CN106680214B - Optoacoustic laparoscopic apparatus and its method a kind of while that detect elasticity and viscosity - Google Patents
Optoacoustic laparoscopic apparatus and its method a kind of while that detect elasticity and viscosity Download PDFInfo
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/1702—Systems in which incident light is modified in accordance with the properties of the material investigated with opto-acoustic detection, e.g. for gases or analysing solids
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
- A61B5/0082—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence adapted for particular medical purposes
- A61B5/0084—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence adapted for particular medical purposes for introduction into the body, e.g. by catheters
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0093—Detecting, measuring or recording by applying one single type of energy and measuring its conversion into another type of energy
- A61B5/0095—Detecting, measuring or recording by applying one single type of energy and measuring its conversion into another type of energy by applying light and detecting acoustic waves, i.e. photoacoustic measurements
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B8/00—Diagnosis using ultrasonic, sonic or infrasonic waves
- A61B8/08—Detecting organic movements or changes, e.g. tumours, cysts, swellings
- A61B8/0833—Detecting organic movements or changes, e.g. tumours, cysts, swellings involving detecting or locating foreign bodies or organic structures
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B8/00—Diagnosis using ultrasonic, sonic or infrasonic waves
- A61B8/12—Diagnosis using ultrasonic, sonic or infrasonic waves in body cavities or body tracts, e.g. by using catheters
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B8/00—Diagnosis using ultrasonic, sonic or infrasonic waves
- A61B8/48—Diagnostic techniques
- A61B8/485—Diagnostic techniques involving measuring strain or elastic properties
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/1702—Systems in which incident light is modified in accordance with the properties of the material investigated with opto-acoustic detection, e.g. for gases or analysing solids
- G01N2021/1706—Systems in which incident light is modified in accordance with the properties of the material investigated with opto-acoustic detection, e.g. for gases or analysing solids in solids
Abstract
The invention discloses a kind of device and methods of optoacoustic laparoscope for detecting elasticity and viscosity simultaneously, and device includes laser transmitting system, optically coupled system, laparoscope system, signal transmission system and signal processing system；The laser transmitting system uses laser；The optically coupled system includes coupler, and coupler is connect by multimode fibre with laparoscope system；The laparoscope system includes collimator, condenser lens, function generator, MEMS mirror and interior snooping head, and the collimator, condenser lens, MEMS mirror are linked in sequence, and the function generator is connect with MEMS mirror, and the interior snooping head is connected to signal transmission system；The signal transmission system includes amplifier, oscillograph, lock-in amplifier.The present invention realizes X-Y plane two-dimensional scanning using MEMS mirror control laser on tissue sample, realizes that tissue sample remains stationary, and control laser carries out two-dimensional scanning；And the limitation that can overcome traditional optoacoustic surface imaging, belongs to minimally invasive detection technique field.
Description
Technical field
The present invention relates to minimally invasive detection technique field, in particular to a kind of optoacoustic laparoscope for detecting elasticity and viscosity simultaneously
Devices and methods therefor.
Background technique
Pathologic process and tissue abnormalities often with biological tissue's mechanical property, such as the close phase of change of elasticity and viscosity
It closes.Currently, widely being connect by doctor and iconography researcher by the method for the elasticity prediction tissue disease of detection tissue
It receives, such as realizes that the detection method of lossless cirrhosis has been applied to the parameter inspection of clinical disease using the method for Ultrasonic elasticity detection
It surveys.It not only can detecte using the elasticity modulus and viscosity of the accurate minimally invasive quantitative biological tissue of laparoscope, or even can also
Diagnose the lesion of a variety of diseases such as liver fibrosis.
Currently, using photoacoustic method detection tissue elasticity in China it has been reported that such as the invention disclosed on the 7th of September in 2011
Patent: photoacoustic elastic imaging method and device, applicant: South China Normal University, the applying date: on January 14th, 2011, application number:
201110008213.5, it is excited which employs the continuous light source of intensity modulated and generates ultrasonic signal, by measuring the signal and adjusting
Phase difference and point by point scanning between signal processed can reconstruct the elasticity distribution image of detection tissue sample.But in the above method
In, the quantitative measurment for realizing tissue elasticity modulus is had not been able to, that is, the absolute value of elasticity modulus cannot be obtained, phase can only be provided
To value, the accuracy of this method measurement result in practical applications is reduced.
Such as invention disclosed patent on December 16th, 2015: optoacoustic quantifies elastograph imaging method and device, applicant: south China
Normal university, the applying date: on December 16th, 2015, application number: 201510944323.0, which employs stepper motor mobile examples
Tissue realizes two-dimensional scanning, and the imaging depth of this imaging mode only has several millimeters, is only applicable to surface imaging, relevance grade is not
It is high.But this patent may be implemented tissue sample and remain stationary, and by the mobile realization two-dimensional scanning of laser optical focus, expand suitable
Use range.Laparoscope may be implemented minimally invasively to enter abdominal cavity, and operating distance is furthered as several millimeters, overcome optoacoustic surface at
The limitation of picture.
Summary of the invention
The purpose of the present invention is to solve the deficiencies of optoacoustic quantitative elasticity and viscosity imaging, provide a kind of while detecting
Elasticity and viscosity optoacoustic laparoscope device and imaging method, this method may be implemented minimally invasive, high-resolution tissue elasticity and
Sticky quantitative measurment and imaging.
In order to achieve the above object, the invention adopts the following technical scheme:
A kind of device of optoacoustic laparoscope detecting elasticity and viscosity simultaneously provided by the invention, including Laser emission system
System, optically coupled system, laparoscope system, signal transmission system and signal processing system；The laser transmitting system, optical coupling
System, laparoscope system, signal transmission acquisition system, signal processing system are linked in sequence according to the transmission direction of optical signal；Institute
Laser transmitting system is stated using laser；The optically coupled system includes coupler, and coupler passes through multimode fibre and laparoscope
System connection；The laparoscope system includes collimator, condenser lens, function generator, MEMS mirror and interior snooping head, the standard
Straight device, condenser lens, MEMS mirror are linked in sequence, and the function generator is connect with MEMS mirror, and the interior snooping head is connected to letter
Number Transmission system, the interior snooping head are ultrasonic detector；The signal transmission system includes that amplifier, oscillograph, locking phase are put
Big device；The signal processing system is computer, the ultrasonic detector, amplifier, oscillograph, lock-in amplifier and computer
It is sequentially connected, the computer is equipped with acquisition control and signal processing system；
When test, tissue sample is placed on platform, and is immersed in the coupling liquid of coupling slot；The laparoscope system is set
It sets right above tissue sample, and laparoscope, without departing from tissue sample surface, the pulse laser that the laser issues passes through light
Fine and condenser lens focuses, and impinges upon on tissue sample；The ultrasonic detector is directed at tissue sample, and the lower end of ultrasonic detector
Into in the coupling liquid of coupling slot, the photoacoustic signal that tissue sample is inspired is received；MEMS mirror in the laparoscope system
It controls pulse laser and carries out point by point scanning tissue sample.
The pulse laser wavelength that the laser issues as a preferred technical solution, is 400~2500nm, pulse width
For 1~50ns, repetition rate is 1Hz~100kHz.
The core diameter of the multimode fibre is 62.5um as a preferred technical solution,.
The MEMS mirror operating voltage 6V as a preferred technical solution, diameter 4.5mm, maximum scan range are 3cm
×3cm。
It is 0-9V, frequency range 0- that the function generator, which can produce two-way amplitude, as a preferred technical solution,
The triangular signal of 1000Hz.
The dominant frequency of the ultrasonic detector is 3MHz as a preferred technical solution, and volume is 3mm × 3mm × 5mm；Frequently
Bandwidth is 1.8-4.2MHz.
The sample rate of the oscillograph is 2.5GHz as a preferred technical solution, and the sample rate of lock-in amplifier is
102KHz, the acquisition control and signal processing system of the computer installation are formed using Labview and Matlab programming.
The present invention also provides a kind of imaging methods of optoacoustic laparoscope for detecting elasticity and viscosity simultaneously, including following steps
It is rapid:
1) tissue sample is placed on sample cell, and be immersed in the coupling liquid of coupling slot；Laparoscope is arranged in group
Right above tissue samples, and the height for adjusting laparoscope makes the focus of laparoscope without departing from tissue sample surface；By ultrasonic detector
It is directed at tissue sample, and enters the lower end of ultrasonic detector in the coupling liquid of coupling slot；
2) laser issues pulse laser, which is focused by multimode fibre, collimator and condenser lens, is formed
Focus on light beam impinges upon on MEMS mirror, and laser is impinged upon on tissue sample by MEMS mirror, inspires photoacoustic signal, and photoacoustic signal passes through
It is received after coupling liquid in coupling slot by interior snooping head；
3) snooping received photoacoustic signal is acquired, oscillography after amplifier amplifies by oscillograph and lock-in amplifier in
Device and lock-in amplifier store the signal message of acquisition into computer, function generator control MEMS mirror make pulse laser into
Row point by point scanning sample, and X-Y two-dimensional surface scanning area is formed on corresponding tissue sample；
4) after oscillograph has acquired whole signals, computer is once integrated the signal of each point to the time, obtains group
The function of time of tissue samples surface vibration displacement；Obtain tissue sample surface vibration displacement from it is above freezing rise to its maximum value when it is required
Time quantifies elasticity modulus using the tissue sample that the time calculates each point；According to the quantitative elasticity modulus of calculating, group is reconstructed
The quantitative elastic two dimensional image of tissue samples obtains optoacoustic viscoelastic image finally by the phase information that lock-in amplifier acquires, and glues
Property coefficient, which passes through, combines obtained elasticity modulus and the loss angle based on Kelvin-Voigt model to obtain.
As a preferred technical solution, in step (4), the signal of each point is carried out processing to the time by the computer is
Refer to:
Computer is once integrated the signal of each point to the time using Matlab program.
As a preferred technical solution, in step (4), the tissue sample for calculating each point quantifies elasticity modulus, uses
Following formula:
Wherein, ρ is biological tissue density, and R is laser facula radius, tmaxIt is displaced for tissue sample surface vibration from above freezing
Required time when rising to its maximum value.
The principle of the present invention is as follows:
The present invention utilizes the optical system of laparoscope and can be to the tissue of lesion plus white lumination system and CCD
It is taken pictures in real time.Optical fiber, condenser lens, MEMS mirror and optoacoustic endoscopy are popped one's head in using the instrument channel of other view laparoscope and carried out
Assembling realizes that the X-Y plane of laser scans using MEMS mirror.Interior snooping head receives photoacoustic signal, distinguishes by preamplifier
It is acquired into oscillograph and lock-in amplifier acquisition system, then by computer.After oscillograph has acquired whole signals, computer will be each
The signal of point once integrates the time, obtains the function of time of tissue sample surface vibration displacement；Obtain tissue sample table
Surface vibration displacement from it is above freezing rise to its maximum value when the time required to, quantify springform using the tissue sample that the time calculates each point
Amount；According to the quantitative elasticity modulus of calculating, the quantitative elastic two dimensional image of tissue sample is reconstructed.It is occured frequently by lock-in amplifier inspection
With the photoacoustic signal of the reference signal same frequency of Laser synchronisation output, lock-in amplifier relatively calculates measured signal and reference
The phase difference of signal is simultaneously recorded in computer.By the phase delay of computer analysis photoacoustic signal, the two-dimentional light of sample is obtained
Sound viscoelastic image.Viscosity, which passes through, combines obtained elasticity modulus and the loss angle based on Kelvin-Voigt model to obtain
It arrives.
The present invention relative to existing imaging technique have it is following the utility model has the advantages that
1, the present invention is once integrated the signal of oscillograph acquisition to the time, obtains tissue sample surface vibration displacement
The function of time, obtain tissue sample surface vibration displacement from it is above freezing rise to its maximum value when the time required to, utilize time meter
It calculates tissue and quantify elasticity modulus, compared with the method that existing relative resilient measures, not only available quantitative elasticity number, and
And there is higher accuracy as reference without normal tissue.
2, the present invention issues pulse laser using laser, and laser issues pulse laser, which passes through multimode
Optical fiber, collimator and condenser lens focus, and form focus on light beam and impinge upon on MEMS mirror, laser impinges upon tissue sample by MEMS mirror
On, photoacoustic signal is inspired, photoacoustic signal is received after the coupling liquid in coupling slot by interior snooping head.To carry out tissue bullet
Property detection not only there is higher resolution ratio compared with traditional Ultrasonic elasticity detection method, but also reduce measuring device
Volume provides possibility for clinical realization.
3, mobile example is changed to motion scan light source by the present invention, this method not only can detecte sample surfaces elasticity and
Viscosity value, and abdominal cavity can be deep into micro-wound surgical operation window, it realizes the detection in body biological tissue, is liver fibrosis,
The optoacoustic elasticity of the diseases such as cirrhosis and the clinical detection of viscosity provide possibility.
4, the quantitative elasticity of optoacoustic of the invention and the detection method of sticky laparoscope imaging have the ability quickly detected, real
The apparatus structure of existing this method is simple, easy to use, can be widely applied in the elasticity and viscosity imaging of tissue, convenient for clinic
It realizes.
Detailed description of the invention
Fig. 1 is a kind of frame principle figure of optoacoustic laparoscope for detecting elasticity and viscosity simultaneously of the embodiment of the present invention 1.
Fig. 2 is a kind of structural schematic diagram of optoacoustic laparoscope for detecting elasticity and viscosity simultaneously of the embodiment of the present invention 1.
Fig. 3 is the schematic diagram of the Agar samples of the embodiment of the present invention 2.
Fig. 4 is the optoacoustic elastic graph of the Agar samples of the embodiment of the present invention 2.
Fig. 5 is the optoacoustic viscosity figure of the Agar samples of the embodiment of the present invention 2.
Wherein, 1, laser, 2, optically coupled system, 3, laparoscope system, 3-1, multimode fibre, 3-2, collimator, 3-3,
Spatial light after collimation, 3-4, condenser lens, 3-5, function generator, 3-6, MEMS mirror, 3-7, tissue sample, 3-8, interior snooping
Head, 3-9, MEMS mirror scanning pedestal, 3-10, photoacoustic imaging window, 3-11, laparoscope outer tube wall, 3-12, lighting fiber, 3-13,
Interior snooping head signal wire, 3-14, MEMS mirror drive line, 4, signal transmission system, 5, signal processing system.
Specific embodiment
Present invention will now be described in further detail with reference to the embodiments and the accompanying drawings, but embodiments of the present invention are unlimited
In this.
Embodiment 1:
As shown in Figure 1, a kind of device of optoacoustic laparoscope for detecting elastomeric viscous simultaneously of the present embodiment includes laser hair
System 1, optically coupled system 2, laparoscope system 3, signal transmission system 4, signal processing system 5, coupling slot is penetrated (not mark in figure
Out).The laser transmitting system refers to laser 1；The optically coupled system 2 includes coupler, and coupler passes through multimode fibre
3-1 is connect with laparoscope system 3；The laparoscope system 3 include multimode fibre 3-1, collimator 3-2, condenser lens 3-4,
MEMS mirror 3-6, function generator 3-5 and interior snooping head 3-8, spatial light 3-3 of the laser after collimator 3-2 becomes collimation；
The signal transmission system includes amplifier, oscillograph, lock-in amplifier；The signal processing system refers to computer.It is described
Interior snooping head 3-8, amplifier, oscillograph, lock-in amplifier and computer are sequentially connected；The sample rate of the oscillograph is
The sample rate of 2.5GHz, lock-in amplifier are 102KHz, and the amplification factor of amplifier is 50 times；The computer is equipped with acquisition
Control and signal processing system, the system are formed using Labview and Matlab programming.
The interior snooping head 3-8 is ultrasonic detector, and dominant frequency 3MHz, volume is 3 × 3 × 5mm, bandwidth 1.8-
4.2MHz。
The imaging method for realizing a kind of optoacoustic laparoscope for detecting elastomeric viscous simultaneously of the present embodiment, mainly using following
Principle:
Periodically variable light intensity equation is provided first,
I=1/2I0[1+cos(ωt)] (1)
In above formula, I0It is time averaging light intensity, ω is modulating frequency.The damping effect generated due to the viscoplasticity of tissue
It answers, the photoacoustic waves (being equivalent to strain) that are forced to identical with excitation light frequency can generate one relative to exciting light (being equivalent to stress)
The phase delayed, in Kelvin-Voigt model, available strain-responsive,
ε (t)=εAei(ωt+δ) (2)
In above formula, εAFor the amplitude of double action strain, it is determined by dynamic strain amplitude, material constant and modulating frequency, phase
Position delay can be written as,
δ=arctan η ω/E (3)
In above formula, η is the coefficient of viscosity, and E is Young's modulus.It can be learnt by above-mentioned relation formula when one timing of modulating frequency
The relationship of phase delay and viscoelastic ratio.
After certain tissue is irradiated with a laser, the propagation of vibration will form longitudinal wave and shearing wave.It is full that laser generates shearing wave
Sufficient Navier equation, equation are as follows:
Wherein, μ is displacement of tissue, cLIt is the spread speed of longitudinal wave, cTIt is the spread speed of shearing wave, Δ is Laplce
Operator, F are radiant forces.
Equation (4) includes longitudinal wave, the shearing wave formed by a vibration source mode of propagation, it can be divided into longitudinal wave, shearing
The form of wave.In communication process, the attenuation ratio longitudinal wave of shearing wave is much bigger, thus in the communication process of shearing wave viscosity not
It can ignore.Longitudinal wave, shearing wave form are expressed as:
Wherein, uzFor along the vibration displacement of beam direction, FzAlong the radiant force of beam direction,For shearing wave
Velocity of wave, μ are modulus of shearing, and ρ is the density of substance, and ν=μ/ρ is movement shear viscosity, and η is viscosity.For Gaussian Profile
Φ=exp (- r2/R2) focusing laser beam, radiant force FzThere is following form:
Wherein r is lateral coordinates, and R is the spot radius of focal point, α is the absorption coefficient of light, I0For initial laser intensity.
φ (t) is that laser intensity changes over time relationship, fzIt is the function for describing spot size variation, is expressed asD is focal length.
According to the solution of ODE, and carries out Hankel transform and obtains:
Above equation is write as the form of Green's function:
This shear displacemant is the displacement of biological tissue surface when generating photoacoustic signal simultaneously.
Available, the μ from equation (9)zIt is the time required to reaching maximum valueIn the formula, tmaxBy organizing
Modulus of shearing, density, spot radius determine.It in other words, can be by μ=ρ (R/tmax)2Quantitative modulus of shearing.Biological tissue is several
Incompressible, Poisson's ratio is taken as 0.499, therefore by utilizing 3 μ of elasticity modulus and modulus of shearing relationship E=2 (1+ ν) μ ≈,
Wherein, ν is biological tissue's Poisson's ratio, to obtain the quantitative elasticity modulus size of tissue:
And loss angle δ and viscoelastic ratio can be learnt from (3) formulaRelationship,The viscous-elastic behaviour of tissue can be characterized.
In this way, viscosity η can be by substituting into viscoelastic ratio for quantitative elastic modulus EIt obtains.In this way, optoacoustic is fixed
Amount elasticity and viscosity imaging can be obtained by the displacement rising time and loss angle for projecting each scanning element.
Embodiment 2:
Therefore, a kind of imaging method of optoacoustic laparoscope detecting elastomeric viscous simultaneously of the present embodiment includes following step
It is rapid:
1) by Agar samples, (the rectangular agar concentration of sample background is 15g/L, body 3%；It is rectangular among sample
Agar concentration is 25g/L, and body 3% is as shown in Figure 3) sample is placed in coupling slot, and is immersed in the coupling in coupling slot
It closes in liquid；The surface of tissue sample is arranged in photoacoustic imaging window 3-10, and adjusts the height of photoacoustic imaging window 3-10
Make the focus for focusing light in sample surfaces, and makes the spatial light 3-3 alignment tissue sample after collimation；
2) laser sending pulse laser, which enters optical fiber collimator 3-2 by multimode fibre 3-1, becomes the space after collimating
Light 3-3 is 1064nm, repetition rate 100kHz, the pulse laser subsequently into condenser lens 3-4, the pulse laser wavelength of sending
It is focused by condenser lens 3-4, focuses light and pass through MEMS mirror 3-6, be radiated at tissue sample 3-7, MEMS mirror 3-6 is fixed on scanning
Pedestal 3-9, and MEMS mirror 3-6 drives the 3-5 connection of line 3-14 and function generator by MEMS mirror, and tissue sample 3-7 is placed
At photoacoustic imaging window 3-10 about 2-3cm, photoacoustic signal is being inspired, and photoacoustic signal is by the coupling liquid in coupling slot
It is received afterwards by interior snooping head 3-8, interior snooping head 3-8 is connect by interior snooping head signal wire 3-13 with signal transmission system, is inside peeped
Probe signal line 3-13 and MEMS mirror driving line 3-14 are fixed on laparoscope outer tube wall 3-11, and illumination fiber optic bundle 3-12 can be with
White light source is provided, is taken pictures for CCD, as shown in Figure 2；
3) the snooping received photoacoustic signal of head 3-8 is acquired by oscillograph and lock-in amplifier, shows after amplifier amplifies in
Wave device and lock-in amplifier store the signal message of acquisition into calculating, and it is 0-6V, frequency that function generator, which issues two-way amplitude,
Rate is respectively the triangular signal control MEMS mirror 3-6 of 1Hz and 10Hz, so that MEMS mirror 3-6 does the scanning of " Z " font, thus real
Existing laser forms X-Y two-dimensional surface scanning area on tissue sample；
4) after oscillograph and lock-in amplifier have acquired whole signals, computer utilizes Matlab program by the signal of each point
Time is once integrated, the function of time of tissue sample surface vibration displacement is obtained；Obtain tissue sample surface vibration position
Move from it is above freezing rise to its maximum value when the time required to because elasticity modulus is different, the time needed for displacement arrival maximum value
It is different.And phase loss angle can be obtained by lock-in amplifier and utilize the time by above-mentioned formula (3) (10)
The tissue sample for calculating each point quantifies elasticity modulus and viscosity.According to the elasticity and viscosity of calculating, biology is reconstructed
The two-dimension elastic and viscosity figure of tissue, as shown in Figure 4 and Figure 5.Fig. 4 and Fig. 5 is respectively the optoacoustic elasticity and viscosity of Agar samples
Figure, the biggish part of agar concentration it can be seen from Fig. 4 and Fig. 5, elasticity number is larger and viscosity value is smaller；Agar concentration is smaller
Part, elasticity number is smaller and viscosity value is larger.
The above, only the invention patent preferred embodiment, but the scope of protection of the patent of the present invention is not limited to
This, anyone skilled in the art is in the range disclosed in the invention patent, according to the present invention the skill of patent
Art scheme and its inventive concept are subject to equivalent substitution or change, belong to the scope of protection of the patent of the present invention.
Claims (9)
1. a kind of device for the optoacoustic laparoscope for detecting elasticity and viscosity simultaneously, it is characterised in that: including laser transmitting system, light
Coupled system, laparoscope system, signal transmission system and signal processing system；The laser transmitting system, optically coupled system,
Laparoscope system, signal transmission acquisition system, signal processing system are linked in sequence according to the transmission direction of optical signal；The laser
Emission system uses laser；The optically coupled system includes coupler, and coupler is connected by multimode fibre and laparoscope system
It connects；The laparoscope system includes collimator, condenser lens, function generator, MEMS mirror and interior snooping head, the collimator,
Condenser lens, MEMS mirror are linked in sequence, and the function generator is connect with MEMS mirror, and the interior snooping head is connected to signal transmission
System, the interior snooping head are ultrasonic detector；The signal transmission system includes amplifier, oscillograph, lock-in amplifier；Institute
Stating signal processing system is computer, the ultrasonic detector, amplifier, oscillograph, lock-in amplifier and computer successively phase
Even, the computer is equipped with acquisition control and signal processing system；
When test, tissue sample is placed on platform, and is immersed in the coupling liquid of coupling slot；The laparoscope system setting exists
Right above tissue sample, and laparoscope is without departing from tissue sample surface, the pulse laser that the laser issues by optical fiber and
Condenser lens focuses, and impinges upon on tissue sample；The ultrasonic detector is directed at tissue sample, and the lower end of ultrasonic detector enters
In the coupling liquid of coupling slot, the photoacoustic signal that tissue sample is inspired is received；MEMS mirror control in the laparoscope system
Pulse laser carries out point by point scanning tissue sample, specifically:
1) tissue sample is placed on sample cell, and be immersed in the coupling liquid of coupling slot；By laparoscope setting in tissue sample
Right above product, and the height for adjusting laparoscope makes the focus of laparoscope without departing from tissue sample surface；Ultrasonic detector is aligned
Tissue sample, and enter the lower end of ultrasonic detector in the coupling liquid of coupling slot；
2) laser issues pulse laser, which is focused by multimode fibre, collimator and condenser lens, is formed and is focused
Light beam impinges upon on MEMS mirror, and laser is impinged upon on tissue sample by MEMS mirror, inspires photoacoustic signal, photoacoustic signal is through overcoupling
It is received after coupling liquid in slot by interior snooping head；
3) snooping received photoacoustic signal is acquired after amplifier amplifies by oscillograph and lock-in amplifier in, oscillograph and
Lock-in amplifier stores the signal message of acquisition into computer, function generator control MEMS mirror make pulse laser carry out by
Spot scan sample, and X-Y two-dimensional surface scanning area is formed on corresponding tissue sample；
4) after oscillograph has acquired whole signals, computer is once integrated the signal of each point to the time, obtains tissue sample
The function of time of product surface vibration displacement；Obtain tissue sample surface vibration displacement from it is above freezing rise to its maximum value when taken
Between, elasticity modulus is quantified using the tissue sample that the time calculates each point；According to the quantitative elasticity modulus of calculating, tissue is reconstructed
The quantitative elastic two dimensional image of sample obtains optoacoustic viscoelastic image finally by the phase information that lock-in amplifier acquires, viscosity
Coefficient, which passes through, combines obtained elasticity modulus and the loss angle based on Kelvin-Voigt model to obtain.
2. a kind of device of optoacoustic laparoscope for detecting elasticity and viscosity simultaneously according to claim 1, it is characterised in that:
The pulse laser wavelength that the laser issues is 400~2500nm, and pulse width is 1~50ns, repetition rate be 1Hz~
100kHz。
3. a kind of device of optoacoustic laparoscope for detecting elasticity and viscosity simultaneously according to claim 1, it is characterised in that:
The core diameter of the multimode fibre is 62.5um.
4. a kind of device of optoacoustic laparoscope for detecting elasticity and viscosity simultaneously according to claim 1, it is characterised in that:
The MEMS mirror operating voltage 6V, diameter 4.5mm, maximum scan range are 3cm × 3cm.
5. a kind of device of optoacoustic laparoscope for detecting elasticity and viscosity simultaneously according to claim 1, it is characterised in that:
It is 0-9V that the function generator, which can produce two-way amplitude, and frequency range is the triangular signal of 0-1000Hz.
6. a kind of device of optoacoustic laparoscope for detecting elasticity and viscosity simultaneously according to claim 1, it is characterised in that:
The dominant frequency of the ultrasonic detector is 3MHz, and volume is 3mm × 3mm × 5mm；Bandwidth is 1.8-4.2MHz.
7. a kind of device of optoacoustic laparoscope for detecting elasticity and viscosity simultaneously according to claim 1, it is characterised in that:
The sample rate of the oscillograph is 2.5GHz, and the sample rate of lock-in amplifier is 102KHz, the acquisition control of the computer installation
System and signal processing system are formed using Labview and Matlab programming.
8. a kind of device of optoacoustic laparoscope for detecting elasticity and viscosity simultaneously according to claim 1, it is characterised in that:
In step (4), the signal of each point is carried out processing to the time and referred to by the computer:
Computer is once integrated the signal of each point to the time using Matlab program.
9. a kind of device of optoacoustic laparoscope for detecting elasticity and viscosity simultaneously according to claim 1, it is characterised in that:
In step (4), the tissue sample for calculating each point quantifies elasticity modulus, using following formula:
Wherein, ρ is biological tissue density, and R is laser facula radius, tmaxIt is risen to for tissue sample surface vibration displacement from above freezing
The time required to when its maximum value.
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