CN106721525A

CN106721525A - A kind of digestive utilization ratio catfish feed high

Info

Publication number: CN106721525A
Application number: CN201611060214.3A
Authority: CN
Inventors: 白金山; 白云; 王希忠; 于海波
Original assignee: Ningxia Qiangnian Fishery Technology Co ltd
Current assignee: Ningxia Qiangnian Fishery Technology Co ltd
Priority date: 2016-11-21
Filing date: 2016-11-21
Publication date: 2017-05-31

Abstract

The invention discloses a kind of digestive utilization ratio catfish feed high, belong to catfish feed processing technology field, with catfish nutritional need, based on water body environment, with reference to its digestion characteristics, optimize existing catfish feed formula, science compounds the ANFs in the catfish feed that can effectively degrade, promote starch, the utilization rate of the nutriment such as protein and mineral matter, improve the protein level of catfish, body antioxidation activity and digestibility, significantly improve catfish digestibility and production performance, reduce feed coefficient, prevent water pollution, maintain the feed complex enzyme of fine water；Catfish feed caking property, water resistance, glossiness and frost resistance are remarkably improved, wormwood seed powder of the feed containing powder rate is reduced；The calcium berry extract of calcium, iron and Soluble Fiber can effectively be supplemented；The phagostimulant of feed palatability can be greatly improved, finally prepares that a kind of feed coefficient is low, digestibility is high, production performance good, reproductive performance is high, disease-resistant performance is strong, palatability is strong, low cost catfish feed.

Description

A kind of digestive utilization ratio catfish feed high

Technical field

The present invention relates to catfish feed manufacturing, and in particular to a kind of digestive utilization ratio catfish feed high.

Background technology

Aquatic feeds are different from animal and fowl fodder, to feed formula, raw material powder particle diameter, pelleting temperature, water resistance, rate containing powder There is requirement higher.Therefore, Aquatic Feed Processing technological process also has obvious difference with animal and fowl fodder production, and feed adds Work technique directly influences the quality of aquatic feeds, such as the uniformity of feed, settleability, pulverization rate, surface gloss and its resistance to It is aqueous etc..Rational feed formula and processing technology are remarkably improved the digestibility of feed, increase the water stability of feed, The pollution that feed is caused to water body is reduced, and extends ingesting the time for fish.

Catfish is animal feeding fish, is the improved seeds in fresh-water fishes, and its is nutritious, aqueous in every 100 grams of flesh of fish Point 64.1 grams, 14.4 grams of protein, and containing several mineral materials and trace element, be particularly suitable for it is weak it is deficient, malnutritive it People eats.In addition to the roe of catfish has miscellaneous taste inedibility, whole body is precious, and catfish is rare nutrient excellent product, early in history records In just it is on the books, can be compared favourably with shark's fin, wild soft-shelled turtle, be treasure in fish.Its dietary function and medical value is other Not available for fish, strong able-bodied bone and the effect of lengthening one's life are the characteristics of it are unique.Catfish is the good merchantable brand of lactagogue, its sweet property simultaneously Temperature, and play the role of nourishing yin and nourishing blood, bowl spares gas, appetizing, diuresis, is adapted to common people and eats, especially with old, young, women's postpartum and The not good people of digestive function is applicable the most, with wide market prospects and development potentiality, as the concern of raiser in recent years Focus.

At present, the improvement to catfish culture technology is concentrated mainly on feed manufacturing aspect：Have based on animal fodder： The A of Chinese patent CN 105192479 disclose a kind of catfish feed, in parts by weight, including the following raw material：Megasse 10-20 parts, Swine blood meal 20-30 parts, fish meal 10-15 parts, shrimp med 5-10 parts, peanut 3-5 parts, earthworm 3-5 parts, rheum officinale 0.5-1 parts, Gynostemma pentaphylla 0.5-1 parts, 0.1-0.3 parts of EM bacterium, brown sugar 0.1-0.3 parts, water 5-10 parts, the invention is nutritious, add Chinese herbal medicine, The resistance against diseases of catfish can be improved.Although the single use animal fodder palatability of patent disclosed above preferably, is deposited In nutritional imbalance, relatively costly, raw material sources, transport and the difficult problem of storage.

With the increasingly fierceness of market competition, for reduces cost, plant feed ratio gradually increases in feed ingredient： The B of Chinese patent CN 103598443 disclose a kind of catfish feed, and the feed is obtained by the raw material of following weight portion：Corn 55- 60, soybean slag 20-25, dregs of beans 15-18, maize straw 12-15, black bean powder 10-12, wheat-middlings 12-15,6-8 parts of water earthworm powder, sheep Hepar siccatum 5-6, shrimp shell meal 4-5, laver powder 5-6, dry ferment 4-5, are dehydrated clover powder 6-8, asparagus lettuce leaf 6-8, proper amount of salt, food calling Agent 4-5.The catfish feed that the invention is prepared, from the omnivorous characteristic of catfish, with the addition of water earthworm, sheep liver, seaweed, asparagus lettuce Deng raw material, the nutritional ingredient needed for not only meeting the development of catfish fast-growth, while increased the palatability of feed, it is to avoid The waste of feed.The A of Chinese patent CN 104082583 disclose the catfish feed that a kind of duckweed/corn must be combined, its feature It is to be made from the following raw materials in parts by weight：Black rice face 17-19, small rice and flour 18-20, sorghum flour 16-18, potato juice 11- 13rd, glucose 4-6, duckweed 17-21, corn must 12-14, dried small shrimp 13-15, quail meat 8-10, soya bean 14-16, sesame 4-8, Moschus 0.02-0.04, monosodium glutamate 0.1-0.2, grape-kernel oil 1.2-3.2, string of bells hung round the neck grass 0.1-0.3, Radix Isatidis 0.2-0.4, ginkgo leaf 0.2-0.5, auxiliary agent 3-6, suitable quantity of water；Effectively be combined various ingredients by the feed described in the invention, and nutrition is balanced, addition duckweed, Corn must can excite the appetite of fish, fast with poly- fish, and the characteristics of not dissipating and easy to use is eaten long, and easily storage has very Considerable market application foreground.The A of Chinese patent CN 104082582 disclose a kind of catfish feed of absorption easy to digest, and it is special Levy and be, be made from the following raw materials in parts by weight：Buckwheat flour 17-19, wheat bran 20-25, mung bean face 15-17, Fructus Hordei Vulgaris powder 14-18, Banaina 6-8, aspongopus powder 7-9, streaky pork 12-14, pumpkin 18-23, glucose starch 6-9, mulberry leaf powder 11-13, citric acid 2.1-4.3, debittering honey pomelo skin 9-12, Soybean Meal 18-22, rapeseed meal 21-26, maize alcohol lees 3.6-5.8, oil chaff 11-13, scattered blood Sub- 0.1-0.3, spiceleaf 0.4-0.6, Japanese cryptotaenia stem and leaf 0.2-0.3, rhizoma nardostachyos 0.2-0.5, auxiliary agent 4-7, suitable quantity of water；The feed of the invention is easy Digestion, absorption are fast, and materials are simple and easy to get, low cost, and nutrition is balanced, can meet demand of the catfish to various nutrition；Addition corn The beneficiating ingredients such as alcohol grain, Japanese cryptotaenia stem and leaf can improve the appetite of catfish, increase its feeding rate, and then improve catfish production, increase The culture benefit of raiser.The A of Chinese patent CN 105533144 disclose a kind of catfish feed, in parts by weight, bag Include the following raw material：Maize flour 20-40 parts, brown sugar 4-6 parts, corn germ cake 20-40 parts, bean powder 3-5 parts, Chinese cabbage 10-12 parts, it is white Cool 1-3 parts, rapeseed dregs 20-40 parts, Radix Astragali 3-5 parts, fishbone dust 10-12 parts, carrot 4-6 parts, clover 10-12 parts, dandelion 4- It is 6 parts, wax gourd 6-8 parts, water 8-10 parts, nutritious, Chinese herbal medicine is added, the resistance against diseases of catfish is improved, using zymotechnique, battalion Support and be more easy to absorb.The single use plant feed of patent disclosed above or its ratio are excessive, although low cost, but there is also The problems such as palatability difference or addition phagostimulant, nutritional imbalance.

Of animal or plant nature feed is carried out into the Main way that science compounding is catfish feed advance from now on：Chinese patent CN 103704485 A disclose a kind of clarias fuscus feed and preparation method thereof, are made from the following raw materials in parts by weight：Ripe pig blood 45-50, minced fillet 12-15, fish meal 9-12, wheat bran 24-28, bean dregs 12-15, vinasse 5-6, rapeseed cake 12-15, apple leaf 8- 10th, gynostemma pentaphylla 3-4, spermacoce latifolia 1-2, dandelion 2-3, twocolor sealavander herb 2-3, folium lycii 4-5, rhizoma zingiberis 1-2, root of kudzu vine 10-12, lure Food agent 3-4, feed fermentation agent 1-2.Contain phagostimulant in the invention feed formula, the appetite of fish can be improved, increased appetite, soon Speed weightening, meanwhile, the composition such as dandelion, gynostemma pentaphylla in formula, beneficial health can be clearing heat and detoxicating, and clarias fuscus fishes eat Afterwards, human body is passed into by food chain, also beneficial health.The A of Chinese patent CN 105410477 disclose a kind of catfish Feed, in parts by weight, including the following raw material：Chinese yam 20-40 parts, mashed potatoes 25-35 parts, mock-strawberry 1-3 parts, white granulated sugar 3-5 parts, wheat flour 30-50 parts, Swine blood meal 20-40 parts, lady's-grass 1-3 parts, 2-4 parts of concave head bamboo water pipe, chicken gizzard 10-20 parts, duck intestines 10-20 Part, shrimp med 4-6 part, hippocampus 1-3 part, bone meal 8-10 parts, water 5-15 parts, it is nutritious, addition Chinese herbal medicine, raising catfish it is disease-resistant Ability.The B of Chinese patent CN 103892111 disclose one kind and replenish the calcium catfish feed, are made from the following raw materials in parts by weight：Corn Face 20-30, peanut cake 20-25, buckwheat shell 15-20, cushaw stem 4-5, day coriander 6-9, sheep bone 7-10, duck intestines 4-5, adhesive rehmannia flower 2- 3rd, iron amaranth 1-2, White Chloe 1-2, salt 2-3, lanolin 1-2, earthworm 12-16, vinegar grain 10-12, pork liver powder 5-6, auxiliary agent 3-5, water In right amount；The fish meal is formulated from plurality of raw materials component, rich in protein, multivitamin, mineral matter and micro unit Element, makes the nutrition of feed more comprehensive, meets the nutritional need in catfish growth course, can effectively improve the survival rate of catfish And premunition, environmental pollution is reduced, achieve good economic and social benefit.The feed of the invention simultaneously can improve catfish Absorption to nutritional ingredient in feed, while increasing the palatability of feed, improves the utilization rate of feed.Though patent disclosed above Right nutrition-allocated proportion balance, but palatability, feed digestion utilization rate are still undesirable.

In order to solve the problems, such as to cause palatability poor because plant feed ratio is raised, correspondingly occur in that some are lured Food agent：The B of Chinese patent CN 103053842 disclose a kind of catfish food calling nutritional agents, and its composition includes fresh fish slurry, spiral shell freshwater mussel meat Slurry, earthworm powder, yellow meal worm powder, spirulina powder, dusty yeast, pork liver xeraphium, EM bacterium, wheat-middlings, wheat-middlings are matrix, and various solids are former Material is crushed respectively, and trash fish is milled to fish slurry, and spiral shell freshwater mussel is shelled and takes meat and wear into meat slurry, and various raw material powder, slurry are with 100 mesh sieve tulles mistakes Sieve, is put into fodder mixing machine according to proportioning and stirs 30-50 minutes, puddles uniform, is stored after air-drying, when using by 15%- 25% ratio is made an addition in catfish feed；Invention formula is from various fresh animal raw materials, animal powder, spirulina, yeast And microorganism formulation, smell is unique, attractant is strong, and for the conversion feed of catfish, catfish domestication food calling newly puts pond Nian in a suitable place to breed The feedstuff feeding of fish has good attractant effect, and can also provide high-quality animal protein and various nutritional ingredients for catfish.In A kind of catfish baits of A of state patent CN 104430233 and preparation method thereof.Specifically include：The bait is by catfish feed, animal Internal organ, sucrose, the combination of sheep blood are simultaneously formed by fermentation.The raw material that the invention is used easily is obtained, and cheap, is prepared Bait out has one special fragrance, can be good at attracting catfish to take bait, then reaches the purpose risen to the bait.Meanwhile, The prepared bait low cost out of the invention, application effect significantly, is worth promoting.Though patent disclosed above can be solved Palatability problems, but can only be used as feed addictive, however it remains feed coefficient is big, feed digestion utilization rate is low, cost Problem higher.

Although catfish feed species disclosed above is various, various informative, dispensing is complicated, and catfish is not taken into full account Digestion characteristics, it is impossible to improve the digestibility of catfish feed well, not only increase aquaculture cost, and be greatly reduced The production performance of catfish, reproductive performance and anti-disease performance, overall economic efficiency are still undesirable, catfish and other fish one Sample, catfish is used as a kind of animal feeding habits fingerling, it is not necessary to maintain constant body temperature, and energy needed for growth is low, and can ingest natural bait Material, by the gill, skin absorption inorganic salts, and easily excludes waste, causes man-made feeds to save mineral matter relatively；Catfish disappears Change that Organ Differentiation is simple and short, glandula digestive undeveloped ability more small than terrestrial animal that the ratio between alimentary canal is long with body, it is impossible to Eccrine fiber element enzyme, can not digest crude fibre substantially；Protease, amylase secretion are not enough, and protein, carbohydrate are utilized Ability；Although strong to fat utilization ability, unrighted acid is mainly needed；It is high to protein requirements, calcium phosphorus ration 1: 1-1.5, vitamin (B family vitamin especially B₁, B₂, vitamin C, vitamin A) requirement is big etc., relative to animal and fowl fodder Compounding is more complicated.The A of Chinese patent CN 104543450 disclose a kind of fermentation catfish feed, by the raw material of following weight portion It is made：Soy noodle 80-90, sunflower meal 20-25, corn protein powder 6-8, red date powder 10-14, oyster meat 10-15, squid chip 5- 10th, fermented bean curd 13-17, peach kernel 5-7, corn stem leaf 10-12, lemon 13-16, flower of Panax ginseng 1-2, palma 2-3, leek 2-3, Yolk powder 7-9, butter 3-4, saccharomycete 2-3, phagostimulant 3-4, appropriate amount of water；The fermentation catfish feed of the invention, the oyster of addition Meat, squid chip are rich in multiple nutritional components such as the several mineral materials such as protein, calcium.The lemon of addition can play antibacterial, heat-clearing, Orectic effect.Obtained catfish feed is rich in various protein, fat, vitamin and mineral matter needed for catfish, and proportioning is closed It is suitable, accelerate the growth of catfish, improve immunity of organisms and tissue metabolism's ability.Saccharomycete feature disclosed above is poor, point The enzyme system secreted is single, and feed digestion utilization rate is still undesirable.

Functional feed complex enzyme is added in catfish feed to be expected to thoroughly to solve the above problems, and mainly has following four Reason：1) degraded ANFs present in catfish feed.These materials can not be degraded by catfish endogenous enzymes, so as to disturb The eubolism of catfish, causes catfish indigestion, production performance to decline.2) utilization of starch, protein and mineral matter is improved Rate.These materials are surrounded by rich fibrous cell membrane, or are existed with some structure types that can not be digested by catfish (such as in plant feed raw material, substantial amounts of phosphorus exists in the form of phytate phosphorus.).3) degrade in the feed some specificization Learn key.These chemical bonds can not be degraded by the enzyme of catfish itself, and more nutrients can be discharged after addition exogenous enzymes.4) Because young age catfish own digestive system is also immature, endogenous enzymes are not enough, and addition exogenous enzymes can improve feed digestibility, prevent There is indigestion symptom.In addition to the utilization rate that can improve daily ration, the enzyme-added difference that can also be reduced between feedstuff It is different, the accuracy of feed formula is improved, while the regularity of catfish growth can also be improved, management cost is reduced, improve economical Benefit.Can be with environmental protection using enzyme preparation.Because the utilization rate of feed improves, corresponding excrement discharge capacity have dropped. In the case where effect is obvious, the discharge capacity of excrement can reduce 20% or so.For phytate phosphorus, can be greatly reduced Pollution of the phosphorus to water body environment.

Therefore, animal plant feedstuff science is compounded, with reference to factors such as catfish digestion characteristics, nutritional need and water body environments, Addition feed complex enzyme, prepare that a kind of feed coefficient is low, digestibility is high, production performance good, reproductive performance is high, disease-resistant performance is strong, Palatability is strong, the catfish feed of low cost is necessary.

The content of the invention

Technical problem solved by the invention is the defect for overcoming existing catfish feed, with catfish nutritional need, water body ring Based on border, with reference to its digestion characteristics, optimize existing catfish feed formula, science compounding can effectively degrade anti-in catfish feed Trophic factors, promotes the utilization rate of the nutriments such as starch, protein and mineral matter, improves protein level, the body of catfish Antioxidation activity and digestibility, significantly improve catfish digestibility and production performance, reduce feed coefficient, and anti-water-stop body is dirty Dye, the feed complex enzyme for maintaining fine water；Catfish feed caking property, water resistance, glossiness and frost resistance are remarkably improved, are dropped Wormwood seed powder of the low feed containing powder rate；The calcium berry extract of calcium, iron and Soluble Fiber can effectively be supplemented；Feed can be greatly improved The phagostimulant of palatability；Finally prepare that a kind of feed coefficient is low, digestibility is high, production performance good, reproductive performance is high, disease-resistant performance By force, palatability is strong, the catfish feed of low cost.

In order to achieve the above object, the present invention uses following technical scheme：

A kind of digestive utilization ratio catfish feed high, is mainly prepared by the raw material of following parts by weight：

Corn 40-60 parts, wheat bran 30-50 parts, chicken gizzard 20-40 parts, 20-40 parts of the flax dregs of rice, 10-30 parts of trash fish powder, clover 10-30 parts, 8-12 parts of calcium berry extract, phagostimulant 6-10 parts, 2-6 parts of wormwood seed powder, feed complex enzyme 2-6 parts, EM bacteria powders 0.5-1.5 parts, salt 0.6-1.2 parts, appropriate amount of water；

Preferably, the digestive utilization ratio catfish feed high, is mainly prepared by the raw material of following parts by weight：

Corn 45-55 parts, wheat bran 35-45 parts, chicken gizzard 25-35 parts, 25-35 parts of the flax dregs of rice, 15-25 parts of trash fish powder, clover 15-25 parts, 9-11 parts of calcium berry extract, phagostimulant 7-9 parts, 3-5 parts of wormwood seed powder, feed complex enzyme 3-5 parts, EM bacteria powders 0.8-1.2 parts, salt 0.8-1.0 parts, appropriate amount of water；

It is highly preferred that the digestive utilization ratio catfish feed high, is mainly prepared by the raw material of following parts by weight：

50 parts of corn, 40 parts of wheat bran, 30 parts of chicken gizzard, 30 parts of the flax dregs of rice, 20 parts of trash fish powder, 20 parts of clover, calcium berry extract 10 parts, 8 parts of phagostimulant, 4 parts of wormwood seed powder, 4 parts of feed complex enzyme, 1 part of EM bacteria powders, 0.9 part of salt, appropriate amount of water；

Further, the feed complex enzyme is uniformly mixed by the raw material of following parts by weight：Yeast culture 20- 40 parts, Wheat Sprout Powder 20-30 parts, acidic xylanase 20-30 parts, acid protease 15-25 parts, phytase 15-25 parts, β-Portugal Dextranase 10-20 parts, cellulase 10-15 parts, amylase 10-15 parts, 'beta '-mannase 10-15 parts, pectase 5-10 parts；

The acidic xylanase, acid protease, phytase, 1,4 beta-glucanase, cellulase, amylase, β-sweet dew Dextranase, pectase are food-grade enzyme preparation；

Preferably, the yeast culture is to be with saccharomyces cerevisiae (Saccharomyces cerevisiae) tlj2016 The bacterium that sets out prepares through spreading cultivation step by step, fermenting；

It is highly preferred that the preparation method of the yeast culture, comprises the following steps：Take the saccharomyces cerevisiae after the activation of 1 ring Tlj2016 slant strains, be seeded in the 500m L triangular flasks equipped with 100m L seed culture mediums cultivate, 28~30 DEG C, shake 25~30h is cultivated under the conditions of 150~200r/min of bed rotating speed, primary seed solution is obtained；Primary seed solution is pressed into 5~10% (v/ V) inoculum concentration, being seeded in the 500m L triangular flasks equipped with 100m L fermentation mediums carries out fermented and cultured, fermentation time 25 ~30h, 28~30 DEG C of temperature, 150~200r/min of shaking speed obtains secondary seed solution；By secondary seed solution with 5~10% (v/v) inoculum concentration is linked into the 5L fermentation tank ferment at constant temperature of dress 3L fermentation mediums, 28~30 DEG C of temperature, 4~6L/ of ventilation Min, 150~200r/min of rotating speed, fermentation full adjustment zymotic fluid pH value are 6.0~6.5, when fermentation is to 25~30h, with every The addition of 25~30mmol of zymotic fluid is risen to disposable addition Cys in zymotic fluid, is continued fermentation 20-30h and is obtained most Whole zymotic fluid；Final zymotic fluid is concentrated under reduced pressure into solid content for 18-26%, and freeze-drying obtains final product yeast culture；

GSH final concentrations reach 3308mg/L in the final zymotic fluid；

Viable bacteria content is in the yeast culture：7×10¹²-9×10¹²cfu/g；

The quality group of the seed culture medium turns into：(NH₄)₂SO₄6g/L, glucose 35g/L, K₂HPO₄·3H₂O 3g/ L, KH₂PO₄0.5g/L, dusty yeast 11g/L, MnSO₄0.1g/L, KCL 0.1g/L, FeSO₄0.1g/L, MgSO₄·7H₂O 0.1g/L, balance of water, pH 6.0；

The quality group of the fermentation medium turns into：(NH₄)₂SO₄10g/L, molasses 150g/L, K₂HPO₄·3H₂O 8g/ L, KH₂PO₄0.5g/L, dusty yeast 5g/L, corn pulp 10g/L, MnSO₄0.1g/L, KCL 0.1g/L, FeSO₄0.1g/L, MgSO₄·7H₂O 0.1g/L, balance of water, pH 6.0.

The saccharomyces cerevisiae is specially saccharomyces cerevisiae (Saccharomyces cerevisiae) tlj2016, and the bacterial strain is China Committee for Culture Collection of Microorganisms's common micro-organisms center is preserved on July 15th, 2016, deposit number is CGMCC No.12789, preservation address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica, Postcode 100101；

The saccharomyces cerevisiae tlj2016 has following characteristic：1) tolerance to glucose reaches 300g/L, beneficial to it GSH is produced under the conditions of high concentration glucose；2) 3308mg/L is reached in 5L fermentation cylinder for fermentation production GSH final concentrations；3) it is resistance to By the ability extra-heavy of Cys, slow growth is remained under the effect of 5mmol/LL- cysteines, in 40mmol/L L- half Remain to keep GSH largely to synthesize under cystine effect；4) salt resistance ability reaches 18%, is conducive to extending its application field.

Further, the preparation method of the Wheat Sprout Powder, comprises the following steps：By wheat be placed on temperature for 10-18 DEG C, To soak 20-40min in the soak of 6-8, soak contains the sodium acid carbonate that mass percent concentration is 0.1-0.3% to pH value With the compound enzyme of 0.3-0.5%, 28-32 DEG C then is warming up to the speed of 3-5 DEG C/min, continues to soak 3-5h, finally with 5-7 DEG C/speed of min is warming up to 50-60 DEG C of immersion 5-8min, whole immersion process is divulged information once every 15-20min, ventilation pressure 0.13-0.15MPa, while in electric-field intensity 3-5kV/cm, burst length 100-300 μ s, pulse frequency 60-80Hz conditions are carried out High-pressure pulse electric treatment, until wheat water content content is 40-45%；Wheat after immersion is drained, and is carried out in growing floor dark Germination, dark germination temperature is kept for 20-24 DEG C, and the dark germination time is 20-24h, is through the wheat drying after germination to moisture 5-8%, low-temperature grinding crosses 60-80 mesh sieves and obtains final product Wheat Sprout Powder；

The compound enzyme is cellulase, 1,4 beta-glucanase, zytase 3-5 in mass ratio:2-4:1-3 uniformly mixes.

Further, the calcium berry extract is with calcium, iron and dietary fiber content calcium stem end higher, leaf of GAIGUO and chrysanthemum Powder is raw material, through ultrasonic cleaning, biology enzyme enzymolysis, microwave drying, extrusion, ultramicro grinding and be obtained；Its Soluble Fiber Cellulose content is high, bioactivity is strong, have important, positive role fiber to beneficial flora in catfish body, can particularly supplement calcium The shelf-life of iron, extension feed, compared with full diet fiber, its feature, bioactivity are more powerful；

Preferably, the preparation method of the calcium berry extract, comprises the following steps：Calcium stem end, leaf of GAIGUO and inulin are pressed Mass ratio 5-9:3-6:1-3 uniformly mixes, and is placed in supersonic wave cleaning machine and cleans 3-5min in 200W, 30KHz, drains, and crushes To particle diameter 0.3-0.7mm, the water of 0.5-1.5 times of fragment quality is added, regulation pH value is 5-7, adds mixture quality 0.1- 0.3% biology enzyme, 10-15min is digested in 50-60 DEG C；Enzymolysis liquid is concentrated under reduced pressure into solid content for 40-60%, is put into Microwave dryer carries out intermittent drying in 2000W, 130-150 DEG C, is allowed to moisture and reaches 4-6%, is then crushed to particle diameter 0.4-0.6mm, adds the sodium acid carbonate of crushed material quality 0.2-0.4%, and uniform mixing, adjustment mixture moisture is 15- 18%, room temperature, sealing and standing 2-4h, in screw speed 105-115r/min, 140-160 DEG C of condition extrusion of temperature, then Ultramicro grinding obtains final product calcium berry extract to particle diameter 0.1-0.3mm；

The biology enzyme is cellulase, laccase, dextranase, seminase, pectase, tannase 2-4 in mass ratio: 2-4:1-3:1-3:0.5-1.5:0.4-1 uniformly mixes.

Further, the phagostimulant is science compounding sheep blood and earthworm, through high-pressure pulse electric, complex enzyme segmentation enzyme Solution, ultrafiltration, segmentation reaction, the low temperature processing techniques such as freeze-drying and prepare have sheep blood concurrently and the composite flavor of earthworm local flavor is in Gustin, is not only greatly improved the palatability of catfish feed, improves efficiency of feed utilization, and with compared with strong anti-oxidation effect, disappear Except free radical；

Preferably, the preparation method of the phagostimulant, comprises the following steps：1-3 in mass ratio:2-8 weighs fresh sheep blood And earthworm, mix, rub to obtain compound, the solion of 2-5 times of its quality is added, mix, regulation pH value is 6-8, in room temperature Under in electric-field intensity 25-35kV/cm, burst length 300-500 μ s, high-voltage pulse is carried out under the conditions of pulse frequency 200-300Hz Electric field treatment 20-30min；The complex enzyme of mixture quality 0.04-0.08% is subsequently adding, 30-50min is digested in 50-60 DEG C, Then 35-45 DEG C of enzymolysis 40-60min, 90 DEG C of enzyme 10min that go out are cooled to, room temperature is down to, aseptic filtration obtains enzymolysis liquid, enzymolysis liquid By the ultrafiltration membrance filter of 1000Da and 5000Da, the permeate between two films is taken, be concentrated under reduced pressure into solid content for 18- 22% must digest concentrate；The reduced sugar of enzymolysis concentrate quality 2-4% is added, 30-50min is reacted in 112-118 DEG C successively After 135-145 DEG C of reaction 45-65min；By reaction solution by the ultrafiltration membrance filter of 1000Da and 5000Da, take between two films Permeate, is concentrated under reduced pressure into solid content for 26-30% must react concentrate；To reaction concentrate in add its quality 0.5- 1.5% sericin peptide taken, mixes, and freeze-drying obtains final product phagostimulant.

Preferably, the solion is to contain sodium ion 17-22mg/L, zinc ion 15-20mg/L, potassium ion 12- The aqueous solution of 14mg/L, calcium ion 9-11mg/L and magnesium ion 6-10mg/L；

Preferably, the complex enzyme is earthworm serine protease, alkali protease, flavor protease, lipase by matter Amount compares 4-6:2-4:0.2-0.4:0.1-0.3 uniformly mixes；

Preferably, the reduced sugar is one or more in xylose, ribose, glucose, fructose, galactolipin；

It is highly preferred that the quality group of the reduced sugar turns into：Xylose:Ribose:Glucose:Fructose:Galactolipin=3-5:2- 4:1-3:1-3:1-2。

Further, the wormwood seed powder is with wormwood seed as raw material, through high-pressure electrostatic, high-pressure pulse electric auxiliary bigcatkin willow Crushed after acid soak, refrigerating treatment, freeze and be obtained；

Preferably, the preparation method of the wormwood seed powder, comprises the following steps：By wormwood seed sabot, first in electric-field strength Degree 1-3kV/cm high-pressure electrostatic treatment 8-10min；Then concentration for 6-8mg/L salicylic acid solution in soaking at room temperature 2-5h, Simultaneously in electric-field intensity 2-6kV/cm, burst length 50-100 μ s, high-voltage pulse electric is carried out under the conditions of pulse frequency 100-150Hz Field treatment；Rinse, drain, in 3-5 DEG C stand 18-24h, then successively 1-3 DEG C refrigerate 2-4d, -3--5 DEG C freezing 1-3d, - 15--18 DEG C of freezing 10-15h, is immediately placed at outdoor natural light according to being crushed immediately after 2-4h, freezes, and adds freeze-dried powder quality The sodium bicarbonate of 3-8%, uniform mixing is crossed 80-100 mesh sieves and obtains final product wormwood seed powder.

Another object of the present invention is to provide the preparation method of above-mentioned digestive utilization ratio catfish feed high, comprises the following steps：

1) according to formula precise each component raw material, corn, wheat bran, oil cake of flax seed, clover are respectively dried to moisture and are contained It is 8-12% to measure, and is crushed, and crosses 60-80 mesh sieves, then uniformly mixes to obtain mixed powder A with trash fish powder, phagostimulant, wormwood seed powder；

2) chicken gizzard is cleaned, is cut into slices, 1-3h is soaked in mass percent concentration is for the silk gum peptide solution of 0.8-1.4%, Take out, freeze, crush to obtain chicken liver meal；

3) 30-50% of calcium berry extract quality is taken, successively EM bacteria powders, feed complex enzyme, chicken liver meal is added thereto, Uniformly mix to obtain mixed powder B；

4) remaining calcium berry extract and salt are mixed, adds suitable quantity of water, it is 15-25%'s to be made mass percent concentration The aqueous solution；

5) stirred in mixed powder B, mixed powder A the addition aqueous solution successively, granulation, drying digestion high Utilization rate catfish feed.

Beneficial effect：

Present invention digestive utilization ratio catfish feed high is special with reference to its digestion based on catfish nutritional need, water body environment Property, optimizing existing catfish feed formula, science compounds the ANFs in the catfish feed that can effectively degrade, and promotes starch, egg The utilization rate of the nutriment such as white matter and mineral matter, improves the protein level of catfish, body antioxidation activity and digests and assimilates Rate, significantly improves catfish digestibility and production performance, reduces feed coefficient, prevents water pollution, maintains the feed of fine water Complex enzyme；Catfish feed caking property, water resistance, glossiness and frost resistance are remarkably improved, wormwood seed of the feed containing powder rate is reduced Powder；The calcium berry extract of calcium, iron and Soluble Fiber can effectively be supplemented；The phagostimulant of feed palatability can be greatly improved；Finally Prepare that a kind of feed coefficient is low, digestibility is high, production performance good, reproductive performance is high, disease-resistant performance is strong, palatability is strong, low cost Catfish feed.1) feeding experiment shows：With same feeding and acclimation method, same daily management, same fish pond bar Catfish, digestive utilization ratio catfish feed high prepared by the present invention are fed when part and same native catfish fingerling and injected volume Palatability is strong, and adult fish specification gap is small, and neatly, and then catfish baits, competes the situation of looking for food slightly, and it is only 4% that the death rate is low, than The commercially available digestibility catfish feed reduction by 63.63% high of control group；Disease resistance is strong, and the incidence of disease is only 3%, more commercially available than control group high to disappear Rate catfish feed reduction by 66.67%；Digestibility is high, and tail increases weight by force, and digestibility catfish feed high more commercially available than control group is improved 55.74%；Feed coefficient is low, and feedstuff-meat ratio is low, digestibility catfish feed reduction by 35.29% high more commercially available than control group；Culture benefit Significantly, output increased 55.88% in the case of same injected volume, greatly reduces aquaculture cost, with preferable practicality and elder generation The property entered.2) stability test shows：Under the conditions of -12 DEG C and 40 DEG C store 12 months, catfish feed of the present invention compared with commercially available, The loss of its proteinase activity reduces by 40% and 18.2% respectively, and with excellent temperature storage, enzyme activity is highly stable, while Also illustrate that the loss late of other bioactivators that catfish feed of the present invention contains is extremely low, with preferable freeze proof, heat resistance Can, product quality stabilization, long shelf-life.3) detected through quality index：Compared with commercially available catfish feed, catfish feed water of the present invention Point content and water activity reduce by 37.36% and 37.38% respectively, not easy infection miscellaneous bacteria, long shelf-life, good stability；Containing powder Rate reduction by 66.67%, efficiency of feed utilization is high, loses small, is conducive to maintaining excellent water quality environment；Pulverization rate reduction by 82.61%, Feed caking property is high, and particle is neat, and glossiness is strong, is difficult, in transport and storage process friction efflorescence, to improve raw material availability, Promote the palatability of feed；Hardness improves 31.9%, and water resistance and durability degree are strong, are conducive to catfish to look for food, and extension is looked for food Time, improve efficiency of feed utilization and digestibility；Gelatinization degree is high, improves 83.3%, is easy to catfish to digest, absorb, significantly Improve the digestive utilization ratio of catfish.Particular technique effect is shown in embodiment 7-10；Particular technique principle is as follows：

1. the feed complex enzyme that the present invention is provided is by the Wheat Sprout Powder containing abundant natural plants enzyme system and species, existing feeding Material enzyme preparation and feature yeast culture science compounding are formed, and the ANFs in the catfish feed that can effectively degrade promotes The utilization rate of the nutriments such as starch, protein and mineral matter, improves the protein level of catfish, body antioxidation activity and disappears Change absorptivity, significantly improve catfish digestibility and production performance, reduce feed coefficient, prevent water pollution, maintain fine water, Improve production performance, reproductive performance and the disease-resistant performance of catfish.Saccharomyces cerevisiae tlj2016 culture viable bacterias particularly therein contain Amount up to 7 × 10¹²-9×10¹²Cfu/g, GSH final concentrations reach 3308mg/L in final zymotic fluid, not only increase catfish feeding The protein level and enzymatic activity of material, and the bioactivity and inoxidizability of catfish feed are improve, greatly manifest feeding Expect the functional characteristic of complex enzyme, saccharomyces cerevisiae tlj2016 is except possessing the probiotic outer also with following functions characteristic of common yeast： 1) tolerance to glucose reaches 300g/L, and GSH is produced under the conditions of high concentration glucose beneficial to it；2) in 5L fermentation tanks Middle fermenting and producing GSH final concentrations reach 3308mg/L；3) the ability extra-heavy of Cys is tolerated, in the Guang ammonia of 5mmol/L L- half Slow growth is remained under acid effect, remains to keep GSH largely to synthesize under the effect of 40mmol/L Cys；4) salt tolerant energy Power reaches 18%；Its science is compounded can be further in catfish feed：(1) free radical, peroxide, heavy metal and Huang are removed The poisonous substances such as aspertoxin；(2) transhipment of amino acid (glutaminase, cysteine and other neutral amino acids) is participated in；(3) it is beneficial to The absorption of iron, the absorption of selenium, the absorption of calcium, glutathione can also make the peroxidating aliphatic acid in feed when absorbing or absorption After revert to normal fat；(4) gastrointestinal tract mucosa epithelium is protected, is prevented because inflammation, ischaemic, oxidation material etc. are glutinous to intestines The damage of film；(5) store and its is provided and constitute amino acid (especially cysteine)；(6) synthesis of protein and DNA is participated in； (7) as reducing substances, beneficial to vitamin E, ascorbic reduction, sulfydryl enzymatic activity is maintained.

2. the wormwood seed powder that prepared by the present invention, with stronger caking property, is fitted very much while abundant nutrition material is provided Close and prepare catfish pellet with other animal-plant materials of the invention and auxiliary agent, be greatly improved pellet into graininess and complete Whole property, the catfish feed water-tolerant of preparation, glossiness is strong, and rate containing powder is low, and efficiency of feed utilization is high；Wormwood cold resistance is strong simultaneously, (wormwood seed) wormwood seed is rich in antifreeze protein, through high-pressure electrostatic treatment, high-pressure pulse electric auxiliary Induced by Salicylic Acid, low temperature point Section Stress treatment and natural lighting organically combine so that itself containing freeze proof matrix active seed outer boundary's environment stress and Under induction, freeze proof matrix components have obtained most comprehensive, most abundant synthesis and accumulation, improve the content of antifreeze protein, and then The cryoprotective effects and stability of nutrition and bioactivator in catfish feed are improve, the shelf-life of feed is extended.With calcium Other raw material science compounding such as berry extract, phagostimulant, it is better.

3. the calcium berry extract that prepared by the present invention is with calcic, iron and dietary fiber content calcium stem end, leaf of GAIGUO higher With inulin be raw material, through ultrasonic cleaning, biology enzyme enzymolysis, microwave drying, extrusion, ultramicro grinding and be obtained；It is soluble Content of cellulose is high, bioactivity is strong, have important, positive role fiber to beneficial flora in catfish body, can particularly mend Calcium iron, the shelf-life of extension feed are filled, compared with full diet fiber, its feature, bioactivity are more powerful；Simultaneously through ultra micro After crushing, add a certain amount of sodium acid carbonate, sealing and standing after adjusted moisture, can significantly increase dietary fiber puffed degree and The content of soluble dietary fiber, improves the feature of dietary fiber, and gained calcium berry extract thickening property is stronger, than single side Modified dietary fiber prepared by method improves 40-50%, and is not influenceed by acid, alkali, salt, significantly improves the water resistance of feed； Soluble Fiber cellulose content is high, and 20-40% is improved than modified dietary fiber prepared by single method, it is easier to by lactic acid bacteria profit With, growth and fertility of the lactic acid bacteria in animal body enteron aisle are improved, increase the type and quantity of probiotics flora, reduce animal Body gut pH, improves animal body intestine microenvironment；High adsorption capacity, after modified, the specific surface area increase of cellulose, Network enrich, absorption affinity enhancing, chelating, absorption cholesterol and bile acids organic molecule ability it is stronger, suppress animal Absorption of the body to them；Ion-exchange capacity strengthens, and to metallic element, particularly heavy metal element adsorption effect is stronger, effectively Prevent the weight of animals metal poisoning；Regulation and the resident time of maintenance gut flora, strengthen digestion and the absorbability of enteron aisle, Improve immunity of organisms；Gastrointestinal peristalsis is effectively facilitated, slows down and eliminate the adverse reactions such as gasteremphraxis, abdominal distension；Powerful embedding effect Environment (oxygen, temperature, the illumination, water activity etc.) influence of factor to feeding quality can be prevented, feed is further stabilized Bioactivity and stability, extend the shelf-life of product.

4. the phagostimulant that prepared by the present invention is science compounding sheep blood and earthworm, through high-pressure pulse electric, complex enzyme segmentation enzyme Solution, ultrafiltration, segmentation reaction, the low temperature processing techniques such as freeze-drying and prepare have sheep blood concurrently and the composite flavor of earthworm local flavor is in Gustin, taste compound enriches, and is particularly suitable for catfish taste, can not only improve the palatability of catfish feed, but also can be complete Face promotes the digestion and absorption of catfish feed nutrient, improves efficiency of feed utilization, organic in excreta so as to greatly reduce The discharge rate of the nutriments such as thing, nitrogen phosphorus, reduces pollution of the undigested material to water environment in feed.Whole process uses low temperature process Technology, it is to avoid because high temperature and caused by albuminous degeneration and other bioactivators and fragrance matter lose, using high-tension pulse Electric field technology is rushed, extraction process living contaminants can be not only prevented, and it is significantly loose between sheep blood and earthworm protein and tissue Structure, increased the permeability of tissue, be conducive to the infiltration and contact of follow-up complex enzyme, improve enzymolysis efficiency and flavor peptides Recovery rate, while also can directly increase the dissolution of soluble flavor peptides, pre-processes more efficient, more environmentally friendly relative to existing high temperature； Using subsection enzymolysis and segmentation Maillard reaction, the yield of composite flavor phagostimulant is further increased；Solion and compound The science compounding of enzyme further increases the activity of complex enzyme, improves enzymolysis efficiency, reduces the usage amount of enzyme preparation, maximum Improve to limit the acquisition of composite flavor peptide；Using freeze-drying, especially compounded with excellent freeze proof to reaction solution science The sericin peptide taken of effect, the oxidational losses and freezing for greatly reducing phagostimulant is lost, and improves the inoxidizability of phagostimulant, finally A kind of palatability for being not only greatly improved catfish feed is obtained, efficiency of feed utilization is improved, and with compared with strong anti-oxidation effect, Eliminate the phagostimulant of free radical.

5. the Wheat Sprout Powder that prepared by the present invention carries out high-pressure pulse electric treatment to the wheat of immersion process and effectively prevent Soak pollution microbes, it is to avoid generation stink is penetrated into Wheat Sprout Powder product, meanwhile, can effectively strengthen cereal kind chrotoplast The relative permeability of wall and cell membrane, the water absorption rate for improving full cereal seed, promote seed to sprout in advance, improve superoxide anion certainly By generation speed and atriphos (ATP) content of base, the activation of the various biology enzymes of stratification early stage wheat seed and release Put, endosperm dissolves and functional nutrient composition, bioactive ingredients, the synthesis of antioxidant content, promotes the breathing generation of seed The effect of thanking, accelerates nutrition and functional materials, bioactive ingredients, the enrichment process of antioxidant content, when shortening enrichment Between, nutrition and functional materials, bioactive ingredients, the content of antioxidant content are improve, soaked with segmentation, rapidly heated up, Biological enzymolysis technique is organically combined, and can further degrade kind of hide fiber element and a hemicellulose structure, increase kind of a skin permeability, activation Various bioactivator (endogenous enzymes etc.) vigor are most strong, and enriching quantity is bigger, and Soluble Fiber cellulose content increases therewith, is wheat Catfish feed in bud powder provides trophic factors, better notable；By segmentation immersion, high-voltage pulse electric field technology and biology Enzymolysis is organically combined, and shortens soak time, improves germination percentage and germination uniformity；Wheat Sprout Powder heat can to greatest extent be kept Quick property content of material, especially polyphenoils (glutathione, IP6, vitamin C, polyphenol etc.), to greatest extent Ensure natural colored, the taste and flavor of Wheat Sprout Powder, while may also function as bactericidal action；Particularly moderately killed through high temperature, short time After embryo, in the case where the bioactivity of wheat malt seed is not influenceed, with the absorption of moisture, wheat embryo is suppressed but not shadow The growth and the dissolving of endosperm of items enzyme and bioactivator are rung, bud growth is very short, and respiration is weak, and sprout damage is low, carries The high yield and quality of Wheat Sprout Powder, significantly improves the functional characteristic and digestion characteristics of catfish feed.

6. the preparation method process is simple of catfish feed of the present invention, low for equipment requirements, by with powerful embedding effect Calcium berry extract is prepared into softwood；Chicken gizzard is soaked in silk gum peptide solution, fully freeze-drying after infiltration, in can preventing chicken gizzard Topmost vitamin E etc. is anti-oxidant to be lost with active material high temperature or cryogenic freezing, improves the content of chicken gizzard active principle； EM bacteria powders, feed complex enzyme, chicken liver meal that bioactive substance content enriches are embedded in calcium berry extract first, then Mix with other raw materials, in sequentially adding the softwood of preparation, can to greatest extent reduce catfish feed in granulation, storage Nutrition and the loss of active material, improve trophism, bioactivity and the quality stability of catfish feed, effectively extend feed Shelf-life, improve the foodsafety of catfish.

It should be noted that digestive utilization ratio catfish feed high of the invention has the technical effect that each component technical characteristic and side The result that method feature is mutually cooperateed with, interacted, the superposition of not simple technical characteristic (component function), each component technology is special The effect that the combination levied and collaboration are produced considerably beyond each monotechnics feature functionality and effect superposition, with preferable Advanced and practicality.

Specific embodiment

The present invention is described below by specific embodiment.Unless stated otherwise, technological means used in the present invention It is method known in those skilled in the art.In addition, embodiment is interpreted as illustrative, it is not intended to limit the present invention Scope, the spirit and scope of the invention are limited only by the claims that follow.To those skilled in the art, without departing substantially from this On the premise of invention spirit and scope, the various changes that are carried out to the material component and consumption in these embodiments or change Belong to protection scope of the present invention.

It is prepared by the raw material of embodiment 1

1. the preparation of yeast culture

The preparation method of the yeast culture, comprises the following steps：The saccharomyces cerevisiae tlj2016 taken after the activation of 1 ring is oblique Face strain, is seeded in the 500m L triangular flasks equipped with 100m L seed culture mediums and cultivates, in 30 DEG C, shaking speed 150r/min Under the conditions of cultivate 28h, obtain primary seed solution；By primary seed solution by the inoculum concentration of 8% (v/v), it is seeded to equipped with 100m L Carry out fermented and cultured in the 500m L triangular flasks of fermentation medium, fermentation time 28h, 30 DEG C of temperature, shaking speed 180r/min, Obtain secondary seed solution；Secondary seed solution is linked into the 5L fermentation tank constant temperature of dress 3L fermentation mediums with 8% (v/v) inoculum concentration Fermentation, 30 DEG C of temperature, ventilation 5L/min, rotating speed 200r/min, fermentation full adjustment zymotic fluid pH value is 6.0~6.5, works as hair When ferment is to 30h, with every liter of addition of zymotic fluid 28mmol to disposable addition Cys in zymotic fluid, continue to ferment 25h obtains final zymotic fluid；It is 22% that final zymotic fluid is concentrated under reduced pressure into solid content, and freeze-drying obtains final product Yeast Cultivation Thing；

GSH final concentrations reach 3308mg/L in the final zymotic fluid；

Viable bacteria content is in the yeast culture：9×10¹²cfu/g；

2. the preparation of Wheat Sprout Powder

The preparation method of the Wheat Sprout Powder, comprises the following steps：Wheat is placed on the leaching that temperature is 15 DEG C, pH value is 7 30min is soaked in bubble liquid, soak contains the sodium acid carbonate and 0.4% compound enzyme that mass percent concentration is 0.2%, then 30 DEG C are warming up to the speed of 4 DEG C/min, continue to soak 4h, being finally warming up to 55 DEG C with the speed of 6 DEG C/min soaks 7min, whole Individual immersion process is divulged information once every 18min, ventilation pressure 0.14MPa, while in electric-field intensity 4kV/cm, the μ of burst length 200 S, pulse frequency 70Hz condition carry out high-pressure pulse electric treatment, until wheat water content content is 42%；Wheat drip after immersion It is dry, dark germination is carried out in growing floor, dark germination temperature is kept for 22 DEG C, and the dark germination time is 22h, through the wheat drying after germination It is 6% to moisture, low-temperature grinding crosses 80 mesh sieves and obtains final product Wheat Sprout Powder；

The compound enzyme is cellulase, 1,4 beta-glucanase, zytase in mass ratio 4:3:2 uniform mixing.

3. the preparation of calcium berry extract

The preparation method of the calcium berry extract, comprises the following steps：By calcium stem end, leaf of GAIGUO and inulin in mass ratio 7: 5:2 uniform mixing, are placed in supersonic wave cleaning machine and clean 4min in 200W, 30KHz, drain, and are crushed to particle diameter 0.5mm, add The water that 1 times of fragment quality, regulation pH value is 6, adds the biology enzyme of mixture quality 0.2%, and 12min is digested in 55 DEG C；Enzyme It is 50% that solution liquid is concentrated under reduced pressure into solid content, and be put into microwave dryer carries out intermittent drying in 2000W, 140 DEG C, is allowed to Moisture reaches 5%, is then crushed to particle diameter 0.5mm, adds the sodium acid carbonate of crushed material quality 0.3%, and uniform mixing, adjustment is mixed Compound moisture is 16%, room temperature, sealing and standing 3h, in screw speed 110r/min, 150 DEG C of condition extrusions of temperature, Then ultramicro grinding obtains final product calcium berry extract to particle diameter 0.2mm；

The biology enzyme is cellulase, laccase, dextranase, seminase, pectase, tannase in mass ratio 3:3: 2:2:1:0.7 uniform mixing.

4. the preparation of phagostimulant

The preparation method of the phagostimulant, comprises the following steps：In mass ratio 2:5 weigh fresh sheep blood and earthworm, mix, Compound is rubbed to obtain, the solion of 4 times of its quality is added, mixed, regulation pH value is 7, at room temperature in electric-field intensity 30kV/ Cm, the μ s of burst length 400, carry out high-pressure pulse electric treatment 25min under the conditions of pulse frequency 250Hz；It is subsequently adding compound The complex enzyme of quality 0.06%, 40min is digested in 55 DEG C, is then cooled to 40 DEG C of enzymolysis 50min, 90 DEG C of enzyme 10min that go out, and is down to Room temperature, aseptic filtration obtains enzymolysis liquid, and the ultrafiltration membrance filter that enzymolysis liquid passes through 1000Da and 5000Da takes the transmission between two films Liquid, is concentrated under reduced pressure into solid content for 20% must digest concentrate；The reduced sugar of enzymolysis concentrate quality 3% is added, successively In after 40min and 140 DEG C of reaction 55min of 115 DEG C of reactions；The ultrafiltration membrance filter that reaction solution is passed through into 1000Da and 5000Da, takes Permeate between two films, is concentrated under reduced pressure into solid content for 28% must react concentrate；To reaction concentrate in add it The sericin peptide taken of quality 1%, mixes, and freeze-drying obtains final product phagostimulant.

The solion is to contain sodium ion 20mg/L, zinc ion 18mg/L, potassium ion 13mg/L, calcium ion 10mg/L With the aqueous solution of magnesium ion 8mg/L；

The complex enzyme is earthworm serine protease, alkali protease, flavor protease, lipase in mass ratio 5:3: 0.3:0.2 uniform mixing；

The quality group of the reduced sugar turns into：Xylose:Ribose:Glucose:Fructose:Galactolipin=4:3:2:2:1.5.

5. the preparation of wormwood seed powder

The preparation method of the wormwood seed powder, comprises the following steps：By wormwood seed sabot, first in electric-field intensity 2kV/cm High-pressure electrostatic processes 9min；Then concentration for 7mg/L salicylic acid solution in soaking at room temperature 4h, while in electric-field intensity 4kV/ Cm, the μ s of burst length 80, carries out high-pressure pulse electric treatment under the conditions of pulse frequency 120Hz；Rinse, drain, in 4 DEG C of standings 21h, then refrigerates 3d, -4 DEG C of freezing 2d, -16 DEG C of freezing 12h at 2 DEG C successively, be immediately placed at outdoor natural light according to after 3h immediately Crushed, freezed, added the sodium bicarbonate of freeze-dried powder quality 5%, uniform mixing is crossed 80 mesh sieves and obtains final product wormwood seed powder.

Yeast culture that example 2 below -6 is used, Wheat Sprout Powder, calcium berry extract, phagostimulant, wormwood seed powder are equal For prepared by embodiment 1, other raw materials are purchased in market.

Embodiment 2

The feed complex enzyme is uniformly mixed by the raw material of following parts by weight：30 parts of yeast culture, wheat malt 25 parts of powder, 25 parts of acidic xylanase, 20 parts of acid protease, 20 parts of phytase, 15 parts of 1,4 beta-glucanase, cellulase 12 Part, 12 parts of amylase, 12 parts of 'beta '-mannase, 8 parts of pectase；

Preparation method, comprises the following steps：

1) according to formula precise each component raw material, corn, wheat bran, oil cake of flax seed, clover are respectively dried to moisture and are contained It is 10% to measure, and is crushed, and crosses 80 mesh sieves, then uniformly mixes to obtain mixed powder A with trash fish powder, phagostimulant, wormwood seed powder；

2) chicken gizzard is cleaned, is cut into slices, 2h is soaked in the silk gum peptide solution that mass percent concentration is 1.1%, taken out, frozen It is dry, crush to obtain chicken liver meal；

3) the 40% of calcium berry extract quality is taken, is successively added thereto EM bacteria powders, feed complex enzyme, chicken liver meal, It is even to mix to obtain mixed powder B；

4) remaining calcium berry extract and salt are mixed, adds suitable quantity of water, be made the water that mass percent concentration is 20% Solution；

Embodiment 3

40 parts of corn, 30 parts of wheat bran, 20 parts of chicken gizzard, 20 parts of the flax dregs of rice, 10 parts of trash fish powder, 10 parts of clover, calcium berry extract 8 Part, 6 parts of phagostimulant, 2 parts of wormwood seed powder, 2 parts of feed complex enzyme, 0.5 part of EM bacteria powders, 0.6 part of salt, appropriate amount of water；

The feed complex enzyme is uniformly mixed by the raw material of following parts by weight：20 parts of yeast culture, wheat malt 20 parts of powder, 20 parts of acidic xylanase, 15 parts of acid protease, 15 parts of phytase, 10 parts of 1,4 beta-glucanase, cellulase 10 Part, 10 parts of amylase, 10 parts of 'beta '-mannase, 5 parts of pectase；

Preparation method, comprises the following steps：

1) according to formula precise each component raw material, corn, wheat bran, oil cake of flax seed, clover are respectively dried to moisture and are contained It is 8% to measure, and is crushed, and crosses 60 mesh sieves, then uniformly mixes to obtain mixed powder A with trash fish powder, phagostimulant, wormwood seed powder；

2) chicken gizzard is cleaned, is cut into slices, 1h is soaked in the silk gum peptide solution that mass percent concentration is 0.8%, taken out, frozen It is dry, crush to obtain chicken liver meal；

3) the 30% of calcium berry extract quality is taken, is successively added thereto EM bacteria powders, feed complex enzyme, chicken liver meal, It is even to mix to obtain mixed powder B；

4) remaining calcium berry extract and salt are mixed, adds suitable quantity of water, be made the water that mass percent concentration is 15% Solution；

Embodiment 4

60 parts of corn, 50 parts of wheat bran, 40 parts of chicken gizzard, 40 parts of the flax dregs of rice, 30 parts of trash fish powder, 30 parts of clover, calcium berry extract 12 parts, 10 parts of phagostimulant, 6 parts of wormwood seed powder, 6 parts of feed complex enzyme, 1.5 parts of EM bacteria powders, 1.2 parts of salt, appropriate amount of water；

The feed complex enzyme is uniformly mixed by the raw material of following parts by weight：40 parts of yeast culture, wheat malt 30 parts of powder, 30 parts of acidic xylanase, 25 parts of acid protease, 25 parts of phytase, 20 parts of 1,4 beta-glucanase, cellulase 15 Part, 15 parts of amylase, 15 parts of 'beta '-mannase, 10 parts of pectase；

Preparation method, comprises the following steps：

1) according to formula precise each component raw material, corn, wheat bran, oil cake of flax seed, clover are respectively dried to moisture and are contained It is 12% to measure, and is crushed, and crosses 80 mesh sieves, then uniformly mixes to obtain mixed powder A with trash fish powder, phagostimulant, wormwood seed powder；

2) chicken gizzard is cleaned, is cut into slices, 3h is soaked in the silk gum peptide solution that mass percent concentration is 1.4%, taken out, frozen It is dry, crush to obtain chicken liver meal；

3) the 50% of calcium berry extract quality is taken, is successively added thereto EM bacteria powders, feed complex enzyme, chicken liver meal, It is even to mix to obtain mixed powder B；

4) remaining calcium berry extract and salt are mixed, adds suitable quantity of water, be made the water that mass percent concentration is 25% Solution；

Embodiment 5

45 parts of corn, 35 parts of wheat bran, 25 parts of chicken gizzard, 25 parts of the flax dregs of rice, 15 parts of trash fish powder, 15 parts of clover, calcium berry extract 9 Part, 7 parts of phagostimulant, 3 parts of wormwood seed powder, 3 parts of feed complex enzyme, 0.8 part of EM bacteria powders, 0.8 part of salt, appropriate amount of water；

The feed complex enzyme is uniformly mixed by the raw material of following parts by weight：20 parts of yeast culture, wheat malt 30 parts of powder, 20 parts of acidic xylanase, 25 parts of acid protease, 15 parts of phytase, 20 parts of 1,4 beta-glucanase, cellulase 10 Part, 15 parts of amylase, 10 parts of 'beta '-mannase, 10 parts of pectase；

Preparation method, comprises the following steps：

1) according to formula precise each component raw material, corn, wheat bran, oil cake of flax seed, clover are respectively dried to moisture and are contained It is 8% to measure, and is crushed, and crosses 80 mesh sieves, then uniformly mixes to obtain mixed powder A with trash fish powder, phagostimulant, wormwood seed powder；

2) chicken gizzard is cleaned, is cut into slices, 3h is soaked in the silk gum peptide solution that mass percent concentration is 0.8%, taken out, frozen It is dry, crush to obtain chicken liver meal；

Embodiment 6

55 parts of corn, 45 parts of wheat bran, 35 parts of chicken gizzard, 35 parts of the flax dregs of rice, 25 parts of trash fish powder, 25 parts of clover, calcium berry extract 11 parts, 9 parts of phagostimulant, 5 parts of wormwood seed powder, 5 parts of feed complex enzyme, 1.2 parts of EM bacteria powders, 1.0 parts of salt, appropriate amount of water；

The feed complex enzyme is uniformly mixed by the raw material of following parts by weight：40 parts of yeast culture, wheat malt 20 parts of powder, 30 parts of acidic xylanase, 15 parts of acid protease, 25 parts of phytase, 10 parts of 1,4 beta-glucanase, cellulase 15 Part, 10 parts of amylase, 15 parts of 'beta '-mannase, 5 parts of pectase；

Preparation method, comprises the following steps：

1) according to formula precise each component raw material, corn, wheat bran, oil cake of flax seed, clover are respectively dried to moisture and are contained It is 12% to measure, and is crushed, and crosses 60 mesh sieves, then uniformly mixes to obtain mixed powder A with trash fish powder, phagostimulant, wormwood seed powder；

2) chicken gizzard is cleaned, is cut into slices, 1h is soaked in the silk gum peptide solution that mass percent concentration is 1.4%, taken out, frozen It is dry, crush to obtain chicken liver meal；

The culture efficiency experiment of the catfish feed of the present invention of embodiment 7

1. fingerling and its source：It is purchased from the native catfish of Ningxia aquaculture base；

2. the selection in fish pond is tested：In Ningxia Qingtongxia City production of catfish selected two mouthfuls of fish ponds respectively experimental group and Control group, fish pond area is 3 mu, and the cultivation depth of water is 2.0-2.5 meters.Test the cultivation abundant water in fish pond, the pure and fresh nothing of water quality Pollution.Fish pond sets independent culvert system, and water inlet gate-valve chamber sets three banister grooves, and water jacket installs coarse strainer, and inside groove installs taper Net, rhone installs anti-escape network.

3. test period：2015.3.20-2015.11.20

4. cultural method：Test group and control group use following cultural method：

(1) pond preparation

1. pond is leveled：Including the dry pool, desilting and solarization pond.

2. drug disinfection：7 days before fingerling stocking, change water with quick lime 100~130,000 gram/acre and splash sterilization of cleaning up the pond, it is desirable to Splash uniform, pond Bu Liu dead angles.

3. pond water filling：Water filling on the 5th before fingerling stocking, fill level 1.2 meters before fingerling stocking, after fingerling stocking Peak level is gradually filled to again.

(2) fingerling stocking：Experiment cultivation fingerling delivers the date on March 20th, 2015, during selection fingerling, it is desirable to fingerling body Mass color slide, without disability, fingerling embathes sterilization 3~5 minutes before entering pond with 5% saline solution.It is thrown to test group and control at random Group fish pond, input situation is shown in Table 1.

The soil Nian input situations of table 1

Fish pond	Kind	Specification (g/ tails)	Total mantissa	Average mu mantissa	Mu amount (Kg)	Total amount (Kg)
							Test group	Native catfish	50-100	9675	3225	241.9	725.6
Control group	Native catfish	50-100	9690	3230	242.3	726.8

(3) tame：Sat in the north facing the south in fishpond direction, with a distance from 2.5 meters from pond bank, away from 60 centimetres of bottom of pond, taken with asbestos shingle If 3~5 food platforms.The same day that fingerling enters pond starts domestication and feeds, and people sends sound before bait throwing in, makes fish Production conditions anti- Penetrate, when sound pauses, granular bait for fish is fed with wisp near bait table.2 times a day, 30~40 minutes every time, each bait throwing in Interval 10 seconds or so, so adheres to domestication 7~10 days, and fingerling ingests granular bait for fish quantity from less to more, and the intensity of feeding is by weak To strong transformation, until taming successfully.

(4) raise：Test group delivers catfish feed prepared by the embodiment of the present invention 2, and control group delivers commercially available same type, same Specification, the digestive utilization ratio catfish feed high with the date of manufacture.Feed 2 times daily, with 9:00―10:00、15:00―16:00 Respectively feed once.Bait should be thrown on food platform after fish are tamed.The feeding rate of fish is 2%~6%, and daily ration of feeding should be regarded and supported Grow concrete condition flexibly to grasp, be every time degree to be eaten up in 1 hour." four determine " principle should be also followed when feeding.

(5) daily management

1. regulating and controlling water quality：The preceding pond water level of fingerling stocking filled new water after fingerling stocking at 1.2 meters or so every 5~7 days Once.Add water 10~15 centimetres every time.The high temperature cultivation season water of 7-September adds to 2.0~2.5 meters, and former commonly using EM Dew improvement water quality, water transparency is 30~40 centimetres.

2. the pool is patrolled：It is daily patrol the pool and should follow early, middle and late patrol pool system.Check food situation, the variation of water of fish Deng pinpointing the problems and take measures in time.Particularly weather is sultry or has the situation of floating head omen to open aerator in time, keeps It is more than the mg/litre of water dissolved oxygen 5.

3. fish disease prevention and cure：Fish disease prevention and cure according to " put prevention first, prevent and treat combination " policy.Accomplish it is disease-free first prevent, cultivation Period is commonly used chlorine dioxide or lime disinfection water body.

5. pilot project and result：

Observation, detection catfish incidence, death condition are simultaneously noted down in the whole test period, statistical experiment group and control Group the fish pond incidence of disease and the death rate；Statistical experiment group and control group yield when going out the pool (mantissa, cabrage, gross weight, tail increase weight)； Result such as table 2：

Table 2：Native catfish feeding experiment result

Above result of the test shows：With same feeding and acclimation method, same daily management, same fish pond condition With feed catfish when same native catfish fingerling and injected volume, digestive utilization ratio catfish feed high prepared by the present invention is fitted Mouth property is strong, and adult fish specification gap is small, and neatly, and then catfish baits, competes the situation of looking for food slightly, and it is only 4% that the death rate is low, is compared 63.63% is reduced according to commercially available digestibility catfish feed high is organized；Disease resistance is strong, and the incidence of disease is only 3%, digestion high more commercially available than control group Rate catfish feed reduction by 66.67%；Digestibility is high, and tail increases weight by force, and digestibility catfish feed high more commercially available than control group is improved 55.74%；Feed coefficient is low, and feedstuff-meat ratio is low, digestibility catfish feed reduction by 35.29% high more commercially available than control group；Culture benefit Significantly, output increased 55.88% in the case of same injected volume, greatly reduces aquaculture cost, with preferable practicality and elder generation The property entered.

It should be noted that：Digestive utilization ratio catfish feed high prepared by embodiment of the present invention 3-6 equally has above-mentioned reality Effect is tested, it is between each embodiment and little with above-mentioned experiment effect otherness.

Bioactivity (stability) experiment of the catfish feed of the present invention of embodiment 8

The powdered protein enzyme of catfish feed prepared by the embodiment of the present invention 2 and commercially available identical date of manufacture is respectively at -12 DEG C and 40 DEG C under the conditions of store 12 months, use《GB/T23527-2009 National Standard of the People's Republic of China's protease preparations》 Middle detection method determines the enzyme activity of catfish feed and protease, calculates enzyme activity loss late, and enzyme activity loss late refers to actually detected The difference of enzyme activity and product mark enzyme activity account for the percentage of mark enzyme activity, as a result such as table 3

The Storage period enzyme activity loss late of table 3

Result above shows, is stored 12 months under the conditions of -12 DEG C and 40 DEG C, catfish feed of the present invention compared with commercially available, The loss of its proteinase activity reduces by 40% and 18.2% respectively, and with excellent temperature storage, enzyme activity is highly stable, while Also illustrate that the loss late of other bioactivators that catfish feed of the present invention contains is extremely low, with preferable freeze proof, heat resistance Can, product quality stabilization, long shelf-life.

It should be noted that：Catfish feed prepared by embodiment of the present invention 3-6 equally has above-mentioned experiment effect, each to implement It is between example and little with above-mentioned experiment effect otherness.

The catfish feed primary quality measure detection of the present invention of embodiment 9

Take the catfish feed of the preparation of the embodiment of the present invention 2 and the digestion profit high of commercially available same size, type and date of manufacture With rate catfish feed respectively as test group and control group, according to national aquatic granulated feed relevant criterion, detection moisture, moisture The leading indicators such as activity, pulverization rate, rate containing powder, hardness, gelatinization degree, testing result such as table 4：

Table 4：Catfish feed primary quality measure testing result

Project	Moisture (%)	Water activity	Pulverization rate (%)	Rate containing powder (%)	Hardness (g)	Gelatinization degree (%)
							Test group	5.7	0.283	1.2	0.3	8359.65	88
Control group	9.1	0.452	6.9	1.8	6337.82	48
							Difference	- 37.36%	- 37.38%	- 82.61%	- 66.67%	31.90%	83.3%

Result above shows：Compared with commercially available catfish feed, catfish Moisture in Feed content of the present invention and water activity are distinguished 37.36% and 37.38% is reduced, not easy infection miscellaneous bacteria, long shelf-life, good stability；The reduction of rate containing powder 66.67%, feed profit It is high with rate, lose small, be conducive to maintaining excellent water quality environment；Pulverization rate reduction by 82.61%, feed caking property is high, and particle is whole Together, glossiness is strong, is difficult, in transport and storage process friction efflorescence, to improve raw material availability, promotes the palatability of feed； Hardness improves 31.9%, and water resistance and durability degree are strong, are conducive to catfish to look for food, and extension is looked for food the time, improve efficiency of feed utilization And digestibility；Gelatinization degree is high, improves 83.3%, is easy to catfish to digest, absorb, and considerably improves the digestibility and utilization of catfish Rate.

Embodiment 10tlj2016 saccharomyces cerevisiae Cys tolerance is tested

By starting strain and each ring of tlj2016 slant strains, the 250mL equipped with 30mL Shake flask mediums is respectively connected to 150rpm in shaking flask, 30 DEG C are cultivated, when culture is to 12h, to the Guang ammonia of L- half that different final concentrations are added in shaking flask Acid, is further cultured for 10h, determines dry cell weight, as a result table 5,6；

Shake flask medium (g/L)：(NH₄)₂SO₄6th, glucose 20, K₂HPO₄·3H₂O 3、KH₂PO₄0.5th, dusty yeast 11, MnSO₄ 0.1、KCL 0.1、FeSO4 0.1、MgSO₄·7H₂O 0.1, pH6.0；

Table 5：Starting strain Cys tolerance

Table 6：Tlj2016L- cysteine tolerances

Cys concentration mmol/L	0	5	10	15	20	40
							Tlj2016 dry weights g/L	25.7	28.5	23.6	21.2	20.6	18.7
GSH concentration (mg/L)	73.2	98.3	113.5	121.7	127.5	135.8

Be can be seen that for starting strain from the result of table 5,6, Cys are added in culture medium, cell stops life It is long, and start self-dissolving, cause GSH growth rates to be reduced with the rising of Cys concentration；And the Guang ammonia of low concentration L- half Under acid, tlj2016 still is able to slow growth, and with the raising of Cys concentration, the dry cell weight of tlj2016 bacterial strains is slow Decline, and GSH concentration sustainable growths, this result is beneficial in GSH production processes by adding the Guangs of precusor amino acids-L- half Propylhomoserin proposes the production for promoting GSH.

Claims

1. a kind of digestive utilization ratio catfish feed high, is mainly prepared by the raw material of following parts by weight：Corn 40-60 parts, wheat bran 30-50 parts, chicken gizzard 20-40 parts, 20-40 parts of the flax dregs of rice, 10-30 parts of trash fish powder, clover 10-30 parts, 8-12 parts of calcium berry extract, Phagostimulant 6-10 parts, 2-6 parts of wormwood seed powder, feed complex enzyme 2-6 parts, 0.5-1.5 parts of EM bacteria powders, salt 0.6-1.2 parts, water In right amount；

The preparation method of the calcium berry extract, comprises the following steps：By calcium stem end, leaf of GAIGUO and inulin 5-9 in mass ratio:3- 6:1-3 uniformly mixes, and is placed in supersonic wave cleaning machine and cleans 3-5min in 200W, 30KHz, drains, and is crushed to particle diameter 0.3- 0.7mm, adds the water of 0.5-1.5 times of fragment quality, and regulation pH value is 5-7, adds the biology of mixture quality 0.1-0.3% Enzyme, 10-15min is digested in 50-60 DEG C；Enzymolysis liquid is concentrated under reduced pressure into solid content for 40-60%, be put into microwave dryer in 2000W, 130-150 DEG C carries out intermittent drying, is allowed to moisture and reaches 4-6%, is then crushed to particle diameter 0.4-0.6mm, adds The sodium acid carbonate of crushed material quality 0.2-0.4%, uniform mixing, adjustment mixture moisture is 15-18%, room temperature, sealing 2-4h is stood, in screw speed 105-115r/min, 140-160 DEG C of condition extrusion of temperature, then ultramicro grinding is to particle diameter 0.1-0.3mm obtains final product calcium berry extract；

The biology enzyme is cellulase, laccase, dextranase, seminase, pectase, tannase 2-4 in mass ratio:2-4: 1-3:1-3:0.5-1.5:0.4-1 uniformly mixes.

2. catfish feed as claimed in claim 1, it is characterised in that prepared by the main raw material by following parts by weight：Corn 45-55 parts, wheat bran 35-45 parts, chicken gizzard 25-35 parts, 25-35 parts of the flax dregs of rice, 15-25 parts of trash fish powder, clover 15-25 parts, calcium is really Extract 9-11 parts, phagostimulant 7-9 parts, 3-5 parts of wormwood seed powder, feed complex enzyme 3-5 parts, 0.8-1.2 parts of EM bacteria powders, salt 0.8-1.0 parts, appropriate amount of water.

3. catfish feed as claimed in claim 1, it is characterised in that prepared by the main raw material by following parts by weight：Corn 50 parts, 40 parts of wheat bran, 30 parts of chicken gizzard, 30 parts of the flax dregs of rice, 20 parts of trash fish powder, 20 parts of clover, 10 parts of calcium berry extract, phagostimulant 8 Part, 4 parts of wormwood seed powder, 4 parts of feed complex enzyme, 1 part of EM bacteria powders, 0.9 part of salt, appropriate amount of water.

4. the catfish feed as described in claim 1-3 is any, it is characterised in that the feed complex enzyme is by following parts by weight Raw material be uniformly mixed：It is yeast culture 20-40 parts, Wheat Sprout Powder 20-30 parts, acidic xylanase 20-30 parts, acid Protease 15-25 parts, phytase 15-25 parts, 1,4 beta-glucanase 10-20 parts, cellulase 10-15 parts, amylase 10-15 parts, 'beta '-mannase 10-15 parts, pectase 5-10 parts.

5. catfish feed as claimed in claim 4, it is characterised in that the preparation method of the yeast culture, including it is as follows Step：The saccharomyces cerevisiae tlj2016 slant strains after the activation of 1 ring are taken, the 500m L equipped with 100m L seed culture mediums are seeded to Cultivated in triangular flask, 25~30h is cultivated under the conditions of 28~30 DEG C, 150~200r/min of shaking speed, obtain first order seed Liquid；By primary seed solution by the inoculum concentration of 5~10% (v/v), the 500m L triangles equipped with 100m L fermentation mediums are seeded to Fermented and cultured is carried out in bottle, 25~30h of fermentation time, 28~30 DEG C of temperature, 150~200r/min of shaking speed obtains two grades Seed liquor；Secondary seed solution is sent out with the 5L fermentation tanks constant temperature that 5~10% (v/v) inoculum concentrations are linked into dress 3L fermentation mediums Ferment, 28~30 DEG C of temperature, 4~6L/min of ventilation, 150~200r/min of rotating speed, fermentation full adjustment zymotic fluid pH value are 6.0 ~6.5, when fermentation is to 25~30h, with every liter of addition of 25~30mmol of zymotic fluid to disposable addition L- in zymotic fluid Cysteine, continues fermentation 20-30h and obtains final zymotic fluid；Final zymotic fluid is concentrated under reduced pressure into solid content for 18- 26%, freeze-drying obtains final product yeast culture.

6. catfish feed as claimed in claim 5, it is characterised in that viable bacteria content is in the yeast culture：7×10¹²- 9×10¹²cfu/g。

7. catfish feed as claimed in claim 4, it is characterised in that the preparation method of the Wheat Sprout Powder, including following step Suddenly：Wheat is placed on temperature for 10-18 DEG C, pH value to soak 20-40min in the soak of 6-8, soak contains quality percentage Specific concentration is the sodium acid carbonate of 0.1-0.3% and the compound enzyme of 0.3-0.5%, is then warming up to 28- with the speed of 3-5 DEG C/min 32 DEG C, continue to soak 3-5h, 50-60 DEG C of immersion 5-8min is finally warming up to the speed of 5-7 DEG C/min, whole immersion process is every Every 15-20min ventilations once, ventilation pressure 0.13-0.15MPa, while in electric-field intensity 3-5kV/cm, burst length 100- 300 μ s, pulse frequency 60-80Hz condition carry out high-pressure pulse electric treatment, until wheat water content content is 40-45%；Immersion Wheat afterwards is drained, and dark germination is carried out in growing floor, and dark germination temperature is kept for 20-24 DEG C, and the dark germination time is 20-24h, warp Wheat drying to moisture after germination is 5-8%, and low-temperature grinding crosses 60-80 mesh sieves and obtains final product Wheat Sprout Powder；

8. the catfish feed as described in claim 1-3 is any, it is characterised in that the preparation method of the phagostimulant, including such as Lower step：1-3 in mass ratio:2-8 weighs fresh sheep blood and earthworm, mixes, rubs to obtain compound, adds 2-5 times of its quality Solion, mixes, and regulation pH value is 6-8, at room temperature in electric-field intensity 25-35kV/cm, burst length 300-500 μ s, arteries and veins Rushing under the conditions of frequency 200-300Hz carries out high-pressure pulse electric treatment 20-30min；It is subsequently adding mixture quality 0.04- 0.08% complex enzyme, 30-50min is digested in 50-60 DEG C, is then cooled to 35-45 DEG C of enzymolysis 40-60min, 90 DEG C of enzymes that go out 10min, is down to room temperature, and aseptic filtration obtains enzymolysis liquid, the ultrafiltration membrance filter that enzymolysis liquid passes through 1000Da and 5000Da, take two films it Between permeate, be concentrated under reduced pressure into solid content for 18-22% must digest concentrate；Add enzymolysis concentrate quality 2-4% Reduced sugar, successively in 112-118 DEG C react 30-50min and 135-145 DEG C reaction 45-65min after；Reaction solution is passed through The ultrafiltration membrance filter of 1000Da and 5000Da, takes the permeate between two films, is concentrated under reduced pressure into solid content for 26-30% is obtained Reaction concentrate；To the sericin peptide taken that its quality 0.5-1.5% is added in reaction concentrate, mix, freeze-drying obtains final product phagostimulant；

The solion is to contain sodium ion 17-22mg/L, zinc ion 15-20mg/L, potassium ion 12-14mg/L, calcium ion The aqueous solution of 9-11mg/L and magnesium ion 6-10mg/L；

The complex enzyme is earthworm serine protease, alkali protease, flavor protease, lipase 4-6 in mass ratio:2-4: 0.2-0.4:0.1-0.3 uniformly mixes.

9. catfish feed as claimed in claim 8, it is characterised in that the quality group of the reduced sugar turns into：Xylose:Ribose: Glucose:Fructose:Galactolipin=3-5:2-4:1-3:1-3:1-2.

10. the catfish feed as described in claim 1-3 is any, it is characterised in that the preparation method of the wormwood seed powder, including Following steps：By wormwood seed sabot, 8-10min is processed in electric-field intensity 1-3kV/cm high-pressure electrostatics first；Then it is in concentration Soaking at room temperature 2-5h in the salicylic acid solution of 6-8mg/L, while in electric-field intensity 2-6kV/cm, burst length 50-100 μ s, arteries and veins Rushing under the conditions of frequency 100-150Hz carries out high-pressure pulse electric treatment；Rinse, drain, 18-24h, Ran Houyi are stood in 3-5 DEG C It is secondary that 2-4d, -3--5 DEG C of freezing 1-3d, -15--18 DEG C of freezing 10-15h are refrigerated at 1-3 DEG C, it is immediately placed at outdoor natural light and shines 2- Crushed immediately after 4h, freezed, added the sodium bicarbonate of freeze-dried powder quality 3-8%, uniform mixing is crossed 80-100 mesh sieves and obtains final product wormwood artemisia Grass-seed powder.