CN110922449A - Automatic insoluble recombinant protein resuscitation device - Google Patents
Automatic insoluble recombinant protein resuscitation device Download PDFInfo
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- CN110922449A CN110922449A CN201811098071.4A CN201811098071A CN110922449A CN 110922449 A CN110922449 A CN 110922449A CN 201811098071 A CN201811098071 A CN 201811098071A CN 110922449 A CN110922449 A CN 110922449A
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- dialysis
- protein
- recombinant protein
- dialysis tube
- jar
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- 102000007056 Recombinant Fusion Proteins Human genes 0.000 title claims abstract description 44
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 title claims abstract description 44
- 238000000502 dialysis Methods 0.000 claims abstract description 134
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 72
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 71
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 46
- 239000012528 membrane Substances 0.000 claims abstract description 12
- 239000012780 transparent material Substances 0.000 claims abstract description 4
- 239000002351 wastewater Substances 0.000 claims description 7
- 239000000758 substrate Substances 0.000 claims description 6
- 238000011084 recovery Methods 0.000 abstract description 14
- 239000012460 protein solution Substances 0.000 abstract description 3
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 16
- 239000004202 carbamide Substances 0.000 description 16
- 239000000243 solution Substances 0.000 description 14
- 239000003398 denaturant Substances 0.000 description 8
- 230000008859 change Effects 0.000 description 7
- 238000000034 method Methods 0.000 description 6
- 230000008569 process Effects 0.000 description 5
- 210000003000 inclusion body Anatomy 0.000 description 3
- 238000001556 precipitation Methods 0.000 description 3
- 241000588724 Escherichia coli Species 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 229960000789 guanidine hydrochloride Drugs 0.000 description 2
- PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 230000006920 protein precipitation Effects 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 239000003638 chemical reducing agent Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000004886 process control Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 210000001236 prokaryotic cell Anatomy 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000003716 rejuvenation Effects 0.000 description 1
- 238000004153 renaturation Methods 0.000 description 1
- 238000001226 reprecipitation Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000000870 ultraviolet spectroscopy Methods 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/30—Extraction; Separation; Purification by precipitation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
Abstract
The invention relates to an automatic insoluble recombinant protein recovery device, which comprises a dialysis tank, wherein a protein dialysis tube made of transparent materials is arranged at the top of the dialysis tank, the bottom end of the protein dialysis tube penetrates through the top wall of the dialysis tank and extends into the inner cavity of the dialysis tank, a dialysis membrane is arranged on the protein dialysis tube positioned in the inner cavity of the dialysis tank, the inner side and the outer side of the dialysis membrane are respectively communicated with the inner cavity of the protein dialysis tube and the inner cavity of the dialysis tank, a light source and a photosensitive device are arranged on the outer side of the protein dialysis tube positioned above the top wall of the dialysis tank, the automatic insoluble recombinant protein recovery device further comprises a water pump and a controller, the water outlet end of the water pump is communicated with the dialysis tank through a pipeline, and the controller is connected with the electric input end of the water pump and the output end of the photosensitive device in. The automatic insoluble recombinant protein recovery device can automatically control the water flow and the water flow speed according to the turbidity feedback of the recombinant protein solution.
Description
Technical Field
The invention relates to a recombinant protein recovery device, in particular to an automatic insoluble recombinant protein recovery device.
Background
Biologically active proteins expressed by cells often exist in the form of a meltable or molecular complex, whereas proteins that are functional for expression always fold into a specific three-dimensional structure. When the foreign gene is expressed in prokaryotic cells (e.g., E.coli), the expressed recombinant protein is usually present in the form of insoluble protein in inclusion bodies. Inclusion bodies are high density, insoluble protein particles encapsulated by membranes formed when efficiently expressed in e.coli. The proteins in inclusion bodies are aggregates in an unfolded state and generally have no biological activity, so that the recombinant proteins need to be recovered or renatured before use. So-called recovery, i.e. after the protein is dissolved again by using the denaturant, the denaturant is slowly removed to restore the target protein from the denatured fully-extended state to a normal folded structure, and simultaneously the reducing agent is removed to enable the normal formation of disulfide bonds. Commonly used denaturants include guanidine hydrochloride, urea, ammonium sulfate, and the like. In the case of urea, the protein is generally first dissolved in 8M urea. The urea concentration in the solution was then reduced stepwise by dialysis. Many proteins begin to renature at urea concentrations around 4M and end up at around 2M. If guanidine hydrochloride is used, the renaturation process can be started from 4M and ended at 1.5M. At present, the process of resuscitating the recombinant protein is mainly carried out by manual liquid changing and a concentration stage gradient dialysis method, and for this reason, a large amount of dialysate containing denaturants with different concentrations needs to be prepared and wasted. The recovery process of the recombinant protein is controlled, and the operation process is complicated. Also, because dialysis is usually large-span, gradient-type, process control for rejuvenation is also very inaccurate.
In view of the above-mentioned drawbacks, the present designer is actively making research and innovation to create an automatic insoluble recombinant protein resuscitating device with a novel structure, so that the device has industrial value.
Disclosure of Invention
In order to solve the technical problems, the invention aims to provide an automatic insoluble recombinant protein recovery device which can automatically control the water flow and the water velocity according to the turbidity feedback of a recombinant protein solution.
The automatic insoluble recombinant protein recovery device comprises a dialysis tank, wherein a protein dialysis tube made of transparent materials is arranged at the top of the dialysis tank, the bottom end of the protein dialysis tube penetrates through the top wall of the dialysis tank and extends into the inner cavity of the dialysis tank, a dialysis membrane is arranged on the protein dialysis tube in the inner cavity of the dialysis tank, the inner side and the outer side of the dialysis membrane are respectively communicated with the inner cavity of the protein dialysis tube and the inner cavity of the dialysis tank, a light source and a photosensitive device are arranged on the outer side of the protein dialysis tube above the top wall of the dialysis tank, the automatic insoluble recombinant protein recovery device further comprises a water pump and a controller, the water outlet end of the water pump is communicated with the dialysis tank through a pipeline, and the controller is connected with the electric input end of the water pump and the output end of the photosensitive device in a wired or wireless connection mode.
Furthermore, according to the automated insoluble recombinant protein resuscitation device, a positioning flange is arranged on the outer side wall of the protein dialysis tube.
Furthermore, according to the automated insoluble recombinant protein resuscitation device, the photosensitive device and the light source are respectively located on the left side and the right side of the protein dialysis tube.
Furthermore, according to the automatic insoluble recombinant protein recovery device, a magnetic stirrer is arranged below the dialysis tank.
Furthermore, the top wall of the protein dialysis tube is provided with a stirrer, the body of the stirrer is positioned on the top wall of the protein dialysis tube, and an output shaft of the stirrer penetrates through the protein dialysis tube and extends into the protein dialysis tube.
Furthermore, the automatic insoluble recombinant protein resuscitation device also comprises a water storage tank, and the water inlet end of the water pump is communicated with the inner cavity of the water storage tank through a pipeline.
Furthermore, the automatic insoluble recombinant protein resuscitation device further comprises a waste water tank, and the inner cavity of the waste water tank is communicated with the inner cavity of the dialysis tank through a pipeline.
Further, according to the automatic insoluble recombinant protein resuscitation device, the light source is an ultraviolet lamp, the photosensitive device is a photosensitive resistor, the water pump is a stepping pump, the controller comprises a substrate and a single chip microcomputer arranged on the substrate, and a port of the single chip microcomputer is connected with the photosensitive resistor and an electrical input end of the stepping pump through leads.
By the scheme, the invention at least has the following advantages: the automatic insoluble recombinant protein recovery device realizes direct measurement of the turbidity of the solution through the arrangement of the photosensitive device, and realizes automatic control of the flow rate of the water pump (for example, the flow rate is reduced from 1 ml/min to 0.5 ml/min) through the feedback of the controller, thereby controlling the real-time concentration of the denaturant in the dialysate. During specific work, an operator inserts a protein dialysis tube with insoluble recombinant protein inside into the dialysis tank through an input port at the top of the dialysis tank and fixes the protein dialysis tube, and the bottom end of the protein dialysis tube is positioned in urea solution in the dialysis tank. Because the concentration of the urea in the external dialysate is lower than the concentration in the protein dialysis tube, the urea is gradually dialyzed into the dialysis tank from the protein dialysis tube through the dialysis membrane, so that the concentration of the urea in the protein dialysis tube is reduced, and the recombinant protein in the protein dialysis tube is gradually recovered. In the process of resuscitation, due to the reduction of the urea concentration, partial protein precipitates again, the turbidity in the protein dialysis tube is gradually increased, light emitted by the light source passes through the protein dialysis tube and then is scattered, the light (Iout) at the output end is smaller than that at the input end (Iin), and the difference size and the change speed reflect the condition of protein precipitation. The photosensitive device converts the optical signal into an electric signal and transmits the electric signal to the controller, and the controller determines whether to change the change speed of the concentration of the denaturant or not so as to reduce protein precipitation after receiving and judging the information. The whole process is shown in figure 3 in the specification.
In conclusion, the automatic insoluble recombinant protein recovery device can feed back and automatically control the water flow and the water flow speed according to the turbidity of the recombinant protein solution so as to promote revival to the maximum extent and reduce precipitation.
The foregoing description is only an overview of the technical solutions of the present invention, and in order to make the technical solutions of the present invention more clearly understood and to implement them in accordance with the contents of the description, the following detailed description is given with reference to the preferred embodiments of the present invention and the accompanying drawings.
Drawings
FIG. 1 is a schematic diagram of the structure of an automated insoluble recombinant protein resuscitation device of the present invention;
FIG. 2 is a schematic view of a partial structure of a dialysis tank and a protein dialysis tube;
FIG. 3 is a schematic flow diagram of the operation of the automated insoluble recombinant protein resuscitation apparatus of the present invention.
In the figure, 1: a dialysis tank; 2: a protein dialysis tube; 3: a dialysis membrane; 4: a light source; 5: a photosensitive device; 6: a water pump; 7: a controller; 8: a positioning flange; 9: a magnetic stirrer; 10: a blender; 11: a water storage tank; 12: a waste water tank; 13: and (4) an upper computer.
Detailed Description
The following detailed description of embodiments of the present invention is provided in connection with the accompanying drawings and examples. The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.
Referring to fig. 1 to 3, an automated insoluble recombinant protein resuscitation device according to a preferred embodiment of the present invention includes a dialysis tank 1, a protein dialysis tube 2 made of a transparent material is disposed on a top of the dialysis tank, a bottom end of the protein dialysis tube passes through a top wall of the dialysis tank and extends into an inner cavity of the dialysis tank, a dialysis membrane 3 is disposed on the protein dialysis tube disposed in the inner cavity of the dialysis tank, an inner side and an outer side of the dialysis membrane are respectively communicated with the inner cavity of the protein dialysis tube and the inner cavity of the dialysis tank, a light source 4 and a photosensitive device 5 are disposed on an outer side of the protein dialysis tube disposed above the top wall of the dialysis tank, the automated insoluble recombinant protein resuscitation device further includes a water pump 6 and a controller 7, a water outlet end of the water pump is communicated with the dialysis tank through a pipeline, and the controller is connected with an electrical input end of the water pump and an output end of the photosensitive device through.
The light I-in emitted by the light source passes through the protein dialysis tube and then reaches the photosensitive device I-out. The light-sensitive device converts the light signal into an electric signal and transmits the electric signal to the controller after receiving the light emitted by the light source, and the controller calculates the light absorbance O.D. through the electric signal after receiving the electric signal, so as to indirectly judge the turbidity of the solution in the protein dialysis tube and the change speed of the turbidity, and judge whether the protein restarts to precipitate and the precipitation speed according to the turbidity of the solution. On the basis, the controller can adjust the water inlet speed of the automatic control water pump according to a preset 'acceptable' or 'unacceptable' parameter, so that the change speed of the concentration of the denaturant in the dialysis tank is changed to reduce precipitation to the maximum extent and promote resuscitation.
During specific work, an operator inserts a protein dialysis tube with insoluble recombinant protein inside into the dialysis tank through an input port at the top of the dialysis tank, and the bottom end of the protein dialysis tube is positioned in urea solution in the dialysis tank. After that, the urea in the protein dialysis tube is gradually dialyzed into the dialysis tank through the dialysis membrane, and the urea concentration in the protein dialysis tube is gradually reduced, for example, from 4M to 2M, along with the gradual dialysis of the urea, so that the recombinant protein in the protein dialysis tube is gradually revived. The light emitted by the light source is transmitted to the input end of the photosensitive device through the transmission of the solution in the protein dialysis tube, the photosensitive device converts the light signal into an electric signal after receiving the light signal and transmits the electric signal to the controller, the controller judges the change of the turbidity of the solution according to the difference between the incident light I-in and the output light I-out, if the change of the turbidity rate is excessively accelerated, the controller sends a control signal to the water pump to reduce the speed of pumping the water, so that the speed of the concentration of the denaturant in the dialysate is reduced, the speed of re-precipitation in the protein dialysis tube is reduced, and the recovery of the recombinant protein is promoted.
Preferably, the outer side wall of the protein dialysis tube is provided with a positioning flange 8.
The positioning flange is arranged to realize the positioning of the protein dialysis tube. Specifically, operating personnel stretches into in the dialysis jar with the bottom of albumen dialysis pipe, the roof contact of locating flange and dialysis jar to the realization is to the location and the supporting role of albumen dialysis pipe.
Preferably, the photosensitive device and the light source are respectively positioned at the left side and the right side of the protein dialysis tube.
When the light source works, light emitted by the light source enters the protein dialysis tube through the transparent protein dialysis tube and is transmitted to the corresponding photosensitive device through solution in the protein dialysis tube and the side wall of the other side of the protein dialysis tube.
Preferably, a magnetic stirrer 9 is arranged below the dialysis tank.
The arrangement of the magnetic stirrer realizes the stirring operation of the urea solution in the dialysis tank, thereby enabling the urea solution to be more uniform.
Preferably, a stirrer 10 is arranged on the top wall of the protein dialysis tube, the body of the stirrer is positioned on the top wall of the protein dialysis tube, and the output shaft of the stirrer penetrates through the protein dialysis tube and extends into the protein dialysis tube.
The setting of mixer has realized the stirring operation to solution in the albumen dialysis tube to make its turbidity more even, measure more accurately.
Preferably, the water-saving device also comprises a water storage tank 11, and the water inlet end of the water pump is communicated with the inner cavity of the water storage tank through a pipeline.
Preferably, the device also comprises a waste water tank 12, and the inner cavity of the waste water tank is communicated with the inner cavity of the dialysis tank through a pipeline.
Preferably, the light source is a visible light, the photosensitive device is a photosensitive resistor, the water pump is a stepping pump, the controller comprises a substrate and a single chip microcomputer arranged on the substrate, and a port of the single chip microcomputer is connected with the photosensitive resistor and an electric input end of the stepping pump through leads.
Specifically, the single chip microcomputer adopts an STC12C5A60S2 single chip microcomputer, one end of a photoresistor is grounded, the other end of the photoresistor is connected with input voltage after being connected with a fixed resistor in series, one end of the photoresistor in series with the resistor is connected with an AD conversion port of the single chip microcomputer through a lead, a general input/output port of the single chip microcomputer is connected with an input end of the ULN2803 through a lead, and an output end of the ULN2803 is connected with a pulse signal input end of a stepping pump, so that the collection of voltage signals of the photoresistor and. When the device works specifically, the resistance value of the photosensitive resistor is reduced after the photosensitive resistor receives an optical signal, a voltage signal input by an AD conversion port of the single chip microcomputer changes, the single chip microcomputer converts voltage data into a 10-bit digital signal, and a program stored in the single chip microcomputer judges the resistance value of the photosensitive resistor and the intensity of light according to the digital signal so as to judge the turbidity in the protein dialysis tube. Or the singlechip transmits the digital signal to the upper computer 13 through a corresponding interface, and the digital signal is processed by the upper computer and returns a corresponding processing result. And then the singlechip controls the pulse frequency through the input and output ports according to the turbidity signal, and further drives a stepping motor of the stepping pump to rotate through the ULN2803, so that the control of the water quantity and the flow rate is realized.
In addition, regarding the measurement principle of the light source and the photosensitive device, reference may also be made to the article "structure, working principle and application of ultraviolet-visible spectrophotometer" in the hundred-degree library, and the concrete websites thereof are: https:// wenku.baidu.com/view/373c064e6c85ec 3a87c2c554.html.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description.
In addition, the above is only a preferred embodiment of the present invention, and is not intended to limit the present invention, it should be noted that, for those skilled in the art, several modifications and variations can be made without departing from the technical principle of the present invention, and these modifications and variations should also be regarded as the protection scope of the present invention. Also, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (8)
1. The utility model provides a full-automatic insoluble recombinant protein resuscitates device, includes the dialysis jar, its characterized in that: the top of dialysis jar is provided with the albumen dialysis tube of making by transparent material, and the bottom of albumen dialysis tube is passed the roof of dialysis jar stretches into to the inner chamber of dialysis jar, is located to be equipped with the dialysis membrane on the albumen dialysis tube in the dialysis jar inner chamber, the inside and outside both sides of dialysis membrane respectively with the inner chamber of albumen dialysis tube, the inner chamber intercommunication of dialysis jar, the outside of the albumen dialysis tube that is located dialysis jar top wall top is equipped with light source and photosensitive device, automatic insoluble recombinant protein resuscitator still includes water pump and controller, the play water end of water pump pass through the pipeline with dialysis jar intercommunication, the controller through wired or wireless connection the mode with the electric input end of water pump, photosensitive device's output are connected.
2. The automated insoluble recombinant protein resuscitation device of claim 1, wherein: and a positioning flange is arranged on the outer side wall of the protein dialysis tube.
3. The automated insoluble recombinant protein resuscitation device of claim 1, wherein: the photosensitive device and the light source are respectively positioned at the left side and the right side of the protein dialysis tube.
4. The automated insoluble recombinant protein resuscitation device of claim 1, wherein: a magnetic stirrer is arranged below the protein dialysis tube.
5. The automated insoluble recombinant protein resuscitation device of claim 1, wherein: the top wall of the protein dialysis tube is provided with a stirrer, the body of the stirrer is positioned on the top wall of the protein dialysis tube, and the output shaft of the stirrer penetrates through the protein dialysis tube and extends into the protein dialysis tube.
6. The automated insoluble recombinant protein resuscitation device of claim 1, wherein: the water pump is characterized by further comprising a water storage tank, and the water inlet end of the water pump is communicated with the inner cavity of the water storage tank through a pipeline.
7. The automated insoluble recombinant protein resuscitation device of claim 1, wherein: still include the waste water jar, the inner chamber of waste water jar pass through the pipeline with the inner chamber intercommunication of dialysis jar.
8. The automated insoluble recombinant protein resuscitation device of claim 1, wherein: the light source is an ultraviolet lamp, the photosensitive device is a photosensitive resistor, the water pump is a stepping pump, the controller comprises a substrate and a single chip microcomputer arranged on the substrate, and a port of the single chip microcomputer is connected with electrical input ends of the photosensitive resistor and the stepping pump through a lead.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811098071.4A CN110922449A (en) | 2018-09-20 | 2018-09-20 | Automatic insoluble recombinant protein resuscitation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811098071.4A CN110922449A (en) | 2018-09-20 | 2018-09-20 | Automatic insoluble recombinant protein resuscitation device |
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| Publication Number | Publication Date |
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| CN110922449A true CN110922449A (en) | 2020-03-27 |
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| CN201811098071.4A Withdrawn CN110922449A (en) | 2018-09-20 | 2018-09-20 | Automatic insoluble recombinant protein resuscitation device |
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|---|---|---|---|---|
| CN1473843A (en) * | 2003-07-24 | 2004-02-11 | 华东理工大学 | Industrially extracting, renaturation and purifying method for recombined TRAILinclusion body protein |
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| CN102169079A (en) * | 2010-12-24 | 2011-08-31 | 宁波水表股份有限公司 | Device for detecting turbidity on line and in real time |
| CN102653551A (en) * | 2011-03-02 | 2012-09-05 | 复旦大学 | Denatured protein renaturation device |
| CN103665097A (en) * | 2012-09-06 | 2014-03-26 | 复旦大学 | Denatured protein renaturation device and renaturation method |
| CN204185410U (en) * | 2014-09-28 | 2015-03-04 | 南京钟鼎生物技术有限公司 | A kind of efficient, automatic metaprotein dialysis renaturation device |
| CN104884606A (en) * | 2012-11-30 | 2015-09-02 | 株式会社百奥尼 | Apparatus for automatically preparing cell-free proteins and method for preparing proteins using same |
| CN106699836A (en) * | 2017-03-07 | 2017-05-24 | 安徽鑫华坤生物工程有限公司 | Dialysis equipment for protein renaturation |
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2018
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Patent Citations (8)
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|---|---|---|---|---|
| CN1473843A (en) * | 2003-07-24 | 2004-02-11 | 华东理工大学 | Industrially extracting, renaturation and purifying method for recombined TRAILinclusion body protein |
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| CN102169079A (en) * | 2010-12-24 | 2011-08-31 | 宁波水表股份有限公司 | Device for detecting turbidity on line and in real time |
| CN102653551A (en) * | 2011-03-02 | 2012-09-05 | 复旦大学 | Denatured protein renaturation device |
| CN103665097A (en) * | 2012-09-06 | 2014-03-26 | 复旦大学 | Denatured protein renaturation device and renaturation method |
| CN104884606A (en) * | 2012-11-30 | 2015-09-02 | 株式会社百奥尼 | Apparatus for automatically preparing cell-free proteins and method for preparing proteins using same |
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Application publication date: 20200327 |