CN106692473A - Compound herbal preparation capable of relieving physical fatigue and resisting oxidation and preparation method thereof - Google Patents
Compound herbal preparation capable of relieving physical fatigue and resisting oxidation and preparation method thereof Download PDFInfo
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- CN106692473A CN106692473A CN201710012074.0A CN201710012074A CN106692473A CN 106692473 A CN106692473 A CN 106692473A CN 201710012074 A CN201710012074 A CN 201710012074A CN 106692473 A CN106692473 A CN 106692473A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/81—Solanaceae (Potato family), e.g. tobacco, nightshade, tomato, belladonna, capsicum or jimsonweed
- A61K36/815—Lycium (desert-thorn)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
- A61K36/258—Panax (ginseng)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
- A61K36/285—Aucklandia
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/29—Berberidaceae (Barberry family), e.g. barberry, cohosh or mayapple
- A61K36/296—Epimedium
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/60—Moraceae (Mulberry family), e.g. breadfruit or fig
- A61K36/605—Morus (mulberry)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/80—Scrophulariaceae (Figwort family)
- A61K36/804—Rehmannia
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
Abstract
The invention discloses a compound herbal preparation capable of relieving physical fatigue and resisting oxidation. The compound herbal preparation is prepared from, by weight, 80-200 parts of radix ginseng, 30-50 parts of Chinese wolfberry fruit, 30-60 parts of mulberry fruit, 30-50 parts of herba epimedii, 30-60 parts of prepared rehmannia roots and 10-20 parts of tissue culture of saussurea involucrate. The components are subjected to the processes of pulverizing, ultrasonic extracting, drying and the like and can be prepared into tea bags or granules or tablets or hard capsules, the effective ingredient extraction rate of the preparation is high, and the preparation is suitable for industrialized production. The preparation has the significant effects of relieving the physical fatigue, resisting oxidation and the like and is beneficial for health care, health maintenance and disease prevention if the preparation is taken for a long term.
Description
Technical field
Technical field the present invention relates to contain Chinese herbal compound preparation, and in particular to it is oxidation resistant that one kind alleviates physical fatigue
Herbal composition preparation.
Background technology
Ginseng is the root of Araliaceae Panax ginseng C.A.Mey.Ginseng Growth in 33 degree of north latitude -48 degree it
Between height above sea level, originate in Northeast China, Korea, South Korea, Japan, Russian east.Contain panaxoside 0.4%, a small amount of volatilization in root
Oil.Main Ingredients and Appearance is panacene (panacen, C15H24) 0.072% in oil.Soaping agents are isolated from root according to reports：Ginseng
Saponin A, B, C, D, E and F (panaxosideA, B, C, D, E, F) etc..Panaxoside A (C42H72O14), is panaxoside A
(ginsenosideRg1).Generation panaxatriol (panaxatriol) sapogenin after Ginsenoside B and C hydrolysis, panaxoside D,
20- tables panoxadiol (20-epiprotopanaxo-diol) sapogenin is obtained after E and F hydrolysis.Correlative study shows ginseng soap
Significantly, neutral saponin (Rb1, Rb2, Rc etc.) is without antifatigue effect, the mechanism of Anti-Fatigue Effects of Panax ginseng for the antifatigue effect of glucoside Rg1
May with its liter of high fat of blood and promote protein, RNA synthesis relevant.
Main active LBP-X is a kind of water-soluble polysaccharide in matrimony vine, and relative molecular mass 68-200 there is now
Many researchs show LBP-X have promote immune, anti-aging, it is antitumor, remove free radical, antifatigue, radioresistance, liver protection,
The effect such as reproductive function protection and improvement.
Mulberry fruit dries fruit ear for moraceae plants mulberry Morus alba L.'s, main containing sugar, tannic acid (tannic acid), apple
Tartaric acid (malicacid), vitamin (vitamin) B1.B2 and carrotene (carotene).It is mainly used in controlling in theory of traditional Chinese medical science
Treat kidney deficiency and liver and the deficiency of blood essence lose have a dizzy spell, soreness of waist tinnitus, poliosis, insomnia and dreamful sleep, injury thirst, quench one's thirst, intestinal dryness
Constipation.
Barrenwort acrid-sweet flavor is warm in nature, walks liver kidney two warp.To mend the key medicine of the gate of vitality, strengthening the essence gas, strengthening the bones and muscles, tonifying kidney and strengthening yang, face
Bed be usually used in treatment impotence in male do not lift, the disease such as involuntary emission premature ejaculation, urinary incontinence and female sterility.
Modern study shows, barrenwort containing icariine, volatile oil, ceryl alcohol, phytosterol, tannin, vitamin E etc. into
Point.The excited sexual performance of energy, has promotion gonopoiesis to act on to animal.Also it is depressured (causing peripheral vasodilation), hypoglycemic, profit
The effect of urine, kobadrin and vitamin E sample.Pharmacological experiment study shows that barrenwort can increase cardiovascular and cerebrovascular CBF, promotes
Hematopoiesis function, immunologic function and Bone m etabolism, with anti-aging, it is antitumor the effects such as.Xin Jiabo medical experts research discovery, excessive sheep
The leaves of pulse plants can effectively kill breast cancer cell, but need further research for clinic.
Saussurea involucrata culture is to prepare saussurea involucrata substitute using plant cell engineering technology, and it not only contains and natural snow lotus phase
Near active component, and also there is many pharmacological activity suitable therewith.Its main pharmacological analgesia, anti-inflammatory, wind resistance
It is wet；Suppress platelet aggregation, reducing blood lipid, improve blood circulation；There is adjustment effect to immune system；Simultaneously also anti-oxidant,
Radioresistance and many pharmacodynamic actions such as antifatigue.
The content of the invention
Goal of the invention：Deficiency the invention aims to make up existing investigative technique, there is provided a kind of safe and reliable,
Physical fatigue and oxidation resistant herbal composition preparation can be alleviated.
Technical scheme：One kind alleviate the oxidation resistant herbal composition preparation of physical fatigue, including following parts by weight each group
Part：80~200 parts of ginseng, 30~50 parts of matrimony vine, 30~60 parts of mulberry fruit, 30~50 parts of barrenwort, 30~60 parts of prepared rhizome of rehmannia, saussurea involucrata
10~20 parts of culture.
Preferably, also including auxiliary material.
Preferably, described auxiliary material is dextrin, starch.Used for compressing tablet, pelleting agent, hard shell capsules.
Preferably, described herbal composition preparation is tea bag, granule, tablet, hard shell capsules.
It is the invention also discloses the preparation method of the oxidation resistant herbal composition preparation of above-mentioned alleviation physical fatigue including as follows
Step：It is below method that bag is made tea：
1) by ginseng, Saussurea involucrata culture net system, 100 mesh sieves were crushed, dry sterilization obtains fine powder；
2) matrimony vine, barrenwort, mulberry fruit, prepared rhizome of rehmannia are mixed, crushed 10 mesh sieves, obtain mixture, add mixture 5~
Ultrasonic extraction 2 times at a temperature of 30 DEG C~60 DEG C of 7 times of ethanol of quality, 40 minutes every time, merge and extract thing liquid, extract thing liquid and subtract
Pressure is condensed into the thick paste that relative density is 1.20~1.30；
3) tea bag：By step 1) obtained by fine powder and step 2) in gained thick paste be well mixed, softwood processed, the system of waving
Grain, boiled bed drying, control moisture is obtained final product within 5% through filling, packaging.
As third object of the present invention, the method for making granule, tablet and hard shell capsules is also disclosed：Including as follows
Step：
1) by ginseng, Saussurea involucrata culture net system, 100 mesh sieves were crushed, dry sterilization obtains fine powder；
2) matrimony vine, barrenwort, mulberry fruit, prepared rhizome of rehmannia are mixed, crushed 10 mesh sieves, mixture is obtained, with mixture 5~7
Ethanol ultrasonic extraction 2 times at a temperature of 30 DEG C~60 DEG C of times quality, 40 minutes every time, merge and extract thing liquid, extract solution decompression
It is condensed into the thick paste that relative density is 1.20~1.30；
3) granule, tablet, hard shell capsules：By step 1) obtained by fine powder, step 2) in gained thick paste and appropriate amount of auxiliary materials
Well mixed, softwood processed, oscillating granulation, boiled bed drying controls moisture within 5%, through filling or compressing tablet or filling, i.e.,
.
Preferably, step 2) in ethanol mass concentration be 30%~70%.
Preferably, step 2) in, the relative density for extracting thing liquid is to be measured under the conditions of 60 DEG C.
Herbal composition preparation prepared by the present invention, it is safe and reliable, it is turned into obvious alleviation physical fatigue and antioxygen
With suitable long-term consumption.
Specific embodiment：
Embodiment 1：
In the present invention, raw material is commercially available.
By taking tea bag as an example：
One kind alleviates the oxidation resistant herbal composition preparation of physical fatigue, is made up of each component of following parts by weight：Ginseng
80 parts, 30 parts of matrimony vine, 30 parts of mulberry fruit, 30 parts of barrenwort, 30 parts of prepared rhizome of rehmannia, 10 parts of Saussurea involucrata culture.
Preparation method：
1) by ginseng, Saussurea involucrata culture net system, 100 mesh sieves were crushed, dry sterilization obtains fine powder；
2) matrimony vine, barrenwort, mulberry fruit, prepared rhizome of rehmannia are mixed, crushed 10 mesh sieves, obtain mixture, with 5 times of amounts of mixture
Mass concentration is 30% ethanol ultrasonic extraction extraction 2 times at a temperature of 40 DEG C, 40 minutes every time, merges and extracts thing liquid, is extracted
It is the thick paste of 1.25 (60 DEG C of surveys) that liquid is concentrated under reduced pressure into relative density.
3) by step 1) obtained by fine powder and step 2) in gained thick paste be well mixed, softwood processed, oscillating granulation, seethe with excitement
Bed dry, control moisture within 5%, by granule filling into 3 grams/bag tea bag, package spare.
Embodiment 2：
By taking tablet as an example：
One kind alleviates the oxidation resistant herbal composition preparation of physical fatigue, is made up of each component of following parts by weight：Ginseng
200 parts, 50 parts of matrimony vine, 60 parts of mulberry fruit, 50 parts of barrenwort, 60 parts of prepared rhizome of rehmannia, 20 parts of Saussurea involucrata culture, 15 parts of dextrin.
Preparation method：
1) by ginseng, Saussurea involucrata culture net system, 100 mesh sieves were crushed, dry sterilization obtains fine powder；
2) matrimony vine, barrenwort, mulberry fruit, prepared rhizome of rehmannia are mixed, crushed 10 mesh sieves, obtain mixture, with 7 times of amounts of mixture
Mass concentration is 70% ethanol ultrasonic extraction extraction 2 times at a temperature of 50 DEG C, 40 minutes every time, merges and extracts thing liquid, is extracted
It is the thick paste of 1.25 (60 DEG C of surveys) that thing liquid is concentrated under reduced pressure into relative density.
3) by step 1) obtained by fine powder, step 2) in gained thick paste and dextrin, be well mixed, softwood processed, the system of waving
Grain, boiled bed drying, control moisture, by granulation into 0.8 gram/piece of tablet, packages spare within 5%.
Embodiment 3：
By taking granule as an example：
One kind alleviates the oxidation resistant herbal composition preparation of physical fatigue, is made up of each component of following parts by weight：Ginseng
100 parts, 40 parts of matrimony vine, 50 parts of mulberry fruit, 40 parts of barrenwort, 50 parts of prepared rhizome of rehmannia, 15 parts of Saussurea involucrata culture, 20 parts of starch.
Preparation method：
1) by ginseng, Saussurea involucrata culture net system, 100 mesh sieves were crushed, dry sterilization obtains fine powder；
2) matrimony vine, barrenwort, mulberry fruit, prepared rhizome of rehmannia are mixed, crushed 10 mesh sieves, obtain mixture, with 6 times of amounts of mixture
Mass concentration is 70% ethanol ultrasonic extraction extraction 2 times at a temperature of 50 DEG C, 40 minutes every time, merges and extracts thing liquid, is extracted
It is the thick paste of 1.20 (60 DEG C of surveys) that thing liquid is concentrated under reduced pressure into relative density.
3) by step 1) obtained by fine powder, step 2) in gained thick paste and starch, be well mixed, softwood processed, wet method system
Grain, boiled bed drying, control moisture packages spare within 5%.
In order to further embody the beneficial effect of the application, spy provides following test example：
1. physical fatigue efficacy test is alleviated：
The gained sample tea bag particle of 1.1 Example 1.Decocted 40 minutes with by particle before experiment, decoction liquor distillation
Water is configured to by required various concentrations sample.
1.2 experimental animals：ICR male mices 40, body weight 18g~20g is tested by Disease Prevention Control Center, Hubei Prov
Animal center is provided.
1.3 key instruments and reagent
It is Dutch ISP-M types semi-automatic biochemical analyzer, SBA- bio-sensings analyzer, VIS-7200 visible spectrophotometers, even
Pulp grinder, centrifuge, electronic balance.
1.4 experimental techniques
Experimental animal is randomly divided into blank control group and three gained sample aqueous solution experimental groups of embodiment 1.Tested material
Dosage is respectively 270mg/kg.bw (low dose group), 540mg/kg.bw (middle dose group), 800mg/kg.bw (high dose group),
Blank control group animal with distilled water gavage, each test group of animals with the gained sample aqueous solution gavage of embodiment 1 of corresponding dosage,
Once a day, continuous gavage carries out following experiment respectively after 30 days.
After 1.5 swimmings with a load attached to the body experiment last gives tested material 30min, the mouse of the body weight sheet lead of root of the tail portion load 5% is put
In swimming trunk went swimming.The depth of water is no less than 30cm, 25 DEG C ± 1 DEG C of water temperature in case, record mouse freestyle swimming start to it is dead when
Between.
1.6 blood lactase acids are determined after last gives tested material 30min, and animal is put into not swimming with a load attached to the body in the water that temperature is 30 DEG C
Play 10min, and in before swimming, swimming after immediately, swimming after rest 20min respectively adopt the μ L of eyeball blood 20 determine lactic acid content.And press
Below equation calculates blood lactase acid TG-AUC, to judge blood lactase acid situation of change after moving.
Lactic acid TG-AUC=5 × (stop after 0min blood lactase acids value+2 × swimming after blood lactase acid value+3 × swimming before swimming
Breath 20min blood lactase acids value)
After 1.7 serum urea nitrogen determination lasts give tested material 30min, do not born during mouse is put into the water that temperature is 30 DEG C
Swimming 90min is revisited, serum diacetyl-oxime method measure serum urea nitrogen is taken after eyeball blood 0.5mL blood clotting is adopted after rest 60min
Content.
1.8 hepatic glycogen determine after last gives tested material 30min and put to death animal, take after liver rinses through physiological saline
In the plate being placed in after being blotted with filter paper in ice face, liver 100mg is weighed rapidly, determine hepatic glycogen content (anthrone method).
1.9 experimental datas are counted
Experimental data carries out variance analysis, heterogeneity of variance person's sum of ranks with PEMS for Windows3.0 statistical packages
Inspection statistics.
As a result：
Influence of the gained sample of embodiment 1 to Mouse Weight：It is shown in Table 1~table 4.
1~table of table 4 shows, 1 gained sample of embodiment, three original body mass of mouse between dosage group and blank control group, experiment
Body weight is without significant difference (P ＞ 0.05) at the end of mid-term and experiment.
Influence (swimming with a load attached to the body experiment) of the gained sample of 1 embodiment of table 1 to Mouse Weight
Influence (blood lactase acid measure) of the gained sample of 2 embodiment of table 1 to Mouse Weight
Influence (determination of urea nitrogen) of the gained sample of 3 embodiment of table 1 to Mouse Weight
Influence (hepatic glycogen measure) of the gained sample of 4 embodiment of table 1 to Mouse Weight
Influence of the gained sample of embodiment 1 to the mice burden swimming time：The results are shown in Table 5.
Influence of the gained sample of 5 embodiment of table 1 to the mice burden swimming time
* compared with blank control group, P ＜ 0.05
As shown in table 5, the gained sample 800mg/kg.bw dosage group mice burden swimming times of embodiment 1 be considerably longer than sky
White control group (P ＜ 0.05).
Influence of the gained sample of embodiment 1 to mouse Serum lactic acid content：It is shown in Table 6.
Influence of the gained sample of 6 embodiment of table 1 to mouse Serum lactic acid content
* compared with blank control group, P ＜ 0.05, * * compared with blank control group, P ＜ 0.01
As shown in table 6,0min Serum lactic acid contents and three time point blood breasts after 1 gained sample of embodiment, three dosage group swimming
Sour TG-AUC is substantially less than blank control group (P ＜ 0.05, P ＜ 0.01).
Lactic acid is the product of energy substance catabolism in motion process, during muscular movement, when lactic acid accumulation to certain journey
Muscular strength decline, will occur in degree, cause locomitivity to decline.Therefore, accelerate the removing of blood lactase acid, the product of sports fatigue can be slowed down
It is raw.Serum lactic acid content is substantially reduced after the experiment of each group of embodiment 1, illustrates that product of the present invention serves the work for alleviating sports fatigue
With.
Influence of the gained sample of embodiment 1 to mouse hepatic glycogen content：It is shown in Table 7.
Influence of the gained sample of 7 embodiment of table 1 to mouse hepatic glycogen content
* compared with blank control group, P ＜ 0.01
As shown in table 7, the gained sample 540mg/kg.bw dosage group hepatic glycogen contents of embodiment 1 are significantly higher than control group (P
＜ 0.01).
During physical exertion, glucose is skeletal muscle main energy sources, and the storage level of glycogen directly affects the motion of body
Ability,
When big energy is consumed in endurance exercise, hepatic glycogen is decomposed into blood first, is transported in muscle and is provided energy simultaneously
Maintain blood sugar normal level, it is to avoid hypoglycemia causes central nervous system sense of fatigue, and the content of glycogen being capable of side light fatigue
The speed or degree of generation.
Influence of the gained sample of embodiment 1 to mice serum urea nitrogen content：It is shown in Table 8.
As shown in table 8,1 gained sample of embodiment, three dosage group serum urea nitrogen contents nothing compared with blank control group is aobvious
Write difference.
Influence of the gained sample of 8 embodiment of table 1 to mice serum urea nitrogen content
Interpretation of result：This experiment uses male ICR mouse, the gained sample of embodiment 1 press human body recommended dose 10,20,
30 times of continuous gavages 30 days, as a result show 800mg/kg.bw dosage group mice burden swimming times considerably longer than blank control group,
(P ＜ 0.05).0min Serum lactic acid contents and three time point blood lactase acid TG-AUCs are substantially less than after three dosage group swimming
Blank control group (P ＜ 0.05, P ＜ 0.01).540mg/kg.bw dosage group hepatic glycogen contents are significantly higher than blank control group (P
＜ 0.01).According to experimental result, the gained sample of embodiment 1 has the effect for being relieved physical fatigue.
2. antioxidation efficacy test
It is prepared by 2.1 samples
The gained tablet 50g of Example 2, after adding water to cook 40 minutes, filtering takes filtrate, plus distilled water to amount of solution is
100ml, is to make every ml liquids equivalent to 0.5g tablets, standby；
2.2 experimental animals
Kunming kind female mice totally 50, wherein 12 monthly age above mouse 40, weight range 45-54g, 8 weeks few ages are small
Mouse 10,21.6 ± 2.6g of average weight is provided by Disease Prevention Control Center, Hubei Prov's Experimental Animal Center.
2.3 dosage are grouped
With the gained sample liquid of embodiment 2 three are set according to 133.3mg/kg.bw, 266.7mg/kg.bw, 533.3mg/kg.bw
Individual dosage group, separately sets aged mouse control group and few age mouse control group.
2.4 key instruments and reagent
751 ultraviolet specrophotometers, constant water bath box, micro adjustable sample injector, supercentrifuge and low speed centrifuge, rotation
Whirlpool vortex mixer.MDA, SOD, GSH-Px determine kit, are purchased from Nanjing and build up microbial project research institute, purchase within one week before use
Buy, 4 degree of Refrigerator stores.Chloroform used, absolute ethyl alcohol, glacial acetic acid etc. are domestic AR.
2.5 experimental techniques
To each dosage group mouse, tested material is given to animal gavage by 2% (i.e. every 100 grams of body weight fill 2ml) volume daily,
Aged controls and young control group animal give distilled water in equal volume, continuous 30 days, biological work are built up by Nanjing during off-test
MDA, SOD, GSH-Px kit specification of journey research institute purchase are determined in MDA contents, red blood cell in animal liver tissue
GSH-Px enzyme activity units in SOD vigor and whole blood.
2.6 experimental datas are counted
The variance analysis compared with control group mean using each experimental group is processed data.Software used is
PEMS3.0《Chinese medicine encyclopedia Medical Statistics》Statistical package (third edition).
Influence of the gained sample liquid of 2.7 embodiment 2 to each experimental stage body weight of mouse, the results are shown in Table 9：
Influence of the gained sample liquid of 9 embodiment of table 2 to each experimental stage body weight of mouse
From table 9：During whole experiment, aged mouse each group body weight is without significant difference (P ＞ 0.05).
The influence that the gained sample liquid of 2.8 embodiment 2 is acted on erythrocyte lipid peroxidation：The results are shown in Table 10：
Influence of the sample liquid of 10 embodiment of table 2 to MDA contents in murine liver tissue
The influence that the gained sample liquid of embodiment 2 is acted on erythrocyte lipid peroxidation, * compares with few age mouse control group aobvious
Write difference (P ＜ 0.01).# compares that there were significant differences (P ＜ 0.01) with aged mouse control group.
Table 10 shows that aged mouse control group Liver MDA is significantly higher than few age mouse control group (P ＜ 0.01), tested
The MDA contents of thing metering group high compare with aged mouse control group to be significantly reduced (P ＜ 0.01).
Influence of the gained sample liquid of 2.9 embodiment 2 to mouse red blood cell superoxide dismutase, is shown in Table 11
Influence of the sample liquid of 11 embodiment of table 2 to mouse red blood cell SOD vigor
Compare that there were significant differences (P ＜ 0.01) with few age mouse control group.#(the P that compares that there were significant differences with aged mouse control group
＜ 0.05).
Table 11 is visible, and aged mouse control group erythrocyte sod vigor is substantially less than few age mouse control group (P ＜ 0.01), tested
The SOD vigor of thing metering group high compares with aged mouse control group and dramatically increases (P ＜ 0.05).
Influence of the gained sample liquid of 2.10 embodiment 2 to Mouse whole blood glutathione peroxidase, is shown in Table 12
Influence of the sample liquid of 12 embodiment of table 2 to Mouse whole blood GSH-Px
*Compare that there were significant differences (P ＜ 0.01) with few age mouse control group.#(the P that compares that there were significant differences with aged mouse control group
＜ 0.01).
Table 12 shows that GSH-Px enzyme activity units are significantly higher than few age mouse control group (P ＜ in aged mouse control group whole blood
0.01), tested material metering group GSH-Px enzyme activity units high and aged mouse control group comparing difference are without conspicuousness (P ＞ 0.05).
The Liver MDA of high dose group is substantially less than always in the anti-oxidation function zoopery of the sample of the present embodiment 2
Age mouse control group (P ＜ 0.01), erythrocyte sod vigor is significantly higher than aged mouse control group (P ＜ 0.05), GSH-Px enzymes in whole blood
Unit of activity compares without significant difference (P ＞ 0.05) with aged mouse control group.Test result indicate that, this product has obvious antioxygen
Change is acted on.
In sum, the herbal composition preparation prepared by the present invention, safe and reliable, with it is obvious alleviate physical fatigue and
Antioxidation, long-term consumption is conducive to health care, prevention disease.
Claims (8)
- It is 1. a kind of to alleviate the oxidation resistant herbal composition preparation of physical fatigue, it is characterised in that：Each group including following parts by weight Part：80~200 parts of ginseng, 30~50 parts of matrimony vine, 30~60 parts of mulberry fruit, 30~50 parts of barrenwort, 30~60 parts of prepared rhizome of rehmannia, saussurea involucrata 10~20 parts of culture.
- It is 2. as claimed in claim 1 to alleviate the oxidation resistant herbal composition preparation of physical fatigue, it is characterised in that：Also include auxiliary Material.
- It is 3. as claimed in claim 2 to alleviate the oxidation resistant herbal composition preparation of physical fatigue, it is characterised in that：Described auxiliary material It is dextrin, starch.
- It is 4. as claimed in claim 1 to alleviate the oxidation resistant herbal composition preparation of physical fatigue, it is characterised in that：Described herbal medicine Compound preparation is tea bag, granule, tablet, hard shell capsules.
- 5. it is a kind of alleviate the oxidation resistant herbal composition preparation of physical fatigue preparation method, it is characterised in that：Comprise the following steps：1) by ginseng, Saussurea involucrata culture net system, 100 mesh sieves were crushed, dry sterilization obtains fine powder；2) matrimony vine, barrenwort, mulberry fruit, prepared rhizome of rehmannia are mixed, crushed 10 mesh sieves, mixture is obtained, with 5~7 times of matter of mixture Ultrasonic extraction 2 times at a temperature of 30 DEG C~60 DEG C of the ethanol of amount, 40 minutes every time, merge and extract thing liquid, extract thing liquid decompression dense Shorten the thick paste that relative density is 1.20~1.30 into；3) tea bag：By step 1) obtained by fine powder and step 2) in gained thick paste be well mixed, softwood processed, oscillating granulation, Boiled bed drying, control moisture is obtained final product within 5% through filling, packaging.
- 6. it is a kind of alleviate the oxidation resistant herbal composition preparation of physical fatigue preparation method, it is characterised in that：Comprise the following steps：1) by ginseng, Saussurea involucrata culture net system, 100 mesh sieves were crushed, dry sterilization obtains fine powder；2) matrimony vine, barrenwort, mulberry fruit, prepared rhizome of rehmannia are mixed, crushed 10 mesh sieves, obtain mixture, add 5~7 times of mixture Ultrasonic extraction 2 times at a temperature of 30 DEG C~60 DEG C of the ethanol of quality, 40 minutes every time, merge and extract thing liquid, and extract solution decompression is dense Shorten the thick paste that relative density is 1.20~1.30 into；3) granule, tablet, hard shell capsules：By step 1) obtained by fine powder, step 2) in gained thick paste and auxiliary material it is well mixed, Softwood processed, oscillating granulation, boiled bed drying, control moisture, through filling or compressing tablet or filling, is obtained final product within 5%.
- 7. the preparation method of a kind of alleviation oxidation resistant herbal composition preparation of physical fatigue as described in claim 5 or 6, it is special Levy and be：The mass concentration of described ethanol is 30%~70%.
- 8. the preparation method of the alleviation oxidation resistant herbal composition preparation of physical fatigue as described in claim 5 or 6, its feature exists In：Step 2) in, relative density is to be measured under the conditions of 60 DEG C.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109527561A (en) * | 2018-12-18 | 2019-03-29 | 浩仁药业(武汉)有限公司 | It is a kind of with antifatigue and strengthen immunity function Chinese medicine and health food and preparation method thereof |
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| CN102940733A (en) * | 2012-09-03 | 2013-02-27 | 吉林一正药业集团有限公司 | Composition for relieving physical fatigue and preparation method and application thereof |
| CN104560577A (en) * | 2015-02-23 | 2015-04-29 | 酒口袋（上海）投资控股有限公司 | Distilled spirit with function of resisting fatigue and preparation method of distilled spirit |
| CN104873769A (en) * | 2015-06-10 | 2015-09-02 | 陈卫兵 | Anti-aging and beautifying health care product |
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| CN102940733A (en) * | 2012-09-03 | 2013-02-27 | 吉林一正药业集团有限公司 | Composition for relieving physical fatigue and preparation method and application thereof |
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