CN106719442A - A kind of method that man-made drugs' control produces Artemia cysts - Google Patents
A kind of method that man-made drugs' control produces Artemia cysts Download PDFInfo
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- CN106719442A CN106719442A CN201611069293.4A CN201611069293A CN106719442A CN 106719442 A CN106719442 A CN 106719442A CN 201611069293 A CN201611069293 A CN 201611069293A CN 106719442 A CN106719442 A CN 106719442A
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- artemia
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Abstract
Have the present invention relates to the method that a kind of man-made drugs control produces Artemia cysts, including step：(1) configuration of Artemia cysts seawater；(2) hatching of artemia nauplii；(3) nursing of artemia nauplii and pseudimago early stage；(4) artemia imago is fed and is hatched, it is 20 ‰ 60 ‰ that seawater keeps salinity in whole breeding process, pH is 7.5 8.5, dissolved oxygen concentration is 4mg/L 6mg/L, water temperature is 24.5 26.5 DEG C, feed is grouper fermented feed during artemia imago is fed, while adding Fe EDTA in the seawater or adding trehalose, or adds Fe EDTA and trehalose simultaneously.The present invention is while salinity, pH, intensity of illumination, temperature, dissolved oxygen, feed nutrition factor is controlled, then Fe EDTA or trehalose are added in breeding seawater, solves the problems, such as that artemia resting egg yield under the conditions of propagating artificially is relatively low.
Description
Technical field
Artemia reproductive technology field of the present invention, a kind of method that especially man-made drugs' control produces Artemia cysts.
Background technology
Artemia nauplii is typically used as the life food of aquaculture, especially as ocean fish, morning in brine shrimp stage
The life food of phase, cut-off is current, aquaculture application mostly to freeze based on big artemia, Artemia cysts lie substantially in from
The situation so bred, manually fish for, the ovum that artemia is produced under natural conditions is divided into summer egg and resting egg, resting egg under natural conditions
The quantity of generation is very low, and resting egg exactly market sale, and the ovum of long-distance transport, market demand is very big, current user's purchase
It can only be single use to enter after Artemia cysts, it is impossible to enough to realize by hatching to nauplius, to pseudimago, to adult, then
To recycling for Artemia cysts, it is impossible to realize that Artemia cysts are manually produced, to the nursing of ocean fish, brine shrimp bring compared with
Production cost high, to solve the problems, such as that artemia produces resting egg wretched insufficiency, develops a kind of man-made drugs' control and produces artemia
The method of resting egg is always the direction that those skilled in the art make great efforts.
The content of the invention
It is an object of the invention to overcome the deficiencies in the prior art, there is provided a kind of man-made drugs' control produces Artemia cysts
Method.
The present invention solves its technical problem and takes following technical scheme to realize：
A kind of method that man-made drugs' control produces Artemia cysts, including step is as follows：
(1) configuration of Artemia cysts seawater：Running water adds sodium hypochlorite to be aerated 24 hours, and sea crystal configuration is added afterwards
It is 20 ‰ -60 ‰ artificial sea water into salinity, then proceedes to aeration 24 hours, the pH of control seawater is extra large between 7.5-8.5
Between 4mg/L---6mg/L, water temperature is between 24.5-26.5 DEG C for the concentration of water dissolves oxygen；
(2) hatching of artemia nauplii：The Artemia cysts that will be bought are put into the seawater for having configured, and are delivered density and are
The seawater that 1L is configured adds 1g artemia cysts, while adding 1g sodium acid carbonates, brooding time to keep illumination during being 24h, hatching
Intensity is put on to 28 DEG C water temperature between 1000-2000Lux, keeps constantly inflation preventing and treating artemia cysts to sink during hatching
Bottom, resting egg hatches into artemia nauplii completely after 24h；
(3) nursing of artemia nauplii and pseudimago early stage,
1. the human configuration sea conditions for cultivating be pH between 7.5-8.5, dissolved oxygen concentration is maintained at 4mg/L-6mg/L
Between, water temperature is maintained between 24.5 DEG C -26.5 DEG C；Intensity of illumination is maintained between 800-2000Lux；
2. cultivation density is 7-10/ml；
3. breeding bait and injected volume：
A. after artemia hatching within 24 hours without feeding, during the nauplius of-the five day second day, with Isochrysis galbana
Raise, each consumption is：During breeding water body 1L, the Isochrysis galbana 1ml after centrifugation, three times a day timing bait throwing in；
B. after nauplius during the pseudimago of-the ten day the 6th day, raised with epinephelus feed, each consumption is：Support
When growing water body 8L, the epinephelus feed that market is bought is pulverized last 0.5g, is fed at twice daily；
(4) artemia imago is fed and is hatched,
1. the seawater that will be configured during above-mentioned artemia nauplii and pseudimago keeps intensity of illumination in 800-2000Lux
Between；
2. breeding bait and injected volume：Raised with grouper fermented feed, each consumption of grouper fermented feed is：Cultivation
During water body 8L, each feeding volume is 12ml, twice a day timing bait throwing in；
3. in whole adult breeding process, Fe-EDTA is added in the seawater of above-mentioned cultivation, concentration reaches in making seawater
15mg-45mg/L, or trehalose is added, concentration reaches 5mg-15mg/L in making seawater, or adds Fe-EDTA simultaneously, makes seawater
Middle concentration reaches 15mg-45mg/L and adds trehalose, and concentration reaches 5mg-15mg/L in making seawater, and cultivation is opened after 12 days or so
Begin to lay eggs, the yield of Artemia cysts reaches more than 60%.
And, the B step nursing processes 3. walked in the step (3), and during the artemia imago of (4) step is fed often
It is secondary feed before, to carry out clear up residual bait and excrement.
And, the grouper fermented feed 2. walked in the step (3) is specifically included by mass fraction composition such as the following group
Part：0.5 part of 1 part of brown sugar, 20 parts of epinephelus feed, 500 parts of distilled water, 15 parts of sodium chloride and bacillus powder, said mixture
Aeration is after 4 hours, then through 30 DEG C of sealing and fermentings 24 hours, wherein epinephelus feed was bought by market.
And, the optimum salinity of the artificial seawater of all steps is 30 ‰.
And, optimal Fe-EDTA additions are that concentration reaches 30mg/L in making seawater in the 3. step of the step (4)；
Optimal trehalose addition is that concentration reaches 10mg/L in making seawater..
Advantages and positive effects of the present invention are：
The present invention is realized by controlling the same of the factors such as salinity, pH, intensity of illumination, temperature, dissolved oxygen, feed nutrition
When, then Fe-EDTA, trehalose are added, solve the problems, such as that artemia resting egg yield under the conditions of propagating artificially is relatively low.
Specific embodiment
The embodiment of the present invention is further described below, it is emphasized that, implementation below is illustrative, and
It is not limited, it is impossible in this embodiment as limitation of the invention.
A kind of method that man-made drugs' control produces Artemia cysts, including step is as follows：
(1) configuration of Artemia cysts seawater：Running water adds sodium hypochlorite to be aerated 24 hours, and sea crystal configuration is added afterwards
It is 20 ‰ -60 ‰ artificial sea water into salinity, then proceedes to aeration 24 hours, the pH of control seawater is extra large between 7.5-8.5
Between 4mg/L-6mg/L, water temperature is between 24.5-26.5 DEG C for the concentration of water dissolves oxygen；
(2) hatching of artemia nauplii：The Artemia cysts that will be bought are put into the seawater for having configured, and are delivered density and are
The seawater that 1L is configured adds 1g artemia cysts, while adding 1g sodium acid carbonates, brooding time to keep illumination during being 24h, hatching
Intensity is put on to 28 DEG C water temperature between 1000-2000Lux, keeps constantly inflation preventing and treating artemia cysts to sink during hatching
Bottom, resting egg hatches into artemia nauplii completely after 24h；
(3) nursing of artemia nauplii and pseudimago early stage,
1. the human configuration sea conditions for cultivating be pH between 7.5-8.5, dissolved oxygen concentration is maintained at 4mg/L-6mg/L
Between, water temperature is maintained between 24.5 DEG C -26.5 DEG C；Intensity of illumination is maintained between 800-2000Lux；
2. cultivation density is 7-10/ml；
3. breeding bait and injected volume：
A. after artemia hatching within 24 hours without feeding, during the nauplius of-the five day second day, with Isochrysis galbana
Raise, each consumption is：During breeding water body 1L, the Isochrysis galbana 1ml after centrifugation, three times a day timing bait throwing in；
B. after nauplius during the pseudimago of-the ten day the 6th day, raised with epinephelus feed, each consumption is：Support
When growing water body 8L, the epinephelus feed that market is bought is pulverized last 0.5g, is fed at twice daily, and feed every time
Before, clear up residual bait and excrement；
(4) artemia imago is fed and is hatched,
1. by the above-mentioned seawater holding intensity of illumination being configured between 800-2000Lux；
2. breeding bait and injected volume：Raised with grouper fermented feed, wherein, grouper fermented feed is specifically included：
1g brown sugar, 20g epinephelus feeds, distilled water 500ml, sodium chloride 15g and bacillus powder 0.5g, said mixture aeration 4 are small
When, 30 DEG C of sealing and fermentings 24 hours, each consumption of grouper fermented feed is：During breeding water body 8L, each feeding volume is 12ml,
Twice a day timing bait throwing in；
3. in whole breeding process, Fe-EDTA is added in the seawater of above-mentioned cultivation, concentration reaches in making seawater
15mg-45mg/L, or trehalose is added, concentration reaches 5mg-15mg/L in making seawater, or adds Fe-EDTA simultaneously, makes seawater
Middle concentration reaches 15mg-45mg/L and adds trehalose, and concentration reaches 5mg-15mg/L in making seawater, and cultivation is opened after 12 days or so
Begin to lay eggs, wherein, the yield of Artemia cysts reaches more than 60%.
In specific implementation of the invention, the optimum salinity of the artificial seawater of sea crystal configuration is in the step (1)
30‰。
In specific implementation of the invention, optimal Fe-EDTA additions are in making seawater in 3. the walking of the step (4)
Concentration reaches 30mg/L；Optimal trehalose addition is that concentration reaches 10mg/L in making seawater；
Embodiment one
The salinity of seawater is 7.5-8.5 for 30, pH；The concentration 4mg/L-6mg/L of dissolved oxygen；Water temperature is 24.5-26.5 DEG C；
Intensity of illumination is 800-2000Lux；Artemia culture initial density is 8/ml.Raised with Isochrysis galbana before artemia pseudimago
Support, consumption is：During breeding water body 8L, 8ml after centrifugation, three times a day timing bait throwing in；Raised with grouper in the artemia pseudimago phase
Material is raised, and consumption is：During breeding water body 8L, pulverize last 1g, twice a day timing bait throwing in.In the adult stage, with grouper
Fermented feed is raised, and each consumption is：During breeding water body 8L, 12ml, twice a day timing bait throwing in；
In adult stage water body also added with Fe-EDTA concentration be 30mg/L,
Any medicine group no added compared to the same period, egg laying rate of red swamp increased 53.8%
Embodiment two
The salinity of seawater is 7.5-8.5 for 30, pH；The concentration 4mg/L-6mg/L of dissolved oxygen；Water temperature is 24.5-26.5 DEG C；
Intensity of illumination is 800-2000Lux；Artemia culture initial density is 8/ml.Raised with Isochrysis galbana before artemia pseudimago
Support, consumption is：During breeding water body 8L, 8ml after centrifugation, three times a day timing bait throwing in；Raised with grouper in the artemia pseudimago phase
Material is raised, and consumption is：During breeding water body 8L, pulverize last 1g, twice a day timing bait throwing in.In the adult stage, with grouper
Fermented feed is raised, and each consumption is：During breeding water body 8L, 12ml, twice a day timing bait throwing in；
In adult stage water body also added with trehalose concentration be 10mg/L,
Any medicine group no added compared to the same period, egg laying rate of red swamp increased 49.3%
Embodiment three
The salinity of seawater is 7.5-8.5 for 30, pH；The concentration 4mg/L-6mg/L of dissolved oxygen；Water temperature is 24.5-26.5 DEG C；
Intensity of illumination is 800-2000Lux；Artemia culture initial density is 8/ml.Raised with Isochrysis galbana before artemia pseudimago
Support, consumption is：During breeding water body 8L, 8ml after centrifugation, three times a day timing bait throwing in；Raised with grouper in the artemia pseudimago phase
Material is raised, and consumption is：During breeding water body 8L, pulverize last 1g, twice a day timing bait throwing in.In the adult stage, with grouper
Fermented feed is raised, and each consumption is：During breeding water body 8L, 12ml, twice a day timing bait throwing in；
Also added with Fe-EDTA concentration for 15mg/L is simultaneously 5mg/L using trehalose concentration in adult stage water body.
Any medicine group no added compared to the same period, egg laying rate of red swamp increased 40.4%.
Claims (5)
1. a kind of method that man-made drugs' control produces Artemia cysts, it is characterised in that as follows including step：
(1) configuration of Artemia cysts seawater：Running water adds sodium hypochlorite to be aerated 24 hours, adds sea crystal to be configured to salt afterwards
The artificial sea water for 20 ‰ -60 ‰ is spent, aeration is then proceeded to 24 hours, controls the pH of seawater between 7.5-8.5, seawater is molten
The concentration of oxygen is solved between 4mg/L---6mg/L, water temperature is between 24.5-26.5 DEG C；
(2) hatching of artemia nauplii：The Artemia cysts that will be bought are put into the seawater for having configured, and deliver density for 1L matches somebody with somebody
The seawater put adds 1g Artemia cysts, while adding 1g sodium acid carbonates, brooding time to keep illumination during being 24h, hatching
Intensity is put on to 28 DEG C water temperature between 1000-2000Lux, keeps constantly inflation preventing and treating artemia cysts to sink during hatching
Bottom, resting egg hatches into artemia nauplii completely after 24h；
(3) nursing of artemia nauplii and pseudimago early stage,
1. the human configuration sea conditions for cultivating be pH between 7.5-8.5, dissolved oxygen concentration be maintained at 4mg/L-6mg/L it
Between, water temperature is maintained between 24.5 DEG C -26.5 DEG C；Intensity of illumination is maintained between 800-2000Lux；
2. cultivation density is 7-10/ml；
3. breeding bait and injected volume：
A. after artemia hatching within 24 hours without feeding, during the nauplius of-the five day second day, raised with Isochrysis galbana
Support, each consumption is：During breeding water body 1L, the Isochrysis galbana 1ml after centrifugation, three times a day timing bait throwing in；
B. after nauplius during the pseudimago of-the ten day the 6th day, raised with epinephelus feed, each consumption is：Cultivation water
During body 8L, epinephelus feed is pulverized last 0.5g, is fed at twice daily；
(4) artemia imago is fed and is hatched,
1. the seawater that will be configured during above-mentioned artemia nauplii and pseudimago keep intensity of illumination 800-2000Lux it
Between；
2. breeding bait and injected volume：Raised with grouper fermented feed, each consumption of grouper fermented feed is：Breeding water body
During 8L, each feeding volume is 12ml, twice a day timing bait throwing in；
3. in whole adult breeding process, Fe-EDTA is added in the seawater of above-mentioned cultivation, concentration reaches in making seawater
15mg-45mg/L, or trehalose is added, concentration reaches 5mg-15mg/L in making seawater, or adds Fe-EDTA simultaneously, makes seawater
Middle concentration reaches 15mg-45mg/L and adds trehalose, and concentration reaches 5mg-15mg/L in making seawater, and cultivation is opened after 12 days or so
Begin to lay eggs, the yield of Artemia cysts reaches more than 60%.
2. the method that man-made drugs' control according to claim 1 produces Artemia cysts, it is characterised in that：The step
(3) the B step nursing processes 3. walked in, and before being fed every time during the artemia imago of (4) step is fed, to carry out cleaning residual
Bait and excrement.
3. the method that man-made drugs' control according to claim 1 produces Artemia cysts, it is characterised in that：The step
(3) the grouper fermented feed 2. walked in specifically includes the following component constituted by mass fraction：1 part of brown sugar, epinephelus feed
0.5 part of 20 parts, 500 parts of distilled water, 15 parts of sodium chloride and bacillus powder are after said mixture is aerated 4 hours then close through 30 DEG C
Seal ferment 24 hours, wherein epinephelus feed is bought by market.
4. the method that man-made drugs' control according to claim 1 produces Artemia cysts, it is characterised in that：It is described all
The optimum salinity of the artificial seawater of step is 30 ‰.
5. the method that man-made drugs' control according to claim 1 produces Artemia cysts, it is characterised in that：The step
(4) optimal Fe-EDTA additions are that concentration reaches 30mg/L in making seawater in 3. step；Optimal trehalose addition is to make
Concentration reaches 10mg/L in seawater.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611069293.4A CN106719442A (en) | 2016-11-29 | 2016-11-29 | A kind of method that man-made drugs' control produces Artemia cysts |
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| CN201611069293.4A CN106719442A (en) | 2016-11-29 | 2016-11-29 | A kind of method that man-made drugs' control produces Artemia cysts |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109618990A (en) * | 2018-12-04 | 2019-04-16 | 天津海友佳音生物科技股份有限公司 | A kind of incubation fluid and its hatching method of high density shelling slag oxygenation |
| CN111289703A (en) * | 2020-04-14 | 2020-06-16 | 同济大学 | Method for testing ecological toxicity of disinfection by-product |
| CN114698600A (en) * | 2022-03-28 | 2022-07-05 | 浙江省淡水水产研究所 | Method for efficiently purifying artemia used in shrimp seedling raising period |
-
2016
- 2016-11-29 CN CN201611069293.4A patent/CN106719442A/en not_active Withdrawn
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109618990A (en) * | 2018-12-04 | 2019-04-16 | 天津海友佳音生物科技股份有限公司 | A kind of incubation fluid and its hatching method of high density shelling slag oxygenation |
| CN111289703A (en) * | 2020-04-14 | 2020-06-16 | 同济大学 | Method for testing ecological toxicity of disinfection by-product |
| CN114698600A (en) * | 2022-03-28 | 2022-07-05 | 浙江省淡水水产研究所 | Method for efficiently purifying artemia used in shrimp seedling raising period |
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Application publication date: 20170531 |