CN106676149A - Method for preparing xylooligosaccharide from bamboo sawdust - Google Patents
Method for preparing xylooligosaccharide from bamboo sawdust Download PDFInfo
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- CN106676149A CN106676149A CN201611223043.1A CN201611223043A CN106676149A CN 106676149 A CN106676149 A CN 106676149A CN 201611223043 A CN201611223043 A CN 201611223043A CN 106676149 A CN106676149 A CN 106676149A
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- oligosaccharide
- xylo
- bamboo scraps
- xylooligosaccharide
- xylan
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/12—Disaccharides
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Abstract
The invention discloses a method for preparing xylooligosaccharide from bamboo sawdust. Fresh bamboo sawdust is dried, crushed and sieved, then an alkaline liquid is added, ultrasonic extraction and filtration are performed, a filtrate is subjected to extraction with an ethyl alcohol method, cold storage and centrifugation are performed, crude xylan is obtained, xylanase and a buffer solution are added to the crude xylan, the mixture reacts under a certain condition for a period, and an xylooligosaccharide solution is obtained; xylooligosaccharide powder is obtained by performing spray drying on the xylooligosaccharide solution. The method is low in cost, simple and convenient, the obtained xylooligosaccharide has multiple physiological functions of promoting beneficial bacteria such as bifidobacterium and the like in animals to proliferate, inhibiting harmful bacteria, reducing decayed substances in intestines and the like, so that animal health is improved in multiple aspects, the xylooligosaccharide can be used as an animal feed additive, the use of chemical additives such as antibiotics and the like is reduced, and the xylooligosaccharide has significant social, society and environmental benefits.
Description
Technical field：
The invention mainly relates to a kind of method that utilization bamboo scraps prepare xylo-oligosaccharide, is mainly used in animal feed additive.
Background technology：
With growth in the living standard, people rise increasingly to the consumption figure of the animal foods such as meat, egg, milk, greatly stimulate
The development of aquaculture.Because there is disease-resistant and growth promotion to make to animal for antibiotic, hormone and heavy metal element copper, zinc, arsenic etc.
With, therefore, in order to improve the premunition and productivity of cultivated animals, the universal high dose of addition in violation of rules and regulations is anti-in feed addictive
Raw element and heavy metal, so as to cause animal food in have antibiotic and heavy-metal residual, health is worked the mischief.Together
When, the also antibiotic containing high concentration and heavy metal in the excreta of animal cause directly or indirectly pollution to environment.Therefore,
Few with or without antibiotic, heavy metal in feed, the natural feed addictive for developing safety is the certainty of modern feed industry development
Trend.
The bamboo resource very abundant of China, either bamboo grove class resource situation, bamboo grove cultivated area scale or bamboo grove store
Product output occupies global first place, there is the title of " bamboo kingdom ".The a large amount of bamboo scraps for producing during China's bamboo processing at present
Substantially all go out of use, averagely less than half, even less than a quarter, these give up some bamboo wood class product utilization rates its utilization rate
Abandon material major part be incinerated, process of banking up, not only economic benefit is low but also causes the pollution of environment and the waste of resource.Cause
This, the recycling of bamboo scraps is significant
Contain xylan in bamboo scraps, its content reaches 20% or so, and xylan can change into oligomeric wood by the hydrolysis of enzyme
Sugar.Xylo-oligosaccharide is a kind of functional oligomerization carbohydrate prebiotics, breeds, presses down with beneficial bacterium in the animal bodies such as Bifidobacterium is promoted
Various physiologic sexual functions such as harmful bacteria processed and reduction enteral corrupt substance, so as to improve host animal health from many aspects, can use
Make animal feed additive.
It is contemplated that preparing xylo-oligosaccharide for feed addictive using bamboo scraps, the chemistry such as antibiotic, heavy metal are reduced
The use of additive, safe to food and environmental protection has great importance.
The content of the invention：
Present invention aims to the drawbacks of bamboo scraps yield is big, resource utilization rate is low in China's bamboo process, grinds
Study carefully a kind of method that utilization bamboo scraps prepare xylo-oligosaccharide, be applied to feed addictive, to reduce the waste of resource, substitute or
The use of the poisonous and harmful chemical addition agent such as antibiotic, heavy metal in livestock and poultry cultivation is reduced, antibiotic in animal product is reduced
Residual, be conducive to food security and environmental protection, with realistic meaning.
The present invention is achieved by the following technical solutions：
A kind of method that utilization bamboo scraps prepare xylo-oligosaccharide, it is characterised in that carry out according to the following steps successively：
（1）, by fresh bamboo scraps crushed after being dried, cross 20~100 mesh sieves, obtain bamboo scraps powder；Weigh a certain amount of bamboo scraps powder
In plastic containers, according to solid-to-liquid ratio 1:10~15 addition concentration are 3~8% NaOH solution, ultrasonic at a temperature of 50~80 DEG C
After extracting 15~45min, filtered with 200~400 mesh nylon mesh, obtain filtrate；
（2）, in filtrate add 2~5 times of volumes 95% ethanol, at 4 DEG C refrigerate 10~15h, 3000~5000r/min from
10~15min of the heart, supernatant is poured out, and must be precipitated；The step of supernatant dissolves-refrigerate-be centrifuged according to above-mentioned ethanol repetition 1~
2 times, merge precipitation, it is so dry that slightly to carry xylan, it is standby；
（3）, weigh a certain amount of xylan that slightly carries and be placed in suitable container, appropriate xylan is then added in container
Enzyme, adds the disodium hydrogen phosphate-citric acid solution of a certain amount of pH=4~6, anti-at 30~50 DEG C in shaking bath
2.5~4.5h is answered, xylo-oligosaccharide enzymolysis solution is obtained, the addition of described zytase is 400~800IU/g xylans；
（4）, by xylo-oligosaccharide enzymolysis solution be spray-dried, obtain xylo-oligosaccharide powder.
The method that a kind of described utilization bamboo scraps prepare xylo-oligosaccharide, it is characterised in that：Step（1）Described bamboo scraps are crushed
40 mesh sieves are crossed afterwards.
The method that a kind of described utilization bamboo scraps prepare xylo-oligosaccharide, it is characterised in that：Step（1）Described bamboo scraps powder
With the solid-to-liquid ratio 1 of NaOH solution:12, the concentration of described NaOH solution is 5%.
The method that a kind of described utilization bamboo scraps prepare xylo-oligosaccharide, it is characterised in that：Step（1）Described ultrasonic extraction
Temperature is 60 DEG C, and the ultrasonic extraction time is 30min.
The method that a kind of described utilization bamboo scraps prepare xylo-oligosaccharide, it is characterised in that：Step（3）The cushioning liquid
Addition is：Xylan is slightly carried in per 1 liter of cushioning liquid containing 30~60g.
Xylo-oligosaccharide prepared by said method of the present invention, can be used for additive for farm animal feed.
It is an advantage of the invention that：
The present invention extracts the xylan in bamboo scraps first with dilute alkaline soln, then with enzymatic isolation method prepares xylo-oligosaccharide again.This
Bright method is with low cost, simple and easy, and the xylo-oligosaccharide of acquisition has beneficial bacterium propagation, suppression in the animal bodies such as promotion Bifidobacterium
Various physiologic sexual functions such as harmful bacteria processed and reduction enteral corrupt substance, so as to improve animal health from many aspects, can use start
Thing feed addictive, can reduce the use of the chemical addition agents such as antibiotic, with significant economic, society and environmental benefit.
Description of the drawings
Fig. 1 is xylo-oligosaccharide standard specimen（0-1）And enzymolysis liquid (1-5) mass spectrogram.
Specific embodiment：
A kind of method that utilization bamboo scraps prepare xylo-oligosaccharide, it is characterised in that：Carry out according to the following steps successively：
（1）, by fresh bamboo scraps crushed after being dried, cross 40 mesh sieves, obtain bamboo scraps powder；A certain amount of bamboo scraps powder is weighed in plastics
In container, according to solid-to-liquid ratio 1:12 add 5% NaOH solution, after ultrasonic extraction 30min at 60 DEG C, are sieved through with 300 mesh nylon
Filter, obtains filtrate；
（2）, in filtrate add 3 times of volumes 95% ethanol（Can the recycling after being concentrated by evaporation）, refrigerate at 4 DEG C
12h, 4500r/min are centrifuged 15min, and supernatant is poured out, and must precipitate, and supernatant dissolves according to above-mentioned ethanol -- and it is cold at 4 DEG C
Hide -- repeat 1~2 time the step of centrifugation, merge precipitation, after being dried slightly carry xylan, it is standby；
（3）, weigh and slightly carry xylan and zytase in right amount in suitable container, add the disodium hydrogen phosphate and lemon of pH=5.5
Lemon acid buffering solution so that slightly carry the mass/volume ratio of xylan and cushioning liquid for 45g/L, the wherein addition of zytase
Measure as 600IU/g substrates, 3h is reacted at 40 DEG C in shaking bath, obtain xylo-oligosaccharide enzymolysis solution；
（4）, by xylo-oligosaccharide enzymolysis solution be spray-dried, obtain xylo-oligosaccharide powder.
Xylo-oligosaccharide enzymolysis product is analyzed using UPLC-MS/MS methods, is as a result seen Fig. 1, as seen from Figure 1,
The xylo-oligosaccharide that bamboo scraps enzymolysis is obtained be mainly xylobiose, xylotriose and Xylotetrose (retention time of xylobiose in 3.31 min,
The retention time of xylotriose is 3.70 min, and the retention time of Xylotetrose is 3.98 min), wherein xylobiose and xylotriose are accounted for
67.23%, xylo-oligosaccharide accounts for the 82% of enzymolysis product.
The overall conversion for obtaining after testing preparing xylo-oligosaccharide by bamboo scraps enzymolysis is 3.69%, i.e. 100kg bamboo scraps powder energy
Prepared 3.69kg xylo-oligosaccharides.
Claims (6)
1. a kind of method that utilization bamboo scraps prepare xylo-oligosaccharide, it is characterised in that carry out according to the following steps successively：
（1）, by fresh bamboo scraps crushed after being dried, cross 20~100 mesh sieves, obtain bamboo scraps powder；Weigh a certain amount of bamboo scraps powder
In plastic containers, according to solid-to-liquid ratio 1:10~15 addition concentration are 3~8% NaOH solution, ultrasonic at a temperature of 50~80 DEG C
After extracting 15~45min, filtered with 200~400 mesh nylon mesh, obtain filtrate；
（2）, in filtrate add 2~5 times of volumes 95% ethanol, at 4 DEG C refrigerate 10~15h, 3000~5000r/min from
10~15min of the heart, supernatant is poured out, and must be precipitated；The step of supernatant dissolves-refrigerate-be centrifuged according to above-mentioned ethanol repetition 1~
2 times, merge precipitation, it is so dry that slightly to carry xylan, it is standby；
（3）, weigh a certain amount of xylan that slightly carries and be placed in suitable container, appropriate xylan is then added in container
Enzyme, adds the disodium hydrogen phosphate-citric acid solution of a certain amount of pH=4~6, anti-at 30~50 DEG C in shaking bath
2.5~4.5h is answered, xylo-oligosaccharide enzymolysis solution is obtained, the addition of described zytase is 400~800IU/g xylans；
（4）, by xylo-oligosaccharide enzymolysis solution be spray-dried, obtain xylo-oligosaccharide powder.
2. the method that a kind of utilization bamboo scraps according to claim 1 prepare xylo-oligosaccharide, it is characterised in that：Step（1）Institute
The bamboo scraps stated cross 40 mesh sieves after crushing.
3. the method that a kind of utilization bamboo scraps according to claim 1 prepare xylo-oligosaccharide, it is characterised in that：Step（1）Institute
The bamboo scraps powder stated and the solid-to-liquid ratio 1 of NaOH solution:12, the concentration of described NaOH solution is 5%.
4. the method that a kind of utilization bamboo scraps according to claim 1 prepare xylo-oligosaccharide, it is characterised in that：Step（1）Institute
The ultrasonic extraction temperature stated is 60 DEG C, and the ultrasonic extraction time is 30min.
5. the method that a kind of utilization bamboo scraps according to claim 1 prepare xylo-oligosaccharide, it is characterised in that：Step（3）Institute
The addition for stating cushioning liquid is：Xylan is slightly carried in per 1 liter of cushioning liquid containing 30~60g.
6. xylo-oligosaccharide obtained in the method that xylo-oligosaccharide is prepared by the use of bamboo scraps described in claim 1 is added as animal and fowl fodder
The application of agent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611223043.1A CN106676149A (en) | 2016-12-27 | 2016-12-27 | Method for preparing xylooligosaccharide from bamboo sawdust |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611223043.1A CN106676149A (en) | 2016-12-27 | 2016-12-27 | Method for preparing xylooligosaccharide from bamboo sawdust |
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| Publication Number | Publication Date |
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| CN106676149A true CN106676149A (en) | 2017-05-17 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201611223043.1A Pending CN106676149A (en) | 2016-12-27 | 2016-12-27 | Method for preparing xylooligosaccharide from bamboo sawdust |
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101736057A (en) * | 2008-11-06 | 2010-06-16 | 上海市农业科学院 | Method for preparing lower polyxylose with bamboo wood scrap material |
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- 2016-12-27 CN CN201611223043.1A patent/CN106676149A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101736057A (en) * | 2008-11-06 | 2010-06-16 | 上海市农业科学院 | Method for preparing lower polyxylose with bamboo wood scrap material |
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Application publication date: 20170517 |