CN105367307A - Selenium-rich biological agent for improving activity of microbes in soil and preparation method of selenium-rich biological agent - Google Patents

Selenium-rich biological agent for improving activity of microbes in soil and preparation method of selenium-rich biological agent Download PDF

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CN105367307A
CN105367307A CN201510989336.XA CN201510989336A CN105367307A CN 105367307 A CN105367307 A CN 105367307A CN 201510989336 A CN201510989336 A CN 201510989336A CN 105367307 A CN105367307 A CN 105367307A
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selenium
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enriched bio
bio preparation
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李晓
王曙光
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Shandong University
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Shandong University
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    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05B—PHOSPHATIC FERTILISERS
    • C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80—Soil conditioners

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  • Life Sciences & Earth Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Plant Pathology (AREA)
  • Soil Sciences (AREA)
  • Fertilizers (AREA)

Abstract

The invention relates to a selenium-rich biological agent for improving the activity of microbes in soil. The selenium-rich biological agent comprises the following raw materials: 8-15 parts of sodium selenite, 5-15 parts of compound trace elements, 8-20 parts of monopotassium phosphate, 10-25 parts of urea, 8-20 parts of peptone, 4-10 parts of yeast powder, 8-20 parts of NaCl, 8-15 parts of NaOH, 4-10 parts of NaNO3, 10-25 parts of NaCl, 4-10 parts of NH4Cl, 8-20 parts of K2HPO4, 4-10 parts of a beef extract, 10-25 parts of glucose, 10-25 parts of starch, 8-20 parts of calcium carbonate, 10-25 parts of amino acid, 2-6 parts of potassium nitrate, 2-6 parts of ferrous sulfate, 10-25 parts of magnesium sulfate, 25-40 parts of trisodium citrate, 10-25 parts of enzymes and 800-896 parts of excess activated sludge. The selenium-rich biological agent adopts excess activated sludge produced by treatment of domestic sewage, so that not only is the fermentation speed of the selenium-rich biological agent increased, but also the particle diameters of selenium nano-particles are more uniform, and absorption of the selenium-rich biological agent by plants is benefitted.

Description

A kind of Se-enriched bio preparation and preparation method improving Soil Microorganism activity
Technical field
The present invention relates to a kind of selenium-rich nutritive type microbial preparation, specifically a kind of Se-enriched bio preparation and preparation method improving Soil Microorganism activity, belongs to technical field of biological fertilizer.
Background technology
China is a Ge Que selenium big country.GeneraI investigation proves, seriously in the Soils In The Region of China more than 70% lacks selenium, and in the grain of China more than 95% and livestock and poultry meat, selenium content is lower than 50 μ g/kg, belongs to Pin Xi big country.Every day, selenium intake only had 39 μ about g per capita in China, and not enough grownup triumph needs 80% of minimum 50 μ g/ days, larger apart from healthy requirement 100 ~ 200 μ g/ days differences; Farther apart from the 800 μ g that reach the standard grade safely.In China, except minority area, the population of 90% needs to adopt biotechnology supplement selenium.Not only people wants supplement selenium, and livestock and poultry also want supplement selenium.
China is large agricultural country, and chemical fertilizer plays an important role in China's agriculture production.But, along with the consumption of unit surface chemical fertilizer is more and more higher, there is the sight that serious soil " is more fed thinner ".What accompany with it is not only the degeneration of soil, the increase of applications of pesticide amount and the decline of quality of agricultural product, also has the underground water of nearly 40% because nitrate content is too high can not directly drink.Therefore, development environment friendly fertilizer is the development strategy of China.Selenium-enriched microbe bacterial manure because of its there is soil function improvement, increasing crop yield, quality of agricultural product improve, fertility is lasting and advantages of environment protection, and are subject to extensive concern.The microbial fertilizer history of existing more than 100 year so far, oneself in Asia, America, Europe And Africa etc. in the world more than 60 country apply.American and Britain, method, Deng developed country, increase the Innovation Input of microbial-bacterial fertilizer, obtain kind abundanter in recent years, the functionalization microbial-bacterial fertilizer that performance is more excellent, instead of the fertilizer application amount of about 40%.China also pays much attention to the development & application of green fertilizer, and microbial fertilizer industry achieves certain development, manufacturing enterprise more than 800, annual production nearly ten million ton, annual value of production crosses 10,000,000,000 yuan, but with the same period chemical fertilizer output compare with consumption, only account for less than 5%.But along with the raising that people require quality of the life, the appealing of developing green pollution-free food is more and more higher, this social demand will cause the market demand of microbial fertilizer increasing.
Selenium is one of necessary nutritional trace element of human body.Human or animal lacks or the selenium of excess intake, then can cause some disease, the sickness rate such as Keshan disease, Kaschin-Beck disease, myocardosis, cancer as scarce selenium area is very high.Research and practice proves, selenium element plays inherent keying action to HUMAN HEALTH, and it is glutathione peroxidase, thioredoxin reductase and subtract key component---the activator of the endogenous antioxidant such as iodine enzyme.It for adjustment human immune system, resist disease, delay senility, to prevent and the various diseases such as auxiliary for treating cancer plays an important role, be particularly that other antioxidants are incomparable and alternative in removing lipid free radical.
Selenium be just be found in recent decades very important, indispensable to HUMAN HEALTH, have the overall situation effect endogenous strong anti-oxidation material and free-radical scavengers.World Health Organization WHO announces: selenium is that life hits necessary trace element, selenium is respectfully called " kindling material of life " by domestic and international the world of medicine, enjoys the good reputation such as " long-lived element ", " anticancer king ", " heart patron saint ", " natural toxinicide ".Selenium has been known as by scientific and technological circle and " has contributed to the torment that the mankind exempt various slight illness, as unhealthy emotion, pancreatic disease, diabetes, reproductive system disease, virus infection and cancer etc." there is report, the people that in blood, selenium content is minimum dies from 3 times that cardiopathic probability is content height person.Selenium can make the immunity system of human body again rejuvenate.Selenium can discharge heavy metal of body toxin, and selenium is that seleno-protein runs into rapid combinations of poisonous metal ash such as lead, mercury, cadmium, stone roller and becomes harmless compounds and excrete with the form of selenium propylhomoserin composition seleno-protein in vivo.Selenium is the conditioning agent of iodine in body, and it plays very important regulating effect to iodine in thyroid hormone metabolism process.What be combined with selenium also has a kind of very important antioxidase---thioredoxin reductase, has a lot of biological function, affects the synthesis of nucleic acid.Selenium prophylaxis is infertile, and particularly prevent the symptoms such as the male sex dead essence, oligospermia, many data show, today infertility and scarce selenium have direct relation.Man and animal takes in ensureing by way of the food mainly by containing selenium of selenium, and therefore, the preparation of microbiological medicine containing rich selenium and the plantation of rich selenium product are very important.
Can the selenium that absorbs by human body be divided into elemental selenium, inorganic selenium and organoselenium three kinds.The feature of selenium is that between biological activity and toxicity, scope is extremely narrow, and for this reason, various countries scientist have studied many selenium compounds, attempts to find the selenium form that activity is high, toxicity is low.Along with the maturation of nanotechnology, the nanotechnology that scientist is advanced, zeroth order selenium (one of elemental selenium) is made nano particle selenium--nanometer selenium.Nanometer selenium, compared with general zeroth order selenium, there occurs the sudden change of biological property.Concrete following characteristics: 1) there is oxidation-resistance; 2) due to highly active characteristic, thus the hazardness of human body is greatly reduced, the nutrient contg that low dosage selenium is equivalent to high dosage in the past can be supplemented, thus avoid the side effect brought to human body.So nanometer selenium makes the structure of matter of selenium become the zeroth order material of nanomorphic by the compound of original molecular state, not only make human body fully absorb selenium, reduce its toxicity, but also possess good biological activity.
Mud is the byproduct after sewage disposal, usually account for the 0.3%-0.5% (volume) of sewage quantity or be about the 1%-2% (quality) of sewage load, the mud that general municipal sewage plant produces is about about 0.5% of the volume of process water, so a large amount of mud, if without reasonably treatment and use, a serious problem of environmental pollution will be become.But the present situation of China is that most mud is unprocessed at present, be simply discarded, landfill, or misused in farmland by peasant, produce secondary pollution to environment, therefore finding effective specific resistance to filtration method will have huge economy, Environment intension.
Municipal sludge is the by product of municipal sewage plant's Wastewater Treated by Activated Sludge Process waste water, and it is a kind of extremely complicated heterogeneous body be made up of organic substance relic, microorganism, inorganic particle, organic-inorganic colloid etc.Therefore mud is generally the concentrated thing of asking being in liquid and circle.Concentration of suspension in mud generally 1% one 10%, is usually called mud lower than this concentration.The biological treatment mud that the mixing sludge profit trade effluent that sanitary sewage disposal produces produces is typical organic sludge, its characteristic is organic content high (60%-80%), particle thin (0.02-0.2mm), density little (1.002-1.0069g/cm3), in colloidal structure, coagulated sludge, containing more organism in mud, according to investigations, organic content in China's mud is about 50%-70%, but carbohydrate content is higher, belong to Hi CHO lower fat type.In mud containing abundant N, P, K (in China's mud TN, TP, TK average content be respectively 3.03%, 1.52% and O.69%) etc. nutritive element and plant necessary various micro-ca, Mg, cu, zn, Fe etc., Soil structure can be improved, increase soil fertility, promoting the growth of crop, is a kind of well organic fertilizer resource.
Summary of the invention
For the deficiencies in the prior art, the invention provides a kind of the Se-enriched bio preparation and the preparation method that improve Soil Microorganism activity, containing elemental selenium in microbiological medicine containing rich selenium of the present invention, and elemental selenium is nano level, nontoxicity, human body is safe from harm, has antagonistic action to harmful heavy metal, have Degradation for the nitrate in vegetables, can be mixed with fertilizer, good stability, add water spray on plants blade face, reaches the rich selenium object of cereal, vegetables, fruit.And it is active that this Se-enriched bio preparation improves Soil Microorganism, improvement soil, increases the quantity of soil microorganisms, improves disease-resistant, the anti-adversity ability of fruit-vegetable plant, improves the advantages such as utilization rate of fertilizer.
Technical scheme of the present invention is as follows:
Improve a Se-enriched bio preparation for Soil Microorganism activity, feed composition is as follows, is weight part:
Sodium Selenite 8 ~ 15 parts, composite trace element 5 ~ 15 parts, potassium primary phosphate 8 ~ 20 parts, 10 ~ 25 parts, urea, peptone 8 ~ 20 parts, yeast powder 4 ~ 10 parts, NaCl8 ~ 20 part, NaOH8 ~ 15 part, NaNO 34 ~ 10 parts, NaCl10 ~ 25 part, NH 4cl4 ~ 10 part, K 2hPO 48 ~ 20 parts, extractum carnis 4 ~ 10 parts, glucose 10 ~ 25 parts, starch 10 ~ 25 parts, 8 ~ 20 parts, calcium carbonate, amino acid/11 0 ~ 25 part, 2 ~ 6 parts, saltpetre, 2 ~ 6 parts, ferrous sulfate, 10 ~ 25 parts, magnesium sulfate, trisodium citrate 25 ~ 40 parts, biochemical enzyme 10 ~ 25 parts, residual active sludge 800 ~ 896 parts.
The present invention is preferred, described active sludge take from mud that sewage treatment plants discharges after filtration, screening, after solid-liquid separation, semi-solid part is active sludge, and water ratio is 82%-84% by percentage to the quality.The residual active sludge that sewage disposal of living produces is be mixed solution containing multiple bacterium liquid.
The present invention is preferred, described composite trace element, be by etc. the following substances of quality mix: manganous sulfate, ferrous sulfate, boric acid, copper sulfate, zinc sulfate, ammonium molybdate.
The present invention is preferred, and described biochemical enzyme is phosphoglucokinase PEK, hexokinase, pyruvate kinase, pyruvate carboxylase, glutamate dehydrogenase, carbamyl phosphate synthase I or carbamyl phosphate synthase II.
Phosphoglucokinase PEK, hexokinase, pyruvate kinase, pyruvate carboxylase, glutamate dehydrogenase, carbamyl phosphate synthase I, carbamyl phosphate synthase II are prior art, all can buy in market.
The present invention is preferred, a kind of Se-enriched bio preparation improving Soil Microorganism activity, and feed composition is as follows, is weight part:
Sodium Selenite 10 ~ 12 parts, composite trace element 8 ~ 12 parts, potassium primary phosphate 10 ~ 18 parts, 15 ~ 20 parts, urea, peptone 12 ~ 18 parts, yeast powder 6 ~ 8 parts, NaCl10 ~ 15 part, NaOH10 ~ 12 part, NaNO 36 ~ 8 parts, NaCl15 ~ 20 part, NH 4cl4 ~ 10 part, K 2hPO 412 ~ 18 parts, extractum carnis 6 ~ 8 parts, glucose 15 ~ 20 parts, starch 15 ~ 20 parts, 12 ~ 18 parts, calcium carbonate, amino acid/11 6 ~ 20 parts, 3 ~ 5 parts, saltpetre, 3 ~ 5 parts, ferrous sulfate, 13 ~ 22 parts, magnesium sulfate, trisodium citrate 30 ~ 35 parts, biochemical enzyme 18 ~ 25 parts, residual active sludge 850 ~ 880 parts.
The present invention is preferred, a kind of Se-enriched bio preparation improving Soil Microorganism activity, and feed composition is as follows, is weight part:
Sodium Selenite 11 parts, composite trace element 10 parts, potassium primary phosphate 14 parts, 16 parts, urea, peptone 15 parts, yeast powder 7 parts, NaCl12 part, NaOH10 part, NaNO 37 parts, NaCl16 part, NH 4cl8 part, K 2hPO 415 parts, extractum carnis 7 parts, glucose 16 parts, starch 17 parts, 15 parts, calcium carbonate, amino acid/11 8 parts, 4 parts, saltpetre, 4 parts, ferrous sulfate, 18 parts, magnesium sulfate, trisodium citrate 32 parts, biochemical enzyme 20 parts, residual active sludge 860 parts.
The preparation method of the Se-enriched bio preparation of above-mentioned raising Soil Microorganism activity, comprises step as follows:
Raw material Sodium Selenite, composite trace element, potassium primary phosphate, urea, peptone, yeast powder, NaCl, NaOH, NaNO is got by proportioning 3, NaCl, NH 4cl, K 2hPO 4, extractum carnis, glucose, starch, calcium carbonate, amino acid, saltpetre, ferrous sulfate, magnesium sulfate, trisodium citrate and biochemical enzyme, raw material is added in deionized water, be uniformly mixed, obtain mixture, mixture is added in residual active sludge and stirs, in temperature 30 ~ 35 DEG C, oxygen free condition bottom fermentation 15-25d, when mixture color changes to redness, obtain Se-enriched bio preparation, described Se-enriched bio preparation is containing elemental selenium 0.3 ~ 0.4g/L, described selenium is nano level, and median size is 60 ~ 150nm.
The present invention is preferred, and pass into rare gas element in anaerobic fermentation process and maintain oxygen-free environment, described rare gas element is nitrogen or argon gas.
The present invention is preferred, and the add-on of deionized water is raw material Sodium Selenite, composite trace element, potassium primary phosphate, urea, peptone, yeast powder, NaCl, NaOH, NaNO 3, NaCl, NH 4cl, K 2hPO 4, extractum carnis, glucose, starch, calcium carbonate, amino acid, saltpetre, ferrous sulfate, magnesium sulfate, trisodium citrate and biochemical enzyme total mass 10 ~ 30%.
The Se-enriched bio preparation that the present invention obtains is in the concentrated thing state between liquid and circle, and solution takes on a red color and shows the existence of selenium nano particles.
The application of above-mentioned Se-enriched bio preparation, for the proportion of crop planting fertilising use in scarce selenium, poor selenium area, root fertilising or leaf dressing during fertilising, during root fertilising, Se-enriched bio preparation is directly used, used by Se-enriched bio preparation diluent 2 ~ 5 times during leaf dressing, described farm crop are grape, apple, summer squash, capsicum, cucumber, Chinese cabbage rape, tomato, eggplant or rape.
Preferred further, described root fertilising, Se-enriched bio preparation Direct Uniform ground cloth is sprinkled upon in crop root part surrounding soil, earthing covers tightly again, or decile some ditches or hole, cave centered by crop root part, even for Se-enriched bio preparation cloth is sprinkled upon in bar ditch or hole, cave, then earthing covers tightly, rate of fertilizer application: Se-enriched bio preparation 80 ~ 110g/ strain.
The present invention is preferred, and described starch is W-Gum, wheat starch or sweet potato starch.
The residual active sludge that the present invention uses, takes from city domestic sewage treatment plant.
Potassium primary phosphate of the present invention, urea, peptone, yeast powder, NaCl, NaOH, NaNO 3, NaCl, NH 4cl, K 2hPO 4, extractum carnis, glucose, starch, calcium carbonate, amino acid, saltpetre, ferrous sulfate, magnesium sulfate, trisodium citrate, be conventional commercial products.
Sodium Selenite used in the present invention is purchased from Jinan Zhuan Meng Bioisystech Co., Ltd.
Beneficial effect
The standby microbiological medicine containing rich selenium advantage of the residual active sludge fermentation that the present invention adopts sanitary sewage disposal to produce is as follows:
1, the preparation method of microbiological medicine containing rich selenium of the present invention, is that the residual active sludge that produced by sanitary sewage disposal and mixing raw material fermentation are obtained, substantially increases the service efficiency of microbiological medicine containing rich selenium, saved cost.
2, microbiological medicine containing rich selenium of the present invention, not only can improve the fermenting speed of microbiological medicine containing rich selenium, and makes the particle diameter of selenium nano particle more even, is more of value to the absorption of plant to this microbiological medicine containing rich selenium.
3, microbiological medicine containing rich selenium of the present invention can increase the quantity of Soil Microorganism and improve the activity of Soil Microorganism.This type of bacterial classification causes farm crop to obtain specific fertilizer effect with its vital movement and meta-bolites thereof, growth regulating effect, the agricultural prods of the effects such as diseases prevention worm effect and soil organisms reparation.
4, rich selenium preparation of the present invention, except containing selenium element, also containing the nitrogen that plant is required, phosphorus, potassium and trace element, have production-increasing function to crop.
5, rich selenium preparation of the present invention has degeneration-resistant effect for diseases and pests of agronomic crop, and the residual active sludge that the sanitary sewage disposal contained by it produces has antagonistic action to harmful heavy metal, has Degradation, can reduce applications of pesticide amount for the nitrate in vegetables.
6, use rich selenium preparation of the present invention to be fertilizer, containing elemental selenium in rich selenium preparation, and elemental selenium is nano level, and the rich selenium product of planting out contains nano level elemental selenium, selenium content is at 30 ~ 50 micro-gs/kg, and mouthfeel is good, rich in sugar, antioxidant property is good, and nontoxicity is safe from harm to human body.
7, rich selenium preparation of the present invention is also containing living microorganism, and farm crop can be caused to obtain the agricultural prods of the effects such as specific fertilizer effect, growth regulating effect, diseases prevention worm effect and soil organisms reparation with its vital movement and meta-bolites thereof, improving Soil structure and soil microenvironment, culture fertility, raising chemical fertilizer utilization ratio and reduction fertilizer and pesticide amount of application, preventing and treating in soil continuous cropping obstacle and raising crop yield quality etc. and can play the irreplaceable effect of other fertilizer.The root of plant can fix plant, moisture and the mineral salt be dissolved in the water and nitrogen can be absorbed again from soil, used for plant-growth, root system also can the material such as synthesizing amino acid, vegeto-alkali, organonitrogen, plant hormone, and healthy and strong flourishing root is that plant is necessary.
8, the mud that sanitary sewage disposal produces is typical organic sludge, and its characteristic is organic content high (60%-80%), and particle thin (0.02-0.2mm), density little (1.002-1,0069/cm3), in colloidal structure.Containing more organism in mud, the organic content in China's mud is about 50%-70%, but carbohydrate content is higher, belongs to Hi CHO lower fat type.Again containing nutritive element and plant necessary various micro-ca, Mg, cu, zn, Fe etc. such as abundant N, P, K in mud, can improve Soil structure, increase soil fertility, promote the growth of crop, be a kind of well organic fertilizer resource.For the mud normally residual active sludge of Land_use change, the Land_use change of mud is actively a kind of.The effectively sludge treatment mode of safety.
9, mud is after anaerobic digestion, reaches the object of sludge reduction, also reclaims a part of energy simultaneously, alleviates subsequent disposal burden.The grown on soil applying sludge composting leads to dish, and the biomass of crop significantly increases, and especially on the soil of fertilizer deficiency, effect of increasing production is more obvious.Be applied to radish and cabbage heart, output significantly improves, and part composting production effect of increasing production is better than chemical fertilizer, and effect of increasing production is more obvious.
Embodiment
Below in conjunction with embodiment, technical scheme of the present invention is described further, but institute of the present invention protection domain is not limited thereto.
Embodiment 1
Improve a Se-enriched bio preparation for Soil Microorganism activity, feed composition is as follows, is weight part:
Sodium Selenite 11 parts, composite trace element 10 parts, potassium primary phosphate 14 parts, 16 parts, urea, peptone 15 parts, yeast powder 7 parts, NaCl12 part, NaOH10 part, NaNO 37 parts, NaCl16 part, NH 4cl8 part, K 2hPO 415 parts, extractum carnis 7 parts, glucose 16 parts, starch 17 parts, 15 parts, calcium carbonate, amino acid/11 8 parts, 4 parts, saltpetre, 4 parts, ferrous sulfate, 18 parts, magnesium sulfate, trisodium citrate 32 parts, biochemical enzyme 20 parts, residual active sludge 860 parts.
Preparation method:
Raw material Sodium Selenite, composite trace element, potassium primary phosphate, urea, peptone, yeast powder, NaCl, NaOH, NaNO is got by proportioning 3, NaCl, NH 4cl, K 2hPO 4, extractum carnis, glucose, starch, calcium carbonate, amino acid, saltpetre, ferrous sulfate, magnesium sulfate, trisodium citrate and biochemical enzyme, raw material is added in the deionized water of material 20%, be uniformly mixed, obtain mixture, mixture is added in residual active sludge and stirs, in temperature 32 DEG C, oxygen free condition bottom fermentation 20d, when mixture color changes to redness, obtain Se-enriched bio preparation, described Se-enriched bio preparation is containing elemental selenium 0.3 ~ 0.4g/L, described selenium is nano level, and median size is 60 ~ 150nm.
Embodiment 2
Improve a Se-enriched bio preparation for Soil Microorganism activity, feed composition is as follows, is weight part:
Sodium Selenite 8 parts, composite trace element 5 parts, potassium primary phosphate 8 parts, 10 parts, urea, peptone 8 parts, yeast powder 4 parts, NaCl8 part, NaOH8 part, NaNO 34 parts, NaCl10 part, NH 4cl4 part, K 2hPO 48 parts, extractum carnis 4 parts, glucose 10 parts, starch 10 parts, 8 parts, calcium carbonate, amino acid/11 0 part, 2 parts, saltpetre, 2 parts, ferrous sulfate, 10 parts, magnesium sulfate, trisodium citrate 25 parts, biochemical enzyme 10 parts, residual active sludge 800 parts.
Preparation method is with embodiment 1.
Embodiment 3
Improve a Se-enriched bio preparation for Soil Microorganism activity, feed composition is as follows, is weight part:
Sodium Selenite 12 parts, composite trace element 12 parts, potassium primary phosphate 18 parts, 20 parts, urea, peptone 18 parts, yeast powder 8 parts, NaCl15 part, NaOH12 part, NaNO 38 parts, NaCl20 part, NH 4cl10 part, K 2hPO 418 parts, extractum carnis 8 parts, glucose 20 parts, starch 20 parts, 18 parts, calcium carbonate, 20 parts, amino acid, 5 parts, saltpetre, 5 parts, ferrous sulfate, 22 parts, magnesium sulfate, trisodium citrate 35 parts, biochemical enzyme 25 parts, residual active sludge 880 parts.
Preparation method is with embodiment 1.
Comparative example
A kind of Se-enriched bio preparation, feed composition is as follows, is weight part:
Sodium Selenite 11 parts, composite trace element 10 parts, potassium primary phosphate 14 parts, 16 parts, urea, peptone 15 parts, yeast powder 7 parts, NaCl12 part, NaOH10 part, NaNO 37 parts, NaCl16 part, NH 4cl8 part, K 2hPO 415 parts, extractum carnis 7 parts, glucose 16 parts, starch 17 parts, 15 parts, calcium carbonate, amino acid/11 8 parts, 4 parts, saltpetre, 4 parts, ferrous sulfate, 18 parts, magnesium sulfate, trisodium citrate 32 parts, biochemical enzyme 20 parts.
Preparation method is with embodiment 1.
The effect test of each Se-enriched bio formulation application of experimental example on cole crop
To embodiment 1 ~ 3 and comparative example, field rape experiment is carried out in Xi Xin village village, Linshu County, Linyi in October, 2014, first dilute when using, extension rate is 5 times, then according to every strain 100g (undiluted) equably cloth be sprinkled upon in crop root part surrounding soil, then earthing covers tightly.Rape growing way the results are shown in Table 1:
The each biotechnological formulation of table 1 is to the application effect of plant (rape)
Project Plant growing way Output
Embodiment 1 Leaf dark green, growing way are healthy and strong 65.31
Embodiment 2 Leaf dark green, growing way are healthy and strong 67.84
Embodiment 3 Leaf dark green, growing way are healthy and strong 67.78
Control group (not using biotechnological formulation) Blade, growing way are poor 50.91
Comparative example Leaf dark green, growing way are general 53.45
As can be seen from Table 1, under same test conditions, Se-enriched bio preparation of the present invention can significantly improve the output of crop; And control group plant growing way is general, output is well below of the present invention.
As can be seen here, microbiological medicine containing rich selenium of the present invention can increase the quantity of Soil Microorganism and improve the activity of Soil Microorganism.This type of bacterial classification causes farm crop to obtain specific fertilizer effect with its vital movement and meta-bolites thereof, growth regulating effect, and the effects such as diseases prevention worm effect and soil organisms reparation, substantially increase plant biomass.

Claims (10)

1. improve a Se-enriched bio preparation for Soil Microorganism activity, feed composition is as follows, is weight part:
Sodium Selenite 8 ~ 15 parts, composite trace element 5 ~ 15 parts, potassium primary phosphate 8 ~ 20 parts, 10 ~ 25 parts, urea, peptone 8 ~ 20 parts, yeast powder 4 ~ 10 parts, NaCl8 ~ 20 part, NaOH8 ~ 15 part, NaNO 34 ~ 10 parts, NaCl10 ~ 25 part, NH 4cl4 ~ 10 part, K 2hPO 48 ~ 20 parts, extractum carnis 4 ~ 10 parts, glucose 10 ~ 25 parts, starch 10 ~ 25 parts, 8 ~ 20 parts, calcium carbonate, amino acid/11 0 ~ 25 part, 2 ~ 6 parts, saltpetre, 2 ~ 6 parts, ferrous sulfate, 10 ~ 25 parts, magnesium sulfate, trisodium citrate 25 ~ 40 parts, biochemical enzyme 10 ~ 25 parts, residual active sludge 800 ~ 896 parts.
2. the Se-enriched bio preparation of raising Soil Microorganism activity according to claim 1, it is characterized in that, described active sludge take from sewage treatment plants discharge mud after filtration, screening, after solid-liquid separation, semi-solid part is active sludge, and water ratio is 82%-84% by percentage to the quality.
3. the Se-enriched bio preparation of raising Soil Microorganism activity according to claim 1, is characterized in that, described composite trace element, be by etc. the following substances of quality mix: manganous sulfate, ferrous sulfate, boric acid, copper sulfate, zinc sulfate, ammonium molybdate.
4. the Se-enriched bio preparation of raising Soil Microorganism activity according to claim 1, it is characterized in that, described biochemical enzyme is phosphoglucokinase PEK, hexokinase, pyruvate kinase, pyruvate carboxylase, glutamate dehydrogenase, carbamyl phosphate synthase I or carbamyl phosphate synthase II.
5. the Se-enriched bio preparation of raising Soil Microorganism activity according to claim 1, it is characterized in that, feed composition is as follows, is weight part:
Sodium Selenite 10 ~ 12 parts, composite trace element 8 ~ 12 parts, potassium primary phosphate 10 ~ 18 parts, 15 ~ 20 parts, urea, peptone 12 ~ 18 parts, yeast powder 6 ~ 8 parts, NaCl10 ~ 15 part, NaOH10 ~ 12 part, NaNO 36 ~ 8 parts, NaCl15 ~ 20 part, NH 4cl4 ~ 10 part, K 2hPO 412 ~ 18 parts, extractum carnis 6 ~ 8 parts, glucose 15 ~ 20 parts, starch 15 ~ 20 parts, 12 ~ 18 parts, calcium carbonate, amino acid/11 6 ~ 20 parts, 3 ~ 5 parts, saltpetre, 3 ~ 5 parts, ferrous sulfate, 13 ~ 22 parts, magnesium sulfate, trisodium citrate 30 ~ 35 parts, biochemical enzyme 18 ~ 25 parts, residual active sludge 850 ~ 880 parts.
6. the Se-enriched bio preparation of raising Soil Microorganism activity according to claim 1, it is characterized in that, feed composition is as follows, is weight part:
Sodium Selenite 11 parts, composite trace element 10 parts, potassium primary phosphate 14 parts, 16 parts, urea, peptone 15 parts, yeast powder 7 parts, NaCl12 part, NaOH10 part, NaNO 37 parts, NaCl16 part, NH 4cl8 part, K 2hPO 415 parts, extractum carnis 7 parts, glucose 16 parts, starch 17 parts, 15 parts, calcium carbonate, amino acid/11 8 parts, 4 parts, saltpetre, 4 parts, ferrous sulfate, 18 parts, magnesium sulfate, trisodium citrate 32 parts, biochemical enzyme 20 parts, residual active sludge 860 parts.
7. the preparation method of the Se-enriched bio preparation of raising Soil Microorganism activity according to claim 1, comprises step as follows:
Raw material Sodium Selenite, composite trace element, potassium primary phosphate, urea, peptone, yeast powder, NaCl, NaOH, NaNO is got by proportioning 3, NaCl, NH 4cl, K 2hPO 4, extractum carnis, glucose, starch, calcium carbonate, amino acid, saltpetre, ferrous sulfate, magnesium sulfate, trisodium citrate and biochemical enzyme, raw material is added in deionized water, be uniformly mixed, obtain mixture, mixture is added in residual active sludge and stirs, in temperature 30 ~ 35 DEG C, oxygen free condition bottom fermentation 15-25d, when mixture color changes to redness, obtain Se-enriched bio preparation, described Se-enriched bio preparation is containing elemental selenium 0.3 ~ 0.4g/L, described selenium is nano level, and median size is 60 ~ 150nm.
8. the preparation method of the Se-enriched bio preparation of raising Soil Microorganism activity according to claim 7, is characterized in that, pass into rare gas element in anaerobic fermentation process and maintain oxygen-free environment, described rare gas element is nitrogen or argon gas.
9. the preparation method of the Se-enriched bio preparation of raising Soil Microorganism activity according to claim 7, it is characterized in that, the add-on of deionized water is raw material Sodium Selenite, composite trace element, potassium primary phosphate, urea, peptone, yeast powder, NaCl, NaOH, NaNO 3, NaCl, NH 4cl, K 2hPO 4, extractum carnis, glucose, starch, calcium carbonate, amino acid, saltpetre, ferrous sulfate, magnesium sulfate, trisodium citrate and biochemical enzyme total mass 10 ~ 30%.
10. the application of the Se-enriched bio preparation of raising Soil Microorganism activity according to claim 1, for scarce selenium, the proportion of crop planting fertilising in poor selenium area is used, root fertilising or leaf dressing during fertilising, during root fertilising, Se-enriched bio preparation is directly used, during leaf dressing, Se-enriched bio preparation diluent 2 ~ 5 times is used, described farm crop are grape, apple, summer squash, capsicum, cucumber, Chinese cabbage rape, tomato, eggplant or rape, described root fertilising, Se-enriched bio preparation Direct Uniform ground cloth is sprinkled upon in crop root part surrounding soil, earthing covers tightly again, or decile some ditches or hole, cave centered by crop root part, even for Se-enriched bio preparation cloth is sprinkled upon in bar ditch or hole, cave, earthing covers tightly again, rate of fertilizer application: Se-enriched bio preparation 80 ~ 110g/ strain.
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CN110818480A (en) * 2019-11-11 2020-02-21 成都鼎泰新源农业科技有限公司 Plant organic converzyme degradation agent and preparation and use methods thereof
CN111960889A (en) * 2020-08-25 2020-11-20 北京汇园生态科技有限公司 Extracting solution for preventing and treating plant diseases and insect pests
CN113439623A (en) * 2021-06-10 2021-09-28 江南大学 Method for improving leaf vegetable yield and selenium content based on regulation and control of root system microorganisms

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