CN106701721A - Technological method for extracting lumbrokinase by utilizing living earthworms - Google Patents
Technological method for extracting lumbrokinase by utilizing living earthworms Download PDFInfo
- Publication number
- CN106701721A CN106701721A CN201611158370.3A CN201611158370A CN106701721A CN 106701721 A CN106701721 A CN 106701721A CN 201611158370 A CN201611158370 A CN 201611158370A CN 106701721 A CN106701721 A CN 106701721A
- Authority
- CN
- China
- Prior art keywords
- earthworm
- lumbrokinase
- extracts
- bed
- live body
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6435—Plasmin (3.4.21.7), i.e. fibrinolysin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21007—Plasmin (3.4.21.7), i.e. fibrinolysin
Abstract
The invention provides a technological method for extracting lumbrokinase by utilizing living earthworms. The technological method comprises the following technological steps: collecting the earthworms, extracting the lumbrokinase, precipitating, centrifuging, sterilizing and drying and pulverizing. The extracted lumbrokinase can be used for promoting the increasing of the quantity of the earthworms and microorganisms in soil; meanwhile, the soil can be effectively activated and loosened and the content of organic matters in the soil is increased; a soil structure and physicochemical properties are improved, and absorption of root systems on nutrients is facilitated.
Description
Technical field
The present invention relates to the process that a kind of utilization live body earthworm extracts Lumbrokinase.
Background technology
China is fertilizer application big country, but this season utilization rate of chemical fertilizer is very low.At present, China's utilization rate of nitrogen fertilizer is about
30%~35%, chemical fertilizer utilization ratio is low, not only wastes resource, increased peasant's input.And also result in environmental pollution, phosphorus
The discharge of gypsum, high phosphorus waste water and fluoro-gas high also brings very big pressure to environment.Next to that resource pressure.Production chemical fertilizer
Resource such as phosphorus ore, sylvite, coal and natural gas etc. be all non-renewable resources, how to realize not only having economized on resources but also having met increasingly
The Fertilizer demand of growth is a very big challenge for Fertilizer Industry in China.
Lumbrokinase is a kind of organic substance, can promote the increase of earthworm amount and microbial biomass in soil, while can have
The activation of effect, chesson increases the content of soil with organic matter, improves soil texture and physicochemical property, promotes root system to supporting
The absorption for dividing.
The content of the invention：
To improve utilization rate of fertilizer, the hardened phenomenon of improved soil, the invention provides a kind of Lumbrokinase preparation method, is prepared
Lumbrokinase can apply in fertilizer.
Innovative point of the invention is to extract the whole of Lumbrokinase all to use live body earthworm.
The present invention is achieved through the following technical solutions：
The collection of earthworm：The fresh and alive earthworm of same day collection is taken, is cleaned with warm water.
The extraction of Lumbrokinase：Clean earthworm thin layer is layered in extraction bed (network structure), is extracted above bed
Mobile fog-spray nozzle can stimulate the material of earthworm body secretes Lumbrokinase every 5-10min to sprinkling on bed is extracted, complete to earthworm
It is complete dead.
Precipitation：The extract solution below extraction bed is taken out, protein precipitant is added, stood.
Centrifugation：By the extract solution containing precipitation obtained by above-mentioned steps be placed in the supercentrifuge of rotating speed 10000r/s from
Heart 10-15min.
It is sterilized：Sediment fraction after centrifugation is placed under uviol lamp and irradiates 1~2h.
Drying powder processed：It is put into through the precipitation after sterilization and is dried in aseptic vacuum freeze drier 5-8 hours, is obtained earthworm and swash
Enzyme dry powder.
Specific embodiment：
The process that live body earthworm of the present invention extracts Lumbrokinase is specifically described below in conjunction with example.
The collection of earthworm：The fresh and alive earthworm that same day collection is sent, after removal soil, is gently cleaned, earthworm with warm water
Body surface is cleaned up.
The extraction of Lumbrokinase：The clean earthworms of 100kg are weighed, very thin one layer is layered in extraction bed (network structure), carries
Taking the mobile fog-spray nozzle above bed can stimulate the material of earthworm body secretes Lumbrokinase every 5-10min to sprinkling on bed is extracted
It is completely dead to earthworm.
Precipitation：The extract solution below extraction bed is taken out, protein precipitant is added, stood.
Centrifugation：By the extract solution containing precipitation obtained by above-mentioned steps be placed in the supercentrifuge of rotating speed 10000r/s from
Heart 10-15min.
It is sterilized：Sediment fraction after centrifugation is placed under uviol lamp and irradiates 1~2h.
Drying powder processed：It is put into through the precipitation after sterilization and is dried in aseptic vacuum freeze drier 5-8 hours, is obtained earthworm and swash
Enzyme dry powder.
Although the present specification is described in terms of embodiments, not each implementation method only includes an independent skill
Art scheme, this narrating mode of specification is only for clarity, it is intended that the equivalency that will fall in claim
Implication and scope in all changes include in the present invention.
Claims (4)
1. a kind of utilization live body earthworm extracts the process of Lumbrokinase, it is characterised in that comprise the following steps：
1) collection of earthworm：The fresh and alive earthworm of same day collection is taken, is cleaned with warm water.
2) extraction of Lumbrokinase：Clean earthworm thin layer is layered in extraction bed (network structure), the shifting above bed is extracted
Dynamic fog-spray nozzle can stimulate the material of earthworm body secretes Lumbrokinase every 5-10min to sprinkling on bed is extracted, complete to earthworm
It is dead.
3) precipitate：The extract solution below extraction bed is taken out, protein precipitant is added, stood.
4) it is centrifuged：The extract solution containing precipitation obtained by above-mentioned steps is placed in the supercentrifuge of rotating speed 10000r/s and is centrifuged
10-15min。
5) it is sterilized：Sediment fraction after centrifugation is placed under uviol lamp and irradiates 1~2h.
6) drying powder processed：It is put into through the precipitation after sterilization and is dried in aseptic vacuum freeze drier 5-8 hours, obtains Lumbrokinase
Dry powder.
2. a kind of utilization live body earthworm as claimed in claim 1 extracts the process of Lumbrokinase, it is characterised in that the step
Rapid 1) collection is new living body earthworm.
3. a kind of utilization live body earthworm as claimed in claim 1 extracts the process of Lumbrokinase, it is characterised in that the step
2) rapid be to use live body earthworm.
4. a kind of utilization live body earthworm as claimed in claim 1 extracts the process of Lumbrokinase, it is characterised in that the step
The material of rapid 2) middle sprinkling includes all materials that can stimulate and Lumbrokinase is secreted in earthworm body.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611158370.3A CN106701721A (en) | 2016-12-08 | 2016-12-08 | Technological method for extracting lumbrokinase by utilizing living earthworms |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611158370.3A CN106701721A (en) | 2016-12-08 | 2016-12-08 | Technological method for extracting lumbrokinase by utilizing living earthworms |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106701721A true CN106701721A (en) | 2017-05-24 |
Family
ID=58937733
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611158370.3A Pending CN106701721A (en) | 2016-12-08 | 2016-12-08 | Technological method for extracting lumbrokinase by utilizing living earthworms |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106701721A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109628324A (en) * | 2018-12-29 | 2019-04-16 | 天津天丰泽田生物科技有限公司 | A kind of bioremediation agents and preparation method of cadmium pollution |
| CN109735345A (en) * | 2018-12-29 | 2019-05-10 | 天津天丰泽田生物科技有限公司 | A kind of compound biological enzyme heavy metal-polluted soil renovation agent and preparation method |
| CN109749750A (en) * | 2018-12-27 | 2019-05-14 | 天津天丰泽田生物科技有限公司 | A kind of heavy metal pollution of soil renovation agent and preparation method thereof |
| CN110403186A (en) * | 2018-04-26 | 2019-11-05 | 王鹏 | A kind of earthworm zymolysis technique |
| CN112646586A (en) * | 2020-12-11 | 2021-04-13 | 广东省科学院生态环境与土壤研究所 | Method for enhancing stability of organic carbon in soil |
-
2016
- 2016-12-08 CN CN201611158370.3A patent/CN106701721A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110403186A (en) * | 2018-04-26 | 2019-11-05 | 王鹏 | A kind of earthworm zymolysis technique |
| CN109749750A (en) * | 2018-12-27 | 2019-05-14 | 天津天丰泽田生物科技有限公司 | A kind of heavy metal pollution of soil renovation agent and preparation method thereof |
| CN109628324A (en) * | 2018-12-29 | 2019-04-16 | 天津天丰泽田生物科技有限公司 | A kind of bioremediation agents and preparation method of cadmium pollution |
| CN109735345A (en) * | 2018-12-29 | 2019-05-10 | 天津天丰泽田生物科技有限公司 | A kind of compound biological enzyme heavy metal-polluted soil renovation agent and preparation method |
| CN112646586A (en) * | 2020-12-11 | 2021-04-13 | 广东省科学院生态环境与土壤研究所 | Method for enhancing stability of organic carbon in soil |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170524 |