CN106754545A - A kind of indoor method of the high-purity culture with spirulina is taken food - Google Patents
A kind of indoor method of the high-purity culture with spirulina is taken food Download PDFInfo
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- CN106754545A CN106754545A CN201710031090.4A CN201710031090A CN106754545A CN 106754545 A CN106754545 A CN 106754545A CN 201710031090 A CN201710031090 A CN 201710031090A CN 106754545 A CN106754545 A CN 106754545A
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- spirulina
- culture
- algae
- nutrient solution
- culture vessel
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/02—Separating microorganisms from their culture media
Abstract
The invention provides a kind of indoor method of the high-purity culture with spirulina is taken food, following steps are specifically included：(1) sterilized using boiling water；(2) nutrient source A, B, C, D, E and F are sequentially added into successively and nutrient solution is prepared in pure water, and add algae kind culture；(3) cultivate：In 26 34 DEG C, daily illumination 18 20 hours；(4) algae solution is extracted using miniature diaphragm self priming pump, deadweight collects filtering gained spirulina through strainer filtering, and filter screen is using preceding through washing, high temperature or ultraviolet disinfection；(5) pure water is sprayed with pre- adherence along the wall of culture vessel four；Liquid inoculation is changed after (6) one months and enters new cultivation cycle.The present invention does not use the chemical fertilizer such as carbon ammonia, urea only with food grade inorganic salt and drinking water configuration nutrient solution, agricultural chemicals and hormone regulator is not used, it is ensured that spirulina has higher quality；Using the purifying culture spirulina of closed, it is to avoid living contaminants；Take algae convenient, can reach and taken food in growth.
Description
Technical field
It is the invention belongs to both culturing microalgae technical field, and in particular to a kind of cultural method of spirulina more particularly to a kind of
Indoor method of the high-purity culture with spirulina is taken food.
Background technology
Spirulina has been widely used in daily life as health food, medicine or food additives etc..And, spiral
The ecosystem environment of algae is the carbonate lakes and marhshes or wetland of high temperature, intense light irradiation and high concentration, therefore, it is high-quality, optimum from
Right condition limits industrialized production.At present, the production of spirulina industrialization all over the world is by the outdoor of extensive style
Great Chi, vinyl house combination great Chi, large-scale photosynthesis physiological target industrialized production etc..Again by manually borrowing after culture
Help filter screen filtration, rinse, and machine sterilizing, high temperature drying, shaping and pack etc. process industrial food is processed into eat,
There is heavy metal and contaminant problem is easily sent out, bioactivity is not high, nutrition leak is serious, while the also color containing industry processes
Element, essence, additive and preservative etc..In addition, the cell membrane Main Ingredients and Appearance of algae is peptide glycan, therefore filament is very fragile,
Screen pack, the water pressure of shower, sterilizing that misoperation during incubation and post-production is such as cultivated circulation, takes algae
Mode, dry temperature excessively it is high can cause its be broken or cytoclasis, influence quality, take algae amount, or even its overflow battalion
Foster thing can bring the growth of microorganism, influence the lasting culture of spirulina.
A kind of purifying SPIRULINA CULTIVATION method of closed and the convenient and swift algae method that takes are developed for this turns into urgently to be resolved hurrily
Technical problem.
The content of the invention
The invention provides a kind of indoor method of the high-purity culture with spirulina is taken food, nutrient solution is only containing inorganic salts and pure
Water purification, and use the purifying culture of closed, it is ensured that spirulina has higher quality and purity, and it is convenient to take algae, can reach side life
Side long takes algae.
The technical proposal for solving the technical problem of the invention is：
A kind of indoor method of the high-purity culture with spirulina is taken food, specifically includes following steps：
(1) sterilization of culture vessel：To running water is added in culture vessel, 100 DEG C are heated to, are poured out after boiling 5-15 minutes
Running water.Next cultivation cycle is prepared to enter into after either new engine comes into operation, or a upper cultivation cycle terminates, all
Machine is thoroughly cleaned with running water, the sterilization of culture vessel is then carried out again.If not carrying out thorough disinfection, it is likely that
Miscellaneous bacteria, the pollution of miscellaneous algae can be brought so that culture cannot continue or quality is very poor.Therefore, applicants studied ultraviolet disinfection, wine
Smart sterilization, ozonization, hydrogen peroxide, potassium permanganate or several ways such as are combined at various disinfection way, but effect is all paid no attention to
Think.Ultraviolet light cannot be sterilized to the place of the corner crack of opaque article, and scarce capacity is killed to miscellaneous algae；Alcohol fails effectively
Miscellaneous algae is killed, and it is cumbersome；Ozone manipulation is difficult, disinfecting time is very long, there is disclosure risk；Hydrogen peroxide has the carcinogenic wind of residual
Danger；Potassium permanganate has color to be difficult to remove.The present invention in culture vessel using directly heating a certain amount of water to seething with excitement and produce
Producing water vapor, is measured after seething with excitement by temperature detector, controls fluidized state certain time to sterilize by central control system,
So that thorough disinfection, sterilizing can be obtained inside culture vessel with each gap of upper lid, corner.For being hidden in upper lid cavity
Take phytem system pipeline and water pump, cannot only be sterilized by boiling water and steam, sterilization method be temperature detector measure boiling water it
Afterwards, water pump extracting high-temperature vapor is started by central control system and certain time is come thorough disinfection.
(2) it is inoculated with liquid：Pure water is added in culture vessel, nutrient source A is first added, added after stirring, fully dissolving
B, in this way, order input nutrient source A, B, C, D, E and F obtain nutrient solution according to this, then heat the temperature of nutrient solution
Or it is cooled to 26-34 degree.
Appropriate spirulina algae kind is finally added to be cultivated.The basis of appropriate algae kind inoculum concentration：By optical density
Postvaccinal algae solution concentration i.e. OD=0.3-0.5 is measured when tester is with wavelength 560nm to be advisable.Then according to the appearance of culture vessel
Product size, draws corresponding suitable inoculum concentration.
Directly match somebody with somebody liquid in container, coordinate whole system to closed requirement, reduce some unnecessary links, reduce
Nutrient solution is by utensil, the risk of air pollution.Nutrient solution is prepared only with inorganic salts and pure water, and carbon ammonia, urea etc. are not used
Chemical fertilizer, does not use agricultural chemicals, does not use hormone regulator, it is ensured that gained spirulina has higher quality.
The A is NaHCO3、CH3COONa、NaNO3And NaCl, B are K2HPO4·3H2O、K2SO4And MgSO4·7H2O, C
It is EDTANa2And FeSO4·7H2O, D are CaCl2·2H2O, E are H3BO3、MnCl2·4H2O、ZnSO4·7H2O、MoO3With
CuSO4·5H2O, F are NH4VO3、NiSO4·7H2O、NaWO4、Ti(SO4)2And Co（NO3）2·6H2O；
(3) cultivate：By central control system and programme-control, culture-liquid temp remains to 26-34 DEG C, and 30000- is used daily
50000Lux's is red, white, blue respectively by the LED light source of 60~75%, 15~25%, 10~15% combinations, especially with optimal proportion 7：
2：1 combination illumination 18-20 hours, while the circulatory system of drive control nutrient solution, keeps algae solution with 15-20 cels
Speed convection circulation, the growth efficiency and quality highest of such spirulina.The growth that spirulina will keep good in algae solution is needed
Uniform illumination and uniform nutrient solution are wanted, therefore is to need algae solution convection circulation.The convection circulation speed of 15-20 centimeters per seconds
Degree, most beneficial for the growth of spirulina.
(4) algae is taken：After inoculation 48 hours, when nutrient solution OD values reach 1-1.2, extracted using miniature diaphragm self priming pump
Algae solution, is evacuated to the liquid level 6-12 centimeters of culture vessel, is filtered through filter bowl by conducting oneself with dignity, and crosses net nutrient solution and immature algal filament is returned
It flow to culture vessel to continue to cultivate, ripe spirulina is stayed in filter bowl, i.e. direct-edible after being rinsed through water.Algae 3- is can use daily
9 times, take every time at most desirable 3 parts points for 3 times, take points for 9 times and at most to take 1 part every time.
The filter bowl, through washing, ultraviolet or high-temperature sterilization 5-15 minutes, prevents bacterium or miscellaneous algae pollution before algae is taken every time.
Algae solution is extracted by the miniature diaphragm self priming pump chosen and does not hinder filament.By central control system control self priming pump
Every part of extraction time effectively controls to take algae amount.Daily algae amount is taken not above average daily accumulation biomass.
(5) adherent prevention and moisturizing：Work 2-5 times daily, the pure water of each 30-150ml is along the wall of culture vessel four
Spray pure water.Timing spray the wall of culture vessel four, can moisturizing prevent spirulina adherent again.Culture vessel inwall, just there is few
Amount spirulina is attached, and when not yet growth, nutrient solution is entered under the water shower sprayed by shower nozzle, so can effectively be prevented
Spirulina growth at this point and the corruption after growing, prevent the negative effect to edible safety and consumption experience.
(6) liquid is changed：After one month, nutrient source is prepared again, and inoculation enters next cultivation cycle again.
Preferably, the addition concentration of nutrient source is respectively described in step (2)：NaHCO37.2g/L, CH3COONa 4.8
G/L, NaNO3 2.5g/L, NaCl1.5g/L, K2HPO4·3H2O 0.6 g/L, K2SO41.0g/L, MgSO4·7H2O 0.4g/
L, FeSO4·7H2O 0.008g/L, EDTANa20.06g/L, CaCl2·2H2O 0.06g/L, H3BO3 3×10-3G/L,
MnCl2·4H2O 2×10-3G/L, ZnSO4·7H2O 0.2×10-3G/L, MoO3 0.01×10-3G/L, CuSO4·5H2O
0.08×10-3G/L, NH4VO3 23×10-6G/L, NiSO4·7H2O 48×10-6G/L, NaWO4 18×10-6G/L, Ti
(SO4)2 40×10-6G/L, Co (NO3)2·6H2O 5×10-6g/L。
Preferably, convection circulation described in step (3) is using propeller type blades stirring and culture vessel inwall water conservancy diversion
The mode that plate stirring is combined is completed.Regular circulation mode uses air pump compresses gas, the circulation of algae solution is driven with air-blowing, easily
The miscellaneous algae pollution nutrient solution of miscellaneous bacteria for producing outside air to bring；What common blade stirrer was produced be water body eddy circulating rather than
Convection circulation, and stirring dynamics is inadequate；Agitating mode of the present invention, can make the effective convection circulation of algae solution so that illumination
Evenly, the photoperiod is more reasonable.
Beneficial effects of the present invention are：
1st, only with food grade inorganic salt and drinking water configuration nutrient solution, the chemical fertilizer such as carbon ammonia, urea are not used, do not use agriculture
Medicine, does not use hormone regulator, it is ensured that spirulina has higher quality；
2nd, using the purifying culture spirulina of closed, oxygen and air exchange will prevent dust, micro- by bacterium level filter membrane
Biological, miscellaneous algae etc. pollutes, it is ensured that high-quality；
3rd, the natural ecological environment of spirulina is simulated by intelligent home appliance, it is not necessary to sunlight, not by region, season, gas
The extraneous factors such as time, weather are limited；
4th, it is not processed, existing enchashment food, fresh food, it is ensured that it is natural it is primary without being lost in, it is no added；
5th, can reach and taken food in growth, daily uninterrupted, the advantage in this unique and forward position will bring a kind of complete to the mankind
New life style.
Specific embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to embodiments to the present invention
It is further elaborated.It should be appreciated that specific embodiment described herein is only used to explain the present invention, limit is not used to
The fixed present invention.
Embodiment 1
(1) sterilize：To the running water that 10L is added in 50L culture vessels, 100 DEG C are heated to, are poured out after boiling 5-15 minutes certainly
Water；
(2) it is inoculated with liquid：50L pure water is added in culture vessel, 800g nutrient source A are first added, added after stirring and dissolving
100gB, in this way, order input nutrient source A, B, C, D, E and F obtain nutrient solution according to this, add the spiral of 25-30g
Algae algae kind is cultivated；
(3) cultivate：It is 26-34 DEG C to keep culture-liquid temp, presses 7 with the red, white, blue of 30000-50000Lux daily：2：1 group
The LED light source illumination of conjunction 18-20 hours, while convection circulation speed loop of the nutrient solution using 15-20 cels；
(4) algae is taken：After 48 hours, when nutrient solution OD values reach 1-1.2,3-5L algae solutions are extracted using miniature diaphragm self priming pump,
The liquid level 6-12 centimeters of culture vessel are evacuated to, deadweight collects filtering gained through strainer filtering, the backflow of gained nutrient solution
The spirulina of 9-18g；
(5) adherent prevention and moisturizing：At regular time and quantity pure water is sprayed along the wall of culture vessel four；
(6) liquid is changed：After one month, nutrient source is prepared again, and inoculation enters next cultivation cycle again.
Presently preferred embodiments of the present invention is the foregoing is only, is not intended to limit the invention, it is all in essence of the invention
Any modification, equivalent and improvement made within god and principle etc., should be included within the scope of the present invention.
Claims (3)
1. a kind of indoor method of the high-purity culture with spirulina is taken food, it is characterised in that specifically include following steps：
(1) sterilize：To running water is added in culture vessel, 100 DEG C are heated to, running water is poured out after boiling 5-15 minutes；
(2) it is inoculated with liquid：Pure water is added in culture vessel, nutrient source A is first added, B is added after stirring, fully dissolving, pressed
Method, sequentially puts into nutrient source A, B, C, D, E and F and obtains nutrient solution according to this like this, adds spirulina algae kind and is cultivated, so
The temperature of nutrient solution is heated or is cooled to 26-34 degree afterwards；
The A is NaHCO3、CH3COONa、NaNO3And NaCl, B are K2HPO4·3H2O、K2SO4And MgSO4·7H2O, C are
EDTANa2And FeSO4·7H2O, D are CaCl2·2H2O, E are H3BO3、MnCl2·4H2O、ZnSO4·7H2O、MoO3With
CuSO4·5H2O, F are NH4VO3、NiSO4·7H2O、NaWO4、Ti(SO4)2And Co（NO3）2·6H2O；
(3) cultivate：By central control system and programme-control, culture-liquid temp remains to 26-34 DEG C, and 30000- is used daily
60~75%, 15~25%, 10~15% LED light source illumination combined 18-20 hours is pressed in the red, white, blue of 50000Lux respectively, together
When drive control nutrient solution the circulatory system, keep algae solution with the speed convection circulation of 15-20 cels；
(4) algae is taken：After inoculation 48 hours, when nutrient solution OD values reach 1-1.2, algae solution is extracted using miniature diaphragm self priming pump,
The liquid level 6-12 centimeters of culture vessel are evacuated to, are filtered through filter bowl by conducting oneself with dignity, crossed net nutrient solution and immature algal filament is back to
Culture vessel continues to cultivate, and ripe spirulina is stayed in filter bowl, i.e. direct-edible after being rinsed through water；The filter bowl is taking every time
Through washing, ultraviolet or high-temperature sterilization 5-15 minutes before algae；
(5) adherent prevention and moisturizing：Work 2-5 times daily, the sterile purified water of each 30-150ml is along the wall of culture vessel four
Spray；
(6) liquid is changed：After one month, nutrient source is prepared again, and inoculation enters next cultivation cycle again.
2. method of the interior high-purity culture with spirulina is taken food as claimed in claim 1, it is characterised in that in step (2)
The addition concentration of the nutrient source is respectively：NaHCO37.2g/L, CH3COONa 4.8 g/L, NaNO3 2.5g/L,
NaCl1.5g/L, K2HPO4·3H2O 0.6 g/L, K2SO41.0g/L, MgSO4·7H2O 0.4g/L, FeSO4·7H2O
0.008g/L, EDTANa20.06g/L, CaCl2·2H2O 0.06g/L, H3BO3 3×10-3G/L, MnCl2·4H2O 2×10- 3G/L, ZnSO4·7H2O 0.2×10-3G/L, MoO3 0.01×10-3G/L, CuSO4·5H2O 0.08×10-3G/L, NH4VO3
23×10-6G/L, NiSO4·7H2O 48×10-6G/L, NaWO4 18×10-6G/L, Ti (SO4)2 40×10-6G/L, Co
(NO3)2·6H2O 5×10-6g/L。
3. method of the interior high-purity culture with spirulina is taken food as claimed in claim 1, it is characterised in that in step (3)
The convection circulation is stirred using propeller type blades and completed by the way of the stirring of culture vessel inwall deflector is combined.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109234209A (en) * | 2018-11-16 | 2019-01-18 | 杭州源生泰生物科技股份有限公司 | A kind of method of domestic edible spirulina |
| US11134688B2 (en) * | 2018-09-13 | 2021-10-05 | Heliae Development, Llc | Compositions and methods for indirectly reducing incidence of fungal pathogen activity in plants |
| CN114947136A (en) * | 2022-03-22 | 2022-08-30 | 湖北康健医药科技有限公司 | A method for producing Spirulina directly used for preparing fresh food |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1254012A (en) * | 1998-11-16 | 2000-05-24 | 思可达高技术产业化中试配套有限公司 | Method for house cultivating edible fresh spirulina |
| CN101575567A (en) * | 2009-06-22 | 2009-11-11 | 北京科技大学 | Method for culturing microalgae by illumination way and reactor thereof |
| CN101705189A (en) * | 2009-10-28 | 2010-05-12 | 江西新大泽实业集团有限公司 | Spirulina mixed culturing technology and device |
| CN105838612A (en) * | 2016-04-26 | 2016-08-10 | 南昌大学 | Method for treating Spirulina culture wastewater by using multistage membrane separation technique |
-
2017
- 2017-01-17 CN CN201710031090.4A patent/CN106754545A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1254012A (en) * | 1998-11-16 | 2000-05-24 | 思可达高技术产业化中试配套有限公司 | Method for house cultivating edible fresh spirulina |
| CN101575567A (en) * | 2009-06-22 | 2009-11-11 | 北京科技大学 | Method for culturing microalgae by illumination way and reactor thereof |
| CN101705189A (en) * | 2009-10-28 | 2010-05-12 | 江西新大泽实业集团有限公司 | Spirulina mixed culturing technology and device |
| CN105838612A (en) * | 2016-04-26 | 2016-08-10 | 南昌大学 | Method for treating Spirulina culture wastewater by using multistage membrane separation technique |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11134688B2 (en) * | 2018-09-13 | 2021-10-05 | Heliae Development, Llc | Compositions and methods for indirectly reducing incidence of fungal pathogen activity in plants |
| CN109234209A (en) * | 2018-11-16 | 2019-01-18 | 杭州源生泰生物科技股份有限公司 | A kind of method of domestic edible spirulina |
| CN114947136A (en) * | 2022-03-22 | 2022-08-30 | 湖北康健医药科技有限公司 | A method for producing Spirulina directly used for preparing fresh food |
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Application publication date: 20170531 |