CN106718829A - A kind of Sweet Corn Germplasm preservation and the method for reproduction and renewal - Google Patents
A kind of Sweet Corn Germplasm preservation and the method for reproduction and renewal Download PDFInfo
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- CN106718829A CN106718829A CN201611069963.2A CN201611069963A CN106718829A CN 106718829 A CN106718829 A CN 106718829A CN 201611069963 A CN201611069963 A CN 201611069963A CN 106718829 A CN106718829 A CN 106718829A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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Abstract
Preserved the present invention relates to a kind of Sweet Corn Germplasm and reproduction and renewal method, the Sweet Corn Germplasm self-mating system kind that will be preserved in field, and by female fringe bagging；Genetic background identical conventional corn self-mating system pollen is taken, by pollen pollination in the corn female fringe of bagging；Autumn harvests pollination fruit ear, obtains corn and conventional corn F1 generation cenospecies；F1 generation cenospecies is stored in warehouse；After 35 years, F1 generation cenospecies is taken out, planted in field, and bagging selfing；Selfing fruit ear is harvested, is dried, select shrinkage seed on fruit ear, obtain final product Sweet Corn Germplasm seed.The Sweet Corn Germplasm seed and original Sweet Corn Germplasm of acquisition are planted in field jointly, and it is consistent that breeding time compares both External Characters.Using the inventive method in the warehouse of general nature condition, Sweet Corn Germplasm can be stored 35 years, and the Sweet Corn Germplasm for obtaining can keep its primary characteristic not change, can cost-effective and holding germination percentage higher.
Description
Technical field
The present invention relates to a kind of preservation of Sweet Corn Germplasm and the method for reproduction and renewal, belong to agriculture Germ-plasma resources protection neck
Domain.
Background technology
Corn is sweet and delicious, is liked by people, more there is a kind of " fruit type corn ", can be eaten something rare as fruit.It is sweet
Total amino acid content is higher than conventional corn and waxy corn by 23.2% and 12.7% respectively in corn, in amino acid composition, to rely ammonia
The content of sour, tryptophan is higher, and higher than conventional corn more than 2 times, 8 kinds of essential amino acid total amounts then compare conventional corn respectively
23.5% and 6.6% are higher by with waxy corn, and essential amino acid proportion of composing is more balanced；Protein content reaches 13%
More than, based on water-solubility protein, crude fat content is higher by l times or so up to 9.9% than conventional corn；Glucose, sucrose, fruit
Sugared content is 2-8 times of conventional corn, and VB1, VB2~VB5, VB6, VC, VE, carrotene and 18 kinds of amino acid are rich in seed
Deng nutrient.
Different according to genetic characteristicses, corn is divided into common corn, super-sweet corn and strengthens corn.Common corn
It is by single recessive sweet -1 gene (su1) control.Super-sweet corn is by the control of -2 (sh2) genes of single recessive depression or -2 (bt2) of fragility
Gene is controlled.It is the control of double recessive (su1se) gene to strengthen corn.
Corn germ plasm resource is the material base of seed selection improved seeds, strengthens grinding effective utilization of corn germ plasm resource
Study carefully, to widening corn germplasm hereditary basis, improve breeding of sweet level and play an important roll.Extension seed storage to greatest extent
Hide the effective way that the life-span is protection germ plasm resource.
And Sweet Corn Germplasm due to after maturation dry matter content it is few, so storage it is relatively difficult.In natural draft drying
Under the conditions of warehouse, 1-2 can be stored, storage life is short, and bud rate seed vitality can all be reduced.In constant-temperature and constant humidity store, 15-20
DEG C, under 60% damp condition, can typically store 3-10, during preservation, germination percentage and seed vitality can all be reduced.Although
Holding time is long, but because construction cost is high, general breeding units and breeding team all do not reach condition.
The content of the invention
Deficiency of the present invention for existing Sweet Corn Germplasm resource conservation, there is provided a kind of Sweet Corn Germplasm is preserved and bred more
New method, using the inventive method in the warehouse of general nature condition, can store Sweet Corn Germplasm 3-5, and obtain
Sweet Corn Germplasm its primary characteristic can be kept not change, can be cost-effective and keep germination percentage higher.
To realize above-mentioned target, the technical solution adopted by the present invention is, a kind of Sweet Corn Germplasm is preserved and reproduction and renewal
Method, it is characterized in that, step is as follows：
(1) the Sweet Corn Germplasm self-mating system kind that will be preserved in field, and by female fringe bagging；
(2) genetic background identical conventional corn self-mating system pollen is taken, by pollen pollination in the corn female fringe of bagging；
(3) autumn results pollination fruit ear, obtains corn and conventional corn F1 generation cenospecies；
(4) F1 generation cenospecies is stored in warehouse；
(5) after 3-5, F1 generation cenospecies is taken out, is planted in field, and bagging selfing；
(6) selfing fruit ear is harvested, is dried, select shrinkage seed on fruit ear, obtain final product Sweet Corn Germplasm seed.
The Sweet Corn Germplasm seed and original Sweet Corn Germplasm obtained in the step (6) are planted in field, breeding time jointly
Compare both External Characters consistent.
The Sweet Corn Germplasm includes common corn, super-sweet corn, reinforced corn.
The warehouse is the warehouse of conventional natural draft drying.
The present invention has the advantages that：
1st, corn and conventional corn are hybridized with the inventive method, cenospecies preserves in the warehouse of general nature condition
, 3-5 can be stored.Sweet corn seeds 1-2 is directly preserved relative to original method, the present invention significantly extends corn
The preserving seed time limit.
2nd, the cenospecies preserved in Germplasm Bank, field planting, selfing obtains homozygosis Sweet Corn Germplasm, compared with original germplasm
Its primary characteristic can be kept not change, and keep germination percentage higher.Illustrate that the inventive method is effective, and safety can
Lean on.
3rd, the inventive method can successfully extend the Sweet Corn Germplasm holding time, it is to avoid in the prior art, corn kind
Quality guarantee is deposited unfavorable, it is necessary to the annual shortcoming for all entering reproduction and renewal.While without constant temperature and humidity by need to being only stored in custom library
Storehouse, can be greatly cost-effective.
Brief description of the drawings
Fig. 1 is the preserving seed technology path of super-sweet corn.
Fig. 2 is the preserving seed technology path for strengthening corn.
Fig. 3 is the preserving seed technology path of common corn.
Specific embodiment
Technical scheme and its produced technique effect are carried out with reference to specific test method and accompanying drawing
It is further elucidated above, the description below merely to explain the present invention, but the present invention is any limitation as never in any form, based on this
Any conversion or replacement that invention training centre is made, belong to protection scope of the present invention.
Method used in the present invention unless otherwise specified, is this area conventional method.It is used in following embodiments
Test material be Shandong Crop Germplasm Resource Center Germplasm Bank and preserve.
Embodiment 1
Technology path is as shown in Fig. 2 comprise the following steps that：
In July, (1) 2011, Yuegongqiao Dang Jia farms, super-sweet corn self-mating system TX01 and genetic background identical are common
Various two rows of corn inbred line LX9801, row is long 4.5 meters, 60 centimetres of line-spacing, 4000 plants every mu of density.
(2) super-sweet corn self-mating system TX01 selects 20 plants by female fringe bagging.The pollen of conventional corn self-mating system LX9801 is taken, will
Pollen is awarded in 10 plants of female fringes of bagging.Other 10 plants of super-sweet corns self-mating system bagging selfing.
In by the end of September, (3) 2012, harvests the fruit ear of bagging pollination.By pollinate conventional corn self-mating system 10 fruit ears we
Be named as F1,10 fruit ears of bagging selfing we be named as S1.Each 10 fruit ears of F1, S1 are dried into threshing, by two kinds of seeds
Select respectively it is full without small holes caused by worms without downright bad seed, be attached separately in paper bag, in paper bag respectively load two camphor balls, be stored in warehouse
In.Warehouse keeps drying, natural temperature.
In June, (4) 2015, take out and preserve two kinds of seeds, plant respectively in the row of field two.Farm is located at Zhangqiu Dang Jia towns, field
Between row it is long 4.5 meters, 60 centimetres of line-spacing, 4000 plants every mu of density.Field investigation bud ratio, F1 percentages of seedgermination are 69%, S1 kinds
Sub- germination percentage is 13%.The row corn of F1, S1 two is respectively selected into 10 plants of bagging selfings.F1 fecundities are better than S1.In by the end of September, 2015, receives
Obtain the fringe of F1 bagging selfings fruit ear 10.Fruit ear is dried, shrinkage seed on fruit ear is selected.
In June, (5) 2016 will select shrinkage seed and each two rows kinds of super-sweet corn self-mating system TX01 in field.Breeding time ratio
It is consistent compared with both External Characters.
Embodiment 2
Technology path is as shown in figure 3, comprise the following steps that：
In July, (1) 2010, Yuegongqiao Dang Jia farms, strengthen inbred line of sweet corn neat 501 and genetic background identical is general
Logical corn inbred line various two rows of LX9801, row is long 4.5 meters, 60 centimetres of line-spacing, 4000 plants every mu of density.
(2) strengthen inbred line of sweet corn neat 501 and select 20 plants by female fringe bagging.Take the flower of conventional corn self-mating system LX9801
Powder, pollen is awarded in 10 plants of female fringes of bagging.Other 10 plants of reinforcements inbred line of sweet corn bagging selfing.
In by the end of September, (3) 2012, harvests the fruit ear of bagging pollination.By pollinate conventional corn self-mating system 10 fruit ears we
Be named as F1,10 fruit ears of bagging selfing we be named as S1.Each 10 fruit ears of F1, S1 are dried into threshing, by two kinds of seeds
Select respectively it is full without small holes caused by worms without downright bad seed, be attached separately in paper bag, in paper bag respectively load two camphor balls, be stored in warehouse
In.Warehouse keeps drying, natural temperature.
In June, (4) 2015, take out and preserve two kinds of seeds, plant respectively in the row of field two.Farm is located at Zhangqiu Dang Jia towns, field
Between row it is long 4.5 meters, 60 centimetres of line-spacing, 4000 plants every mu of density.Field investigation bud ratio, F1 percentages of seedgermination are 23%, S1 kinds
Sub- germination percentage is 0%.In by the end of September, 2015, harvests the fringe of F1 bagging selfings fruit ear 10.Fruit ear is dried, shrinkage seed on fruit ear is selected
Grain.
In June, (5) 2016 will select shrinkage seed and strengthen each two rows kind of inbred line of sweet corn neat 501 in field.Fertility
It is consistent that phase compares both External Characters.
Embodiment 3
Technology path is as shown in figure 1, comprise the following steps that：
In July, (1) 2010, the farm of Jinan Qihe 61, common inbred line of sweet corn TX1001 and conventional corn self-mating system
Various two rows of LX9801, row is long 5 meters, 66.7 centimetres of line-spacing, 4000 plants every mu of density.
(2) common inbred line of sweet corn TY001 selects 20 plants by female fringe bagging.Take the flower of conventional corn self-mating system LX9801
Powder, pollen is awarded in 10 plants of female fringes of bagging.Other 10 plants common inbred line of sweet corn bagging selfings.
At the beginning of 10 months (3) 2010 years, the fruit ear of bagging pollination is harvested.By pollinate conventional corn self-mating system 10 fruit ears we
Be named as F1,10 fruit ears of bagging selfing we be named as S1.Each 10 fruit ears of F1, S1 are dried into threshing, by two kinds of seeds
Select respectively it is full without small holes caused by worms without downright bad seed, be attached separately in paper bag, in paper bag respectively load two camphor balls, be stored in warehouse
In.Warehouse keeps drying, natural temperature.
In June, (4) 2013, take out and preserve two kinds of seeds, plant respectively in the row of field two.Farm is located at Zhangqiu Dang Jia towns, field
Between row it is long 5 meters, 60 centimetres of line-spacing, 4000 plants every mu of density.F1 percentages of seedgermination are that 87%, S1 percentages of seedgermination are 25%.Will
The row corn of F1, S1 two respectively selects 10 plants of bagging selfings.F1 fecundities are better than S1.In by the end of September, 2013, harvests F1 bagging selfings fruit ear 10
Fringe.Fruit ear is dried, shrinkage seed on fruit ear is selected.
In June, (5) 2014 will select shrinkage seed and each two rows kinds of common inbred line of sweet corn TX1001 in field.Fertility
It is consistent that phase compares both External Characters.
Claims (4)
1. a kind of Sweet Corn Germplasm preserve and reproduction and renewal method, it is characterized in that, step is as follows：
(1) the Sweet Corn Germplasm self-mating system kind that will be preserved in field, and by female fringe bagging；
(2) genetic background identical conventional corn self-mating system pollen is taken, by pollen pollination in the corn female fringe of bagging；
(3) autumn results pollination fruit ear, obtains corn and conventional corn F1 generation cenospecies；
(4) F1 generation cenospecies is stored in warehouse；
(5) after 3-5, F1 generation cenospecies is taken out, is planted in field, and bagging selfing；
(6) selfing fruit ear is harvested, is dried, select shrinkage seed on fruit ear, obtain final product Sweet Corn Germplasm seed.
2. Sweet Corn Germplasm as claimed in claim 1 preserve and reproduction and renewal method, it is characterized in that, in the step (6)
The Sweet Corn Germplasm seed and original Sweet Corn Germplasm of acquisition are planted in field jointly, and it is consistent that breeding time compares both External Characters.
3. Sweet Corn Germplasm as claimed in claim 1 or 2 preserve and reproduction and renewal method, it is characterized in that, the corn
Germplasm includes common corn, super-sweet corn, reinforced corn.
4. Sweet Corn Germplasm as claimed in claim 1 or 2 preserve and reproduction and renewal method, it is characterized in that, the warehouse is
The warehouse of conventional natural draft drying.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611069963.2A CN106718829A (en) | 2016-11-28 | 2016-11-28 | A kind of Sweet Corn Germplasm preservation and the method for reproduction and renewal |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201611069963.2A CN106718829A (en) | 2016-11-28 | 2016-11-28 | A kind of Sweet Corn Germplasm preservation and the method for reproduction and renewal |
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| CN201611069963.2A Pending CN106718829A (en) | 2016-11-28 | 2016-11-28 | A kind of Sweet Corn Germplasm preservation and the method for reproduction and renewal |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114698631A (en) * | 2022-04-25 | 2022-07-05 | 青岛金妈妈农业科技有限公司 | Corn pollen storage method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104472339A (en) * | 2014-11-05 | 2015-04-01 | 温州市农业科学研究院作物研究所 | Breeding method for high-yield pest-resistant sweet corn |
| US20150296732A1 (en) * | 2014-04-22 | 2015-10-22 | Seminis Vegetable Seeds, Inc. | Sweet corn hybrid sv1408sa and parents thereof |
| CN105075856A (en) * | 2015-09-25 | 2015-11-25 | 云南省农业科学院粮食作物研究所 | Method for breeding double recessive homozygous sweet-waxy maize hybrid through sh2 gene type super-sweet maize mutant with high starch content |
| CN105850712A (en) * | 2016-04-05 | 2016-08-17 | 广东省农业科学院作物研究所 | Breeding method of strong-superiority hybrid seeds of super-sweet corn |
-
2016
- 2016-11-28 CN CN201611069963.2A patent/CN106718829A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20150296732A1 (en) * | 2014-04-22 | 2015-10-22 | Seminis Vegetable Seeds, Inc. | Sweet corn hybrid sv1408sa and parents thereof |
| CN104472339A (en) * | 2014-11-05 | 2015-04-01 | 温州市农业科学研究院作物研究所 | Breeding method for high-yield pest-resistant sweet corn |
| CN105075856A (en) * | 2015-09-25 | 2015-11-25 | 云南省农业科学院粮食作物研究所 | Method for breeding double recessive homozygous sweet-waxy maize hybrid through sh2 gene type super-sweet maize mutant with high starch content |
| CN105850712A (en) * | 2016-04-05 | 2016-08-17 | 广东省农业科学院作物研究所 | Breeding method of strong-superiority hybrid seeds of super-sweet corn |
Non-Patent Citations (1)
| Title |
|---|
| 朱良骅等: "甜玉米育种若干问题的商讨", 《河南农业科学》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114698631A (en) * | 2022-04-25 | 2022-07-05 | 青岛金妈妈农业科技有限公司 | Corn pollen storage method |
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Application publication date: 20170531 |
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