CN106719130A - It is a kind of to build the method that temperature and intensity of illumination influence model for ussuriensis prelarva viability - Google Patents
It is a kind of to build the method that temperature and intensity of illumination influence model for ussuriensis prelarva viability Download PDFInfo
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- 238000005286 illumination Methods 0.000 title claims abstract description 77
- 230000035899 viability Effects 0.000 title claims abstract description 50
- 238000000034 method Methods 0.000 title claims abstract description 22
- 238000002474 experimental method Methods 0.000 claims abstract description 35
- 241000251468 Actinopterygii Species 0.000 claims abstract description 18
- 238000012360 testing method Methods 0.000 claims abstract description 17
- 230000012447 hatching Effects 0.000 claims abstract description 8
- 230000001419 dependent effect Effects 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 238000005273 aeration Methods 0.000 claims description 2
- 230000000694 effects Effects 0.000 description 8
- 230000000384 rearing effect Effects 0.000 description 6
- 238000011160 research Methods 0.000 description 6
- 230000004083 survival effect Effects 0.000 description 6
- 238000013461 design Methods 0.000 description 3
- 230000000630 rising effect Effects 0.000 description 3
- 230000008859 change Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000013401 experimental design Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 241000881711 Acipenser sturio Species 0.000 description 1
- 241000208340 Araliaceae Species 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 241000252496 Siluriformes Species 0.000 description 1
- 241001663722 Tachysurus ussuriensis Species 0.000 description 1
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000013473 artificial intelligence Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 241001233037 catfish Species 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 230000026109 gonad development Effects 0.000 description 1
- 235000003642 hunger Nutrition 0.000 description 1
- 230000009916 joint effect Effects 0.000 description 1
- 230000001418 larval effect Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000037351 starvation Effects 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The method that temperature and intensity of illumination influence model for ussuriensis prelarva viability is built the present invention relates to a kind of, belongs to ussuriensis prelarva viability studying technological domain.The method comprises the following steps：The ussuriensis prelarva for just hatching 12 days is taken, the prelarva of normal condition is selected at room temperature, fish is used as experiment；Take the experiment that step one obtains and use fish, be divided into several experiment combinations of factors, the temperature and intensity of illumination of several experiment combinations of factors are different, and include that fish is used in a plurality of experiment in each experiment combinations of factors, tested afterwards；Off-test when prelarva is all dead, calculates not bait throwing in life force coefficientSAIValue；What is obtained is allSAIValue and its corresponding temperature and intensity of illumination, with temperature, intensity of illumination as independent variable, withSAIIt is dependent variable to be worth, and is fitted using least square method, and obtain temperature and intensity of illumination influences model for ussuriensis prelarva viability.
Description
Technical field
The invention belongs to ussuriensis prelarva viability studying technological domain, and in particular to one kind builds temperature and illumination
The method that intensity influences model for ussuriensis prelarva viability.
Background technology
Ussuriensis (Pseudobagrus ussuriensis) also known as crow Soviet Union in Catfish, alias cow's tail, Huang Angzi, return
Sturgeon, category SILURIFORMES, Chang sections, plan Chang category.It is distributed widely in the waters such as Heilungkiang, the Wusuli River, Nenjiang, Song Hua River, the Zhujiang River, flood
Damp lake, Taihu Lake are also distributed.Ussuriensis growth is fast, and feeding habits are wide, and meat flavour is delicious, is to cook and make chafing dish material without being pierced between flesh
Splendid top grade.Wild ussuriensis natural production is low, and variety source has been petered out, and artificial propagation is only relied in production,
The market price is higher.Less, research of the domestic scholars for the fingerling is reported in research both at home and abroad for ussuriensis at present
Artificial breeding technology exploitation are concentrated mainly on, rearing of fingerling, gonad development and stops up salinity scanning and consumption rate
Breath point etc., there is not yet the research that foreign scholar has correlation to this kind of fish is reported.So far it is not yet found that pass temperature and illumination are strong
Spend the research that model is influenceed for ussuriensis prelarva viability.
The content of the invention
The invention aims to solve the deficiencies in the prior art, and provide it is a kind of build temperature and intensity of illumination for
The method that ussuriensis prelarva viability influences model, the research method can provide reliable for the seed rearing of ussuriensis
Foundation.
The present invention is adopted the following technical scheme that：
It is a kind of to build the method that temperature and intensity of illumination influence model for ussuriensis prelarva viability including as follows
Step：
Step one：The ussuriensis prelarva for just hatching 1-2 days is taken, the prelarva of normal condition is selected at room temperature, made
For fish is used in experiment；
Step 2：Take the experiment that step one obtains and use fish, be divided into several experiment combinations of factors, and each experiment combinations of factors
It is interior to use fish including a plurality of experiment, tested afterwards；Off-test when prelarva is all dead, calculates not bait throwing in life force coefficient
SAI values, shown in the computing formula such as formula (I) of SAI values：
In formula (I), N is the prelarva number of starting；Number of days of the k for needed for prelarva all death；hiFor i-th day when prelarva it is tired
Meter death toll；
Step 3：All SAI values and its corresponding temperature and intensity of illumination that step 2 is obtained are taken, with temperature, light
According to intensity be independent variable, with SAI values as dependent variable, be fitted using least square method, obtain temperature and intensity of illumination for
Ussuriensis prelarva viability influences model.
Further, the temperature and intensity of illumination of each experiment combinations of factors are respectively uniformly distributed in 20- in step 2
32 DEG C, in 0-2000lux.
Further, each experiment combinations of factors is specifically configured to described in step 2：50 are developed normal and incubated
Change the prelarva of 1-2 days, be placed in and fill 1000mL running water and by the beaker that is aerated for 24 hours, except temperature and intensity of illumination not
Together, other conditions keep constant, observation prelarva situation, and record data daily.
Further, several experiment combinations of factors are 11 test combinations described in step 2：(20 26 32℃)*(0
1000 2000lux), central point combination (26 1000) is repeated 2 times.
Further, temperature described in step 3 and intensity of illumination influence model for ussuriensis prelarva viability
For：
SAI=-32.0718+3.1154T+6.8124 × 10-3I-2.625×10-5T×I-0.0628T2-3.2108×
10-6I2, model-fitting degree R2=0.9832, wherein T are temperature, and I is intensity of illumination.
The present invention also provides one kind influences model by structure temperature and intensity of illumination for ussuriensis prelarva viability
Method to build the temperature that obtains and intensity of illumination young in ussuriensis for ussuriensis prelarva viability influence model
Application in fish culture, specially：Temperature and intensity of illumination are asked for ussuriensis prelarva viability influence model
Solution, when calculating SAI values maximum, corresponding temperature and intensity of illumination, the temperature and intensity of illumination are ussuriensis son
The optimal cultivating condition of fish；If SAI values are maximum, corresponding temperature and intensity of illumination have multigroup, then according to actual conditions
The minimum group of alternative costs as ussuriensis prelarva optimal cultivating condition.
Compared with prior art, beneficial effects of the present invention：
The present invention is studied ussuriensis prelarva viability, structure at temperature and intensity of illumination collective effect
Built temperature and intensity of illumination influences model for ussuriensis prelarva viability, to improve ussuriensis seed rearing matter
Amount provides reliable basis：After treating that ussuriensis prelarva hatches, it is in temperature 25 DEG C of (± 1), intensities of illumination by it
960lux is cultivated under (± 50), can greatly improve the survival rate of prelarva；The model is in different temperatures and intensity of illumination
Cultivation ussuriensis prelarva provides reliable basis in area, can improve seed rearing quality, reduces cultivation loss；Meanwhile, this
The model prediction accuracy for inventing structure is higher, for the structure of related ussuriensis prelarva viability influence model provides ginseng
Examine.
Figure of description
Fig. 1 is the response surface design figure of temperature and intensity of illumination to SAI；
Fig. 2 is the contour map of temperature and intensity of illumination to SAI.
Specific embodiment
With reference to embodiment, the present invention is described in further detail.
It will be understood to those of skill in the art that the following example is merely to illustrate the present invention, and should not be regarded as limiting this hair
Bright scope.
It is a kind of to build the method that temperature and intensity of illumination influence model for ussuriensis prelarva viability including as follows
Step：
Step one：The ussuriensis prelarva for just hatching 1-2 days is taken, the prelarva of normal condition is selected at room temperature, made
For fish is used in experiment；
Step 2：Take the experiment that step one obtains and use fish, be divided into several experiment combinations of factors, and each experiment combinations of factors
It is interior to use fish including a plurality of experiment, tested afterwards；Off-test when prelarva is all dead, calculates not bait throwing in life force coefficient
SAI values, shown in the computing formula such as formula (I) of SAI values：
In formula (I), N is the prelarva number of starting；Number of days of the k for needed for prelarva all death；hiFor i-th day when prelarva it is tired
Meter death toll；
Step 3：All SAI values and its corresponding temperature and intensity of illumination that step 2 is obtained are taken, with temperature, light
According to intensity be independent variable, with SAI values as dependent variable, be fitted using least square method, obtain temperature and intensity of illumination for
Ussuriensis prelarva viability influences model.Wherein, the temperature and intensity of illumination of each experiment combinations of factors are divided in step 2
Be not evenly distributed on 20-32 DEG C, in 0-2000lux.Each experiment combinations of factors is specifically configured to described in step 2：By 50
Development is normal and prelarva of hatching 1-2 days, is placed in and fills 1000mL running water and by the beaker that is aerated for 24 hours, except temperature
Different with intensity of illumination, other conditions keep constant, observation prelarva situation, and record data daily.Number described in step 2
Individual experiment combinations of factors is 11 test combinations：(20 26 32 DEG C) * (0 1000 2000lux), central point combination (26
1000) it is repeated 2 times, temperature described in step 3 and intensity of illumination are for ussuriensis prelarva viability influence model：
SAI=-32.0718+3.1154T+6.8124 × 10-3I-2.625×10-5T×I-0.0628T2-3.2108×
10-6I2, model-fitting degree R2=0.9832, wherein T are temperature, and I is intensity of illumination.
The present invention also enters on the temperature and intensity of illumination that obtain for ussuriensis prelarva viability influence model simultaneously
Row is solved, and when calculating SAI values maximum, corresponding temperature and intensity of illumination, the temperature and intensity of illumination are in Usu and intend
The optimal cultivating condition of Chang prelarvas；If SAI values are maximum, corresponding temperature and intensity of illumination have multigroup, then according to reality
The minimum group of situation alternative costs as ussuriensis prelarva optimal cultivating condition.
Embodiment 1
The ussuriensis prelarva of 1-2 days is just hatched, the parent population warp cultivated by Huaian Aquatic Products Science Inst. is remarkable
Work fertilization, hatching gained.The prelarva of normal condition is selected at room temperature, and fish is used as experiment.
According to the design experiment scheme of table 1,2000mL beakers are loaded into 1000mL running water and by exposure in 24 hours before experiment
Gas (pH 7, dissolved oxygen>4.5mg.L-1, ammonia nitrogen<0.06mg.L-1), after be positioned over artificial intelligence climate box (model RTOP-150Y)
It is interior, corresponding temperature and intensity of illumination are set in advance.Experiment prelarva is placed into after coolant-temperature gage reaches relative set.
Designed using Central Composite, study the connection of temperature and intensity of illumination double factor for ussuriensis prelarva viability
Group photo ring or effect, totally 11 combination (202632 DEG C) * (010002000lux), central point combination (26,1000) be repeated 2 times with
Estimate pure test error, specific combination and result of the test are shown in Table 1.Using this experimental design, each test factor takes 3 respectively
Level, axle points are 4, and factor points 4, center points are set to 3, and asterisk arm lengths are | 1 |.To ensure reliable results, each
Axle point and factor point combination 1 repetition of each setting.The viability of prelarva is with not bait throwing in viability coefficient S AI (survival
Activity index) it is measurement index.Every group of experiment combinations of factors is put into 50 normotrophic prelarvas, is placed on above-mentioned
Fill 1000mL running water and by 24 hours aeration beaker in, except temperature is different with intensity of illumination in process of the test, its
Its condition keeps constant.Observation prelarva situation, and record data daily.Off-test is calculated as follows SAI values：
In formula, N is the prelarva number of starting；Number of days of the k for needed for prelarva all death；hiFor i-th day when, prelarva it is accumulative
Death toll.Statistical analysis is carried out to it again after the SAI values for obtaining each test factor combination.
Test error assumes to obey the normal distribution that average is 0, and every effect uses Least Square Method in model.Examination
Test data to be processed using SAS (V9.13) statistical software, every Effect Estimation in model conspicuousness and appropriateness, model
Conspicuousness is tested using method of analysis of variance.The degree of fitting of model is with coefficient of determination R2Weigh.It is two examinations of clear observation
The joint effect of factor pair SAI is tested, present invention also provides response surface design figure and contour map.Significance is set in experiment
It is 5%.
Two factor level combinations and corresponding result of the test in the experimental design of table 1
As seen from Figure 1：
1st, influence of the different temperatures for ussuriensis prelarva viability
In 20 DEG C to 25 DEG C intervals, with the rising of temperature, life force coefficient increases；25 DEG C afterwards, with temperature
Raise, the reduction of life force coefficient.Effect of temperature influences extremely significantly (P for ussuriensis prelarva viability<0.01),
The quadratic effect of temperature is for ussuriensis prelarva viability index impacts extremely significantly (P<0.01).
2nd, influence of the different illumination intensity for ussuriensis prelarva viability
Intensity of illumination in the range of 0lux--1000lux, with the rising of intensity of illumination, on the life force coefficient of prelarva
Rise；In the range of 1000lux--2000lux, with the rising of intensity of illumination, the life force coefficient of prelarva declines.Intensity of illumination
Effect (P not notable for the viability index impacts of ussuriensis prelarva>0.05), the quadratic effect pair of intensity of illumination
With the viability index impacts extremely significantly (P of ussuriensis prelarva<0.01).
3rd, the influence of different illumination intensity, temperature for ussuriensis prelarva viability
Mutual work between temperature and intensity of illumination is to the viability index impacts of ussuriensis prelarva not significantly (P>0.05).
Show that temperature and intensity of illumination influence model for ussuriensis prelarva viability：
SAI=-32.0718+3.1154T+6.8124 × 10-3I-2.625×10-5T×I-0.0628T2-3.2108×
10-6I2, model-fitting degree R2=0.9832, wherein T are temperature, and I is intensity of illumination.
Typically, after fish prelarva membrane, the stress such as starvation, temperature, illumination, pH, therefore resistance to hungry ability and work can be subject to
The strong prelarva of power, can preferably adapt to environment in growing, and increase the possibility of survival.
Not bait throwing in viability coefficient S AI values are often used as the finger of differentiation larval activity and quality-critical during fry rearing
One of mark, is applied in more research.In the range of suitable ecological factor, the SAI values of prelarva are high, otherwise SAI values
It is relatively low.As seen from Figure 1, the SAI values of prelarva are reduced afterwards as the change of temperature is first raised, and at 25 DEG C, the SAI indexes of prelarva are most
Height, illustrates at a temperature of this, the viability of prelarva is best, so after treating that ussuriensis prelarva hatches, can be placed
In 25 DEG C of water of (± 1) of water temperature, the survival rate of prelarva can be greatly improved.
As seen from Figure 1, intensity of illumination be 960lux when, prelarva SAI indexes now are higher, illustrate this intensity of illumination it
Under, the viability of prelarva is best.The height of prelarva SAI values, is the important indicator of nursery result after determining.Treat ussuriensis
After prelarva hatches, intensity of illumination is placed on for 960lux (± 50) place, the survival rate of prelarva can be made higher.
SAI values are bigger, and the vigor of prelarva is better, and viability when using it for seed rearing is higher.When using temperature and
Intensity of illumination double factor for prelarva synergy when, as a result show：When temperature is 25 DEG C, and intensity of illumination is 960lux,
SAI index highests, now the viability highest of fish.After prelarva hatching, put and cultivated with this understanding, the survival rate of prelarva is
Highest.
It should be understood by those skilled in the art that, the present invention is not limited to the above embodiments, above-described embodiment and explanation
Merely illustrating the principles of the invention described in book, without departing from the spirit and scope of the present invention, the present invention also has
Various changes and modifications, these changes and improvements all fall within the protetion scope of the claimed invention.The claimed scope of the invention
By appending claims and its equivalent thereof.
Claims (6)
1. a kind of to build the method that temperature and intensity of illumination influence model for ussuriensis prelarva viability, its feature exists
In comprising the following steps：
Step one：The ussuriensis prelarva for just hatching 1-2 days is taken, the prelarva of normal condition is selected at room temperature, as examination
Test and use fish；
Step 2：Take the experiment that step one obtains and use fish, be divided into bag in several experiment combinations of factors, and each experiment combinations of factors
Include a plurality of experiment and use fish, tested afterwards；Off-test when prelarva is all dead, calculates not bait throwing in viability coefficient S AI values,
Shown in the computing formula of SAI values such as formula (I)：
In formula (I), N is the prelarva number of starting；Number of days of the k for needed for prelarva all death；hiFor i-th day when prelarva it is accumulative dead
Die number；
Step 3：All SAI values and its corresponding temperature and intensity of illumination that step 2 is obtained are taken, it is strong with temperature, illumination
It is independent variable to spend, and with SAI values as dependent variable, is fitted using least square method, obtains temperature and intensity of illumination for Usu
In intend Chang prelarvas viabilitys influence model.
2. temperature and the intensity of illumination of building according to claim 1 is for ussuriensis prelarva viability influence model
Method, it is characterised in that the temperature and intensity of illumination of each experiment combinations of factors are respectively uniformly distributed in 20-32 in step 2
DEG C, in 0-2000lux.
3. temperature and the intensity of illumination of building according to claim 1 is for ussuriensis prelarva viability influence model
Method, it is characterised in that each experiment combinations of factors is specifically configured to described in step 2：Normal and hatching 1- is developed by 50
The prelarva of 2 days, be placed in fill 1000mL running water and by 24 hours aeration beaker in, except temperature is different with intensity of illumination,
Other conditions keep constant, observation prelarva situation, and record data daily.
4. temperature and the intensity of illumination of building according to claim 1 is for ussuriensis prelarva viability influence model
Method, it is characterised in that several experiment combinations of factors are 11 test combinations described in step 2：(202632℃)*
(010002000lux), central point combination (26,1000) is repeated 2 times.
5. temperature and the intensity of illumination of building according to claim 4 is for ussuriensis prelarva viability influence model
Method, it is characterised in that temperature described in step 3 and intensity of illumination are for ussuriensis prelarva viability influence model：
SAI=-32.0718+3.1154T+6.8124 × 10-3I-2.625×10-5T×I-0.0628T2-3.2108×10-6I2,
Model-fitting degree R2=0.9832, wherein T are temperature, and I is intensity of illumination.
6. the structure temperature and intensity of illumination as described in any one of claim 1 to 5 are for ussuriensis prelarva viability shadow
The method for ringing model builds the temperature that obtains and intensity of illumination and influences model in Usu for ussuriensis prelarva viability
Intend the application in the cultivation of Chang prelarvas, it is characterised in that：Temperature and intensity of illumination are influenceed for ussuriensis prelarva viability
Model is solved, when calculating SAI values maximum, corresponding temperature and intensity of illumination, and the temperature and intensity of illumination are crow
Intend the optimal cultivating condition of Chang prelarvas in Soviet Union；If SAI values are maximum, corresponding temperature and intensity of illumination have multigroup, then root
According to the minimum group of actual conditions alternative costs as ussuriensis prelarva optimal cultivating condition.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000201568A (en) * | 1999-01-18 | 2000-07-25 | Hitachi Ltd | Automatic feeding system for aquatic organism |
| CN101720682A (en) * | 2008-10-24 | 2010-06-09 | 中国科学院水生生物研究所 | Lake mandarin fish scale culturing method |
| CN104905030A (en) * | 2015-05-20 | 2015-09-16 | 东北农业大学 | Special compound feed for pseudobagrus ussuriensis fries and advanced fries and a preparation method thereof |
| CN105052786A (en) * | 2015-07-30 | 2015-11-18 | 浙江海洋学院 | Optimum selection method of wild parent of autumn shrimp of Marsupenaeus japonicus and fundamental group morphological model obtaining method |
| CN105210947A (en) * | 2015-10-16 | 2016-01-06 | 中国农业大学 | A kind of regulate and control method improving and promote fancy fishes sign quality |
-
2016
- 2016-11-25 CN CN201611062475.9A patent/CN106719130B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000201568A (en) * | 1999-01-18 | 2000-07-25 | Hitachi Ltd | Automatic feeding system for aquatic organism |
| CN101720682A (en) * | 2008-10-24 | 2010-06-09 | 中国科学院水生生物研究所 | Lake mandarin fish scale culturing method |
| CN104905030A (en) * | 2015-05-20 | 2015-09-16 | 东北农业大学 | Special compound feed for pseudobagrus ussuriensis fries and advanced fries and a preparation method thereof |
| CN105052786A (en) * | 2015-07-30 | 2015-11-18 | 浙江海洋学院 | Optimum selection method of wild parent of autumn shrimp of Marsupenaeus japonicus and fundamental group morphological model obtaining method |
| CN105210947A (en) * | 2015-10-16 | 2016-01-06 | 中国农业大学 | A kind of regulate and control method improving and promote fancy fishes sign quality |
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