CN106722947A - A kind of composite enzyme with loquat skin as raw material and preparation method thereof - Google Patents
A kind of composite enzyme with loquat skin as raw material and preparation method thereof Download PDFInfo
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- CN106722947A CN106722947A CN201611138509.8A CN201611138509A CN106722947A CN 106722947 A CN106722947 A CN 106722947A CN 201611138509 A CN201611138509 A CN 201611138509A CN 106722947 A CN106722947 A CN 106722947A
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- fermentation
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- loquat skin
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- 241001092070 Eriobotrya Species 0.000 title claims abstract description 37
- 235000009008 Eriobotrya japonica Nutrition 0.000 title claims abstract description 37
- 238000002360 preparation method Methods 0.000 title claims abstract description 15
- 239000002994 raw material Substances 0.000 title claims abstract description 15
- 102000004190 Enzymes Human genes 0.000 title claims abstract description 10
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 10
- 239000002131 composite material Substances 0.000 title claims abstract description 9
- 238000000855 fermentation Methods 0.000 claims abstract description 60
- 230000004151 fermentation Effects 0.000 claims abstract description 60
- 235000021552 granulated sugar Nutrition 0.000 claims abstract description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 8
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 7
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims abstract description 7
- 150000001875 compounds Chemical class 0.000 claims description 7
- 239000002054 inoculum Substances 0.000 claims description 7
- 239000012467 final product Substances 0.000 claims description 5
- 239000012530 fluid Substances 0.000 claims description 5
- 239000000463 material Substances 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 10
- 230000003026 anti-oxygenic effect Effects 0.000 abstract description 8
- 238000004904 shortening Methods 0.000 abstract description 3
- 230000000052 comparative effect Effects 0.000 description 5
- 230000003064 anti-oxidating effect Effects 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 240000002234 Allium sativum Species 0.000 description 2
- 240000002044 Rhizophora apiculata Species 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 239000003963 antioxidant agent Substances 0.000 description 2
- 230000003078 antioxidant effect Effects 0.000 description 2
- 235000006708 antioxidants Nutrition 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 239000000287 crude extract Substances 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 235000004611 garlic Nutrition 0.000 description 2
- 239000003112 inhibitor Substances 0.000 description 2
- 244000241235 Citrullus lanatus Species 0.000 description 1
- 235000012828 Citrullus lanatus var citroides Nutrition 0.000 description 1
- 240000008790 Musa x paradisiaca Species 0.000 description 1
- 235000018290 Musa x paradisiaca Nutrition 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000010985 leather Substances 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention provides a kind of preparation method of the composite enzyme with loquat skin as raw material, it is characterised in that the preparation method comprises the following steps：（1）By weight, the following raw material is prepared：10 12 parts of loquat skin, 13 parts of Cortex Bulbus Allii, 0.5 1 parts of granulated sugar；（2）Loquat skin and Cortex Bulbus Allii are crushed, 200 mesh sieves are crossed, 20 25 parts of water are added；（3）To step（2）Gains access the Saccaromyces cerevisiae for having activated and are fermented, and fermentation temperature is 16 18 DEG C, and pH is 34, and fermentation time is 12 15 days；（4）Adjustment fermentation temperature is 20 22 DEG C, and pH is 5 5.5, and fermentation time is 46 days.Present invention also offers composite enzyme prepared by the above method.The present invention has been greatly shortened the fermentation period of loquat skin, and obtains the composite enzyme with outstanding antioxygenic property；The shortening of Cortex Bulbus Allii in the present invention to fermentation period have it is further promote left and right, and can gained ferment antioxygenic property.
Description
Technical field
The invention belongs to enzyme fermentation engineering field, and in particular to a kind of composite enzyme and its preparation with loquat skin as raw material
Method.
Background technology
Loquat is one of fruit that people like, the market demand is huge, and not only pulp is delicious for loquat, and its discarded object Pi
Rake skin also has preferable antioxidant content.However, taking organic examination the current extraction for anti-oxidant cost in loquat skin more
The method that agent is extracted, such as《Loquat skin and mangrove bark crude extract antioxidation activity are studied》With《Loquat color of the leather extract it is external
Antioxidation activity is studied》.The composition extracted by organic reagent often needs to carry out purification process, it is difficult to extensive to prepare accordingly
Oxidation resistant product, it is often more important that, organic reagent residual there is great threat for health.
Fermentation process is carried out to the raw material of suitable fermentation using zymophyte, with can be with the advantage of mass disposal.However,
For in terms of being fermented as raw material using loquat skin, current report is little.In the preliminary experiment that inventor is done, profit
Loquat skin is fermented with conventional fermentation technique, fermentation period is very long, at least needs 55 days.What is more important, institute
The antioxygenic property of fermented product is only《Loquat skin and mangrove bark crude extract antioxidation activity are studied》30 % in report are left
The right side, illustrates that the fermentation technique of routine is very low to the utilization rate of loquat skin.
Therefore, how to improve fermentation technique so that the utilization rate of loquat skin is improved, and shorten fermentation period to save
Preparation cost, as this area problem demanding prompt solution.
In addition, the antioxygenic property of loquat skin and other fruit（Such as banana, watermelon）Compared to relatively low, loquat skin how is improved
The antioxygenic property of fermentate is also the research direction of this area.
The content of the invention
In view of the shortcomings of the prior art, an object of the present invention is to provide a kind of compound ferment with loquat skin as raw material
The preparation method of element, the preparation method comprises the following steps：
（1）By weight, the following raw material is prepared：10-12 parts, Cortex Bulbus Allii 1-3 parts, granulated sugar 0.5-1 parts of loquat skin；
（2）Loquat skin and Cortex Bulbus Allii are crushed, 200 mesh sieves are crossed, 20-25 parts of water is added；
（3）To step（2）Gains access the Saccaromyces cerevisiae for having activated, and inoculum concentration is 3-5 %, is carried out
Fermentation, fermentation temperature is 16-18 DEG C, and pH is 3-4, and fermentation time is 12-15 days；
（4）Adjustment fermentation temperature is 20-22 DEG C, and pH is 5-5.5, and fermentation time is 4-6 days, takes zymotic fluid, is obtained final product described compound
Ferment.
As shown in a comparative example of the invention, only loquat skin is fermented when conventional fermentation technique is taken
When, fermentation period is up to more than 55 days, and the antioxygenic property of gained tunning is very poor, to lipovitellinin unsaturated lipid
The antioxidation activity of fat peroxotungstic acid system（AOA）No more than 12 %.
After fermentation period of the present invention is meant more than a certain fermentation time, then fermentation time long will not increase fermentation
The generation of product.
And after two-stage fermentation technology of the invention is taken, inventor has found that not only fermentation period significantly shortens, Er Qiesuo
The AOA for obtaining product is very high, reaches as high as 62.7 %.Possible the reason for is that loquat skin is at the initial stage of fermentation, and process is slower,
During mid-term, the generation of the mortifier controlled by low temperature is more, and after fermentation temperature is suitably heightened, the production of mortifier is less,
Promoting the steady of fermentation process is carried out.Associated inhibitor therein is still being separated in the middle of identification at present.
AOA can be promoted to 60% or so by the addition of garlic skin from 50% or so.This also has certain in itself with Cortex Bulbus Allii
Antioxygenic property is relevant.However, it is worthy of note that, the addition of Cortex Bulbus Allii promotes the further shortening of fermentation period, can contract
Short fermentation time about 8 days or so.Contribution due to Cortex Bulbus Allii to fermentation time reduction is not main, and possible explanation is big
The addition of garlic skin further suppress the generation of above-mentioned mortifier.This point needs inventor follow-up by isolation of inhibitors mirror
After fixing, then row sets related experiment and is verified.
Preferably, step（1）In, by weight, prepare the following raw material：11 parts of loquat skin, 2 parts of Cortex Bulbus Allii, granulated sugar
0.8 part.
Preferably, step（3）In, inoculum concentration is 4 %.
Preferably, step（3）In, fermentation time is 13 days.
Preferably, step（4）In, fermentation time is 4 days.
Composite enzyme is prepared by the above method another object of the present invention is to provide.
Beneficial effects of the present invention：
The present invention has been greatly shortened the fermentation period of loquat skin, and obtains the compound ferment with outstanding antioxygenic property
Element；The shortening of Cortex Bulbus Allii in the present invention to fermentation period have it is further promote left and right, and can gained ferment inoxidizability
Energy.
Specific embodiment
The present invention is specifically described below by embodiment, it is necessary to it is pointed out here that be that following examples are use
It is further detailed in the present invention, it is impossible to be interpreted as limiting the scope of the invention, the field is skilled in technique
Personnel still fall within protection scope of the present invention according to some nonessential modifications and adaptations that foregoing invention content is made.
Embodiment 1
（1）By weight, the following raw material is prepared：10 parts of loquat skin, 1 part of Cortex Bulbus Allii, 0.5 part of granulated sugar；
（2）Loquat skin and Cortex Bulbus Allii are crushed, 200 mesh sieves are crossed, 20 parts of water are added；
（3）To step（2）Gains access the Saccaromyces cerevisiae for having activated, and inoculum concentration is 3 %, is sent out
Ferment, fermentation temperature is 16-18 DEG C, and pH is 3-4, and fermentation time is 12 days；
（4）Adjustment fermentation temperature is 20-22 DEG C, and pH is 5-5.5, and fermentation time is 5 days, takes zymotic fluid, obtains final product the compound ferment
Element.
Embodiment 2
（1）By weight, the following raw material is prepared：12 parts of loquat skin, 3 parts of Cortex Bulbus Allii, 1 part of granulated sugar；
（2）Loquat skin and Cortex Bulbus Allii are crushed, 200 mesh sieves are crossed, 25 parts of water are added；
（3）To step（2）Gains access the Saccaromyces cerevisiae for having activated, and inoculum concentration is 5 %, is sent out
Ferment, fermentation temperature is 16-18 DEG C, and pH is 3-4, and fermentation time is 12-15 days；
（4）Adjustment fermentation temperature is 20-22 DEG C, and pH is 5-5.5, and fermentation time is 6 days, takes zymotic fluid, obtains final product the compound ferment
Element.
Embodiment 3
（1）By weight, the following raw material is prepared：11 parts of loquat skin, 2 parts of Cortex Bulbus Allii, 0.8 part of granulated sugar；
（2）Loquat skin and Cortex Bulbus Allii are crushed, 200 mesh sieves are crossed, 22 parts of water are added；
（3）To step（2）Gains access the Saccaromyces cerevisiae for having activated, and inoculum concentration is 4 %, is sent out
Ferment, fermentation temperature is 16-18 DEG C, and pH is 3-4, and fermentation time is 13 days；
（4）Adjustment fermentation temperature is 20-22 DEG C, and pH is 5-5.5, and fermentation time is 4 days, takes zymotic fluid, obtains final product the compound ferment
Element.
Comparative example 1
After loquat skin is crushed, cross 200 and sieve, add suitable quantity of water and granulated sugar, Saccaromyces cerevisiae are inoculated with, in 16-
18 DEG C of fermentations.In common reporter scope, the addition and fermentation pH of water and granulated sugar are adjusted anyway, fermentation period is 55
More than it.The AOA of gained tunning is no more than 12 %.
Comparative example 2
In addition to without Cortex Bulbus Allii, remaining is consistent with embodiment 3.The time of second level fermentation was than embodiment more than 38 days.
Experimental example
The antioxygen to lipovitellinin unrighted acid Peroxygen systems is carried out to embodiment 1-3 and the gains of comparative example 2
Change active testing, as a result as shown in table 1.
Table 1
| Embodiment 1 | Embodiment 2 | Embodiment 3 | Comparative example 2 | |
| AOA（%） | 61.9 | 61.5 | 62.7 | 51.2 |
Claims (6)
1. the preparation method of a kind of composite enzyme with loquat skin as raw material, it is characterised in that the preparation method includes as follows
Step：
（1）By weight, the following raw material is prepared：10-12 parts, Cortex Bulbus Allii 1-3 parts, granulated sugar 0.5-1 parts of loquat skin；
（2）Loquat skin and Cortex Bulbus Allii are crushed, 200 mesh sieves are crossed, 20-25 parts of water is added；
（3）To step（2）Gains access the Saccaromyces cerevisiae for having activated, and inoculum concentration is 3-5 %, is carried out
Fermentation, fermentation temperature is 16-18 DEG C, and pH is 3-4, and fermentation time is 12-15 days；
（4）Adjustment fermentation temperature is 20-22 DEG C, and pH is 5-5.5, and fermentation time is 4-6 days, takes zymotic fluid, is obtained final product described compound
Ferment.
2. preparation method according to claim 1, it is characterised in that step（1）In, by weight, prepare following former
Material：11 parts of loquat skin, 2 parts of Cortex Bulbus Allii, 0.8 part of granulated sugar.
3. preparation method according to claim 1, it is characterised in that step（3）In, inoculum concentration is 4 %.
4. preparation method according to claim 1, it is characterised in that step（3）In, fermentation time is 13 days.
5. preparation method according to claim 1, it is characterised in that step（4）In, fermentation time is 4 days.
6. the composite enzyme that the preparation method according to claim any one of 1-5 is prepared.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611138509.8A CN106722947A (en) | 2016-12-12 | 2016-12-12 | A kind of composite enzyme with loquat skin as raw material and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611138509.8A CN106722947A (en) | 2016-12-12 | 2016-12-12 | A kind of composite enzyme with loquat skin as raw material and preparation method thereof |
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| CN201611138509.8A Pending CN106722947A (en) | 2016-12-12 | 2016-12-12 | A kind of composite enzyme with loquat skin as raw material and preparation method thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107212339A (en) * | 2017-06-15 | 2017-09-29 | 台州瑞祥教育科技有限公司 | The processing method of rich glutathione loquat skin tartar sauce |
| CN107279893A (en) * | 2017-06-15 | 2017-10-24 | 台州瑞祥教育科技有限公司 | The processing method of loquat skin tartar sauce |
| CN109820091A (en) * | 2019-03-31 | 2019-05-31 | 江苏波杜农牧股份有限公司 | A kind of preparation method of Cortex Bulbus Allii particle |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1453025A (en) * | 2003-05-23 | 2003-11-05 | 李森青 | Plant leaven with hot and fragrant smell of propylene sulfide and its prepn and use |
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2016
- 2016-12-12 CN CN201611138509.8A patent/CN106722947A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1453025A (en) * | 2003-05-23 | 2003-11-05 | 李森青 | Plant leaven with hot and fragrant smell of propylene sulfide and its prepn and use |
Non-Patent Citations (2)
| Title |
|---|
| 李亚利等: "大蒜皮提取物抗氧化活性初步研究", 《食品科技》 * |
| 李琳等: "枇杷皮和芒果皮粗提液抗氧化活性研究", 《电子科技大学学报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107212339A (en) * | 2017-06-15 | 2017-09-29 | 台州瑞祥教育科技有限公司 | The processing method of rich glutathione loquat skin tartar sauce |
| CN107279893A (en) * | 2017-06-15 | 2017-10-24 | 台州瑞祥教育科技有限公司 | The processing method of loquat skin tartar sauce |
| CN109820091A (en) * | 2019-03-31 | 2019-05-31 | 江苏波杜农牧股份有限公司 | A kind of preparation method of Cortex Bulbus Allii particle |
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