CN106748190A - One kind fermentation Optimization of Low Value Fish water-soluble fertilizer and preparation method thereof - Google Patents
One kind fermentation Optimization of Low Value Fish water-soluble fertilizer and preparation method thereof Download PDFInfo
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- CN106748190A CN106748190A CN201611234780.1A CN201611234780A CN106748190A CN 106748190 A CN106748190 A CN 106748190A CN 201611234780 A CN201611234780 A CN 201611234780A CN 106748190 A CN106748190 A CN 106748190A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F1/00—Fertilisers made from animal corpses, or parts thereof
- C05F1/002—Fertilisers made from animal corpses, or parts thereof from fish or from fish-wastes
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Pest Control & Pesticides (AREA)
- Environmental & Geological Engineering (AREA)
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Abstract
The invention belongs to technical field of fertilizers, and in particular to one kind fermentation Optimization of Low Value Fish water-soluble fertilizer and preparation method thereof.The preparation method has includes that marine low-value fish is chopped into fragment by S1, is subsequently added into water, disodium ethylene diamine tetraacetate and APG, and to 3~5, agitated rear standing is filtered, the fragment after being purified regulation system pH value；Above-mentioned fragment is crushed and is made minced fillet by S2, is transferred in fermentation tank, is subsequently added into molasses and bacillus, lactic acid bacteria, saccharomycete, is fermented at room temperature, is stirred continuously during the fermentation, and the molten slurry of fermented fish is obtained；S3 stirs toward preservative is added in the molten slurry of the fermented fish, and fermentation Optimization of Low Value Fish water-soluble fertilizer is obtained.The salinity of the fertilizer is less than 2%, and the heavy metal such as arsenic sum is less than 20ppm, and rich in active peptide, immune polysaccharide, amino acid and unrighted acid etc., ground temperature can be improved after being manured into soil, and increases root system activity, strengthens crop cold resistance, and then promote plant growth.
Description
Technical field
The invention belongs to technical field of fertilizers, and in particular to one kind fermentation Optimization of Low Value Fish water-soluble fertilizer and preparation method thereof.
Background technology
Current agricultural crops are to rely on chemical fertilizer, chemical agent to improve yield mostly, and long-term use destroys nature in soil
The chemical balance that microbiologic population is constituted, makes the physics of soil and electrochemical conditions uneven, the natural fertility deep fades of soil,
So as to cause annual needs using more chemical fertilizer to maintain high yield, thus cost is increased, gone down for a long time, or even can make
Into the barren of soil with fall into disuse.
Optimization of Low Value Fish refers to that in the market value ratio is relatively low, and single yield is not high, it is impossible to used as the small fish that independent species is sold.
China's marine fishery resources enriches, and is one of maximum country of output of aquatic products in the world, wherein, in the aquatic products of marine fishing
The yield accounting of low-value small fish is larger, typically constitutes from the 28~59% of fisher.Low-value small fish is individual smaller, typically has more
Tiny spur, partially due to fat content is high to cause to have a poor flavour, makes low-value small fish be unsuitable for directly food in general
With, therefore it is more for producing the low value product such as breeding feed, fish meal, or lost directly as discarded object due to rotting
Abandon, cause the waste of ample resources, also result in the pollution of environment.The protein content of low-value small fish is higher, general 15~
18% or so, it is a preferable albumen data source, high molecular weight protein is degraded to oligomeric active peptide after fermentation, can be used as fertilizer
Use, improve the added value of low value fish processing, obtain good economic benefit and social benefit.
At present, fertilizer is produced mainly using the steps such as simple boiling, fermentation, enzymolysis, separation, such as China using Optimization of Low Value Fish
A kind of preparation method of Fish protein amino acid water-soluble fertilizer disclosed in patent application CN 104557339A, it crushes Optimization of Low Value Fish
Into after fish slurry, add saccharomycete, lactic acid bacteria and protease to be fermented, water-soluble fertilizer is obtained.But so obtained Optimization of Low Value Fish
Salinity, content of beary metal in fertilizer product, especially marine low-value guano product is higher, if this kind of fertilizer of long-term use
May result in the heavy metal pollution of soil.And this kind of fertilizer oil content is higher, product is guaranteed the quality difficulty, limits its popularization
Using.It is that raw material produces water-soluble Fish protein organic fertilizer that Chinese patent application CN 104892037A disclose one kind with fish
Method, it uses acidulous cation resin, weak anion resin post, while being chelated respectively in zwitterion resin
The mode of property resin is eluted to enzymolysis liquid, and to remove salinity therein and heavy metal, but this sample loading mode is relatively time-consuming, unfavorable
In large-scale production.
The content of the invention
In order to solve problems of the prior art (salinity, content of beary metal such as in marine low-value guano product
It is higher, difficulty of guaranteeing the quality etc.), the invention provides a kind of preparation method of the Optimization of Low Value Fish water-soluble fertilizer that ferments, the method can effectively drop
Salinity and heavy metal ion content in low marine low-value fish, and obtained fertilizer product stable system, long shelf-life.
A kind of preparation method of fermentation Optimization of Low Value Fish water-soluble fertilizer that the present invention is provided, specifically includes following steps：
S1, the fragment that marine low-value fish is chopped into 0.5~1cm, are subsequently added into 3~5 times of water of amount of fragment weight, then
Disodium ethylene diamine tetraacetate and APG are added, it is quiet after 20~30min of stirring using citric acid regulation system pH value to 3~5
2~3h is put, is filtered, the fragment after being purified；
S2, by after the purification fragment crush be made minced fillet, be transferred in fermentation tank, be subsequently added into molasses and gemma bar
Bacterium, lactic acid bacteria, saccharomycete, ferment 5~7 days at room temperature, are stirred continuously during the fermentation, and it is molten that fermentation ends are obtained fermented fish
Slurry；
S3, toward the preservative that its weight 0.2~1.0% is added in the molten slurry of the fermented fish, stir, fermentation is obtained low
Value fish water-soluble fertilizer.
Further, the addition of the disodium ethylene diamine tetraacetate is the 4~6% of the fragment weight.
Further, the addition of the APG is the 1~3% of the fragment weight, and APG is specially
Carbon chain lengths are the APG of C8~C10.
Further, the percentage by weight shared by the minced fillet, molasses, bacillus, lactic acid bacteria and saccharomycete is respectively
30%th, 55%, 6%, 3% and 6%.
Further, the bacillus includes bacillus subtilis and Methylotrophic bacillus, the withered grass bud
The weight ratio of spore bacillus and Methylotrophic bacillus is 1:1.
Further, the lactic acid bacteria includes lactobacillus fermenti and Lactobacillus brevis, the lactobacillus fermenti and Lactobacillus brevis
Weight ratio be 2:1.
Further, the preservative is the mixture of sodium dehydroacetate and curcumin, the sodium dehydroacetate and turmeric
The weight ratio of element is (3~5):1.
In the inventive solutions, the marine low-value fish is specially anchovy, small yellow croaker, small flatfish etc., this
Class fish lives in coastal waters, and salinity and contaminated degree are higher, therefore, marine low-value fish is chopped into fragment by the present invention first, with
Just desalination and heavy metal treatment are carried out to it；Disodium ethylene diamine tetraacetate is a kind of good chelating agent, can effectively be chelated hard
Many kinds of metal ions in water, such as calcium, magnesium, iron, lead, copper, but inventor has found to be applied to chelating marine low-value fish
Heavy metal ion in meat, it does not work substantially, it may be possible to because heavy metal ion is combined with the flesh of fish, is difficult to be chelated and removes
Go, need to solve the problems, such as heavy metal ion dissolution in the flesh of fish for this.APG is a kind of surfactant, and it is water-soluble
It is good, and after inventor is surprised to find that to use a small amount of APG, because of its surface-active, serves absorption and change osmotic pressure
Effect so that heavy metal ion in the flesh of fish can effectively dissolution, while also accelerating the dissolution of salinity, substantially reduced so as to reach
The purpose of heavy metal ion and salt content, and when using citric acid regulation system pH value to 3~5, action effect is optimal.
After desalination and heavy metal treatment are carried out to marine low-value fish, fish fragment is crushed and is made minced fillet, add molasses and bacillus, breast
Sour bacterium etc. is fermented, and it is the fermentation Optimization of Low Value Fish water-soluble fertilizer that liquid is obtained that preservative is added after fermentation ends.Anti-corrosion of the present invention
Agent is by sodium dehydroacetate and curcumin (chemical name：Double (4- hydroxy 3-methoxybenzenes the base) -1,6- heptadiene of (E, E) -1,7- -
3,5- diketone, No. CAS：458-37-7) mix, can effectively extend the shelf-life of fertilizer of the present invention, it is pushed away on a large scale
Wide application.
In addition, the present invention is also claimed the low value that fermented as obtained in the preparation method of above-mentioned fermentation Optimization of Low Value Fish water-soluble fertilizer
Fish water-soluble fertilizer.The salinity of the fertilizer is less than 2%, and arsenic, chromium, cadmium, five heavy metal ion content sums of lead and mercury are less than
20ppm, and rich in active peptide, immune polysaccharide, amino acid and unrighted acid etc., ground temperature can be improved after being manured into soil, increase
Plus root system activity, strengthen crop cold resistance, and then promote plant growth.
Therefore, compared with prior art, advantage of the invention is that：
(1) preparation method of present invention fermentation Optimization of Low Value Fish water-soluble fertilizer is simple, and process stabilizing, condition is controllable, production cost
It is low, can industrialized production；And salinity and heavy metal ion content in marine low-value fish can be effectively reduced, extend guaranteeing the quality for fertilizer
Phase, be conducive to large-scale promotion application.
(2) present invention fermentation Optimization of Low Value Fish water-soluble fertilizer salinity be less than 2%, arsenic, chromium, cadmium, five heavy metals of lead and mercury from
Sub- content sum is less than 20ppm, and fertilizer of the present invention rich in active peptide, immune polysaccharide, amino acid and unrighted acid etc.,
Ground temperature can be improved after being manured into soil, increases root system activity, promote root growth and development；Improve anti-adversity ability, especially cold-resistant energy
Power；Promote plant growth, increase crop yield, improve crop quality.
Specific embodiment
Hereinafter, the present invention will be further described in conjunction with specific embodiments.
The embodiment of the present invention 1~3 and comparative example 1~5 are used and processed with a collection of marine low-value fish.The present invention is implemented
The raw material used in example is conventional commercial commodity.
Embodiment 1, the present invention fermentation Optimization of Low Value Fish water-soluble fertilizer and its preparation
S1, the fragment that marine low-value fish is chopped into 0.5~1cm, take 5 tons of fragments, are subsequently added into 15 tons of water, are subsequently adding
0.2 ton of disodium ethylene diamine tetraacetate and 0.05 ton of carbon chain lengths are the APG of C8~C10, use citric acid regulation system pH
Value stands 2h to 3 after stirring 20min, filters, the fragment after being purified；
S2, by after the purification fragment crush be made minced fillet, take 3 tons of minced fillets, be transferred in fermentation tank, be subsequently added into
5.5 tons of molasses and 0.6 ton of bacillus, 0.3 ton of lactic acid bacteria, 0.6 ton of saccharomycete, ferment 5 days, during the fermentation at room temperature
It is stirred continuously, fermentation ends are obtained the molten slurry of fermented fish；
S3, toward the preservative that its weight 0.2% is added in the molten slurry of the fermented fish, stir, fermentation Optimization of Low Value Fish is obtained
Water-soluble fertilizer.
The bacillus includes bacillus subtilis and Methylotrophic bacillus, the bacillus subtilis and first
The weight ratio of base auxotype bacillus is 1:1.
The lactic acid bacteria includes lactobacillus fermenti and Lactobacillus brevis, and the weight ratio of the lactobacillus fermenti and Lactobacillus brevis is
2:1。
The preservative is the weight ratio of the mixture of sodium dehydroacetate and curcumin, the sodium dehydroacetate and curcumin
It is 3:1.
Embodiment 2, the present invention fermentation Optimization of Low Value Fish water-soluble fertilizer and its preparation
S1, the fragment that marine low-value fish is chopped into 0.5~1cm, take 5 tons of fragments, are subsequently added into 25 tons of water, are subsequently adding
0.3 ton of disodium ethylene diamine tetraacetate and 0.15 ton of carbon chain lengths are the APG of C8~C10, use citric acid regulation system pH
Value stands 3h to 5 after stirring 30min, filters, the fragment after being purified；
S2, by after the purification fragment crush be made minced fillet, take 3 tons of minced fillets, be transferred in fermentation tank, be subsequently added into
5.5 tons of molasses and 0.6 ton of bacillus, 0.3 ton of lactic acid bacteria, 0.6 ton of saccharomycete, ferment 7 days, during the fermentation at room temperature
It is stirred continuously, fermentation ends are obtained the molten slurry of fermented fish；
S3, toward the preservative that its weight 1.0% is added in the molten slurry of the fermented fish, stir, fermentation Optimization of Low Value Fish is obtained
Water-soluble fertilizer.
The bacillus includes bacillus subtilis and Methylotrophic bacillus, the bacillus subtilis and first
The weight ratio of base auxotype bacillus is 1:1.
The lactic acid bacteria includes lactobacillus fermenti and Lactobacillus brevis, and the weight ratio of the lactobacillus fermenti and Lactobacillus brevis is
2:1。
The preservative is the weight ratio of the mixture of sodium dehydroacetate and curcumin, the sodium dehydroacetate and curcumin
It is 5:1.
Embodiment 3, the present invention fermentation Optimization of Low Value Fish water-soluble fertilizer and its preparation
S1, the fragment that marine low-value fish is chopped into 0.5~1cm, take 5 tons of fragments, are subsequently added into 20 tons of water, are subsequently adding
0.25 ton of disodium ethylene diamine tetraacetate and 0.1 ton of carbon chain lengths are the APG of C8~C10, use citric acid regulation system pH
Value stands 2h to 4 after stirring 30min, filters, the fragment after being purified；
S2, by after the purification fragment crush be made minced fillet, take 3 tons of minced fillets, be transferred in fermentation tank, be subsequently added into
5.5 tons of molasses and 0.6 ton of bacillus, 0.3 ton of lactic acid bacteria, 0.6 ton of saccharomycete, ferment 6 days, during the fermentation at room temperature
It is stirred continuously, fermentation ends are obtained the molten slurry of fermented fish；
S3, toward the preservative that its weight 0.5% is added in the molten slurry of the fermented fish, stir, fermentation Optimization of Low Value Fish is obtained
Water-soluble fertilizer.
The bacillus includes bacillus subtilis and Methylotrophic bacillus, the bacillus subtilis and first
The weight ratio of base auxotype bacillus is 1:1.
The lactic acid bacteria includes lactobacillus fermenti and Lactobacillus brevis, and the weight ratio of the lactobacillus fermenti and Lactobacillus brevis is
2:1。
The preservative is the weight ratio of the mixture of sodium dehydroacetate and curcumin, the sodium dehydroacetate and curcumin
It is 4:1.
Comparative example 1
Compared with Example 3, this comparative example is differed only in：Do not use APG.
Comparative example 2
Compared with Example 3, this comparative example is differed only in：The addition of the APG is the fragment weight
5%, i.e., 0.25 ton.
Comparative example 3
Compared with Example 3, this comparative example is differed only in：Use citric acid regulation system pH value to 2.
Comparative example 4
Compared with Example 3, this comparative example is differed only in：The preservative is sodium dehydroacetate.
Comparative example 5
Compared with Example 3, this comparative example is differed only in：The preservative is curcumin.
Test example one, removing salt and heavy metal ion effect assessment
To the salt content of marine low-value fish fragment after step S1 before processings in embodiment 1~3 and the method for comparative example 1~3 and
Heavy metal ion content (arsenic, chromium, cadmium, five heavy metal ion sums of lead and mercury) is detected, as a result such as following table：
As seen from the above table：
(1) preparation method of the fermentation of the embodiment of the present invention 1~3 Optimization of Low Value Fish water-soluble fertilizer can make the salinity and a huge sum of money in the flesh of fish
The category effective dissolution of ion, reaches the purpose for substantially reducing salinity and heavy metal ion content so that salinity is less than in fertilizer product
2%, arsenic, chromium, cadmium, five heavy metal ion content sums of lead and mercury are less than 20ppm, and salinity and heavy metal ion can be avoided to bring
Soil pollution.
(2) comparative example 1 does not use APG, the salt content and heavy metal ion of marine low-value fish fragment after its before processing
Content is only slightly reduced；Comparative example 2 using addition higher APG, as a result its treatment after fish fragment salt content and
Heavy metal ion content is higher than embodiment, illustrates that the not more removal effects of addition of APG are better, its
Consumption will could obtain optimal action effect within the specific limits；The regulation system pH value of comparative example 3 to 2, as a result after its treatment
Fish fragment salt content and heavy metal ion content it is also higher than embodiment, illustrate pH value in the method for the invention
Size can influence to remove the effect of salt and heavy metal ion.
Test example two, shelf-life is evaluated
To embodiment 1~3 and the shelf-life of fertilizer obtained in comparative example 4~5 is investigated (at ambient temperature), as a result
Such as following table：
As seen from the above table：
(1) long shelf-life of the fermentation of the embodiment of the present invention 1~3 Optimization of Low Value Fish water-soluble fertilizer is up to 36 months, fertilizer during this period
Remain brown fishlike smell liquid, without going mouldy, phenomenon of becoming sour, pH value is more constant, it is higher to solve low value guano oil content,
The problem of difficulty of guaranteeing the quality, is conducive to the large-scale promotion application of fertilizer of the present invention；
(2) compared with Example 3, the preservative of the application of comparative example 4 is sodium dehydroacetate, and the fertilizer was checked at 18th month
When gone bad, there is white mould on its top layer, and with acid smell, pH value (1:250) 2.1 are down to；And the application of comparative example 5 is anti-
Rotten agent is curcumin, and the fertilizer has gone bad when checking within 12nd month, and there is white mould on its top layer, and with acid smell, pH
Value (1:250) 1.4 are down to, sodium dehydroacetate are reflected and curcumin is had certain synergy, both are multiple by a certain percentage
The shelf-life of fertilizer of the present invention can be extended with use.
The above, is only presently preferred embodiments of the present invention, and any formal limitation is not made to the present invention, this
Art personnel make a little simple modification, equivalent variations or modification using the technology contents of the disclosure above, all fall within this hair
In bright protection domain.
Claims (8)
1. it is a kind of ferment Optimization of Low Value Fish water-soluble fertilizer preparation method, it is characterised in that comprise the following steps：
S1, the fragment that marine low-value fish is chopped into 0.5~1cm, are subsequently added into 3~5 times of water of amount of fragment weight, are subsequently adding
Disodium ethylene diamine tetraacetate and APG, using citric acid regulation system pH value to 3~5,2 are stood after 20~30min of stirring
~3h, filtering, the fragment after being purified；
S2, the fragment after the purification crushed be made minced fillet, be transferred in fermentation tank, be subsequently added into molasses and bacillus,
Lactic acid bacteria, saccharomycete, ferment 5~7 days at room temperature, are stirred continuously during the fermentation, and fermentation ends are obtained the molten slurry of fermented fish；
S3, toward the preservative that its weight 0.2~1.0% is added in the molten slurry of the fermented fish, stir, fermentation Optimization of Low Value Fish is obtained
Water-soluble fertilizer.
2. the preparation method of fermentation Optimization of Low Value Fish water-soluble fertilizer as claimed in claim 1, it is characterised in that the ethylenediamine tetrem
The addition of acid disodium is the 4~6% of the fragment weight.
3. the preparation method of fermentation Optimization of Low Value Fish water-soluble fertilizer as claimed in claim 1, it is characterised in that the APG
Addition is the 1~3% of the fragment weight.
4. the preparation method of fermentation Optimization of Low Value Fish water-soluble fertilizer as claimed in claim 1, it is characterised in that the minced fillet, molasses,
Percentage by weight shared by bacillus, lactic acid bacteria and saccharomycete is respectively 30%, 55%, 6%, 3% and 6%.
5. the preparation method of fermentation Optimization of Low Value Fish water-soluble fertilizer as claimed in claim 1, it is characterised in that the bacillus bag
Include bacillus subtilis and Methylotrophic bacillus.
6. the preparation method of fermentation Optimization of Low Value Fish water-soluble fertilizer as claimed in claim 1, it is characterised in that the lactic acid bacteria includes
Lactobacillus fermenti and Lactobacillus brevis.
7. the preparation method of fermentation Optimization of Low Value Fish water-soluble fertilizer as claimed in claim 1, it is characterised in that the preservative is de-
The weight ratio of the mixture of acetate hydrogen sodium and curcumin, the sodium dehydroacetate and curcumin is (3~5):1.
8. the fermentation Optimization of Low Value Fish obtained in the preparation method of Optimization of Low Value Fish water-soluble fertilizer that fermented as described in claim 1~7 is any is water-soluble
Fertilizer.
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| CN107348232A (en) * | 2017-07-19 | 2017-11-17 | 合肥浦邦农业科技有限公司 | A kind of preparation method of new type water-solubility guano |
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| CN109053298A (en) * | 2018-10-18 | 2018-12-21 | 舟山丰宇三立海洋生物肥料有限公司 | A kind of dedicated fertilizer and preparation method thereof improving fruits and vegetables cold tolerance |
| CN111072410A (en) * | 2020-01-02 | 2020-04-28 | 杭州正肽生物科技有限公司 | Liquid fertilizer containing small peptides and application thereof |
| CN112322303A (en) * | 2020-11-03 | 2021-02-05 | 宁波吉丰生物科技发展有限公司 | Organic biological cadmium-reducing soil conditioner containing fish protein and preparation method thereof |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111072410A (en) * | 2020-01-02 | 2020-04-28 | 杭州正肽生物科技有限公司 | Liquid fertilizer containing small peptides and application thereof |
| CN112322303A (en) * | 2020-11-03 | 2021-02-05 | 宁波吉丰生物科技发展有限公司 | Organic biological cadmium-reducing soil conditioner containing fish protein and preparation method thereof |
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| Publication number | Publication date |
|---|---|
| CN106748190B (en) | 2018-03-20 |
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