CN205152131U - Portable nickel post auxiliary device - Google Patents

Portable nickel post auxiliary device Download PDF

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Publication number
CN205152131U
CN205152131U CN201520849176.4U CN201520849176U CN205152131U CN 205152131 U CN205152131 U CN 205152131U CN 201520849176 U CN201520849176 U CN 201520849176U CN 205152131 U CN205152131 U CN 205152131U
Authority
CN
China
Prior art keywords
nickel post
portable
utility
incubation chamber
model
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201520849176.4U
Other languages
Chinese (zh)
Inventor
王安桥
肖运祥
肖银川
宋现收
曹启龙
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
HUBEI YINFENG DINGCHENG BIOLOGY ENGINEERING Co Ltd
Original Assignee
HUBEI YINFENG DINGCHENG BIOLOGY ENGINEERING Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by HUBEI YINFENG DINGCHENG BIOLOGY ENGINEERING Co Ltd filed Critical HUBEI YINFENG DINGCHENG BIOLOGY ENGINEERING Co Ltd
Priority to CN201520849176.4U priority Critical patent/CN205152131U/en
Application granted granted Critical
Publication of CN205152131U publication Critical patent/CN205152131U/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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Abstract

The utility model belongs to the technical field of the protein purification and specifically relates to a portable nickel post auxiliary device. It includes iron stand platform, receipt base, box and fixed clamping device keep warm, receive the pedestal mounting in the bottom that keeps warm the box, the box that keeps warm is installed on the iron plate of iron stand platform, fixed clamping device installs on the iron prop of iron stand platform, drainage pipe is installed to the box that keeps warm, the lateral wall with dissection of heat preservation box, pack the coolant liquid in the intermediate layer. The utility model discloses the nickel post is fixed convenient simple, realizes that low temperature environment receives the egg gathering white matter, and the stability of guaranteeing protein is with active, and simplified operation avoids polluting, improves protein purification quality and success rate. The utility model discloses the inconvenience that manually operation brought has been avoidd in the simply easy operation of instrument, has reduced the uneven first -class error of artificial protein purification. The utility model discloses portable nickel post purification container is small, can put and operate in biohazard safety equipment, and low temperature environment is stabilized in convenient operation.

Description

A kind of portable nickel post supplementary unit
Technical field
The utility model relates to protein purification field, specifically a kind of portable nickel post supplementary unit.
Background technology
The separation and purification of protein uses extensively in biochemical research application, is an important operative technique.A typical eukaryotic cell can comprise thousands of different proteins, and some content are very abundant, and some are only containing several copy.In order to study some protein, must first this protein be purified from other protein and non-proteinaceous molecule.
Laboratory generally adopts nickel post to carry out purifying.But the protein mass of ni-sepharose purification is little, and the small volume of nickel post, not easy-to-use iron stand is fixed, and is inconvenient to again move once fixing.When collecting protein, because protein needs temperature and the activity of low temperature environment guarantee protein, complex operation.Another, is because protein collection tube is directly placed on ice face, easy contaminating protein matter.
At present, the purifying of nickel post is generally by a Foam Container, the ice cube of certain volume is filled in the inside, collection tube is directly placed on ice face, nickel post is directly placed on the mouth of pipe of collection tube, collect a pipe manually to move on to afterwards on another collection tube, cause fixing instability because collection tube is directly placed on ice face.Moreover nickel post is placed on collection tube often can topple over the protein losses causing collecting by generation collection tube, and be also easy to pollute, nickel post is placed on the collection mouth of pipe, cannot fix, and frequent nickel post surface tilts, and causes the albumen heterogeneity of collection simultaneously.
Summary of the invention
Technical assignment of the present utility model is for the deficiencies in the prior art, a kind of portable nickel post supplementary unit is provided, stable low temperature environment can be provided, ensure that the stability of protein is with active, simplify manual operation, instrument is simple to operation, improves repeatability and the success ratio of target protein matter purifying.
The utility model solves the technical scheme that its technical problem adopts:
A kind of portable nickel post supplementary unit, comprise iron stand, receive base, incubation chamber and fixed clamp device, described reception floor installation is in the bottom of incubation chamber, described incubation chamber is arranged on the iron plate of iron stand, described fixed clamp device is arranged on the iron prop of iron stand, described incubation chamber is provided with drainage pipeline, and the sidewall of described incubation chamber is provided with interlayer, fills cooling fluid in described interlayer.
Further, described fixed clamp device is provided with sliding rail, and nickel post is arranged on sliding rail.
Further, described sliding rail comprises cross track and vertical track, and nickel post is arranged on cross track and vertical node of orbit place, and the motion of described cross track and vertical track has driven the motion of nickel post.
Further, described cross track and vertical track side are equipped with handle.
Further, described cooling fluid can replace with ice cube.
Further, described reception base card and the bottom in incubation chamber, detachably.
Further, described reception base is at least one.
Further, described incubation chamber is stainless steel.
The utility model nickel post convenient fixing is simple, realizes low temperature environment and collects protein, ensures that the stability of protein is with active, simplifies the operation, avoids polluting, improve protein purification quality and success ratio.The utility model instrument is simple to operation, has evaded the inconvenience that manual operation brings, and decreases artificial protein purification heterogeneity equal error.The utility model portable ni-sepharose purification container volume is little, can be placed in Biohazard Safety Equipment and operate, handled easily, stablize low temperature environment, greatly reduce pollution rate, reduces nickel post surface and tilts to cause target protein heterogeneity equal error.
Accompanying drawing explanation
Below in conjunction with drawings and Examples, the utility model is described in further detail.
Fig. 1 is the utility model one-piece construction schematic diagram;
Fig. 2 is the utility model incubation chamber 3 sectional view;
Fig. 3 is the utility model fixed clamp device 4 schematic diagram;
Embodiment
In order to make the purpose of this utility model, technical scheme and advantage clearly understand, below in conjunction with specific embodiment, the utility model is further elaborated.
As shown in Figure 1, 2, 3, a kind of portable nickel post supplementary unit, comprise iron stand 1, receive base 2, incubation chamber 3 and fixed clamp device 4, described reception base 2 is arranged on the bottom of incubation chamber 3, described reception base 2 blocks and in the bottom of incubation chamber 3, detachably, the timely replacing of personnel easy to use.Described incubation chamber 3 is arranged on the iron plate of iron stand 1, the sidewall of described incubation chamber 3 is provided with interlayer 31, fills cooling fluid in described interlayer 31, keeps the temperature in incubation chamber 3 in time, described incubation chamber 3 is provided with drainage pipeline 32, facilitates discharge and the replacing of liquid in incubation chamber 3.
Described fixed clamp device 4 is arranged on the iron prop of iron stand 1, described fixed clamp device 4 is provided with sliding rail 41, described sliding rail 41 comprises cross track and vertical track, nickel post 5 is arranged on cross track and vertical node of orbit place, and the motion of described cross track and vertical track has driven the motion of nickel post 5.Described cross track and vertical track side are equipped with handle 42, and person easy to use is controlled by handle 42 pairs of cross tracks and vertical track, thus drive the motion of nickel post 5, facilitate the protein in nickel post 5 to flow to and receive in base 2, collect protein in time.Described incubation chamber 3 is stainless steel, durable.
The utility model nickel post convenient fixing is simple, realizes low temperature environment and collects protein, ensures that the stability of protein is with active, simplifies the operation, avoids polluting, improve protein purification quality and success ratio.The utility model instrument is simple to operation, has evaded the inconvenience that manual operation brings, and decreases artificial protein purification heterogeneity equal error.The utility model portable ni-sepharose purification container volume is little, can be placed in Biohazard Safety Equipment and operate, handled easily, and stable low temperature environment, greatly reduces pollution, reduces nickel post surface and tilts to cause target protein heterogeneity equal error.
Although be described preferred embodiment of the present utility model above; but the utility model is not limited to above-mentioned embodiment; above-mentioned embodiment is only schematic; be not restrictive; those of ordinary skill in the art is under enlightenment of the present utility model; do not departing under the ambit that the utility model aim and claim protect, can also make a lot of form, these all belong within protection domain of the present utility model.

Claims (8)

1. a portable nickel post supplementary unit, comprise iron stand, it is characterized in that: also comprise and receive base, incubation chamber and fixed clamp device, described reception floor installation is in the bottom of incubation chamber, described incubation chamber is arranged on the iron plate of iron stand, and described fixed clamp device is arranged on the iron prop of iron stand, and described incubation chamber is provided with drainage pipeline, the sidewall of described incubation chamber is provided with interlayer, fills cooling fluid in described interlayer.
2. one according to claim 1 portable nickel post supplementary unit, is characterized in that: described fixed clamp device is provided with sliding rail, and nickel post is arranged on sliding rail.
3. one according to claim 2 portable nickel post supplementary unit, it is characterized in that: described sliding rail comprises cross track and vertical track, nickel post is arranged on cross track and vertical node of orbit place, and the motion of described cross track and vertical track has driven the motion of nickel post.
4. one according to claim 3 portable nickel post supplementary unit, is characterized in that: cross track and vertical track side are equipped with handle.
5. one according to claim 1 portable nickel post supplementary unit, is characterized in that: described cooling fluid can replace with ice cube.
6. one according to claim 1 portable nickel post supplementary unit, is characterized in that: described reception base card and the bottom in incubation chamber, detachably.
7. the portable nickel post of the one according to claim 1 or 6 supplementary unit, is characterized in that: described reception base is at least one.
8. one according to claim 1 portable nickel post supplementary unit, is characterized in that: described incubation chamber is stainless steel.
CN201520849176.4U 2015-10-24 2015-10-24 Portable nickel post auxiliary device Expired - Fee Related CN205152131U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201520849176.4U CN205152131U (en) 2015-10-24 2015-10-24 Portable nickel post auxiliary device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201520849176.4U CN205152131U (en) 2015-10-24 2015-10-24 Portable nickel post auxiliary device

Publications (1)

Publication Number Publication Date
CN205152131U true CN205152131U (en) 2016-04-13

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN201520849176.4U Expired - Fee Related CN205152131U (en) 2015-10-24 2015-10-24 Portable nickel post auxiliary device

Country Status (1)

Country Link
CN (1) CN205152131U (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106699836A (en) * 2017-03-07 2017-05-24 安徽鑫华坤生物工程有限公司 Dialysis equipment for protein renaturation

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106699836A (en) * 2017-03-07 2017-05-24 安徽鑫华坤生物工程有限公司 Dialysis equipment for protein renaturation

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C14 Grant of patent or utility model
GR01 Patent grant
C56 Change in the name or address of the patentee
CP02 Change in the address of a patent holder

Address after: 443000 Hubei Province, Yichang city Wujiagang District in the South Xingfa square building 15A-301

Patentee after: HUBEI YINFENG DINGCHENG BIOLOGY ENGINEERING CO., LTD.

Address before: 443000 Hubei Province, Yichang city Wujiagang District Yiling Road No. 183 fifteenth storey clinic

Patentee before: HUBEI YINFENG DINGCHENG BIOLOGY ENGINEERING CO., LTD.

CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20160413

Termination date: 20181024

CF01 Termination of patent right due to non-payment of annual fee