CN106729658A - A kind of amoxicillin solid dispersion and preparation method thereof - Google Patents
A kind of amoxicillin solid dispersion and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a kind of amoxicillin solid dispersion and preparation method thereof；The weight percent content of each component of the solid dispersion is as follows：The sulbactam of Amoxicillin 5~20%, 1~5%, lysozyme 0.5~1%, aesculetin 0.5~1%, Utech S 100 1.0~2.0%, sodium hydrogensulfite 0.2~0.5%, PVP 2~5%, balance of DEXTROSE ANHYDROUS.Solid dispersion prepared by the present invention has preparation process is simple, both the drug effect of Amoxicillin had been remained, the drawbacks of avoiding general enteric coated preparations local delivery of drug excessive concentration, lysozyme is added to be even more the antibacterial activity that can improve product, significantly improve bioavilability, the product good palatability for obtaining simultaneously, does not have GI irritation, can greatly promote cure rate.
Description
Technical field
The invention belongs to veterinary drug technical field, and in particular to a kind of amoxicillin solid dispersion, also relate to
A kind of preparation method of amoxicillin solid dispersion.
Background technology
Amoxicillin (Amoxicillin), also known as amoxycillin, is that semi-synthetic acidproof penbritin class resists
Raw element.The medicine has a broad antifungal spectrum, sterilizing power is strong, and effect is rapid, to main gram-positive bacteria and some negative bacteriums such as streptococcus,
Pasteurella, the staphylococcus that penicillase is not produced, Escherichia coli, salmonella, proteus mirabilis etc. are sensitive, can be extensive
Sensitive bacteria infection for treating urethra, respiratory tract and the skin of various animals.At present, it has been used in the quick of pig, ox and poultry
Sense bacterium infection.
Amoxicillin is very poor in water stability, and degrade 1-5% per hour；Amoxicillin slightly soluble in water, in 100ml
Its solubility is only 0.1 gram in water, and its dissolution rate is less than 80%；Simultaneously traditional Amoxicillin bitter, also with intestines and stomach
Excitant, solves the problems, such as stability, solubility and dissolution rate, and good palatability becomes what Wymox research faced
Problem.Current Amoxicillin formulation has tablet, powder, parenteral solution, supensoid agent etc., but big multi-form needs multiple dosing to tie up
Sufficiently long active drug concentration is held, and repetitively administered can make the increase of administration cost, and cause animal stress reaction.And be suspended
Although agent can reach good utilization ratio of drug, however, due to the presence of water in liquid preparation, the stability of its preparation is all the time
Shortcomings, injection there is a problem of in-convenience in use and high cost.The absorption site of Amoxicillin is in the upper of intestines and stomach
End, in order to overcome its stomach irritation and acid labile, therefore its most preferred dosage form should be a kind of enteric coated preparations, and said preparation is in stomach
In there is part to discharge, usual 10%~50%, so can both avoid stimulation of the medicine to stomach, optimal suppression can be reached again
Bacteria concentration plays the purpose of rapid-onset.With the wriggling of stomach, remaining part medicine can gradually pass through pylorus and enter in enteron aisle,
Dissolved in the presence of the suitable pH value of enteron aisle, discharge medicine, medicine is entered body-internal-circulation system, keep blood concentration
Balance.Therefore, a kind of Amoxicillin oral formulations with good palatability, good stability and with double release functions are prepared
The edible comfortableness of the medicine will be greatly improved.
Solid dispersion technology is that medicine is dispersed in solid carrier in forms such as particulate, crystallite or molecular staties
One new formulation technology.
Lysozyme (lysozyme), hydrolyzes also known as muramidase (muramidase) or N- acetyl mureins glycan
Enzyme (N-acetylmuramide lycanohydrlase), is that one kind is widely present in milk, saliva, tears, egg and fish
Native enzyme in ovum, with Efficient antibacterial, sterilization, antiviral bioactivity.It can decompose killing such as staphylococcus
The gram positive bacterias such as category, streptococcus.Can be as a kind of sterilized and nourishing additive agent, in medicine, food and biochemical field
Extensive use, wide market.Due to the more prominent high efficiency in terms of the pharmacological effects such as antiviral, bactericidal antiphlogistic of the enzyme and
Popularity, the focus as biomedicine field research.
The content of the invention
It is how western that one kind of offer in view of the shortcomings of the prior art of the invention can improve animal welfare, eutherapeutic hydrochloric acid for animals
Ring element solid dispersion.
It is a further object to provide the preparation method of above-mentioned veterinary doxycycline hydrochloride solid dispersion.
The technical scheme is that：A kind of amoxicillin solid dispersion, its component and percentage by weight are：Ah
The sulbactam of Amdinocillin 5~20%, 1~5%, lysozyme 0.5~1%, aesculetin 0.5~1%, Utech S-100 1.0~
2.0%, sodium hydrogensulfite 0.2~0.5%, PVP 2~5%, balance of DEXTROSE ANHYDROUS.
Preferably, said components and percentage by weight are：Amoxicillin 10%, sulbactam 2%, lysozyme 1.0%, the bark of ash
B prime 1.0%, Utech S-100 1.5%, sodium hydrogensulfite 0.4%, PVP 3%, balance of DEXTROSE ANHYDROUS.
A kind of preparation method of amoxicillin solid dispersion, comprises the following steps：
（ 1）By Utech S-100 dissolvings in ethanol, stirring is presented clear solution after 30~60 minutes；
（ 2）Sodium hydrogensulfite, Amoxicillin and sulbactam are added, is sheared 30 minutes into high speed shear homogenizer；
（ 3）It is transferred in vacuum drying chamber again, temperature is maintained at 60 DEG C to drying, and takes out, and grinds 80 mesh sieves, obtains carrier
Core layer；
（ 4）Then PVP, lysozyme, aesculetin and the DEXTROSE ANHYDROUS of recipe quantity are put into respectively, use suitable after being well mixed
Amount pure water granulation；
（ 5）60 DEG C of dryings, whole grain obtains final product amoxicillin solid dispersion.
Preferably, the weight of above-mentioned ethanol is 10~100 times of Utech S-100.
Preferably, the weight of above-mentioned ethanol is 20 times of Utech S-100.
Preferably, the consumption of above-mentioned pure water accounts for 2%~5% ratio of the amoxicillin solid dispersion gross weight.
Preferably, the consumption of above-mentioned pure water accounts for 3% ratio of the amoxicillin solid dispersion gross weight.
Due to Amoxicillin bitter, palatability is bad；On the other hand, Amoxicillin is unstable under one's belt, degradable and right
Stomach lining has excitant, and the ill symptomses such as apocleisis, the Nausea and vomiting of livestock and poultry can be caused in administration process.The present invention is used
The Amoxicillin of solid dispersion technology is water-soluble to be increased, and bioavilability is improved, while masking the bitter taste and thorn of Amoxicillin
Swash property, increased palatability, the double delivery systmes of creative invention, said preparation has part to discharge under one's belt, usual 10%~
50%, stimulation of the medicine to stomach so can have both been avoided, the mesh that optimal Mlc plays rapid-onset can be reached again
's.With the wriggling of stomach, remaining part medicine can gradually pass through pylorus and enter in enteron aisle, in the effect of the suitable pH value of enteron aisle
Lower dissolving, discharges medicine, medicine is entered body-internal-circulation system, keeps the balance of blood concentration.
Aesculetin is in itself a kind of antimicrobial, has inhibitory action to shigella dysenteriae, can be used to treat acute bacillary dysentery, and have
Relieving asthma, phlegm-dispelling functions.Simultaneously as adjuvant, the mechanism for strengthening immune response is the physical form by changing antigen, and extension is anti-
Original retention time in body；Submission ability of the stimulation MNP to antigen；Stimulate lymphocyte differentiation, increase and expand
Immune response ability.
Lysozyme is the nospecific immunity factor important in organism, with antibacterial, antiviral, strengthen immunity and rush
Enter the multi-functionals such as wound tissue's reparation.From the point of view of lysozyme practical application effect, often than laboratory good antimicrobial effect very
It is many.Its main cause is：Lysozyme has good biocompatibility, not only direct hydrolysis malignant bacteria cell membrane, Er Qiehe
Other multi-element biologic agents in combination effect in animal body, presses down bactericidal, neutralizes a toxin, and builds up health, and promotes growth；Secondly,
Environment meets the best use of condition of lysozyme just in animal body.And under lab, do not possess also and preferably embody molten
The simulated experiment of effect in bacterium enzyme animal body.
The present invention selection Amoxicillin and lysozyme perfect adaptation, can reach the effect of low dosage curative effect high, be coated with Ah not
XiLin, reduces destruction of the hydrochloric acid in gastric juice to Amoxicillin, while sustained release is formed in enteron aisle absorbing, blood concentration is maintained for a long time and controls
Treatment amount；Coating Amoxicillin, effectively reduces the bitter taste of medicine, increased the palatability of product.Hydrochloric acid in gastric juice is reduced simultaneously to Ah not
The destruction in XiLin, dissolves fast, stable in properties in water, and degradation speed is slow.Just lost after about 4 hours in water general Amoxicillin
Effect, and this product activity that 24 hours still maintain 85% in water.Sustained release is formed in enteron aisle simultaneously to absorb, and makes blood concentration long
Phase maintains therapeutic dose, while having conciliation immunologic function, recovers macrophage immunity activity.
Being combined with lysozyme coating and solid dispersion technology for the invention, significantly improves Amoxicillin
Stability, improves bioavilability, reaches the effect of double releases, does not have gastrointestinal irritation, good palatability, while reducing poison
Side effect.
Compared to existing technology, the beneficial effects of the present invention are：
(1) solid dispersion technology is used with the effect of covering of the bag using carrier, delays the hydrolysis and oxidation of Amoxicillin, improve medicine
Stability；Double delivery systmes, can both reach the purpose that optimal Mlc plays rapid-onset, turn avoid general enteric
The drawbacks of preparation local delivery of drug excessive concentration；Cover adverse drug smell and excitant；Good dispersion, greatly accelerates the molten of medicine
Go out, improve the bioavilability of medicine, make a kind of quick-acting, efficient preparation；
(2) solid dispersion of bacteriolyze ferment treatment, due to the coated synergistic effect of lysozyme, the antibacterial activity of Amoxicillin is improved
4 times, palatability is in good taste, does not have the sensation of bitter, and is very beneficial to pipe intestinal protection and nutrient absorption, solve antibiotic not by
Hydrochloric acid in gastric juice destruction, protective rate 98%, to gastrointestinal mucosa without destruction, while solid disperses, duration of efficacy is long, improves medicine
Absorption and bioavilability, with reconcile immunologic function, recover macrophage immunity activity；(3) with Amoxicillin, the bark of ash
B prime and lysozyme are the compound preparation of main component, overcome that existing single preparations of ephedrine compound preparation effect is poor, consumption is big, remain
Shortcoming high；Amoxicillin and two kinds of Drug combinations of aesculetin, can greatly improve drug susceptibility, with single drug
Compare, dosage and times for spraying is greatly decreased, while improving curative effect of medication, reduce the dosage of antibiotic, reduce
Medicine remains risk to the toxic and side effect and medicine of animal using the potential drug that process may occur, and in field of veterinary has good
Good application prospect；
(4) addition sulbactam can effectively suppress the activity of beta-lactamase, solve the resistance problems of Amoxicillin；Match somebody with somebody
Close aesculetin synergistic effect, and have relieving asthma, phlegm-dispelling functions；
(5) relative to injection, high cost, in-convenience in use, solid dispersion can be used with spice or drinking-water, using simple,
Beneficial to large-scale cultivation.
Specific embodiment
Specific embodiment of the invention is illustrated with reference to specific embodiment, but following examples are used only in detail
The present invention is illustrated, the scope of the present invention is limited never in any form.
Embodiment 1
A kind of amoxicillin solid dispersion, component and correspondence percentage by weight are：Amoxicillin 5%, sulbactam 1%,
Lysozyme 0.5%, aesculetin 0.5%, Utech S-100 1.0%, sodium hydrogensulfite 0.2%, PVP 2%, balance of anhydrous Portugal
Grape sugar.
The preparation method of the amoxicillin solid dispersion, concretely comprises the following steps：Full dose Utech S-100 is dissolved in
In 10 times of ethanol of weight, stirring is presented clear solution after 30 minutes, adds sodium hydrogensulfite, Amoxicillin and sulbactam,
After being sheared 30 minutes into high speed shear homogenizer, it is transferred in vacuum drying chamber, temperature is maintained at 60 DEG C to drying, and takes out, and grinds
The broken mistake 80 that is milled mesh sieve, obtains carrier core layer.Then the input PVP of recipe quantity, lysozyme, aesculetin and anhydrous respectively
Glucose, is pelletized after being well mixed with appropriate purified water, 60 DEG C of dryings, and whole grain obtains final product graininess amoxicillin solid point
Powder.
The consumption of above-mentioned pure water accounts for 2% ratio of graininess amoxicillin solid dispersion gross weight.
Embodiment 2
A kind of amoxicillin solid dispersion, component and correspondence percentage by weight are：Amoxicillin 10%, sulbactam 2%,
Lysozyme 0.8%, aesculetin 0.8%, Utech S-100 1.5%, sodium hydrogensulfite 0.4%, PVP 3%, balance of anhydrous Portugal
Grape sugar.
The preparation method of the amoxicillin solid dispersion, concretely comprises the following steps：Full dose Utech S-100 is dissolved in
In 20 times of ethanol of weight, stirring is presented clear solution after 40 minutes, adds sodium hydrogensulfite, Amoxicillin and sulbactam,
After being sheared 30 minutes into high speed shear homogenizer, it is transferred in vacuum drying chamber, temperature is maintained at 60 DEG C to drying, and takes out, and grinds
The broken mistake 80 that is milled mesh sieve, obtains carrier core layer.Then the input PVP of recipe quantity, lysozyme, aesculetin and anhydrous respectively
Glucose, is pelletized after being well mixed with appropriate purified water, 60 DEG C of dryings, and whole grain obtains final product graininess amoxicillin solid point
Powder.
The consumption of above-mentioned pure water accounts for 3% ratio of graininess amoxicillin solid dispersion gross weight.
Embodiment 3
A kind of amoxicillin solid dispersion, component and correspondence percentage by weight are：Amoxicillin 10%, sulbactam 2%,
Lysozyme 1.0%, aesculetin 1.0%, Utech S-100 1.5%, sodium hydrogensulfite 0.4%, PVP 3%, balance of anhydrous Portugal
Grape sugar.
The preparation method of the amoxicillin solid dispersion, concretely comprises the following steps：By total amount Utech S-100 dissolvings
In 50 times of ethanol of weight, stirring is presented clear solution after 40 minutes, adds sodium hydrogensulfite, Amoxicillin and Sulbactam
Sodium, after being sheared 30 minutes into high speed shear homogenizer, is transferred in vacuum drying chamber, and temperature is maintained at 60 DEG C to drying, and takes out,
80 mesh sieves were ground, carrier core layer was obtained.Then PVP, lysozyme, aesculetin and the nothing of recipe quantity are put into respectively
Water glucose, is pelletized after being well mixed with appropriate purified water, and 60 DEG C of dryings, whole grain obtains final product graininess amoxicillin solid
Dispersant.
The consumption of above-mentioned pure water accounts for 3% ratio of graininess amoxicillin solid dispersion gross weight.
Embodiment 4
A kind of amoxicillin solid dispersion, component and correspondence percentage by weight are：Amoxicillin 20%, sulbactam 5%,
Lysozyme 1.0%, aesculetin 1.0%, Utech S-100 2.0%, sodium hydrogensulfite 0.5%, PVP 5%, balance of anhydrous Portugal
Grape sugar.
The preparation method of the amoxicillin solid dispersion, concretely comprises the following steps：By total amount Utech S-100 dissolvings
In 100 times of ethanol of weight, stirring is presented clear solution after 60 minutes, adds sodium hydrogensulfite, Amoxicillin and Sulbactam
Sodium, after being sheared 30 minutes into high speed shear homogenizer, is transferred in vacuum drying chamber, and temperature is maintained at 60 DEG C to drying, and takes out,
80 mesh sieves were ground, carrier core layer was obtained.Then PVP, lysozyme, aesculetin and the nothing of recipe quantity are put into respectively
Water glucose, is pelletized after being well mixed with appropriate purified water, and 60 DEG C of dryings, whole grain obtains final product graininess amoxicillin solid
Dispersant.
The consumption of above-mentioned pure water accounts for 5% ratio of gross weight.
The present invention is further described or illustrated by the following examples, but is not intended to limit the invention.
Dissolution Rate Testing, is tested using the sample of embodiment 2.
Dissolving-out method：Basket method（Annex page 114 first method of Chinese veterinary pharmacopoeia 2010 edition）；
Leaching condition：Dissolution medium is 0.1mol/L hydrochloric acid solution 900ml, and rotating speed is 50 revs/min；
Sample time：Sampled respectively at the 5th, 10,20,30,40,60,90 and 120 minutes；
Detection method：UV-VIS spectrophotometry（Appendix 26 page of Chinese veterinary pharmacopoeia 2010 edition）, at 345nm wavelength
Mensuration absorbance；
Detecting instrument：Spectrophotometry instrument, model：752N types
Result see the table below 1：The dissolution test result of table 1
Even from Dissolution experiments as can be seen that being stirred 120 minutes in 0.1mol/L hydrochloric acid solutions, dissolution rate only has
34.1%, meet the requirement of release 10%~50% in stomach.
Effect judges
Test therapeutic effect observation 1. of 1 embodiment 3 to swine influenza and select Experimental agents：Described in the embodiment of the present invention 3
Amoxicillin solid dispersion.2. animal subject：There is following symptom in certain age in days piglet of pig farm 20, sick pig：Flu hair
Heat, spray nose are sneezed, submandibular lymph nodes enlargement, schneiderian membrane flush swelling, and nose stream serosity, mucus or purulence nose liquid are exhaled
Difficult, cough and asthma is inhaled, is popular pig according to comprehensive diagnos such as epidemiology, clinical symptoms, microbiology and Serological testing
Sexuality emits.Random selection 200,4 groups of average mark.
（1）Blank group：Do not carry out any treatment；
（2）Experimental group：Using solid dispersion described in the mixed feeding embodiment of the present invention 3,200g/ tons of feed is used in conjunction 5 days.
（3）Amoxicillin control group：Using commercially available 10% Amoxicillin pulvis, 200g/ tons of feed is used in conjunction 5 days
（4）Lysozyme control group：Lysozyme solid dispersion is made by oneself using mixed feeding（Lysozyme 1.0%, aesculetin 1.0%, especially
Strange S-100 2.0%, sodium hydrogensulfite 0.5%, PVP 5%, balance of DEXTROSE ANHYDROUS）, 500g/ tons of feed be used in conjunction 5 days.
The clinical sign of observation and recording laboratory animal, judges therapeutic effect in five days.3. index of assessment of curative effect cure rate：Clinical condition
Shape disappears, and spirit, appetite etc. recover normal, and category is cured, and curing number according to each group animal calculates cure rate.
It is efficient：The animal and symptom cured after being treated through medication have obvious alleviator, are judged to effectively.It is invalid
Rate：Through medication treat after clinical symptoms do not disappear, the state of an illness do not take a turn for the better and treat during died because originally dying of illness be accordingly to be regarded as it is invalid,
Statistics invalid number, calculates inefficiency.
4. result see the table below 2；
Therapeutic effect of the embodiment of the present invention 3 of table 2 to swine influenza：
As seen from the above table, the embodiment of the present invention 3 is all remarkably higher than Ah not to the treatment cure rate and effective percentage of swine influenza
XiLin control group and lysozyme control group and blank control group, and inefficiency is substantially less than other three groups.Result shows, of the invention
Swine influenza can effectively be treated.And it is observed that after the pig treatment of implementation group, clinical symptom disappearance, appetite and spiritual shape
State etc. is significantly better than that Amoxicillin control group and lysozyme control group.
Above test result indicate that, Amoxicillin and lysozyme, the compatibility application of aesculetin are remarkably improved animal disease
The cure rate of disease, good palatability.
Claims (7)
1. a kind of amoxicillin solid dispersion, it is characterised in that its component and percentage by weight are：Amoxicillin 5~
20%, 1~5% sulbactam, lysozyme 0.5~1%, aesculetin 0.5~1%, Utech S-100 1.0~2.0%, sulfurous acid
Hydrogen sodium 0.2~0.5%, PVP 2~5%, balance of DEXTROSE ANHYDROUS.
2. amoxicillin solid dispersion is planted according to claim 1, it is characterised in that the component and weight hundred
Point ratio is：Amoxicillin 10%, sulbactam 2%, lysozyme 1.0%, aesculetin 1.0%, Utech S-100 1.5%, sulfurous acid
Hydrogen sodium 0.4%, PVP 3%, balance of DEXTROSE ANHYDROUS.
3. the preparation method of amoxicillin solid dispersion is planted according to claim 1, it is characterised in that including such as
Lower step：
（ 1）By Utech S-100 dissolvings in ethanol, stirring is presented clear solution after 30-60 minutes；
（ 2）Sodium hydrogensulfite, Amoxicillin and sulbactam are added, is sheared 30 minutes into high speed shear homogenizer；
（ 3）It is transferred in vacuum drying chamber again, temperature is maintained at 60 DEG C to drying, and takes out, and grinds 80 mesh sieves, obtains carrier
Core layer；
（ 4）Then PVP, lysozyme, aesculetin and the DEXTROSE ANHYDROUS of recipe quantity are put into respectively, use suitable after being well mixed
Amount pure water granulation；
（ 5）60 DEG C of dryings, whole grain obtains final product amoxicillin solid dispersion.
4. preparation method according to claim 3, it is characterised in that the weight of the ethanol for Utech S-100 10~
100 times.
5. the preparation method according to claim 3 or 4, it is characterised in that the weight of the ethanol is the 20 of Utech S-100
Times.
6. the preparation method according to claim 3 or 4, it is characterised in that the consumption of the pure water accounts for the compound Ah Moses
2%~5% ratio of woods solid dispersion gross weight.
7. the preparation method according to claim 3 or 4, it is characterised in that the consumption of the pure water accounts for the compound Ah Moses
3% ratio of woods solid dispersion gross weight.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101011363A (en) * | 2005-09-26 | 2007-08-08 | 刘凤鸣 | Slow release tablet of amoxicillin |
| CN101934070A (en) * | 2009-07-02 | 2011-01-05 | 天津瑞普生物技术股份有限公司 | Veterinary antibacterial drug composition containing lysozyme and oligosaccharide and application thereof |
| CN102895228A (en) * | 2012-11-01 | 2013-01-30 | 哈药集团制药总厂 | Amoxicillin and sulbactam sodium medicinal composition injection and preparation method thereof |
| CN104958253A (en) * | 2015-05-28 | 2015-10-07 | 浙江长典医药有限公司 | Child type amoxicillin sulbactam sodium and low-sodium carrier medicine composition |
| CN106074543A (en) * | 2016-07-13 | 2016-11-09 | 福建傲农生物科技集团股份有限公司 | A kind of water solublity synergistic composition containing amoxicillin and application thereof |
-
2017
- 2017-01-02 CN CN201710000369.6A patent/CN106729658A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101011363A (en) * | 2005-09-26 | 2007-08-08 | 刘凤鸣 | Slow release tablet of amoxicillin |
| CN101934070A (en) * | 2009-07-02 | 2011-01-05 | 天津瑞普生物技术股份有限公司 | Veterinary antibacterial drug composition containing lysozyme and oligosaccharide and application thereof |
| CN102895228A (en) * | 2012-11-01 | 2013-01-30 | 哈药集团制药总厂 | Amoxicillin and sulbactam sodium medicinal composition injection and preparation method thereof |
| CN104958253A (en) * | 2015-05-28 | 2015-10-07 | 浙江长典医药有限公司 | Child type amoxicillin sulbactam sodium and low-sodium carrier medicine composition |
| CN106074543A (en) * | 2016-07-13 | 2016-11-09 | 福建傲农生物科技集团股份有限公司 | A kind of water solublity synergistic composition containing amoxicillin and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| 李范珠: "《药剂学第1版》", 28 February 2011, 中国中医药出版社 * |
| 梁承远等: "秦皮乙素及其衍生物的合成与药理学研究进展", 《陕西科技大学学报》 * |
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Application publication date: 20170531 |