CN206705999U - A kind of dialysis machine of protein renaturation - Google Patents
A kind of dialysis machine of protein renaturation Download PDFInfo
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- CN206705999U CN206705999U CN201720219477.8U CN201720219477U CN206705999U CN 206705999 U CN206705999 U CN 206705999U CN 201720219477 U CN201720219477 U CN 201720219477U CN 206705999 U CN206705999 U CN 206705999U
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Abstract
The utility model belongs to bio-pharmaceuticals apparatus field, more particularly to a kind of dialysis machine of protein renaturation, including accommodates the container of renaturation solution, and described external container is attached to cooling heat-insulation layer.The utility model sets up cooling heat-insulation layer in external container, constantly the renaturation solution in container can be cooled down using heat-insulation layer is cooled down, so as to ensure dialysis renaturation temperature, and then ensure dialysis renaturation effect, dialysis renaturation operation can also be simplified simultaneously, improve dialysis renaturation efficiency.
Description
Technical field
The utility model belongs to bio-pharmaceuticals apparatus field, more particularly to a kind of dialysis machine of protein renaturation.
Background technology
Escherichia coli generally express exogenous proteins with inclusion bodies, and the protein in inclusion body is in denatured state,
Need to carry out renaturation process to it that bioactivity could be obtained.The refolding method commonly used at present in production is dialysis renaturation, with height
Concentration guanidine hydrochloride dissolution inclusion body, it is sealed against in bag filter, being subsequently placed in large volume renaturation solution or dialyzate, using saturating
The semi-transparent film character of bag is analysed, guanidine hydrochloride progressively can be discharged into renaturation solution from bag filter, and protein is able to renaturation in the process.
When carrying out dialysis renaturation operation, renaturation solution needs to be placed in precooling in refrigerator or low temperature workshop after configuring, and takes time and effort, and dialyses
Efficiency is low, and dialyse when renaturation solution temperature it is uncontrollable, cause dialysis degree uncontrollable.
Utility model content
The purpose of this utility model is that providing one kind is not required to carry out precooling to renaturation solution, can effectively control dialysis renaturation temperature
The dialysis machine of the protein renaturation of degree.
To realize object above, the technical solution adopted in the utility model is：A kind of dialysis machine of protein renaturation, bag
The container for accommodating renaturation solution is included, described external container is attached to cooling heat-insulation layer.
Compared with prior art, there is following technique effect in the utility model：The utility model is set up cold in external container
But heat-insulation layer, constantly the renaturation solution in container can be cooled down using heat-insulation layer is cooled down, so as to ensure that dialysis is multiple
Warm-natured degree, and then ensure dialysis renaturation effect, while dialysis renaturation operation can also be simplified, improve dialysis renaturation efficiency.
Brief description of the drawings
The content expressed by each width accompanying drawing of this specification and the mark in figure are briefly described below：
Fig. 1 is structural representation of the present utility model；
Fig. 2 is the structural representation of the utility model hanger and Dialysis container.
In figure：10. container, 11. cavity volumes, 111. inlets, 112. liquid outlets, 12. liquid pumps, 121. entrances, 122. outlets,
13. hanger, 14. monitoring alarms, 20. outside plates, 21. clip cavities, 30. heat-insulation layers, 40. Dialysis containers.
Embodiment
Below in conjunction with the accompanying drawings 1,2, by the description to embodiment, specific embodiment of the present utility model is made further
Describe in detail.
A kind of dialysis machine of protein renaturation, including the container 10 of renaturation solution is accommodated, set up outside described container 10
There is cooling heat-insulation layer 30.Cooling heat-insulation layer 30 can be cooled down constantly to the renaturation solution in container 10, so as to ensure
Dialysis renaturation temperature, and then ensure dialysis renaturation effect, while dialysis renaturation operation is also simplify, improve dialysis renaturation effect
Rate.The equipment can be used for the dialysis renaturation of the protein such as IFN, KGF, EGF, aFGF, can be used for the renaturation of other protein.
The various structures of heat-insulation layer 30 are cooled down, here preferably, described cooling heat-insulation layer 30 includes being arranged on container 10
Outside outside plate 20, the clip cavity 21 between outside plate 20 and container 10 are connected with low-temperature receiver, enable low-temperature receiver clip cavity 21 is interior and container
Renaturation solution in 10 carries out fully heat exchange, ensures cooling effect.Low-temperature receiver should select its type and cooling according to renaturation requirement
Temperature, to adapt to the dialysis of the renaturation of different proteins.In the present embodiment preferably, pipe is passed through using flow-like low-temperature receiver, clip cavity 21
Road connects circulating pump and forms circulation loop therewith, and circulating pump freezing low-temperature receiver simultaneously carries for its circulating in circulation loop
For power, to ensure stable cooling effect.Further, heat-insulation layer 30 is attached to outside described outside plate 20, avoids low-temperature receiver
Consumption, improve cooling heat-insulation layer 30 cooling effectiveness.
Preferably, the container 10 is provided with the inlet 111 and liquid outlet 112 for changing liquid online, is easy to renaturation solution more
Change, it is easy to operate, quick, annealing efficiency can be effectively improved.For stroke of the increase renaturation solution in cavity volume 11, renaturation solution is lifted
Utilization ratio, inlet 111 are located at the first half of container 10, and liquid outlet 112 is located at the cavity bottom of container 10.
Similarly, in order to accelerate dialysis renaturation speed, here preferably, in addition to renaturation solution is driven in the cavity volume of container 10
Convection current or circulated in 11, make the mixed uniformly liquid pump 12 of renaturation liquid energy, entrance 121 and the outlet 122 of liquid pump 12 are submerged multiple
Property liquid in and away from being arranged in cavity volume 11.Renaturation solution is pumped into liquid pump 12 from entrance 121 and pumped out from outlet 122, ensures multiple
Property liquid fully contacted with Dialysis container 40 in the cavity volume 11, and the uniformity of renaturation solution concentration can be improved.The number of liquid pump 12
Amount and its entrance 121, the arrangement of outlet 122 should be adjusted according to the volume of container 10, to ensure the uniform mixing of renaturation solution.
Liquid pump 12 can select peristaltic pump or immersible pump, during from peristaltic pump, the pump line of renaturation solution Scrape pump, not contact pump
Body, the cleaning of renaturation solution can be effectively ensured；During from immersible pump, the immersible pump pump housing is arranged in container 10 using sucker
On wall, install convenient, it is easy to the adjustment of its quantity and installation site.
Preferably, in order to prevent the Dialysis container 40 for accommodating denaturing liquid from being collided in whipping process, Dialysis container is made
40 seal failure, causes the loss that denaturing liquid is unnecessary, and the liquid level of renaturation solution is provided with hanger 13 in described container 10, hangs
The underhung of frame 13 has the Dialysis container 40 for accommodating denaturing liquid.In the present embodiment preferably, hanger 13 rides upon the top of container 10,
Dialysis container 40 is suspended on the lower section of hanger 13 with soft rope, had both enable the free movement within the specific limits of Dialysis container 40, and had prevented again
More violent collision, which occurs, for Dialysis container 40 causes to rupture, and dialytic efficiency is effectively ensured.
Preferably, monitoring alarm 14 being provided with described container 10, monitoring alarm 14 includes measurement sensor,
Measurement sensor is electrically connected with communication module.Measurement sensor can select pH measurement sensors, conductivity measurement sensors and
Temperature sensor etc., when the measurement data such as pH, electrical conductivity, temperature exceed predetermined scope, reception of the communication module to setting
Equipment carries out communication alert, so as to ensure dialysis renaturation effect.
Preferably, described cooling heat-insulation layer 30 bottom is provided with the roller for being easy to equipment to move, to realize the facility of equipment
Using.
Claims (7)
1. a kind of dialysis machine of protein renaturation, including accommodate the container (10) of renaturation solution, it is characterised in that：Described container
(10) outside to be attached to cooling heat-insulation layer, described cooling heat-insulation layer includes being arranged on the outside outside plate (20) of container (10), outside
Clip cavity (21) between plate (20) and container (10) is connected with low-temperature receiver.
2. equipment according to claim 1, it is characterised in that：Heat-insulation layer (30) is attached to outside described outside plate (20).
3. equipment according to claim 1, it is characterised in that：The container (10) is provided with the inlet for changing liquid online
(111) and liquid outlet (112), inlet (111) are located at the first half of container (10), and liquid outlet is located at the chamber bottom of container (10)
Portion.
4. the equipment according to claim 1 or 3, it is characterised in that：Also include driving renaturation solution in the cavity volume of container (10)
(11) liquid pump (12) of internal circulation flow, entrance (121) and the outlet (122) of liquid pump (12) are submerged in renaturation solution and away from arrangement
In cavity volume (11).
5. equipment according to claim 1, it is characterised in that：The liquid level of described container (10) interior renaturation solution, which is provided with, to be hung
Frame (13), hanger (13) underhung have the Dialysis container (40) for accommodating denaturing liquid.
6. equipment according to claim 1, it is characterised in that：Monitoring alarm is provided with described container (10)
(14), monitoring alarm (14) includes measurement sensor, and measurement sensor is electrically connected with communication module.
7. equipment according to claim 1, it is characterised in that：Described cooling heat-insulation layer bottom, which is provided with, is easy to equipment to move
Roller.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720219477.8U CN206705999U (en) | 2017-03-07 | 2017-03-07 | A kind of dialysis machine of protein renaturation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720219477.8U CN206705999U (en) | 2017-03-07 | 2017-03-07 | A kind of dialysis machine of protein renaturation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206705999U true CN206705999U (en) | 2017-12-05 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720219477.8U Active CN206705999U (en) | 2017-03-07 | 2017-03-07 | A kind of dialysis machine of protein renaturation |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106699836A (en) * | 2017-03-07 | 2017-05-24 | 安徽鑫华坤生物工程有限公司 | Dialysis equipment for protein renaturation |
-
2017
- 2017-03-07 CN CN201720219477.8U patent/CN206705999U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106699836A (en) * | 2017-03-07 | 2017-05-24 | 安徽鑫华坤生物工程有限公司 | Dialysis equipment for protein renaturation |
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