CN106797966A - A kind of Chinese medicine antiseptic - Google Patents
A kind of Chinese medicine antiseptic Download PDFInfo
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- CN106797966A CN106797966A CN201710022659.0A CN201710022659A CN106797966A CN 106797966 A CN106797966 A CN 106797966A CN 201710022659 A CN201710022659 A CN 201710022659A CN 106797966 A CN106797966 A CN 106797966A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
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Abstract
The present invention relates to a kind of Chinese medicine antiseptic, it is characterised in that the volatile oil of volatile oil, the volatile oil that 100 1000g folium artemisiae argyis are extracted and the extraction of 5 100g cloves that every liter of disinfectant liquid is extracted by 100 1000g grass-leaved sweetflags is made.Chinese medicine antiseptic of the present invention, not only flavour of a drug are few, are conducive to quality control, and medicine letter power is specially, effect is significant.
Description
Invention field
The present invention relates to medicinal preparation, and in particular to contain the pharmaceutical preparation for not determining structure from plant, the medicine
Sterilization of the thing preparation suitable for living environment.
Technical background
With the development of society, environment has also suffered pollution, and the concentration of pollutant has met or exceeded detrimental extent, caused
The destruction of the ecosystem, the growth for virus, bacterium and fungi occur, causes harm to people.Find one kind quickly, safely, effectively,
And people will not be brought influence and discomfort disinfectant, as problem anxious to be resolved.
Ancients' every Dragon Boat Festival hangs over the custom on door and window with regard to useful fresh grass-leaved sweetflag, fresh folium artemisiae argyi, is used as prevention pestilence,
Also the Chinese medicines such as folium artemisiae argyi, grass-leaved sweetflag, cloves, the root of Dahurain angelica, dried orange peel, honeysuckle, wrinkled giant hyssop, perilla leaf, peppermint, Ligusticum wallichii, peppermint, lavender are commonly used
Sachet is made, this is all derived from this kind of Chinese medicine has functions that fragrance is warded off turbid, removing toxic substances and got rid of evils.Modern medicine shows, such Chinese medicine
There is different degrees of suppression or killing effect to bacterium and fungi.
The patent application of Publication No. CN104206451A discloses a kind of air sanitizer, the disinfectant by：Folium artemisiae argyi, plate
Lan Gen, grass-leaved sweetflag, kuh-seng, dandelion, cloves, Hangzhou of China volatile oil, odium stearate, glycerine, folium isatidis, Herba Moslae Chinensis, castor leaf
Etc. being made.The flavour of a drug of the medicine described in above-mentioned patent application are on the high side, it is difficult to control quality.
The content of the invention
Problem to be solved by this invention is to provide a kind of Chinese medicine antiseptic, and flavour of a drug contained by the Chinese medicine antiseptic are few, quality
Stabilization, effect is significant.
Technical proposal that the invention solves the above-mentioned problems is as described below：
A kind of Chinese medicine antiseptic, it is characterised in that the volatilization that every liter of disinfectant liquid is extracted by 100-1000g grass-leaved sweetflags
The volatile oil that the volatile oil and 5-100g cloves that oil, 100-1000g folium artemisiae argyis are extracted are extracted is made.
Preferably, described Chinese medicine antiseptic, volatile oil that every liter of disinfectant liquid is extracted by 500g grass-leaved sweetflags,
The volatile oil that the volatile oil and 10g cloves that 700g folium artemisiae argyis are extracted are extracted is made.
Above-mentioned Chinese medicine antiseptic, its disinfectant liquid can contain the ethanol with hydrotropy and the effect such as anti-oxidant.
Above-mentioned Chinese medicine antiseptic, its disinfectant liquid can also contain essence that can be fragrant, and the essence is lemonene
One or more in smart, refined blue essence and windbell essence.
Above-mentioned disinfectant is obtained by following methods：
The volatile oil of grass-leaved sweetflag extraction, the volatile oil of folium artemisiae argyi extraction, the volatile oil of cloves extraction, essence are well mixed,
Dissolving in ethanol, is added water and is settled to 1000ml, and product is obtained.
A kind of Chinese medicine antiseptic of the present invention has advantages below compared with prior art：
Chinese medicine antiseptic of the present invention is only made up of grass-leaved sweetflag, folium artemisiae argyi, cloves, and not only flavour of a drug are few, are conducive to quality
Control, and medicine letter power is specially, effect is significant.
The present invention is more fully understood for the ease of the public, beneficial effect of the invention is further illustrated below by experiment
Really.
First, test medicine：
Experimental group 1：The liquid of following embodiments one.
Experimental group 2：The liquid of following embodiments three.
Experimental group 3：The liquid of following embodiments five.
Control group 1：Remove the liquid of volatile argyi leaf oil and volatile clove oil in embodiment 1.
Control group 2：Remove the liquid of Essential Oil of Acorus tatarinowii and volatile clove oil in embodiment 1.
Control group 3：Remove the liquid of volatile argyi leaf oil and Essential Oil of Acorus tatarinowii in embodiment 1.
Control group 4：Remove the liquid of volatile clove oil in embodiment 1.
Control group 5：Remove the liquid of volatile argyi leaf oil in embodiment 1.
Control group 6：Remove the liquid of Essential Oil of Acorus tatarinowii in embodiment 1.
Control group 7：The medicine that prescription as described in embodiment 3 in the patent application of Publication No. CN104206451A is made
Liquid.
Control group 8：The original-pack portable thimerosals of domol of Germany.
Control group 9：Remove the liquid of volatile clove oil plus Hangzhou of China volatile oil in embodiment 1.The preparation method of the liquid
It is as follows：
Take 500g grass-leaved sweetflags and extract Essential Oil of Acorus tatarinowii, 700g folium artemisiae argyis extract volatile argyi leaf oil, and 10g FLOS CHRYSANTHEMI from Hangzhou of China extracts Hang Ju
Flowers volatile oil.By Essential Oil of Acorus tatarinowii, volatile argyi leaf oil, Hangzhou of China volatile oil and appropriate essence mixing, 700ml second is dissolved in
In alcohol, 1000ml is settled to water, product is obtained.
Control group 10：Remove the liquid of volatile clove oil plus dandelion water extract in embodiment 1.The preparation method of the liquid
It is as follows：
Take 500g grass-leaved sweetflags and extract Essential Oil of Acorus tatarinowii, 700g folium artemisiae argyis extract volatile argyi leaf oil, and it is public that 10g dandelions extract Pu
English water extract.By Essential Oil of Acorus tatarinowii, volatile argyi leaf oil, dandelion water extract and appropriate essence mixing, 700ml second is dissolved in
In alcohol, 1000ml is settled to water, product is obtained.The preparation method of wherein described dandelion water extract is：Dandelion is taken to add
Water is extracted 2 times, for the first time 8 times of amount water, and second 6 times of amount water, each 2h, merging filtrate stands overnight, and takes supernatant liquid filtering simultaneously
Medicinal extract is concentrated into, be dry, pulverize and obtained final product dandelion water extract.
Control group 11：Remove the liquid of volatile clove oil plus kuh-seng water extract in embodiment 1.The preparation method of the liquid is such as
Under：
Take 500g grass-leaved sweetflags and extract Essential Oil of Acorus tatarinowii, 700g folium artemisiae argyis extract volatile argyi leaf oil, and 10g kuh-sengs extract kuh-seng water
Extract.Essential Oil of Acorus tatarinowii, volatile argyi leaf oil, kuh-seng water extract and appropriate essence mixing are dissolved in 700ml ethanol,
1000ml is settled to water, product is obtained.The preparation method of wherein described kuh-seng water extract is：Take kuh-seng extracting in water 2 times,
8 times of amount water, measures water for 6 times for the second time for the first time, and each 2h, merging filtrate stands overnight, takes supernatant liquid filtering and be concentrated into leaching
Cream, dry, pulverize and obtain final product kuh-seng water extract.
Control group 12：Remove the liquid of volatile argyi leaf oil plus schizonepeta water extract in embodiment 1.The preparation method of the liquid is such as
Under：
Take 500g grass-leaved sweetflags and extract Essential Oil of Acorus tatarinowii, 10g cloves extracts volatile clove oil, and 700g schizonepeta extracts schizonepeta water
Extract.Essential Oil of Acorus tatarinowii, volatile clove oil, schizonepeta water extract and appropriate essence mixing are dissolved in 700ml ethanol,
1000ml is settled to water, product is obtained.The preparation method of wherein described schizonepeta water extract is：Take schizonepeta extracting in water 2 times,
8 times of amount water, measures water for 6 times for the second time for the first time, and each 2h, merging filtrate stands overnight, takes supernatant liquid filtering and be concentrated into leaching
Cream, dry, pulverize and obtain final product schizonepeta water extract.
Control group 13：Remove the liquid of volatile argyi leaf oil plus capsule of weeping forsythia water extract in embodiment 1.The preparation method of the liquid is such as
Under：
Take 500g grass-leaved sweetflags and extract Essential Oil of Acorus tatarinowii, 10g cloves extracts volatile clove oil, and the 700g capsules of weeping forsythia extract capsule of weeping forsythia water
Extract.Essential Oil of Acorus tatarinowii, volatile clove oil, capsule of weeping forsythia water extract and appropriate essence mixing are dissolved in 700ml ethanol,
1000ml is settled to water, product is obtained.The preparation method of wherein described capsule of weeping forsythia water extract is：Take capsule of weeping forsythia extracting in water 2 times,
8 times of amount water, measures water for 6 times for the second time for the first time, and each 2h, merging filtrate stands overnight, takes supernatant liquid filtering and be concentrated into leaching
Cream, dry, pulverize and obtain final product capsule of weeping forsythia water extract.
Control group 14：Remove the liquid of volatile argyi leaf oil plus effects of Radix Isatidis water extract in embodiment 1.The preparation method of the liquid
It is as follows：
Take 500g grass-leaved sweetflags and extract Essential Oil of Acorus tatarinowii, 10g cloves extracts volatile clove oil, and it is blue that 700g Radix Isatidis extracts plate
Root water extract.By Essential Oil of Acorus tatarinowii, volatile clove oil, effects of Radix Isatidis water extract and appropriate essence mixing, 700ml second is dissolved in
In alcohol, 1000ml is settled to water, product is obtained.The preparation method of wherein described effects of Radix Isatidis water extract is：Radix Isatidis is taken to add
Water is extracted 2 times, for the first time 8 times of amount water, and second 6 times of amount water, each 2h, merging filtrate stands overnight, and takes supernatant liquid filtering simultaneously
Medicinal extract is concentrated into, be dry, pulverize and obtained final product Radix Isatidis water extract.
Control group 15：Remove the liquid of Essential Oil of Acorus tatarinowii plus rhizome of cyrtomium water extract in embodiment 1.The preparation method of the liquid
It is as follows：
Take 700g folium artemisiae argyis and extract volatile argyi leaf oil, 10g cloves extracts volatile clove oil, and 500g rhizome of cyrtomium extracts rhizome of cyrtomium water extraction
Thing.Volatile argyi leaf oil, volatile clove oil, rhizome of cyrtomium water extract and appropriate essence mixing are dissolved in 700ml ethanol, use water
1000ml is settled to, product is obtained.The preparation method of wherein described rhizome of cyrtomium water extract is：Take rhizome of cyrtomium extracting in water 2 times, first
Secondary 8 times of amounts water, second 6 times of amount water, each 2h, merging filtrate stands overnight, takes supernatant liquid filtering and be concentrated into medicinal extract, does
Dry, crushing obtains final product rhizome of cyrtomium water extract.
Control group 16：Remove the liquid of Essential Oil of Acorus tatarinowii plus coptis water extract in embodiment 1.The preparation method of the liquid
It is as follows：
Take 700g folium artemisiae argyis and extract volatile argyi leaf oil, 10g cloves extracts volatile clove oil, and the 500g coptiss extract coptis water extraction
Thing.Volatile argyi leaf oil, volatile clove oil, coptis water extract and appropriate essence mixing are dissolved in 700ml ethanol, use water
1000ml is settled to, product is obtained.The preparation method of wherein described coptis water extract is：Take coptis extracting in water 2 times, first
Secondary 8 times of amounts water, second 6 times of amount water, each 2h, merging filtrate stands overnight, takes supernatant liquid filtering and be concentrated into medicinal extract, does
Dry, crushing obtains final product coptis root aqueous extract.
Control group 17：Remove the liquid that Essential Oil of Acorus tatarinowii increases leafiness water extract in embodiment 1.The preparation side of the liquid
Method is as follows：
Take 700g folium artemisiae argyis and extract volatile argyi leaf oil, 10g cloves extracts volatile clove oil, and 500g folium isatidis extracts folium isatidis water
Extract.Volatile argyi leaf oil, volatile clove oil, folium isatidis water extract and appropriate essence mixing are dissolved in 700ml ethanol,
1000ml is settled to water, product is obtained.The preparation method of wherein described folium isatidis water extract is：Take folium isatidis extracting in water
2 times, 8 times of amount water, measures water for 6 times for the second time for the first time, and each 2h, merging filtrate stands overnight, takes supernatant liquid filtering and be concentrated into
Medicinal extract, dry, pulverize and obtain final product folium isatidis water extract.
2nd, extracorporeal bacteria inhibitor test:
Under aseptic technique, the ring of each bacterial strain one is taken respectively with different vaccination ring, be seeded in blood respectively with method of scoring and put down
In plate, chocolate flat board Czapek's medium, Martin's culture medium and sabouraud culture medium, bacterium cultivates 24 in putting 37 DEG C of constant incubators
Hour, taking-up is saved backup in being put into 4 DEG C of refrigerators after 24 hours；Fungi is incubated 2 weeks on sabouraud culture medium inclined-plane, is chosen with inoculation hook
Appropriate bacterium colony (about 1 × 1mm) is taken to be put into sterilization mortar.When using, each strain prepares bacteria suspension with normal saline dilution respectively,
The ultimate density for adjusting each bacteria suspension with Hematometer tally method is 5 × 105CFU/ml。
Using Tubes serial dilution method, 7, test tube (the 1st medicine of cuvette cartridge centrifugation equipped with 1ml nutrient broths is taken respectively
Thing supernatant 1ml), it is arranged on rack for test tube, number, meat soup 1ml is added in the 1st test tube, shake up, draw 1ml and add the 2nd pipe
In, shake up, the 7th test tube is diluted to method, 1ml is discarded, the 1st to the 7th test tube pastille supernatant concentration is respectively 25%,
12.5%th, 6.25%, 3.125%, 1.56%, 0.78%, 0.39%.Another standby 1 test tube is as blank tube.
With pipettor in each test tube be inoculated with each corresponding bacteria suspensions of 1ml, be respectively staphylococcus aureus, Pseudomonas aeruginosa,
Escherichia coli, bacillus subtilis, micrococcus scarlatinae, corynebacteria, mould, epidermis floccus mentagrophyte, aspergillus niger are (by Guangzhou
Medical pharmaceutical university microorganism teaching and research room provides), negative control (nutrient broth pipe) and positive control (strain control tube) are set in addition,
Staphylococcus aureus, 37 DEG C of Escherichia coli are incubated 4h observation results, Pseudomonas aeruginosa, bacillus subtilis, micrococcus scarlatinae
37 DEG C are incubated 18h observation results with corynebacteria, and 37 DEG C of incubation 6d of mould and aspergillus niger, epidermis floccus mentagrophyte is incubated 12d.Daily
Observation 1 time, 6d observations result, has no the invisible spectro minimum drug concentration as MIC of bacterial growth after inoculation.
Each group antibacterial experiment in vitro the results are shown in Table 1 and 2.
The each group antibacterial experiment in vitro result of table 1
The each group antibacterial experiment in vitro result of table 2
Above-mentioned experiment shows that experimental group of the present invention can be to staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, withered grass bud
The fungi such as the bacteriums such as spore bacillus, micrococcus scarlatinae, corynebacteria and mould, epidermis floccus mentagrophyte, aspergillus niger has inhibitory action.
Compared with control group, experimental group plays the role of to strengthen or has equal suppression bacterium and fungi, and experimental group 1 is preferred scheme.
3rd, sterilizing rate experiment：
The liquid 10ml and sterilized water 10ml of experimental group 1 are taken, in the fine taper bottle being placed in after sterilizing, bottle stopper is stoppered, is taken and is matched somebody with somebody
Good corresponding bacteria suspension, is added dropwise 100 μ l bacteria suspensions in the liquid and sterilized water of experimental group 1 respectively, starts timing, in oscillator
After the upper effect of concussion respectively 10min, (0.5% is thio to draw 0.5ml liquid input nertralizer containing 10ml respectively with aseptic liquid-transfering gun
Sodium sulphate, 2.0% Tween-80, the 0.03mol/LPBS (phosphate buffer) of 0.2% lecithin) aseptic fine taper bottle in,
Fully mix, do appropriate dilution, then take wherein 3 dilution factors, 0.5ml is drawn respectively, be placed in 3 plates, with it is cool to 40~
45 DEG C of nutrient agar (bacterium) or sabouraud's agar (fungi) 15ml is poured into, and rotates plate, makes its abundant
Uniformly, flat board, 37 DEG C of cultures 48h (bacterium) or 72h (fungi) are overturn after agar solidification, makees viable bacteria colony counting, experiment with computing
Sterilizing rate of the liquid of group 1 to various bacterium.Experiment is repeated 3 times, and is calculated as follows sterilizing rate：Sterilizing rate (%)=(sterilized water is made
With the liquid effects average colony number of average colony number-experimental group 1) liquid effects average colony number × 100% of/experimental group 1.
Each group extracorporeal disinfecting rate experimental result is shown in Table 3 and 4.
Result of each group liquid of table 3 to the sterilizing rate of various bacterium
Result of each group liquid of table 4 to the sterilizing rate of various bacterium
Statistical result showed, compares with experimental group 1, * P<0.05.
Above-mentioned experimental result shows, Disinfection Effect meets execution from 1 day July in 2002《Regulations of sterilization management》.
Statistical procedures
Total data carries out statistical analysis, measurement data mean ± standard deviation (x using the statistics softwares of SPSS 19.0
± s) represent.
Specific embodiment
Essential Oil of Acorus tatarinowii, volatile argyi leaf oil and volatile clove oil use conventional method during below prepared by five embodiments
Extract.
Embodiment one
1st, prescription：By weight, 500g grass-leaved sweetflags are taken and extract Essential Oil of Acorus tatarinowii, 700g folium artemisiae argyis extract volatile argyi leaf oil,
10g cloves extracts volatile clove oil.
2nd, preparation method：By Essential Oil of Acorus tatarinowii, volatile argyi leaf oil and volatile clove oil and appropriate lemon extract mix,
It is dissolved in 700ml ethanol, 1000ml is settled to water, product is obtained.
Embodiment two
1st, prescription：By weight, 110g grass-leaved sweetflags are taken and extract Essential Oil of Acorus tatarinowii, 1000g folium artemisiae argyis extract volatile argyi leaf oil,
100g cloves extracts volatile clove oil.
2nd, preparation method：By Essential Oil of Acorus tatarinowii, volatile argyi leaf oil, volatile clove oil and appropriate lemon extract and Ya Lan
Essence mixes, and is dissolved in 700ml ethanol, and 1000ml is settled to water, and product is obtained.
Embodiment three
1st, prescription：By weight, 700g grass-leaved sweetflags are taken and extract Essential Oil of Acorus tatarinowii, 360g folium artemisiae argyis extract volatile argyi leaf oil,
50g cloves extracts volatile clove oil.
2nd, preparation method：By Essential Oil of Acorus tatarinowii, volatile argyi leaf oil, volatile clove oil and appropriate lemon extract and Ya Lan
Essence mixes, and is dissolved in 700ml ethanol, and 1000ml is settled to water, and product is obtained.
Example IV
1st, prescription：By weight, 1000g grass-leaved sweetflags are taken and extract Essential Oil of Acorus tatarinowii, 190g folium artemisiae argyis extract volatile argyi leaf oil,
20g cloves extracts volatile clove oil.
2nd, preparation method：By Essential Oil of Acorus tatarinowii, volatile argyi leaf oil, volatile clove oil and appropriate lemon extract and Ya Lan
Essence mixes, and is dissolved in 700ml ethanol, and 1000ml is settled to water, and product is obtained.
Embodiment five
1st, prescription：By weight, 300g grass-leaved sweetflags are taken and extract Essential Oil of Acorus tatarinowii, 810g folium artemisiae argyis extract volatile argyi leaf oil,
100g cloves extracts volatile clove oil.
2nd, preparation method：By Essential Oil of Acorus tatarinowii, volatile argyi leaf oil, volatile clove oil and appropriate lemon extract, windbell
Essence and Ya Lan essence mix, and are dissolved in 700ml ethanol, and 1000ml is settled to water, and product is obtained.
Claims (4)
1. a kind of Chinese medicine antiseptic, it is characterised in that volatile oil that every liter of disinfectant liquid is extracted by 100-1000g grass-leaved sweetflags,
The volatile oil that the volatile oil and 5-100g cloves that 100-1000g folium artemisiae argyis are extracted are extracted is made.
2. a kind of Chinese medicine antiseptic according to claim 1, it is characterised in that every liter of disinfectant liquid is by 500g grass-leaved sweetflags
The volatile oil that the volatile oil and 10g cloves that the volatile oil of extraction, 700g folium artemisiae argyis are extracted are extracted is made.
3. a kind of Chinese medicine antiseptic according to claim 1 and 2, it is characterised in that also contain in described disinfectant liquid
There is ethanol.
4. a kind of Chinese medicine antiseptic according to claim 3, it is characterised in that also contain perfume in described disinfectant liquid
Essence, the essence be lemon extract, refined blue essence and windbell essence in one or more.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710022659.0A CN106797966A (en) | 2017-01-12 | 2017-01-12 | A kind of Chinese medicine antiseptic |
| CN201710504045.6A CN107197881A (en) | 2017-01-12 | 2017-06-28 | A kind of Chinese medicine antiseptic |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710022659.0A CN106797966A (en) | 2017-01-12 | 2017-01-12 | A kind of Chinese medicine antiseptic |
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| CN201710504045.6A Pending CN107197881A (en) | 2017-01-12 | 2017-06-28 | A kind of Chinese medicine antiseptic |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102258063A (en) * | 2011-04-28 | 2011-11-30 | 张志明 | Chinese medicinal air freshening bactericide |
| CN103585540B (en) * | 2013-08-31 | 2015-06-17 | 云南中医学院 | Incense-fumigating oil of rhizoma atractylodis and folium artemisiae argyi |
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Application publication date: 20170606 |