CN106754771A - The extracting method of polyphenol oxidase in a kind of lychee exocarp - Google Patents
The extracting method of polyphenol oxidase in a kind of lychee exocarp Download PDFInfo
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- CN106754771A CN106754771A CN201611144724.9A CN201611144724A CN106754771A CN 106754771 A CN106754771 A CN 106754771A CN 201611144724 A CN201611144724 A CN 201611144724A CN 106754771 A CN106754771 A CN 106754771A
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- Prior art keywords
- polyphenol oxidase
- extracting method
- lychee exocarp
- drying
- powder
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0055—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10)
- C12N9/0057—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10) with oxygen as acceptor (1.10.3)
- C12N9/0059—Catechol oxidase (1.10.3.1), i.e. tyrosinase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y110/00—Oxidoreductases acting on diphenols and related substances as donors (1.10)
- C12Y110/03—Oxidoreductases acting on diphenols and related substances as donors (1.10) with an oxygen as acceptor (1.10.3)
- C12Y110/03001—Catechol oxidase (1.10.3.1), i.e. tyrosinase
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- Wood Science & Technology (AREA)
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- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
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- Biotechnology (AREA)
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- Coloring Foods And Improving Nutritive Qualities (AREA)
- Extraction Or Liquid Replacement (AREA)
Abstract
The present invention provides a kind of extracting method of polyphenol oxidase in lychee exocarp, belongs to technical field of biological extraction.The technology of the present invention is used after the drying of fresh lichee shell is clayed into power, and by the extraction of crude enzyme liquid, after ultrasonication, is concentrated and is obtained finished product after freeze-dried.Extractive technique of the invention, process is simple, rationally, gained polyphenol oxidase can be widely used for medicine, food and association area to flow.
Description
Technical field
The present invention relates to technical field of biological extraction, and in particular to the extracting method of polyphenol oxidase in a kind of lychee exocarp.
Background technology
Lichee, originates in SOUTHERN CHINA, is subtropical fruit tree, and aiphyllium is high about 10 meters, with banana, pineapple, longan one
It is same to be known as " the big fruit in the southern part of the country four ".Litchi meat is sweet, sour, warm in nature, enters the heart, spleen, Liver Channel；Hiccup can be stopped, only suffered from diarrhoea, be intractable
Hiccup and the diet good food of diarrhea before dawn person, while there is benefit brain and enhancing fitness, the beneficial spleen of appetizing has the effect of to promote appetite.The pericarp of lichee
There is fish-scale shape projection, scarlet, purplish red, lychee exocarp is bitter in taste, tremble with fear, enter the heart channel of Hang-Shaoyin；Its chemical composition is mainly polyphenol oxidase, has
Qi-regulating, relieve pain, promote the production of body fluid, beneficial blood, controlling polydipsia, inducing astrigency effect, curing mainly dysentery, metrorrhagia, eczema；Stomachache, toothache, traumatism and bleeding；Stomach
Gastral cavity pain, hernia pain, women's pricking caused by the stagnation of QI and blood etc., have broad application prospects.Therefore, it is necessary to work out one kind can effectively carry
The method for taking polyphenol oxidase in lychee exocarp.
The content of the invention
Goal of the invention of the invention is：For above-mentioned problem, there is provided polyphenol oxidase in a kind of lychee exocarp
Extracting method, the method is used after the drying of fresh lichee shell is clayed into power, by the extraction of crude enzyme liquid, through ultrasonication
Afterwards, concentrate and obtain finished product after freeze-dried.
To achieve these goals, the technical solution adopted by the present invention is as follows：Polyphenol oxidase carries in a kind of lychee exocarp
Method is taken, is comprised the following steps：
（1）Fresh lychee exocarp is put into after being dried in drying baker, taking-up is placed on the place of drying and naturally cools to room temperature, places into and beats
Mechanical crushing was the powder of 30 mesh standard sieves in powder machine, wherein, the temperature of the drying baker is 80-100 DEG C, and drying time is
8-12h；
（2）In step（1）Aqueous acetone solution is added in resulting powder, and after 15-20h is stood under the conditions of 15 DEG C of ﹣ 10- ﹣
Supernatant is taken, it is standby to obtain thick zyme extract, wherein, the mass fraction of the aqueous acetone solution is 70-80%；The acetone of addition
Aqueous solution volume is 6-8 times of powder；
（3）To step（2）Ethanol solution is added in the thick zyme extract of gained, and in 125-250W, ultrasound under the conditions of 60-80 DEG C
Ripple is extracted 2-3 times, and 1-2h is extracted every time, then filters merging filtrate, wherein, the mass fraction of the ethanol solution is 75-
80%；The volume ratio 1 of thick zyme extract and ethanol solution：1-1：2；
（4）By the filtrate after merging through decompression, ethanol is reclaimed, then concentrating filter liquor, freeze-drying are obtained into required polyphenol oxygen
Change enzyme.
In sum, by adopting the above-described technical solution, the beneficial effects of the invention are as follows：
（1）Extracting method of the present invention is used after the drying of fresh lichee shell is clayed into power, and first passes through the extraction of crude enzyme liquid, then through super
Sound wave is extracted, and can efficiently be extracted the polyphenol oxidase in lychee exocarp, improves recovery rate；
（2）Extracting method of the present invention is simple, and recovery rate is high, and gained polyphenol oxidase can be widely used for medicine, food and related neck
Domain.
Specific embodiment
Below by way of specific embodiment, the invention will be further described.
The extracting method of polyphenol oxidase in a kind of lychee exocarp of embodiment 1, is realized using following steps：
（1）Fresh lychee exocarp is put into after drying 8h in the drying baker that temperature is 80 DEG C, taking-up is placed on natural cooling at dry
To room temperature, the powder that mechanical crushing in powder beater was 30 mesh standard sieves is placed into；
（2）In step（1）Add the aqueous acetone solution that 6 times of powder volume and mass fraction are 70% in resulting powder, and
Supernatant is taken after standing 15h under the conditions of 10 DEG C of ﹣, thick zyme extract is obtained standby；
（3）To step（2）It is 1 to be added in the thick zyme extract of gained with its volume ratio：1 and mass fraction be 75% ethanol solution,
And in 125W, 1h is extracted in ultrasonic wave extraction 2 times under the conditions of 60 DEG C every time, then filters merging filtrate；
（4）By the filtrate after merging through decompression, ethanol is reclaimed, then concentrating filter liquor, freeze-drying are obtained into required polyphenol oxygen
Change enzyme.
The extracting method of polyphenol oxidase in a kind of lychee exocarp of embodiment 2, is realized using following steps：
（1）Fresh lychee exocarp is put into after drying 12h in the drying baker that temperature is 100 DEG C, taking-up is placed on naturally cold at dry
But to room temperature, the powder that mechanical crushing in powder beater was 30 mesh standard sieves is placed into；
（2）In step（1）Add the aqueous acetone solution that 8 times of powder volume and mass fraction are 80% in resulting powder, and
Supernatant is taken after standing 20h under the conditions of 15 DEG C of ﹣, thick zyme extract is obtained standby；
（3）To step（2）It is 2 to be added in the thick zyme extract of gained with its volume ratio:1 and mass fraction be 80% ethanol solution,
And in 250W, 2h is extracted in ultrasonic wave extraction 3 times under the conditions of 80 DEG C every time, then filters merging filtrate；
（4）By the filtrate after merging through decompression, ethanol is reclaimed, then concentrating filter liquor, freeze-drying are obtained into required polyphenol oxygen
Change enzyme.
Claims (6)
1. in a kind of lychee exocarp polyphenol oxidase extracting method, it is characterised in that：Comprise the following steps：
（1）Fresh lychee exocarp is put into after being dried in drying baker, taking-up is placed on the place of drying and naturally cools to room temperature, places into and beats
Mechanical crushing was the powder of 30 mesh standard sieves in powder machine；
（2）In step（1）Aqueous acetone solution is added in resulting powder, and after 15-20h is stood under the conditions of 15 DEG C of 10 ~ ﹣ of ﹣
Supernatant is taken, thick zyme extract is obtained standby；
（3）To step（2）Ethanol solution is added in the thick zyme extract of gained, and in 125-250W, ultrasound under the conditions of 60-80 DEG C
Ripple is extracted 2-3 times, and 1-2h is extracted every time, then filters merging filtrate；
（4）By the filtrate after merging through decompression, ethanol is reclaimed, then concentrating filter liquor, freeze-drying are obtained into required polyphenol oxygen
Change enzyme.
2. in a kind of lychee exocarp according to claim 1 polyphenol oxidase extracting method, it is characterised in that：The step
（1）The temperature of middle drying baker is 80-100 DEG C, and drying time is 8-12h.
3. in a kind of lychee exocarp according to claim 1 polyphenol oxidase extracting method, it is characterised in that：The step
（2）The mass fraction of middle aqueous acetone solution is 70-80%.
4. in a kind of lychee exocarp according to claim 1 polyphenol oxidase extracting method, it is characterised in that：The step
（2）The aqueous acetone solution volume of middle addition is 6-8 times of powder.
5. in a kind of lychee exocarp according to claim 1 polyphenol oxidase extracting method, it is characterised in that：The step
（3）The mass fraction of middle ethanol solution is 75-80%.
6. in a kind of lychee exocarp according to claim 1 polyphenol oxidase extracting method, it is characterised in that：The step
（3）In thick zyme extract and ethanol solution volume ratio 1：1-1：2.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611144724.9A CN106754771A (en) | 2016-12-13 | 2016-12-13 | The extracting method of polyphenol oxidase in a kind of lychee exocarp |
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| CN201611144724.9A CN106754771A (en) | 2016-12-13 | 2016-12-13 | The extracting method of polyphenol oxidase in a kind of lychee exocarp |
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| CN201611144724.9A Pending CN106754771A (en) | 2016-12-13 | 2016-12-13 | The extracting method of polyphenol oxidase in a kind of lychee exocarp |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114504615A (en) * | 2022-02-22 | 2022-05-17 | 青岛科技大学 | Extract composition with liver protecting and enzyme reducing effects |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102477413A (en) * | 2010-11-25 | 2012-05-30 | 张晓雷 | Separation technology for polyphenol oxidase of tobacco |
-
2016
- 2016-12-13 CN CN201611144724.9A patent/CN106754771A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102477413A (en) * | 2010-11-25 | 2012-05-30 | 张晓雷 | Separation technology for polyphenol oxidase of tobacco |
Non-Patent Citations (1)
| Title |
|---|
| 熊何健等: "荔枝壳中多酚氧化酶活性研究", 《食品科学》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114504615A (en) * | 2022-02-22 | 2022-05-17 | 青岛科技大学 | Extract composition with liver protecting and enzyme reducing effects |
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Application publication date: 20170531 |