CN106800797A - The extracting method of stem of noble dendrobium pigment - Google Patents
The extracting method of stem of noble dendrobium pigment Download PDFInfo
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- CN106800797A CN106800797A CN201710064689.8A CN201710064689A CN106800797A CN 106800797 A CN106800797 A CN 106800797A CN 201710064689 A CN201710064689 A CN 201710064689A CN 106800797 A CN106800797 A CN 106800797A
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- pigment
- stem
- noble dendrobium
- filtrate
- ethanol
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B61/00—Dyes of natural origin prepared from natural sources, e.g. vegetable sources
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The extracting method of stem of noble dendrobium pigment, is related to Chinese medicine stem of noble dendrobium deep process technology field.The stem of noble dendrobium is crushed first, is subsequently added into 95% ethanol, be beaten using beater；Slurry is filtered, retains filtrate；To 95% ethanol is added in filter residue, heating and refluxing extraction is filtered again after cooling, and filter residue is stand-by, is merged filtrate twice and is obtained filtrate；Filtrate is centrifuged by centrifuge, is then stood and is obtained supernatant and precipitation；To vitamin C is added in supernatant, heating, vacuum negative pressure is concentrated into liquid extract, liquid extract is spray-dried, and finally crushing, grinding, sieving obtain pigment dry powder, to lactitol is added in pigment dry powder, is uniformly mixed acquisition stem of noble dendrobium pigment.Extracting method of the invention employs 95% ethanol and substitutes water, so that active ingredient can be discharged fully, so that extraction efficiency is higher, and can significantly lift the yield for extracting product.Than the extraction of the pigment of traditional pigment, yield more than 30% can be lifted.
Description
Technical field
The present invention relates to a kind of extracting method of stem of noble dendrobium pigment, it is related to Chinese medicine stem of noble dendrobium deep process technology field.
Background technology
The stem of noble dendrobium (scientific name：Dendrobium nobile Lindl), also known as celestial dry measure used in former times orchid rhythm, not dead grass, whorlleaf stonecrop herb, purple do not entangle celestial being
Strain, bracketplant, Lin Lan, prohibition of life, nobile flower etc..Stem is upright, and meat shape is plump, and slightly flat cylinder is long 10~60 centimetres, slightly reaches
1.3 centimetres.Medicinal plant, property slightly sweet flavor is micro- salty, trembles with fear, and returns stomach, kidney, lung channel.Reinforcing stomach reg fluid, nourishing Yin and clearing heat.For impairment of yin body fluid deficiency,
Dry polydipsia, deficiency of food is retched, after being ill abnormal heat, and mesh is secretly failed to understand.Stem of noble dendrobium flower appearance is graceful, and exquisite lovely, pattern is bright-coloured, fragrant odour,
It is called one of " four view and admire greatly foreign flower ".
Contain various alkaloids, polysaccharide, amino acid and starch etc. in stem of Dendrobium.Wherein dendrobium polysaccharide, alkaloid and phenol
The pharmacological action of the compounds such as class had not only been connected each other but also had had certain difference, and the pharmacological action of alkaloid is mainly manifested in anti-swelling
Knurl, to angiocardiopathy, the inhibitory action of enterogastric diseases and the analgesic effect of bringing down a fever etc.；Phenolic compound is mainly manifested in anti-ageing
Aspect of waiting for a long time effect；And the pharmacological action of dendrobium polysaccharide is mainly manifested in immune, hypoglycemic and anti-cancer, anticancer, anti-ageing waits for a long time
Aspect.
Dendrobium polysaccharide, is one of important component of the stem of noble dendrobium, is not the sugar with sugariness that we are recognized.It belongs to living
One kind of property polysaccharide, have functions that anti-inflammatory, it is antiviral, repair itself enhance immunity.The existing system for dendrobium polysaccharide
Preparation Method, the method for mainly using traditional water extraction, the maximum defect of this method is exactly most effective group in the stem of noble dendrobium
Divide to be dissolved in water completely, then cause the defects such as insufficient, the polysaccharide yield of extraction is low, processing cost is high.
Stem of noble dendrobium pigment, as a kind of emerging stem of noble dendrobium deep processing product, is with a wide range of applications.But, for stone
The extraction of dry measure used in former times pigment, reports that less, a small amount of document discloses the extracting method of stem of noble dendrobium pigment in the prior art, but, all deposit
In some more or less defects, for example, in pigment extraction process, yield is relatively low, medical value partial loss is more serious,
The colour component of preparation is more single, and the pot-life is shorter.
The content of the invention
An object of the present invention is to provide a kind of extracting method of stem of noble dendrobium pigment, and the extracting method technological design is closed
Reason, yield is higher.
To achieve the above object, present invention employs following technical scheme：
A kind of extracting method of stem of noble dendrobium pigment, step is as follows：
1., crushing and beating
The stem of noble dendrobium is crushed first, is subsequently added into 95% ethanol, be beaten using beater, the usage amount of the stem of noble dendrobium and ethanol
According to 1：10(g：ML) add；
2., filter
Slurry is filtered, retains filtrate a；To 95% ethanol for adding its 3 times of weight in filter residue a, it is heated to reflux carrying
Take, filtered again after cooling, filter residue b is stand-by, merge filtrate twice and obtain filtrate b；
3., it is centrifuged
Filtrate b is centrifuged by centrifuge, then 1 hour is stood, supernatant and precipitation is obtained；
4., the preparation of stem of noble dendrobium pigment
To vitamin C is added in supernatant, supernatant and ascorbic addition ratio are 1：1(L：G), heating, vacuum
Negative pressure is concentrated into liquid extract, and liquid extract is spray-dried, and finally crushes, grinds, crossing 100 mesh sieves acquisition pigment dry powder, to
Add lactitol in pigment dry powder, the weight ratio of pigment dry powder and lactitol is 10：1, it is uniformly mixed acquisition stem of noble dendrobium pigment.
Beneficial effects of the present invention are mainly manifested in：
1), extracting method employs 95% ethanol and substitutes traditional water, so that active ingredient can be discharged fully
Come, so that extraction efficiency is higher, and can significantly lift the yield for extracting product, than the extraction of traditional polysaccharide, can be lifted
Yield more than 80%；Than the extraction of the pigment of traditional pigment, yield more than 30% can be lifted.
2), the polysaccharide and pigment for preparing, component is different from traditional one-component, is compounded with product prepared by the present invention
Medicinal and additive component, so that the pot-life of product is longer, so that polysaccharide and pigment have more extensive using
On the way.
3), preparation technology is relatively simple, it is easy to which industrialized production, processing cost is low.
Specific embodiment
Below with reference to embodiment, the present invention is described in detail.But, embodiment content is only to this hair
Bright example and explanation, affiliated those skilled in the art do various repairing to described specific embodiment
Change or supplement or using similar mode substitute, without departing from invention design or surmount defined in the claims
Scope, all should belong to protection scope of the present invention.
Embodiment 1
A kind of extracting method of stem of noble dendrobium pigment and polysaccharide, step is as follows：
1., crushing and beating
The stem of noble dendrobium is crushed first, is subsequently added into 95% ethanol, be beaten using beater, the usage amount of the stem of noble dendrobium and ethanol
According to 1：10(g：ML) add.
2., filter
Slurry is filtered, retains filtrate a；To 95% ethanol for adding its 3 times of weight in filter residue a, it is heated to reflux carrying
Take, filtered again after cooling, filter residue b is stand-by, merge filtrate twice and obtain filtrate b.
3., it is centrifuged
Filtrate b is centrifuged by centrifuge, then 1 hour is stood, supernatant and precipitation is obtained.
4., the preparation of stem of noble dendrobium pigment
To vitamin C is added in supernatant, supernatant and ascorbic addition ratio are 1：1(L：G), heating, vacuum
Negative pressure is concentrated into liquid extract, and liquid extract is spray-dried, and finally crushes, grinds, crossing 100 mesh sieves acquisition pigment dry powder, to
Add lactitol in pigment dry powder, the weight ratio of pigment dry powder and lactitol is 10：1, it is uniformly mixed acquisition stem of noble dendrobium pigment.
5., the preparation of dendrobium polysaccharide
By filter residue b and precipitation according to 1：2 weight adds 10 times of distilled water of mixture weight than merging, and heats back
Stream 30 minutes, is then cooled to room temperature, stands 24 hours, is filtered to remove filtrate c, and filter cake is dried, and finally crushes, grinds
Mill, cross 100 mesh sieves and obtain polysaccharide dry powder, to xylitol is added in polysaccharide dry powder, the weight ratio of polysaccharide dry powder and xylitol is 20：
1, it is uniformly mixed acquisition dendrobium polysaccharide.
Claims (1)
1. a kind of extracting method of stem of noble dendrobium pigment, it is characterised in that：Step is as follows：
1., crushing and beating
The stem of noble dendrobium is crushed first, is subsequently added into 95% ethanol, be beaten using beater, the usage amount of the stem of noble dendrobium and ethanol according to
1：10(g：ML) add；
2., filter
Slurry is filtered, retains filtrate a；To 95% ethanol that its 3 times of weight are added in filter residue a, heating and refluxing extraction is cold
But filtered again after, filter residue b is stand-by, merge filtrate twice and obtain filtrate b；
3., it is centrifuged
Filtrate b is centrifuged by centrifuge, then 1 hour is stood, supernatant and precipitation is obtained；
4., the preparation of stem of noble dendrobium pigment
To vitamin C is added in supernatant, supernatant and ascorbic addition ratio are 1：1(L：G), heating, vacuum negative pressure
Liquid extract is concentrated into, liquid extract is spray-dried, finally crushed, grind, 100 mesh sieves acquisition pigment dry powder being crossed, to pigment
Add lactitol in dry powder, the weight ratio of pigment dry powder and lactitol is 10：1, it is uniformly mixed acquisition stem of noble dendrobium pigment.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201710064689.8A CN106800797A (en) | 2017-02-05 | 2017-02-05 | The extracting method of stem of noble dendrobium pigment |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710064689.8A CN106800797A (en) | 2017-02-05 | 2017-02-05 | The extracting method of stem of noble dendrobium pigment |
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| CN106800797A true CN106800797A (en) | 2017-06-06 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113856308A (en) * | 2021-10-28 | 2021-12-31 | 江苏先卓食品科技股份有限公司 | Purifier of lactitol production usefulness |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103910810A (en) * | 2014-04-16 | 2014-07-09 | 北京林业大学 | Method for simultaneously preparing dendrobium nobile pigments and dendrobium nobile polysaccharides by using dendrobium nobile raw materials |
| CN104628798A (en) * | 2015-01-27 | 2015-05-20 | 中国农业科学院农产品加工研究所 | Method for preparing anthocyanin and polysaccharide from raw material purple dendrobe |
-
2017
- 2017-02-05 CN CN201710064689.8A patent/CN106800797A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103910810A (en) * | 2014-04-16 | 2014-07-09 | 北京林业大学 | Method for simultaneously preparing dendrobium nobile pigments and dendrobium nobile polysaccharides by using dendrobium nobile raw materials |
| CN104628798A (en) * | 2015-01-27 | 2015-05-20 | 中国农业科学院农产品加工研究所 | Method for preparing anthocyanin and polysaccharide from raw material purple dendrobe |
Non-Patent Citations (3)
| Title |
|---|
| 杨晓娜等: "龙陵紫皮石斛色素的提取及其稳定性", 《食品科学》 * |
| 梁青青等: "野生铁皮石斛色素超声提取工艺的优选", 《微量元素与健康研究》 * |
| 王玲等: "石斛兰色素的提取及稳定性研究", 《林业科技》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113856308A (en) * | 2021-10-28 | 2021-12-31 | 江苏先卓食品科技股份有限公司 | Purifier of lactitol production usefulness |
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Application publication date: 20170606 |