CN106749726A - A kind of method for extracting Arillus longan polysaccharide - Google Patents
A kind of method for extracting Arillus longan polysaccharide Download PDFInfo
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- CN106749726A CN106749726A CN201611148241.6A CN201611148241A CN106749726A CN 106749726 A CN106749726 A CN 106749726A CN 201611148241 A CN201611148241 A CN 201611148241A CN 106749726 A CN106749726 A CN 106749726A
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- Prior art keywords
- arillus longan
- mixed
- solution
- longan polysaccharide
- enzymolysis
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- 235000000235 Euphoria longan Nutrition 0.000 title claims abstract description 76
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 61
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 61
- 238000000034 method Methods 0.000 title claims abstract description 44
- 150000004676 glycans Chemical class 0.000 title claims abstract 12
- 244000139609 Euphoria longan Species 0.000 title 1
- 240000001008 Dimocarpus longan Species 0.000 claims abstract description 75
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 45
- 102000004190 Enzymes Human genes 0.000 claims abstract description 34
- 108090000790 Enzymes Proteins 0.000 claims abstract description 34
- 239000003513 alkali Substances 0.000 claims abstract description 33
- 239000003054 catalyst Substances 0.000 claims abstract description 31
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 28
- 239000007788 liquid Substances 0.000 claims abstract description 26
- 150000001875 compounds Chemical class 0.000 claims abstract description 25
- 230000003213 activating effect Effects 0.000 claims abstract description 19
- 239000012141 concentrate Substances 0.000 claims abstract description 18
- 239000002244 precipitate Substances 0.000 claims abstract description 16
- 238000000605 extraction Methods 0.000 claims abstract description 14
- 238000000108 ultra-filtration Methods 0.000 claims abstract description 13
- 238000001994 activation Methods 0.000 claims abstract description 12
- 239000000706 filtrate Substances 0.000 claims abstract description 9
- 238000001694 spray drying Methods 0.000 claims abstract description 9
- MCMNRKCIXSYSNV-UHFFFAOYSA-N Zirconium dioxide Chemical compound O=[Zr]=O MCMNRKCIXSYSNV-UHFFFAOYSA-N 0.000 claims abstract description 4
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 82
- 239000000243 solution Substances 0.000 claims description 58
- 229940088598 enzyme Drugs 0.000 claims description 32
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims description 24
- YUTHOFNNBDODRQ-UHFFFAOYSA-N oxocopper oxygen(2-) zirconium(4+) Chemical compound [O-2].[Zr+4].[Cu]=O.[O-2] YUTHOFNNBDODRQ-UHFFFAOYSA-N 0.000 claims description 20
- 230000001476 alcoholic effect Effects 0.000 claims description 16
- 238000002156 mixing Methods 0.000 claims description 15
- 239000008367 deionised water Substances 0.000 claims description 14
- 229910021641 deionized water Inorganic materials 0.000 claims description 14
- 238000003756 stirring Methods 0.000 claims description 14
- 238000006243 chemical reaction Methods 0.000 claims description 11
- 235000011114 ammonium hydroxide Nutrition 0.000 claims description 10
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 claims description 10
- 108091005804 Peptidases Proteins 0.000 claims description 9
- 239000004365 Protease Substances 0.000 claims description 9
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 9
- 230000002378 acidificating effect Effects 0.000 claims description 9
- 230000035484 reaction time Effects 0.000 claims description 9
- 239000007787 solid Substances 0.000 claims description 9
- 239000007864 aqueous solution Substances 0.000 claims description 8
- 238000000889 atomisation Methods 0.000 claims description 8
- 238000001816 cooling Methods 0.000 claims description 8
- 238000001035 drying Methods 0.000 claims description 8
- 230000007071 enzymatic hydrolysis Effects 0.000 claims description 8
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 claims description 8
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 claims description 8
- 238000001914 filtration Methods 0.000 claims description 8
- XTUSEBKMEQERQV-UHFFFAOYSA-N propan-2-ol;hydrate Chemical compound O.CC(C)O XTUSEBKMEQERQV-UHFFFAOYSA-N 0.000 claims description 8
- 238000005292 vacuum distillation Methods 0.000 claims description 8
- 230000008859 change Effects 0.000 claims description 6
- 108010059892 Cellulase Proteins 0.000 claims description 3
- 229940106157 cellulase Drugs 0.000 claims description 3
- 239000001301 oxygen Substances 0.000 claims description 3
- 229910052760 oxygen Inorganic materials 0.000 claims description 3
- 230000000717 retained effect Effects 0.000 claims description 3
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 claims description 2
- 229910000366 copper(II) sulfate Inorganic materials 0.000 claims description 2
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 claims 1
- QCWXUUIWCKQGHC-UHFFFAOYSA-N Zirconium Chemical compound [Zr] QCWXUUIWCKQGHC-UHFFFAOYSA-N 0.000 claims 1
- WGKMWBIFNQLOKM-UHFFFAOYSA-N [O].[Cl] Chemical compound [O].[Cl] WGKMWBIFNQLOKM-UHFFFAOYSA-N 0.000 claims 1
- 229910052726 zirconium Inorganic materials 0.000 claims 1
- 238000002360 preparation method Methods 0.000 abstract description 33
- 238000005516 engineering process Methods 0.000 abstract description 9
- 239000000047 product Substances 0.000 abstract description 7
- 230000004913 activation Effects 0.000 abstract description 3
- 238000009776 industrial production Methods 0.000 abstract description 2
- QPLDLSVMHZLSFG-UHFFFAOYSA-N Copper oxide Chemical compound [Cu]=O QPLDLSVMHZLSFG-UHFFFAOYSA-N 0.000 abstract 2
- 229960004643 cupric oxide Drugs 0.000 abstract 1
- 150000004804 polysaccharides Chemical class 0.000 description 49
- 229920002678 cellulose Polymers 0.000 description 16
- 239000001913 cellulose Substances 0.000 description 16
- 239000000203 mixture Substances 0.000 description 13
- 230000000052 comparative effect Effects 0.000 description 9
- 239000000463 material Substances 0.000 description 9
- 230000033228 biological regulation Effects 0.000 description 7
- 238000007254 oxidation reaction Methods 0.000 description 7
- 108010084185 Cellulases Proteins 0.000 description 6
- 102000005575 Cellulases Human genes 0.000 description 6
- NZSLBYVEIXCMBT-UHFFFAOYSA-N chloro hypochlorite;zirconium Chemical class [Zr].ClOCl NZSLBYVEIXCMBT-UHFFFAOYSA-N 0.000 description 6
- 238000002242 deionisation method Methods 0.000 description 6
- 239000002002 slurry Substances 0.000 description 6
- 150000003467 sulfuric acid derivatives Chemical class 0.000 description 6
- 239000003643 water by type Substances 0.000 description 6
- 108090000765 processed proteins & peptides Proteins 0.000 description 5
- 238000005406 washing Methods 0.000 description 5
- 235000013399 edible fruits Nutrition 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 3
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 3
- WMFOQBRAJBCJND-UHFFFAOYSA-M Lithium hydroxide Chemical compound [Li+].[OH-] WMFOQBRAJBCJND-UHFFFAOYSA-M 0.000 description 3
- 229920002472 Starch Polymers 0.000 description 3
- 239000013078 crystal Substances 0.000 description 3
- 239000001257 hydrogen Substances 0.000 description 3
- 229910052739 hydrogen Inorganic materials 0.000 description 3
- RVTZCBVAJQQJTK-UHFFFAOYSA-N oxygen(2-);zirconium(4+) Chemical compound [O-2].[O-2].[Zr+4] RVTZCBVAJQQJTK-UHFFFAOYSA-N 0.000 description 3
- 229920002401 polyacrylamide Polymers 0.000 description 3
- 229920001184 polypeptide Polymers 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 102000004196 processed proteins & peptides Human genes 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 239000002904 solvent Substances 0.000 description 3
- 239000008107 starch Substances 0.000 description 3
- 235000019698 starch Nutrition 0.000 description 3
- 229910001928 zirconium oxide Inorganic materials 0.000 description 3
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- RKTYLMNFRDHKIL-UHFFFAOYSA-N copper;5,10,15,20-tetraphenylporphyrin-22,24-diide Chemical group [Cu+2].C1=CC(C(=C2C=CC([N-]2)=C(C=2C=CC=CC=2)C=2C=CC(N=2)=C(C=2C=CC=CC=2)C2=CC=C3[N-]2)C=2C=CC=CC=2)=NC1=C3C1=CC=CC=C1 RKTYLMNFRDHKIL-UHFFFAOYSA-N 0.000 description 2
- 239000002537 cosmetic Substances 0.000 description 2
- 238000004090 dissolution Methods 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 239000000446 fuel Substances 0.000 description 2
- 230000007062 hydrolysis Effects 0.000 description 2
- 238000006460 hydrolysis reaction Methods 0.000 description 2
- 235000013372 meat Nutrition 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- WGYKZJWCGVVSQN-UHFFFAOYSA-N propylamine Chemical compound CCCN WGYKZJWCGVVSQN-UHFFFAOYSA-N 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- ZGXJTSGNIOSYLO-UHFFFAOYSA-N 88755TAZ87 Chemical compound NCC(=O)CCC(O)=O ZGXJTSGNIOSYLO-UHFFFAOYSA-N 0.000 description 1
- 102000004400 Aminopeptidases Human genes 0.000 description 1
- 108090000915 Aminopeptidases Proteins 0.000 description 1
- 241000208340 Araliaceae Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- 102000005367 Carboxypeptidases Human genes 0.000 description 1
- 108010006303 Carboxypeptidases Proteins 0.000 description 1
- SHZGCJCMOBCMKK-UHFFFAOYSA-N D-mannomethylose Natural products CC1OC(O)C(O)C(O)C1O SHZGCJCMOBCMKK-UHFFFAOYSA-N 0.000 description 1
- 102000005593 Endopeptidases Human genes 0.000 description 1
- 108010059378 Endopeptidases Proteins 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 241000165940 Houjia Species 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- SHZGCJCMOBCMKK-JFNONXLTSA-N L-rhamnopyranose Chemical compound C[C@@H]1OC(O)[C@H](O)[C@H](O)[C@H]1O SHZGCJCMOBCMKK-JFNONXLTSA-N 0.000 description 1
- PNNNRSAQSRJVSB-UHFFFAOYSA-N L-rhamnose Natural products CC(O)C(O)C(O)C(O)C=O PNNNRSAQSRJVSB-UHFFFAOYSA-N 0.000 description 1
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical class CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 1
- NIPNSKYNPDTRPC-UHFFFAOYSA-N N-[2-oxo-2-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)ethyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 NIPNSKYNPDTRPC-UHFFFAOYSA-N 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 230000018199 S phase Effects 0.000 description 1
- 241001093760 Sapindaceae Species 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 241000223259 Trichoderma Species 0.000 description 1
- 125000000738 acetamido group Chemical group [H]C([H])([H])C(=O)N([H])[*] 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000003905 agrochemical Substances 0.000 description 1
- 239000012670 alkaline solution Substances 0.000 description 1
- SRBFZHDQGSBBOR-QMKXCQHVSA-N alpha-L-arabinopyranose Chemical compound O[C@H]1CO[C@@H](O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-QMKXCQHVSA-N 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 1
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000012459 cleaning agent Substances 0.000 description 1
- 239000000701 coagulant Substances 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 229920001577 copolymer Polymers 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 239000012024 dehydrating agents Substances 0.000 description 1
- 239000000645 desinfectant Substances 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 238000001976 enzyme digestion Methods 0.000 description 1
- 239000000686 essence Substances 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 230000003311 flocculating effect Effects 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 229940118199 levulan Drugs 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- YKYONYBAUNKHLG-UHFFFAOYSA-N n-Propyl acetate Natural products CCCOC(C)=O YKYONYBAUNKHLG-UHFFFAOYSA-N 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 150000004880 oxines Chemical class 0.000 description 1
- CMOAHYOGLLEOGO-UHFFFAOYSA-N oxozirconium;dihydrochloride Chemical compound Cl.Cl.[Zr]=O CMOAHYOGLLEOGO-UHFFFAOYSA-N 0.000 description 1
- 239000003973 paint Substances 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 239000002304 perfume Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- CHWRSCGUEQEHOH-UHFFFAOYSA-N potassium oxide Chemical compound [O-2].[K+].[K+] CHWRSCGUEQEHOH-UHFFFAOYSA-N 0.000 description 1
- 229910001950 potassium oxide Inorganic materials 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 229940090181 propyl acetate Drugs 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- MUPFEKGTMRGPLJ-ZQSKZDJDSA-N raffinose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO[C@@H]2[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O2)O)O1 MUPFEKGTMRGPLJ-ZQSKZDJDSA-N 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011949 solid catalyst Substances 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 235000013599 spices Nutrition 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- NQPDZGIKBAWPEJ-UHFFFAOYSA-N valeric acid Chemical compound CCCCC(O)=O NQPDZGIKBAWPEJ-UHFFFAOYSA-N 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 238000003809 water extraction Methods 0.000 description 1
- 238000009941 weaving Methods 0.000 description 1
- 229920001221 xylan Polymers 0.000 description 1
- 150000004823 xylans Chemical class 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Sustainable Development (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a kind of method for extracting Arillus longan polysaccharide, comprise the following steps：(1) slurrying and activation process：Fresh arillus longan and pure water are mixed, slurrying in homogenizer is poured into, alkali lye is subsequently adding, 10 12h are activated at minus 4 10 DEG C, obtain activating solution；(2) enzymolysis and concentration：The pH value of activating solution is adjusted, cupric oxide zirconia catalyst and tri compound enzyme are then added in filtrate, digested under conditions of 200 500W microwaves, then the enzymolysis liquid obtained after enzymolysis is concentrated by ultrafiltration, obtain concentrate；(3) centrifugal treating：Concentrate and mixed alcohol solution are mixed, centrifugal collection precipitate is used；(4) spray drying treatment.The present invention is assisted the extraction preparation process of technology transfer longan polysaccharide by alkali lye low-temperature activation enzyme, and the yield of target product is significantly improved than routine techniques, with it is efficient, quick, inexpensive the characteristics of, be suitable for large-scale industrial production.
Description
【Technical field】
The present invention relates to the field of deep of longan, more particularly to a kind of method for extracting Arillus longan polysaccharide.
【Background technology】
Longan is the fruit of Sapindaceae, also known as longan is the famous subtropical fruit of China.Longan originates in China south
Portion and the south subtropicses region of North Vietnam, are the evergreen length of important south subtropicses in existing more than the 2000 years cultivation history of China
Longevity fruit tree, the in history title of south " longan " north " ginseng ".The situation of selling well of longan fruit is enjoyed at home and abroad higher in the international market
Reputation, China's Longan Cultivation area and yield occupy first place in the world, and it is main producing region that China's Fujian Guangdong, Guangxi and Taiwan etc. are saved, sea
Southern Yunnan Sichuan and Guizhou etc. save also cultivation.
Arillus longan polysaccharide is generally tan amorphous solid, easily the moisture absorption, and white powder is obtained after isolating and purifying,
Insoluble in organic solvents such as ethanol, acetone, n-butanols, it is more difficult to be dissolved in cold water, its be mainly by rhamnose, glucose, galactolipin,
The Type B pyrans acid heteroglycan of the tool acetylamino structure of the compositions such as xylose, pectinose is constituted.At present, both at home and abroad for dragon
Using conventional hot water's extraction, polysaccharide material to be difficult to be discharged from longan pulp is intracellular the research of eye meat polysaccharide extracting process more
Come, cause yield relatively low；Additionally, Hot water extraction requirement extraction time is long, Extracting temperature is high, easily causes polysaccharide hydrolysis, reduces
Polysaccharide material bioactivity.Enzymatic isolation method is one of method of effective component extracting from plant to greatest extent, and enzyme digestion reaction was both
More leniently plant tissue can be decomposed, yield is improved by a relatively large margin；It is also less prone to destroy stereochemical structure and the life of polysaccharide simultaneously
Thing activity, has been increasingly becoming the focus of active components of plants Study on extraction in recent years.The extractive technique of existing longan polysaccharide
Longer time is needed, high cost, the efficiency for separating preparation are low.Therefore, recovery rate, the product yield of longan polysaccharide how to be improved
With the bottleneck problem that product purity has turned into longan polysaccharide industrialization urgent need to resolve.
String is pre-processed in low temperature alkali lye, interaction of hydrogen bond, destruction can occur by alkali lye and cellulose
The crystal structure of cellulose, so as to increase dissolubility of the cellulose in water.Wuhan University Zhang Lina establishes the low of cellulose
Warm dissolving technology (publication number CN200710052532.X, CN200710051867.X), the method is with lithium hydroxide or Strong oxdiative
Sodium, urea, water are raw material, and molecular weight is realized under cryogenic less than 30 × 104Native cellulose or II fiber types element
Dissolving, the course of dissolution is physical process, is chemically reacted, the easy recycling use of waste liquid, with wider
Application prospect.
【The content of the invention】
Present invention aim at a kind of method for extracting Arillus longan polysaccharide is provided, alkali lye low-temperature activation enzyme is assisted skill by the technology
Art introduces the extraction preparation process of longan polysaccharide, and the yield of target product is significantly improved than routine techniques, with efficient, fast
The characteristics of speed, low cost, it is suitable for large-scale industrial production.
To reach above-mentioned purpose, the technical solution adopted in the present invention is：A kind of method for extracting Arillus longan polysaccharide, including
Following steps：
(1) slurrying and activation process：Fresh arillus longan and pure water are mixed, slurrying in homogenizer, Ran Houjia is poured into
Enter alkali lye, 10-12h is activated at negative 4-10 DEG C, obtain activating solution；
(2) enzymolysis and concentration：The pH value of activating solution is adjusted, copper oxide-zirconium oxide catalysis is then added in filtrate
Agent and tri compound enzyme, are digested under conditions of 200-500W microwaves, when the reaction temperature of controlled enzymatic hydrolysis and reaction
Between, then the enzymolysis liquid obtained after enzymolysis is concentrated by ultrafiltration, obtain concentrate；
(3) centrifugal treating：Concentrate and mixed alcohol solution are mixed, after ultrasonically treated 25-40min, is received with centrifuge
Collection precipitate；
(4) spray drying treatment：Precipitate is washed with 85-95 DEG C of deionized water, rotation throwing disc is put into after cooling and is turned
Speed obtains Arillus longan polysaccharide to dry 10-30s in the rotation type atomization drier of 15000-25000r/min.
In the present invention, as further illustrating, the copper oxide-zirconium oxide catalyst described in step (2) is by following methods
Prepare：Count by weight, 35-50 parts of anhydrous cupric sulfate, 30-45 parts of zirconium oxychloride are added to 200-300 parts of deionized water
In, it is the sodium hydroxide solution of 40-50% that mass fraction is added dropwise while stirring, and regulation pH value is 9-12, and filtering takes filter residue, uses
Deionized water is washed 2-4 times, is dried, microwave drying 10-20min, finally with 1100-1300 DEG C of roasting 2-4h in Muffle furnace,
Obtain copper oxide-zirconium oxide catalyst.
In the present invention, as further illustrating, the alkali lye described in step (1) is by weight 1：1-3：The hydrogen of 10-15
Potassium oxide, mass fraction are that 25% ammoniacal liquor and deionized water are mixed.
In the present invention, as further illustrating, the tri compound enzyme described in step (2) is by weight 50-70：20-
35：The acidic cellulase of 15-30, fructosidase and protease are mixed.
In the present invention, as further illustrating, the pH value described in step (2) is 4.5-5.5, and described reaction temperature is
50-75 DEG C, the described reaction time is 40-60min.
In the present invention, as further illustrating, the ultrafiltration concentration described in step (2) is to retain 5-50KD using milipore filter
The enzymolysis liquid of molecular weight, temperature be 40-60 DEG C, pressure be -0.09MPa under conditions of vacuum distillation to solid content be
80-95%.
In the present invention, as further illustrating, the mixing alcoholic solution described in step (3) is by weight 10-16：1：1-
2 mass fraction be 95% ethanol water, mass fraction for 70-80% the normal propyl alcohol aqueous solution and mass fraction be 70-
90% isopropanol water solution is mixed.
In the present invention, as further illustrating, described fresh arillus longan, pure water, alkali lye, copper oxide-zirconium oxide
The weight ratio of catalyst, tri compound enzyme and mixing alcoholic solution is 100：250-500：20-40：1-3：5-8：500-700.Part
The function introduction of raw material is as follows：
Starch branch polyacrylamide flocculant, is the copolymer of starch grafted polyacrylamide, is divided into gel-type and line style
Two kinds of grafted chain, is a kind of new high polymer coagulant, and it combines the characteristic of starch and polyacrylamide, with good
Flocculating property.
Acidic cellulase, is obtained by refined extraction of liquid submerged fermentation by koning trichoderma bacterium.Product extensive use
In industries such as weaving, feed, alcohol, alcohol fuel, brewing, traditional Chinese medicine extractions.
Fructosidase, a kind of ectoenzyme, the levulan molecule that specifically catalyzing hydrolysis are made up of β-D-Fructose can also water
Solution synanthrin, sucrose, gossypose etc..
Protease is the general name of the class of enzymes of aminosal peptide chain.It is divided into endopeptidase and end in the way of it degrades polypeptide
The class of peptase two.The former can cut off the polypeptide chain of macromolecule from centre, form the less protein of molecular weight and peptone；The latter can divide again
It is carboxypeptidase and aminopeptidase, peptide chain is hydrolyzed generate respectively by they one by one from the free carboxy termini or free amine end of polypeptide
Amino acid.
Ethanol, is commonly called as alcohol, and it is a kind of inflammable, volatile colourless transparent liquid under normal temperature, normal pressure, it water-soluble
Liquid has special, pleasant fragrance, and slightly excitant.Having many uses for ethanol, can manufacture acetic acid, drink with ethanol
Material, essence, dyestuff, fuel etc..Volume fraction is medically also commonly used for the ethanol of 70%-75% makees disinfectant etc..
Normal propyl alcohol, colourless transparent liquid, 97.15 DEG C of boiling point.Water is dissolved in, it is inflammable.It is mainly used as in solvent and organic synthesis
Mesosome.In the U.S., normal propyl alcohol about 40% is directly used as solvent or synthesis propyl acetate, for paint solvent, printing-ink, cosmetic
Product etc..40% is used to produce medicine, the intermediate n-propylamine of agricultural chemicals.20% is used to produce feed addictive, synthetic perfume etc..
Isopropanol, colourless transparent liquid is dissolved in the organic solvents such as water, alcohol, ether, benzene, is important chemical products and raw material,
It is mainly used in being used as dehydrating agent and cleaning agent in pharmacy, cosmetics, plastics, spices and coating and electronics industry.
The invention has the advantages that：
1. the present invention uses alkali lye low-temperature activation technology, can be interacted by alkali lye and cellulose, destroys cellulose
Crystal structure, so as to increase dissolubility of the cellulose in water, the dissolution rate of Arillus longan polysaccharide can be effectively improved.Institute of the present invention
Ammoniacal liquor in the alkali lye of use can effectively break the unstable chemical bond between cellulose in arillus longan, and ammoniacal liquor acts on fibre
Dimension element causes its swelling and crystalline solid phase transformation, and ammoniacal liquorization can significantly increase the porosity of biomass, increases cellulose to enzyme
Absorption, increase the accessible area of cellulose and enzyme, so as to improve enzymolysis speed；Potassium hydroxide can dissolve pulp,
Make pulp short texture, moreover it is possible to break the hydrogen bond between cellulose, reduce the crystallinity of cellulose, increase cellulose can
And property is so as to improve enzymatic hydrolyzation；On the other hand, under the conditions of cryogenic freezing, become big into the molecular structure water inside pulp, directly
Connect from inside and strut pulp organization, make the structure of pulp by closely becoming loose, so as in increased pulp
The accessible area of cellulose and enzyme, improves enzymolysis efficiency, further improves the yield of Arillus longan polysaccharide；This three's phase interworking
Close, interact, it is indispensable, enzymolysis efficiency is improved from different perspectives, the yield of the pulp polysaccharide of acquirement is far above single bar
The yield of the Arillus longan polysaccharide produced by part.
2. copper oxide-zirconium oxide catalyst is prepared invention applies the precipitation method, the technology is sunk by alkaline solution
Shallow lake copper oxide-zirconium oxide catalyst, by microwave treatment, can make the internal structure of copper oxide-zirconium oxide catalyst in it is loose,
Cellular, finally fixes the shape of cupric oxide-zirconia catalyst by 1100-1300 DEG C of high-temperature roasting again, because
At 1100 DEG C, the crystal formation of zirconium oxide is transformed into tetragonal structure from monoclinic form, and the zirconium oxide of tetragonal structure can more excite enzyme
Zirconium oxide is fixed at a temperature of activity, promotes to digest, therefore its effect for producing is far above 400-600 DEG C that prior art is used
Effect, and the copper oxide-zirconium oxide catalyst prepared by the precipitation method can be effectively increased and tri compound enzyme
Contact area, further improves enzymolysis efficiency.
3. the ultrafiltration concentration process that the present invention is used applies Ultra filtration membrane technology, and selective retention molecular weight is 5-50KD
Enzymolysis liquid, while effectively catching Arillus longan polysaccharide, in eliminating cellulose, xylan, the extract for digesting
Small molecular weight impurity and production in extra addition complex enzyme, significantly improve the purity of Arillus longan polysaccharide.
【Specific embodiment】
Embodiment 1：
1. early-stage preparations：
The preparation of copper oxide-zirconium oxide catalyst：35g anhydrous cupric sulfates, 30g zirconium oxychlorides are added to 200g deionizations
In water, while stirring be added dropwise mass fraction be 40% sodium hydroxide solution, regulation pH value be 9, filtering, take filter residue, spend from
Sub- water washing 2 times, dries, microwave drying 10min, finally obtains copper oxide-zirconium oxide with 1100 DEG C of roasting 2h in Muffle furnace
Catalyst.
The preparation of alkali lye：10g potassium hydroxide, the ammoniacal liquor that 10g mass fractions are 25% and 100g deionized waters are mixed, is stirred
Mix and uniformly obtain alkali lye.
The preparation of tri compound enzyme：50g acidic cellulases, 20g fructosidases and 15g protease are well mixed, are obtained
To tri compound enzyme.
Mix the preparation of alcoholic solution：It is 70% by ethanol water that 1000g mass fractions are 95%, 100g mass fractions
The normal propyl alcohol aqueous solution and the mixing of isopropanol water solution that 100g mass fractions are 70%, stir, obtain mixing alcoholic solution.
The method that the material that above-mentioned early stage is prepared is used for following extraction Arillus longan polysaccharides.
2. a kind of method for extracting Arillus longan polysaccharide, comprises the following steps：
(1) slurrying and activation process：The fresh arillus longans of 100g and 250g pure water are mixed, is poured into homogenizer and is made
Slurry, is subsequently adding 20g alkali lye, and 10h is activated at minus 4 DEG C, obtains activating solution；
(2) enzymolysis and concentration：The pH value for adjusting activating solution is 4.5, and 1g cupric oxide-oxidation is then added in filtrate
Zr catalyst and 5g tri compound enzymes, are digested under conditions of 200W microwaves, and the reaction temperature of controlled enzymatic hydrolysis is 50
DEG C and the reaction time be 40min, then the enzymolysis liquid obtained after enzymolysis is concentrated by ultrafiltration, retain 5-50KD point with milipore filter
Son amount enzymolysis liquid, temperature be 40 DEG C, pressure be -0.09MPa under conditions of vacuum distillation to solid content be 80%, obtain
To concentrate；
(3) centrifugal treating：Concentrate and 500g mixed alcohol solution are mixed, after ultrasonically treated 25min, is received with centrifuge
Collection precipitate；
(4) spray drying treatment：Precipitate is washed with 85 DEG C of deionized water, rotation throwing disc rotating speed is put into after cooling is
10s is dried in the rotation type atomization drier of 15000r/min, Arillus longan polysaccharide is obtained.
Embodiment 2：
1. early-stage preparations：
The preparation of copper oxide-zirconium oxide catalyst：38g anhydrous cupric sulfates, 35g zirconium oxychlorides are added to 240g deionizations
In water, while stirring be added dropwise mass fraction be 45% sodium hydroxide solution, regulation pH value be 10, filtering, take filter residue, spend from
Sub- water washing 3 times, dries, microwave drying 13min, finally obtains cupric oxide-oxidation with 1200 DEG C of roasting 2.5h in Muffle furnace
Zr catalyst.
The preparation of alkali lye：10g potassium hydroxide, the ammoniacal liquor that 18g mass fractions are 25% and 120g deionized waters are mixed, is stirred
Mix and uniformly obtain alkali lye.
The preparation of tri compound enzyme：65g acidic cellulases, 23g fructosidases and 20g protease are well mixed, are obtained
To tri compound enzyme.
Mix the preparation of alcoholic solution：It is 75% by ethanol water that 1200g mass fractions are 95%, 100g mass fractions
The normal propyl alcohol aqueous solution and the mixing of isopropanol water solution that 133g mass fractions are 78%, stir, obtain mixing alcoholic solution.
The method that the material that above-mentioned early stage is prepared is used for following extraction Arillus longan polysaccharides.
2. a kind of method for extracting Arillus longan polysaccharide, comprises the following steps：
(1) slurrying and activation process：The fresh arillus longans of 100g and 400g pure water are mixed, is poured into homogenizer and is made
Slurry, is subsequently adding 33g alkali lye, and 10.5h is activated at minus 5 DEG C, obtains activating solution；
(2) enzymolysis and concentration：The pH value for adjusting activating solution is 5, and 1.5g cupric oxide-oxidation is then added in filtrate
Zr catalyst and 6g tri compound enzymes, are digested under conditions of 400W microwaves, and the reaction temperature of controlled enzymatic hydrolysis is 63
DEG C and the reaction time be 51min, then the enzymolysis liquid obtained after enzymolysis is concentrated by ultrafiltration, retain 5-50KD point with milipore filter
Son amount enzymolysis liquid, temperature be 49 DEG C, pressure be -0.09MPa under conditions of vacuum distillation to solid content be 87%, obtain
To concentrate；
(3) centrifugal treating：Concentrate and 650g mixed alcohol solution are mixed, after ultrasonically treated 33min, is received with centrifuge
Collection precipitate；
(4) spray drying treatment：Precipitate is washed with 90 DEG C of deionized water, rotation throwing disc rotating speed is put into after cooling is
19s is dried in the rotation type atomization drier of 18000r/min, Arillus longan polysaccharide is obtained.
Embodiment 3：
1. early-stage preparations：
The preparation of copper oxide-zirconium oxide catalyst：43g anhydrous cupric sulfates, 37g zirconium oxychlorides are added to 260g deionizations
In water, while stirring be added dropwise mass fraction be 44% sodium hydroxide solution, regulation pH value be 11, filtering, take filter residue, spend from
Sub- water washing 3 times, dries, microwave drying 15min, finally obtains copper oxide-zirconium oxide with 1150 DEG C of roasting 3h in Muffle furnace
Catalyst.
The preparation of alkali lye：10g potassium hydroxide, the ammoniacal liquor that 24g mass fractions are 25% and 130g deionized waters are mixed, is stirred
Mix and uniformly obtain alkali lye.
The preparation of tri compound enzyme：66g acidic cellulases, 30g fructosidases and 26g protease are well mixed, are obtained
To tri compound enzyme.
Mix the preparation of alcoholic solution：It is 75% by ethanol water that 1450g mass fractions are 95%, 100g mass fractions
The normal propyl alcohol aqueous solution and the mixing of isopropanol water solution that 170g mass fractions are 82%, stir, obtain mixing alcoholic solution.
The method that the material that above-mentioned early stage is prepared is used for following extraction Arillus longan polysaccharides.
2. a kind of method for extracting Arillus longan polysaccharide, comprises the following steps：
(1) slurrying and activation process：The fresh arillus longans of 100g and 360g pure water are mixed, is poured into homogenizer and is made
Slurry, is subsequently adding 26g alkali lye, and 11.5h is activated at minus 6 DEG C, obtains activating solution；
(2) enzymolysis and concentration：The pH value for adjusting activating solution is 5, and 1.5g cupric oxide-oxidation is then added in filtrate
Zr catalyst and 7g tri compound enzymes, are digested under conditions of 390W microwaves, and the reaction temperature of controlled enzymatic hydrolysis is 60
DEG C and the reaction time be 54min, then the enzymolysis liquid obtained after enzymolysis is concentrated by ultrafiltration, retain 5-50KD point with milipore filter
Son amount enzymolysis liquid, temperature be 49 DEG C, pressure be -0.09MPa under conditions of vacuum distillation to solid content be 92%, obtain
To concentrate；
(3) centrifugal treating：Concentrate and 640g mixed alcohol solution are mixed, after ultrasonically treated 36min, is received with centrifuge
Collection precipitate；
(4) spray drying treatment：Precipitate is washed with 91 DEG C of deionized water, rotation throwing disc rotating speed is put into after cooling is
22s is dried in the rotation type atomization drier of 20000r/min, Arillus longan polysaccharide is obtained.
Embodiment 4：
1. early-stage preparations：
The preparation of copper oxide-zirconium oxide catalyst：47g anhydrous cupric sulfates, 40g zirconium oxychlorides are added to 255g deionizations
In water, while stirring be added dropwise mass fraction be 43% sodium hydroxide solution, regulation pH value be 10, filtering, take filter residue, spend from
Sub- water washing 2 times, dries, microwave drying 13min, finally obtains cupric oxide-oxidation with 1250 DEG C of roasting 3.5h in Muffle furnace
Zr catalyst.
The preparation of alkali lye：10g potassium hydroxide, the ammoniacal liquor that 25g mass fractions are 25% and 110g deionized waters are mixed, is stirred
Mix and uniformly obtain alkali lye.
The preparation of tri compound enzyme：63g acidic cellulases, 24g fructosidases and 28g protease are well mixed, are obtained
To tri compound enzyme.
Mix the preparation of alcoholic solution：It is 73% by ethanol water that 1500g mass fractions are 95%, 100g mass fractions
The normal propyl alcohol aqueous solution and the mixing of isopropanol water solution that 140g mass fractions are 84%, stir, obtain mixing alcoholic solution.
The method that the material that above-mentioned early stage is prepared is used for following extraction Arillus longan polysaccharides.
2. a kind of method for extracting Arillus longan polysaccharide, comprises the following steps：
(1) slurrying and activation process：The fresh arillus longans of 100g and 360g pure water are mixed, is poured into homogenizer and is made
Slurry, is subsequently adding 24g alkali lye, and 10h is activated at minus 7 DEG C, obtains activating solution；
(2) enzymolysis and concentration：The pH value for adjusting activating solution is 4.5, and 2.5g cupric oxide-oxygen is then added in filtrate
Change Zr catalyst and 6.5g tri compound enzymes, digested under conditions of 300W microwaves, the reaction temperature of controlled enzymatic hydrolysis
It is 55min with the reaction time to be 66 DEG C, and then the enzymolysis liquid obtained after enzymolysis is concentrated by ultrafiltration, and 5- is retained with milipore filter
The enzymolysis liquid of 50KD molecular weight, temperature be 52 DEG C, pressure be -0.09MPa under conditions of vacuum distillation to solid content be
87%, obtain concentrate；
(3) centrifugal treating：Concentrate and 580g mixed alcohol solution are mixed, after ultrasonically treated 32min, is received with centrifuge
Collection precipitate；
(4) spray drying treatment：Precipitate is washed with 90 DEG C of deionized water, rotation throwing disc rotating speed is put into after cooling is
23s is dried in the rotation type atomization drier of 19000r/min, Arillus longan polysaccharide is obtained.
Embodiment 5：
1. early-stage preparations：
The preparation of copper oxide-zirconium oxide catalyst：44g anhydrous cupric sulfates, 36g zirconium oxychlorides are added to 250g deionizations
In water, while stirring be added dropwise mass fraction be 47% sodium hydroxide solution, regulation pH value be 11, filtering, take filter residue, spend from
Sub- water washing 4 times, dries, microwave drying 19min, finally obtains cupric oxide-oxidation with 1300 DEG C of roasting 3.5h in Muffle furnace
Zr catalyst.
The preparation of alkali lye：10g potassium hydroxide, the ammoniacal liquor that 25g mass fractions are 25% and 135g deionized waters are mixed, is stirred
Mix and uniformly obtain alkali lye.
The preparation of tri compound enzyme：68g acidic cellulases, 30g fructosidases and 22g protease are well mixed, are obtained
To tri compound enzyme.
Mix the preparation of alcoholic solution：It is 76% by ethanol water that 1550g mass fractions are 95%, 100g mass fractions
The normal propyl alcohol aqueous solution and the mixing of isopropanol water solution that 176g mass fractions are 85%, stir, obtain mixing alcoholic solution.
The method that the material that above-mentioned early stage is prepared is used for following extraction Arillus longan polysaccharides.
2. a kind of method for extracting Arillus longan polysaccharide, comprises the following steps：
(1) slurrying and activation process：The fresh arillus longans of 100g and 368g pure water are mixed, is poured into homogenizer and is made
Slurry, is subsequently adding 32g alkali lye, and 10h is activated at minus 9 DEG C, obtains activating solution；
(2) enzymolysis and concentration：The pH value for adjusting activating solution is 5, and 2g copper oxide-zirconium oxides are then added in filtrate
Catalyst and 7g tri compound enzymes, are digested under conditions of 260W microwaves, and the reaction temperature of controlled enzymatic hydrolysis is 70 DEG C
It is 46min with the reaction time, then the enzymolysis liquid obtained after enzymolysis is concentrated by ultrafiltration, 5-50KD molecules is retained with milipore filter
The enzymolysis liquid of amount, temperature be 48 DEG C, pressure be -0.09MPa under conditions of vacuum distillation to solid content be 92%, obtain
Concentrate；
(3) centrifugal treating：Concentrate and 590g mixed alcohol solution are mixed, after ultrasonically treated 30min, is received with centrifuge
Collection precipitate；
(4) spray drying treatment：Precipitate is washed with 88 DEG C of deionized water, rotation throwing disc rotating speed is put into after cooling is
23s is dried in the rotation type atomization drier of 22000r/min, Arillus longan polysaccharide is obtained.
Embodiment 6：
1. early-stage preparations：
The preparation of copper oxide-zirconium oxide catalyst：50g anhydrous cupric sulfates, 45g zirconium oxychlorides are added to 300g deionizations
In water, the sodium hydroxide solution that 25g mass fractions are 50% is added dropwise while stirring, regulation pH value is 12, and filtering takes filter residue, uses
Deionized water is washed 4 times, is dried, microwave drying 20min, finally obtains cupric oxide-oxygen with 1250 DEG C of roasting 4h in Muffle furnace
Change Zr catalyst.
The preparation of alkali lye：10g potassium hydroxide, the ammoniacal liquor that 30g mass fractions are 25% and 150g deionized waters are mixed, is stirred
Mix and uniformly obtain alkali lye.
The preparation of tri compound enzyme：70g acidic cellulases, 35g fructosidases and 30g protease are well mixed, are obtained
To tri compound enzyme.
Mix the preparation of alcoholic solution：It is 80% by ethanol water that 1600g mass fractions are 95%, 100g mass fractions
The normal propyl alcohol aqueous solution and the mixing of isopropanol water solution that 200g mass fractions are 90%, stir, obtain mixing alcoholic solution.
The method that the material that above-mentioned early stage is prepared is used for following extraction Arillus longan polysaccharides.
2. a kind of method for extracting Arillus longan polysaccharide, comprises the following steps：
(1) slurrying and activation process：The fresh arillus longans of 100g and 500g pure water are mixed, is poured into homogenizer and is made
Slurry, is subsequently adding 40g alkali lye, and 12h is activated at minus 10 DEG C, obtains activating solution；
(2) enzymolysis and concentration：The pH value for adjusting activating solution is 5.5, and 3g cupric oxide-oxidation is then added in filtrate
Zr catalyst and 8g tri compound enzymes, are digested under conditions of 500W microwaves, and the reaction temperature of controlled enzymatic hydrolysis is 75
DEG C and the reaction time be 60min, then the enzymolysis liquid obtained after enzymolysis is concentrated by ultrafiltration, retain 5-50KD point with milipore filter
Son amount enzymolysis liquid, temperature be 60 DEG C, pressure be -0.09MPa under conditions of vacuum distillation to solid content be 95%, obtain
To concentrate；
(3) centrifugal treating：Concentrate and 700g mixed alcohol solution are mixed, after ultrasonically treated 40min, is received with centrifuge
Collection precipitate；
(4) spray drying treatment：Precipitate is washed with 95 DEG C of deionized water, rotation throwing disc rotating speed is put into after cooling is
30s is dried in the rotation type atomization drier of 25000r/min, Arillus longan polysaccharide is obtained.
Comparative example 1：The method for extracting Arillus longan polysaccharide is substantially the same manner as Example 1, and difference is：Step does not have in (1)
Have using low temperature alkali lye activating technology；
Comparative example 2：The method for extracting Arillus longan polysaccharide is substantially the same manner as Example 1, and difference is：Step does not have in (2)
There is addition solid catalyst；
Comparative example 3：The method for extracting Arillus longan polysaccharide is substantially the same manner as Example 1, and difference is：Step does not have in (2)
Have using ultrafiltration concentration technology, but use Rotary Evaporators concentration.
Contrast test 1：
The Arillus longan polysaccharide in 100 fresh arillus longans, inspection are respectively extracted by the technical method of comparative example 1-3 and embodiment 1-6
The purity of Arillus longan polysaccharide yield and Arillus longan polysaccharide is surveyed, testing result is shown in Table 1.
Table 1：
The result of table 1 shows：The yield of Arillus longan polysaccharide is higher, illustrates that the production efficiency of the technical method is higher, longan
Meat polysaccharide yield is arranged as from high to low：Embodiment 4>Embodiment 3=embodiments 6>Embodiment 2>Embodiment 1>Embodiment 5>It is right
Ratio 2>Comparative example 3>Comparative example 1；
The purity of Arillus longan polysaccharide is higher, illustrates that the production efficiency of the technical method is higher, the purity of Arillus longan polysaccharide from
It is high to Low to be arranged as：Embodiment 4>Embodiment 5>Embodiment 2>Embodiment 6>Embodiment 3>Embodiment 1>Comparative example 3>Comparative example 3>
Comparative example 1.
Described above is directed to the detailed description of preferably possible embodiments of the invention, but embodiment is not limited to this hair
Bright patent claim, the equal change completed under the technical spirit suggested by all present invention or modification change, all should belong to
Cover the scope of the claims in the present invention.
Claims (8)
1. it is a kind of extract Arillus longan polysaccharide method, it is characterised in that：Comprise the following steps：
(1) slurrying and activation process：Fresh arillus longan and pure water are mixed, slurrying in homogenizer is poured into, alkali is subsequently adding
Liquid, 10-12h is activated at negative 4-10 DEG C, obtains activating solution；
(2) enzymolysis and concentration：Adjust activating solution pH value, then in filtrate add copper oxide-zirconium oxide catalyst and
Tri compound enzyme, is digested under conditions of 200-500W microwaves, the reaction temperature of controlled enzymatic hydrolysis and reaction time, is connect
The enzymolysis liquid that will be obtained after enzymolysis to be concentrated by ultrafiltration, obtain concentrate；
(3) centrifugal treating：Concentrate and mixed alcohol solution are mixed, after ultrasonically treated 25-40min, is analysed with centrifugal collection
Go out thing；
(4) spray drying treatment：Precipitate is washed with 85-95 DEG C of deionized water, rotation throwing disc rotating speed is put into after cooling is
10-30s is dried in the rotation type atomization drier of 15000-25000r/min, Arillus longan polysaccharide is obtained.
2. it is according to claim 1 it is a kind of extract Arillus longan polysaccharide method, it is characterised in that：Oxygen described in step (2)
Change copper-zirconia catalyst to be prepared by following methods：Count by weight, by 35-50 parts of anhydrous cupric sulfate, 30-45 parts of oxygen chlorine
Change zirconium to be added in 200-300 parts of deionized water, it is the sodium hydroxide solution of 40-50% that mass fraction is added dropwise while stirring, is adjusted
Section pH value is 9-12, and filtering takes filter residue, is washed with deionized 2-4 times, dries, microwave drying 10-20min, finally in Muffle
With 1100-1300 DEG C of roasting 2-4h in stove, copper oxide-zirconium oxide catalyst is obtained.
3. it is according to claim 1 it is a kind of extract Arillus longan polysaccharide method, it is characterised in that：Alkali described in step (1)
Liquid is by weight 1：1-3：The potassium hydroxide of 10-15, mass fraction are that 25% ammoniacal liquor and deionized water are mixed.
4. it is according to claim 1 it is a kind of extract Arillus longan polysaccharide method, it is characterised in that：Described in step (2) three
First complex enzyme is by weight 50-70：20-35：The acidic cellulase of 15-30, fructosidase and protease are mixed.
5. it is according to claim 1 it is a kind of extract Arillus longan polysaccharide method, it is characterised in that：PH described in step (2)
It is 4.5-5.5 to be worth, and described reaction temperature is 50-75 DEG C, and the described reaction time is 40-60min.
6. it is according to claim 1 it is a kind of extract Arillus longan polysaccharide method, it is characterised in that：It is super described in step (2)
Filter concentration is the enzymolysis liquid that 5-50KD molecular weight is retained using milipore filter, is 40-60 DEG C in temperature, and pressure is the bar of -0.09MPa
Vacuum distillation to solid content is 80-95% under part.
7. it is according to claim 1 it is a kind of extract Arillus longan polysaccharide method, it is characterised in that：It is mixed described in step (3)
It is by weight 10-16 to close alcoholic solution：1：The mass fraction of 1-2 be 95% ethanol water, mass fraction be 70-80%
The normal propyl alcohol aqueous solution and the isopropanol water solution that mass fraction is 70-90% are mixed.
8. the method for a kind of extraction Arillus longan polysaccharide according to claim 1-7 any one, it is characterised in that：Described
Fresh arillus longan, pure water, alkali lye, copper oxide-zirconium oxide catalyst, tri compound enzyme and mixing alcoholic solution weight ratio be
100：250-500：20-40：1-3：5-8：500-700.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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| CN108841560A (en) * | 2018-06-29 | 2018-11-20 | 广西壮族自治区农业科学院农产品加工研究所 | A kind of fragrance high-fidelity type passiflora edulis vinegar and its brewing method |
| CN108841477A (en) * | 2018-06-29 | 2018-11-20 | 广西壮族自治区农业科学院农产品加工研究所 | A method of western melon wine is made using micro-aerobe fermentation technology |
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| CN116854835A (en) * | 2023-04-04 | 2023-10-10 | 华南农业大学 | High-immunocompetence acetylated longan water-soluble glucan, and preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108611249A (en) * | 2018-06-29 | 2018-10-02 | 广西壮族自治区农业科学院农产品加工研究所 | A method of cucumber vinegar is made using micro-aerobe fermentation technology |
| CN108841560A (en) * | 2018-06-29 | 2018-11-20 | 广西壮族自治区农业科学院农产品加工研究所 | A kind of fragrance high-fidelity type passiflora edulis vinegar and its brewing method |
| CN108841477A (en) * | 2018-06-29 | 2018-11-20 | 广西壮族自治区农业科学院农产品加工研究所 | A method of western melon wine is made using micro-aerobe fermentation technology |
| CN108913420A (en) * | 2018-06-29 | 2018-11-30 | 广西壮族自治区农业科学院农产品加工研究所 | A kind of fragrance high-fidelity type Fragrant fruit wine and its brewing method |
| CN108611249B (en) * | 2018-06-29 | 2021-09-14 | 广西壮族自治区农业科学院农产品加工研究所 | Method for brewing cucumber vinegar by adopting micro-aerobic fermentation technology |
| CN108841477B (en) * | 2018-06-29 | 2021-10-01 | 广西壮族自治区农业科学院农产品加工研究所 | Method for brewing watermelon and muskmelon wine by adopting micro-aerobic fermentation technology |
| CN108841560B (en) * | 2018-06-29 | 2021-10-26 | 广西壮族自治区农业科学院农产品加工研究所 | Fragrant high-fidelity passion fruit vinegar and brewing method thereof |
| CN108913420B (en) * | 2018-06-29 | 2021-10-26 | 广西壮族自治区农业科学院农产品加工研究所 | Aroma high-fidelity passion fruit wine and brewing method thereof |
| CN116854835A (en) * | 2023-04-04 | 2023-10-10 | 华南农业大学 | High-immunocompetence acetylated longan water-soluble glucan, and preparation method and application thereof |
| CN116854835B (en) * | 2023-04-04 | 2024-02-02 | 华南农业大学 | High-immunocompetence acetylated longan water-soluble glucan, and preparation method and application thereof |
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