CN106749745A - Sugared polysaccharide of thorn and preparation method thereof - Google Patents
Sugared polysaccharide of thorn and preparation method thereof Download PDFInfo
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- CN106749745A CN106749745A CN201710105241.6A CN201710105241A CN106749745A CN 106749745 A CN106749745 A CN 106749745A CN 201710105241 A CN201710105241 A CN 201710105241A CN 106749745 A CN106749745 A CN 106749745A
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- thorn
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- icing sugar
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
It is sugared polysaccharide of a kind of thorn and preparation method thereof the present invention relates to pierce sugared extractive technique field, thorn sugar polysaccharide is obtained in the steps below：The first step, will be obtained piercing Icing Sugar after piercing sugared medicinal material drying and crushing, then will pierce Icing Sugar and petroleum ether backflow degreasing, and Aqueous extracts are obtained after the thorn Icing Sugar after degreasing and distilled water are carried out into refluxing extraction.The preparation method of the sugared polysaccharide of thorn of the present invention, step is less, and method is simple, it is easy to operate, and according to preparation method of the present invention, to the extraction rate reached of the sugared polysaccharide of thorn in thorn sugar to 8.65% to 8.79%；Furthermore, the purity of the sugared polysaccharide of thorn obtained according to the present invention is high, quality better, with obvious immunoregulatory activity, can strengthen immune effect, has broad application prospects and good economic worth.
Description
Technical field
It is sugared polysaccharide of a kind of thorn and preparation method thereof the present invention relates to pierce sugared extractive technique field.
Background technology
The particle that thorn sugar condenses for the liquid glucose of legume camel thorn branches and leaves secretion, color is yellowish-white, and taste is sweet, somewhat glues
Property, it is Uygur medicine medicinal herbs most in use, the diseases such as dysentery, diarrhoea, blood urine can be treated.The Uygur nationality is among the people often by it together with milk
Take, claim its property heat and it is dry, can begin sensible, strengthening body.Modern study shows that the main component of thorn sugar is polysaccharide.Mesh
Before, the separation of polysaccharides purification technique in thorn sugar is also had been reported that, but the step of its separating-purifying is complicated, is not easy to market and pushes away
Extensively.
The content of the invention
The invention provides sugared polysaccharide of one kind thorn and preparation method thereof, the deficiency of above-mentioned prior art is overcome, it can have
It is complicated that effect solves the problems, such as that the sugared separation of polysaccharides technology of existing thorn has separating-purifying step.
One of technical scheme is realized by following measures：The sugared polysaccharide of one kind thorn, in the steps below
Arrive：The first step, will obtain piercing Icing Sugar after piercing sugared medicinal material drying and crushing, then will pierce Icing Sugar and petroleum ether backflow degreasing, will be de-
Thorn Icing Sugar after fat obtains Aqueous extracts after carrying out refluxing extraction with distilled water, wherein, thorn Icing Sugar is 1 with the volume ratio of distilled water:7
To 9；Second step, obtains piercing sugared concentrate after 1/to five/6th that Aqueous extracts are concentrated under reduced pressure into Aqueous extracts volume；
3rd step, to the ethanol water that 95% is added in the sugared concentrate of thorn until the alcohol precipitation concentration in the sugared concentrate of thorn is 80% to 90%,
The thorn sugar concentrate of 95% ethanol water will be added to obtain standing liquid after standing 20h to 24h；4th step, will stand liquid is carried out
Filter cake is obtained after suction filtration, filter cake sequentially is obtained piercing sugared polysaccharide by after absolute ethanol washing, acetone washing, vacuum drying.
Here is the further optimization and/or improvements to one of foregoing invention technical scheme：
In the above-mentioned first step, the time of refluxing extraction is 1h to 2h.
In the above-mentioned first step, the temperature of refluxing extraction is 75 DEG C to 90 DEG C.
In above-mentioned 3rd step, dwell temperature is 5 DEG C to 8 DEG C.
The two of technical scheme are realized by following measures：A kind of described thorn sugar of one of technical scheme
The preparation method of polysaccharide, is carried out in the steps below：The first step, obtains piercing Icing Sugar by the sugared medicinal material drying of thorn and after crushing, and then will
Thorn Icing Sugar and petroleum ether backflow degreasing, Aqueous extracts are obtained after the thorn Icing Sugar after degreasing and distilled water are carried out into refluxing extraction, wherein,
Thorn Icing Sugar is 1 with the volume ratio of distilled water:7 to 9；Aqueous extracts are concentrated under reduced pressure into 1/6th of Aqueous extracts volume by second step
Obtain piercing sugared concentrate after to 1/5th；3rd step, to being added in the sugared concentrate of thorn, 95% ethanol water is dense up to piercing sugar
Alcohol precipitation concentration in contracting liquid is 80% to 90%, and the thorn sugar concentrate of 95% ethanol water will be added to be obtained after standing 20h to 24h
To standing liquid；4th step, will be stood after liquid carries out suction filtration and obtains filter cake, and filter cake is sequentially washed by absolute ethanol washing, acetone
Wash, be vacuum dried after obtain piercing sugared polysaccharide.
Here is the further optimization and/or improvements to the two of foregoing invention technical scheme：
In the above-mentioned first step, the time of refluxing extraction is 1h to 2h.
In the above-mentioned first step, the temperature of refluxing extraction is 75 DEG C to 90 DEG C.
In above-mentioned 3rd step, dwell temperature is 5 DEG C to 8 DEG C.
The preparation method of the sugared polysaccharide of thorn of the present invention, step is less, and method is simple, it is easy to operate, and according to this
Described preparation method is invented, the extraction rate reached for piercing sugar polysaccharide to thorn in sugared to 8.65% to 8.79%；Furthermore, according to the present invention
The purity of the sugared polysaccharide of thorn for obtaining is high, quality better, with obvious immunoregulatory activity, can strengthen immune effect, with wide
Wealthy application prospect and good economic worth.
Specific embodiment
The present invention is not limited by following embodiments, can technology according to the present invention scheme and actual conditions determine specifically
Implementation method.Various chemical reagent and chemical article are previously mentioned in the present invention unless otherwise specified, are public in the prior art
Know public chemical reagent and chemical article；Percentage in the present invention is as without specified otherwise, being mass percent.
With reference to embodiment, the invention will be further described：
Embodiment 1：Thorn sugar polysaccharide, is obtained by following preparation methods：The first step, is pierced by the sugared medicinal material drying of thorn and after crushing
Icing Sugar, then will pierce Icing Sugar and petroleum ether backflow degreasing, be obtained after the thorn Icing Sugar after degreasing is carried out into refluxing extraction with distilled water
Aqueous extracts, wherein, thorn Icing Sugar is 1 with the volume ratio of distilled water:7 to 9；Aqueous extracts are concentrated under reduced pressure into water extraction liquid by second step
Obtain piercing sugared concentrate after long-pending 1/1st to five/6th；3rd step, the ethanol to addition 95% in the sugared concentrate of thorn is water-soluble
Liquid is 80% to 90% up to the alcohol precipitation concentration in the sugared concentrate of thorn, and the sugared concentrate of thorn of 95% ethanol water will be added to stand
Obtain standing liquid after 20h to 24h；4th step, will stand after liquid carries out suction filtration and obtains filter cake, by filter cake sequentially by absolute ethyl alcohol
Obtain piercing sugared polysaccharide after washing, acetone washing, vacuum drying.
By the present embodiment as can be seen that the preparation method of the sugared polysaccharide of thorn described in the present embodiment, step is less, method letter
List, it is easy to operate, and the preparation method according to the present embodiment, the extraction rate reached to the sugared polysaccharide of thorn in thorn sugar are arrived
8.65% to 8.79%；Furthermore, the purity of the sugared polysaccharide of thorn obtained according to the present embodiment is high, quality better, immune is adjusted with obvious
Section activity, can strengthen immune effect, have broad application prospects and good economic worth.
Embodiment 2：Thorn sugar polysaccharide, is obtained by following preparation methods：The first step, obtains by the sugared medicinal material drying of thorn and after crushing
To thorn Icing Sugar, Icing Sugar and petroleum ether backflow degreasing then will be pierced, after the thorn Icing Sugar after degreasing and distilled water are carried out into refluxing extraction
Aqueous extracts are obtained, wherein, thorn Icing Sugar is 1 with the volume ratio of distilled water:7 or 9；Aqueous extracts are concentrated under reduced pressure into water extraction by second step
Obtain piercing sugared concentrate after 1/6th or 1/5th of liquid product；3rd step, to the ethanol that 95% is added in the sugared concentrate of thorn
The aqueous solution is 80% or 90% up to the alcohol precipitation concentration in the sugared concentrate of thorn, will add the sugared concentrate of thorn of 95% ethanol water
Obtain standing liquid after standing 20h or 24h；4th step, will stand after liquid carries out suction filtration and obtains filter cake, by filter cake sequentially by anhydrous
Obtain piercing sugared polysaccharide after ethanol washing, acetone washing, vacuum drying.
Embodiment 3：Used as the optimization of above-described embodiment, in the first step, the time of refluxing extraction is 1h to 2h.
Embodiment 4：Used as the optimization of above-described embodiment, in the first step, the temperature of refluxing extraction is 75 DEG C to 90 DEG C.
Embodiment 5：Used as the optimization of above-described embodiment, in the 3rd step, dwell temperature is 5 DEG C to 8 DEG C.
Embodiment 6：Thorn sugar polysaccharide, is obtained by following preparation methods：The first step, obtains by the sugared medicinal material drying of thorn and after crushing
To thorn Icing Sugar, Icing Sugar and petroleum ether backflow degreasing then will be pierced, after the thorn Icing Sugar after degreasing and distilled water are carried out into refluxing extraction
Aqueous extracts are obtained, wherein, thorn Icing Sugar is 1 with the volume ratio of distilled water:7, the time of refluxing extraction is 2h, the temperature of refluxing extraction
It is 75 DEG C；Second step, obtains piercing sugared concentrate after 1/6th that Aqueous extracts are concentrated under reduced pressure into Aqueous extracts volume；3rd step,
To the ethanol water that 95% is added in the sugared concentrate of thorn until the alcohol precipitation concentration in the sugared concentrate of thorn is 80%, 95% will be added
The thorn sugar concentrate of ethanol water obtains standing liquid after standing 24h, and dwell temperature is 5 DEG C；4th step, will stand liquid and is taken out
Filter cake is obtained after filter, filter cake sequentially is obtained piercing sugared polysaccharide by after absolute ethanol washing, acetone washing, vacuum drying.According to
Preparation method described in the present embodiment, to the extraction rate reached of the sugared polysaccharide of thorn in thorn sugar to 8.65%.
Embodiment 7：Thorn sugar polysaccharide, is obtained by following preparation methods：The first step, obtains by the sugared medicinal material drying of thorn and after crushing
To thorn Icing Sugar, Icing Sugar and petroleum ether backflow degreasing then will be pierced, after the thorn Icing Sugar after degreasing and distilled water are carried out into refluxing extraction
Aqueous extracts are obtained, wherein, thorn Icing Sugar is 1 with the volume ratio of distilled water:9, the time of refluxing extraction is 1h, the temperature of refluxing extraction
It is 90 DEG C；Second step, obtains piercing sugared concentrate after 1/5th that Aqueous extracts are concentrated under reduced pressure into Aqueous extracts volume；3rd step,
To the ethanol water that 95% is added in the sugared concentrate of thorn until the alcohol precipitation concentration in the sugared concentrate of thorn is 90%, 95% will be added
The thorn sugar concentrate of ethanol water obtains standing liquid after standing 20h, and dwell temperature is 8 DEG C；4th step, will stand liquid and is taken out
Filter cake is obtained after filter, filter cake sequentially is obtained piercing sugared polysaccharide by after absolute ethanol washing, acetone washing, vacuum drying.According to
Preparation method described in the present embodiment, to the extraction rate reached of the sugared polysaccharide of thorn in thorn sugar to 8.79%
In sum, the preparation method of the sugared polysaccharide of thorn of the present invention, step is less, and method is simple, it is easy to operate, and root
According to preparation method of the present invention, to the extraction rate reached of the sugared polysaccharide of thorn in thorn sugar to 8.65% to 8.79%；Furthermore, according to this
The purity of the sugared polysaccharide of thorn that invention is obtained is high, quality better, with obvious immunoregulatory activity, can strengthen immune effect, has
Have broad application prospects and good economic worth.
Above technical characteristic constitutes embodiments of the invention, and it has stronger adaptability and implementation result, can basis
The non-essential technical characteristic of increase and decrease is actually needed to meet the demand of different situations.
Claims (10)
1. it is a kind of to pierce sugared polysaccharide, it is characterised in that to obtain in the steps below：The first step, obtains by the sugared medicinal material drying of thorn and after crushing
Thorn Icing Sugar, then by thorn Icing Sugar and petroleum ether backflow degreasing, obtains after the thorn Icing Sugar after degreasing and distilled water are carried out into refluxing extraction
To Aqueous extracts, wherein, thorn Icing Sugar is 1 with the volume ratio of distilled water:7 to 9；Aqueous extracts are concentrated under reduced pressure into Aqueous extracts by second step
Obtain piercing sugared concentrate after 1st/to five/6th of volume；3rd step, to the ethanol water that 95% is added in the sugared concentrate of thorn
Solution is 80% to 90% up to the alcohol precipitation concentration in the sugared concentrate of thorn, and the sugared concentrate of thorn of 95% ethanol water will be added quiet
Obtain standing liquid after putting 20h to 24h；4th step, will stand after liquid carries out suction filtration and obtains filter cake, by filter cake sequentially by anhydrous second
Obtain piercing sugared polysaccharide after alcohol washing, acetone washing, vacuum drying.
2. according to claim 1 to pierce sugared polysaccharide, it is characterised in that in the first step, the time of refluxing extraction is 1h to 2h.
3. according to claim 1 and 2 to pierce sugared polysaccharide, it is characterised in that in the first step, the temperature of refluxing extraction is 75 DEG C
To 90 DEG C.
4. according to claim 1 and 2 to pierce sugared polysaccharide, it is characterised in that in the 3rd step, dwell temperature is 5 DEG C to 8 DEG C.
5. according to claim 3 to pierce sugared polysaccharide, it is characterised in that in the 3rd step, dwell temperature is 5 DEG C to 8 DEG C.
6. a kind of preparation method of the sugared polysaccharide of thorn according to claim 1, it is characterised in that carry out in the steps below：First
Step, will obtain piercing Icing Sugar after piercing sugared medicinal material drying and crushing, Icing Sugar and petroleum ether backflow degreasing then will be pierced, by the thorn after degreasing
Icing Sugar obtains Aqueous extracts after carrying out refluxing extraction with distilled water, wherein, thorn Icing Sugar is 1 with the volume ratio of distilled water:7 to 9；Second
Step, obtains piercing sugared concentrate after 1/to five/6th that Aqueous extracts are concentrated under reduced pressure into Aqueous extracts volume；3rd step, to
The ethanol water that 95% is added in the sugared concentrate of thorn is 80% to 90% up to piercing the alcohol precipitation concentration in sugared concentrate, will add 95%
Ethanol water thorn sugar concentrate stand 20h to 24h after obtain stand liquid；4th step, will stand after liquid carries out suction filtration and obtain
To filter cake, filter cake sequentially is obtained piercing sugared polysaccharide by after absolute ethanol washing, acetone washing, vacuum drying.
7. the preparation method of the sugared polysaccharide of thorn according to claim 6, it is characterised in that in the first step, the time of refluxing extraction
For 1h to 2h.
8. the preparation method of the sugared polysaccharide of thorn according to claim 6 or 7, it is characterised in that in the first step, refluxing extraction
Temperature is 75 DEG C to 90 DEG C.
9. the preparation method of the sugared polysaccharide of thorn according to claim 6 or 7, it is characterised in that in the 3rd step, dwell temperature is 5
DEG C to 8 DEG C.
10. the preparation method of the sugared polysaccharide of thorn according to claim 8, it is characterised in that in the 3rd step, dwell temperature is 5 DEG C
To 8 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201710105241.6A CN106749745A (en) | 2017-02-25 | 2017-02-25 | Sugared polysaccharide of thorn and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710105241.6A CN106749745A (en) | 2017-02-25 | 2017-02-25 | Sugared polysaccharide of thorn and preparation method thereof |
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| CN106749745A true CN106749745A (en) | 2017-05-31 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107602632A (en) * | 2017-09-28 | 2018-01-19 | 新疆医科大学 | Pierce sugared FOS extract and its extracting method |
| CN111410698A (en) * | 2020-03-13 | 2020-07-14 | 广东药科大学 | Camel thorn sugar polymer and preparation method and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103040918A (en) * | 2012-12-28 | 2013-04-17 | 新疆维吾尔自治区中药民族药研究所 | Alhagi pseudoalhagi extract capsule and preparation method thereof |
-
2017
- 2017-02-25 CN CN201710105241.6A patent/CN106749745A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103040918A (en) * | 2012-12-28 | 2013-04-17 | 新疆维吾尔自治区中药民族药研究所 | Alhagi pseudoalhagi extract capsule and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 菅丽君等: "刺糖多糖的分离纯化与基础结构分析", 《新疆医科大学学报》 * |
| 菅丽君等: "刺糖多糖的分离纯化与结构分析", 《中国药学杂志》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107602632A (en) * | 2017-09-28 | 2018-01-19 | 新疆医科大学 | Pierce sugared FOS extract and its extracting method |
| CN107602632B (en) * | 2017-09-28 | 2021-02-02 | 新疆医科大学 | Extract of fructo-oligosaccharide and its extraction method |
| CN111410698A (en) * | 2020-03-13 | 2020-07-14 | 广东药科大学 | Camel thorn sugar polymer and preparation method and application thereof |
| CN111410698B (en) * | 2020-03-13 | 2022-01-04 | 广东药科大学 | Camel thorn sugar polymer and preparation method and application thereof |
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Application publication date: 20170531 |