CN106719264B - Living body transportation method for propagation and releasing of octopus ocellatus seedlings - Google Patents
Living body transportation method for propagation and releasing of octopus ocellatus seedlings Download PDFInfo
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- CN106719264B CN106719264B CN201611188926.3A CN201611188926A CN106719264B CN 106719264 B CN106719264 B CN 106719264B CN 201611188926 A CN201611188926 A CN 201611188926A CN 106719264 B CN106719264 B CN 106719264B
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- 241000712522 Amphioctopus fangsiao Species 0.000 title claims abstract description 120
- 238000000034 method Methods 0.000 title claims abstract description 23
- 239000013535 sea water Substances 0.000 claims abstract description 125
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 76
- 235000009566 rice Nutrition 0.000 claims abstract description 76
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 73
- 241000047760 Octopus variabilis Species 0.000 claims abstract description 53
- 241000238413 Octopus Species 0.000 claims abstract description 19
- 239000002245 particle Substances 0.000 claims abstract description 19
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 90
- 239000001301 oxygen Substances 0.000 claims description 90
- 229910052760 oxygen Inorganic materials 0.000 claims description 90
- 241000209094 Oryza Species 0.000 claims description 75
- 239000006260 foam Substances 0.000 claims description 65
- 238000009413 insulation Methods 0.000 claims description 55
- 230000009471 action Effects 0.000 claims description 18
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 claims description 15
- 235000003642 hunger Nutrition 0.000 claims description 15
- 239000010903 husk Substances 0.000 claims description 14
- 238000001914 filtration Methods 0.000 claims description 11
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 claims description 10
- 231100000252 nontoxic Toxicity 0.000 claims description 10
- 230000003000 nontoxic effect Effects 0.000 claims description 10
- 238000004321 preservation Methods 0.000 claims description 10
- 238000002791 soaking Methods 0.000 claims description 10
- 238000011049 filling Methods 0.000 claims description 9
- 239000000463 material Substances 0.000 claims description 9
- 230000037351 starvation Effects 0.000 claims description 9
- 238000011177 media preparation Methods 0.000 claims description 7
- 239000004576 sand Substances 0.000 claims description 7
- 229920001971 elastomer Polymers 0.000 claims description 6
- 238000003860 storage Methods 0.000 claims description 6
- 239000012286 potassium permanganate Substances 0.000 claims description 5
- FFRBMBIXVSCUFS-UHFFFAOYSA-N 2,4-dinitro-1-naphthol Chemical compound C1=CC=C2C(O)=C([N+]([O-])=O)C=C([N+]([O-])=O)C2=C1 FFRBMBIXVSCUFS-UHFFFAOYSA-N 0.000 claims description 4
- 238000004140 cleaning Methods 0.000 claims description 4
- 229920006395 saturated elastomer Polymers 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims 2
- 239000002932 luster Substances 0.000 claims 1
- 230000004083 survival effect Effects 0.000 abstract description 22
- 230000007613 environmental effect Effects 0.000 abstract description 7
- 230000003444 anaesthetic effect Effects 0.000 abstract description 5
- 238000001816 cooling Methods 0.000 abstract description 5
- 235000013305 food Nutrition 0.000 abstract description 4
- 230000008859 change Effects 0.000 abstract description 3
- 230000000644 propagated effect Effects 0.000 abstract description 2
- 240000007594 Oryza sativa Species 0.000 abstract 1
- 239000011159 matrix material Substances 0.000 abstract 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 9
- 230000001706 oxygenating effect Effects 0.000 description 3
- 230000002035 prolonged effect Effects 0.000 description 3
- 238000003911 water pollution Methods 0.000 description 3
- 239000006004 Quartz sand Substances 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 230000035755 proliferation Effects 0.000 description 2
- 210000000707 wrist Anatomy 0.000 description 2
- 239000004475 Arginine Substances 0.000 description 1
- 241000252254 Catostomidae Species 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 210000000078 claw Anatomy 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 238000009364 mariculture Methods 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 239000005445 natural material Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 235000012239 silicon dioxide Nutrition 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/02—Receptacles specially adapted for transporting live fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
A living body transportation method for propagation and releasing of octopus ocellatus seedlings relates to the technical field of living body transportation of marine culture. The rice hulls are used as the matrix, are soft and cannot damage octopus ocellatus seedlings when being shaken, and can be suspended in seawater to form countless closed spaces, so that the probability of killing the octopus ocellatus seedlings by themselves is reduced; the water for feeding and transporting the octopus variabilis seedlings is used for removing suspended particles from seawater, and the seawater used for feeding and transporting the octopus variabilis seedlings is consistent with the seawater in the natural spawning ground sea area where the octopus variabilis seedlings are propagated and released, so that the octopus variabilis seedlings can adapt to the natural sea area environment as soon as possible, the influence of environmental change on the survival rate of the octopus variabilis seedlings is reduced, and the survival rate of the octopus variabilis is improved. The method has the advantages of no use of anesthetic, no food quality safety risk, no use of gradient cooling, no influence on survival rate of octopus fries, low cost, safety, environmental protection and high survival rate of the fries.
Description
Technical Field
The invention relates to the technical field of living body transportation of mariculture, in particular to a living body transportation method for propagation and releasing of octopus ocellatus seedlings.
Background
The octopus ocellatus is commonly called as the rice octopus ocellatus, the sitting octopus ocellatus, the short leg octopus ocellatus, the octopus ocellatus and the short claw octopus ocellatus, is mainly distributed in the Bohai sea, the yellow sea and the northeast sea, is delicious in meat quality, rich in arginine, short in life cycle, rapid in growth, high in nutritional value and economic value, and is deeply loved by people. Before and after the Ming festival every year, the octopus ocellatus gradually comes to the offshore place along with the wave, spawning and mating are carried out, and fishing flood is formed. However, in recent years, with the increasing market demand, the catching strength of octopus variabilis has been increased, so that natural resources are gradually exhausted. In order to protect natural resources and species genetic diversity of octopus variabilis, artificial breeding research of octopus variabilis is carried out in a few scientific research institutions, and in practical tests, the fact that the octopus variabilis are easy to adsorb on a container wall due to the fact that wrists and feet of the octopus variabilis are provided with two rows of suckers is found, and great inconvenience is brought to transportation; meanwhile, the mutual cannibalism phenomenon among the octopus ocellatus seedlings is serious, so that the survival rate of the octopus ocellatus seedlings is greatly reduced. At present, the living body transportation method for propagation and releasing of octopus variabilis fries is always in a blank state in the aquatic product industry.
Disclosure of Invention
The invention aims to provide a living body transportation method for propagation and releasing of octopus fries, which aims to achieve the purposes of no use of anesthetic, no use of gradient cooling, safety, environmental protection and high survival rate of the fries.
The invention provides a living body transportation method for propagation and releasing of octopus ocellatus seedlings, which is characterized by comprising the following steps:
step 1, medium preparation: rice hulls are used as a filling medium for transporting seawater;
step 2, preparing water: removing suspended particles in seawater for later use;
step 3, starvation treatment of octopus ocellatus seedlings: fasting treatment is carried out on the octopus ocellatus seedlings, so that excrement is discharged;
step 4, preparing a transport vessel and a vehicle:
step 4-1, selecting an oxygen bag and a foam insulation can made of non-toxic materials as transport vessels, putting seawater for removing suspended particles into the oxygen bag, adding rice hulls, putting octopus fries subjected to starvation treatment into the oxygen bag, and carrying out oxygen treatment according to the following steps: filling pure oxygen into the seawater =2: 1-4: 1 volume ratio, and then putting the oxygen bag into a foam insulation box;
step 4-2, selecting a van as a transport vehicle, stacking the foam heat preservation boxes in the step 4-1 in the van, and determining the number of the stacked foam heat preservation boxes according to the volume of the foam heat preservation boxes and the volume of the carriage of the van;
step 5, releasing the seedlings:
transporting the octopus variabilis seedlings to the sea area of a natural spawning ground of the octopus variabilis for releasing, opening a foam insulation box, shaking an oxygen bag, shaking the octopus variabilis seedlings adsorbed on the wall of the oxygen bag, enabling all the octopus variabilis seedlings to be dissociated in the seawater of the double-layer oxygen bag or adsorbed on the rice husk, opening the opening of the oxygen bag, and putting the octopus variabilis seedlings together with the rice husk and the seawater into the seawater;
the starvation treatment of the octopus ocellatus seedlings is to place the octopus ocellatus seedlings in a feeding pool for fasting treatment for 20-24 h, so that excrement is discharged; and moving the octopus ocellatus fries to a new feeding pond, putting seawater with the salinity of 29-31 per thousand, the water temperature of 20-23 ℃, the pH value of 7.9-8.1 and the water depth of 0.8-1.0 m into the feeding pond, and detecting the ammonia nitrogen content in the water body of the octopus ocellatus fries and the action speed of the octopus ocellatus fries after the octopus ocellatus fries grow in the new feeding pond for 1-2 h, so that the ammonia nitrogen content in the water body of the octopus ocellatus fries is reduced to less than 0.2mg/L and the action speed of the octopus ocellatus fries is reduced to less than 0.2 m/s.
The living body transportation method for propagation and releasing of the octopus variabilis fries provided by the invention is researched by combining the life habit of the octopus variabilis fries and the sea characteristics of propagation and releasing, and the advantages of the living body transportation method are mainly shown in the following aspects:
1. the rice hulls are used as media, are soft and cannot damage octopus ocellatus seedlings when being shaken, and can be suspended in seawater to form countless closed spaces, so that the probability of killing the octopus ocellatus seedlings by themselves is reduced; meanwhile, the rice hulls are rich in cellulose, lignin, silicon dioxide, fat, protein and the like, are natural, pollution-free, green and environment-friendly natural substances, can be naturally decomposed in seawater, are nontoxic and harmless to decomposers, cannot have adverse effect on the quality of the seawater, and are safe and environment-friendly;
2. the water for raising and transporting the octopus variabilis seedlings is used for removing suspended particles from seawater, and the seawater used for raising and transporting the octopus variabilis seedlings is consistent with the seawater in the natural spawning ground sea area where the octopus variabilis seedlings are propagated and released, so that the octopus variabilis seedlings can adapt to the natural sea area environment as soon as possible, the influence of environmental change on the survival rate of the octopus variabilis seedlings is reduced, and the survival rate of the octopus variabilis is improved;
3. starvation treatment is carried out on the octopus ocellatus seedlings, so that excrement is discharged completely, and the octopus ocellatus seedlings cannot be discharged in the transportation process after the excrement is discharged completely, so that the influence of the excrement on the quality of seawater in transportation and the influence on the survival rate of the octopus ocellatus seedlings can be avoided; the hunger-treated octopus ocellatus seedlings are slow in movement, so that the phenomenon of mutual cannibalism among the octopus ocellatus seedlings can be reduced; meanwhile, the octopus ocellatus fries are not treated by an anesthetic, so that the food quality safety risk is avoided; and gradient cooling is not used, and the survival rate of octopus ocellatus seedlings is not influenced;
4. selecting an oxygen bag and a foam insulation can made of non-toxic materials as transport vessels, and filling pure oxygen into the oxygen bag, wherein the pure oxygen in the oxygen bag can be supplied to octopus fries for breathing and survival; the foam insulation can keep the seawater in the oxygen bag at a proper temperature, provides an optimal living environment for octopus fries, and improves the survival rate of the octopus fries;
5. and during fry releasing, the octopus ocellatus fries are put into the seawater together with the rice husks and the seawater, so that the transportation environment and releasing environment of the octopus ocellatus fries are integrated, the influence of environmental change on the survival rate of the octopus ocellatus fries is reduced, and the survival rate of the fries is improved.
In conclusion, the method has the advantages of no use of anesthetic, no food quality safety risk, no use of gradient cooling, no influence on the survival rate of the octopus ocellatus fries, low cost, safety, environmental protection and high fry survival rate.
Detailed Description
The first embodiment is as follows:
step 1, medium preparation: rice husk is used as medium. Selecting rice hull which is newly produced in the current year and has the length of 7mm-10mm, the width of 3mm-5mm, the thickness of 25-30um and golden yellow or tawny color as a medium; cleaning the rice hulls for 3-5 times by using seawater, and soaking the rice hulls for 22-24 hours by using seawater with the salinity of 29-31 per mill to ensure that the rice hulls fully absorb water; then filtering the seawater in the rice hulls to dry, wherein the rice hulls are free of water dripping, and then using the rice hulls at 20X 10-6Soaking the rice hulls with potassium permanganate for 20min, and rinsing the rice hulls for 3-5 times by using seawater with salinity of 29-31 per mill for later use.
And 2, preparing by using water. Removing suspended particles in seawater by using a filtering sand jar, putting the seawater from which the suspended particles are removed into a clean water storage tank, then oxygenating the seawater to saturation by using 120-mesh nano-aerostones, adjusting the temperature of the seawater to be 20-23 ℃, adjusting the salinity of the seawater to be 29-31 per mill, and keeping the pH value to be 7.9-8.1 for later use.
And 3, starving octopus ocellatus seedlings. Placing the octopus ocellatus fries into a feeding pool to perform fasting treatment for 20-24 h, and discharging excrement; and moving the octopus ocellatus fries to a new feeding pond, putting seawater with the salinity of 29-31 per thousand, the water temperature of 20-23 ℃, the pH value of 7.9-8.1 and the water depth of 0.8-1.0 m into the feeding pond, and detecting the ammonia nitrogen content in the water body of the octopus ocellatus fries and the action speed of the octopus ocellatus fries after the octopus ocellatus fries grow in the new feeding pond for 1-2 h, so that the ammonia nitrogen content in the water body of the octopus ocellatus fries is reduced to less than 0.2mg/L and the action speed of the octopus ocellatus fries is reduced to less than 0.2 m/s. The ammonia nitrogen content in the water body is low, and the water pollution can be prevented. If the action speed of the octopus ocellatus fries is detected to be more than 0.2m/s, the time for fasting the octopus ocellatus fries can be properly prolonged, the physical strength of the octopus ocellatus fries is consumed, and the action speed of the octopus ocellatus fries is slowed down.
And 4, selecting a transport vessel and a vehicle. Transporting the octopus ocellatus fries in the morning when the temperature is relatively low at 5:00-7: 00. Step 4-1, selecting a double-layer oxygen bag and a foam insulation can made of non-toxic materials as a transport vessel, putting 7L-8L of seawater prepared by water in step 2 into a double-layer oxygen bag of 40 multiplied by 60cm, adding 0.8kg-1.2kg of rice husks prepared by the medium in step 1, putting 180-200 octopus seedlings with 8 mm-15 mm of hungry processed carcasses in step 3 into the double-layer oxygen bag, and carrying out oxygen treatment according to the following steps: the volume ratio of the seawater =2: 1-4: 1 is that pure oxygen is filled, so that the dissolved oxygen concentration of the seawater in the double-layer oxygen bag is increased to 10mg/L-13 mg/L; the bag mouth of the double-layer oxygen bag is tightened by a rubber band, the double-layer oxygen bag with the tightened bag mouth is placed into a foam insulation box with the size of 70cm multiplied by 30cm multiplied by 40cm, and each foam insulation box is placed into two double-layer oxygen bags. And 4-2, selecting a van as a transport vessel, stacking the foam insulation boxes in the step 4-1 in the van, and determining the number of the stacked foam insulation boxes according to the volume of the foam insulation boxes and the volume of the van carriage, wherein the total volume of the foam insulation boxes generally accounts for 80% -96% of the volume of the van carriage.
And 5, releasing the seedlings. And the fry releasing is to transport the fries of the octopus ocellatus to the sea area of the natural spawning site of the octopus ocellatus, and to select the sea area with the seawater transparency more than or equal to 5m, the sea current less than or equal to 0.5m/s, the water depth more than or equal to 10m, the salinity of 29 per thousand to 31 per thousand, the water temperature more than or equal to 20 ℃, and the pH value of 7.9 to 8.1. Transferring the foam insulation boxes to a ship from a van truck in sequence, driving the ship into an offshore sea area which is 3 km-5 km away and accords with the propagation and releasing of octopus variabilis seeds, opening the foam insulation boxes, shaking the oxygen bag, shaking the octopus variabilis seeds adsorbed on the wall of the oxygen bag to enable all the octopus variabilis seeds to be dissociated in the seawater of the double-layer oxygen bag or adsorbed on rice hulls, opening the bag opening of the double-layer oxygen bag, and putting the octopus variabilis seeds together with the rice hulls and the seawater into the seawater.
Example two:
step 1, medium preparation: rice husk is used as medium. Selecting rice hulls which are newly produced in the current year and have the length of 7mm-10mm, the width of 3mm-5mm, the thickness of 25-30um and golden yellow or tawny color as filling media for transporting seawater; cleaning the rice hulls for 3-5 times by using seawater without suspended particles, and soaking the rice hulls for 24 hours by using seawater with salinity of 29-31 per mill to ensure that the rice hulls fully absorb water; filtering the seawater in the rice hull with 20 mesh sieve, drying the rice hull without dripping water, and then using 20 × 10-6Soaking the rice hulls with potassium permanganate for 20min, and rinsing the rice hulls for 3-5 times by using seawater with salinity of 29-31 per mill for later use.
And 2, preparing by using water. Removing suspended particles in seawater by using a filtering sand cylinder, putting the seawater from which the suspended particles are removed into a clean water storage tank, then oxygenating by using 120-mesh nano-aerostones until the seawater is saturated, and adjusting the temperature of the seawater to be 20-23 ℃, wherein the temperature is preferably 21-22 ℃; adjusting the salinity of the seawater to 29-31 per mill, wherein 30 per mill is preferred; adjusting the pH value of the seawater to 7.9-8.1, preferably 8.0, for later use.
And 3, starving octopus ocellatus seedlings. Placing the octopus ocellatus fries into a feeding pond for 24h fasting treatment to drain excrement, moving the octopus ocellatus fries to a new feeding pond, placing seawater with salinity of 29-31 per thousand, water temperature of 20-23 ℃, pH of 7.9-8.0 and water depth of 0.8-1.0 m into the feeding pond, and detecting the ammonia nitrogen content in the water body of the octopus ocellatus fries and the action speed of the octopus ocellatus fries after the octopus ocellatus fries grow for 1h-2h in the new feeding pond, so that the ammonia nitrogen content in the water body of the octopus ocellatus fries is reduced to less than or equal to 0.2mg/L, and the action speed of the octopus ocellatus fries is reduced to less than or equal to 0.1 m/s. The ammonia nitrogen content in the water body is low, and the water pollution can be prevented. If the action speed of the octopus ocellatus fries is detected to be more than 0.1m/s, the time for fasting the octopus ocellatus fries can be properly prolonged, the physical strength of the octopus ocellatus fries is consumed, and the action speed of the octopus ocellatus fries is slowed down.
And 4, selecting a transport vessel and a vehicle. Transporting the octopus ocellatus fries in the morning when the temperature is relatively low at 5:00-7: 00. Step 4-1, selecting an oxygen bag and a foam insulation can made of non-toxic materials as transport vessels, putting 7L-8L of seawater prepared by using water in the step 2 and having water temperature, salinity and pH value adjusted into a double-layer oxygen bag of 40 multiplied by 60cm, adding 1.0kg of rice husks prepared by using the medium in the step 1, putting about 200 octopus seedlings with the carcass length of 8 mm-15 mm subjected to hunger treatment in the step 3 into the double-layer oxygen bag, and carrying out oxygen treatment according to the following steps: the volume ratio of the seawater =2: 1-3: 1 is that pure oxygen is filled, so that the dissolved oxygen concentration of the seawater in the double-layer oxygen bag is increased to 12 mg/L; the bag mouth of the double-layer oxygen bag is tightened by a rubber band, the double-layer oxygen bag with the tightened bag mouth is placed into a foam insulation box with the size of 70cm multiplied by 30cm multiplied by 40cm, and each foam insulation box is placed into two double-layer oxygen bags. And 4-2, selecting a van as a transport vessel, stacking the foam insulation boxes in the step 4-1 in the van, and determining the number of the stacked foam insulation boxes according to the volume of the foam insulation boxes and the volume of the van carriage, wherein the total volume of the foam insulation boxes generally accounts for 85% -95% of the volume of the van carriage. If too many foam insulation boxes are placed in the van compartment, the foam insulation boxes are not easy to stack and easily crush the foam insulation box at the bottommost layer; if the number of the foam insulation boxes placed in the van compartment is too small, the living body transportation cost of propagation and releasing of the octopus variabilis seedlings is increased.
And 5, releasing the seedlings. And the offspring seed releasing is to transport the offspring seeds of the octopus ocellatus to the sea area of a natural spawning site of the octopus ocellatus, and to select the sea area with the seawater transparency of more than or equal to 5m, the sea current of less than or equal to 0.3m/s, the water depth of more than or equal to 10m, the salinity of 29 per thousand to 31 per thousand, the water temperature of 20 to 23 ℃, and the pH value of 7.9 to 8.0 to release the offspring seeds, wherein the sea area with the seawater transparency of more than or equal to 5m, the sea current of less than or equal to 0.3m/s, the water depth of more than or equal to 10m, the salinity of 31 per thousand. Transferring the foam insulation boxes to a ship from a van truck in sequence, driving the ship into an offshore sea area which is 3 km-5 km away and accords with the propagation and releasing of the octopus variabilis seeds, opening the foam insulation boxes, shaking the oxygen bags, shaking the octopus variabilis seeds adsorbed on the walls of the double-layer oxygen bags to enable all the octopus variabilis seeds to be dissociated in the seawater of the double-layer oxygen bags or adsorbed on the rice hulls, opening the bag openings of the double-layer oxygen bags, and putting the octopus variabilis seeds together with the rice hulls and the seawater into the seawater.
The phi of the sand filtering cylinder is 3500 mm multiplied by 4300mm, and the flow rate is 11-140 m3And h, the bottom supporting layer phi is 1.5 mm-3 mm, the quartz sand, the upper dirt-intercepting layer phi is 0.5 mm-1 mm, and the quartz sand. The specification of the sand filtering cylinder can meet the requirement of removing suspended particles in seawater.
After the foam insulation box is placed in the oxygen bag, the box cover of the foam insulation box can be covered or uncovered, namely, the box cover is opened, wherein the box cover is preferably covered. The box cover of the foam heat preservation box is covered, so that the oxygen bag in the foam heat preservation box can keep proper temperature, the octopus fries can be supplied for survival, and the bearing capacity of the foam heat preservation box can be increased when the foam heat preservation box is stacked in a van carriage.
Example three:
transporting 12 ten thousand octopus seedlings with the average trunk length of about 1.2cm from a breeding base of the shore of the yellow sea of China to an adjacent 68km sea area for proliferation and releasing, wherein the survival rate of the octopus seedlings is as high as 98.5 percent, and the probability that the octopus seedlings adsorb the wall of the container is only 1.5 percent.
Step 1, medium preparation: rice husk is used as medium. Washing 600kg of rice hulls for 3-5 times by using seawater, and soaking the rice hulls for 24 hours by using seawater with salinity of 29-31 per mill to ensure that the rice hulls fully absorb water; filtering the seawater in the rice hull with 20 mesh sieve, drying the rice hull without dripping water, and then using 20 × 10-6Soaking the rice hulls with potassium permanganate for 20min, and rinsing the rice hulls for 3-5 times by using seawater with salinity of 29-31 per mill for later use.
And 2, preparing by using water. Removing suspended particles in seawater by using a filtering sand cylinder, putting the seawater from which the suspended particles are removed into a clean water storage tank, then oxygenating the seawater in the water storage tank until the seawater is saturated, and adjusting the temperature of the seawater to be 20-23 ℃, wherein the temperature is preferably 21-22 ℃; adjusting the salinity of the seawater to 29-31 per mill, wherein 30 per mill is preferred; adjusting the pH value of the seawater to 7.9-8.1, preferably 8.0, for later use.
And 3, starving octopus ocellatus seedlings. Selecting octopus ocellatus with average carcass length of 12mm, normal body color, intact wrist and strong mobility as the object for proliferation and releasing. Placing the octopus ocellatus fries into a feeding pond for 24h fasting treatment to drain excrement, moving the octopus ocellatus fries to a new feeding pond, placing seawater with salinity of 29-31 per thousand, water temperature of 20-23 ℃, pH of 7.9-8.1 and water depth of 0.8-1.0 m into the feeding pond, and detecting the ammonia nitrogen content in the water body of the octopus ocellatus fries and the action speed of the octopus ocellatus fries after the octopus ocellatus fries grow for 1h-2h in the new feeding pond, so that the ammonia nitrogen content in the water body of the octopus ocellatus fries is reduced to less than or equal to 0.2mg/L, and the action speed of the octopus ocellatus fries is reduced to less than or equal to 0.2 m/s. The ammonia nitrogen content in the water body is low, and the water pollution can be prevented. If the action speed of the octopus ocellatus fries is detected to be more than 0.2m/s, the time for fasting the octopus ocellatus fries can be properly prolonged, the physical strength of the octopus ocellatus fries is consumed, and the action speed of the octopus ocellatus fries is slowed down.
And 4, selecting a transport vessel and a vehicle. Transporting the octopus ocellatus fries in the morning when the temperature is lower at 5:00-7: 00. Step 4-1, selecting an oxygen bag and a foam insulation can made of non-toxic materials as a transport vessel, putting 8L of seawater prepared by using water in the step 2 and having water temperature, salinity and pH value adjusted into a 40 x 60cm double-layer oxygen bag, adding 1.0kg of rice hulls prepared by using the medium in the step 1, putting about 200 hungry-treated octopus seedlings with 12mm trunk length in the step 3 into the double-layer oxygen bag, squeezing out air in the bag, and carrying out oxygen treatment according to the following steps: filling pure oxygen into the seawater =2: 1-3: 1 volume ratio, and filling about 20L of pure oxygen to increase the dissolved oxygen concentration of the seawater in the double-layer oxygen bag to 12 mg/L; the bag mouth of the double-layer oxygen bag is tightened by a rubber band, the double-layer oxygen bag with the tightened bag mouth is placed into a foam insulation box with the size of 70cm multiplied by 30cm multiplied by 40cm, and each foam insulation box is placed into two double-layer oxygen bags. And 4-2, selecting a van as a transport vessel, stacking the foam insulation boxes in the step 4-1 in the van, wherein the size of the carriage of the van is 408 multiplied by 188 multiplied by 200cm, and 175 foam insulation boxes are placed in each van.
And 5, releasing the seedlings. And the offspring seed releasing is to transport the offspring seeds of the octopus ocellatus to the sea area of a natural spawning site of the octopus ocellatus, and to select the sea area releasing with the seawater transparency of more than or equal to 5m, the sea current of less than or equal to 0.3m/s, the water depth of more than or equal to 10m, the salinity of 29 per thousand to 31 per thousand, the water temperature of 20 to 23 ℃, and the pH value of 7.9 to 8.1, wherein the sea area releasing with the seawater transparency of more than or equal to 5m, the sea current of less than or equal to 0.3m/s, the water depth of more than or equal to 10m, the salinity of 31 per thousand, the water temperature of 21 to 22 ℃, and the. Transferring the foam insulation boxes to a ship from a van truck in sequence, driving the ship into an offshore sea area which is 3 km-5 km away and accords with the propagation and releasing of octopus variabilis seeds, opening the foam insulation boxes, shaking the oxygen bags, shaking the octopus variabilis seeds adsorbed on the bag walls of the double-layer oxygen bags to enable all the octopus variabilis seeds to be dissociated in the seawater of the double-layer oxygen bags or adsorbed on rice hulls, loosening rubber bands, opening bag openings of the double-layer oxygen bags, and putting the octopus variabilis seeds into the seawater together with the rice hulls and the seawater.
The transportation distance of the living body transportation method for propagation and releasing is 68km, the transportation time is 1.2h, the survival rate of the octopus ocellatus fries is as high as 98.5%, the vitality of the fries is high, the probability that the octopus ocellatus fries are adsorbed on the container wall is reduced to 1.5% from the former 63%, and the problems that the octopus ocellatus fries are adsorbed on the container wall, mutual cannibalism among the octopus ocellatus fries, the survival rate of the octopus ocellatus fries is low and the like during propagation and releasing of the octopus ocellatus frie.
A living body transportation method for propagation and releasing of octopus variabilis seeds relates to the technical field of living body transportation of marine culture, in particular to a living body transportation method for propagation and releasing of octopus variabilis seeds. The method is characterized by comprising the following steps: step 1, medium preparation: rice hulls are used as a medium; step 2, preparing water: removing suspended particles in seawater for later use; step 3, starvation treatment of octopus ocellatus seedlings: so that the excrement is discharged completely; step 4, preparing a transport vessel and a vehicle: selecting an oxygen bag and a foam insulation can made of non-toxic materials as a transport vessel; selecting a van as a transport vehicle; step 5, releasing the seedlings: transporting the octopus ocellatus fries to the natural spawning ground sea area of the octopus ocellatus for releasing, and putting the octopus ocellatus fries together with the rice husks and the seawater into the seawater. The method has the advantages of no use of anesthetic, no food quality safety risk, no use of gradient cooling, no influence on survival rate of octopus fries, low cost, safety, environmental protection and high survival rate of the fries.
Claims (6)
1. A living body transportation method for propagation and releasing of octopus ocellatus seedlings is characterized by comprising the following steps:
step 1, medium preparation: rice hulls are used as a filling medium for transporting seawater;
step 2, preparing water: removing suspended particles in seawater for later use;
step 3, starvation treatment of octopus ocellatus seedlings: fasting treatment is carried out on the octopus ocellatus seedlings, so that excrement is discharged;
step 4, preparing a transport vessel and a vehicle:
step 4-1, selecting an oxygen bag and a foam insulation can made of non-toxic materials as transport vessels, putting seawater for removing suspended particles into the oxygen bag, adding rice hulls, putting octopus fries subjected to starvation treatment into the oxygen bag, and carrying out oxygen treatment according to the following steps: filling pure oxygen into the seawater =2: 1-4: 1 volume ratio, and then putting the oxygen bag into a foam insulation box;
step 4-2, selecting a van as a transport vehicle, stacking the foam heat preservation boxes in the step 4-1 in the van, and determining the number of the stacked foam heat preservation boxes according to the volume of the foam heat preservation boxes and the volume of the carriage of the van;
step 5, releasing the seedlings:
transporting the octopus variabilis seedlings to the sea area of a natural spawning ground of the octopus variabilis for releasing, opening a foam insulation box, shaking an oxygen bag, shaking the octopus variabilis seedlings adsorbed on the wall of the oxygen bag, enabling all the octopus variabilis seedlings to be dissociated in the seawater of the double-layer oxygen bag or adsorbed on the rice husk, opening the opening of the oxygen bag, and putting the octopus variabilis seedlings together with the rice husk and the seawater into the seawater;
the starvation treatment of the octopus ocellatus seedlings is to place the octopus ocellatus seedlings in a feeding pool for fasting treatment for 20-24 h, so that excrement is discharged; and moving the octopus ocellatus fries to a new feeding pond, putting seawater with the salinity of 29-31 per thousand, the water temperature of 20-23 ℃, the pH value of 7.9-8.1 and the water depth of 0.8-1.0 m into the feeding pond, and detecting the ammonia nitrogen content in the water body of the octopus ocellatus fries and the action speed of the octopus ocellatus fries after the octopus ocellatus fries grow in the new feeding pond for 1-2 h, so that the ammonia nitrogen content in the water body of the octopus ocellatus fries is reduced to less than 0.2mg/L and the action speed of the octopus ocellatus fries is reduced to less than 0.2 m/s.
2. The live transportation method for propagation and releasing of Octopus ocellatus seedlings according to claim 1, wherein the preparation of the medium is carried out by using rice husks as medium, wherein rice husks which are newly produced in the current year, have a length of 7mm-10mm, a width of 3mm-5mm and a thickness of 25-30um and are golden yellow or tawny in color and luster are used as medium; cleaning the rice hulls for 3-5 times by using seawater, and soaking the rice hulls for 22-24 hours by using seawater with the salinity of 29-31 per mill to ensure that the rice hulls fully absorb water; then filtering the seawater in the rice hulls to dry, wherein the rice hulls are free of water dripping, and then using the rice hulls at 20X 10-6Soaking the rice hulls by potassium permanganate for 20min, and rinsing the rice hulls for 3-5 times by using seawater with salinity of 29-31 per mill.
3. The live transportation method for propagation and releasing of octopus variabilis seeds as claimed in claim 1, wherein said preparation for water use comprises removing suspended particles from seawater by using a sand filter vat, placing the seawater from which the suspended particles are removed into a clean water storage tank, adding oxygen until the seawater is saturated, adjusting the temperature of the seawater to 20-23 ℃, and adjusting the salinity of the seawater to 29-31 per mill, wherein the pH value is 7.9-8.1.
4. The live transportation method for propagation and releasing of Octopus ocellatus fry as claimed in claim 1, wherein the transportation vessel is made of oxygen bag and foam insulation can made of non-toxic material, 7L-8L of seawater is put into double-layered oxygen bag, 0.8kg-1.2kg of rice husk is added, and then starved Octopus ocellatus fry is put into oxygen bag according to oxygen: pure oxygen is filled in the seawater =2: 1-4: 1 volume ratio, so that the dissolved oxygen concentration of the seawater in the double-layer oxygen bag is increased to 10mg/L-13 mg/L; fastening the bag openings of the double-layer oxygen bags by using rubber bands, putting the double-layer oxygen bags with the fastened bag openings into foam insulation boxes, putting two double-layer oxygen bags into each foam insulation box, stacking the foam insulation boxes in the van, and determining the number of the stacked foam insulation boxes according to the volume of the foam insulation boxes and the volume of the van carriage.
5. The live transportation method for propagation and releasing of Octopus ocellatus fry as claimed in claim 1, wherein the fry releasing is to transport the Octopus ocellatus fry to the natural spawning site sea area of Octopus ocellatus, the transparency of sea water is greater than or equal to 5m, the sea current is less than or equal to 0.5m/s, the water depth is greater than or equal to 10m, the salinity is 29-31%, and the water temperature is greater than or equal to 20 ℃; opening the foam insulation box, shaking the oxygen bag, shaking the octopus variabilis fries adsorbed on the wall of the oxygen bag to enable the octopus variabilis fries to be completely dissociated in the seawater of the double-layer oxygen bag or adsorbed on the rice hulls, opening the opening of the oxygen bag, and putting the octopus variabilis fries together with the rice hulls and the seawater into the seawater.
6. The live transportation method for propagation and releasing of octopus variabilis seeds as claimed in claim 1, further comprising the steps of:
step 1, medium preparation: rice hulls are used as a medium;
selecting rice hulls which are newly produced in the current year and have the length of 7mm-10mm, the width of 3mm-5mm, the thickness of 25-30um and golden yellow or tawny color as filling media for transporting seawater;
cleaning the rice hulls for 3-5 times by using seawater, and soaking the rice hulls for 24 hours by using seawater with the salinity of 29-31 per mill to ensure that the rice hulls fully absorb water;
filtering the seawater in the rice hull with 20 mesh sieve, drying the rice hull without dripping water, and then using 20 × 10-6Soaking the rice hulls by potassium permanganate for 20min, and rinsing the rice hulls for 3-5 times by using seawater with salinity of 29-31 per mill for later use;
step 2, preparing water: removing suspended particles in seawater by using a filtering sand vat, putting the seawater from which the suspended particles are removed into a clean water storage tank, re-oxygenating until the seawater is saturated, adjusting the temperature of the seawater to be between 20 and 23 ℃, adjusting the salinity of the seawater to be between 29 and 31 per thousand, and adjusting the pH to be 7.9 to 8.0 for later use;
step 3, starvation treatment of octopus ocellatus seedlings: placing the octopus ocellatus fries into a feeding pond for 24-hour fasting treatment to drain excrement, moving the octopus ocellatus fries to a new feeding pond, placing seawater with the salinity of 29-31 thousandths, the water temperature of 20-23 ℃, the pH value of 7.9-8.0 and the water depth of 0.8-1.0 m into the feeding pond, and detecting the ammonia nitrogen content in the water body of the octopus ocellatus fries and the action speed of the octopus ocellatus fries after the octopus ocellatus fries grow for 1-2 hours in the new feeding pond, so that the ammonia nitrogen content in the water body of the octopus ocellatus fries is reduced to less than or equal to 0.2mg/L, and the action speed of the octopus ocellatus fries is reduced to less than or equal to 0.1 m/s;
and 4, selecting a transport vessel and a vehicle:
step 4-1, selecting an oxygen bag and a foam insulation can made of non-toxic materials as transport vessels, putting 7L-8L of seawater prepared by using water in the step 2 and having water temperature, salinity and pH value adjusted into a double-layer oxygen bag, adding 1.0kg of rice hulls prepared by using a medium in the step 1, putting 200 octopus seedlings with the carcass length of 8 mm-15 mm subjected to hunger treatment in the step 3, and carrying out oxygen: the volume ratio of the seawater =2: 1-3: 1 is that pure oxygen is filled, so that the dissolved oxygen concentration of the seawater in the double-layer oxygen bag is increased to 12 mg/L;
fastening the bag openings of the double-layer oxygen bags by using rubber bands, putting the double-layer oxygen bags with the fastened bag openings into foam insulation boxes, putting two double-layer oxygen bags into each foam insulation box, stacking the foam insulation boxes in the van, determining the number of the stacked foam insulation boxes according to the volume of the foam insulation boxes and the volume of the van carriage, wherein the total volume of the foam insulation boxes accounts for 85% -95% of the volume of the van carriage;
step 5, releasing the seedlings: transporting the octopus fries to the sea area of a natural spawning site of the octopus, and releasing the octopus fries in the sea area with the seawater transparency more than or equal to 5m, the ocean current less than or equal to 0.3m/s, the water depth more than or equal to 10m, the salinity of 29 per thousand to 31 per thousand, the water temperature of 20-23 ℃ and the pH value of 7.9-8.0; opening the foam insulation box, shaking the oxygen bag, shaking the octopus variabilis fries adsorbed on the wall of the oxygen bag to enable the octopus variabilis fries to be completely dissociated in the seawater of the double-layer oxygen bag or adsorbed on the rice hulls, opening the opening of the oxygen bag, and putting the octopus variabilis fries together with the rice hulls and the seawater into the seawater.
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