KR101639604B1 - Composition for curing hangover - Google Patents

Abstract

The present invention relates to a composition for improving hangover, and more particularly, to a composition for improving hangover, which comprises a post fermentation tea extract and a chitooligosaccharide using a non-pathogenic microorganism in an appropriate ratio to produce a synergistic effect on the activation of an aldehyde dehydrogenase which degrades acetaldehyde, The present invention relates to a composition for improving the hangover, reducing the concentration of acetaldehyde in blood, protecting the liver, and improving hangover for fatigue recovery.

After fermentation tea, chitooligosaccharide, aldehyde dehydrogenase, acetaldehyde, alcohol, improve hangover, liver protection, fatigue recovery

Description

[Composition for curing hangover]

The present invention relates to a composition for improving hangover, and more particularly, to a composition for improving hangover, which comprises a post fermentation tea extract and a chitooligosaccharide using a non-pathogenic microorganism in an appropriate ratio to produce a synergistic effect on the activation of an aldehyde dehydrogenase which degrades acetaldehyde, The present invention relates to a composition for improving the hangover, reducing the concentration of acetaldehyde in blood, protecting the liver, and improving hangover for fatigue recovery.

Alcohol is a moderate means of accelerating metabolism and relieving stress, but over-consumption can lead to thirst, general boredom, fatigue, memory loss, abdominal bloating, dyspepsia, vomiting, diarrhea and vitamin deficiency. It causes a hangover. In addition, alcohol can cause blood swelling and fine blood vessel rupture. Thus, excessive intake of alcohol causes a swelling of the face and body. In addition, since cellulite also removes moisture, it becomes keratinized and becomes rough with neutral and oily skin. In addition, the next day when you usually drink a lot of swelling and rash on your face or eyes.

This hangover phenomenon is known to be caused by the action of alcohol and acetaldehyde accumulated in the hepatocytes and in the body. The ingested ethanol is absorbed through the digestive tract and reaches its peak blood concentration between 20 and 120 minutes after ingestion. Absorbed ethanol is metabolized in all organs including the liver, some excreted by breathing, urine or sweat.

Ethanol entering the liver is converted to acetate by the action of alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) in the cytoplasm, which is excreted through hepatic cells through the circulation system.

Acetaldehyde, the first metabolite of ethanol, has been found to be a major source of alcoholic liver damage due to its high reactivity and toxicity compared to ethanol. Acetaldehyde inhibits the respiration of mitochondria in the intracellular energy producing organs, inhibits oxidative phosphorylation, combines with membrane proteins and collagen proteins to produce antibodies, immunologically cytotoxic to release hepatocyte secretory proteins It has been shown to inhibit. Acetaldehyde also promotes collagen synthesis in myofibroblasts, resulting in hepatic fibrosis and metamorphosis of hepatocytes. It also reacts with macromolecules in vivo to form adducts. Particularly, type II, present in mitochondria among aldehyde dehydrogenases, is important for the removal of acetaldehyde, because Asians are generally deficient or deficient in type II enzymes, so acetaldehyde oxidation is slow and thus toxic to acetaldehyde and ethanol By this, normal metabolism is disturbed, and many hangover phenomenon is felt.

Therefore, sputum products should promote alcohol metabolism to reduce blood alcohol and acetaldehyde levels. However, there is little known about substances that enhance the activity of aldehyde dehydrogenase.

Accordingly, the present inventors have made efforts to find an effective agent capable of improving hangover by promoting the activity of acetaldehyde dehydrogenase. As a result, when the post-fermentation tea extract and chitooligosaccharide using a non-pathogenic microorganism are mixed at a proper ratio, acetaldehyde dehydrogenase And thus the present invention has been completed.

It is therefore an object of the present invention to provide a composition for improving hangover comprising an efficacy enhancing activity of acetaldehyde dehydrogenase.

To achieve the above object, the present invention provides a composition for improving hangover comprising a mixture of a post-fermented tea extract and chitooligosaccharide at a weight ratio of 1: 1 to 1: 5.

The composition for improving hangover according to the present invention increases the activity of acetaldehyde dehydrogenase in the body to promote the metabolism of alcohol in the liver, thereby lowering the concentration of acetaldehyde in the blood, thereby improving hangover, liver protection and fatigue recovery.

The term " post-fermented tea "used herein means a tea obtained by inactivating an enzyme in green tea by applying heat and then fermenting and aging the microorganism under appropriate conditions such as moisture and temperature. It is distinguished from the whole fermentation tea.

The post-fermentation tea used in the present invention is preferably prepared by fermenting green tea leaves at about 15 to 30 DEG C for about 24 to 28 days, but is not limited thereto.

The microorganism used in the production of the post-fermentation tea is preferably a non-pathogenic microorganism, more preferably a microorganism capable of being edible, for example, yeast or lactic acid bacteria. The microorganism according to the embodiment of the present invention in my process as Saccharomyces (Saccharomyces sp.), Bacillus (Bacillus sp . ) Or Aspergillus sp. ). For instance, three to Levy saccharide My process jiae (Saccharomyces cerevisiae) or saccharide in my process Carlsbad beoljen sheath (Saccharomyces calsbergensis ), but the present invention is not limited thereto.

In the present invention, the post-fermentation tea can be used in the form of water or an organic solvent-extracted extract. The organic solvent used in the present invention may be selected from the group consisting of ethanol, methanol, butanol, ether, ethyl acetate, chloroform and a mixed solvent of these organic solvents and water. For example, 70% or more of ethanol may be used. At this time, the extraction temperature may be, for example, 10 to 80 ° C, and may be extracted for 6 to 24 hours, for example. If the extraction temperature and the extraction time are exceeded, the extraction efficiency may be deteriorated or the component may be changed.

The chitooligosaccharide used in the present invention may have a molecular weight in various ranges, for example, a molecular weight of 3,000 or less, for example, a molecular weight of 700 to 2,000. For example, May have a molecular weight of 700 to 1,500, and may have a molecular weight of 700 to 1,000, for example.

The chitooligosaccharide used in the present invention may be, for example, a chitooligosaccharide of 3 to 7 sugars based on the total weight of chitooligosaccharide of 30 wt% or more.

The method for producing the chitooligosaccharide as described above is not particularly limited. For example, crabs or shrimp shells are first separated and purified by chitin by crushing, desalting, protein removal and impurity removal processes, and then deacetylated to prepare chitosan. The chitooligosaccharide can be prepared from the chitosan by a chemical decomposition method using an acid such as an inorganic acid or an organic acid, or a decomposition method using an enzyme. Commercially available chitooligosaccharide products may also be used.

When the chitooligosaccharide is produced through an enzymatic decomposition method, the enzyme which can be used is not particularly limited, but for example, a cellulase may be used. Thus, the method of producing chitooligosaccharide by enzymatic degradation of chitosan is not particularly limited. For example, after adding purified water to chitosan, 2 to 3% of lactic acid is added, and the mixture is stirred at a temperature of 40 to 60 DEG C to prepare a chitosan dispersion containing lactic acid having a solid content of 5 to 10%. The pH of the completely dissolved chitosan dispersion is adjusted to 4 to 6, and the cellulase, which is chitosanase, is dissolved in purified water and then added. Then, it is hydrolyzed at 40 to 60 ° C for 14 to 20 hours, heat-treated at 80 ° C for 30 minutes to inactivate the enzyme, and then filtered and dried to produce chitooligosaccharide.

The composition for improving hangover according to the present invention may contain the after fermentation tea extract and the chito oligosaccharide in a weight ratio of 1: 1 to 1: 5. If the content of the after fermentation tea extract is lower or higher than the above ratio, the activity of the acetaldehyde dehydrogenase is lowered.

In one embodiment of the present invention, a composition comprising a mixture of a post-fermented tea extract and chitooligosaccharide at a ratio of 1: 5 proved to significantly increase the activity of acetaldehyde dehydrogenase in vitro. The concentration of fermented tea extract and chitooligosaccharide did not increase the activity of acetaldehyde dehydrogenase when used alone. The composition containing an appropriate ratio of post-fermentation tea and chitooligosaccharide according to an embodiment of the present invention promotes the activity of acetaldehyde dehydrogenase to accelerate the decomposition of acetaldehyde, thereby reducing the concentration of acetaldehyde in blood, So that hangover can be improved quickly and easily.

The composition for improving hangover according to the present invention may contain 10 to 90% by weight of a mixture of chitooligosaccharide and post-fermented tea extract based on the total weight of the composition. The mixture may be prepared into a formulation containing various ranges of content during the manufacturing process. For example, 10 to 60% by weight is suitable for preparing tablets and soft capsules, and 10 to 90% by weight for preparing hard capsules.

In addition, the composition for improving hangover according to the present invention can be easily selected by those skilled in the art in addition to the post-fermented tea extract and chitooligosaccharide and additives or additives commonly used in the art, ≪ / RTI > In one embodiment of the present invention, the composition may be formulated in the form of a hepatic protective agent or a fatigue recovery agent promoting the decomposition of aldehyde in the body, but the present invention is not limited thereto.

Hereinafter, the present invention will be described more specifically with reference to examples and test examples. It is to be understood that the scope of the present invention is not limited to these embodiments and that variations, substitutions, and insertions conventionally known in the art can be carried out, And this is included in the scope of the present invention.

[Example 1] Preparation of post-fermented tea extract

≪ Preparation of post fermentation tea using Saccharomyces >

The cells were cultured in a vibrating incubator at < RTI ID = 0.0 > 20-30 C & Saka access to my Celebi jiae (Saccharomyces Cerevisiae ) were recovered and the strain and active medium were separated from the centrifuge at the first stage . The strains were washed once with 0.8-1.0% physiological saline and then mixed with 100 ml of sterilized water at a concentration of 2.0% by weight of sugar and 2.0% by weight of fructose, sterilized at 120 ° C for 15 minutes, The strain was added to the fermentation broth prepared by cooling to 25 占 폚. The fermentation broth, which acts as moisture and energy, is for the metabolism of suitable microorganisms. In order to facilitate the fermentation of the strain damaged during the washing process, the strain was added to the fermentation broth and stabilized by culturing in an incubator for 24 hours.

0.5% by weight of soybean powder protein was mixed with the fermentation broth to prepare a fermenting broth containing strains so that the number of fermentation broths was 10 ⁵ to 10 ⁷ CFU / mL. In the sterilized reaction tank, the fermentation broth was added to the green tea main substrate prepared for each small cell unit so as to have a concentration of 66.7% by weight based on the green tea leaf weight. Also, The green tea leaf was continuously stirred after the fermenter solution was mixed so as to prevent the strain from being damaged due to rapid temperature increase and to absorb water evenly into the green tea leaves. After 15 minutes, the reaction was completed and the temperature of the green tea fermentation broth was lowered. The growth of other strains becomes difficult at the fermentation temperature, so that the effect of suppressing the growth of other germs during the fermentation period can be expected. Fermentation was carried out for 14 days and hot air drying was performed at 80 ° C for 5 hours.

Total microbial counts in the fermented tea were below 10 ⁶ CFU / g, and no pathogenic microorganisms were detected.

≪ Preparation of post fermented tea extract >

1 kg of the fermented tea was immersed in 5 L of 70% ethanol solution, refluxed at 80 ° C for 3 hours, and then extracted at room temperature for 12 hours. The extract was filtered, concentrated under reduced pressure and lyophilized to prepare powdery post-fermented tea extract. The yield was 15 ~ 20% and the prepared powder was stored at 4 ℃ until use.

[Test Example 1] Measurement of activity of aldehyde dehydrogenase

The acetaldehyde dehydrogenase (Roche Applied Science) from the lyophilized yeast was purchased and dissolved in a solution containing 100 mM Tris-HCl (pH 8.0) and 0.02% albumin at a concentration of 0.0001 unit / ml. 0.001 units of acetaldehyde dehydrogenase was added to 40 mM acetaldehyde, 2 mM NAD (Hepes 50 mM, pH 7.5), and the absorbance was measured at 340 nm for 10 minutes.

In the same manner as above, a mixture of chitooligosaccharide, post-fermented tea extract, green tea, a mixture of chitooligosaccharide and post-fermented tea extract, and a mixture of chitooligosaccharide and common green tea together with acetaldehyde dehydrogenase, After the treatment, the activity of acetaldehyde dehydrogenase was measured.

The above experiment was repeated three times and the average activity when treated with acetaldehyde dehydrogenase was 0.062 unit / ml. When the sole activity of these enzymes was defined as 1, the activity ratio in the presence of each test substance was expressed as a ratio value, which is the enzyme activity / enzyme activity in the presence of each test substance. The results are shown in Table 1 and FIG. 1 .

Test substance Activity (Unit / ml) Control ratio Acetaldehyde dehydrogenase 0.062 1.001 Chito oligosaccharide 20 μg / ml 0.059 0.954 After fermentation tea extract 20 ㎍ / ml 0.055 0.885 Green tea extract 20 ㎍ / ml 0.053 0.860 Chito oligosaccharide 10 μg / ml + post-fermented tea extract 10 μg / ml (1: 1) 0.075 1.214 Chitooligosaccharide 10 μg / ml + post-fermented tea extract 5 μg / ml (2: 1) 0.086 1.391 Chitooligosaccharide 5 ㎍ / ml + post fermented tea extract 10 ㎍ / ml (1: 2) 0.059 0.961 Chitooligosaccharide 10 g / ml + post-fermented tea extract 2 g / ml (5: 1) 0.123 1.991 Chitooligosaccharide 2 g / ml + post-fermented tea extract 10 g / ml (1: 5) 0.052 0.932 Chitooligosaccharide 10 ㎍ / ml + green tea extract 10 ㎍ / ml (1: 1) 0.049 0.790 Chito oligosaccharide 10 / / ml + green tea extract 5 / / ml (2: 1) 0.055 0.887 Chito oligosaccharide 5 ㎍ / ml + green tea extract 10 ㎍ / ml (1: 2) 0.046 0.742 Chito oligosaccharide 10 / / ml + green tea extract 2 / / ml (5: 1) 0.051 0.823 Chito oligosaccharide 2 ㎍ / ml + green tea extract 10 ㎍ / ml (1: 5) 0.042 0.677

In the results of Table 1 and FIG. 1, when the post fermented tea extract, chito oligosaccharide and green tea were used alone, the activity of acetaldehyde dehydrogenase was not increased. In addition, the activity of enzyme was not increased even when chitooligosaccharide was mixed with common green tea. However, the activity of acetaldehyde dehydrogenase was significantly increased when the mixture of post-fermented tea extract and chitooligosaccharide was mixed at a ratio of 1: 1 to 1: 5, and especially the two substances were mixed at a ratio of 1: 5 The activity of acetaldehyde dehydrogenase was increased by about 2 times as compared with the control.

Therefore, it was confirmed that the composition obtained by mixing the post fermented tea extract and the chitooligosaccharide in an appropriate ratio can increase the activity of the acetaldehyde dehydrogenase, and can most effectively remove acetaldehyde.

FIG. 1 is a graph showing the results of measurement of acetaldehyde dehydrogenase activity of yeast after treatment of each test substance in a test tube.

Claims (10)

A composition for improving hangover comprising an after-fermented tea extract and chitooligosaccharide as an active ingredient, The mixing ratio of the after fermentation tea extract and the chitooligosaccharide is 1: 2 to 1: 5 by weight, Wherein the after-fermentation tea extract is obtained by extracting green tea leaves fermented with a non-pathogenic microorganism Saccharomyces sp. With ethanol. The method according to claim 1, Wherein the mixing ratio of the after fermentation tea extract to the chitooligosaccharide is 1: 5 by weight. The method according to claim 1, Wherein the chitooligosaccharide has a molecular weight of 3,000 or less. The method according to claim 1, Wherein the chitooligosaccharide comprises at least 30% by weight of chitooligosaccharide in an amount of 3 to 7 sugars based on the total weight of the chitooligosaccharide. delete The method according to claim 1, Wherein the non-pathogenic microorganism is Saccharomyces cerevisiae . A soy safflower comprising a composition according to any one of claims 1 to 4 and 6. A fatigue recovery agent comprising a composition according to any one of claims 1 to 4 and 6. 8. The method of claim 7, Wherein the formulation has any one of the formulations selected from the group consisting of tablets, injections, capsules, liquids, pills, syrups, and gels. 9. The method of claim 8, Wherein the fatigue recovery agent has any one of the formulations selected from the group consisting of tablets, injections, capsules, liquids, pills, syrups, and gels.

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