JPH0656689A - Preventing and treating composition for infectious diarrhea - Google Patents

Abstract

PURPOSE:To obtain a preventing and treating composition for infectious diarrhea capable of feeding a tea extract effective against a pathogen of the infectious diarrhea to the intestinal tract while keeping the activity thereof. CONSTITUTION:This preventing and treating composition for infectious diarrhea comprising chitin containing a tea extract is administered to a human or livestock to effectively prevent or treat viral or bacterial diarrhea.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a composition for preventing and treating infectious diarrhea, which comprises chitin containing a tea extract.

[0002]

BACKGROUND OF THE INVENTION Diarrhea is one of the common diseases in humans and domestic animals, but often causes severe conditions such as dehydration and other complications in young individuals, especially in the nursing period. . The causes of diarrhea are divided into those that are infectious by viruses and bacteria and those that are non-infectious due to environment, stress, diet, etc. In particular, infectious diarrhea is transmitted to other individuals and causes a large amount in the entire population. It often causes damage.

Pathogens of infectious diarrhea are roughly classified into viruses and bacteria. It develops when these pathogens enter the intestinal tract orally along with food or drink, and colonize and grow on the intestinal mucosa. In addition, it is not uncommon for two or more pathogens to undergo multiple infections and to make the symptoms more serious.

Rotavirus, coronavirus, nook virus, calicivirus, adenovirus, astrovirus and the like are known as pathogens of viral diarrhea. Diarrhea is a problem. Although active research is being conducted on vaccines against viral diarrhea, effective vaccines have not yet been successful. In the clinical setting, only symptomatic treatments such as supplementation of electrolytes and nutrients to prevent dehydration are performed.

Further, as pathogens of bacterial diarrhea, Shigella, Salmonella, pathogenic E. coli, toxigenic E. coli,
Cholera, Vibrio parahaemolyticus, Yersinia, Campylobacter, Helicobacter, Staphylococcus, Staphylococcus aureus, Pseudomonas aeruginosa and the like are known. Chemotherapy such as administration of antibiotics is usually applied to these diarrhea-causing bacteria, but there are many problems such as emergence of resistant bacteria.

On the other hand, tea has been reported as a known natural product showing an antibacterial action in vitro against many pathogenic bacteria of intestinal infectious diseases (Japanese Journal of Bacteriology, 44,669-6).
72, 1989)). In addition, it has been reported that a tea extract exhibits an infection inhibitory activity in vitro against rotavirus, which is a pathogenic virus for infant vomiting diarrhea (37th Annual Meeting of the Japanese Society for Virology, 1989). In any case, it is considered that the activity of the pathogen is reduced by binding mainly the polyphenol compound contained in the tea extract to the surface of the pathogen of diarrhea in vitro.

[0007]

Infectious diarrhea causes great damage to humans and livestock. No effective vaccine has yet been developed against the virus, the main pathogen of infectious diarrhea. In addition, antibiotics are used for pathogenic bacteria, but there are many problems such as the emergence of resistant bacteria. On the other hand, tea extracts are currently attracting attention as a natural product effective against these pathogens of infectious diarrhea by in vitro experiments. However, the polyphenol compound, which is the active ingredient in the tea extract, has the property of being very easily adsorbed to high molecular substances such as proteins and polysaccharides in foods, or cells and tissues. Therefore, if the tea extract is orally administered as it is, the active ingredient will be adsorbed to substances other than these pathogens, and it has been impossible to deliver it to the intestinal tract, which is the site of infection of the pathogen, while maintaining its activity. there were. In fact, for infants,
As a result of testing whether green tea was effective against rotavirus diarrhea, it was reported that there was no preventive effect (Japan Pediatric Society General Assembly, 1990). The present inventors also conducted animal experiments using mice, but were not able to demonstrate the efficacy against rotavirus diarrhea.

An object of the present invention is to provide a composition for preventing and treating infectious diarrhea, which can deliver a tea component effective against a pathogen of infectious diarrhea to the intestinal tract while maintaining its activity. is there.

[0009]

Means for Solving the Problems The inventors of the present invention have conducted various studies to solve the above problems, and as a result, a tea extract was contained in chitin and orally administered,
For the first time in vivo, we succeeded in preventing and treating infectious diarrhea. Furthermore, they have succeeded in producing a composition for preventing and treating infectious diarrhea containing chitin containing a tea extract.
That is, the present invention relates to a composition for preventing and treating infectious diarrhea, which comprises chitin containing a tea extract.

The tea extract of the present invention means non-fermented tea such as green tea, semi-fermented tea such as oolong tea, fermented tea such as black tea, water (including hot water), ethanol, methanol, propyl alcohol, acetone, acetic acid. It is extracted using a solvent such as ethyl or hexane, but there is no problem even if the extract is further purified to increase the purity of the polyphenol compound as an active ingredient. For example, a hot water extract of green tea, Sanphenon (registered trademark of Taiyo Kagaku Co., Ltd.) can be used.

The chitin of the present invention means poly N-acetyl-
D-glucosamine and its deacetylated product, chitosan,
Furthermore, salts formed with chitin and acids, such as acetate, hydrochloride, nitrate, phosphate, etc., -OH group or -CH2 OH group of glucosamine residue is esterified, etherified, carboxymethylated, A hydroxymethylated or O-ethylated chitin derivative is also included.

Deacetylation in the case of the above-mentioned deacetylated product of poly N-acetyl-D-glucosamine is carried out by poly N-
It can be performed by a known method of treating acetyl-D-glucosamine with an alkali. The degree of deacetylation can be adjusted by appropriately changing the alkali concentration, the treatment temperature, the treatment time and the like used at this time.

The chitin used in the present invention can be used as a powder as it is, or can be used as a molded product. The molded body is, for example, a fiber, fibril, film, porous body, microbead, or the like, and can be prepared by a known method such as dissolving chitin powder in a suitable solvent to form a dope and coagulating and molding.

The tea extract-containing chitin of the present invention refers to chitin in which the tea extract is adsorbed or chemically bound. Further, the form thereof can be processed into any form such as powder form, fiber form, fibril form, film form, porous form, and microbead form.

The method for incorporating the tea extract containing polyphenols into chitin is not particularly limited as long as it is a mixing method capable of adsorbing or chemically binding the tea extract to chitin. For example, the following method is exemplified. 1) A method in which the tea extract is dissolved in a solvent such as water, chitin is immersed in this solution, and then dried. 2) A method in which a tea extract is kneaded in advance when molding a chitin molded body. 3) A method in which a tea extract is combined with chitin or a chitin molded product using a crosslinking agent such as an aldehyde.

Since the concentration of the tea extract contained in chitin is appropriately selected depending on the target pathogen, it is not limited to a certain range and can be prepared at any concentration. For example, when a tea extract containing a large amount of tea extract is required, it is possible to adsorb 50% or more of the tea extract weight based on the total weight.

The infectious diarrhea as referred to in the present invention means viral diarrhea caused by rotavirus, coronavirus, nook virus, calicivirus, adenovirus, astrovirus, etc., and Shigella, Salmonella, pathogenicity. It refers to bacterial diarrhea whose pathogens are Escherichia coli, toxigenic Escherichia coli, cholera, Vibrio parahaemolyticus, Yersinia, Campylobacter, Helicobacter, Staphylococcus, Staphylococcus aureus, Pseudomonas aeruginosa and the like.

The subject to which the composition for preventing and treating infectious diarrhea of the present invention is administered or fed includes humans or livestock. In the case of humans, infants and young children under the age of 3 who are prone to infectious diarrhea, especially rotavirus diarrhea, are the primary target, but in the case of pathogens that cause diarrhea due to food poisoning or the like, adults are also in that category. There is no particular limitation in terms of age. In addition, in the case of livestock, cattle, horses, pigs, whose damage due to diarrhea is a problem,
It can target chickens, sheep, and goats, but especially cattle, horses, and pigs in the feeding period are easily infected with rotavirus, coronavirus, toxigenic Escherichia coli, etc., and the damage situation is serious.

The composition for the prevention and treatment of infectious diarrhea according to the present invention is a human artificial formula containing chitin containing tea extract,
Milk substitute, livestock feed, food or medicine (including veterinary medicine). In the case of human artificial milk preparation and livestock milk substitute, if powdered or microbead-shaped tea extract-containing chitin is mixed with artificial milk powder or milk powder substitute and suspended in water or warm water at the time of feeding Good. In the case of livestock feed, the tea extract-containing chitin may be mixed with a usual feed material and then pelletized if necessary. When it is added to food, it can be added by mixing the tea extract-containing chitin with ingredients such as confectionery, bread, jelly, pudding, beverages, liquid foods, and oral tube-fed foods. In the case of pharmaceuticals, it may be administered as tablets, granules or oral tube feeding agents containing a certain amount of chitin containing tea extract.

Regarding the added amount of the tea extract-containing chitin in the composition for preventing and treating infectious diarrhea of the present invention, the amount of the tea extract effective for preventing and treating infectious diarrhea is included. It is sufficient and not particularly limited. More specifically,
Since the component effective against the pathogen of infectious diarrhea is the polyphenol compound in the tea extract, it may be appropriately selected according to the concentration of the polyphenol compound contained in the tea extract-containing chitin. Hereinafter, the present invention will be described in more detail with reference to Examples and Test Examples, but the present invention is not limited to these Examples.

[0021]

【Example】

Example 1. Preparation of infant formula powder for preventing and treating infectious diarrhea 50 g of tea extract-containing chitin powder containing 50% of sanphenone (manufactured by Taiyo Kagaku Co., Ltd.) as a tea extract and 9,950 g of infant formula powder powder on the market were homogeneously mixed. , 10 kg of infant formula powder for the prevention and treatment of infectious diarrhea was obtained.

Example 2. Preparation of baked confectionery for preventing and treating infectious diarrhea A baked confectionery (cookie) was prepared by adding a tea extract-containing chitin powder containing 50% of sunphenone (manufactured by Taiyo Kagaku) as a tea extract. In addition, as a control, cookies with only added sunphenone were also prepared, and sensory tests were performed on these cookies for comparison. The formulation is shown in Table 1, and the result of the sensory test is shown in Table 2.

[0023]

[Table 1]

[0024]

[Table 2]

Example 3. Preparation of Water Confectionery (Jelly) for Prevention and Treatment of Infectious Diarrhea A water confectionery (jelly) was prepared by adding a tea extract-containing chitin powder containing 50% of sunphenone (manufactured by Taiyo Kagaku Co., Ltd.) as a tea extract. In addition, as a control, jelly containing only sanphenone was also prepared, and a sensory test was performed on these jellies for comparison. The formulation is shown in Table 3, and the result of the sensory test is shown in Table 4.

[0026]

[Table 3]

[0027]

[Table 4]

Example 4. Preparation of piglet milk substitute for preventing and treating infectious diarrhea A piglet milk substitute was prepared by adding a tea extract-containing chitin powder containing 50% of sunphenone (manufactured by Taiyo Kagaku) as a tea extract. The prescription is shown in Table 5.

[0029]

[Table 5]

Example 5. Preparation of Tablet for Preventing and Treating Infectious Diarrhea A tablet for preventing and treating infectious diarrhea containing chitin containing tea extract containing 50% of sanphenone (manufactured by Taiyo Kagaku Co., Ltd.) as a tea extract was prepared. . As a control, tablets to which only sanphenone was added were also prepared, and sensory tests were conducted on these tablets for comparison. The formulation is shown in Table 6 and the result of the sensory test is shown in Table 7.

[0031]

[Table 6]

[0032]

[Table 7]

Example 6. Preparation of Oral Tube-fed Food for Prevention and Treatment of Infectious Diarrhea An oral tube-fed food was prepared by adding chitin powder containing tea extract containing 50% of Sanphenone (manufactured by Taiyo Kagaku Co., Ltd.) as a tea extract. As a control, oral tube-fed nutritional foods containing only sanphenone were also prepared, and sensory tests were conducted on these foods for comparison. The formulation is shown in Table 8 and the result of the sensory test is shown in Table 9.

[0034]

[Table 8]

[0035]

[Table 9]

Test Example 1. Rotavirus Diarrhea Preventive Effect of Tea Extract-Containing Chitin A suspension of tea extract-containing chitin powder containing 40% of sunphenone (manufactured by Taiyo Kagaku Co., Ltd.) as a tea extract (40 mg /
(50 ml of PBS) was orally administered to 5 day old ddy mice. One hour later, to the same mouse, 50 μl of a culture supernatant containing 4 × 10 7 FCFU / ml of rotavirus.
After oral infection, the onset of diarrhea was observed for 5 days. Also, as a control, Sanphenone 20 mg / ml PB
After oral administration of S, the same infection experiment was performed. As a result, as shown in Table 10, in the group administered with the tea extract-containing chitin, the development of diarrhea caused by rotavirus was almost suppressed, whereas in the group administered with only sunphenone containing no chitin, the diarrhea preventive effect was obtained. There was no.

[0037]

[Table 10]

Test Example 2. Rotavirus diarrhea therapeutic effect of chitin containing tea extract 50 μl of the rotavirus culture supernatant used in Test Example 1 was orally infected to 5-day-old ddy mice, and 1 hour and 24 hours later, Test Example 50 μl of the same sample as 1 was orally administered, and the presence or absence of subsequent diarrhea was observed for 5 days. As a result, as shown in Table 11, diarrhea was cured on the 3rd day in the group administered with the tea extract-containing chitin, whereas all the groups administered with only chiphen-free sanphenone were treated even on the 5th day. Diarrhea was seen in the individual.

[0039]

[Table 11]

[0040]

The main pathogens of infectious diarrhea are viruses and bacteria. In vitro experiments have indicated the effectiveness of tea extracts against these pathogens. However, the polyphenol compound, which is the main active ingredient, has a property of being easily adsorbed to proteins and the like, and even if administered as it was, it could not be delivered to the intestinal tract, which is the site of infection, while maintaining its activity. Under such circumstances, the present invention has succeeded in the prevention and treatment of infectious diarrhea for the first time by orally administering chitin containing tea extract into a living body. Use of the composition containing the tea extract-containing chitin of the present invention can effectively prevent and treat infectious diarrhea.

 ─────────────────────────────────────────────────── ─── Continuation of the front page (72) Inventor Yutaka Ogasawara 9-5 Akahorishinmachi, Yokkaichi-shi, Mie Solar Chemistry Co., Ltd. (72) Inventor Yu Fujiki 9-5 Akahorishinmachi, Yokkaichi-shi, Mie Solar Chemistry Incorporated (72) Inventor Kim Takehisa 9-5 Akahorishinmachi, Yokkaichi-shi, Mie Solar Engineering Co., Ltd.

Claims (3)

[Claims] 1. A composition for preventing and treating infectious diarrhea, which comprises chitin containing a tea extract. 2. The composition for preventing and treating infectious diarrhea according to claim 1, wherein the infectious diarrhea is caused by a virus. 3. The composition for preventing and treating infectious diarrhea according to claim 1, wherein the infectious diarrhea is caused by bacteria.