread of the mouth of pipe of described gel tube by the external screw thread of the mouth of pipe of described preservation pipe with described gel tube and links into an integrated entity.
A kind of nucleic acid extraction purification integrated apparatus the most according to claim 1, it is characterised in that
The described pipe that preserves also comprises pipe cap and sealing ring I, and the top inside described pipe cap is provided with described sealing ring I, is provided with anti-skidding striped outside described pipe cap, and the inner side of described pipe cap is provided with female thread, and described female thread mutually suits with described preservation pipe mouth of pipe external screw thread；
Described gel tube also comprises pipe close and sealing ring II；Described pipe close is solid, and the top of described pipe close is striate region, and the bottom of described pipe close is threaded area, arranges described sealing ring II between described striate region and described threaded area；The screw thread of described threaded area mutually suits with described gel tube mouth of pipe female thread.
A kind of nucleic acid extraction purification integrated apparatus the most according to claim 1, it is characterised in that described integrated apparatus also comprises closing device, and described closing device is closed square, is connected to out sample ozzle end, with described go out sample ozzle one-body molded；Described closing device can be broken disconnected when using described device.
A kind of nucleic acid extraction purification integrated apparatus the most according to claim 1, it is characterised in that described integrated apparatus also includes a collecting pipe, and described collecting pipe is socketed in outside described gel tube.
A kind of nucleic acid extraction purification integrated apparatus the most according to claim 1, it is characterised in that described preservation pipe is that soft polypropylene preserves pipe, possibly together with prominent layer outside described preservation pipe, described prominent layer and described preservation pipe mouth of pipe external screw thread side edge.
A kind of nucleic acid extraction purification integrated apparatus the most according to claim 1, it is characterised in that described polydextran gel layer is filled in described gel tube tubular body, covers described thin layer；Described thin layer is positioned at its bottom, and described ring for fixing is positioned at thin layer thin layer fixed above.
A kind of nucleic acid extraction purification integrated apparatus the most according to claim 3, it is characterised in that have aperture on described fixing thin slice；The bottom of described closing device is additionally provided with groove, and described groove is semicircle.
A kind of nucleic acid extraction purification integrated apparatus the most according to claim 1, it is characterised in that described gel tube is polyacrylate hydrogel pipe；Described gel tube nozzle diameter is more than described gel tube pipe diameter；Described gel tube pipe diameter is more than described gel tube ozzle top end diameter.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620241429.4U CN205710700U (en) | 2016-03-24 | 2016-03-24 | A kind of nucleic acid extraction purification integrated apparatus |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620241429.4U CN205710700U (en) | 2016-03-24 | 2016-03-24 | A kind of nucleic acid extraction purification integrated apparatus |
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| CN205710700U true CN205710700U (en) | 2016-11-23 |
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| CN201620241429.4U Active CN205710700U (en) | 2016-03-24 | 2016-03-24 | A kind of nucleic acid extraction purification integrated apparatus |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106801012A (en) * | 2016-12-26 | 2017-06-06 | 广州和实生物技术有限公司 | A kind of extraction element of rapid extraction vaginitis pathogen |
| CN107815392A (en) * | 2017-11-10 | 2018-03-20 | 广州和实生物技术有限公司 | A kind of simple nucleic acid-extracting apparatus |
| CN109142012A (en) * | 2018-08-17 | 2019-01-04 | 上海迈景纳米科技有限公司 | A kind of FFPE sample fast dewaxing device and its application method |
-
2016
- 2016-03-24 CN CN201620241429.4U patent/CN205710700U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106801012A (en) * | 2016-12-26 | 2017-06-06 | 广州和实生物技术有限公司 | A kind of extraction element of rapid extraction vaginitis pathogen |
| CN107815392A (en) * | 2017-11-10 | 2018-03-20 | 广州和实生物技术有限公司 | A kind of simple nucleic acid-extracting apparatus |
| CN109142012A (en) * | 2018-08-17 | 2019-01-04 | 上海迈景纳米科技有限公司 | A kind of FFPE sample fast dewaxing device and its application method |
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