CN106699836A - Dialysis equipment for protein renaturation - Google Patents
Dialysis equipment for protein renaturation Download PDFInfo
- Publication number
- CN106699836A CN106699836A CN201710131276.7A CN201710131276A CN106699836A CN 106699836 A CN106699836 A CN 106699836A CN 201710131276 A CN201710131276 A CN 201710131276A CN 106699836 A CN106699836 A CN 106699836A
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- CN
- China
- Prior art keywords
- container
- renaturation
- dialysis
- equipment according
- liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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- 238000004153 renaturation Methods 0.000 title claims abstract description 58
- 238000000502 dialysis Methods 0.000 title claims abstract description 39
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 14
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 14
- 238000001816 cooling Methods 0.000 claims abstract description 16
- 239000007788 liquid Substances 0.000 claims description 24
- 238000009413 insulation Methods 0.000 claims description 15
- 238000005259 measurement Methods 0.000 claims description 7
- 238000012544 monitoring process Methods 0.000 claims description 5
- 238000004891 communication Methods 0.000 claims description 4
- 230000000694 effects Effects 0.000 abstract description 8
- 229960000074 biopharmaceutical Drugs 0.000 abstract description 2
- 238000000034 method Methods 0.000 description 5
- 210000003000 inclusion body Anatomy 0.000 description 3
- 229960000789 guanidine hydrochloride Drugs 0.000 description 2
- PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 description 2
- 230000002572 peristaltic effect Effects 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 1
- 108090000386 Fibroblast Growth Factor 1 Proteins 0.000 description 1
- 102100031706 Fibroblast growth factor 1 Human genes 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000001139 pH measurement Methods 0.000 description 1
- 238000009738 saturating Methods 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Water Supply & Treatment (AREA)
- External Artificial Organs (AREA)
Abstract
The invention belongs to the field of biopharmaceutical equipment, and particularly relates to dialysis equipment for protein renaturation. The dialysis equipment comprises a container accommodating a renaturation solution, wherein a cooling insulating layer is attached to outside of the container. The cooling insulating layer is attached to the outside of the container and can cool the renaturation solution in the container persistently, so that the dialysis renaturation temperature is guaranteed, dialysis renaturation effect is guaranteed, meanwhile, dialysis renaturation operation can be simplified, and dialysis renaturation efficiency is improved.
Description
Technical field
The invention belongs to bio-pharmaceuticals apparatus field, more particularly to a kind of dialysis machine of protein renaturation.
Background technology
Escherichia coli generally express exogenous proteins with inclusion bodies, and the protein in inclusion body is in denatured state,
Need to carry out renaturation process to it that bioactivity could be obtained.The refolding method commonly used in production at present is dialysis renaturation, with height
Concentration guanidine hydrochloride dissolution inclusion body, is sealed against in large volume renaturation solution or dialyzate in bag filter, is subsequently placed in, using saturating
The semi-transparent film character of bag is analysed, guanidine hydrochloride progressively can be discharged into renaturation solution from bag filter, and protein is able to renaturation in the process.
When dialysis renaturation operation is carried out, renaturation solution configuration needs to be placed in precooling in refrigerator or low temperature workshop after finishing, and takes time and effort, and dialyses
Efficiency is low, and dialysis when renaturation solution temperature it is uncontrollable, cause dialysis degree uncontrollable.
The content of the invention
It is not required to carry out precooling to renaturation solution it is an object of the invention to provide one kind, can effectively controls dialysis renaturation temperature
The dialysis machine of protein renaturation.
To realize object above, the technical solution adopted by the present invention is：A kind of dialysis machine of protein renaturation, including hold
Receive the container of renaturation solution, described external container is attached to cooling heat-insulation layer.
Compared with prior art, there is following technique effect in the present invention：The present invention sets up cooling heat-insulation layer in external container,
Constantly the renaturation solution in container can be cooled down using heat-insulation layer is cooled down, so as to ensure dialysis renaturation temperature, entered
And ensure dialysis renaturation effect, while dialysis renaturation operation can also be simplified, improve dialysis renaturation efficiency.
Brief description of the drawings
The content expressed by each width accompanying drawing of this specification and the mark in figure are briefly described below：
Fig. 1 is structural representation of the invention；
Fig. 2 is the structural representation of hanger of the present invention and Dialysis container.
In figure：10. container, 11. cavity volumes, 111. inlets, 112. liquid outlets, 12. liquid pumps, 121. entrances, 122. outlets,
13. hangers, 14. monitoring alarms, 20. outside plates, 21. folder chambers, 30. heat-insulation layers, 40. Dialysis containers.
Specific embodiment
Below in conjunction with the accompanying drawings 1,2, by the description to embodiment, specific embodiment of the invention is made further in detail
Explanation.
A kind of dialysis machine of protein renaturation, including the container 10 of renaturation solution is accommodated, set up outside described container 10
There is cooling heat-insulation layer 30.Cooling heat-insulation layer 30 can be cooled down constantly to the renaturation solution in container 10, so as to ensure
Dialysis renaturation temperature, and then ensure dialysis renaturation effect, while also simplify dialysis renaturation operation, improve dialysis renaturation effect
Rate.The equipment can be used for the dialysis renaturation of the protein such as IFN, KGF, EGF, aFGF, it is also possible to for the renaturation of other protein.
The various structures of heat-insulation layer 30 are cooled down, here preferably, described cooling heat-insulation layer 30 includes being arranged on container 10
Outside outside plate 20, the folder chamber 21 between outside plate 20 and container 10 is connected with low-temperature receiver, enables low-temperature receiver in folder chamber 21 and container
Renaturation solution in 10 carries out fully heat exchange, it is ensured that cooling effect.Low-temperature receiver should be according to renaturation requirement selected its type and cooling
Temperature, is dialysed with the renaturation for adapting to different proteins.In the present embodiment preferably, using flow-like low-temperature receiver, folder chamber 21 is by pipe
Road connects circulating pump and forms circulation loop therewith, and the circulating pump freezes low-temperature receiver and for its circulating in circulation loop carries
For power, to ensure the cooling effect of stabilization.Further, it is attached to heat-insulation layer 30 outside described outside plate 20, it is to avoid low-temperature receiver
Consumption, improve cooling heat-insulation layer 30 cooling effectiveness.
Preferably, the container 10 is provided with the online inlet 111 and liquid outlet 112 for changing liquid, is easy to renaturation solution more
Change, it is easy to operate, quick, annealing efficiency can be effectively improved.It is stroke of the increase renaturation solution in cavity volume 11, lifting renaturation solution
Utilization ratio, inlet 111 is located at the first half of container 10, and liquid outlet 112 is located at the cavity bottom of container 10.
Similarly, in order to accelerate dialysis renaturation speed, here preferably, also including ordering about cavity volume of the renaturation solution in container 10
Convection current or circulated in 11, entrance 121 and the outlet 122 of the mixed uniformly liquid pump 12 of renaturation liquid energy, liquid pump 12 is submerged multiple
In property liquid and away from being arranged in cavity volume 11.Renaturation solution is pumped into liquid pump 12 from entrance 121 and pumped out from outlet 122, it is ensured that multiple
Property liquid be fully contacted with Dialysis container 40 in the cavity volume 11, and the uniformity of renaturation solution concentration can be improved.The number of liquid pump 12
Amount and its entrance 121, the arrangement of outlet 122 should be adjusted according to the volume of container 10, to ensure the uniform mixing of renaturation solution.
Liquid pump 12 can select peristaltic pump or immersible pump, and during from peristaltic pump, the pump line of renaturation solution Scrape pump does not contact pump
Body, can be effectively ensured the cleaning of renaturation solution；During from immersible pump, the immersible pump pump housing is arranged in container 10 using sucker
On wall, install convenient is easy to the adjustment of its quantity and installation site.
Preferably, in order to prevent the Dialysis container 40 for accommodating denaturing liquid from being collided in whipping process, Dialysis container is made
40 seal failure, causes the loss that denaturing liquid is unnecessary, and the liquid level of renaturation solution is provided with hanger 13 in described container 10, hangs
The underhung of frame 13 has the Dialysis container 40 for accommodating denaturing liquid.In the present embodiment preferably, hanger 13 rides upon the top of container 10,
Dialysis container 40 is suspended on the lower section of hanger 13 with soft rope, has both enable the free movement within the specific limits of Dialysis container 40, prevents again
Dialysis container 40 occurs more violent collision causes rupture, and dialytic efficiency is effectively ensured.
Preferably, monitoring alarm 14 is provided with described container 10, monitoring alarm 14 includes measurement sensor,
Measurement sensor is electrically connected with communication module.Measurement sensor can from pH measurement sensors, conductivity measurement sensors and
Temperature sensor etc., when the measurement data such as pH, electrical conductivity, temperature exceed predetermined scope, communication module is to the reception for setting
Equipment carries out communication alert, so as to ensure dialysis renaturation effect.
Preferably, described cooling heat-insulation layer 30 bottom is provided with the roller for being easy to equipment to move, to realize the facility of equipment
Using.
Claims (8)
1. a kind of dialysis machine of protein renaturation, including the container (10) for accommodating renaturation solution, it is characterised in that：Described container
(10) it is outside to be attached to cooling heat-insulation layer.
2. equipment according to claim 1, it is characterised in that：Described cooling heat-insulation layer includes being arranged on container (10) outward
The outside plate (20) in portion, the folder chamber (21) between outside plate (20) and container (10) is connected with low-temperature receiver.
3. equipment according to claim 2, it is characterised in that：Heat-insulation layer (30) is attached to outside described outside plate (20).
4. equipment according to claim 1, it is characterised in that：The container (10) is provided with the online inlet for changing liquid
(111) and liquid outlet (112), positioned at the first half of container (10), liquid outlet is located at the chamber bottom of container (10) to inlet (111)
Portion.
5. the equipment according to claim 1 or 4, it is characterised in that：Also include ordering about cavity volume of the renaturation solution in container (10)
(11) liquid pump (12) of internal circulation flow, the entrance (121) of liquid pump (12) and outlet (122) are submerged in renaturation solution and away from arrangement
In cavity volume (11).
6. equipment according to claim 1, it is characterised in that：The liquid level of described container (10) interior renaturation solution is provided with extension
Frame (13), hanger (13) underhung has the Dialysis container (40) for accommodating denaturing liquid.
7. equipment according to claim 1, it is characterised in that：Monitoring alarm is provided with described container (10)
(14), monitoring alarm (14) includes measurement sensor, and measurement sensor is electrically connected with communication module.
8. equipment according to claim 1, it is characterised in that：Described cooling heat-insulation layer bottom is provided with is easy to equipment to move
Roller.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710131276.7A CN106699836A (en) | 2017-03-07 | 2017-03-07 | Dialysis equipment for protein renaturation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710131276.7A CN106699836A (en) | 2017-03-07 | 2017-03-07 | Dialysis equipment for protein renaturation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106699836A true CN106699836A (en) | 2017-05-24 |
Family
ID=58918025
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710131276.7A Pending CN106699836A (en) | 2017-03-07 | 2017-03-07 | Dialysis equipment for protein renaturation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106699836A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109316969A (en) * | 2018-10-29 | 2019-02-12 | 佛山市铁人环保科技有限公司 | A kind of purifier apparatus and production process control method of silica solution fertilizer |
| CN109336949A (en) * | 2018-11-30 | 2019-02-15 | 天津威特生物医药有限责任公司 | Protein renaturation reaction unit and reaction method |
| CN110922449A (en) * | 2018-09-20 | 2020-03-27 | 江苏健安生物科技有限公司 | Automatic insoluble recombinant protein resuscitation device |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4933434A (en) * | 1986-04-08 | 1990-06-12 | Boehringer Mannheim Gmbh | Process for obtaining renatured proteins |
| CN202415421U (en) * | 2011-12-29 | 2012-09-05 | 郑州伊美诺生物技术有限公司 | Simple dynamic protein dialysis system |
| CN203238200U (en) * | 2013-04-19 | 2013-10-16 | 狄建军 | Protein dialysis device |
| CN205152131U (en) * | 2015-10-24 | 2016-04-13 | 湖北省银丰鼎诚生物工程有限公司 | Portable nickel post auxiliary device |
| CN206705999U (en) * | 2017-03-07 | 2017-12-05 | 安徽鑫华坤生物工程有限公司 | A kind of dialysis machine of protein renaturation |
-
2017
- 2017-03-07 CN CN201710131276.7A patent/CN106699836A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4933434A (en) * | 1986-04-08 | 1990-06-12 | Boehringer Mannheim Gmbh | Process for obtaining renatured proteins |
| CN202415421U (en) * | 2011-12-29 | 2012-09-05 | 郑州伊美诺生物技术有限公司 | Simple dynamic protein dialysis system |
| CN203238200U (en) * | 2013-04-19 | 2013-10-16 | 狄建军 | Protein dialysis device |
| CN205152131U (en) * | 2015-10-24 | 2016-04-13 | 湖北省银丰鼎诚生物工程有限公司 | Portable nickel post auxiliary device |
| CN206705999U (en) * | 2017-03-07 | 2017-12-05 | 安徽鑫华坤生物工程有限公司 | A kind of dialysis machine of protein renaturation |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110922449A (en) * | 2018-09-20 | 2020-03-27 | 江苏健安生物科技有限公司 | Automatic insoluble recombinant protein resuscitation device |
| CN109316969A (en) * | 2018-10-29 | 2019-02-12 | 佛山市铁人环保科技有限公司 | A kind of purifier apparatus and production process control method of silica solution fertilizer |
| CN109336949A (en) * | 2018-11-30 | 2019-02-15 | 天津威特生物医药有限责任公司 | Protein renaturation reaction unit and reaction method |
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| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170524 |
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| RJ01 | Rejection of invention patent application after publication |